Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004607

Physical Location & Seq

Genomic Coordinates Forward (+)

6917252 .. 6929235

Gene Structure 11984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1040500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4995 bp

ATGGAAGCCGACGAGGAAGATGCTTCCAAGGTGAACGAGCCTTCGATTTCACTCCAAGACGACGCTTTACCGTTGCCTCATACAGGAGAGGAGCTCCGGAACACCGAACCACAATGCGACCCGGAGTCTGCACCTGAGCCCGAACATGGCGGCTCGGGGCTTGGGGTCGACGGAGAATCTGATTCGGTTGCGGCTTCGGTAGAGAGAGGCGATGGGGATGGAATTGAAGGAGGGTGTAAGGAGGGGGATACGGAAATGGAGGATGTGGCGGAGGCGGTGGTGGTGGCTGCGGAGACAGTTGAAACTGATGCAGTGGAGGATTTGGGGGTGGCGATGGAGAAGTCTGCTGCTGACGCGGGAGAGAAGATGGTGGTGGAAGAGGAGACGAATGTGGTAGAAGGTCACGTGGCCGAGGACAGGGAGGTTGAGGACGTGACGAATGTGGCTGAAAGTCACTTTAGAGAGGAAACGGAGGTCAAGGAGGAGATGCATGTAGCTGGAGGTCACGTGACAGAGGAAACTGAGGTGAAGGAGGAAATGACTGTGGCCGGTGGTCATGTGACAGAGGATACTGAGGTTGTGAAGGGGATGAATGTGGCTGAAGGTCATAGCACAGAGGAAGCTGAGGTTGGGGATGTGGATGATGAGGTATTGGAGACAGAGATGAAGGTGTTGCCTGAGGAAGCTGCTGTGGCTAACTCGGACATGCAGGAGGCTGATTCTAATGTGGCTGAAACGGCTGAGGAGACTGTGCTTTCAGTGAAGGATGAAACAGAGCAAATGACGGGTGAAGCCAAGGGAGGTGAGGTTGAGGCCGAGACAGTGGGAGAGGAGGATGGGAACGAGGCTGTGGGAGAGGAGGATGGGAACGAGGCTGTGGGAGAGGAGGATGGGAACGAGGCAGAGGAGGCTGAGGCCGAAGCGGAAGGAGAGGAGGCTGAAGCAGGGGGAGATGATGAGGAGAATGCGGCAGACATGTTGGCTGAGACTGACACAGTTGGGGAGGTGGATATGGTTGATGATGTAATGGAGGAGACAACGGAGGCAGAGGAGATGGGGGCAGCAGAGGAAGAAGCAGAAGAAATGGAGGTGGAAGAGGAGGAGGAGACGAAAAGGGCCGGCAGTGGCGGGAAGAGGAAGCATGTGAAGAGTTCTAAGGACACGGGAAAACTCCTCTCAAAGAAGAAAATGGAAGAGGATGTCTGTTTCATTTGCTTTGATGGGGGTGAGCTTGTGCTTTGTGACCGCAGGGGATGTCCCAAAGCATACCATCCTTCATGTGTTAATCGTGATGAGGCCTTCTTTCGAGCCAAGGGTCGATGGAATTGCGGTTGGCACCTTTGTAGCAGCTGTGAAAAGAGTGCCCACTACATGTGTTATACATGTACCTTTTCCTTGTGCAAGGCGTGTATCAAAGATGCTGTTATCTTATGTGTTCGAGGAAACAAAGGCTTCTGTGAGACATGCATGAAAACTATCATGATGATTGAAAAGAATGAGCAAGGAAACAAGAACAAGGATGAAGTCGATTTTGATGATAAAAGCAGCTGGGAGTACCTCTTCAAGGATTATTGGATAGACTTAAAAGAGAAGTTATCTCTAACGCTTGATGACCTTGCTCAGGCCAAGAATCCAAGGAAAGGATCAACTGGACGTGCTAATAAACAGGAGTCACGTGATGAACCCTATGATGCTAATGATGGTGGAGGGTCTGATTCTGACAACTCTGAGAATTTGGATTTAGTTAACCCTAAAAAAAGAAAGTCCAAAAAACGAATGAGAACCCGTGCAAAGGGGAGGGATTCATCAAGTGCAGCTACAGCAACCGGCTCTGGAGGTCCATCTGCAGATAATAGTGCAGGGTGGGCATCAAAGGAACTTCTAGAGTTTGTAATGCACATGAGAGATGGTGATGAGTCTCCTTTATCTCAGTTTGATGTCCAGGCTCTCTTGCTAGAATATATAAAAAGGAACAAACTTCGTGACCCTCGGCGAAAAAGTCAGATAGTTTGTGATATAAGGCTTCAAAATTTATTTGGGAAACCACGCGTTGGGCATTTTGAAATGTTAAAGCTTCTTGAATCCCACTTTCTTGTAAAAGAGGATGCTTACACTGATGATCTTCAAGGGAGTGTTGTTGATACCAATGACAATCAGTTGGAGGTTGATGGCAACTCTGATACCCCAGCAAAGGCCAGTAAAGATAAGAGACGTAAAGCACGTAAAAAGGGTGATGGGAAAGGACCTCAATCTAATATTGACGACTTTGCAGCCATTGATATGCATAACATCAATTTAATTTTCTTAAGGCGTAATTTAGTGGAAGATCTACTTGATGATCTGGATAACTTTCAAGACAAGGTTGCTGGCTCTTTTGTCAGAATTAGAATTTCTGGTAGTGGTCAAAAGCAAGATTTGTATAGGCTGGTGCAAATTATAGGTACATGCAAAGGTGCCGAGCCATATAAAGTTGGTAAAAGGATGACAGACATCTTGCTAGAGATATTAAACTTAAACAAGACAGAGATCATTTCGATTGATATCATCTCAAACCAAGAGTTCACTGAGGACGAATGCAAACGACTGCGTCAGAGCATAAAATGTGGACTTATCAACCGGCTTACTGTGGGTGATGTTCAAGACAAAGCAATAGCGCTCCAAGCAGTCAGAGTCAAAGATTGGTTAGAAACGGAGATAGTGAGACTCATTCATCTTCGTGATCGAGCTAGTGAAAAAGGGCGCAGGAAGGAATATCCTTTACTTTTCAAAGAGTGTGTAGAGAAGTTGCAGCTTCTAAAGACGCCTGAGGAACGCCAGCGTAGACTAGAGGAAACTCTAGAAATACATGCGGATCCAAATATGGACCCGAGTTATGAATCTGAGGAGGAGGAAGACGAAGGAGATGACAAGAGACAAGATAGTTACACGAGACCTACAGGTTTTTCCTTTGGAAGGAAAGGGAGGGATCCAATTTCTCCACGAAGAGGAGGATCTTCTTTTAATGATTCCTGGGGTGTGACAAAGAATTATTCCAATACAAATCGAGAGTTAAGCAGGAATACGTCAAATAAGGGATTCTACAACAAAGCAGAAAATACAACTGGAGCTGGTGAGAGAGTGAATGATTCATGGGGTCAAGGAAGAGACAGAGAAACGCAGCAAACAAGCCACTGGGAGAAGAAACAAATTATTTCAAGTTTAGAAACAGGTGTTAGGAGTACGCAATCTGTGGTACAATCTGAATCATCTCCTGGTGGCTCAGGAAATTCAGTGGCACATTTCACCACTGGCGCAGCACAATCGACAGCCCCTATCAATGATTCAGAAAAAATATGGCATTACAAGGATCCTTCCGGGAAGATTCAGGGACCATTTTCCATGGCACAGCTACGCAAATGGAATAACACTGGCTATTTTCCCGCCAACTTGAGAGTTTGGAAAAACACTGAGAAGGAAGAAGATTCTATCCTGGTAACTGATGTATTGGCTGGAAAGTTCCAGAAAGACCCTTCAGTGGTTGACAGTAGTTTTCTAAAGGCCCAAATGGTTCATGATTCATGTCTCTCACCTGCACCGTCTGGGAAGCCTCAAGGAGGTCTCTTTCAACGGGGTACAGAAGGCCAAGCTGGAGGAGGAAGTTGGGGATCAGAAAACGAAATTACTTCATCCTCAGCCAGAGGTACTCTATCGTCAGTTGAGGTTCCTAAGTATTCTTCAGATGGGTGGGGCACAACAAACTTTCCCTCGCCTACTCCATCCCAAACTCCATTAGGTGGGGCAAGGGGTCAAGCTTATGAAAGTAATTGGTCACCCACCCCTGTTCATCCAACTGGCTCTGTCTTGGGTGGAAATGGAGTGATGCAACCTAGTACAGTAGCTACTCCAGAAAGTGCACTGCGAGTTTCTGGAATTGATCGCTCAAGCTCTCTTTCTGGCATTAATGCAGTACCCAAGTCTGAAACTGCTGTGCTTCTGGGCTCTACAAATACTCGTCAAATGCATGGTCAAGTAAATGCTTCAATGAATCAAGTAACAGATGTGAAAAATCTTGTTTCAAATCTGCAAAATCTGGTACAGACTGTTACTAATCGTACTCCCGGTGGTGATACTCGAGGGTGGGCTTCTGGTTCAGTTCCAAAACAGGAAATGACTGCTCCAGGGCCAGTACCTGGGAGTGAAACTCAATCCTGGGCAGGTGCTCCGTCCCAGAGGATAGAACCAATTAATGCTTCTACTATGCCTGCACAACATCCGGCCCATGGTTACTGGGGTAACGCTTCATCTACTAACAATGCTACCCAGTCTATTAATACAGGAAATGCAGCTGGAAATTTGCCTTCATCAGGCTTCTCTGGTGTTCCACAGTCTGATCCTTGGAGACCCCAAGTCCCAGCTAATCAATCATACATTCAGCCTCCAGCACCATCGCCCCAGGTACCTTGGAGCATGGGTGTCCCAGATAACCAAAGTTCTCTGCCAAGAACGGGGCAAGAGAATCAAAACGCAGGTTGGGCACCAATTGGGGGAAATCCAAACATGGCCTGGAGAGGACAAGTGCCAGGAAATACAAACATGAACTGGGGAGCTCCGGGACAGGGTCCAGGGTGGGCTGGACCTGGTCAAGGTCCAGTACCTGGAAATGCAGCGCCTAATTGGGTCCAACCTGCTGCTCAAGGACCTACTTCGTTAAGTGGAAATCCCGGTTGGGCTCCATCTGGCCAAGGGCCAGCAGTTGCGAACACCAATCCTGGTTGGGGTGGGCAAACTCAAAATGGAGGAGATAGGTTCTCAAATCAAAGGGATCGTGCTCCGCATGGCGGAGATTCTGGTTATGGTGGGGGCCAGCCCTGGAATAGGCAGTCGTCCTTTGGTGGTGGCGGCGGGGGAGGAGGGTCGTCGAGGCCGCCCTTCAAAGGGCCCAGAGTGTGCAGGTTCTTTGAGAGTGGTCACTGCAAGAAGGGCGCAGCGTGTGATTATCTGCACCCGGACCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010964 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031935 GO:0032259 GO:0032776 GO:0033043 GO:0033044 GO:0034641 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0044728 GO:0046483 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051252 GO:0060255 GO:0060966 GO:0060968 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:1901360 GO:1902275 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GYF Plus-3 SWIB zf-CCCH

Protein Analysis

1665

Amino Acids

180.1

Weight (kDa)

4.73

Isoelectric Point (pI)

49.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SWIB	PF02201	624 - 697	9.6e-15	SWIB/MDM2 domain
Plus-3	PF03126	763 - 866	7.8e-24	Plus-3 domain
NERD_plant	PF25980	888 - 941	1.5e-25	Plant NERD domain
GYF	PF02213	1112 - 1152	3.5e-15	GYF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004607)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16485 AT2G16485
fragaria_vesca	FvH4_2g10320 FvH4_2g10320 FvH4_2g10320 FvH4_2g10320 FvH4_2g10320 FvH4_2g10320 FvH4_2g10320
malus_domestica	MD00G1040500.v1.1 MD10G1064000.v1.1
prunus_persica	Prupe.8G085700_v2.0.a1
pyrus_communis	pycom05g04690 pycom05g04700 pycom10g04950 pycom10g04960
rosa_chinensis	RchiOBHm_Chr6g0267521
rosa_laevigata	RLG00000013994
rosa_multiflora	Rmu_co8013812.1_g000001 Rmu_co8150174.1_g000001 Rmu_sc0008030.1_g000001 Rmu_sc0012005.1_g000009
rosa_roxburghii	Rroxscaffold_7G00200660
rosa_rugosa	Rorug06G0033200 Rorug06G0033300 Rorug06G0033400
rosa_samantha	Rh6AG154800 Rh6BG155500 Rh6CG150700 Rh6DG140700
rosa_wichuraiana	Rw6G013290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	3577, 4184
Acc36I	ACCTGC	5 cut(s)	3577, 4184, 4496, 4672, 4920
Acc65I	GGTACC	1 cut(s)	4435
AccB1I	GGYRCC	4 cut(s)	1335, 2453, 4435, 4513
AccB7I	CCANNNNNTGG	4 cut(s)	2051, 3773, 4169, 4634
AccI	GTMKAC	2 cut(s)	168, 2821
AccII	CGCG	2 cut(s)	356, 2049
AccIII	TCCGGA	1 cut(s)	96
AcoI	YGGCCR	3 cut(s)	408, 546, 4717
