pycom06g01200

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003697
Physical Location & Seq
Reverse (-)
1236492 .. 1240784
4293 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g01200.2

Sequence Viewer

Length: 987 bp
ATGAGCGGGTCGTCGGTGGGTACTGGAGGTCACGCGCCGCTCTTCACAGTGGCGCAATGGGCTGAGATGGAGCATCAAGCTTTGATATTCAAGTATCTCAAGGCAGGAGTCCCTGTTCCTTCTGAGCTCCTCGCCCCTATTCGCACCAGTTTCAACCTCATTTCCCCCAATTTCTTACCTCACCACCCCACCTGGGGTTATGCTTCCTATTGCGGGAAGAAGATAGACCCAGAGCCGGGACGGTGCCGGAGGACGGATGGCAAGAAATGGAGGTGCTCCAAAGATGCATATCAGGACTCCAAGTATTGCGAGCGGCACATGAACCGCGGCCGCAATCGTTCAAGAAAGCTTGTGGAATCACAAACTGCCTCTCAATCTTTGTCAACTGTGACGTCAGACAGTGTGATCGGGAGCTCCAGCCACGGTGGGAGCTTCCAGACCATGCCCTTACACTCAATTGGAAATAGTGGAGGCCTATGCTTTGGAGGCAATGGATCTGAGTTGAAGATGGAGGCTATACCCTATGGGGTGTCTAACAGGGGAACCAGGGGGTGCAATAGATCTGGATCAGAAGAACACAATTTCATGTCAGAAGCTAAGAGGTGTGTTCAGGCGCACAGTACTGTAGACAGTCCGTGGCGTCTAATGGAGCCTCAAGTTCCCTTAAAATCAGAATTGAGAAATAGTTCTCTTTGGCTAAACAACAGCTCTCAGCTACAGACATTACAAACTATGGAGTCATTGAGCGGTGCTGCTGCAATCACCAAACAGGAGTATGTTGGTGGAGAATTTGGCTTACCAGGATCTCAAAAACATGAACAAAATTCTCTTCAGCCTTTATTTGATGAGTGGCCTAAGTCGAGGGATCTGGGGTTCCATCTGAATGATCATAGATCAAACACGACTGAGCTGTCAATGTCTAATGCAGGGGCCACCCCTAAATATTGCACAAGGAGTGCTGAGTCACCCAGTGATGCCTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QLQ WRC

Protein Analysis

329

Amino Acids

36.01

Weight (kDa)

