Rroxscaffold_4G00276550

QLQ

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003697
Physical Location & Seq
Forward (+)
121843 .. 124753
2911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276550.1

Sequence Viewer

Length: 1014 bp
ATGAATAGCTGCAGTACTGGAGGTGGGGGCGGGGTTGGGGTTGGGGGTAATAGTACTGGGAATCTGATGGGAATGGGGATGGGGATGAGGTCACCTTTTACAGTGTCTCAGTGGCAAGAGCTGGAGCATCAGGCCCTGATCTTCAAGTATATGATGGCAGGCATGCCTGTGCCTCCTGATCTCGTGCTCCCTATTCAGAAGAGTTTTGAGTCCATCTCTCAGAGGTTCTTCCATCATCCCACCATGGGTTATTGTTCCTTCTATGGGAAGAAGGTGGACCCGGAGCCAGGACGCTGCAGGAGGACTGATGGCAAGAAATGGAGGTGCTCAAAGGATGCATACCCCGACTCGAAGTACTGTGAGCGCCACATGCACCGTGGCCGCAACCGTTCAAGAAAGCCTGTGGAATCACAAACAATGACACAGTCATCGTCCACTGTGACATCATTGACTGTCACTGGAACCAGCAGTGGAACTGGGAGCTTCCAGACCCTTCCATTAAATTCCTTCGGGAATACCCAAGGCGCCAGTTCCGGAACCAATCAGTCTCATTATCAAATAGACTCTATTCCCTTTGGAATCCCGAACAAAGATTATAGGTATCTTCAAGGACTTAAACCTGAAATCGGTGAGCATAGTTTTTTCTCTAATACTTCAGGAAGCAACAGGGGTCTCCACATGGACTCACCAATAGACAACACATGGCCTCTGATGCCATCGAGAATTGCCTCATTTCCAGCATCAAAATCGGGTGATGGCTCCGTTTTGCAGAACGATTATCCACAGCATTCAATTTTCAATAATGACTTCCCCTCGGGGGAACCTTTGAAACAGGAAGGTCAGTCTCTCCGACCTTTCTTTGATGAATGGCCTAAAGGCCGAGATTCCTGGTCTGGTCTTGAGGATGAGAGATCCAACCAAAGCTCATTCTCCACAACCCAGCTCTCAATATCAATTCCAATGACTTCTTCCGACTTCTCTGCAACAAGTTCTCAATCTCCACATGATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QLQ WRC

Protein Analysis

337

Amino Acids

37.08

Weight (kDa)

