MD06G1014700.v1.1

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003697
Physical Location & Seq
Reverse (-)
1876356 .. 1881403
5048 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1014700.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGAGCATCAAGCTTTGATATTCAAGTATCTCAAGGCAGGAGTCCCTGTTCCTTCTGAGCTCCTTGCCCCTATTCGCACCAGTTTCAACCTCATTTCCCCCAATTTCTTACCTCACCACCCCACCTGGGGTTATGCTTCCTATTGCGGGAAGAAGATAGACCCGGAGCCGGGGCGGTGCCGGAGGACGGATGGCAAGAAATGGAGGTGCTCCAAAGATGCATATCAGGACTCCAAGTATTGCGAGCGGCACATGAACCGCGGCCGCAATCGTTCAAGAAAGCTTGTGGAATCACAAACTGCCTCCCAATCTTTGTCAACTGTGACGTCAGACAGTGTGATCGGGGGCTCCAGCCACAGTGGGAGCTTCCAGAGCATGCCCTTACACTCAATTGGAAATAGTGGAGGCCTATGCTTTGGAGGCAATGGATCCGAGTTGAAGATGGAGGCTATACCGTATGGGGTGTCTAACAGGGGAACCAGGGGGTGCAATACAGCTGGATCAGAAGAACACAATTTCATGTCAGAAGCTAAGAGGTGTGTTCAGGCGCACGGTACTGTAGACAGTCCGTGGCGTCTAATGGAGCCTCAAGTTCCCTTAAAATCAGAACTGAGAAATAGTTCTCTTTGGCTAAACAACAGCTCTCAGCTACAGACATTACAAACTATGGAGTCATTGAGTGATGCTGCTGCAATCACCAAACAAGAGTATGTTGGTGGAGAATTTGGCTTACCAGGATCTCAAAAACACGAACAAAATTCTCTTCAGCCTTTATTTGATGAGTGGCCTAAGTCGAGGGATCTGGGGTTCCATCTGAATGATCATAGATCAAACACGACTGAGCTGACAATGTCTAATGCAGGGGCCACCCCTAAATATTGCACAAGGAGTGCTGAGTCACCCAATGATGCCTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QLQ WRC

Protein Analysis

307

Amino Acids

33.82

Weight (kDa)

