Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000767

Physical Location & Seq

Genomic Coordinates Reverse (-)

23809045 .. 23811996

Gene Structure 2952 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1145100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGTCAGGGGCAAAGCCTGCCGCGGCAGAGCATGTCAGCTTCTCCAAAGTAGATGTGAACAAGTCCACCGCCTCCGCCACCGCCAGAGCCGACGGATGTTGCAATCCGGTCAAGAAAACTGGACCCATTTCGATGGATCACGTTCTTCTAGCGTTACGGGAGACCAAGGAGGACAGGGAAGTCCGAATTCGAAGCCTTTTCAATTTCTTCGATGCTGAAAATCTTGGGTATTTGGATTATTCTCAGATCGAGGCCGGACTGTCGGCCCTTCAGATTCCTCCGGAGTACAAGTACGCCAAGGATCTTCTGAAGGTCTGTGATGCCAACAGAGATGGAAGGGTCGATTATCCCGAGTTCCGGCGGTACATGGATGACAAGGAGCTCGAGCTCTACCGCATTTTTCAGGCCATTGATGTTGAGCACAATGGCTGCATTCTGCCTGAGGAGCTATGGGATGCCCTTGTCAAGGCTGGGATTGAAATGGATGATGAGGAACTTGCCCGGTTTGTAGAGCATGTTGATAAGGATAACAATGGTATTATAACGTTTGAAGAATGGAGGGATTTTCTTCTACTGTATCCACATGAAGCAACGATTGAGAACATATATCATCATTGGGAAAGGGTGTGCCTTGTGGATATTGGTGAGCAGGCTGTTATTCCAGAAGGAATTGGTAAGCATGTTCATAGGAGCAGATACTTTATTGCAGGTGCTATAGCTGGAGCCGCTTCTCGTACTGCCACTGCTCCTCTTGATCGCTTGAAAGTGGTTTTGCAAGTTCAGACATCACGTGCTTCTGTGGTGCCAGCTATAAAGAAGATATTGAAGGAAGACGGTATCCTGGGGTTTTTCAGAGGTAATGGAATAAATGTTGTGAAGGTAGCACCTGAAAGTGCCATCAAGTTCTATACTTATGAAATGTTAAAGAAGGTCATTGGAGATAATATGGGGGCCAACGAGGGAGATATTGGTACTGCTGGTAGACTTTTGGCCGGTGGTATGGCAGGTGCAGTGGCACAAACTTCTATCTATCCGTTGGATCTTGTGAAAACTCGGTTACAAACTTGTACTTCAGAAGCTGGAAAGAGTCCTCGGTTGGGGACATTAACTAGGGAAATATGGATTCATGAGGGACCTCGGGCCTTCTATAAAGGTCTGCTTCCGTCTCTTCTTGGGATTGTCCCCTATGCTGGTATCGACCTCACTGCCTATGAGACCTTAAAAGATATGTCAAAGACATATTTTCTTCACGATAATAGCGAACCTGGTCCTCTCATTCAGCTGGGATGTGGGACAATATCGGGAGCTCTAGGAGCAACATGTGTTTATCCATTGCAGGTTATCCGAACCAGATTGCAAGCTCAACGTTCTAATACAGCTACTGCATATAAAGGAATGTCTGATGTATTCTGGAGAACCCTTCAGCACGAAGGCTATAGAGGTTTCTACAAGGGATTGTTTCCAAATCTTCTCAAAGTTGTTCCAGCTGCAAGCATTACATATATGGTATACGAAGCCATGAAAAAGAAACTAGATCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0006950 GO:0006979 GO:0008150 GO:0008509 GO:0008514 GO:0009987 GO:0010035 GO:0010038 GO:0010941 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0019866 GO:0022857 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0033554 GO:0034220 GO:0034599 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0051592 GO:0051716 GO:0055085 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071277 GO:0071702 GO:0071705 GO:0098656 GO:1901264 GO:1901505 GO:1901679