7.71

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 32 - 66 4.5e-16 QLQ
WRC PF08879 94 - 136 3.9e-21 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 524
AccIII TCCGGA 1 cut(s) 533
AciI CCGC 2 cut(s) 30, 382
AclWI GGATC 1 cut(s) 906
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 1 cut(s) 502
AcuI CTGAAG 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 3 cut(s) 16, 55, 356
AfiI CCNNNNNNNGG 5 cut(s) 245, 264, 287, 626, 817
AgsI TTSAA 6 cut(s) 145, 393, 608, 792, 799, 829
AjnI CCWGG 2 cut(s) 286, 887
AjuI GAANNNNNNNTTGG 2 cut(s) 952, 984
AluBI AGCT 5 cut(s) 9, 121, 483, 924, 943
AluI AGCT 5 cut(s) 9, 121, 483, 924, 943
Alw21I GWGCWC 2 cut(s) 189, 329
Alw26I GTCTC 4 cut(s) 111, 552, 677, 849
AlwI GGATC 1 cut(s) 906
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 814
Aor13HI TCCGGA 1 cut(s) 533
AoxI GGCC 5 cut(s) 132, 379, 704, 869, 877
ApeKI GCWGC 2 cut(s) 9, 294
ApoI RAATTY 1 cut(s) 502
AspLEI GCGC 2 cut(s) 366, 527
AspS9I GGNCC 2 cut(s) 133, 277
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 4 cut(s) 84, 641, 678, 764
AvaI CYCGRG 1 cut(s) 814
AvaII GGWCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 524
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 2 cut(s) 189, 329
BbvI GCAGC 1 cut(s) 281
BccI CCATC 8 cut(s) 61, 73, 148, 221, 240, 302, 724, 749
BcgI CGANNNNNNTGC 4 cut(s) 729, 763, 962, 996
BciT130I CCWGG 2 cut(s) 288, 889
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 4 cut(s) 111, 552, 677, 849
BfmI CTRYAG 2 cut(s) 10, 295
BfoI RGCGCY 2 cut(s) 367, 528
BisI GCNGC 3 cut(s) 10, 295, 382
BlsI GCNGC 3 cut(s) 11, 296, 383
BmcAI AGTACT 3 cut(s) 16, 55, 356
Bme1390I CCNGG 3 cut(s) 281, 288, 889
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 814
BmgT120I GGNCC 2 cut(s) 133, 277
BmiI GGNNCC 7 cut(s) 279, 285, 463, 526, 538, 760, 822
BmrFI CCNGG 3 cut(s) 281, 288, 889
BmrI ACTGGG 2 cut(s) 66, 486
BmsI GCATC 4 cut(s) 136, 325, 702, 749
BmuI ACTGGG 2 cut(s) 66, 486
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 2 cut(s) 39, 143
BpuEI CTTGAG 1 cut(s) 920
BpuMI CCSGG 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 525
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 4 cut(s) 243, 376, 520, 813
BsaWI WCCGGW 1 cut(s) 533
Bsc4I CCNNNNNNNGG 5 cut(s) 245, 264, 287, 626, 817
Bse1I ACTGG 5 cut(s) 22, 61, 463, 481, 528
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 2 cut(s) 288, 889
BseDI CCNNGG 4 cut(s) 243, 376, 520, 813
BseGI GGATG 5 cut(s) 84, 90, 235, 340, 910
BseLI CCNNNNNNNGG 5 cut(s) 245, 264, 287, 626, 817
BseMII CTCAG 2 cut(s) 122, 233
BseNI ACTGG 5 cut(s) 22, 61, 463, 481, 528
BseXI GCAGC 1 cut(s) 281
BseYI CCCAGC 1 cut(s) 939
BshFI GGCC 5 cut(s) 134, 381, 706, 871, 879
BshNI GGYRCC 1 cut(s) 524
BsiHKAI GWGCWC 2 cut(s) 189, 329
BsiHKCI CYCGRG 1 cut(s) 814
BsiSI CCGG 2 cut(s) 281, 534
BslI CCNNNNNNNGG 5 cut(s) 245, 264, 287, 626, 817
BsmAI GTCTC 4 cut(s) 111, 552, 677, 849
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 5 cut(s) 134, 381, 706, 871, 879
Bso31I GGTCTC 1 cut(s) 677
BsoBI CYCGRG 1 cut(s) 814
Bsp1286I GDGCHC 2 cut(s) 189, 329
Bsp13I TCCGGA 1 cut(s) 533
Bsp143I GATC 3 cut(s) 138, 178, 911
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 2 cut(s) 30, 382
BspANI GGCC 5 cut(s) 134, 381, 706, 871, 879
BspCNI CTCAG 2 cut(s) 121, 232
BspEI TCCGGA 1 cut(s) 533
BspLI GGNNCC 7 cut(s) 279, 285, 463, 526, 538, 760, 822
