MD00G1001000.v1.1

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003697
Physical Location & Seq
Reverse (-)
238417 .. 244764
6348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1001000.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGGCCAAACCCCTCTTCTCCATCACCGTCTCCAAGACTGAAATGAGCGGGTCGTCGGTGGGCAATGAAGGTCACGCGCCGCTCTTCACAGTGGCGCAATGGGCTGAGATGGAGAATCAAGCTTTGATATTCAAGTATCTCAAGGCAGGACTCCCTGTTCCTTCTGAACTCCTCGCCCCTATTCGCACCAGTTTCAATCTCATTTCCCCCAATTTCTTACGCCACCACCCCACCTTGGCTTCCTATAGCCGGAAGAACATGGACCCGGAGCCAGGGCGGTGCCGGAGGACGGATGGCAAGAAATGGAGGTGCTCCAAAGATGCACATCCGGAGTCCAAGTATTGCGAGCGGCACATGAACCGAGGCCGTTATCGTTCAAGAAAGCTTGTGGAATCACAAACTTCCTCTCAATCTTTGTCGACTGTGACGTCAGACAGTGTGATTGGGATCTGCAGCCACAGTGGGAGCTTCCAGAGCATGCCCTTACACTCAATGGGAAATAGTGGAGCTCTATGCTTTGGAAGCAATGCATCTGAGTTGAAGATGGAGAGTATACCATATGGGGTATCTAACAGGGGAACCAGGGGCTGCAATGGATCAGAAGAACATAATTTCGTGTCAGAAGCTAGGAGTGCGAGGTGTCTTCGGGCAGACAGTAATGTAGATAATCCGTGGCTTCTAATGGCGCCTCAAGTTCCCTTGAAATTAGAACCGAGAAATAGTTCTCTTTGGCTAAACAACAGCTCTCATCTACAGACATTACAAAATATGGAGCCATTGAGCGTTAATGCTGAAATCACCAAACATGAGTATGTTGGTGAAGAGTTTGGCTTACCAGGATCTCAAAAACACGAACAGCATTCTCTTCAACCTTTCTTTGACGAGTGGCCTAAGTCAAAGGATCTGGGGTTCCATCAAAATGATCATAGATCAAACATAACTGAATTGTCAATGTCTAATGCAACGGTTCCTGCTAAATTTTTCCTAAGGAGCGCTGAATCACCCCGTGATGCCTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QLQ WRC

Protein Analysis

341

Amino Acids

37.99

Weight (kDa)

