Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000767

Physical Location & Seq

Genomic Coordinates Forward (+)

15326984 .. 15329876

Gene Structure 2893 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1075800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGTCAGGGGCAAAGCCGGCCGCGGCAGAGCATGTCGGCTTCGCCAACGTGGATGCGAACAAGTCCACTGCCACCGCCAAAGCCGAAGGATGTTGCAATCCGGTCAAGAAAAGCGGACCCATTTCGATGGATCACGTTCTTCTAGCCTTGCGCGAGACCAAGGAGGACAGGGACGTCCGAATTCGAAGCCTTTTCAATTTCTTCGATGCTGAGGATCTTGGGTATTTGGATTATTCTCAGATCGAGGCCGGATTGTCGGCCCTTCAGATTCCGCCTGAATACAAGTACGCCAATGATCTTCTGAAGGTCTGTGATGCTAACAGGGATGGAAGGGTCGATTATCTTGAGTTCCGGCGGTACATGGATGACAAGGAGCTCGAGCTCTACCGCATTTTTCAGGCCATTGATGTTGAACACAACGGCTGCATTCTGCCGGAGGAGCTTTGGGATGCCCTTGTCAAGGCTGGGATTGAAATGGATGATGAGGAACTTGCGCGTTTTGTGGAGCATGTTGATAAGGATAACAATGGAATTATAACTTTTGAAGAATGGAGAGATTTTCTACTACTTTATCCGCATGAAGCAACAATTGAGAATATATATCATCACTGGGAAAGGGTGTTTCTTGTGGATATTGGAGAGCAGGCTGTTATTCCAGAAGGAATTGGTAGGCATGTTCATAGAAGCAGATACTTTATTGCAGGGGCTGTAGCTGGAGCCGCTTCTCGGACTGCCACTGCTCCTCTTGATCGCTTGAAAGTGGTTTTGCAAGTTCAGACATCTCGTGCTTCTGTAGTACCAGCTATCAAGAAGATATTGAAGGATGACGGTATTCTGGGGTTTTTCCGAGGTAACGGAATAAATGTTGTGAAGGTAGCACCTGAAAGTGCCATCAAGTTCTATACTTATGAAATGTTAAAGAAGGTCATTGGAGATAATATGGGGGCAGACGAGTCTGATATTGGTACTGCTGGTAGACTTTTGGCTGGTGGTATGGCAGGTGCAGTGGCACAAACTTCTATCTATCCCTTGGATCTTGTGAAAACTCGGTTACAGACTTGCACTTCAGAAGCTGGAAAGAGTCCTCAGTTGAGGACATTAACTAAAGAAATATGGATTCATGAGGGACCTCGGGCCTTCTATAAAGGTCTGTTTCCGTCACTTCTTGGCATTGTCCCCTACGCTGGCATCGACCTTGCAGCCTATGAGACCTTAAAAGATATGTCAAAGACATATTTTCTTCACGATAATAGCGAACCTGGTCCTCTTATCCAGCTGGGATGTGGGACAATATCTGGAGCTCTAGGAGCAACATGTGTTTATCCATTGCAGGTTATCCGAACAAGATTGCAAGCTCAACGTTCTAATACAGCTACTGCATATAAGGGAATGTCTGATGTATTCTGGAGAACCATTCAACACGAAGGCTATAGAGGTTTCTACAAGGGATTGTTTCCAAATCTTCTCAAAGTTGTTCCAGCTGCAAGCATTACATATATGGTATATGAAGCCATGAAAAAGAAACTAGATCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0006950 GO:0006979 GO:0008150 GO:0008509 GO:0008514 GO:0009987 GO:0010035 GO:0010038 GO:0010941 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0019866 GO:0022857 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0033554 GO:0034220 GO:0034599 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0051592 GO:0051716 GO:0055085 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071277 GO:0071702 GO:0071705 GO:0098656 GO:1901264 GO:1901505 GO:1901679

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_5 EF-hand_7 EF-hand_8 Mito_carr

Protein Analysis

512

Amino Acids

56.85

Weight (kDa)

5.91

Isoelectric Point (pI)