8.72

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 910
AatII GACGTC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 243
AccBSI CCGCTC 4 cut(s) 6, 40, 313, 747
AccI GTMKAC 1 cut(s) 627
AccII CGCG 2 cut(s) 35, 327
AciI CCGC 8 cut(s) 6, 38, 213, 313, 325, 327, 331, 747
AclWI GGATC 4 cut(s) 502, 574, 811, 873
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 788, 823
AcuI CTGAAG 1 cut(s) 815
AcyI GRCGYC 2 cut(s) 392, 640
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 2 cut(s) 22, 622
AfiI CCNNNNNNNGG 6 cut(s) 193, 194, 213, 235, 236, 253
AgsI TTSAA 4 cut(s) 91, 154, 342, 505
AjnI CCWGG 3 cut(s) 191, 545, 799
AloI GAACNNNNNNTCC 4 cut(s) 99, 131, 857, 889
AluBI AGCT 9 cut(s) 80, 127, 349, 414, 432, 596, 708, 715, 910
AluI AGCT 9 cut(s) 80, 127, 349, 414, 432, 596, 708, 715, 910
Alw21I GWGCWC 3 cut(s) 129, 278, 416
AlwI GGATC 4 cut(s) 502, 574, 811, 873
AoxI GGCC 4 cut(s) 328, 472, 851, 930
ApeKI GCWGC 2 cut(s) 752, 755
ApoI RAATTY 2 cut(s) 788, 823
Asp700I GAANNNNTTC 1 cut(s) 685
AspLEI GCGC 3 cut(s) 37, 55, 616
AspS9I GGNCC 1 cut(s) 930
AsuC2I CCSGG 1 cut(s) 237
AsuHPI GGTGA 3 cut(s) 173, 754, 957
BanI GGYRCC 1 cut(s) 243
BanII GRGCYC 2 cut(s) 129, 416
Bbv12I GWGCWC 3 cut(s) 129, 278, 416
BbvI GCAGC 2 cut(s) 739, 742
BccI CCATC 4 cut(s) 61, 251, 502, 885
BciT130I CCWGG 3 cut(s) 193, 547, 801
BclI TGATCA 1 cut(s) 886
BcnI CCSGG 1 cut(s) 237
BfmI CTRYAG 2 cut(s) 624, 716
BglII AGATCT 1 cut(s) 560
BisI GCNGC 6 cut(s) 38, 314, 328, 331, 753, 756
BlsI GCNGC 6 cut(s) 39, 315, 329, 332, 754, 757
BmcAI AGTACT 1 cut(s) 622
Bme1390I CCNGG 4 cut(s) 193, 237, 547, 801
BmgT120I GGNCC 1 cut(s) 930
BmiI GGNNCC 5 cut(s) 245, 544, 651, 875, 931
BmrFI CCNGG 4 cut(s) 193, 237, 547, 801
BmrI ACTGGG 1 cut(s) 963
BmsI GCATC 3 cut(s) 82, 274, 964
BmuI ACTGGG 1 cut(s) 963
BpmI CTGGAG 2 cut(s) 45, 400
BpuEI CTTGAG 2 cut(s) 83, 639
BpuMI CCSGG 1 cut(s) 237
BsaBI GATNNNNATC 2 cut(s) 288, 565
BsaHI GRCGYC 2 cut(s) 392, 640
BsaJI CCNNGG 5 cut(s) 192, 325, 421, 546, 635
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 6 cut(s) 193, 194, 213, 235, 236, 253
Bse1I ACTGG 3 cut(s) 28, 147, 969
Bse3DI GCAATG 2 cut(s) 62, 496
Bse8I GATNNNNATC 2 cut(s) 288, 565
BseBI CCWGG 3 cut(s) 193, 547, 801
BseDI CCNNGG 5 cut(s) 192, 325, 421, 546, 635
BseGI GGATG 1 cut(s) 262
BseJI GATNNNNATC 2 cut(s) 288, 565
BseLI CCNNNNNNNGG 6 cut(s) 193, 194, 213, 235, 236, 253
BseMI GCAATG 2 cut(s) 62, 496
BseMII CTCAG 6 cut(s) 54, 114, 489, 725, 897, 951
BseNI ACTGG 3 cut(s) 28, 147, 969
BseRI GAGGAG 1 cut(s) 119
BseX3I CGGCCG 1 cut(s) 328
BseXI GCAGC 2 cut(s) 739, 742
Bsh1236I CGCG 2 cut(s) 35, 327
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 4 cut(s) 330, 474, 853, 932
BshNI GGYRCC 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 3 cut(s) 129, 278, 416
BsiSI CCGG 2 cut(s) 236, 247
BslFI GGGAC 2 cut(s) 95, 252
BslI CCNNNNNNNGG 6 cut(s) 193, 194, 213, 235, 236, 253
BsmFI GGGAC 2 cut(s) 95, 252
BsnI GGCC 4 cut(s) 330, 474, 853, 932
Bsp1286I GDGCHC 3 cut(s) 129, 278, 416
Bsp143I GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
BspACI CCGC 8 cut(s) 6, 38, 213, 313, 325, 327, 331, 747
BspANI GGCC 4 cut(s) 330, 474, 853, 932
BspCNI CTCAG 6 cut(s) 55, 115, 490, 724, 898, 952
BspFNI CGCG 2 cut(s) 35, 327
BspLI GGNNCC 5 cut(s) 245, 544, 651, 875, 931
BspPI GGATC 4 cut(s) 502, 574, 811, 873
BspQI GCTCTTC 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 243
BsrBI CCGCTC 4 cut(s) 6, 40, 313, 747
BsrDI GCAATG 2 cut(s) 62, 496
BsrI ACTGG 3 cut(s) 28, 147, 969
BssECI CCNNGG 5 cut(s) 192, 325, 421, 546, 635
