Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005132

Physical Location & Seq

Genomic Coordinates Reverse (-)

15534099 .. 15544076

Gene Structure 9978 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1078100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4446 bp

ATGCAGACAAGCGATGACCATGAAGCAAAAGGTAATGTTAATGCAAACACTGGTTCATATAGTGATGGAATTGAGAACTCAAATTCTTTCGAAGATGATACAAATGCTGAGATATGGGAACCCCCTGAACCTGATGATCCAGAGGATGACATGGAGGGTAGTGTTGCTTTCAATGATGATGATGAAGAGGAATGTGGTGATGGCATGAAATGGGGTAAACCGAGTTCTTTGAGTCATTATAGAGATGAAGGAAGTGGGATTTATAGGTTTAAAGAGGAAAAACAAAGAGCAATGGAAGCAGTGATTAATGGAAAGTTTAAAGCCCTGATCAGTCAGCTACTTAAATCCGTGGGTGTTGCATCTTTGGGGGAGGGTGGTGAAAGTTGGGTGGACATTATTGCTTCTTTGTCATGGGAAGCTGCTTCATTTTTAAAACCTGATGCTGTTGTTGGTAAAGCAATGGATCCAGATGGCTACGTGAAAGTAAAATGCATCGCAACTGGTGTTCGCAGCCAAAGTCAACTAGTGAAAGGTTTGGTCTTCAAAAGGCATGCTGCACACAAGCATATGTCGACCAAGTACAAGAACCCAAGGTTTTTGCTAATCAAGGGAATACTTGGTCAGTCTTCAAGTGGATTGTCATCCTTTGATTCAATGGAACAGGAAAAAGGTTATCTGAAGTTTGTCATCGAAATGTTAGAACTGTGCCATCCAGATGTGATTTTAGTGGAAAAAACTGTTTCTCGTGATATTCAAGAGTCTATTCTTGACAAAGGAATGACACTGATCTTTGACATGAAGCTCCATCGCCTAGAGAGAGTTGCTCGTTGTACTGGATCACCAATTTTGTCATCAGATACAATGACGAGCGAAAAGCTAAAAAAGTGCGACTCTTTTCACATTGAAAAATTTAAAGAGGAACATGCTGGAGGTGGAGGAAAGGTCCCAAGTAAAACATTAATGTTTATTGAGGGCTGTCCTACACGCCTTGGCTGTACGGTTTTGTTGAAAGGAGCACAGAGTGATGAATTGAAGAAGGTCAAGTGTGTAGTGCAGTGTGCAGTTATTCTGGCGTATCATTTAATTCATGAGACTGCTTTCCTTGTTGACCAGAGAGCAATGTTCTCTACACTTCCATTTGCTGATGTAGAAACTGATTTGTCCACTGATAAAGAAACCCTTAATTTAGGATCTATTAACCCATGTGTTCATCAGAATACAGAGACTAATGCTGAAACTGGGTCTGATACAGTTGATATTCCCAGTTCCAATGGATTTAATGAAGGATGTTCCCACGATTCTACCTTGGAATTTGAAGGCCGTTCCACTTCCGATGCATCTTACAACCCTGCTGTACTTCCTGGCTTCTCGTCCATTTCAGCATCATTAAGGAAAGTTATAGGGGAGAATTTTCCTCAAGCATCTTCTTCTTATCAGTCTCTTTCTTCATACTTTGGATTCAGTGGAAGAGAACTGAATGACCCGATCACAAGATCTGTTTCTGCTTGTACAACTCCCGAGGCAACTGATCATTGTGATGTTGAAGATAAAGGTAGTTTCCACGAAGAGAGATCACTTAATGGGCATAATCAGACTTCATTTACATGCTCGGAAGCGTCTCTTGAGGTGAAAGAAAATGGTGGAAATAGTGAAGATCAAATGAAAAGTAAGAACCAAATCAGCACTGTGTTGGATTCTCAGAGTATTTTGGTTTTGATGTCTAGCCAAAATGCTTTAAGAGGCACTGTTTGTGAGCAGAGCCATTTTTCTCATATCATGTTCTACAAGAATTTCGATATCCCCATCGGAAAGTTTCTTCAAGATAATTTACTCAATCAGCAAAGCCAGTGCACATCATGTGGTGAACTTCCAGAAGCTCACTTCTACTATTATGCACATCGTAACAAGCAGCTTACTATCCGAGTTAAACGGCTCCCTGGAGAAGTGCATTTGCCTGGTGAAGCAGAAGGGAAACTTTGGATGTGGAGTCGATGTGGTAAATGTAAATCTAGGAATGGAATCTCAAAGTGTACAAAAAGAGTATTGATTTCCACTTCTGCTCGTTGTCTATCATTTGGAAATTTCCTGGAGCTCAATTTCTCGAACCCATCTTTGTCTAATTTATTTTCTAGCTGCGGCCATTCTTTGCAGAAGGACTTTCTCTACTTCTTTGGATTAGGCCCTATGGTTGCTCTGTTCAAGTATTCCCCCGTCACGACTTATACTGTTTCTGTGCCTCCTCTGAAGCTTCCGTTCAGCAGTTCAATTAGACAAGACTGGCTTATGAAAGAAACTCAGAATGTGTACATGAAATTGATTTACTTGTTCAAAGAGGTTGCCAACTCTTTAGAGAAGATGAGATCTCAGTTTGATGGGTTGACTTTGAAACTTCGAGGCTCATTTAAAGAATTTTCTGACATCGAAGAGATGCTAAAGCAAGAAAAATCTGAGTTCGAGGTAGTGCTCAAGAATGCTGTTACCAAGACTGAGAACTTGGACCAGGCTGCCTACAAACTACTTAGCTTGAACAGAGTACTGTGGGAACTGCTCCTTGAAGCATGCATTTGGGAACAGCGCTTGCATTCATTACTGTCACTAGATCCTATGATGATCCATTCTAGAGCTTCTGAGAAGGTAGAGCCAGAAAAAGTTGATTCGGATATTGGTATCAAATTAGAAGTCAAATTGGATACAGCTGCAGATGCTGATGGATCTCCAATAGAAGACATTCCAATTGAAGGTCCTGTTCAAGAATCTAATGGAGCTGATCCAGTTGATGTGTCCGATTTGGCTGAGGGTTTCAAAACTCCGAATGTGGATGGTTCAAGCCCAAAACGGTTGGCCAGGCAGGGGTCCAATCTGAGAAATGGCTCCAGCTATGATCATTGTGAGAATGATCAGCTAGCAGAAAATGTGGATTGTGGTGCTTTTGCTGATTCAAATCTATCAATGAAAGGTACTTATTACCGTTCACTATCATTCGACTTGGAAGGTTCACATGACTGGTTTTGGGCACCGTTCTCCGAAATTCGTCAAGTTGGCGTGAGAGATCTACAGAGAGTGTTCTTTTCAAAGTTTGAAACAATTAGTAGCTATACTGCAGAGCACTTACCCACAGCGTATCAGCTGATCACGGAAGAAGGGAAAATGCTGCACATTCCTCTTGGAACTGATAATCATATTGTGTCAGACTATGAAGGAGAACTGTCGAGTATGATTGCTTGTGCACTGGCCATATTGAAAGATCTACCTTTACAAACAGAGGTTGTTGCTGACGATGGCAAGGGAGAGAGTGGAGTTGCTGCGAGAACATTTGAGCATTTACACAGTCTGACTCGACTTCCCACTAATACTACCCTGCATTGGTCTTCAAATGGTTCGTTAGATTTAGACTCTGTCCATAATATGGCAAGCATTTCTTCAGATGAGTCGCGCTTCTCCAGTTTTGATGGGTTGAATTTGTTGGATTCTCTGGTTTCGCCTGGAACTGTTAACCCAGTAGTCCCTGTTGGAGTTTCAAAATCACTTGGCAAGGATAAATATACTGTAATATGTCCATATGCCAATCAATTTCGTGATCTCCGCAATCGATGCTTCCAATCTGAAGTTGATTATATTGCTTCCCTTAGCCGTTGCAGAAACTGGGATGCTAAAGGTGGGAAGAGCAAATCCTTTTTTGCCAAAACACTTGATGACAGGTTGATAATTAAAGAAATCAAGAAGACAGAATTTGAGTCATTTATGAAGTTTTCTGATGAGTATTTCAAGCACATAAAGCTTTCTTTTGACAATGGGAATCAAACATGCCTTGCGAAAGTTCTTGGGATTTATCAGGTGATTGTGAGACAGACAAAAAGTGGGAAAGAGATGAGGCATGATCTGATGGTCATGGAGAATCTTACCTTTGGTCGGAACATCACTCGCCAGTATGATCTGAAAGGTGCTCTACATGCTCGATTCAATTCTGCTACTGATGGTTCCGGAGAGGTTCTTTTGGATCAGAACTTTGTCAATGACATGAACTCATCTCCACTGTATGTTAGTAACAAAGCAAAGCGTATATTGCAGCGGGCTGTGTGGAATGACACAACTTTCCTCAATTCAATCAATGTCATGGATTATTCATTGCTTGTGGGAGTAGATACTGAACGCCGTGAGCTTGTATGTGGCATTATTGATTATCTTAGGCAGTATACATGGGACAAGCAACTAGAGACATGGGTGAAGTCTTCACTTGTGCCAAAAAATGTCTTGCCGACTGTCATCTCTCCAAAAGAGTACAAGAGGCGATTCAGAAAGTTCATGTCTAAGCATTTCTTGAGCGTCCCTGATGATTGGTGCTCACCAGAATCCTCTGACCCTTGTCACCAATGTGCTGTTGGAAATGATGATTCATCTCATTCAAAATCCCATAGGAAAGGCGGATTAAACAATTTGTCAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