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_5 EF-hand_7 EF-hand_8 Mito_carr

Protein Analysis

514

Amino Acids

57.01

Weight (kDa)

6.34

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	61 - 123	3.6e-07	EF-hand domain pair
EF-hand_7	PF13499	129 - 185	5.7e-10	EF-hand domain pair
Mito_carr	PF00153	231 - 314	4.6e-25	Mitochondrial carrier protein
Mito_carr	PF00153	325 - 412	4.3e-24	Mitochondrial carrier protein
Mito_carr	PF00153	424 - 511	4.9e-25	Mitochondrial carrier protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000767)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07320 AT5G51050 AT5G61810 AT5G61810
fragaria_vesca	FvH4_5g09810 FvH4_5g09810 FvH4_5g09810 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390 FvH4_6g54390
malus_domestica	MD00G1075800.v1.1 MD14G1145100.v1.1 MD17G1005800.v1.1
prunus_persica	Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.3G314500_v2.0.a1 Prupe.5G139700_v2.0.a1 Prupe.5G139700_v2.0.a1 Prupe.5G139700_v2.0.a1
pyrus_communis	pycom06g11980 pycom111g00140 pycom17g00150
rosa_chinensis	RchiOBHm_Chr2g0176621 RchiOBHm_Chr7g0188421
rosa_laevigata	RLG00000004696 RLG00000022430
rosa_multiflora	Rmu_sc0000952.1_g000002 Rmu_sc0001417.1_g000014
rosa_roxburghii	Rroxscaffold_2G00076260 Rroxscaffold_3G00266430
rosa_rugosa	Rorug02G0596400 Rorug02G0596500 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596600 Rorug02G0596700 Rorug02G0596800 Rorug02G0596900 Rorug06G0490400 Rorug06G0490400
rosa_samantha	Rh2AG674500 Rh2BG685600 Rh2CG648900 Rh2DG699800 Rh7AG095100 Rh7BG096600 Rh7CG097300 Rh7DG096800
rosa_wichuraiana	Rw0G015640 Rw2G055350 Rw7G008200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	542
AarI	CACCTGC	2 cut(s)	698, 995
AasI	GACNNNNNNGTC	1 cut(s)	179
Acc36I	ACCTGC	3 cut(s)	698, 995, 1327
AccB1I	GGYRCC	1 cut(s)	802
AccI	GTMKAC	2 cut(s)	982, 1509
AccII	CGCG	1 cut(s)	23
AccIII	TCCGGA	1 cut(s)	280
AciI	CCGC	8 cut(s)	21, 23, 69, 75, 81, 361, 394, 726
AclI	AACGTT	2 cut(s)	545, 1366
AclWI	GGATC	3 cut(s)	144, 309, 1047
AcoI	YGGCCR	1 cut(s)	990
AcsI	RAATTY	1 cut(s)	186
AcuI	CTGAAG	4 cut(s)	254, 329, 1056, 1406
AcvI	CACGTG	1 cut(s)	791
AfaI	GTAC	6 cut(s)	287, 293, 365, 736, 973, 1069
AfiI	CCNNNNNNNGG	4 cut(s)	357, 466, 1097, 1190
AflIII	ACRYGT	1 cut(s)	1319
AgsI	TTSAA	5 cut(s)	202, 479, 551, 763, 826
AjnI	CCWGG	2 cut(s)	840, 1264
Alw21I	GWGCWC	4 cut(s)	384, 390, 423, 1309
Alw26I	GTCTC	3 cut(s)	155, 1170, 1208
