Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005132

Physical Location & Seq

Genomic Coordinates Forward (+)

5932591 .. 5942574

Gene Structure 9984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1044100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4827 bp

ATGTGTCACTACTGTGGTGCAGAATTGACACAGTCCAAGGAAAACAAGAAAAAACTGGAGAATTCGAAATTATTTCCATTAGCTAAAGGAGGCCCCAGAAAGTATTGCAAATTTTGTGCGGAGAAACAGGAGCGAGAATCTATCAAGCAGGGTAATACGATTTTATATACAATGCCAATGATCAGTCCAGCAACTTCCTTGTCAAGCAGTGACAGTTGTGTCTCTAGCTGGAGTGAGTTTTCTGTTGATGTGAGCTCATGCGATAGGGGTATTCAAGAGGAAAACACGACAGGCGGTTCTCAAGAGGATCTCTTTTGTCGGTCTAATGGGAGGTTACAAAACTCAAGTTTTGAACATCCAGTAAATGGATTTGATAGATCAGAAAAACAGATGGAAAACAACCTGAGCGAAAGTAGTTGTGGCAGTGATGTGTATACAGTAAGAGATGTTGAGATTATACAGACAAGTGATGACCATGAAGCAAAAGCTAATGTCAATGCAAGCACTAGTTCACATAGTGATGGAGCTGAGAACTCAAATTCTTTAGAAGATGATACAAATGCTGAGATATGGGAACCCCCTGAACCTGATGATCCAGAGGATGACATGGAGGGTAGTGTTGCTTTCAATGATGATGACGATGAGGAATGTGGTGATGGCATGAAATGGGGTACACCGAGTTCTTTGAGTCATTCTGGAGATGAAGGAAGGGGAAGTTATACGTTTAAAGAGGAAAAACAAAGAGCAATGGAAGCGGTGATTAATGGAAAGTTTAAAGCCTTGATCAGTCAGCTACTTAAATCCGTGGGTGTTGCATCTTTGGGGGAGGGTGGTGAAAGTTGGGTGGACATTATTGCTTCTTTGTCATGGGAAGCTGCTTCATTTTTGAAACCTGATGCTGTTGTTGGTAAAGCAATGGATCCAGATGGCTACGTGAAAGTAAAATGCATTGCAACTGGTGTTCGCACCCAAAGTCAACTAGTAAAAGGTTTGGTCTTCAAAAAGCATGCTGCACACAAACATATGTCGACCAAGTACAAGAATCCAAGGTTGTTGCTAATCAAGGGAGTACTTGGTCTGTCTTCAAGTGGATTGTCATCCTTTGATTCAATGGAACAGGAAAGAGATTATCTGAAGTTTGTTATCGAAATGTTAGAATTGTGCCATCCAAATGTGGTTTTAGTGGAAAAAACTGTTTCTCGTGATATTCAAGAGGCTATTCTTGGGAATGGAATTACACTGATCTTTGACATGAAGCTCCATCGCCTGGAGAGAGTTGCTCGTTGTACTGGATCACCAATTTTATCATCAGATACAATGACGAGCAAAAAACTAAAACAGTGCGACTCTTTTCACATTGAAAAGTTTATAGAGGAGCATGCTGGAGGTGGAGGAAAGGTCCCAAGTAAAACATTAATGTTTATTGAGGGCTGTCCTACACGCCTGGGCTGTACGATTTTGCTGAAAGGAGCACGGAGTGATGAATTGAAGAAGGTCAAGTGTGTTGTGCAGTGTGCAGTTATTCTGGCGTATCATTTAATGCTCGAGACTGCTTTCCTTGTTGACCAGAGAGCAATGTTCTCTACACTTCCATTAGCTGATGTAGCAATGGATTTGTCCACTGATAAAGAAACCCTTAATTTAGGATCAATTAACTCATGTGTCCATCAGAATACAGAGACTAGTGCTGAAACTGGGTCTGATACAGTTGATATTCCCATTTCCAATGGATTTCATGAAGGATGTTCCCACAGTTCTACCTTGGACGTCGAAGGCTGTTCCACATTCGATGAACCATACAATCCTGCTGTACTTTCTAGCTTGTCGTCCATTTCAGCATCATTAAGGAAAGTTATAGGGGAGAATTTTCCCCTAGCATCTTCTTCTTATCAGTCTCTTTCTTCATACTTTGGATTCAGTGGAAGAGAACTGAATGACCCAATCACAAGATCTGTTTCCGTTTGTACAACTCCTGAGGAAACTGAGCATTGTGATGTTGAAGATAAAGGTAGTGTTGAAGAAGAGAGATCACTTAATGGGAAAAATAAGACTTCATTTAAATACACAGAAGCTTCTCTTGAGGTGAAAGAAGATGATGGAAATAGTGAAGATCAAATGAAAAGTAAGAACCAAATCAGTGCTGTGTTGGATTCTCAGAGTATTTTGGTTTTGATGTCTAGCCAGAATGCATTAAGAGGCACTGTTTGTGAGCAGAGCCGTTTTTCTCATATCATGTTCTACAAGAATTTCGATGTTCCCATTGGAAAGTTTCTTCAAGATAATTTGCTCAATCAGCAAAGCCAGTGCACATCATGTGGTGGACTTCCAGAAGCTCACTTCTACTATTATGCACATCGTAATAAGCAGCTTACTATTCGAGTTAAACGTCTCCCTGGAGAAGTGCATTTGCCCGGTGGAGCAGAAGGGAAACTTTGGATGTGGAGTCGATGTGGTAAATGTAAATCTAGGAATGGAATCTCGAAGTGTACAAAAAGAGTATTGATTTCCACTTCTGCACGTGGTCTATCATTTGGAAATTTCCTGGAGCTCAATTTCTCGAAGCCATCTTTGTCTAATTTATTTTCTAGCTGCGGCCATTCTTTGCAGAAGGACTTTCTCTACTTCTTTGGATTAGGCCCTATGGTTGCCTTGTTCAAGTATTCCCCTGTTACGACTTATACTGTTTCTGTGCCTCCTCTGAAGCTTCCATTCAGCAGTTCAATTAGACAAGACTGGCTTATGAAAGAAACTCAGAATGTGTACGTGAAATTGATTTACTTGTTCACAGAGGTTGCCAACTCTTTAGAGAAGATGAGATCTCAATTTGATGGGTTGACTTTGAAACTTCGAGGCTCATTTAAAGAATTCTCTGACATCGAAGAGATGCTAAAGCAAGAAAAATCTGAGTTAGAGGTAGTGCTCAAGAATGCTGTTACCAGGACGGAGAACTCGGACCAGGTTGCCTACAAACTACTTAGCTTGAACAGAGTACTTTGGGAACTGCTCCTTGAAGCATGTATTTGGGAACGGCGCATGCAGTCATTACTGTCACCAGATCCTACGATGATCCATTCTAGAGAATCTGTGAAGGCAGAGCCAGAAAAAGTTGATTCATATATTGGTACCAGTTTAGAAGTCAAATTGGATACAGCTGCAGAAGCTGATGGATCTCCAATAAAAGACATTCCAGTTGAAGGTCCTGTTCAAGAATCTAATGGAGCTGATCCAGTTAACGTGTCTGATTCGGCTGAGGGTTTCAAAACACCGAATGTGGATGGTTCAAGCCCAAAACGGTTGGCCAGGCAGGGGTCCAATCTGAGGAATGGCTCCAGCTATGATCATTGTGAGACTGATCAGCTAGCAGAAAATGTGGATGGTGCTTTTGCCGATTCAGATCTATCAATGAAAGGCACTTATCACCGTTCACTATCATTCAACTTGGAAGGTTCACATGACTGGTTTTGGGCACCATTCTCCGAAATTCGTCAAGTTGGCATGAGAGATCTACAGAGAGTGTTCTTTCGGAAGTTTGAATCAATTAGTAGCTATACTGCAGAGAACTTACCCACAGCGCATCAGCTGATCACGGAAGAAGGGCAAATGCTGCACATTCCTCTTGGAACTGATAACCATGTTGTGTCAGACTATGAAGGAGAACTGTCAAGTATGATTGCTTGTGCACTGGCTTTATTGAAAGATCTACCTTTACAAACAGAGGTTCATGCTGACAATAGCAAGGGAGAGAGTGGAGTTGCTGCGAGAACATTTGAGAATTTGCACGGTCTGACTCGACTTCCCACTAATACTTCCCTGGATTGGTCTTCAAATGGTTCGTTACATTTAGATTCTGTCCATAATATGGCAAGCATTTTTTCAGATGAGTCACGCTTCTCCAGTTTTGATGGGTTGAATTTGTTGGATTCTCTGGTTCCGCCTGGAACTGTTAACCCAGTAGTCCCTCTAGGAGTTTCAAAATCATTTGGCAAGGATAAATATACTGTGATATGTCCATATGCCAATCAATTTCGTGATCTCCGCAATCGATGCTGCCAATCTGAAGTTGATTACATTGCTTCCCTTAGCCGTTGCAGAAACTGGGATGCTAAAGGTGGGAAGAGCAAATCCTTTTTTGCCAAAACACTTGATGACAGGTTGATTATTAAAGAAATCAAGAGGACAGAATTTGAGTCGTTTATGAAGTTTTCCGATGAGTATTTCAAGCACGTAAAGCTTTCATTTGACAATGGGAATCAAACATGCCTTGCGAAAGTTCTTGGGATTTATCAGGTGATTGTGAGACAGACAAAAAGTGGGAAAGAGATGAGGCATGATCTGATGGTCATGGAGAATCTTACCTTTGGTCGGAACATCACTCGCCAGTATGATCTTAAAGGTGCTCTACATGCTCGATTCAATTCTGCTAGTGGTGGTTCGGGAGATGTCCTTTTGGATCAGAACTTTGTCAATGACATGAACTCATCTCCACTGTATGTTAGTAACAAAGCAAAGCGTATATTGCAGCGGGCTGTGTGGAATGACACAACTTTCCTCAATTCAATCAATGTTATGGATTATTCATTGCTTGTGGGAGTAGATACTGAACGTCAGGAGCTTGTATGTGGCATTATTGATTATCTTAGGCAGTATACATGGGACAAGCAGCTAGAGACATGGGTGAAGTCTTCACTTGTGCCGAAAAATGTCTTGCCGACCGTCATCTCGCCAATAGAGTACAAGAGGAGATTCAGAAGGTTCATGTCTAAGCATTTCTTGAGCGTCCCCGATGATTGGTGCTCACCAGAATCCTCTGACCCGTCACCAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