1482

Amino Acids

165.27

Weight (kDa)

5.57

Isoelectric Point (pI)

41.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	111 - 367	1.9e-19	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1219 - 1384	1.6e-33	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1386 - 1437	3.5e-09	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34260 AT1G34260 AT1G34260 AT1G34260
fragaria_vesca	FvH4_2g12440 FvH4_2g12440 FvH4_2g12440 FvH4_2g12440 FvH4_2g12440
malus_domestica	MD00G1078100.v1.1 MD10G1044000.v1.1 MD10G1044100.v1.1
prunus_persica	Prupe.8G050600_v2.0.a1
pyrus_communis	pycom05g03120 pycom05g03130 pycom10g03060 pycom10g03070 pycom10g03080
rosa_chinensis	RchiOBHm_Chr6g0272731
rosa_laevigata	RLG00000013661
rosa_multiflora	Rmu_co8449107.1_g000001 Rmu_sc0003080.1_g000001 Rmu_sc0014453.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196050
rosa_rugosa	Rorug06G0070400
rosa_samantha	Rh6AG186600 Rh6BG189900 Rh6CG188700 Rh6DG180100
rosa_wichuraiana	Rw6G016100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	3013
AccB7I	CCANNNNNTGG	1 cut(s)	3406
AccI	GTMKAC	2 cut(s)	572, 4193
AccII	CGCG	1 cut(s)	3433
AccIII	TCCGGA	1 cut(s)	3980
AciI	CCGC	4 cut(s)	2136, 3583, 4069, 4422
AcoI	YGGCCR	3 cut(s)	2137, 2841, 3231
AcuI	CTGAAG	4 cut(s)	698, 2264, 3405, 3624
AdeI	CACNNNGTG	1 cut(s)	1022
AfeI	AGCGCT	1 cut(s)	2576
AfiI	CCNNNNNNNGG	5 cut(s)	2738, 2850, 3363, 3406, 3909
AhlI	ACTAGT	1 cut(s)	523
AjnI	CCWGG	7 cut(s)	1360, 1936, 1954, 2085, 2499, 2843, 3481
AleI	CACNNNNGTG	1 cut(s)	4371
Alw21I	GWGCWC	8 cut(s)	1018, 1853, 2094, 2466, 3108, 3229, 3946, 4343
Alw26I	GTCTC	6 cut(s)	1085, 1217, 1443, 1623, 3838, 4208
Alw44I	GTGCAC	2 cut(s)	1849, 3225
AlwNI	CAGNNNCTG	2 cut(s)	2705, 3642
Ama87I	CYCGRG	1 cut(s)	1517
Aor13HI	TCCGGA	1 cut(s)	3980
Aor51HI	AGCGCT	1 cut(s)	2576
AoxI	GGCC	5 cut(s)	1318, 2137, 2179, 2841, 3231
ApaLI	GTGCAC	2 cut(s)	1849, 3225
ArsI	GACNNNNNNTTYG	2 cut(s)	2664, 2696
AseI	ATTAAT	2 cut(s)	306, 959
Asp700I	GAANNNNTTC	1 cut(s)	2727
AspLEI	GCGC	2 cut(s)	2577, 3435
AspS9I	GGNCC	5 cut(s)	943, 2180, 2497, 2741, 2853
AsuII	TTCGAA	1 cut(s)	90
AsuNHI	GCTAGC	1 cut(s)	2902
AvaI	CYCGRG	1 cut(s)	1517
AvaII	GGWCC	4 cut(s)	943, 2497, 2741, 2853
BaeGI	GKGCMC	3 cut(s)	1853, 3016, 3229
BalI	TGGCCA	2 cut(s)	2843, 3233
BamHI	GGATCC	1 cut(s)	463