AlwI	GGATC	3 cut(s)	144, 309, 1047
AlwNI	CAGNNNCTG	2 cut(s)	1079, 1382
Ama87I	CYCGRG	3 cut(s)	350, 383, 1137
Aor13HI	TCCGGA	1 cut(s)	280
AoxI	GGCC	6 cut(s)	252, 264, 405, 951, 990, 1140
ApeKI	GCWGC	2 cut(s)	429, 1487
ApoI	RAATTY	1 cut(s)	186
AspS9I	GGNCC	6 cut(s)	122, 265, 951, 1133, 1140, 1268
AsuC2I	CCSGG	1 cut(s)	502
AsuHPI	GGTGA	1 cut(s)	656
AsuII	TTCGAA	1 cut(s)	190
AvaI	CYCGRG	3 cut(s)	350, 383, 1137
AvaII	GGWCC	3 cut(s)	122, 1133, 1268
AxyI	CCTNAGG	1 cut(s)	441
BanI	GGYRCC	1 cut(s)	802
BanII	GRGCYC	3 cut(s)	384, 390, 1309
BbrPI	CACGTG	1 cut(s)	791
BbsI	GAAGAC	1 cut(s)	837
Bbv12I	GWGCWC	4 cut(s)	384, 390, 423, 1309
BbvI	GCAGC	2 cut(s)	416, 1474
BccI	CCATC	3 cut(s)	127, 326, 905
BcgI	CGANNNNNNTGC	2 cut(s)	1187, 1221
BciT130I	CCWGG	2 cut(s)	842, 1266
BciVI	GTATCC	2 cut(s)	588, 848
BcnI	CCSGG	1 cut(s)	502
BcoDI	GTCTC	3 cut(s)	155, 1170, 1208
BfaI	CTAG	4 cut(s)	149, 1110, 1310, 1532
BfmI	CTRYAG	3 cut(s)	714, 1435, 1538
BfuAI	ACCTGC	3 cut(s)	698, 995, 1327
BfuI	GTATCC	2 cut(s)	588, 848
BglII	AGATCT	1 cut(s)	1534
BisI	GCNGC	5 cut(s)	21, 24, 430, 726, 1488
BlsI	GCNGC	5 cut(s)	22, 25, 431, 727, 1489
Bme1390I	CCNGG	3 cut(s)	502, 842, 1266
Bme18I	GGWCC	3 cut(s)	122, 1133, 1268
BmeT110I	CYCGRG	3 cut(s)	350, 383, 1137
BmgT120I	GGNCC	6 cut(s)	122, 265, 951, 1133, 1140, 1268
BmiI	GGNNCC	5 cut(s)	124, 724, 804, 952, 1134
BmrFI	CCNGG	3 cut(s)	502, 842, 1266
BmsI	GCATC	3 cut(s)	202, 310, 445
BpiI	GAAGAC	1 cut(s)	837
BpmI	CTGGAG	2 cut(s)	741, 1432
Bpu14I	TTCGAA	1 cut(s)	190
BpuMI	CCSGG	1 cut(s)	502
BsaAI	YACGTR	1 cut(s)	791
BsaI	GGTCTC	2 cut(s)	155, 1208
BsaJI	CCNNGG	6 cut(s)	21, 165, 297, 841, 1091, 1136
BsaWI	WCCGGW	2 cut(s)	106, 280
BsaXI	ACNNNNNCTCC	2 cut(s)	275, 305
Bsc4I	CCNNNNNNNGG	4 cut(s)	357, 466, 1097, 1190
Bse118I	RCCGGY	1 cut(s)	992
Bse1I	ACTGG	1 cut(s)	124
Bse21I	CCTNAGG	1 cut(s)	441
Bse3DI	GCAATG	1 cut(s)	1331
BseAI	TCCGGA	1 cut(s)	280
BseBI	CCWGG	2 cut(s)	842, 1266
BseDI	CCNNGG	6 cut(s)	21, 165, 297, 841, 1091, 1136
BseGI	GGATG	5 cut(s)	101, 376, 460, 490, 1292
BseLI	CCNNNNNNNGG	4 cut(s)	357, 466, 1097, 1190
BseMI	GCAATG	1 cut(s)	1331
BseMII	CTCAG	2 cut(s)	257, 432
BseNI	ACTGG	1 cut(s)	124
BseRI	GAGGAG	2 cut(s)	458, 738
BseXI	GCAGC	2 cut(s)	416, 1474
BseYI	CCCAGC	2 cut(s)	470, 1282
BsgI	GTGCAG	1 cut(s)	1029
Bsh1236I	CGCG	1 cut(s)	23
BshFI	GGCC	6 cut(s)	254, 266, 407, 953, 992, 1142
BshNI	GGYRCC	1 cut(s)	802