8.57

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 2 - 26 5.4e-08 QLQ
WRC PF08879 55 - 97 1.9e-20 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 329
AccB1I GGYRCC 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 247
AccI GTMKAC 1 cut(s) 561
AccII CGCG 1 cut(s) 261
AciI CCGC 6 cut(s) 147, 175, 247, 259, 261, 265
AclWI GGATC 5 cut(s) 423, 436, 508, 745, 807
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 722, 757
AcuI CTGAAG 1 cut(s) 749
AcyI GRCGYC 2 cut(s) 326, 574
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 6 cut(s) 127, 128, 147, 169, 170, 187
AgsI TTSAA 4 cut(s) 25, 88, 276, 439
AjnI CCWGG 3 cut(s) 125, 479, 733
AloI GAACNNNNNNTCC 4 cut(s) 33, 65, 791, 823
AluBI AGCT 9 cut(s) 14, 61, 283, 366, 497, 530, 642, 649, 844
AluI AGCT 9 cut(s) 14, 61, 283, 366, 497, 530, 642, 649, 844
Alw21I GWGCWC 2 cut(s) 63, 212
AlwI GGATC 5 cut(s) 423, 436, 508, 745, 807
AoxI GGCC 4 cut(s) 262, 406, 785, 864
ApeKI GCWGC 2 cut(s) 686, 689
ApoI RAATTY 2 cut(s) 722, 757
Asp700I GAANNNNTTC 1 cut(s) 619
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 1 cut(s) 864
AsuC2I CCSGG 2 cut(s) 164, 171
AsuHPI GGTGA 3 cut(s) 107, 688, 891
BamHI GGATCC 1 cut(s) 428
BanI GGYRCC 1 cut(s) 177
BanII GRGCYC 2 cut(s) 63, 350
Bbv12I GWGCWC 2 cut(s) 63, 212
BbvI GCAGC 2 cut(s) 673, 676
BccI CCATC 3 cut(s) 185, 436, 819
BciT130I CCWGG 3 cut(s) 127, 481, 735
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 2 cut(s) 164, 171
BfmI CTRYAG 2 cut(s) 558, 650
BisI GCNGC 5 cut(s) 248, 262, 265, 687, 690
BlsI GCNGC 5 cut(s) 249, 263, 266, 688, 691
Bme1390I CCNGG 5 cut(s) 127, 164, 171, 481, 735
BmgT120I GGNCC 1 cut(s) 864
BmiI GGNNCC 8 cut(s) 168, 179, 349, 430, 478, 585, 809, 865
BmrFI CCNGG 5 cut(s) 127, 164, 171, 481, 735
BmsI GCATC 4 cut(s) 16, 208, 673, 898
BpmI CTGGAG 1 cut(s) 334
BpuEI CTTGAG 2 cut(s) 17, 573
BpuMI CCSGG 2 cut(s) 164, 171
BsaBI GATNNNNATC 1 cut(s) 222
BsaHI GRCGYC 2 cut(s) 326, 574
BsaJI CCNNGG 5 cut(s) 126, 170, 259, 480, 569
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 6 cut(s) 127, 128, 147, 169, 170, 187
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 430
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 3 cut(s) 127, 481, 735
BseDI CCNNGG 5 cut(s) 126, 170, 259, 480, 569
BseGI GGATG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 6 cut(s) 127, 128, 147, 169, 170, 187
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 5 cut(s) 48, 602, 659, 831, 885
BseNI ACTGG 1 cut(s) 81
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 2 cut(s) 673, 676
Bsh1236I CGCG 1 cut(s) 261
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 4 cut(s) 264, 408, 787, 866
BshNI GGYRCC 1 cut(s) 177
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 63, 212
BsiSI CCGG 3 cut(s) 164, 170, 181
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 6 cut(s) 127, 128, 147, 169, 170, 187
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 4 cut(s) 264, 408, 787, 866
Bsp1286I GDGCHC 3 cut(s) 63, 212, 350
Bsp143I GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
BspACI CCGC 6 cut(s) 147, 175, 247, 259, 261, 265
BspANI GGCC 4 cut(s) 264, 408, 787, 866
BspCNI CTCAG 5 cut(s) 49, 603, 658, 832, 886
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 8 cut(s) 168, 179, 349, 430, 478, 585, 809, 865
BspPI GGATC 5 cut(s) 423, 436, 508, 745, 807
BspT107I GGYRCC 1 cut(s) 177