8.63

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 29 - 62 7.1e-13 QLQ
WRC PF08879 89 - 131 1.6e-19 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 427
AatII GACGTC 1 cut(s) 431
AccB1I GGYRCC 2 cut(s) 279, 685
AccBSI CCGCTC 3 cut(s) 48, 82, 349
AccI GTMKAC 2 cut(s) 419, 553
AccII CGCG 1 cut(s) 77
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 4 cut(s) 48, 80, 277, 349
AclWI GGATC 4 cut(s) 455, 604, 847, 909
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 977
AcyI GRCGYC 2 cut(s) 428, 686
AdeI CACNNNGTG 1 cut(s) 1007
AfeI AGCGCT 1 cut(s) 994
AfiI CCNNNNNNNGG 4 cut(s) 235, 249, 272, 289
AgsI TTSAA 6 cut(s) 133, 196, 378, 541, 703, 869
AjnI CCWGG 3 cut(s) 271, 581, 835
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AloI GAACNNNNNNTCC 4 cut(s) 141, 173, 893, 925
AluBI AGCT 6 cut(s) 122, 385, 468, 509, 626, 744
AluI AGCT 6 cut(s) 122, 385, 468, 509, 626, 744
Alw21I GWGCWC 2 cut(s) 314, 511
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 4 cut(s) 455, 604, 847, 909
AlwNI CAGNNNCTG 1 cut(s) 588
Aor13HI TCCGGA 1 cut(s) 328
Aor51HI AGCGCT 1 cut(s) 994
AoxI GGCC 3 cut(s) 3, 364, 887
ApeKI GCWGC 2 cut(s) 453, 588
ApoI RAATTY 1 cut(s) 977
Asp700I GAANNNNTTC 1 cut(s) 721
AspLEI GCGC 4 cut(s) 79, 97, 688, 995
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 4 cut(s) 16, 790, 830, 993
AvaII GGWCC 1 cut(s) 262
AxyI CCTNAGG 1 cut(s) 986
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 279, 685
BanII GRGCYC 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 635
Bbv12I GWGCWC 2 cut(s) 314, 511
BbvI GCAGC 2 cut(s) 465, 575
BccI CCATC 5 cut(s) 29, 103, 287, 538, 921
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 3 cut(s) 273, 583, 837
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 1 cut(s) 627
BfmI CTRYAG 3 cut(s) 244, 451, 752
BfoI RGCGCY 2 cut(s) 689, 996
BisI GCNGC 4 cut(s) 80, 350, 454, 589
BlsI GCNGC 4 cut(s) 81, 351, 455, 590
Bme1390I CCNGG 4 cut(s) 266, 273, 583, 837
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 8 cut(s) 264, 270, 281, 580, 687, 774, 911, 969
BmrFI CCNGG 4 cut(s) 266, 273, 583, 837
BmsI GCATC 3 cut(s) 310, 539, 1000
BpiI GAAGAC 1 cut(s) 635
BpuEI CTTGAG 2 cut(s) 125, 675
BpuMI CCSGG 1 cut(s) 266
BsaBI GATNNNNATC 2 cut(s) 324, 446
BsaHI GRCGYC 2 cut(s) 428, 686
BsaJI CCNNGG 5 cut(s) 234, 272, 361, 582, 671
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 249, 272, 289
Bse1I ACTGG 1 cut(s) 189
Bse21I CCTNAGG 1 cut(s) 986
Bse3DI GCAATG 4 cut(s) 70, 104, 532, 598
Bse8I GATNNNNATC 2 cut(s) 324, 446
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 3 cut(s) 273, 583, 837
BseDI CCNNGG 5 cut(s) 234, 272, 361, 582, 671
BseGI GGATG 2 cut(s) 298, 325
BseJI GATNNNNATC 2 cut(s) 324, 446
BseLI CCNNNNNNNGG 4 cut(s) 235, 249, 272, 289
BseMI GCAATG 4 cut(s) 70, 104, 532, 598
BseMII CTCAG 2 cut(s) 96, 525
BseNI ACTGG 1 cut(s) 189
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 2 cut(s) 465, 575
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 3 cut(s) 5, 366, 889
BshNI GGYRCC 2 cut(s) 279, 685
BsiHKAI GWGCWC 2 cut(s) 314, 511
BsiSI CCGG 4 cut(s) 250, 266, 283, 329
BslI CCNNNNNNNGG 4 cut(s) 235, 249, 272, 289
BsmAI GTCTC 1 cut(s) 34
BsmBI CGTCTC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 3 cut(s) 5, 366, 889
Bsp1286I GDGCHC 2 cut(s) 314, 511
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 6 cut(s) 447, 596, 839, 901, 922, 929
BspACI CCGC 4 cut(s) 48, 80, 277, 349
BspANI GGCC 3 cut(s) 5, 366, 889
BspCNI CTCAG 2 cut(s) 97, 526
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 8 cut(s) 264, 270, 281, 580, 687, 774, 911, 969
BspMAI CTGCAG 1 cut(s) 455
BspPI GGATC 4 cut(s) 455, 604, 847, 909
BspQI GCTCTTC 1 cut(s) 89
BspT107I GGYRCC 2 cut(s) 279, 685
BsrBI CCGCTC 3 cut(s) 48, 82, 349
BsrDI GCAATG 4 cut(s) 70, 104, 532, 598