34.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	60 - 121	2e-06	EF-hand domain pair
EF-hand_7	PF13499	127 - 183	5.7e-10	EF-hand domain pair
Mito_carr	PF00153	230 - 312	2e-24	Mitochondrial carrier protein
Mito_carr	PF00153	323 - 410	3.2e-24	Mitochondrial carrier protein
Mito_carr	PF00153	422 - 509	3.7e-25	Mitochondrial carrier protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000767)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07320 AT5G51050 AT5G61810 AT5G61810
fragaria_vesca	FvH4_5g09810 FvH4_5g09810 FvH4_5g09810 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390
malus_domestica	MD00G1075800.v1.1 MD14G1145100.v1.1 MD17G1005800.v1.1
prunus_persica	Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.5G139700_v2.0.a1 Prupe.5G139700_v2.0.a1 Prupe.5G139700_v2.0.a1
pyrus_communis	pycom06g11980 pycom111g00140 pycom17g00150
rosa_chinensis	RchiOBHm_Chr2g0176621 RchiOBHm_Chr7g0188421
rosa_laevigata	RLG00000004696 RLG00000022430
rosa_multiflora	Rmu_sc0000952.1_g000002 Rmu_sc0001417.1_g000014
rosa_roxburghii	Rroxscaffold_2G00076260 Rroxscaffold_3G00266430
rosa_rugosa	Rorug02G0596400 Rorug02G0596500 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596700 Rorug02G0596800 Rorug02G0596900 Rorug06G0490400 Rorug06G0490400
rosa_samantha	Rh2AG674500 Rh2BG685600 Rh2CG648900 Rh2DG699800 Rh7AG095100 Rh7BG096600 Rh7CG097300 Rh7DG096800
rosa_wichuraiana	Rw0G015640 Rw2G055350 Rw7G008200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	536
AarI	CACCTGC	1 cut(s)	989
AasI	GACNNNNNNGTC	1 cut(s)	173
AatII	GACGTC	1 cut(s)	177
Acc36I	ACCTGC	2 cut(s)	989, 1321
AccI	GTMKAC	1 cut(s)	976
AccII	CGCG	3 cut(s)	23, 153, 496
AciI	CCGC	9 cut(s)	21, 23, 75, 114, 272, 355, 388, 575, 720
AclI	AACGTT	1 cut(s)	1360
AclWI	GGATC	3 cut(s)	138, 222, 1041
AcoI	YGGCCR	1 cut(s)	18
AcsI	RAATTY	1 cut(s)	180
AcuI	CTGAAG	3 cut(s)	248, 323, 1050
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	4 cut(s)	287, 359, 798, 967
AfiI	CCNNNNNNNGG	3 cut(s)	460, 726, 1184
AflIII	ACRYGT	1 cut(s)	1313
AgsI	TTSAA	7 cut(s)	196, 413, 473, 545, 757, 820, 1418
AjnI	CCWGG	1 cut(s)	1258
Alw21I	GWGCWC	3 cut(s)	378, 384, 1303
Alw26I	GTCTC	2 cut(s)	149, 1202
AlwI	GGATC	3 cut(s)	138, 222, 1041
AlwNI	CAGNNNCTG	3 cut(s)	707, 1073, 1376
Ama87I	CYCGRG	2 cut(s)	377, 1131