AcsI	RAATTY	5 cut(s)	1732, 2029, 2388, 3265, 4330
AcuI	CTGAAG	4 cut(s)	621, 960, 3495, 3699
AcvI	CACGTG	3 cut(s)	406, 508, 1676
AcyI	GRCGYC	1 cut(s)	2801
AfeI	AGCGCT	1 cut(s)	2655
AflII	CTTAAG	1 cut(s)	2305
AflIII	ACRYGT	4 cut(s)	975, 1371, 1382, 2047
AhdI	GACNNNNNGTC	2 cut(s)	124, 4385
AjiI	CACGTC	2 cut(s)	433, 1655
AjuI	GAANNNNNNNTTGG	6 cut(s)	612, 644, 4213, 4245, 4687, 4719
Alw21I	GWGCWC	5 cut(s)	96, 3895, 4201, 4588, 4810
Alw44I	GTGCAC	1 cut(s)	3891
AlwNI	CAGNNNCTG	2 cut(s)	4169, 4634
Ama87I	CYCGRG	3 cut(s)	154, 2866, 4110
Aor13HI	TCCGGA	1 cut(s)	96
Aor51HI	AGCGCT	1 cut(s)	2655
ApaI	GGGCCC	1 cut(s)	4920
ApaLI	GTGCAC	1 cut(s)	3891
ApoI	RAATTY	5 cut(s)	1732, 2029, 2388, 3265, 4330
ArsI	GACNNNNNNTTYG	2 cut(s)	3988, 4020
AseI	ATTAAT	3 cut(s)	3939, 4224, 4308
Asp718I	GGTACC	1 cut(s)	4435
AspLEI	GCGC	5 cut(s)	2656, 2742, 3293, 4648, 4964
AsuC2I	CCSGG	6 cut(s)	122, 3355, 4098, 4590, 4701, 4985
AvaI	CYCGRG	3 cut(s)	154, 2866, 4110
AxyI	CCTNAGG	2 cut(s)	678, 2805
BaeGI	GKGCMC	5 cut(s)	1366, 3731, 3895, 4516, 4920
BaeI	ACNNNNGTAYC	2 cut(s)	561, 594
BalI	TGGCCA	1 cut(s)	4719
BamHI	GGATCC	3 cut(s)	2851, 2965, 3346
BanI	GGYRCC	4 cut(s)	1335, 2453, 4435, 4513
BanII	GRGCYC	6 cut(s)	96, 141, 3980, 4588, 4711, 4920
BauI	CACGAG	1 cut(s)	2926
BbrPI	CACGTG	3 cut(s)	406, 508, 1676
BbsI	GAAGAC	1 cut(s)	2898
Bbv12I	GWGCWC	5 cut(s)	96, 3895, 4201, 4588, 4810
BbvCI	CCTCAGC	4 cut(s)	624, 741, 912, 3670
BceAI	ACGGC	1 cut(s)	753
BcgI	CGANNNNNNTGC	5 cut(s)	36, 1308, 1342, 3282, 3316
BciVI	GTATCC	2 cut(s)	241, 562
BcnI	CCSGG	6 cut(s)	122, 3355, 4098, 4590, 4701, 4985
BfaI	CTAG	7 cut(s)	1883, 1955, 2498, 2727, 2825, 2837, 3867
BfmI	CTRYAG	3 cut(s)	1818, 1845, 2934
BfoI	RGCGCY	2 cut(s)	2657, 4649
BfrI	CTTAAG	1 cut(s)	2305
BfuAI	ACCTGC	5 cut(s)	3577, 4184, 4496, 4672, 4920
BfuI	GTATCC	2 cut(s)	241, 562
BglII	AGATCT	1 cut(s)	2326
BmeRI	GACNNNNNGTC	2 cut(s)	124, 4385
BmeT110I	CYCGRG	3 cut(s)	154, 2866, 4110
BmgBI	CACGTC	2 cut(s)	433, 1655
BmrI	ACTGGG	4 cut(s)	3181, 4276, 4294, 4588
BmsI	GCATC	8 cut(s)	10, 298, 477, 1408, 1681, 1877, 2095, 3849
BmuI	ACTGGG	4 cut(s)	3181, 4276, 4294, 4588
BpiI	GAAGAC	1 cut(s)	2898
BplI	GAGNNNNNCTC	4 cut(s)	78, 110, 4165, 4197
BpmI	CTGGAG	8 cut(s)	519, 1854, 3123, 3648, 3867, 4140, 4401, 4564
Bpu10I	CCTNAGC	7 cut(s)	135, 624, 741, 912, 1620, 3259, 3670
BpuEI	CTTGAG	4 cut(s)	3448, 3573, 3904, 4656
BpuMI	CCSGG	6 cut(s)	122, 3355, 4098, 4590, 4701, 4985
BsaAI	YACGTR	4 cut(s)	406, 508, 1676, 2222