BsrBI CCGCTC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 430
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 5 cut(s) 126, 170, 259, 480, 569
BssMI GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
BssNI GRCGYC 2 cut(s) 326, 574
Bst2UI CCWGG 3 cut(s) 127, 481, 735
Bst4CI ACNGT 7 cut(s) 322, 335, 359, 456, 554, 559, 566
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 2 cut(s) 326, 574
BstC8I GCNNGC 2 cut(s) 245, 377
BstDEI CTNAG 7 cut(s) 57, 531, 611, 645, 789, 840, 894
BstDSI CCRYGG 2 cut(s) 259, 569
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 7 cut(s) 342, 431, 503, 740, 802, 823, 830
BstMBI GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 2 cut(s) 372, 420
BstNI CCWGG 3 cut(s) 127, 481, 735
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 5 cut(s) 125, 162, 169, 479, 733
BstSFI CTRYAG 2 cut(s) 558, 650
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 673, 676
BstX2I RGATCY 3 cut(s) 428, 737, 799
BstYI RGATCY 3 cut(s) 428, 737, 799
BstZI CGGCCG 1 cut(s) 262
BsuRI GGCC 4 cut(s) 264, 408, 787, 866
BtgI CCRYGG 2 cut(s) 259, 569
BtsCI GGATG 1 cut(s) 196
BtsIMutI CAGTG 2 cut(s) 340, 364
Cac8I GCNNGC 2 cut(s) 245, 377
CciNI GCGGCCGC 1 cut(s) 262
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 1 cut(s) 864
Cfr42I CCGCGG 1 cut(s) 262
CseI GACGC 1 cut(s) 563
Csp6I GTAC 1 cut(s) 555
CviAII CATG 3 cut(s) 253, 376, 520
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 7 cut(s) 57, 531, 611, 645, 789, 840, 894
DpnI GATC 7 cut(s) 341, 430, 502, 739, 801, 822, 829
DpnII GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Ecl136II GAGCTC 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 262
Eco147I AGGCCT 1 cut(s) 408
Eco24I GRGCYC 2 cut(s) 63, 350
Eco52I CGGCCG 1 cut(s) 262
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 749
EcoICRI GAGCTC 1 cut(s) 61
EcoRII CCWGG 3 cut(s) 125, 479, 733
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 63, 350
FaeI CATG 3 cut(s) 256, 379, 523
FaqI GGGAC 1 cut(s) 29
FatI CATG 3 cut(s) 252, 375, 519
FauI CCCGC 1 cut(s) 140
FbaI TGATCA 1 cut(s) 820
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 5 cut(s) 248, 262, 265, 687, 690
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 2 cut(s) 63, 350
Fsp4HI GCNGC 5 cut(s) 248, 262, 265, 687, 690
GlaI GCGC 1 cut(s) 549
GluI GCNGC 5 cut(s) 248, 262, 265, 687, 690
GsuI CTGGAG 1 cut(s) 334
HaeIII GGCC 4 cut(s) 264, 408, 787, 866
HapII CCGG 3 cut(s) 164, 170, 181
HgaI GACGC 1 cut(s) 563
HhaI GCGC 1 cut(s) 550
Hin1I GRCGYC 2 cut(s) 326, 574
Hin1II CATG 3 cut(s) 256, 379, 523
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 2 cut(s) 12, 281
HinfI GANTC 5 cut(s) 42, 230, 290, 671, 896
HpaII CCGG 3 cut(s) 164, 170, 181
HphI GGTGA 3 cut(s) 107, 688, 891
Hpy166II GTNNAC 2 cut(s) 318, 562
Hpy188I TCNGA 7 cut(s) 58, 331, 433, 505, 526, 607, 816
Hpy188III TCNNGA 3 cut(s) 227, 276, 370
Hpy8I GTNNAC 2 cut(s) 318, 562
HpyAV CCTTC 1 cut(s) 63
HpyCH4III ACNGT 7 cut(s) 322, 335, 359, 456, 554, 559, 566
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 5 cut(s) 221, 489, 692, 860, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 420
HpyF3I CTNAG 7 cut(s) 57, 531, 611, 645, 789, 840, 894
HpySE526I ACGT 1 cut(s) 326
Hsp92I GRCGYC 2 cut(s) 326, 574
Hsp92II CATG 3 cut(s) 256, 379, 523
HspAI GCGC 1 cut(s) 548
Ksp22I TGATCA 1 cut(s) 820
KspI CCGCGG 1 cut(s) 262
Kzo9I GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