AoxI	GGCC	5 cut(s)	18, 246, 258, 399, 1134
ApeKI	GCWGC	3 cut(s)	423, 1199, 1481
ApoI	RAATTY	1 cut(s)	180
AspLEI	GCGC	2 cut(s)	153, 496
AspS9I	GGNCC	5 cut(s)	116, 259, 1127, 1134, 1262
AsuII	TTCGAA	1 cut(s)	184
AvaI	CYCGRG	2 cut(s)	377, 1131
AvaII	GGWCC	3 cut(s)	116, 1127, 1262
BanII	GRGCYC	3 cut(s)	378, 384, 1303
BauI	CACGAG	1 cut(s)	783
Bbv12I	GWGCWC	3 cut(s)	378, 384, 1303
BbvCI	CCTCAGC	1 cut(s)	210
BbvI	GCAGC	3 cut(s)	410, 1211, 1468
BccI	CCATC	3 cut(s)	121, 320, 899
BceAI	ACGGC	1 cut(s)	436
BciT130I	CCWGG	1 cut(s)	1260
BcoDI	GTCTC	2 cut(s)	149, 1202
BfaI	CTAG	3 cut(s)	143, 1304, 1526
BfmI	CTRYAG	4 cut(s)	708, 792, 1429, 1532
BfuAI	ACCTGC	2 cut(s)	989, 1321
BglII	AGATCT	1 cut(s)	1528
BisI	GCNGC	6 cut(s)	21, 24, 424, 720, 1200, 1482
BlsI	GCNGC	6 cut(s)	22, 25, 425, 721, 1201, 1483
Bme1390I	CCNGG	1 cut(s)	1260
Bme18I	GGWCC	3 cut(s)	116, 1127, 1262
BmeT110I	CYCGRG	2 cut(s)	377, 1131
BmgT120I	GGNCC	5 cut(s)	116, 259, 1127, 1134, 1262
BmiI	GGNNCC	3 cut(s)	118, 718, 1128
BmrFI	CCNGG	1 cut(s)	1260
BmrI	ACTGGG	1 cut(s)	619
BmsI	GCATC	5 cut(s)	43, 196, 304, 439, 1197
BmuI	ACTGGG	1 cut(s)	619
BpmI	CTGGAG	3 cut(s)	735, 1317, 1426
Bpu10I	CCTNAGC	1 cut(s)	210
Bpu14I	TTCGAA	1 cut(s)	184
BpuEI	CTTGAG	1 cut(s)	365
BsaHI	GRCGYC	1 cut(s)	174
BsaI	GGTCTC	2 cut(s)	149, 1202
BsaJI	CCNNGG	5 cut(s)	21, 159, 847, 1029, 1130
BsaWI	WCCGGW	1 cut(s)	100
Bsc4I	CCNNNNNNNGG	3 cut(s)	460, 726, 1184
Bse118I	RCCGGY	1 cut(s)	16
Bse1I	ACTGG	1 cut(s)	614
Bse3DI	GCAATG	1 cut(s)	1325
BseBI	CCWGG	1 cut(s)	1260
BseDI	CCNNGG	5 cut(s)	21, 159, 847, 1029, 1130
BseGI	GGATG	8 cut(s)	58, 95, 331, 370, 454, 484, 829, 1286
BseLI	CCNNNNNNNGG	3 cut(s)	460, 726, 1184
BseMI	GCAATG	1 cut(s)	1325
BseMII	CTCAG	3 cut(s)	201, 251, 1100
BseNI	ACTGG	1 cut(s)	614
BseRI	GAGGAG	2 cut(s)	452, 732
BseX3I	CGGCCG	1 cut(s)	18
BseXI	GCAGC	3 cut(s)	410, 1211, 1468
BseYI	CCCAGC	2 cut(s)	464, 1276
BsgI	GTGCAG	1 cut(s)	1023
Bsh1236I	CGCG	3 cut(s)	23, 153, 496
Bsh1285I	CGRYCG	1 cut(s)	21
BshFI	GGCC	5 cut(s)	20, 248, 260, 401, 1136
BsiEI	CGRYCG	1 cut(s)	21
BsiHKAI	GWGCWC	3 cut(s)	378, 384, 1303
BsiHKCI	CYCGRG	2 cut(s)	377, 1131
BsiSI	CCGG	5 cut(s)	17, 101, 249, 352, 434
BslFI	GGGAC	4 cut(s)	185, 1140, 1160, 1300
BslI	CCNNNNNNNGG	3 cut(s)	460, 726, 1184
BsmAI	GTCTC	2 cut(s)	149, 1202