BsrI ACTGG 1 cut(s) 189
BssECI CCNNGG 5 cut(s) 234, 272, 361, 582, 671
BssMI GATC 6 cut(s) 447, 596, 839, 901, 922, 929
BssNAI GTATAC 1 cut(s) 554
BssNI GRCGYC 2 cut(s) 428, 686
BssT1I CCWWGG 1 cut(s) 234
Bst1107I GTATAC 1 cut(s) 554
Bst2UI CCWGG 3 cut(s) 273, 583, 837
Bst4CI ACNGT 7 cut(s) 28, 91, 424, 437, 461, 656, 967
Bst6I CTCTTC 4 cut(s) 20, 89, 816, 870
BstACI GRCGYC 2 cut(s) 428, 686
BstC8I GCNNGC 2 cut(s) 347, 479
BstDEI CTNAG 4 cut(s) 105, 534, 891, 986
BstDSI CCRYGG 1 cut(s) 671
BstF5I GGATG 2 cut(s) 298, 325
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 2 cut(s) 689, 996
BstHHI GCGC 4 cut(s) 79, 97, 688, 995
BstKTI GATC 6 cut(s) 450, 599, 842, 904, 925, 932
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 6 cut(s) 447, 596, 839, 901, 922, 929
BstMWI GCNNNNNNNGC 4 cut(s) 101, 474, 522, 632
BstNI CCWGG 3 cut(s) 273, 583, 837
BstNSI RCATGY 1 cut(s) 481
BstSCI CCNGG 4 cut(s) 264, 271, 581, 835
BstSFI CTRYAG 3 cut(s) 244, 451, 752
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 465, 575
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 3 cut(s) 447, 839, 901
BstYI RGATCY 3 cut(s) 447, 839, 901
BstZ17I GTATAC 1 cut(s) 554
Bsu36I CCTNAGG 1 cut(s) 986
BsuRI GGCC 3 cut(s) 5, 366, 889
BtgI CCRYGG 1 cut(s) 671
BtsCI GGATG 2 cut(s) 298, 325
BtsIMutI CAGTG 3 cut(s) 96, 442, 466
Cac8I GCNNGC 2 cut(s) 347, 479
CaiI CAGNNNCTG 1 cut(s) 588
CfoI GCGC 4 cut(s) 79, 97, 688, 995
Cfr13I GGNCC 1 cut(s) 262
CviAII CATG 4 cut(s) 259, 355, 478, 806
DdeI CTNAG 4 cut(s) 105, 534, 891, 986
DinI GGCGCC 1 cut(s) 687
DpnI GATC 6 cut(s) 449, 598, 841, 903, 924, 931
DpnII GATC 6 cut(s) 447, 596, 839, 901, 922, 929
DraIII CACNNNGTG 1 cut(s) 1007
DrdI GACNNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 20, 89, 816, 870
EarI CTCTTC 4 cut(s) 20, 89, 816, 870
Ecl136II GAGCTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 262
Eco47III AGCGCT 1 cut(s) 994
Eco53kI GAGCTC 1 cut(s) 509
Eco81I CCTNAGG 1 cut(s) 986
EcoICRI GAGCTC 1 cut(s) 509
EcoRII CCWGG 3 cut(s) 271, 581, 835
EcoT14I CCWWGG 1 cut(s) 234
EcoT22I ATGCAT 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 511
EgeI GGCGCC 1 cut(s) 687
EheI GGCGCC 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 4 cut(s) 262, 358, 481, 809
FatI CATG 4 cut(s) 258, 354, 477, 805
FauI CCCGC 1 cut(s) 41
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 2 cut(s) 419, 553
Fnu4HI GCNGC 4 cut(s) 80, 350, 454, 589
FokI GGATG 2 cut(s) 305, 312
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 4 cut(s) 80, 350, 454, 589
FspBI CTAG 1 cut(s) 627
GlaI GCGC 4 cut(s) 78, 96, 687, 994
GluI GCNGC 4 cut(s) 80, 350, 454, 589
HaeII RGCGCY 2 cut(s) 689, 996
HaeIII GGCC 3 cut(s) 5, 366, 889
HapII CCGG 4 cut(s) 250, 266, 283, 329
HhaI GCGC 4 cut(s) 79, 97, 688, 995
Hin1I GRCGYC 2 cut(s) 428, 686
Hin1II CATG 4 cut(s) 262, 358, 481, 809
Hin6I GCGC 4 cut(s) 77, 95, 686, 993
HinP1I GCGC 4 cut(s) 77, 95, 686, 993
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HindIII AAGCTT 2 cut(s) 120, 383
HinfI GANTC 5 cut(s) 115, 150, 332, 392, 998
HpaII CCGG 4 cut(s) 250, 266, 283, 329
HphI GGTGA 4 cut(s) 16, 790, 830, 993
Hpy166II GTNNAC 2 cut(s) 420, 554
Hpy188I TCNGA 5 cut(s) 166, 433, 535, 601, 622
Hpy188III TCNNGA 3 cut(s) 329, 378, 472
Hpy8I GTNNAC 2 cut(s) 420, 554
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 2 cut(s) 62, 171
HpyCH4III ACNGT 7 cut(s) 28, 91, 424, 437, 461, 656, 967
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 5 cut(s) 323, 453, 530, 591, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 474, 522, 632
HpyF3I CTNAG 4 cut(s) 105, 534, 891, 986
HpySE526I ACGT 1 cut(s) 428
Hsp92I GRCGYC 2 cut(s) 428, 686
Hsp92II CATG 4 cut(s) 262, 358, 481, 809
HspAI GCGC 4 cut(s) 77, 95, 686, 993
KasI GGCGCC 1 cut(s) 685