1609

Amino Acids

178.95

Weight (kDa)

5.54

Isoelectric Point (pI)

43.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	263 - 512	2.2e-21	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1370 - 1535	2.3e-33	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1535 - 1588	3.9e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34260 AT1G34260 AT1G34260 AT1G34260
fragaria_vesca	FvH4_2g12440 FvH4_2g12440 FvH4_2g12440 FvH4_2g12440 FvH4_2g12440
malus_domestica	MD00G1078100.v1.1 MD10G1044000.v1.1 MD10G1044100.v1.1
prunus_persica	Prupe.8G050600_v2.0.a1
pyrus_communis	pycom05g03120 pycom05g03130 pycom10g03060 pycom10g03070 pycom10g03080
rosa_chinensis	RchiOBHm_Chr6g0272731
rosa_laevigata	RLG00000013661
rosa_multiflora	Rmu_co8449107.1_g000001 Rmu_sc0003080.1_g000001 Rmu_sc0014453.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196050
rosa_rugosa	Rorug06G0070400
rosa_samantha	Rh6AG186600 Rh6BG189900 Rh6CG188700 Rh6DG180100
rosa_wichuraiana	Rw6G016100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	218
AatII	GACGTC	1 cut(s)	1770
Acc65I	GGTACC	1 cut(s)	3122
AccB1I	GGYRCC	2 cut(s)	3122, 3466
AccB7I	CCANNNNNTGG	3 cut(s)	365, 1267, 3859
AccI	GTMKAC	3 cut(s)	434, 1028, 4646
AciI	CCGC	7 cut(s)	119, 294, 755, 2592, 3932, 4036, 4522
AcoI	YGGCCR	2 cut(s)	2593, 3297
AcuI	CTGAAG	3 cut(s)	1154, 2720, 4077
AcvI	CACGTG	1 cut(s)	2519
AcyI	GRCGYC	1 cut(s)	1767
AdeI	CACNNNGTG	2 cut(s)	518, 1478
AfiI	CCNNNNNNNGG	9 cut(s)	365, 1267, 3194, 3306, 3318, 3816, 3859, 4362, 4788
AflIII	ACRYGT	1 cut(s)	3234
AhlI	ACTAGT	3 cut(s)	506, 979, 1682
AjnI	CCWGG	9 cut(s)	1266, 1443, 2392, 2541, 2936, 2955, 3299, 3810, 3934
AleI	CACNNNNGTG	2 cut(s)	12, 4822
Alw21I	GWGCWC	8 cut(s)	257, 1474, 2309, 2550, 2922, 3682, 4399, 4796
Alw26I	GTCTC	8 cut(s)	226, 1541, 1673, 1899, 2393, 3341, 4291, 4661
Alw44I	GTGCAC	2 cut(s)	2305, 3678
AlwNI	CAGNNNCTG	2 cut(s)	3161, 4095
Ama87I	CYCGRG	1 cut(s)	1544
AoxI	GGCC	4 cut(s)	91, 2593, 2635, 3297
ApaLI	GTGCAC	2 cut(s)	2305, 3678
AseI	ATTAAT	2 cut(s)	762, 1415
Asp718I	GGTACC	1 cut(s)	3122
AspLEI	GCGC	2 cut(s)	3033, 3574
AspS9I	GGNCC	6 cut(s)	92, 1399, 2636, 2953, 3197, 3309
AsuC2I	CCSGG	1 cut(s)	2412
AsuII	TTCGAA	1 cut(s)	65
AsuNHI	GCTAGC	1 cut(s)	3358