BsaBI	GATNNNNATC	1 cut(s)	2540
BsaHI	GRCGYC	1 cut(s)	2801
BsaI	GGTCTC	3 cut(s)	2923, 3602, 4372
BsaWI	WCCGGW	1 cut(s)	96
BsaXI	ACNNNNNCTCC	6 cut(s)	492, 522, 792, 822, 4435, 4465
Bse118I	RCCGGY	4 cut(s)	548, 1118, 1826, 2616
Bse21I	CCTNAGG	2 cut(s)	678, 2805
Bse8I	GATNNNNATC	1 cut(s)	2540
BseAI	TCCGGA	1 cut(s)	96
BseJI	GATNNNNATC	1 cut(s)	2540
BseSI	GKGCMC	5 cut(s)	1366, 3731, 3895, 4516, 4920
BseYI	CCCAGC	3 cut(s)	1548, 2185, 4390
BsgI	GTGCAG	7 cut(s)	114, 1833, 1878, 3555, 4227, 4948, 4964
Bsh1236I	CGCG	2 cut(s)	356, 2049
BshNI	GGYRCC	4 cut(s)	1335, 2453, 4435, 4513
BsiHKAI	GWGCWC	5 cut(s)	96, 3895, 4201, 4588, 4810
BsiHKCI	CYCGRG	3 cut(s)	154, 2866, 4110
BslFI	GGGAC	6 cut(s)	1242, 3381, 4189, 4373, 4439, 4605
BsmBI	CGTCTC	3 cut(s)	377, 1100, 2203
BsmFI	GGGAC	6 cut(s)	1242, 3381, 4189, 4373, 4439, 4605
BsmI	GAATGC	2 cut(s)	970, 2579
Bso31I	GGTCTC	3 cut(s)	2923, 3602, 4372
BsoBI	CYCGRG	3 cut(s)	154, 2866, 4110
Bsp120I	GGGCCC	1 cut(s)	4916
Bsp13I	TCCGGA	1 cut(s)	96
Bsp19I	CCATGG	2 cut(s)	3378, 4258
BspEI	TCCGGA	1 cut(s)	96
BspFNI	CGCG	2 cut(s)	356, 2049
BspHI	TCATGA	2 cut(s)	1479, 3550
BspMAI	CTGCAG	1 cut(s)	1849
BspMI	ACCTGC	5 cut(s)	3577, 4184, 4496, 4672, 4920
BspT107I	GGYRCC	4 cut(s)	1335, 2453, 4435, 4513
BspTI	CTTAAG	1 cut(s)	2305
BspTNI	GGTCTC	3 cut(s)	2923, 3602, 4372
BsrFI	RCCGGY	4 cut(s)	548, 1118, 1826, 2616
BssAI	RCCGGY	4 cut(s)	548, 1118, 1826, 2616
BssNI	GRCGYC	1 cut(s)	2801
BssSI	CACGAG	1 cut(s)	2926
BssT1I	CCWWGG	9 cut(s)	27, 795, 1311, 1634, 3378, 4258, 4373, 4439, 4720
Bst2BI	CACGAG	1 cut(s)	2926
Bst6I	CTCTTC	8 cut(s)	372, 1089, 1127, 1142, 1188, 1565, 2977, 3136
BstACI	GRCGYC	1 cut(s)	2801
BstAFI	CTTAAG	1 cut(s)	2305
BstBAI	YACGTR	4 cut(s)	406, 508, 1676, 2222
BstC8I	GCNNGC	6 cut(s)	1120, 2368, 2816, 4242, 4728, 4844
BstDSI	CCRYGG	2 cut(s)	3378, 4258
BstEII	GGTNACC	1 cut(s)	3807
BstENI	CCTNNNNNAGG	8 cut(s)	81, 1562, 1619, 2950, 3591, 4341, 4620, 4852
BstFNI	CGCG	2 cut(s)	356, 2049
BstH2I	RGCGCY	2 cut(s)	2657, 4649
BstHHI	GCGC	5 cut(s)	2656, 2742, 3293, 4648, 4964
BstMWI	GCNNNNNNNGC	9 cut(s)	353, 692, 737, 908, 1820, 1865, 2660, 4511, 4959
BstNSI	RCATGY	9 cut(s)	494, 709, 979, 1145, 1375, 1386, 1467, 2448, 2849
BstPI	GGTNACC	1 cut(s)	3807
BstSFI	CTRYAG	3 cut(s)	1818, 1845, 2934
BstSLI	GKGCMC	5 cut(s)	1366, 3731, 3895, 4516, 4920
BstUI	CGCG	2 cut(s)	356, 2049
BstV2I	GAAGAC	1 cut(s)	2898
BstX2I	RGATCY	5 cut(s)	2326, 2851, 2965, 2990, 3346