BsmFI	GGGAC	4 cut(s)	185, 1140, 1160, 1300
BsmI	GAATGC	1 cut(s)	426
BsnI	GGCC	5 cut(s)	20, 248, 260, 401, 1136
Bso31I	GGTCTC	2 cut(s)	149, 1202
BsoBI	CYCGRG	2 cut(s)	377, 1131
Bsp119I	TTCGAA	1 cut(s)	184
Bsp1286I	GDGCHC	3 cut(s)	378, 384, 1303
Bsp143I	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
BspACI	CCGC	9 cut(s)	21, 23, 75, 114, 272, 355, 388, 575, 720
BspANI	GGCC	5 cut(s)	20, 248, 260, 401, 1136
BspCNI	CTCAG	3 cut(s)	202, 250, 1099
BspFNI	CGCG	3 cut(s)	23, 153, 496
BspHI	TCATGA	1 cut(s)	1120
BspLI	GGNNCC	3 cut(s)	118, 718, 1128
BspMI	ACCTGC	2 cut(s)	989, 1321
BspPI	GGATC	3 cut(s)	138, 222, 1041
BspT104I	TTCGAA	1 cut(s)	184
BspTNI	GGTCTC	2 cut(s)	149, 1202
BsrDI	GCAATG	1 cut(s)	1325
BsrFI	RCCGGY	1 cut(s)	16
BsrI	ACTGG	1 cut(s)	614
BssAI	RCCGGY	1 cut(s)	16
BssECI	CCNNGG	5 cut(s)	21, 159, 847, 1029, 1130
BssMI	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
BssNI	GRCGYC	1 cut(s)	174
BssSI	CACGAG	1 cut(s)	783
BssT1I	CCWWGG	2 cut(s)	159, 1029
Bst2BI	CACGAG	1 cut(s)	783
Bst2UI	CCWGG	1 cut(s)	1260
Bst4CI	ACNGT	1 cut(s)	830
BstACI	GRCGYC	1 cut(s)	174
BstBI	TTCGAA	1 cut(s)	184
BstC8I	GCNNGC	5 cut(s)	18, 645, 1186, 1353, 1486
BstDEI	CTNAG	3 cut(s)	210, 237, 1086
BstDSI	CCRYGG	1 cut(s)	21
BstENI	CCTNNNNNAGG	1 cut(s)	458
BstF5I	GGATG	8 cut(s)	58, 95, 331, 370, 454, 484, 829, 1286
BstFNI	CGCG	3 cut(s)	23, 153, 496
BstHHI	GCGC	2 cut(s)	153, 496
BstKTI	GATC	7 cut(s)	133, 217, 243, 298, 751, 1036, 1531
BstMAI	GTCTC	2 cut(s)	149, 1202
BstMBI	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
BstMCI	CGRYCG	1 cut(s)	21
BstMWI	GCNNNNNNNGC	4 cut(s)	17, 439, 719, 1307
BstNI	CCWGG	1 cut(s)	1260
BstNSI	RCATGY	4 cut(s)	35, 512, 677, 1317
BstSCI	CCNGG	1 cut(s)	1258
BstSFI	CTRYAG	4 cut(s)	708, 792, 1429, 1532
BstUI	CGCG	3 cut(s)	23, 153, 496
BstV1I	GCAGC	3 cut(s)	410, 1211, 1468
BstX2I	RGATCY	3 cut(s)	214, 1033, 1528
BstXI	CCANNNNNNTGG	1 cut(s)	127
BstYI	RGATCY	3 cut(s)	214, 1033, 1528
BstZI	CGGCCG	1 cut(s)	18
BsuRI	GGCC	5 cut(s)	20, 248, 260, 401, 1136
BtgI	CCRYGG	1 cut(s)	21
BtsCI	GGATG	8 cut(s)	58, 95, 331, 370, 454, 484, 829, 1286
BtsI	GCAGTG	3 cut(s)	66, 735, 1011
BtsIMutI	CAGTG	4 cut(s)	66, 607, 735, 1011
BveI	ACCTGC	2 cut(s)	989, 1321
Cac8I	GCNNGC	5 cut(s)	18, 645, 1186, 1353, 1486
CaiI	CAGNNNCTG	3 cut(s)	707, 1073, 1376