BspMAI CTGCAG 2 cut(s) 14, 299
BspPI GGATC 1 cut(s) 906
BspT107I GGYRCC 1 cut(s) 524
BspTNI GGTCTC 1 cut(s) 677
BsrI ACTGG 5 cut(s) 22, 61, 463, 481, 528
BssECI CCNNGG 4 cut(s) 243, 376, 520, 813
BssMI GATC 3 cut(s) 138, 178, 911
BssNI GRCGYC 1 cut(s) 525
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 243, 520
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 2 cut(s) 288, 889
Bst4CI ACNGT 7 cut(s) 103, 359, 377, 389, 426, 439, 454
Bst6I CTCTTC 1 cut(s) 194
BstACI GRCGYC 1 cut(s) 525
BstC8I GCNNGC 2 cut(s) 160, 164
BstDEI CTNAG 2 cut(s) 108, 219
BstDSI CCRYGG 2 cut(s) 243, 376
BstEII GGTNACC 1 cut(s) 90
BstF5I GGATG 5 cut(s) 84, 90, 235, 340, 910
BstH2I RGCGCY 2 cut(s) 367, 528
BstHHI GCGC 2 cut(s) 366, 527
BstKTI GATC 3 cut(s) 141, 181, 914
BstMAI GTCTC 4 cut(s) 111, 552, 677, 849
BstMBI GATC 3 cut(s) 138, 178, 911
BstMWI GCNNNNNNNGC 2 cut(s) 370, 712
BstNI CCWGG 2 cut(s) 288, 889
BstNSI RCATGY 2 cut(s) 166, 373
BstPI GGTNACC 1 cut(s) 90
BstSCI CCNGG 3 cut(s) 279, 286, 887
BstSFI CTRYAG 2 cut(s) 10, 295
BstV1I GCAGC 1 cut(s) 281
BstX2I RGATCY 1 cut(s) 911
BstYI RGATCY 1 cut(s) 911
BsuRI GGCC 5 cut(s) 134, 381, 706, 871, 879
BtgI CCRYGG 2 cut(s) 243, 376
BtsCI GGATG 5 cut(s) 84, 90, 235, 340, 910
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 5 cut(s) 108, 116, 435, 456, 475
Cac8I GCNNGC 2 cut(s) 160, 164
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 2 cut(s) 366, 527
Cfr13I GGNCC 2 cut(s) 133, 277
CseI GACGC 1 cut(s) 300
Csp6I GTAC 3 cut(s) 15, 54, 355
CviAII CATG 6 cut(s) 163, 244, 370, 679, 702, 1004
CviQI GTAC 3 cut(s) 15, 54, 355
DdeI CTNAG 2 cut(s) 108, 219
DinI GGCGCC 1 cut(s) 526
DpnI GATC 3 cut(s) 140, 180, 913
DpnII GATC 3 cut(s) 138, 178, 911
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 243, 520
Eco31I GGTCTC 1 cut(s) 677
Eco47I GGWCC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 1 cut(s) 814
Eco91I GGTNACC 1 cut(s) 90
EcoO109I RGGNCCY 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 286, 887
EcoT14I CCWWGG 2 cut(s) 243, 520
EcoT22I ATGCAT 1 cut(s) 340
EgeI GGCGCC 1 cut(s) 526
EheI GGCGCC 1 cut(s) 526
ErhI CCWWGG 2 cut(s) 243, 520
FaeI CATG 6 cut(s) 166, 247, 373, 682, 705, 1007
FatI CATG 6 cut(s) 162, 243, 369, 678, 701, 1003
FauI CCCGC 1 cut(s) 23
Fnu4HI GCNGC 3 cut(s) 10, 295, 382
FokI GGATG 5 cut(s) 91, 97, 222, 347, 917
Fsp4HI GCNGC 3 cut(s) 10, 295, 382
GlaI GCGC 2 cut(s) 365, 526
GluI GCNGC 3 cut(s) 10, 295, 382
GsaI CCCAGC 1 cut(s) 943
GsuI CTGGAG 2 cut(s) 39, 143
HaeII RGCGCY 2 cut(s) 367, 528
HaeIII GGCC 5 cut(s) 134, 381, 706, 871, 879
HapII CCGG 2 cut(s) 281, 534
HgaI GACGC 1 cut(s) 300
HhaI GCGC 2 cut(s) 366, 527
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 6 cut(s) 166, 247, 373, 682, 705, 1007
Hin6I GCGC 2 cut(s) 364, 525
HinP1I GCGC 2 cut(s) 364, 525
HinfI GANTC 8 cut(s) 61, 209, 347, 407, 563, 579, 683, 884
HpaII CCGG 2 cut(s) 281, 534
HphI GGTGA 4 cut(s) 84, 641, 678, 764
Hpy166II GTNNAC 2 cut(s) 277, 435
Hpy188I TCNGA 6 cut(s) 66, 198, 222, 711, 851, 973
Hpy188III TCNNGA 9 cut(s) 176, 393, 487, 511, 534, 583, 657, 720, 899
Hpy8I GTNNAC 2 cut(s) 277, 435
HpyAV CCTTC 5 cut(s) 265, 268, 503, 517, 830
HpyCH4III ACNGT 7 cut(s) 103, 359, 377, 389, 426, 439, 454
HpyCH4V TGCA 6 cut(s) 12, 297, 338, 373, 769, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 712
HpyF3I CTNAG 2 cut(s) 108, 219