BstYI	RGATCY	5 cut(s)	2326, 2851, 2965, 2990, 3346
Bsu36I	CCTNAGG	2 cut(s)	678, 2805
BsuI	GTATCC	2 cut(s)	241, 562
BtgI	CCRYGG	2 cut(s)	3378, 4258
BtgZI	GCGATG	3 cut(s)	225, 347, 4410
BtrI	CACGTC	2 cut(s)	433, 1655
BtsI	GCAGTG	4 cut(s)	318, 1129, 3893, 4948
BveI	ACCTGC	5 cut(s)	3577, 4184, 4496, 4672, 4920
Cac8I	GCNNGC	6 cut(s)	1120, 2368, 2816, 4242, 4728, 4844
CaiI	CAGNNNCTG	2 cut(s)	4169, 4634
CciI	TCATGA	2 cut(s)	1479, 3550
CfoI	GCGC	5 cut(s)	2656, 2742, 3293, 4648, 4964
Cfr10I	RCCGGY	4 cut(s)	548, 1118, 1826, 2616
CseI	GACGC	4 cut(s)	71, 362, 2576, 2809
CsiI	ACCWGGT	1 cut(s)	4615
DriI	GACNNNNNGTC	2 cut(s)	124, 4385
EaeI	YGGCCR	3 cut(s)	408, 546, 4717
Eam1104I	CTCTTC	8 cut(s)	372, 1089, 1127, 1142, 1188, 1565, 2977, 3136
Eam1105I	GACNNNNNGTC	2 cut(s)	124, 4385
EarI	CTCTTC	8 cut(s)	372, 1089, 1127, 1142, 1188, 1565, 2977, 3136
EciI	GGCGGA	2 cut(s)	284, 4833
Ecl136II	GAGCTC	2 cut(s)	94, 4586
Eco130I	CCWWGG	9 cut(s)	27, 795, 1311, 1634, 3378, 4258, 4373, 4439, 4720
Eco147I	AGGCCT	1 cut(s)	1298
Eco24I	GRGCYC	6 cut(s)	96, 141, 3980, 4588, 4711, 4920
Eco31I	GGTCTC	3 cut(s)	2923, 3602, 4372
Eco32I	GATATC	1 cut(s)	2542
Eco47III	AGCGCT	1 cut(s)	2655
Eco53kI	GAGCTC	2 cut(s)	94, 4586
Eco57I	CTGAAG	4 cut(s)	621, 960, 3495, 3699
Eco72I	CACGTG	3 cut(s)	406, 508, 1676
Eco81I	CCTNAGG	2 cut(s)	678, 2805
Eco88I	CYCGRG	3 cut(s)	154, 2866, 4110
Eco91I	GGTNACC	1 cut(s)	3807
EcoICRI	GAGCTC	2 cut(s)	94, 4586
EcoNI	CCTNNNNNAGG	8 cut(s)	81, 1562, 1619, 2950, 3591, 4341, 4620, 4852
EcoO109I	RGGNCCY	3 cut(s)	2243, 4676, 4916
EcoO65I	GGTNACC	1 cut(s)	3807
EcoRV	GATATC	1 cut(s)	2542
EcoT14I	CCWWGG	9 cut(s)	27, 795, 1311, 1634, 3378, 4258, 4373, 4439, 4720
EcoT22I	ATGCAT	4 cut(s)	492, 1469, 2286, 4002
EcoT38I	GRGCYC	6 cut(s)	96, 141, 3980, 4588, 4711, 4920
ErhI	CCWWGG	9 cut(s)	27, 795, 1311, 1634, 3378, 4258, 4373, 4439, 4720
Esp3I	CGTCTC	3 cut(s)	377, 1100, 2203
FalI	AAGNNNNNCTT	2 cut(s)	2316, 2348
FaqI	GGGAC	6 cut(s)	1242, 3381, 4189, 4373, 4439, 4605
FauI	CCCGC	4 cut(s)	349, 1121, 3427, 4874
FblI	GTMKAC	2 cut(s)	168, 2821
FriOI	GRGCYC	6 cut(s)	96, 141, 3980, 4588, 4711, 4920
FspBI	CTAG	7 cut(s)	1883, 1955, 2498, 2727, 2825, 2837, 3867
GlaI	GCGC	5 cut(s)	2655, 2741, 3292, 4647, 4963
GsaI	CCCAGC	3 cut(s)	1552, 2189, 4394
GsuI	CTGGAG	8 cut(s)	519, 1854, 3123, 3648, 3867, 4140, 4401, 4564
HaeII	RGCGCY	2 cut(s)	2657, 4649
HgaI	GACGC	4 cut(s)	71, 362, 2576, 2809
HhaI	GCGC	5 cut(s)	2656, 2742, 3293, 4648, 4964