CciI	TCATGA	1 cut(s)	1120
CfoI	GCGC	2 cut(s)	153, 496
Cfr10I	RCCGGY	1 cut(s)	16
Cfr13I	GGNCC	5 cut(s)	116, 259, 1127, 1134, 1262
Cfr42I	CCGCGG	1 cut(s)	24
CsiI	ACCWGGT	1 cut(s)	1258
Csp6I	GTAC	4 cut(s)	286, 358, 797, 966
CviAII	CATG	8 cut(s)	32, 361, 509, 578, 674, 1121, 1314, 1513
CviQI	GTAC	4 cut(s)	286, 358, 797, 966
DdeI	CTNAG	3 cut(s)	210, 237, 1086
DpnI	GATC	7 cut(s)	132, 216, 242, 297, 750, 1035, 1530
DpnII	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
DrdI	GACNNNNNNGTC	1 cut(s)	173
DseDI	GACNNNNNNGTC	1 cut(s)	173
EaeI	YGGCCR	1 cut(s)	18
EagI	CGGCCG	1 cut(s)	18
EciI	GGCGGA	1 cut(s)	261
Ecl136II	GAGCTC	3 cut(s)	376, 382, 1301
EclXI	CGGCCG	1 cut(s)	18
Eco130I	CCWWGG	2 cut(s)	159, 1029
Eco24I	GRGCYC	3 cut(s)	378, 384, 1303
Eco31I	GGTCTC	2 cut(s)	149, 1202
Eco47I	GGWCC	3 cut(s)	116, 1127, 1262
Eco52I	CGGCCG	1 cut(s)	18
Eco53kI	GAGCTC	3 cut(s)	376, 382, 1301
Eco57I	CTGAAG	3 cut(s)	248, 323, 1050
Eco88I	CYCGRG	2 cut(s)	377, 1131
EcoICRI	GAGCTC	3 cut(s)	376, 382, 1301
EcoNI	CCTNNNNNAGG	1 cut(s)	458
EcoO109I	RGGNCCY	1 cut(s)	1127
EcoRI	GAATTC	1 cut(s)	180
EcoRII	CCWGG	1 cut(s)	1258
EcoT14I	CCWWGG	2 cut(s)	159, 1029
EcoT38I	GRGCYC	3 cut(s)	378, 384, 1303
ErhI	CCWWGG	2 cut(s)	159, 1029
FaeI	CATG	8 cut(s)	35, 364, 512, 581, 677, 1124, 1317, 1516
FaqI	GGGAC	4 cut(s)	185, 1140, 1160, 1300
FatI	CATG	8 cut(s)	31, 360, 508, 577, 673, 1120, 1313, 1512
FblI	GTMKAC	1 cut(s)	976
Fnu4HI	GCNGC	6 cut(s)	21, 24, 424, 720, 1200, 1482
FokI	GGATG	8 cut(s)	65, 102, 338, 377, 461, 491, 836, 1293
FriOI	GRGCYC	3 cut(s)	378, 384, 1303
Fsp4HI	GCNGC	6 cut(s)	21, 24, 424, 720, 1200, 1482
FspBI	CTAG	3 cut(s)	143, 1304, 1526
GlaI	GCGC	2 cut(s)	152, 495
GluI	GCNGC	6 cut(s)	21, 24, 424, 720, 1200, 1482
GsaI	CCCAGC	2 cut(s)	468, 1280
GsuI	CTGGAG	3 cut(s)	735, 1317, 1426
HaeIII	GGCC	5 cut(s)	20, 248, 260, 401, 1136
HapII	CCGG	5 cut(s)	17, 101, 249, 352, 434
HhaI	GCGC	2 cut(s)	153, 496
Hin1I	GRCGYC	1 cut(s)	174
Hin1II	CATG	8 cut(s)	35, 364, 512, 581, 677, 1124, 1317, 1516
Hin6I	GCGC	2 cut(s)	151, 494
HinP1I	GCGC	2 cut(s)	151, 494
HinfI	GANTC	4 cut(s)	268, 953, 1081, 1117
HpaII	CCGG	5 cut(s)	17, 101, 249, 352, 434
Hpy166II	GTNNAC	2 cut(s)	66, 977
Hpy188III	TCNNGA	9 cut(s)	106, 344, 656, 746, 808, 1121, 1244, 1296, 1405
Hpy8I	GTNNAC	2 cut(s)	66, 977