AvaI	CYCGRG	1 cut(s)	1544
AvaII	GGWCC	4 cut(s)	1399, 2953, 3197, 3309
AxyI	CCTNAGG	1 cut(s)	1974
BaeGI	GKGCMC	3 cut(s)	2309, 3469, 3682
BalI	TGGCCA	1 cut(s)	3299
BamHI	GGATCC	1 cut(s)	919
BanI	GGYRCC	2 cut(s)	3122, 3466
BanII	GRGCYC	2 cut(s)	257, 2550
BauI	CACGAG	1 cut(s)	1200
BbrPI	CACGTG	1 cut(s)	2519
BbsI	GAAGAC	4 cut(s)	988, 1074, 3813, 4674
Bbv12I	GWGCWC	8 cut(s)	257, 1474, 2309, 2550, 2922, 3682, 4399, 4796
BbvCI	CCTCAGC	1 cut(s)	3249
BceAI	ACGGC	3 cut(s)	2202, 3044, 4068
BciT130I	CCWGG	9 cut(s)	1268, 1445, 2394, 2543, 2938, 2957, 3301, 3812, 3936
BciVI	GTATCC	1 cut(s)	3139
BclI	TGATCA	5 cut(s)	180, 783, 3337, 3352, 3582
BcnI	CCSGG	1 cut(s)	2412
BcoDI	GTCTC	8 cut(s)	226, 1541, 1673, 1899, 2393, 3341, 4291, 4661
BcuI	ACTAGT	3 cut(s)	506, 979, 1682
BfmI	CTRYAG	3 cut(s)	3153, 3506, 3552
BfuI	GTATCC	1 cut(s)	3139
BglII	AGATCT	5 cut(s)	1949, 2816, 3394, 3502, 3697
BmcAI	AGTACT	2 cut(s)	1071, 2991
Bme18I	GGWCC	4 cut(s)	1399, 2953, 3197, 3309
BmeT110I	CYCGRG	1 cut(s)	1544
BmgT120I	GGNCC	6 cut(s)	92, 1399, 2636, 2953, 3197, 3309
BmiI	GGNNCC	9 cut(s)	94, 576, 921, 1401, 3124, 3310, 3328, 3468, 3930
BmrI	ACTGGG	3 cut(s)	1704, 3944, 4105
BmsI	GCATC	8 cut(s)	824, 886, 1847, 1886, 2874, 3583, 4034, 4090
BmtI	GCTAGC	1 cut(s)	3362
BmuI	ACTGGG	3 cut(s)	1704, 3944, 4105
BpiI	GAAGAC	4 cut(s)	988, 1074, 3813, 4674
BplI	GAGNNNNNCTC	2 cut(s)	1264, 1296
BpmI	CTGGAG	9 cut(s)	77, 250, 717, 1289, 1404, 2415, 2564, 3313, 3877
Bpu10I	CCTNAGC	3 cut(s)	404, 3249, 4079
Bpu14I	TTCGAA	1 cut(s)	65
BpuEI	CTTGAG	5 cut(s)	285, 328, 2099, 2906, 4792
BpuMI	CCSGG	1 cut(s)	2412
Bsa29I	ATCGAT	1 cut(s)	4042
BsaAI	YACGTR	4 cut(s)	934, 2519, 2764, 4225
BsaBI	GATNNNNATC	2 cut(s)	1096, 3393
BsaHI	GRCGYC	1 cut(s)	1767
BsaJI	CCNNGG	7 cut(s)	36, 804, 1046, 1444, 1761, 2392, 3810
BsaXI	ACNNNNNCTCC	4 cut(s)	1383, 1413, 2434, 2464
Bsc4I	CCNNNNNNNGG	9 cut(s)	365, 1267, 3194, 3306, 3318, 3816, 3859, 4362, 4788
Bse21I	CCTNAGG	1 cut(s)	1974
Bse3DI	GCAATG	7 cut(s)	753, 921, 948, 1581, 1614, 4068, 4577
Bse8I	GATNNNNATC	2 cut(s)	1096, 3393
BseBI	CCWGG	9 cut(s)	1268, 1445, 2394, 2543, 2938, 2957, 3301, 3812, 3936
BseCI	ATCGAT	1 cut(s)	4042
BseDI	CCNNGG	7 cut(s)	36, 804, 1046, 1444, 1761, 2392, 3810
BseGI	GGATG	9 cut(s)	355, 607, 1097, 1165, 1748, 2442, 3280, 3379, 4105
BseJI	GATNNNNATC	2 cut(s)	1096, 3393
BseLI	CCNNNNNNNGG	9 cut(s)	365, 1267, 3194, 3306, 3318, 3816, 3859, 4362, 4788
BseMI	GCAATG	7 cut(s)	753, 921, 948, 1581, 1614, 4068, 4577