BssMI GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
BssNI GRCGYC 2 cut(s) 392, 640
Bst2UI CCWGG 3 cut(s) 193, 547, 801
Bst4CI ACNGT 8 cut(s) 49, 243, 388, 401, 425, 620, 625, 632
Bst6I CTCTTC 2 cut(s) 47, 834
BstACI GRCGYC 2 cut(s) 392, 640
BstC8I GCNNGC 1 cut(s) 311
BstDEI CTNAG 8 cut(s) 63, 123, 498, 597, 711, 855, 906, 960
BstDSI CCRYGG 3 cut(s) 325, 421, 635
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 2 cut(s) 35, 327
BstHHI GCGC 3 cut(s) 37, 55, 616
BstKTI GATC 8 cut(s) 408, 497, 563, 569, 806, 868, 889, 896
BstMBI GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 59, 486
BstNI CCWGG 3 cut(s) 193, 547, 801
BstSCI CCNGG 4 cut(s) 191, 235, 545, 799
BstSFI CTRYAG 2 cut(s) 624, 716
BstUI CGCG 2 cut(s) 35, 327
BstV1I GCAGC 2 cut(s) 739, 742
BstX2I RGATCY 4 cut(s) 494, 560, 803, 865
BstYI RGATCY 4 cut(s) 494, 560, 803, 865
BstZI CGGCCG 1 cut(s) 328
BsuRI GGCC 4 cut(s) 330, 474, 853, 932
BtgI CCRYGG 3 cut(s) 325, 421, 635
BtsCI GGATG 1 cut(s) 262
BtsIMutI CAGTG 3 cut(s) 54, 406, 976
Cac8I GCNNGC 1 cut(s) 311
CciNI GCGGCCGC 1 cut(s) 328
CfoI GCGC 3 cut(s) 37, 55, 616
Cfr13I GGNCC 1 cut(s) 930
Cfr42I CCGCGG 1 cut(s) 328
CseI GACGC 1 cut(s) 629
Csp6I GTAC 2 cut(s) 21, 621
CviAII CATG 4 cut(s) 319, 442, 586, 815
CviQI GTAC 2 cut(s) 21, 621
DdeI CTNAG 8 cut(s) 63, 123, 498, 597, 711, 855, 906, 960
DpnI GATC 8 cut(s) 407, 496, 562, 568, 805, 867, 888, 895
DpnII GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
DraIII CACNNNGTG 1 cut(s) 971
DrdI GACNNNNNNGTC 1 cut(s) 910
DseDI GACNNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 1 cut(s) 328
EagI CGGCCG 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 47, 834
EarI CTCTTC 2 cut(s) 47, 834
Ecl136II GAGCTC 2 cut(s) 127, 414
EclXI CGGCCG 1 cut(s) 328
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 2 cut(s) 129, 416
Eco52I CGGCCG 1 cut(s) 328
Eco53kI GAGCTC 2 cut(s) 127, 414
Eco57I CTGAAG 1 cut(s) 815
EcoICRI GAGCTC 2 cut(s) 127, 414
EcoRII CCWGG 3 cut(s) 191, 545, 799
EcoT22I ATGCAT 1 cut(s) 289
EcoT38I GRGCYC 2 cut(s) 129, 416
FaeI CATG 4 cut(s) 322, 445, 589, 818
FaqI GGGAC 2 cut(s) 95, 252
FatI CATG 4 cut(s) 318, 441, 585, 814
FauI CCCGC 1 cut(s) 206
FbaI TGATCA 1 cut(s) 886
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 6 cut(s) 38, 314, 328, 331, 753, 756
FokI GGATG 1 cut(s) 269
FriOI GRGCYC 2 cut(s) 129, 416
Fsp4HI GCNGC 6 cut(s) 38, 314, 328, 331, 753, 756
GlaI GCGC 3 cut(s) 36, 54, 615
GluI GCNGC 6 cut(s) 38, 314, 328, 331, 753, 756
GsuI CTGGAG 2 cut(s) 45, 400
HaeIII GGCC 4 cut(s) 330, 474, 853, 932
HapII CCGG 2 cut(s) 236, 247
HgaI GACGC 1 cut(s) 629
HhaI GCGC 3 cut(s) 37, 55, 616
Hin1I GRCGYC 2 cut(s) 392, 640
Hin1II CATG 4 cut(s) 322, 445, 589, 818
Hin6I GCGC 3 cut(s) 35, 53, 614
HinP1I GCGC 3 cut(s) 35, 53, 614
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HindIII AAGCTT 2 cut(s) 78, 347
HinfI GANTC 5 cut(s) 108, 296, 356, 737, 962
HpaII CCGG 2 cut(s) 236, 247
HphI GGTGA 3 cut(s) 173, 754, 957
Hpy166II GTNNAC 2 cut(s) 384, 628
Hpy188I TCNGA 7 cut(s) 124, 397, 499, 571, 592, 673, 882
Hpy188III TCNNGA 5 cut(s) 293, 342, 409, 436, 564
Hpy8I GTNNAC 2 cut(s) 384, 628
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 129
HpyCH4III ACNGT 8 cut(s) 49, 243, 388, 401, 425, 620, 625, 632
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 5 cut(s) 287, 555, 758, 926, 948
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 486
HpyF3I CTNAG 8 cut(s) 63, 123, 498, 597, 711, 855, 906, 960
HpySE526I ACGT 1 cut(s) 392
Hsp92I GRCGYC 2 cut(s) 392, 640