HpyCH4III	ACNGT	1 cut(s)	830
HpyCH4IV	ACGT	4 cut(s)	48, 135, 174, 1360
HpyF10VI	GCNNNNNNNGC	4 cut(s)	17, 439, 719, 1307
HpyF3I	CTNAG	3 cut(s)	210, 237, 1086
HpySE526I	ACGT	4 cut(s)	48, 135, 174, 1360
Hsp92I	GRCGYC	1 cut(s)	174
Hsp92II	CATG	8 cut(s)	35, 364, 512, 581, 677, 1124, 1317, 1516
HspAI	GCGC	2 cut(s)	151, 494
KroI	GCCGGC	1 cut(s)	16
KroNI	GCCGGC	1 cut(s)	18
KspI	CCGCGG	1 cut(s)	24
Kzo9I	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
LmnI	GCTCC	7 cut(s)	373, 439, 505, 716, 745, 1298, 1307
Lsp1109I	GCAGC	3 cut(s)	410, 1211, 1468
LweI	GCATC	5 cut(s)	43, 196, 304, 439, 1197
MabI	ACCWGGT	1 cut(s)	1258
MaeI	CTAG	3 cut(s)	143, 1304, 1526
MaeII	ACGT	4 cut(s)	48, 135, 174, 1360
MaeIII	GTNAC	3 cut(s)	851, 1050, 1158
MalI	GATC	7 cut(s)	132, 216, 242, 297, 750, 1035, 1530
MboI	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
MboII	GAAGA	7 cut(s)	131, 193, 290, 557, 823, 1232, 1454
MfeI	CAATTG	1 cut(s)	588
MflI	RGATCY	3 cut(s)	214, 1033, 1528
MhlI	GDGCHC	3 cut(s)	378, 384, 1303
MluCI	AATT	5 cut(s)	180, 196, 531, 588, 663
MlyI	GAGTC	2 cut(s)	962, 1090
MroNI	GCCGGC	1 cut(s)	16
MseI	TTAA	3 cut(s)	917, 1100, 1214
MslI	CAYNNNNRTG	1 cut(s)	125
MspA1I	CMGCKG	3 cut(s)	23, 1276, 1481
MspI	CCGG	5 cut(s)	17, 101, 249, 352, 434
MspR9I	CCNGG	1 cut(s)	1260
MunI	CAATTG	1 cut(s)	588
Mva1269I	GAATGC	1 cut(s)	426
MvaI	CCWGG	1 cut(s)	1260
MvnI	CGCG	3 cut(s)	23, 153, 496
MwoI	GCNNNNNNNGC	4 cut(s)	17, 439, 719, 1307
NaeI	GCCGGC	1 cut(s)	18
NdeII	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
NgoMIV	GCCGGC	1 cut(s)	16
NlaIII	CATG	8 cut(s)	35, 364, 512, 581, 677, 1124, 1317, 1516
NlaIV	GGNNCC	3 cut(s)	118, 718, 1128
NmuCI	GTSAC	1 cut(s)	1158
NspI	RCATGY	4 cut(s)	35, 512, 677, 1317
NspV	TTCGAA	1 cut(s)	184
PaeR7I	CTCGAG	1 cut(s)	377
PagI	TCATGA	1 cut(s)	1120
PaqCI	CACCTGC	1 cut(s)	989
PciI	ACATGT	1 cut(s)	1313
PcsI	WCGNNNNNNNCGW	2 cut(s)	1188, 1251
PctI	GAATGC	1 cut(s)	426
PdiI	GCCGGC	1 cut(s)	18
PfeI	GAWTC	2 cut(s)	268, 1117
PkrI	GCNGC	6 cut(s)	22, 25, 425, 721, 1201, 1483
PleI	GAGTC	2 cut(s)	961, 1089
PpsI	GAGTC	2 cut(s)	961, 1089
PpuMI	RGGWCCY	1 cut(s)	1127
PscI	ACATGT	1 cut(s)	1313
PsiI	TTATAA	1 cut(s)	536
Psp124BI	GAGCTC	3 cut(s)	378, 384, 1303
Psp1406I	AACGTT	1 cut(s)	1360
Psp5II	RGGWCCY	1 cut(s)	1127
Psp6I	CCWGG	1 cut(s)	1258