BseRI	GAGGAG	3 cut(s)	1388, 2685, 4753
BseSI	GKGCMC	3 cut(s)	2309, 3469, 3682
BsgI	GTGCAG	6 cut(s)	39, 996, 1529, 1536, 2499, 3590
Bsh1285I	CGRYCG	1 cut(s)	4713
BshFI	GGCC	4 cut(s)	93, 2595, 2637, 3299
BshNI	GGYRCC	2 cut(s)	3122, 3466
BshVI	ATCGAT	1 cut(s)	4042
BsiEI	CGRYCG	1 cut(s)	4713
BsiHKAI	GWGCWC	8 cut(s)	257, 1474, 2309, 2550, 2922, 3682, 4399, 4796
BsiHKCI	CYCGRG	1 cut(s)	1544
BsiSI	CCGG	1 cut(s)	2412
BslFI	GGGAC	4 cut(s)	1385, 3941, 4667, 4763
BslI	CCNNNNNNNGG	9 cut(s)	365, 1267, 3194, 3306, 3318, 3816, 3859, 4362, 4788
BsmAI	GTCTC	8 cut(s)	226, 1541, 1673, 1899, 2393, 3341, 4291, 4661
BsmBI	CGTCTC	1 cut(s)	2393
BsmFI	GGGAC	4 cut(s)	1385, 3941, 4667, 4763
BsmI	GAATGC	2 cut(s)	2191, 2932
BsnI	GGCC	4 cut(s)	93, 2595, 2637, 3299
BsoBI	CYCGRG	1 cut(s)	1544
Bsp119I	TTCGAA	1 cut(s)	65
Bsp1286I	GDGCHC	9 cut(s)	257, 1474, 2309, 2550, 2922, 3469, 3682, 4399, 4796
Bsp1407I	TGTACA	2 cut(s)	1964, 2486
BspACI	CCGC	7 cut(s)	119, 294, 755, 2592, 3932, 4036, 4522
BspANI	GGCC	4 cut(s)	93, 2595, 2637, 3299
BspDI	ATCGAT	1 cut(s)	4042
BspHI	TCATGA	1 cut(s)	1735
BspLI	GGNNCC	9 cut(s)	94, 576, 921, 1401, 3124, 3310, 3328, 3468, 3930
BspMAI	CTGCAG	2 cut(s)	3157, 3556
BspOI	GCTAGC	1 cut(s)	3362
BspQI	GCTCTTC	1 cut(s)	4109
BspT104I	TTCGAA	1 cut(s)	65
BspT107I	GGYRCC	2 cut(s)	3122, 3466
BsrDI	GCAATG	7 cut(s)	753, 921, 948, 1581, 1614, 4068, 4577
BsrGI	TGTACA	2 cut(s)	1964, 2486
BssECI	CCNNGG	7 cut(s)	36, 804, 1046, 1444, 1761, 2392, 3810
BssNAI	GTATAC	2 cut(s)	435, 4647
BssNI	GRCGYC	1 cut(s)	1767
BssSI	CACGAG	1 cut(s)	1200
BssT1I	CCWWGG	3 cut(s)	36, 1046, 1761
Bst1107I	GTATAC	2 cut(s)	435, 4647
Bst2BI	CACGAG	1 cut(s)	1200
Bst2UI	CCWGG	9 cut(s)	1268, 1445, 2394, 2543, 2938, 2957, 3301, 3812, 3936
Bst6I	CTCTTC	4 cut(s)	1918, 2016, 2874, 4109
BstACI	GRCGYC	1 cut(s)	1767
BstAPI	GCANNNNNTGC	2 cut(s)	3604, 4044
BstAUI	TGTACA	2 cut(s)	1964, 2486
BstBAI	YACGTR	4 cut(s)	934, 2519, 2764, 4225
BstBI	TTCGAA	1 cut(s)	65
BstC8I	GCNNGC	7 cut(s)	502, 1008, 1380, 3035, 3360, 3865, 4524
BstDSI	CCRYGG	1 cut(s)	804
BstF5I	GGATG	9 cut(s)	355, 607, 1097, 1165, 1748, 2442, 3280, 3379, 4105
BstHHI	GCGC	2 cut(s)	3033, 3574
BstMAI	GTCTC	8 cut(s)	226, 1541, 1673, 1899, 2393, 3341, 4291, 4661
BstMCI	CGRYCG	1 cut(s)	4713
BstMWI	GCNNNNNNNGC	9 cut(s)	752, 1604, 2293, 3158, 3604, 4044, 4228, 4403, 4516
BstNI	CCWGG	9 cut(s)	1268, 1445, 2394, 2543, 2938, 2957, 3301, 3812, 3936
BstNSI	RCATGY	6 cut(s)	1010, 1382, 3018, 3037, 4260, 4406
BstSFI	CTRYAG	3 cut(s)	3153, 3506, 3552