BanI	GGYRCC	1 cut(s)	3013
BanII	GRGCYC	1 cut(s)	2094
BauI	CACGAG	1 cut(s)	744
BbsI	GAAGAC	6 cut(s)	532, 618, 2730, 3360, 3728, 4221
Bbv12I	GWGCWC	8 cut(s)	1018, 1853, 2094, 2466, 3108, 3229, 3946, 4343
BbvCI	CCTCAGC	1 cut(s)	2793
BceAI	ACGGC	4 cut(s)	1305, 1946, 3615, 4137
BciT130I	CCWGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
BciVI	GTATCC	1 cut(s)	2683
BclI	TGATCA	5 cut(s)	327, 1528, 2881, 2896, 3129
BcoDI	GTCTC	6 cut(s)	1085, 1217, 1443, 1623, 3838, 4208
BcuI	ACTAGT	1 cut(s)	523
BfaI	CTAG	9 cut(s)	524, 812, 1722, 2010, 2130, 2597, 2619, 2903, 4211
BfmI	CTRYAG	3 cut(s)	2697, 3053, 3099
BfoI	RGCGCY	1 cut(s)	2578
BfuI	GTATCC	1 cut(s)	2683
BglII	AGATCT	4 cut(s)	1493, 2360, 3049, 3244
BmcAI	AGTACT	1 cut(s)	2535
Bme1390I	CCNGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
Bme18I	GGWCC	4 cut(s)	943, 2497, 2741, 2853
BmeT110I	CYCGRG	1 cut(s)	1517
BmgT120I	GGNCC	5 cut(s)	943, 2180, 2497, 2741, 2853
BmiI	GGNNCC	8 cut(s)	120, 465, 945, 1934, 2854, 2872, 3015, 3979
BmrFI	CCNGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
BmrI	ACTGGG	4 cut(s)	1248, 1257, 3491, 3652
BmtI	GCTAGC	1 cut(s)	2906
BmuI	ACTGGG	4 cut(s)	1248, 1257, 3491, 3652
BoxI	GACNNNNGTC	1 cut(s)	4362
BpiI	GAAGAC	6 cut(s)	532, 618, 2730, 3360, 3728, 4221
BplI	GAGNNNNNCTC	2 cut(s)	808, 840
BpmI	CTGGAG	5 cut(s)	948, 1959, 2108, 2857, 3424
Bpu10I	CCTNAGC	2 cut(s)	2793, 3626
Bpu14I	TTCGAA	1 cut(s)	90
BpuEI	CTTGAG	4 cut(s)	1401, 1643, 2450, 4339
Bsa29I	ATCGAT	1 cut(s)	3589
BsaAI	YACGTR	1 cut(s)	478
BsaBI	GATNNNNATC	2 cut(s)	640, 2940
BsaJI	CCNNGG	6 cut(s)	348, 590, 988, 1305, 1518, 1936
BsaWI	WCCGGW	1 cut(s)	3980
BsaXI	ACNNNNNCTCC	4 cut(s)	927, 957, 1978, 2008
Bsc4I	CCNNNNNNNGG	5 cut(s)	2738, 2850, 3363, 3406, 3909
Bse3DI	GCAATG	4 cut(s)	297, 465, 1125, 4124
Bse8I	GATNNNNATC	2 cut(s)	640, 2940
BseAI	TCCGGA	1 cut(s)	3980
BseBI	CCWGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
BseCI	ATCGAT	1 cut(s)	3589
BseDI	CCNNGG	6 cut(s)	348, 590, 988, 1305, 1518, 1936
BseGI	GGATG	7 cut(s)	151, 641, 709, 1292, 1986, 2824, 3652
BseJI	GATNNNNATC	2 cut(s)	640, 2940
BseLI	CCNNNNNNNGG	5 cut(s)	2738, 2850, 3363, 3406, 3909
BseMI	GCAATG	4 cut(s)	297, 465, 1125, 4124
BseMII	CTCAG	9 cut(s)	99, 1712, 2309, 2378, 2439, 2478, 2619, 2784, 2852
BseRI	GAGGAG	1 cut(s)	2229
BseSI	GKGCMC	3 cut(s)	1853, 3016, 3229
BsgI	GTGCAG	4 cut(s)	540, 1073, 1080, 3137