BsiHKAI	GWGCWC	4 cut(s)	384, 390, 423, 1309
BsiHKCI	CYCGRG	3 cut(s)	350, 383, 1137
BsiSI	CCGG	6 cut(s)	107, 255, 281, 358, 502, 993
BslFI	GGGAC	4 cut(s)	1114, 1146, 1166, 1306
BslI	CCNNNNNNNGG	4 cut(s)	357, 466, 1097, 1190
BsmAI	GTCTC	3 cut(s)	155, 1170, 1208
BsmBI	CGTCTC	1 cut(s)	1170
BsmFI	GGGAC	4 cut(s)	1114, 1146, 1166, 1306
BsmI	GAATGC	1 cut(s)	432
BsnI	GGCC	6 cut(s)	254, 266, 407, 953, 992, 1142
Bso31I	GGTCTC	2 cut(s)	155, 1208
BsoBI	CYCGRG	3 cut(s)	350, 383, 1137
Bsp119I	TTCGAA	1 cut(s)	190
Bsp1286I	GDGCHC	4 cut(s)	384, 390, 423, 1309
Bsp13I	TCCGGA	1 cut(s)	280
Bsp143I	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
BspACI	CCGC	8 cut(s)	21, 23, 69, 75, 81, 361, 394, 726
BspANI	GGCC	6 cut(s)	254, 266, 407, 953, 992, 1142
BspCNI	CTCAG	2 cut(s)	256, 433
BspEI	TCCGGA	1 cut(s)	280
BspFNI	CGCG	1 cut(s)	23
BspHI	TCATGA	1 cut(s)	1126
BspLI	GGNNCC	5 cut(s)	124, 724, 804, 952, 1134
BspMI	ACCTGC	3 cut(s)	698, 995, 1327
BspPI	GGATC	3 cut(s)	144, 309, 1047
BspT104I	TTCGAA	1 cut(s)	190
BspT107I	GGYRCC	1 cut(s)	802
BspTNI	GGTCTC	2 cut(s)	155, 1208
BsrDI	GCAATG	1 cut(s)	1331
BsrFI	RCCGGY	1 cut(s)	992
BsrI	ACTGG	1 cut(s)	124
BssAI	RCCGGY	1 cut(s)	992
BssECI	CCNNGG	6 cut(s)	21, 165, 297, 841, 1091, 1136
BssMI	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
BssNAI	GTATAC	1 cut(s)	1510
BssT1I	CCWWGG	2 cut(s)	165, 297
Bst1107I	GTATAC	1 cut(s)	1510
Bst2UI	CCWGG	2 cut(s)	842, 1266
Bst4CI	ACNGT	3 cut(s)	261, 576, 836
Bst6I	CTCTTC	1 cut(s)	1173
BstAPI	GCANNNNNTGC	1 cut(s)	17
BstBAI	YACGTR	1 cut(s)	791
BstBI	TTCGAA	1 cut(s)	190
BstC8I	GCNNGC	5 cut(s)	18, 651, 807, 1359, 1492
BstDEI	CTNAG	2 cut(s)	243, 441
BstDSI	CCRYGG	1 cut(s)	21
BstENI	CCTNNNNNAGG	1 cut(s)	464
BstF5I	GGATG	5 cut(s)	101, 376, 460, 490, 1292
BstFNI	CGCG	1 cut(s)	23
BstKTI	GATC	6 cut(s)	139, 249, 304, 757, 1042, 1537
BstMAI	GTCTC	3 cut(s)	155, 1170, 1208
BstMBI	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
BstMWI	GCNNNNNNNGC	4 cut(s)	17, 445, 725, 1313
BstNI	CCWGG	2 cut(s)	842, 1266
BstNSI	RCATGY	4 cut(s)	35, 518, 683, 1323
BstSCI	CCNGG	3 cut(s)	500, 840, 1264
BstSFI	CTRYAG	3 cut(s)	714, 1435, 1538
BstUI	CGCG	1 cut(s)	23
BstV1I	GCAGC	2 cut(s)	416, 1474
BstV2I	GAAGAC	1 cut(s)	837
BstX2I	RGATCY	3 cut(s)	301, 1039, 1534
BstXI	CCANNNNNNTGG	1 cut(s)	133
BstYI	RGATCY	3 cut(s)	301, 1039, 1534
BstZ17I	GTATAC	1 cut(s)	1510
Bsu36I	CCTNAGG	1 cut(s)	441