BstSLI	GKGCMC	3 cut(s)	2309, 3469, 3682
BstV2I	GAAGAC	4 cut(s)	988, 1074, 3813, 4674
BstX2I	RGATCY	9 cut(s)	307, 919, 1949, 2816, 3055, 3167, 3394, 3502, 3697
BstYI	RGATCY	9 cut(s)	307, 919, 1949, 2816, 3055, 3167, 3394, 3502, 3697
BstZ17I	GTATAC	2 cut(s)	435, 4647
Bsu15I	ATCGAT	1 cut(s)	4042
Bsu36I	CCTNAGG	1 cut(s)	1974
BsuI	GTATCC	1 cut(s)	3139
BsuRI	GGCC	4 cut(s)	93, 2595, 2637, 3299
BsuTUI	ATCGAT	1 cut(s)	4042
BtgI	CCRYGG	1 cut(s)	804
BtgZI	GCGATG	1 cut(s)	1247
BtsCI	GGATG	9 cut(s)	355, 607, 1097, 1165, 1748, 2442, 3280, 3379, 4105
BtsI	GCAGTG	3 cut(s)	214, 430, 1517
Cac8I	GCNNGC	7 cut(s)	502, 1008, 1380, 3035, 3360, 3865, 4524
CaiI	CAGNNNCTG	2 cut(s)	3161, 4095
CciI	TCATGA	1 cut(s)	1735
CfoI	GCGC	2 cut(s)	3033, 3574
Cfr13I	GGNCC	6 cut(s)	92, 1399, 2636, 2953, 3197, 3309
ClaI	ATCGAT	1 cut(s)	4042
CseI	GACGC	1 cut(s)	4765
CsiI	ACCWGGT	1 cut(s)	2955
DraI	TTTAAA	4 cut(s)	727, 775, 2059, 2860
DraIII	CACNNNGTG	2 cut(s)	518, 1478
DrdI	GACNNNNNNGTC	1 cut(s)	218
DseDI	GACNNNNNNGTC	1 cut(s)	218
EaeI	YGGCCR	2 cut(s)	2593, 3297
Eam1104I	CTCTTC	4 cut(s)	1918, 2016, 2874, 4109
EarI	CTCTTC	4 cut(s)	1918, 2016, 2874, 4109
EciI	GGCGGA	1 cut(s)	3921
Ecl136II	GAGCTC	2 cut(s)	255, 2548
Eco130I	CCWWGG	3 cut(s)	36, 1046, 1761
Eco24I	GRGCYC	2 cut(s)	257, 2550
Eco47I	GGWCC	4 cut(s)	1399, 2953, 3197, 3309
Eco53kI	GAGCTC	2 cut(s)	255, 2548
Eco57I	CTGAAG	3 cut(s)	1154, 2720, 4077
Eco72I	CACGTG	1 cut(s)	2519
Eco81I	CCTNAGG	1 cut(s)	1974
Eco88I	CYCGRG	1 cut(s)	1544
EcoICRI	GAGCTC	2 cut(s)	255, 2548
EcoO109I	RGGNCCY	4 cut(s)	92, 1399, 2636, 3197
EcoRI	GAATTC	2 cut(s)	61, 2864
EcoRII	CCWGG	9 cut(s)	1266, 1443, 2392, 2541, 2936, 2955, 3299, 3810, 3934
EcoT14I	CCWWGG	3 cut(s)	36, 1046, 1761
EcoT22I	ATGCAT	2 cut(s)	950, 2191
EcoT38I	GRGCYC	2 cut(s)	257, 2550
ErhI	CCWWGG	3 cut(s)	36, 1046, 1761
Esp3I	CGTCTC	1 cut(s)	2393
FaqI	GGGAC	4 cut(s)	1385, 3941, 4667, 4763
FauI	CCCGC	1 cut(s)	4515
FauNDI	CATATG	2 cut(s)	1023, 4012
FbaI	TGATCA	5 cut(s)	180, 783, 3337, 3352, 3582
FblI	GTMKAC	3 cut(s)	434, 1028, 4646
FokI	GGATG	9 cut(s)	342, 614, 1084, 1152, 1755, 2449, 3287, 3386, 4112
FriOI	GRGCYC	2 cut(s)	257, 2550
GlaI	GCGC	2 cut(s)	3032, 3573
GsuI	CTGGAG	9 cut(s)	77, 250, 717, 1289, 1404, 2415, 2564, 3313, 3877
HaeIII	GGCC	4 cut(s)	93, 2595, 2637, 3299
HapII	CCGG	1 cut(s)	2412
HgaI	GACGC	1 cut(s)	4765
HhaI	GCGC	2 cut(s)	3033, 3574
Hin1I	GRCGYC	1 cut(s)	1767
Hin6I	GCGC	2 cut(s)	3031, 3572
HinP1I	GCGC	2 cut(s)	3031, 3572