Bsh1236I	CGCG	1 cut(s)	3433
BshFI	GGCC	5 cut(s)	1320, 2139, 2181, 2843, 3233
BshNI	GGYRCC	1 cut(s)	3013
BshVI	ATCGAT	1 cut(s)	3589
BsiHKAI	GWGCWC	8 cut(s)	1018, 1853, 2094, 2466, 3108, 3229, 3946, 4343
BsiHKCI	CYCGRG	1 cut(s)	1517
BsiSI	CCGG	1 cut(s)	3981
BslFI	GGGAC	4 cut(s)	929, 3488, 4214, 4310
BslI	CCNNNNNNNGG	5 cut(s)	2738, 2850, 3363, 3406, 3909
BsmAI	GTCTC	6 cut(s)	1085, 1217, 1443, 1623, 3838, 4208
BsmBI	CGTCTC	1 cut(s)	1623
BsmFI	GGGAC	4 cut(s)	929, 3488, 4214, 4310
BsmI	GAATGC	2 cut(s)	2476, 2581
BsnI	GGCC	5 cut(s)	1320, 2139, 2181, 2843, 3233
BsoBI	CYCGRG	1 cut(s)	1517
Bsp119I	TTCGAA	1 cut(s)	90
Bsp1286I	GDGCHC	9 cut(s)	1018, 1853, 2094, 2466, 3016, 3108, 3229, 3946, 4343
Bsp13I	TCCGGA	1 cut(s)	3980
Bsp1407I	TGTACA	3 cut(s)	1508, 2030, 2304
BspACI	CCGC	4 cut(s)	2136, 3583, 4069, 4422
BspANI	GGCC	5 cut(s)	1320, 2139, 2181, 2843, 3233
BspCNI	CTCAG	9 cut(s)	100, 1711, 2308, 2377, 2440, 2479, 2620, 2785, 2853
BspDI	ATCGAT	1 cut(s)	3589
BspEI	TCCGGA	1 cut(s)	3980
BspFNI	CGCG	1 cut(s)	3433
BspHI	TCATGA	1 cut(s)	1087
BspLI	GGNNCC	8 cut(s)	120, 465, 945, 1934, 2854, 2872, 3015, 3979
BspMAI	CTGCAG	2 cut(s)	2701, 3103
BspOI	GCTAGC	1 cut(s)	2906
BspQI	GCTCTTC	1 cut(s)	3656
BspT104I	TTCGAA	1 cut(s)	90
BspT107I	GGYRCC	1 cut(s)	3013
BsrDI	GCAATG	4 cut(s)	297, 465, 1125, 4124
BsrGI	TGTACA	3 cut(s)	1508, 2030, 2304
BssECI	CCNNGG	6 cut(s)	348, 590, 988, 1305, 1518, 1936
BssNAI	GTATAC	1 cut(s)	4194
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	3 cut(s)	590, 988, 1305
Bst1107I	GTATAC	1 cut(s)	4194
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
Bst6I	CTCTTC	5 cut(s)	180, 1462, 1560, 2418, 3656
BstAPI	GCANNNNNTGC	1 cut(s)	3591
BstAUI	TGTACA	3 cut(s)	1508, 2030, 2304
BstBAI	YACGTR	1 cut(s)	478
BstBI	TTCGAA	1 cut(s)	90
BstC8I	GCNNGC	6 cut(s)	552, 2560, 2579, 2904, 3412, 4071
BstDSI	CCRYGG	1 cut(s)	348
BstF5I	GGATG	7 cut(s)	151, 641, 709, 1292, 1986, 2824, 3652
BstFNI	CGCG	1 cut(s)	3433
BstH2I	RGCGCY	1 cut(s)	2578
BstHHI	GCGC	2 cut(s)	2577, 3435
BstMAI	GTCTC	6 cut(s)	1085, 1217, 1443, 1623, 3838, 4208
BstMWI	GCNNNNNNNGC	6 cut(s)	296, 2702, 3591, 3775, 3950, 4063
BstNI	CCWGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
BstNSI	RCATGY	6 cut(s)	554, 926, 1608, 2562, 3807, 3953
BstPAI	GACNNNNGTC	1 cut(s)	4362
BstSCI	CCNGG	7 cut(s)	1360, 1936, 1954, 2085, 2499, 2843, 3481
BstSFI	CTRYAG	3 cut(s)	2697, 3053, 3099