BsuI	GTATCC	2 cut(s)	588, 848
BsuRI	GGCC	6 cut(s)	254, 266, 407, 953, 992, 1142
BtgI	CCRYGG	1 cut(s)	21
BtsCI	GGATG	5 cut(s)	101, 376, 460, 490, 1292
BtsI	GCAGTG	3 cut(s)	741, 1017, 1203
BtsIMutI	CAGTG	3 cut(s)	741, 1017, 1203
BveI	ACCTGC	3 cut(s)	698, 995, 1327
Cac8I	GCNNGC	5 cut(s)	18, 651, 807, 1359, 1492
CaiI	CAGNNNCTG	2 cut(s)	1079, 1382
CciI	TCATGA	1 cut(s)	1126
Cfr10I	RCCGGY	1 cut(s)	992
Cfr13I	GGNCC	6 cut(s)	122, 265, 951, 1133, 1140, 1268
Cfr42I	CCGCGG	1 cut(s)	24
CsiI	ACCWGGT	1 cut(s)	1264
Csp6I	GTAC	6 cut(s)	286, 292, 364, 735, 972, 1068
CviAII	CATG	8 cut(s)	32, 367, 515, 584, 680, 1127, 1320, 1519
CviQI	GTAC	6 cut(s)	286, 292, 364, 735, 972, 1068
DdeI	CTNAG	2 cut(s)	243, 441
DpnI	GATC	6 cut(s)	138, 248, 303, 756, 1041, 1536
DpnII	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
DrdI	GACNNNNNNGTC	1 cut(s)	179
DseDI	GACNNNNNNGTC	1 cut(s)	179
EaeI	YGGCCR	1 cut(s)	990
Eam1104I	CTCTTC	1 cut(s)	1173
EarI	CTCTTC	1 cut(s)	1173
EciI	GGCGGA	1 cut(s)	64
Ecl136II	GAGCTC	3 cut(s)	382, 388, 1307
Eco130I	CCWWGG	2 cut(s)	165, 297
Eco24I	GRGCYC	3 cut(s)	384, 390, 1309
Eco31I	GGTCTC	2 cut(s)	155, 1208
Eco47I	GGWCC	3 cut(s)	122, 1133, 1268
Eco53kI	GAGCTC	3 cut(s)	382, 388, 1307
Eco57I	CTGAAG	4 cut(s)	254, 329, 1056, 1406
Eco72I	CACGTG	1 cut(s)	791
Eco81I	CCTNAGG	1 cut(s)	441
Eco88I	CYCGRG	3 cut(s)	350, 383, 1137
EcoICRI	GAGCTC	3 cut(s)	382, 388, 1307
EcoNI	CCTNNNNNAGG	1 cut(s)	464
EcoO109I	RGGNCCY	1 cut(s)	1133
EcoRI	GAATTC	1 cut(s)	186
EcoRII	CCWGG	2 cut(s)	840, 1264
EcoT14I	CCWWGG	2 cut(s)	165, 297
EcoT38I	GRGCYC	3 cut(s)	384, 390, 1309
ErhI	CCWWGG	2 cut(s)	165, 297
Esp3I	CGTCTC	1 cut(s)	1170
FaeI	CATG	8 cut(s)	35, 370, 518, 587, 683, 1130, 1323, 1522
FalI	AAGNNNNNCTT	2 cut(s)	1143, 1175
FaqI	GGGAC	4 cut(s)	1114, 1146, 1166, 1306
FatI	CATG	8 cut(s)	31, 366, 514, 583, 679, 1126, 1319, 1518
FblI	GTMKAC	2 cut(s)	982, 1509
Fnu4HI	GCNGC	5 cut(s)	21, 24, 430, 726, 1488
FokI	GGATG	5 cut(s)	108, 383, 467, 497, 1299
FriOI	GRGCYC	3 cut(s)	384, 390, 1309
Fsp4HI	GCNGC	5 cut(s)	21, 24, 430, 726, 1488
FspBI	CTAG	4 cut(s)	149, 1110, 1310, 1532
GluI	GCNGC	5 cut(s)	21, 24, 430, 726, 1488
GsaI	CCCAGC	2 cut(s)	474, 1286
GsuI	CTGGAG	2 cut(s)	741, 1432
HaeIII	GGCC	6 cut(s)	254, 266, 407, 953, 992, 1142
HapII	CCGG	6 cut(s)	107, 255, 281, 358, 502, 993
Hin1II	CATG	8 cut(s)	35, 370, 518, 587, 683, 1130, 1323, 1522
HinfI	GANTC	3 cut(s)	274, 1087, 1123