HincII	GTYRAC	6 cut(s)	977, 1029, 1564, 2835, 3232, 3946
HindII	GTYRAC	6 cut(s)	977, 1029, 1564, 2835, 3232, 3946
HindIII	AAGCTT	3 cut(s)	2070, 2702, 4229
HpaI	GTTAAC	2 cut(s)	3232, 3946
HpaII	CCGG	1 cut(s)	2412
Hpy99I	CGWCG	1 cut(s)	1772
HpyCH4IV	ACGT	9 cut(s)	722, 933, 1767, 2386, 2518, 2763, 3234, 4224, 4603
HpyF10VI	GCNNNNNNNGC	9 cut(s)	752, 1604, 2293, 3158, 3604, 4044, 4228, 4403, 4516
HpySE526I	ACGT	9 cut(s)	722, 933, 1767, 2386, 2518, 2763, 3234, 4224, 4603
Hsp92I	GRCGYC	1 cut(s)	1767
HspAI	GCGC	2 cut(s)	3031, 3572
KpnI	GGTACC	1 cut(s)	3126
Ksp22I	TGATCA	5 cut(s)	180, 783, 3337, 3352, 3582
KspAI	GTTAAC	2 cut(s)	3232, 3946
LguI	GCTCTTC	1 cut(s)	4109
LweI	GCATC	8 cut(s)	824, 886, 1847, 1886, 2874, 3583, 4034, 4090
MabI	ACCWGGT	1 cut(s)	2955
MaeII	ACGT	9 cut(s)	722, 933, 1767, 2386, 2518, 2763, 3234, 4224, 4603
MflI	RGATCY	9 cut(s)	307, 919, 1949, 2816, 3055, 3167, 3394, 3502, 3697
MhlI	GDGCHC	9 cut(s)	257, 1474, 2309, 2550, 2922, 3469, 3682, 4399, 4796
MlsI	TGGCCA	1 cut(s)	3299
MluNI	TGGCCA	1 cut(s)	3299
MlyI	GAGTC	6 cut(s)	697, 1340, 2452, 3781, 3890, 4196
MmeI	TCCRAC	3 cut(s)	2127, 3897, 4343
Mox20I	TGGCCA	1 cut(s)	3299
Mph1103I	ATGCAT	2 cut(s)	950, 2191
MscI	TGGCCA	1 cut(s)	3299
MslI	CAYNNNNRTG	5 cut(s)	12, 519, 1170, 1992, 4822
Msp20I	TGGCCA	1 cut(s)	3299
MspA1I	CMGCKG	3 cut(s)	3152, 3580, 4522
MspI	CCGG	1 cut(s)	2412
Mva1269I	GAATGC	2 cut(s)	2191, 2932
MvaI	CCWGG	9 cut(s)	1268, 1445, 2394, 2543, 2938, 2957, 3301, 3812, 3936
MwoI	GCNNNNNNNGC	9 cut(s)	752, 1604, 2293, 3158, 3604, 4044, 4228, 4403, 4516
NciI	CCSGG	1 cut(s)	2412
NdeI	CATATG	2 cut(s)	1023, 4012
NheI	GCTAGC	1 cut(s)	3358
NlaIV	GGNNCC	9 cut(s)	94, 576, 921, 1401, 3124, 3310, 3328, 3468, 3930
NmuCI	GTSAC	5 cut(s)	5, 209, 3048, 3882, 4815
NsiI	ATGCAT	2 cut(s)	950, 2191
NspI	RCATGY	6 cut(s)	1010, 1382, 3018, 3037, 4260, 4406
NspV	TTCGAA	1 cut(s)	65
OliI	CACNNNNGTG	2 cut(s)	12, 4822
PaeI	GCATGC	3 cut(s)	1010, 1382, 3037
PaeR7I	CTCGAG	1 cut(s)	1544
PagI	TCATGA	1 cut(s)	1735
PciSI	GCTCTTC	1 cut(s)	4109
PcsI	WCGNNNNNNNCGW	1 cut(s)	2383
PctI	GAATGC	2 cut(s)	2191, 2932
PflFI	GACNNNGTC	1 cut(s)	31
PflMI	CCANNNNNTGG	3 cut(s)	365, 1267, 3859
PfoI	TCCNGGA	1 cut(s)	2541
PleI	GAGTC	6 cut(s)	696, 1340, 2451, 3781, 3889, 4195
PmaCI	CACGTG	1 cut(s)	2519
PmlI	CACGTG	1 cut(s)	2519
PpsI	GAGTC	6 cut(s)	696, 1340, 2451, 3781, 3889, 4195
Ppu21I	YACGTR	4 cut(s)	934, 2519, 2764, 4225
PpuMI	RGGWCCY	2 cut(s)	1399, 3197
PshBI	ATTAAT	2 cut(s)	762, 1415