BstSLI	GKGCMC	3 cut(s)	1853, 3016, 3229
BstUI	CGCG	1 cut(s)	3433
BstV2I	GAAGAC	6 cut(s)	532, 618, 2730, 3360, 3728, 4221
BstX2I	RGATCY	8 cut(s)	463, 1190, 1493, 2360, 2599, 2711, 3049, 3244
BstYI	RGATCY	8 cut(s)	463, 1190, 1493, 2360, 2599, 2711, 3049, 3244
BstZ17I	GTATAC	1 cut(s)	4194
Bsu15I	ATCGAT	1 cut(s)	3589
BsuI	GTATCC	1 cut(s)	2683
BsuRI	GGCC	5 cut(s)	1320, 2139, 2181, 2843, 3233
BsuTUI	ATCGAT	1 cut(s)	3589
BtgI	CCRYGG	1 cut(s)	348
BtgZI	GCGATG	3 cut(s)	27, 478, 791
BtsCI	GGATG	7 cut(s)	151, 641, 709, 1292, 1986, 2824, 3652
BtsI	GCAGTG	2 cut(s)	306, 1061
Cac8I	GCNNGC	6 cut(s)	552, 2560, 2579, 2904, 3412, 4071
CaiI	CAGNNNCTG	2 cut(s)	2705, 3642
CciI	TCATGA	1 cut(s)	1087
CfoI	GCGC	2 cut(s)	2577, 3435
Cfr13I	GGNCC	5 cut(s)	943, 2180, 2497, 2741, 2853
ClaI	ATCGAT	1 cut(s)	3589
CseI	GACGC	2 cut(s)	1605, 4312
DraI	TTTAAA	5 cut(s)	271, 319, 432, 913, 2404
DraIII	CACNNNGTG	1 cut(s)	1022
EaeI	YGGCCR	3 cut(s)	2137, 2841, 3231
Eam1104I	CTCTTC	5 cut(s)	180, 1462, 1560, 2418, 3656
EarI	CTCTTC	5 cut(s)	180, 1462, 1560, 2418, 3656
EciI	GGCGGA	1 cut(s)	4437
Ecl136II	GAGCTC	1 cut(s)	2092
Eco130I	CCWWGG	3 cut(s)	590, 988, 1305
Eco24I	GRGCYC	1 cut(s)	2094
Eco32I	GATATC	1 cut(s)	1798
Eco47I	GGWCC	4 cut(s)	943, 2497, 2741, 2853
Eco47III	AGCGCT	1 cut(s)	2576
Eco53kI	GAGCTC	1 cut(s)	2092
Eco57I	CTGAAG	4 cut(s)	698, 2264, 3405, 3624
Eco88I	CYCGRG	1 cut(s)	1517
EcoICRI	GAGCTC	1 cut(s)	2092
EcoO109I	RGGNCCY	3 cut(s)	943, 2180, 2741
EcoRII	CCWGG	7 cut(s)	1360, 1936, 1954, 2085, 2499, 2843, 3481
EcoRV	GATATC	1 cut(s)	1798
EcoT14I	CCWWGG	3 cut(s)	590, 988, 1305
EcoT22I	ATGCAT	3 cut(s)	494, 1339, 2564
EcoT38I	GRGCYC	1 cut(s)	2094
ErhI	CCWWGG	3 cut(s)	590, 988, 1305
Esp3I	CGTCTC	1 cut(s)	1623
FaqI	GGGAC	4 cut(s)	929, 3488, 4214, 4310
FauI	CCCGC	1 cut(s)	4062
FauNDI	CATATG	2 cut(s)	567, 3559
FbaI	TGATCA	5 cut(s)	327, 1528, 2881, 2896, 3129
FblI	GTMKAC	2 cut(s)	572, 4193
FokI	GGATG	7 cut(s)	158, 628, 696, 1299, 1993, 2831, 3659
FriOI	GRGCYC	1 cut(s)	2094
FspBI	CTAG	9 cut(s)	524, 812, 1722, 2010, 2130, 2597, 2619, 2903, 4211
GlaI	GCGC	2 cut(s)	2576, 3434
GsuI	CTGGAG	5 cut(s)	948, 1959, 2108, 2857, 3424
HaeII	RGCGCY	1 cut(s)	2578
HaeIII	GGCC	5 cut(s)	1320, 2139, 2181, 2843, 3233
HapII	CCGG	1 cut(s)	3981
HgaI	GACGC	2 cut(s)	1605, 4312
HhaI	GCGC	2 cut(s)	2577, 3435
Hin6I	GCGC	2 cut(s)	2575, 3433
HinP1I	GCGC	2 cut(s)	2575, 3433