PspFI	CCCAGC	2 cut(s)	464, 1276
PspGI	CCWGG	1 cut(s)	1258
PspN4I	GGNNCC	3 cut(s)	118, 718, 1128
PspPI	GGNCC	5 cut(s)	116, 259, 1127, 1134, 1262
PspPPI	RGGWCCY	1 cut(s)	1127
PspXI	VCTCGAGB	1 cut(s)	377
PstNI	CAGNNNCTG	3 cut(s)	707, 1073, 1376
PsuI	RGATCY	3 cut(s)	214, 1033, 1528
PvuII	CAGCTG	2 cut(s)	1276, 1481
RsaI	GTAC	4 cut(s)	287, 359, 798, 967
RsaNI	GTAC	4 cut(s)	286, 358, 797, 966
RseI	CAYNNNNRTG	1 cut(s)	125
SacI	GAGCTC	3 cut(s)	378, 384, 1303
SacII	CCGCGG	1 cut(s)	24
SaqAI	TTAA	3 cut(s)	917, 1100, 1214
SatI	GCNGC	6 cut(s)	21, 24, 424, 720, 1200, 1482
Sau3AI	GATC	7 cut(s)	130, 214, 240, 295, 748, 1033, 1528
Sau96I	GGNCC	5 cut(s)	116, 259, 1127, 1134, 1262
SchI	GAGTC	2 cut(s)	962, 1090
ScrFI	CCNGG	1 cut(s)	1260
SduI	GDGCHC	3 cut(s)	378, 384, 1303
SexAI	ACCWGGT	1 cut(s)	1258
SfaNI	GCATC	5 cut(s)	43, 196, 304, 439, 1197
SfcI	CTRYAG	4 cut(s)	708, 792, 1429, 1532
Sfr274I	CTCGAG	1 cut(s)	377
Sfr303I	CCGCGG	1 cut(s)	24
SfuI	TTCGAA	1 cut(s)	184
SgrBI	CCGCGG	1 cut(s)	24
SinI	GGWCC	3 cut(s)	116, 1127, 1262
SlaI	CTCGAG	1 cut(s)	377
SmiMI	CAYNNNNRTG	1 cut(s)	125
SmlI	CTYRAG	2 cut(s)	344, 377
SmoI	CTYRAG	2 cut(s)	344, 377
Sse9I	AATT	5 cut(s)	180, 196, 531, 588, 663
SsiI	CCGC	9 cut(s)	21, 23, 75, 114, 272, 355, 388, 575, 720
SspMI	CTAG	3 cut(s)	143, 1304, 1526
SstI	GAGCTC	3 cut(s)	378, 384, 1303
StyD4I	CCNGG	1 cut(s)	1258
StyI	CCWWGG	2 cut(s)	159, 1029
TaaI	ACNGT	1 cut(s)	830
TaiI	ACGT	4 cut(s)	51, 138, 177, 1363
TaqI	TCGA	7 cut(s)	125, 184, 204, 243, 336, 378, 1191
TasI	AATT	5 cut(s)	180, 196, 531, 588, 663
TauI	GCSGC	3 cut(s)	23, 26, 722
TfiI	GAWTC	2 cut(s)	268, 1117
Tru1I	TTAA	3 cut(s)	917, 1100, 1214
Tru9I	TTAA	3 cut(s)	917, 1100, 1214
TscAI	CASTG	4 cut(s)	73, 614, 742, 1011
TseFI	GTSAC	1 cut(s)	1158
TseI	GCWGC	3 cut(s)	423, 1199, 1481
Tsp45I	GTSAC	1 cut(s)	1158
TspDTI	ATGAA	6 cut(s)	594, 668, 924, 1109, 1521, 1529
TspGWI	ACGGA	2 cut(s)	870, 1146
TspRI	CASTG	4 cut(s)	73, 614, 742, 1011
VpaK11BI	GGWCC	3 cut(s)	116, 1127, 1262
XagI	CCTNNNNNAGG	1 cut(s)	458
XapI	RAATTY	1 cut(s)	180
XceI	RCATGY	4 cut(s)	35, 512, 677, 1317
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1280
XhoI	CTCGAG	1 cut(s)	377
XmiI	GTMKAC	1 cut(s)	976
XspI	CTAG	3 cut(s)	143, 1304, 1526
ZraI	GACGTC	1 cut(s)	175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.