Psp124BI	GAGCTC	2 cut(s)	257, 2550
Psp5II	RGGWCCY	2 cut(s)	1399, 3197
Psp6I	CCWGG	9 cut(s)	1266, 1443, 2392, 2541, 2936, 2955, 3299, 3810, 3934
PspCI	CACGTG	1 cut(s)	2519
PspGI	CCWGG	9 cut(s)	1266, 1443, 2392, 2541, 2936, 2955, 3299, 3810, 3934
PspN4I	GGNNCC	9 cut(s)	94, 576, 921, 1401, 3124, 3310, 3328, 3468, 3930
PspPI	GGNCC	6 cut(s)	92, 1399, 2636, 2953, 3197, 3309
PspPPI	RGGWCCY	2 cut(s)	1399, 3197
PsrI	GAACNNNNNNTAC	2 cut(s)	664, 696
PstI	CTGCAG	2 cut(s)	3157, 3556
PstNI	CAGNNNCTG	2 cut(s)	3161, 4095
PsuI	RGATCY	9 cut(s)	307, 919, 1949, 2816, 3055, 3167, 3394, 3502, 3697
PsyI	GACNNNGTC	1 cut(s)	31
PvuII	CAGCTG	2 cut(s)	3152, 3580
RseI	CAYNNNNRTG	5 cut(s)	12, 519, 1170, 1992, 4822
SacI	GAGCTC	2 cut(s)	257, 2550
SalI	GTCGAC	1 cut(s)	1027
SapI	GCTCTTC	1 cut(s)	4109
Sau96I	GGNCC	6 cut(s)	92, 1399, 2636, 2953, 3197, 3309
ScaI	AGTACT	2 cut(s)	1071, 2991
SchI	GAGTC	6 cut(s)	697, 1340, 2452, 3781, 3890, 4196
SduI	GDGCHC	9 cut(s)	257, 1474, 2309, 2550, 2922, 3469, 3682, 4399, 4796
SexAI	ACCWGGT	1 cut(s)	2955
SfaNI	GCATC	8 cut(s)	824, 886, 1847, 1886, 2874, 3583, 4034, 4090
SfcI	CTRYAG	3 cut(s)	3153, 3506, 3552
Sfr274I	CTCGAG	1 cut(s)	1544
SfuI	TTCGAA	1 cut(s)	65
SinI	GGWCC	4 cut(s)	1399, 2953, 3197, 3309
SlaI	CTCGAG	1 cut(s)	1544
SmiI	ATTTAAAT	1 cut(s)	2059
SmiMI	CAYNNNNRTG	5 cut(s)	12, 519, 1170, 1992, 4822
SmlI	CTYRAG	6 cut(s)	300, 343, 1544, 2078, 2921, 4771
SmoI	CTYRAG	6 cut(s)	300, 343, 1544, 2078, 2921, 4771
SpeI	ACTAGT	3 cut(s)	506, 979, 1682
SphI	GCATGC	3 cut(s)	1010, 1382, 3037
SsiI	CCGC	7 cut(s)	119, 294, 755, 2592, 3932, 4036, 4522
SstI	GAGCTC	2 cut(s)	257, 2550
StyI	CCWWGG	3 cut(s)	36, 1046, 1761
SwaI	ATTTAAAT	1 cut(s)	2059
TaiI	ACGT	9 cut(s)	725, 936, 1770, 2389, 2521, 2766, 3237, 4227, 4606
TaqII	GACCGA	1 cut(s)	309
TatI	WGTACW	8 cut(s)	1035, 1069, 1286, 1810, 1964, 2486, 2989, 4731
TauI	GCSGC	1 cut(s)	2595
TseFI	GTSAC	5 cut(s)	5, 209, 3048, 3882, 4815
Tsp45I	GTSAC	5 cut(s)	5, 209, 3048, 3882, 4815
TspGWI	ACGGA	5 cut(s)	793, 1489, 1948, 2957, 3602
Tth111I	GACNNNGTC	1 cut(s)	31
Van91I	CCANNNNNTGG	3 cut(s)	365, 1267, 3859
VneI	GTGCAC	2 cut(s)	2305, 3678
VpaK11BI	GGWCC	4 cut(s)	1399, 2953, 3197, 3309
VspI	ATTAAT	2 cut(s)	762, 1415
XbaI	TCTAGA	1 cut(s)	3074
XceI	RCATGY	6 cut(s)	1010, 1382, 3018, 3037, 4260, 4406
XhoI	CTCGAG	1 cut(s)	1544
XmiI	GTMKAC	3 cut(s)	434, 1028, 4646
ZraI	GACGTC	1 cut(s)	1768
ZrmI	AGTACT	2 cut(s)	1071, 2991
Zsp2I	ATGCAT	2 cut(s)	950, 2191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.