Kpn2I TCCGGA 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 6 cut(s) 447, 596, 839, 901, 922, 929
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 6 cut(s) 268, 317, 465, 506, 772, 990
Lsp1109I GCAGC 2 cut(s) 465, 575
LweI GCATC 3 cut(s) 310, 539, 1000
MaeI CTAG 1 cut(s) 627
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 2 cut(s) 71, 424
MalI GATC 6 cut(s) 449, 598, 841, 903, 924, 931
MbiI CCGCTC 3 cut(s) 48, 82, 349
MboI GATC 6 cut(s) 447, 596, 839, 901, 922, 929
MboII GAAGA 8 cut(s) 7, 76, 265, 553, 614, 635, 833, 857
MflI RGATCY 3 cut(s) 447, 839, 901
MhlI GDGCHC 2 cut(s) 314, 511
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 211, 610, 704, 944, 977
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 686
MlyI GAGTC 2 cut(s) 144, 341
MnlI CCTC 8 cut(s) 23, 182, 279, 300, 356, 415, 630, 699
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 532
MroI TCCGGA 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 721
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 786
MslI CAYNNNNRTG 2 cut(s) 810, 918
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 250, 266, 283, 329
MspR9I CCNGG 4 cut(s) 266, 273, 583, 837
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 3 cut(s) 273, 583, 837
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 4 cut(s) 101, 474, 522, 632
NarI GGCGCC 1 cut(s) 686
NciI CCSGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 559
NdeII GATC 6 cut(s) 447, 596, 839, 901, 922, 929
NlaIII CATG 4 cut(s) 262, 358, 481, 809
NlaIV GGNNCC 8 cut(s) 264, 270, 281, 580, 687, 774, 911, 969
NmuCI GTSAC 2 cut(s) 71, 424
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 1 cut(s) 481
PaeI GCATGC 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 89
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PctI GAATGC 1 cut(s) 859
PdmI GAANNNNTTC 1 cut(s) 721
PfeI GAWTC 3 cut(s) 115, 392, 998
PkrI GCNGC 4 cut(s) 81, 351, 455, 590
PleI GAGTC 2 cut(s) 144, 340
PluTI GGCGCC 1 cut(s) 689
PpsI GAGTC 2 cut(s) 144, 340
Psp124BI GAGCTC 1 cut(s) 511
Psp6I CCWGG 3 cut(s) 271, 581, 835
PspGI CCWGG 3 cut(s) 271, 581, 835
PspN4I GGNNCC 8 cut(s) 264, 270, 281, 580, 687, 774, 911, 969
PspPI GGNCC 1 cut(s) 262
PstI CTGCAG 1 cut(s) 455
PstNI CAGNNNCTG 1 cut(s) 588
PsuI RGATCY 3 cut(s) 447, 839, 901
RseI CAYNNNNRTG 2 cut(s) 810, 918
SacI GAGCTC 1 cut(s) 511
SalI GTCGAC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 4 cut(s) 80, 350, 454, 589
Sau3AI GATC 6 cut(s) 447, 596, 839, 901, 922, 929
Sau96I GGNCC 1 cut(s) 262
SchI GAGTC 2 cut(s) 144, 341
ScrFI CCNGG 4 cut(s) 266, 273, 583, 837
SduI GDGCHC 2 cut(s) 314, 511
SfaNI GCATC 3 cut(s) 310, 539, 1000
SfcI CTRYAG 3 cut(s) 244, 451, 752
SfoI GGCGCC 1 cut(s) 687
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 2 cut(s) 810, 918
SmlI CTYRAG 2 cut(s) 140, 690
SmoI CTYRAG 2 cut(s) 140, 690
SphI GCATGC 1 cut(s) 481
Sse9I AATT 5 cut(s) 211, 610, 704, 944, 977
SsiI CCGC 4 cut(s) 48, 80, 277, 349
SspDI GGCGCC 1 cut(s) 685
SspMI CTAG 1 cut(s) 627
SstI GAGCTC 1 cut(s) 511
StyD4I CCNGG 4 cut(s) 264, 271, 581, 835
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 7 cut(s) 28, 91, 424, 437, 461, 656, 967
TaiI ACGT 1 cut(s) 431
TaqI TCGA 1 cut(s) 419
TasI AATT 5 cut(s) 211, 610, 704, 944, 977
TauI GCSGC 2 cut(s) 82, 352
TfiI GAWTC 3 cut(s) 115, 392, 998
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 3 cut(s) 96, 442, 466
TseFI GTSAC 2 cut(s) 71, 424
TseI GCWGC 2 cut(s) 453, 588
Tsp45I GTSAC 2 cut(s) 71, 424
TspDTI ATGAA 2 cut(s) 81, 371
TspGWI ACGGA 2 cut(s) 305, 660
TspRI CASTG 3 cut(s) 96, 442, 466
VpaK11BI GGWCC 1 cut(s) 262
XapI RAATTY 1 cut(s) 977
XceI RCATGY 1 cut(s) 481
XmiI GTMKAC 2 cut(s) 419, 553
XmnI GAANNNNTTC 1 cut(s) 721
XspI CTAG 1 cut(s) 627
ZraI GACGTC 1 cut(s) 429
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.