HincII	GTYRAC	5 cut(s)	521, 573, 1108, 2379, 3493
HindII	GTYRAC	5 cut(s)	521, 573, 1108, 2379, 3493
HindIII	AAGCTT	2 cut(s)	2246, 3776
HpaI	GTTAAC	1 cut(s)	3493
HpaII	CCGG	1 cut(s)	3981
HpyCH4IV	ACGT	1 cut(s)	477
HpyF10VI	GCNNNNNNNGC	6 cut(s)	296, 2702, 3591, 3775, 3950, 4063
HpySE526I	ACGT	1 cut(s)	477
HspAI	GCGC	2 cut(s)	2575, 3433
Kpn2I	TCCGGA	1 cut(s)	3980
Ksp22I	TGATCA	5 cut(s)	327, 1528, 2881, 2896, 3129
KspAI	GTTAAC	1 cut(s)	3493
LguI	GCTCTTC	1 cut(s)	3656
LmnI	GCTCC	7 cut(s)	807, 1013, 1938, 2089, 2553, 2762, 2876
MaeI	CTAG	9 cut(s)	524, 812, 1722, 2010, 2130, 2597, 2619, 2903, 4211
MaeII	ACGT	1 cut(s)	477
MaeIII	GTNAC	6 cut(s)	1901, 2212, 2476, 2592, 4043, 4364
MfeI	CAATTG	1 cut(s)	2733
MflI	RGATCY	8 cut(s)	463, 1190, 1493, 2360, 2599, 2711, 3049, 3244
MhlI	GDGCHC	9 cut(s)	1018, 1853, 2094, 2466, 3016, 3108, 3229, 3946, 4343
MlsI	TGGCCA	2 cut(s)	2843, 3233
MluNI	TGGCCA	2 cut(s)	2843, 3233
MlyI	GAGTC	8 cut(s)	241, 767, 884, 1996, 3328, 3386, 3437, 3743
MmeI	TCCRAC	5 cut(s)	1671, 3444, 3490, 3890, 4360
Mox20I	TGGCCA	2 cut(s)	2843, 3233
Mph1103I	ATGCAT	3 cut(s)	494, 1339, 2564
MroI	TCCGGA	1 cut(s)	3980
MroXI	GAANNNNTTC	1 cut(s)	2727
MscI	TGGCCA	2 cut(s)	2843, 3233
MslI	CAYNNNNRTG	5 cut(s)	692, 714, 1536, 1603, 4371
Msp20I	TGGCCA	2 cut(s)	2843, 3233
MspA1I	CMGCKG	3 cut(s)	2696, 3127, 4069
MspI	CCGG	1 cut(s)	3981
MspR9I	CCNGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
MunI	CAATTG	1 cut(s)	2733
Mva1269I	GAATGC	2 cut(s)	2476, 2581
MvaI	CCWGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
MvnI	CGCG	1 cut(s)	3433
MwoI	GCNNNNNNNGC	6 cut(s)	296, 2702, 3591, 3775, 3950, 4063
NdeI	CATATG	2 cut(s)	567, 3559
NheI	GCTAGC	1 cut(s)	2902
NlaIV	GGNNCC	8 cut(s)	120, 465, 945, 1934, 2854, 2872, 3015, 3979
NmuCI	GTSAC	3 cut(s)	2212, 2592, 4364
NsiI	ATGCAT	3 cut(s)	494, 1339, 2564
NspI	RCATGY	6 cut(s)	554, 926, 1608, 2562, 3807, 3953
NspV	TTCGAA	1 cut(s)	90
OliI	CACNNNNGTG	1 cut(s)	4371
PaeI	GCATGC	2 cut(s)	554, 2562
PagI	TCATGA	1 cut(s)	1087
PciSI	GCTCTTC	1 cut(s)	3656
PctI	GAATGC	2 cut(s)	2476, 2581
PdmI	GAANNNNTTC	1 cut(s)	2727
PflFI	GACNNNGTC	1 cut(s)	3395
PflMI	CCANNNNNTGG	1 cut(s)	3406
PfoI	TCCNGGA	1 cut(s)	2085
PleI	GAGTC	8 cut(s)	240, 766, 884, 1995, 3328, 3386, 3436, 3742
PpsI	GAGTC	8 cut(s)	240, 766, 884, 1995, 3328, 3386, 3436, 3742
Ppu21I	YACGTR	1 cut(s)	478
PpuMI	RGGWCCY	2 cut(s)	943, 2741
PshAI	GACNNNNGTC	1 cut(s)	4362