HpaII	CCGG	6 cut(s)	107, 255, 281, 358, 502, 993
HphI	GGTGA	1 cut(s)	656
Hpy166II	GTNNAC	4 cut(s)	58, 66, 983, 1510
Hpy188I	TCNGA	9 cut(s)	185, 246, 273, 309, 783, 854, 1075, 1346, 1402
Hpy188III	TCNNGA	9 cut(s)	112, 281, 350, 662, 752, 1127, 1250, 1302, 1411
Hpy8I	GTNNAC	4 cut(s)	58, 66, 983, 1510
Hpy99I	CGWCG	1 cut(s)	95
HpyCH4III	ACNGT	3 cut(s)	261, 576, 836
HpyCH4IV	ACGT	4 cut(s)	141, 545, 790, 1366
HpyCH4V	TGCA	9 cut(s)	102, 432, 707, 775, 1010, 1336, 1357, 1385, 1490
HpyF10VI	GCNNNNNNNGC	4 cut(s)	17, 445, 725, 1313
HpyF3I	CTNAG	2 cut(s)	243, 441
HpySE526I	ACGT	4 cut(s)	141, 545, 790, 1366
Hsp92II	CATG	8 cut(s)	35, 370, 518, 587, 683, 1130, 1323, 1522
Kpn2I	TCCGGA	1 cut(s)	280
KspI	CCGCGG	1 cut(s)	24
Kzo9I	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
LmnI	GCTCC	7 cut(s)	379, 445, 690, 722, 751, 1304, 1313
Lsp1109I	GCAGC	2 cut(s)	416, 1474
LweI	GCATC	3 cut(s)	202, 310, 445
MabI	ACCWGGT	1 cut(s)	1264
MaeI	CTAG	4 cut(s)	149, 1110, 1310, 1532
MaeII	ACGT	4 cut(s)	141, 545, 790, 1366
MaeIII	GTNAC	2 cut(s)	153, 1056
MalI	GATC	6 cut(s)	138, 248, 303, 756, 1041, 1536
MboI	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
MflI	RGATCY	3 cut(s)	301, 1039, 1534
MhlI	GDGCHC	4 cut(s)	384, 390, 423, 1309
MluCI	AATT	3 cut(s)	186, 202, 669
MlyI	GAGTC	1 cut(s)	1096
MmeI	TCCRAC	1 cut(s)	1017
MroI	TCCGGA	1 cut(s)	280
MseI	TTAA	3 cut(s)	923, 1106, 1220
MslI	CAYNNNNRTG	1 cut(s)	131
MspA1I	CMGCKG	3 cut(s)	23, 1282, 1487
MspI	CCGG	6 cut(s)	107, 255, 281, 358, 502, 993
MspR9I	CCNGG	3 cut(s)	502, 842, 1266
Mva1269I	GAATGC	1 cut(s)	432
MvaI	CCWGG	2 cut(s)	842, 1266
MvnI	CGCG	1 cut(s)	23
MwoI	GCNNNNNNNGC	4 cut(s)	17, 445, 725, 1313
NciI	CCSGG	1 cut(s)	502
NdeII	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
NlaIII	CATG	8 cut(s)	35, 370, 518, 587, 683, 1130, 1323, 1522
NlaIV	GGNNCC	5 cut(s)	124, 724, 804, 952, 1134
NspI	RCATGY	4 cut(s)	35, 518, 683, 1323
NspV	TTCGAA	1 cut(s)	190
PaeR7I	CTCGAG	1 cut(s)	383
PagI	TCATGA	1 cut(s)	1126
PaqCI	CACCTGC	2 cut(s)	698, 995
PciI	ACATGT	1 cut(s)	1319
PcsI	WCGNNNNNNNCGW	2 cut(s)	348, 1257
PctI	GAATGC	1 cut(s)	432
PfeI	GAWTC	2 cut(s)	274, 1123
PkrI	GCNGC	5 cut(s)	22, 25, 431, 727, 1489
PleI	GAGTC	1 cut(s)	1095
PmaCI	CACGTG	1 cut(s)	791
PmlI	CACGTG	1 cut(s)	791
PpsI	GAGTC	1 cut(s)	1095
Ppu21I	YACGTR	1 cut(s)	791
PpuMI	RGGWCCY	1 cut(s)	1133
PscI	ACATGT	1 cut(s)	1319