Hin1I	GRCGYC	1 cut(s)	2801
Hin6I	GCGC	5 cut(s)	2654, 2740, 3291, 4646, 4962
HinP1I	GCGC	5 cut(s)	2654, 2740, 3291, 4646, 4962
HincII	GTYRAC	3 cut(s)	169, 1747, 3520
HindII	GTYRAC	3 cut(s)	169, 1747, 3520
HindIII	AAGCTT	2 cut(s)	2072, 3789
HpaI	GTTAAC	1 cut(s)	1747
Hpy166II	GTNNAC	8 cut(s)	34, 169, 1747, 2562, 2606, 2822, 3520, 3893
Hpy8I	GTNNAC	8 cut(s)	34, 169, 1747, 2562, 2606, 2822, 3520, 3893
Hpy99I	CGWCG	4 cut(s)	14, 65, 173, 4900
HpyCH4IV	ACGT	8 cut(s)	405, 432, 507, 1654, 1675, 2212, 2221, 3062
HpyF10VI	GCNNNNNNNGC	9 cut(s)	353, 692, 737, 908, 1820, 1865, 2660, 4511, 4959
HpySE526I	ACGT	8 cut(s)	405, 432, 507, 1654, 1675, 2212, 2221, 3062
Hsp92I	GRCGYC	1 cut(s)	2801
HspAI	GCGC	5 cut(s)	2654, 2740, 3291, 4646, 4962
Kpn2I	TCCGGA	1 cut(s)	96
KpnI	GGTACC	1 cut(s)	4439
KroI	GCCGGC	1 cut(s)	1118
KroNI	GCCGGC	1 cut(s)	1120
KspAI	GTTAAC	1 cut(s)	1747
LweI	GCATC	8 cut(s)	10, 298, 477, 1408, 1681, 1877, 2095, 3849
MabI	ACCWGGT	1 cut(s)	4615
MaeI	CTAG	7 cut(s)	1883, 1955, 2498, 2727, 2825, 2837, 3867
MaeII	ACGT	8 cut(s)	405, 432, 507, 1654, 1675, 2212, 2221, 3062
MfeI	CAATTG	1 cut(s)	4518
MflI	RGATCY	5 cut(s)	2326, 2851, 2965, 2990, 3346
MlsI	TGGCCA	1 cut(s)	4719
MluI	ACGCGT	1 cut(s)	2047
MluNI	TGGCCA	1 cut(s)	4719
MlyI	GAGTC	5 cut(s)	134, 1679, 1925, 2679, 2697
MmeI	TCCRAC	3 cut(s)	2139, 3851, 4684
Mox20I	TGGCCA	1 cut(s)	4719
Mph1103I	ATGCAT	4 cut(s)	492, 1469, 2286, 4002
MroI	TCCGGA	1 cut(s)	96
MroNI	GCCGGC	1 cut(s)	1118
MscI	TGGCCA	1 cut(s)	4719
MslI	CAYNNNNRTG	2 cut(s)	2279, 2715
Msp20I	TGGCCA	1 cut(s)	4719
MspA1I	CMGCKG	3 cut(s)	1350, 1548, 4325
MspCI	CTTAAG	1 cut(s)	2305
MunI	CAATTG	1 cut(s)	4518
Mva1269I	GAATGC	2 cut(s)	970, 2579
MvnI	CGCG	2 cut(s)	356, 2049
MwoI	GCNNNNNNNGC	9 cut(s)	353, 692, 737, 908, 1820, 1865, 2660, 4511, 4959
NaeI	GCCGGC	1 cut(s)	1120
NciI	CCSGG	6 cut(s)	122, 3355, 4098, 4590, 4701, 4985
NcoI	CCATGG	2 cut(s)	3378, 4258
NgoMIV	GCCGGC	1 cut(s)	1118
NmeAIII	GCCGAG	4 cut(s)	436, 841, 1969, 2482
NsiI	ATGCAT	4 cut(s)	492, 1469, 2286, 4002
NspI	RCATGY	9 cut(s)	494, 709, 979, 1145, 1375, 1386, 1467, 2448, 2849
PaeR7I	CTCGAG	1 cut(s)	4110
PagI	TCATGA	2 cut(s)	1479, 3550
PaqCI	CACCTGC	2 cut(s)	3577, 4184
PceI	AGGCCT	1 cut(s)	1298
PciI	ACATGT	3 cut(s)	975, 1371, 1382
PcsI	WCGNNNNNNNCGW	1 cut(s)	2218
PctI	GAATGC	2 cut(s)	970, 2579
PdiI	GCCGGC	1 cut(s)	1120
PflFI	GACNNNGTC	3 cut(s)	2586, 4596, 4617
PflMI	CCANNNNNTGG	4 cut(s)	2051, 3773, 4169, 4634