LmnI GCTCC 7 cut(s) 4, 66, 166, 215, 353, 363, 583
Lsp1109I GCAGC 2 cut(s) 673, 676
LweI GCATC 4 cut(s) 16, 208, 673, 898
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 2 cut(s) 322, 897
MalI GATC 7 cut(s) 341, 430, 502, 739, 801, 822, 829
MbiI CCGCTC 1 cut(s) 247
MboI GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
MboII GAAGA 5 cut(s) 163, 166, 451, 518, 755
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 3 cut(s) 428, 737, 799
MhlI GDGCHC 3 cut(s) 63, 212, 350
MluCI AATT 5 cut(s) 103, 390, 514, 722, 757
MlyI GAGTC 4 cut(s) 51, 224, 680, 905
Mph1103I ATGCAT 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 619
MseI TTAA 1 cut(s) 599
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 2 cut(s) 261, 497
MspI CCGG 3 cut(s) 164, 170, 181
MspR9I CCNGG 5 cut(s) 127, 164, 171, 481, 735
MunI CAATTG 1 cut(s) 390
MvaI CCWGG 3 cut(s) 127, 481, 735
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 372, 420
NciI CCSGG 2 cut(s) 164, 171
NdeII GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
NlaIII CATG 3 cut(s) 256, 379, 523
NlaIV GGNNCC 8 cut(s) 168, 179, 349, 430, 478, 585, 809, 865
NmuCI GTSAC 2 cut(s) 322, 897
NotI GCGGCCGC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 1 cut(s) 379
PaeI GCATGC 1 cut(s) 379
PceI AGGCCT 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 619
PfeI GAWTC 1 cut(s) 290
PflFI GACNNNGTC 1 cut(s) 850
PkrI GCNGC 5 cut(s) 249, 263, 266, 688, 691
PleI GAGTC 4 cut(s) 50, 224, 679, 904
PpsI GAGTC 4 cut(s) 50, 224, 679, 904
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 3 cut(s) 125, 479, 733
PspGI CCWGG 3 cut(s) 125, 479, 733
PspN4I GGNNCC 8 cut(s) 168, 179, 349, 430, 478, 585, 809, 865
PspPI GGNCC 1 cut(s) 864
PsuI RGATCY 3 cut(s) 428, 737, 799
PsyI GACNNNGTC 1 cut(s) 850
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
RseI CAYNNNNRTG 1 cut(s) 816
SacI GAGCTC 1 cut(s) 63
SacII CCGCGG 1 cut(s) 262
SaqAI TTAA 1 cut(s) 599
SatI GCNGC 5 cut(s) 248, 262, 265, 687, 690
Sau3AI GATC 7 cut(s) 339, 428, 500, 737, 799, 820, 827
Sau96I GGNCC 1 cut(s) 864
SchI GAGTC 4 cut(s) 51, 224, 680, 905
ScrFI CCNGG 5 cut(s) 127, 164, 171, 481, 735
SduI GDGCHC 3 cut(s) 63, 212, 350
SfaNI GCATC 4 cut(s) 16, 208, 673, 898
SfcI CTRYAG 2 cut(s) 558, 650
Sfr303I CCGCGG 1 cut(s) 262
SgrBI CCGCGG 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 2 cut(s) 32, 588
SmoI CTYRAG 2 cut(s) 32, 588
SphI GCATGC 1 cut(s) 379
Sse9I AATT 5 cut(s) 103, 390, 514, 722, 757
SseBI AGGCCT 1 cut(s) 408
SsiI CCGC 6 cut(s) 147, 175, 247, 259, 261, 265
SspI AATATT 1 cut(s) 878
SstI GAGCTC 1 cut(s) 63
StuI AGGCCT 1 cut(s) 408
StyD4I CCNGG 5 cut(s) 125, 162, 169, 479, 733
TaaI ACNGT 7 cut(s) 322, 335, 359, 456, 554, 559, 566
TaiI ACGT 1 cut(s) 329
TaqI TCGA 1 cut(s) 794
TasI AATT 5 cut(s) 103, 390, 514, 722, 757
TauI GCSGC 3 cut(s) 250, 264, 267
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 599
Tru9I TTAA 1 cut(s) 599
TscAI CASTG 2 cut(s) 340, 364
TseFI GTSAC 2 cut(s) 322, 897
TseI GCWGC 2 cut(s) 686, 689
Tsp45I GTSAC 2 cut(s) 322, 897
TspDTI ATGAA 2 cut(s) 269, 508
TspGWI ACGGA 2 cut(s) 203, 558
TspRI CASTG 2 cut(s) 340, 364
Tth111I GACNNNGTC 1 cut(s) 850
XapI RAATTY 2 cut(s) 722, 757
XceI RCATGY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 561
XmnI GAANNNNTTC 1 cut(s) 619
ZraI GACGTC 1 cut(s) 327
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.