PsiI	TTATAA	1 cut(s)	542
Psp124BI	GAGCTC	3 cut(s)	384, 390, 1309
Psp1406I	AACGTT	2 cut(s)	545, 1366
Psp5II	RGGWCCY	1 cut(s)	1133
Psp6I	CCWGG	2 cut(s)	840, 1264
PspCI	CACGTG	1 cut(s)	791
PspFI	CCCAGC	2 cut(s)	470, 1282
PspGI	CCWGG	2 cut(s)	840, 1264
PspN4I	GGNNCC	5 cut(s)	124, 724, 804, 952, 1134
PspPI	GGNCC	6 cut(s)	122, 265, 951, 1133, 1140, 1268
PspPPI	RGGWCCY	1 cut(s)	1133
PspXI	VCTCGAGB	1 cut(s)	383
PstNI	CAGNNNCTG	2 cut(s)	1079, 1382
PsuI	RGATCY	3 cut(s)	301, 1039, 1534
PvuII	CAGCTG	2 cut(s)	1282, 1487
RsaI	GTAC	6 cut(s)	287, 293, 365, 736, 973, 1069
RsaNI	GTAC	6 cut(s)	286, 292, 364, 735, 972, 1068
RseI	CAYNNNNRTG	1 cut(s)	131
SacI	GAGCTC	3 cut(s)	384, 390, 1309
SacII	CCGCGG	1 cut(s)	24
SaqAI	TTAA	3 cut(s)	923, 1106, 1220
SatI	GCNGC	5 cut(s)	21, 24, 430, 726, 1488
Sau3AI	GATC	6 cut(s)	136, 246, 301, 754, 1039, 1534
Sau96I	GGNCC	6 cut(s)	122, 265, 951, 1133, 1140, 1268
SchI	GAGTC	1 cut(s)	1096
ScrFI	CCNGG	3 cut(s)	502, 842, 1266
SduI	GDGCHC	4 cut(s)	384, 390, 423, 1309
SexAI	ACCWGGT	1 cut(s)	1264
SfaNI	GCATC	3 cut(s)	202, 310, 445
SfcI	CTRYAG	3 cut(s)	714, 1435, 1538
Sfr274I	CTCGAG	1 cut(s)	383
Sfr303I	CCGCGG	1 cut(s)	24
SfuI	TTCGAA	1 cut(s)	190
SgrBI	CCGCGG	1 cut(s)	24
SinI	GGWCC	3 cut(s)	122, 1133, 1268
SlaI	CTCGAG	1 cut(s)	383
SmiMI	CAYNNNNRTG	1 cut(s)	131
SmlI	CTYRAG	1 cut(s)	383
SmoI	CTYRAG	1 cut(s)	383
Sse9I	AATT	3 cut(s)	186, 202, 669
SsiI	CCGC	8 cut(s)	21, 23, 69, 75, 81, 361, 394, 726
SspMI	CTAG	4 cut(s)	149, 1110, 1310, 1532
SstI	GAGCTC	3 cut(s)	384, 390, 1309
StyD4I	CCNGG	3 cut(s)	500, 840, 1264
StyI	CCWWGG	2 cut(s)	165, 297
TaaI	ACNGT	3 cut(s)	261, 576, 836
TaiI	ACGT	4 cut(s)	144, 548, 793, 1369
TaqI	TCGA	7 cut(s)	131, 190, 210, 249, 342, 384, 1197
TasI	AATT	3 cut(s)	186, 202, 669
TatI	WGTACW	2 cut(s)	285, 1067
TauI	GCSGC	3 cut(s)	23, 26, 728
TfiI	GAWTC	2 cut(s)	274, 1123
Tru1I	TTAA	3 cut(s)	923, 1106, 1220
Tru9I	TTAA	3 cut(s)	923, 1106, 1220
TscAI	CASTG	3 cut(s)	748, 1017, 1210
TseI	GCWGC	2 cut(s)	429, 1487
TspDTI	ATGAA	5 cut(s)	600, 674, 930, 1115, 1535
TspGWI	ACGGA	3 cut(s)	108, 1023, 1152
TspRI	CASTG	3 cut(s)	748, 1017, 1210
VpaK11BI	GGWCC	3 cut(s)	122, 1133, 1268
XagI	CCTNNNNNAGG	1 cut(s)	464
XapI	RAATTY	1 cut(s)	186
XceI	RCATGY	4 cut(s)	35, 518, 683, 1323
XhoI	CTCGAG	1 cut(s)	383
XmiI	GTMKAC	2 cut(s)	982, 1509
XspI	CTAG	4 cut(s)	149, 1110, 1310, 1532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.