PfoI	TCCNGGA	2 cut(s)	3353, 4588
PleI	GAGTC	5 cut(s)	133, 1678, 1924, 2678, 2697
PmaCI	CACGTG	3 cut(s)	406, 508, 1676
PmlI	CACGTG	3 cut(s)	406, 508, 1676
PpsI	GAGTC	5 cut(s)	133, 1678, 1924, 2678, 2697
Ppu21I	YACGTR	4 cut(s)	406, 508, 1676, 2222
PpuMI	RGGWCCY	2 cut(s)	2243, 4676
PscI	ACATGT	3 cut(s)	975, 1371, 1382
PshBI	ATTAAT	3 cut(s)	3939, 4224, 4308
Psp124BI	GAGCTC	2 cut(s)	96, 4588
Psp5II	RGGWCCY	2 cut(s)	2243, 4676
PspCI	CACGTG	3 cut(s)	406, 508, 1676
PspEI	GGTNACC	1 cut(s)	3807
PspFI	CCCAGC	3 cut(s)	1548, 2185, 4390
PspOMI	GGGCCC	1 cut(s)	4916
PspPPI	RGGWCCY	2 cut(s)	2243, 4676
PspXI	VCTCGAGB	1 cut(s)	4110
PstI	CTGCAG	1 cut(s)	1849
PstNI	CAGNNNCTG	2 cut(s)	4169, 4634
PsuI	RGATCY	5 cut(s)	2326, 2851, 2965, 2990, 3346
PsyI	GACNNNGTC	3 cut(s)	2586, 4596, 4617
PvuII	CAGCTG	3 cut(s)	1350, 1548, 4325
RseI	CAYNNNNRTG	2 cut(s)	2279, 2715
SacI	GAGCTC	2 cut(s)	96, 4588
SalI	GTCGAC	1 cut(s)	167
SchI	GAGTC	5 cut(s)	134, 1679, 1925, 2679, 2697
SexAI	ACCWGGT	1 cut(s)	4615
SfaNI	GCATC	8 cut(s)	10, 298, 477, 1408, 1681, 1877, 2095, 3849
SfcI	CTRYAG	3 cut(s)	1818, 1845, 2934
Sfr274I	CTCGAG	1 cut(s)	4110
SlaI	CTCGAG	1 cut(s)	4110
SmiMI	CAYNNNNRTG	2 cut(s)	2279, 2715
SmlI	CTYRAG	6 cut(s)	2305, 3427, 3588, 3919, 4110, 4671
SmoI	CTYRAG	6 cut(s)	2305, 3427, 3588, 3919, 4110, 4671
SseBI	AGGCCT	1 cut(s)	1298
SspI	AATATT	1 cut(s)	2257
SspMI	CTAG	7 cut(s)	1883, 1955, 2498, 2727, 2825, 2837, 3867
SstI	GAGCTC	2 cut(s)	96, 4588
StuI	AGGCCT	1 cut(s)	1298
StyI	CCWWGG	9 cut(s)	27, 795, 1311, 1634, 3378, 4258, 4373, 4439, 4720
TaiI	ACGT	8 cut(s)	408, 435, 510, 1657, 1678, 2215, 2224, 3065
TatI	WGTACW	1 cut(s)	3869
TauI	GCSGC	5 cut(s)	153, 194, 971, 4881, 4906
TspGWI	ACGGA	6 cut(s)	186, 266, 485, 1055, 2705, 4191
Tth111I	GACNNNGTC	3 cut(s)	2586, 4596, 4617
Van91I	CCANNNNNTGG	4 cut(s)	2051, 3773, 4169, 4634
Vha464I	CTTAAG	1 cut(s)	2305
VneI	GTGCAC	1 cut(s)	3891
VspI	ATTAAT	3 cut(s)	3939, 4224, 4308
XagI	CCTNNNNNAGG	8 cut(s)	81, 1562, 1619, 2950, 3591, 4341, 4620, 4852
XapI	RAATTY	5 cut(s)	1732, 2029, 2388, 3265, 4330
XbaI	TCTAGA	2 cut(s)	1882, 2836
XceI	RCATGY	9 cut(s)	494, 709, 979, 1145, 1375, 1386, 1467, 2448, 2849
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4539
XhoI	CTCGAG	1 cut(s)	4110
XmiI	GTMKAC	2 cut(s)	168, 2821
XspI	CTAG	7 cut(s)	1883, 1955, 2498, 2727, 2825, 2837, 3867
Zsp2I	ATGCAT	4 cut(s)	492, 1469, 2286, 4002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.