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 6 cut(s) 166, 247, 373, 682, 705, 1007
HspAI GCGC 2 cut(s) 364, 525
KasI GGCGCC 1 cut(s) 524
Kpn2I TCCGGA 1 cut(s) 533
Kzo9I GATC 3 cut(s) 138, 178, 911
LmnI GCTCC 5 cut(s) 124, 192, 283, 480, 764
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 4 cut(s) 136, 325, 702, 749
MaeIII GTNAC 3 cut(s) 90, 439, 454
MalI GATC 3 cut(s) 140, 180, 913
MboI GATC 3 cut(s) 138, 178, 911
MboII GAAGA 6 cut(s) 133, 211, 220, 280, 596, 960
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 2 cut(s) 189, 329
MluCI AATT 5 cut(s) 502, 723, 792, 954, 1009
Mly113I GGCGCC 1 cut(s) 525
MlyI GAGTC 4 cut(s) 218, 341, 557, 677
MmeI TCCRAC 3 cut(s) 874, 939, 996
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 533
MseI TTAA 2 cut(s) 500, 615
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 2 cut(s) 281, 534
MspR9I CCNGG 3 cut(s) 281, 288, 889
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 2 cut(s) 288, 889
MwoI GCNNNNNNNGC 2 cut(s) 370, 712
NarI GGCGCC 1 cut(s) 525
NciI CCSGG 1 cut(s) 281
NcoI CCATGG 1 cut(s) 243
NdeII GATC 3 cut(s) 138, 178, 911
NlaIII CATG 6 cut(s) 166, 247, 373, 682, 705, 1007
NlaIV GGNNCC 7 cut(s) 279, 285, 463, 526, 538, 760, 822
NmeAIII GCCGAG 1 cut(s) 905
NmuCI GTSAC 3 cut(s) 90, 439, 454
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 2 cut(s) 166, 373
PaeI GCATGC 1 cut(s) 166
PctI GAATGC 1 cut(s) 787
PfeI GAWTC 4 cut(s) 61, 407, 579, 884
PflFI GACNNNGTC 1 cut(s) 424
PkrI GCNGC 3 cut(s) 11, 296, 383
PleI GAGTC 4 cut(s) 217, 341, 557, 677
PluTI GGCGCC 1 cut(s) 528
PpsI GAGTC 4 cut(s) 217, 341, 557, 677
Psp6I CCWGG 2 cut(s) 286, 887
PspEI GGTNACC 1 cut(s) 90
PspFI CCCAGC 1 cut(s) 939
PspGI CCWGG 2 cut(s) 286, 887
PspN4I GGNNCC 7 cut(s) 279, 285, 463, 526, 538, 760, 822
PspPI GGNCC 2 cut(s) 133, 277
PstI CTGCAG 2 cut(s) 14, 299
PstNI CAGNNNCTG 1 cut(s) 136
PsuI RGATCY 1 cut(s) 911
PsyI GACNNNGTC 1 cut(s) 424
RsaI GTAC 3 cut(s) 16, 55, 356
RsaNI GTAC 3 cut(s) 15, 54, 355
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 2 cut(s) 500, 615
SatI GCNGC 3 cut(s) 10, 295, 382
Sau3AI GATC 3 cut(s) 138, 178, 911
Sau96I GGNCC 2 cut(s) 133, 277
ScaI AGTACT 3 cut(s) 16, 55, 356
SchI GAGTC 4 cut(s) 218, 341, 557, 677
ScrFI CCNGG 3 cut(s) 281, 288, 889
SduI GDGCHC 2 cut(s) 189, 329
SfaNI GCATC 4 cut(s) 136, 325, 702, 749
SfcI CTRYAG 2 cut(s) 10, 295
SfoI GGCGCC 1 cut(s) 526
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
SphI GCATGC 1 cut(s) 166
Sse9I AATT 5 cut(s) 502, 723, 792, 954, 1009
SsiI CCGC 2 cut(s) 30, 382
SspDI GGCGCC 1 cut(s) 524
StyD4I CCNGG 3 cut(s) 279, 286, 887
StyI CCWWGG 2 cut(s) 243, 520
TaaI ACNGT 7 cut(s) 103, 359, 377, 389, 426, 439, 454
TaqI TCGA 2 cut(s) 350, 719
TasI AATT 5 cut(s) 502, 723, 792, 954, 1009
TatI WGTACW 3 cut(s) 14, 53, 354
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 4 cut(s) 61, 407, 579, 884
Tru1I TTAA 2 cut(s) 500, 615
Tru9I TTAA 2 cut(s) 500, 615
TscAI CASTG 5 cut(s) 108, 116, 442, 463, 475
TseFI GTSAC 3 cut(s) 90, 439, 454
TseI GCWGC 2 cut(s) 9, 294
Tsp45I GTSAC 3 cut(s) 90, 439, 454
TspDTI ATGAA 2 cut(s) 17, 879
TspGWI ACGGA 1 cut(s) 751
TspRI CASTG 5 cut(s) 108, 116, 442, 463, 475
Tth111I GACNNNGTC 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 1 cut(s) 502
XceI RCATGY 2 cut(s) 166, 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
ZrmI AGTACT 3 cut(s) 16, 55, 356
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.