Hsp92II CATG 4 cut(s) 322, 445, 589, 818
HspAI GCGC 3 cut(s) 35, 53, 614
Ksp22I TGATCA 1 cut(s) 886
KspI CCGCGG 1 cut(s) 328
Kzo9I GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 7 cut(s) 70, 132, 281, 411, 419, 429, 649
Lsp1109I GCAGC 2 cut(s) 739, 742
LweI GCATC 3 cut(s) 82, 274, 964
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 3 cut(s) 29, 388, 963
MalI GATC 8 cut(s) 407, 496, 562, 568, 805, 867, 888, 895
MbiI CCGCTC 4 cut(s) 6, 40, 313, 747
MboI GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
MboII GAAGA 6 cut(s) 34, 229, 232, 517, 584, 821
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 4 cut(s) 494, 560, 803, 865
MhlI GDGCHC 3 cut(s) 129, 278, 416
MluCI AATT 6 cut(s) 169, 456, 580, 674, 788, 823
MlyI GAGTC 4 cut(s) 117, 290, 746, 971
Mph1103I ATGCAT 1 cut(s) 289
MroXI GAANNNNTTC 1 cut(s) 685
MseI TTAA 1 cut(s) 665
MslI CAYNNNNRTG 1 cut(s) 882
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 2 cut(s) 236, 247
MspR9I CCNGG 4 cut(s) 193, 237, 547, 801
MunI CAATTG 1 cut(s) 456
MvaI CCWGG 3 cut(s) 193, 547, 801
MvnI CGCG 2 cut(s) 35, 327
MwoI GCNNNNNNNGC 2 cut(s) 59, 486
NciI CCSGG 1 cut(s) 237
NdeII GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
NlaIII CATG 4 cut(s) 322, 445, 589, 818
NlaIV GGNNCC 5 cut(s) 245, 544, 651, 875, 931
NmuCI GTSAC 3 cut(s) 29, 388, 963
NotI GCGGCCGC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 289
PceI AGGCCT 1 cut(s) 474
PciSI GCTCTTC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 685
PfeI GAWTC 1 cut(s) 356
PkrI GCNGC 6 cut(s) 39, 315, 329, 332, 754, 757
PleI GAGTC 4 cut(s) 116, 290, 745, 970
PpsI GAGTC 4 cut(s) 116, 290, 745, 970
Psp124BI GAGCTC 2 cut(s) 129, 416
Psp6I CCWGG 3 cut(s) 191, 545, 799
PspGI CCWGG 3 cut(s) 191, 545, 799
PspN4I GGNNCC 5 cut(s) 245, 544, 651, 875, 931
PspPI GGNCC 1 cut(s) 930
PsuI RGATCY 4 cut(s) 494, 560, 803, 865
RsaI GTAC 2 cut(s) 22, 622
RsaNI GTAC 2 cut(s) 21, 621
RseI CAYNNNNRTG 1 cut(s) 882
SacI GAGCTC 2 cut(s) 129, 416
SacII CCGCGG 1 cut(s) 328
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 665
SatI GCNGC 6 cut(s) 38, 314, 328, 331, 753, 756
Sau3AI GATC 8 cut(s) 405, 494, 560, 566, 803, 865, 886, 893
Sau96I GGNCC 1 cut(s) 930
ScaI AGTACT 1 cut(s) 622
SchI GAGTC 4 cut(s) 117, 290, 746, 971
ScrFI CCNGG 4 cut(s) 193, 237, 547, 801
SduI GDGCHC 3 cut(s) 129, 278, 416
SfaNI GCATC 3 cut(s) 82, 274, 964
SfcI CTRYAG 2 cut(s) 624, 716
Sfr303I CCGCGG 1 cut(s) 328
SgrBI CCGCGG 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 882
SmlI CTYRAG 2 cut(s) 98, 654
SmoI CTYRAG 2 cut(s) 98, 654
Sse9I AATT 6 cut(s) 169, 456, 580, 674, 788, 823
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 8 cut(s) 6, 38, 213, 313, 325, 327, 331, 747
SspI AATATT 1 cut(s) 944
SstI GAGCTC 2 cut(s) 129, 416
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 4 cut(s) 191, 235, 545, 799
TaaI ACNGT 8 cut(s) 49, 243, 388, 401, 425, 620, 625, 632
TaiI ACGT 1 cut(s) 395
TaqI TCGA 1 cut(s) 860
TasI AATT 6 cut(s) 169, 456, 580, 674, 788, 823
TatI WGTACW 1 cut(s) 620
TauI GCSGC 4 cut(s) 40, 316, 330, 333
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TscAI CASTG 3 cut(s) 54, 406, 976
TseFI GTSAC 3 cut(s) 29, 388, 963
TseI GCWGC 2 cut(s) 752, 755
Tsp45I GTSAC 3 cut(s) 29, 388, 963
TspDTI ATGAA 3 cut(s) 335, 574, 831
TspGWI ACGGA 2 cut(s) 269, 624
TspRI CASTG 3 cut(s) 54, 406, 976
XapI RAATTY 2 cut(s) 788, 823
XmiI GTMKAC 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 685
ZraI GACGTC 1 cut(s) 393
ZrmI AGTACT 1 cut(s) 622
Zsp2I ATGCAT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.