PshBI	ATTAAT	2 cut(s)	306, 959
Psp124BI	GAGCTC	1 cut(s)	2094
Psp5II	RGGWCCY	2 cut(s)	943, 2741
Psp6I	CCWGG	7 cut(s)	1360, 1936, 1954, 2085, 2499, 2843, 3481
PspGI	CCWGG	7 cut(s)	1360, 1936, 1954, 2085, 2499, 2843, 3481
PspN4I	GGNNCC	8 cut(s)	120, 465, 945, 1934, 2854, 2872, 3015, 3979
PspPI	GGNCC	5 cut(s)	943, 2180, 2497, 2741, 2853
PspPPI	RGGWCCY	2 cut(s)	943, 2741
PsrI	GAACNNNNNNTAC	2 cut(s)	208, 240
PstI	CTGCAG	2 cut(s)	2701, 3103
PstNI	CAGNNNCTG	2 cut(s)	2705, 3642
PsuI	RGATCY	8 cut(s)	463, 1190, 1493, 2360, 2599, 2711, 3049, 3244
PsyI	GACNNNGTC	1 cut(s)	3395
PvuII	CAGCTG	2 cut(s)	2696, 3127
RseI	CAYNNNNRTG	5 cut(s)	692, 714, 1536, 1603, 4371
SacI	GAGCTC	1 cut(s)	2094
SalI	GTCGAC	1 cut(s)	571
SapI	GCTCTTC	1 cut(s)	3656
Sau96I	GGNCC	5 cut(s)	943, 2180, 2497, 2741, 2853
ScaI	AGTACT	1 cut(s)	2535
SchI	GAGTC	8 cut(s)	241, 767, 884, 1996, 3328, 3386, 3437, 3743
ScrFI	CCNGG	7 cut(s)	1362, 1938, 1956, 2087, 2501, 2845, 3483
SduI	GDGCHC	9 cut(s)	1018, 1853, 2094, 2466, 3016, 3108, 3229, 3946, 4343
SfcI	CTRYAG	3 cut(s)	2697, 3053, 3099
SfuI	TTCGAA	1 cut(s)	90
SinI	GGWCC	4 cut(s)	943, 2497, 2741, 2853
SmiMI	CAYNNNNRTG	5 cut(s)	692, 714, 1536, 1603, 4371
SmlI	CTYRAG	4 cut(s)	1416, 1622, 2465, 4318
SmoI	CTYRAG	4 cut(s)	1416, 1622, 2465, 4318
SpeI	ACTAGT	1 cut(s)	523
SphI	GCATGC	2 cut(s)	554, 2562
SsiI	CCGC	4 cut(s)	2136, 3583, 4069, 4422
SspMI	CTAG	9 cut(s)	524, 812, 1722, 2010, 2130, 2597, 2619, 2903, 4211
SstI	GAGCTC	1 cut(s)	2094
StyD4I	CCNGG	7 cut(s)	1360, 1936, 1954, 2085, 2499, 2843, 3481
StyI	CCWWGG	3 cut(s)	590, 988, 1305
TaiI	ACGT	1 cut(s)	480
TatI	WGTACW	8 cut(s)	579, 830, 1354, 1508, 2030, 2304, 2533, 4278
TauI	GCSGC	1 cut(s)	2139
TseFI	GTSAC	3 cut(s)	2212, 2592, 4364
Tsp45I	GTSAC	3 cut(s)	2212, 2592, 4364
TspGWI	ACGGA	3 cut(s)	337, 2241, 3149
Tth111I	GACNNNGTC	1 cut(s)	3395
Van91I	CCANNNNNTGG	1 cut(s)	3406
VneI	GTGCAC	2 cut(s)	1849, 3225
VpaK11BI	GGWCC	4 cut(s)	943, 2497, 2741, 2853
VspI	ATTAAT	2 cut(s)	306, 959
XbaI	TCTAGA	1 cut(s)	2618
XceI	RCATGY	6 cut(s)	554, 926, 1608, 2562, 3807, 3953
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4376
XmiI	GTMKAC	2 cut(s)	572, 4193
XmnI	GAANNNNTTC	1 cut(s)	2727
XspI	CTAG	9 cut(s)	524, 812, 1722, 2010, 2130, 2597, 2619, 2903, 4211
ZrmI	AGTACT	1 cut(s)	2535
Zsp2I	ATGCAT	3 cut(s)	494, 1339, 2564

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.