Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000814

Physical Location & Seq

Genomic Coordinates Forward (+)

35662874 .. 35668505

Gene Structure 5632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1160300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2895 bp

ATGGTGGCCAGAGACATATCGAATTCGTGCTCCTTGAGTGCCAAGTTAGAGGAATTCCCCGGGTTTCCGCCATCAATGGCCGATCATTCACGCGGCAGCGGCCGCAGCCGTGCGAGCTTCTGGGCTCAGTCCAACGCTTTGCTCCGAAAGAACTTAACTTTTCAGAAACGAAATATGTGCTCGAATATATCGTTGGTGTTGATACCTGGATTGCTCTGCTTGATTTTGCTTGGCATCCAAATTGCGTTGAACAAAGCAAAAGCCAGCGGTGGCGGTGACAAGTGTGACGGCAGTCATACCGATACAGACTGGAGTGGTTTAGGCCCTTGTCCAATTCCTAACCCACCAGAATGGGCTCCGCTGTTATACATCTCCAATGCCGCTTTTCGTGCCGTGAGAAGCGACTCGATTCTTCCCTTCACAGACTTGCCTGACGCGTCATGCCGGAACAGCAAGAACAACGTCACCTGCCCTCTCACTGTGCTTGTCACCGGCGACAACCAGTCTGTCGGACAAATTTTGGCTGGAAATATGTTTCCAAATGATCCTTTCCCTCTGAATTCCTCTGATGTTATGGATTATCTAGTCAATAATGTCATGGGAACGTATGATCTCCCTAGCGCACCCAACTTTTATGATCCTGCATTCGATGATCCACTTTTTAGAGTGCAGCGGCAGTGCTCCCCAAACTCTACAATCTATATTCCACCATATGGATCGACATCATCCGAGTCTAACCTAGAGGTTAGATGCGTTGAAGCTTTGCAATTATGGCGTAAATCTTCATCTGAGATAAACAATGAGCTATACGAAGGCTACCCAAAGGGCAATCCAGAGAGGAAGGTTAACGAAATACTTGCGGCCTACGATTTCTCCAACTCCGATGACAACAAATTTGACGTAACCGTATGGTACAATTCAAGCTTAAACGATGGCTACCTGGATGACGACCAGAGCAGCACGCTGCGGGTTGGGCGCTCCCTGAATCTGGCATCCAATGCCTACCTTCAGTTTTTAAAAGGATCAAGCACCAAAATGTTGTTCGAGTTTGTCAAAGAATTTCCCAAGCCTTTATCTGCCAGGATGTCGATGCTAGATATTCCCTCTATGATTGGTCCACTCTTCTATACTTGGATCATTTTATTGCTCTTCCCTGTTGTGTTGAAGTATCTGGTTTATGAGAAACAACAAAACCTGAGAATCATGATGAAAATGCATGGACTTGGTGATGCACCATATTGGATGATTTCCTATGCTTACTTTCTTACAGTTTCTTCAATCTACATGCTATTTTTTATTGCAGTTGGCTCCATCATAGGGTTAAAGTTCTTCACAATCAATGACTACAGCATCCAGTTTGTTTTCTACTTCATCTATCTGAACTTGCAAATTGCAGTGGCTTTTCTTGTAGCTTCGATGTTTTCCAATGTTAAGGCTTGTACAGTTATTGGTTACATTTTGGTCTTTGGAACTGGGCTTGTGGCAGACAATCTATTTCGAAGCATTATCGAAGATGAATCTGTCTCTAAGCGTGTGATCATCCTTATAGAGCTGTATCCTGGCTTCTCTCTGTATCGTGGACTCTACGAGTTTGCACAGTACCCCATCCAATATAAAAAAGTAGGGATGCGATGGGGCGATTTGAGTGACGGGAGTAAAGGGATGAGAGAGGTCTTGATTATCATGGTTATCGAGTGGTTTGTGGTGCTTCTTGTTGCATACTGCATTGACCAACTTGTATCATCTGGAAAAAGTCCTTTTGTTTTCTTGCAAAGGTTTAGGAAGAAGAAACTTCCATCTATTCGGAGGCCTAGTATGCAAGGGCAGGGATCTAGTTCGATTCAGATGGAGAAACCGGATGTTTGTCATGAGATCGAGATGGTTGAGAGGTTGGTAGTGGAACGGGATACAAGTCACGCAATCGTATGTGACAACCTCCAAAAGGTGTACCCAGGAAGAGATGGAAACCCTGATAAATTTGCAGTGAAAGGGTGTTCTCTTGCCTTACCTAGAGGTGAATGCTTTGGCATGCTTGGCCCTAATGGTGCGGGGAAGACCTCTTTTATTAGTATGATGATTGGTCTCACCAAACCAACCTCTGGCACGGGGTATGTTCATGGTATGGACATTCGGACGCAGATGAACAAAATATATAGCAGCATGGGATTTTGTCCACAGCACGACCTTCTGTGGGAAACACTGACTGGAAGGGAGCATCTCCTATTTTATGGCAGACTTAAGAACCTAAAAGGTTCTGCCTTAATGCAAGCGGTGGAAGAATCTTTGAAGAGTGTCAATCTATTTCACGGAGGGGTTCCTGACAAACAAGCCGGGAAGTATAGTGGTGGTATGAAGCGAAGGCTTAGCGTTGCAATTTCGCTGATAGGTGACCCAAAAGTTGTCTACATGGATGAGCCTAGTACTGGACTTGATCCGGCTTCAAGAAACCATCTGTGGAACGTTATAAAGTCTGCGAAACAAGATCGTGCAATCATCCTAACCACACATTCCATGGAAGAGGCAGAGGTTCTATGTGATCGATTGGGGATTTTCGTCGATGGCAGGATGCAGTGCATAGGAAATCCAAAGGAGCTGAAGGCCAGATATGGAGGATCATATATCTTTACGATGACAACGAGTTGGCATCATGAGGAAGAAGTGGAGAGGATGGTGAGGAACATGTCACCGAACGCCGTCAAGACATACCGGCTCTCAGGGACGCAGAAGTTTGAGCTGCCCAAAACTGAGGTTAGAATTGCAGATGTGTTCCGCACGGTCGAGTGTGCGAAGTGTCGGTTCGCGGTGTTTGCATGGGGTGTGGCTGACACTACTCTGGAGGATGTGTTCATCAAGGTTGCTACAACTGCAGGATCATCGTCCGCTACACTTTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0006869 GO:0008150 GO:0009506 GO:0010876 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0030054 GO:0033036 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071702 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane_3 ABC_tran

Protein Analysis

965

Amino Acids

107.64

Weight (kDa)

8.2

Isoelectric Point (pI)

41.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC2_membrane_3	PF12698	283 - 574	3.6e-10	ABC-2 family transporter protein
ABC_tran	PF00005	663 - 807	6.7e-24	ABC transporter
AAA_21	PF13304	779 - 839	2.4e-08	AAA domain, putative AbiEii toxin, Type IV TA system
ABCA12_C	PF24526	873 - 923	1.4e-27	ABCA12-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47740 AT3G47750 AT3G47760 AT3G47760 AT3G47760 AT3G47770 AT3G47770 AT3G47770 AT3G47780 AT3G47790 AT3G47790 AT3G47790 AT5G61700 AT5G61700 AT5G61700 AT5G61700 AT5G61740 AT5G61740 AT5G61740
fragaria_vesca	FvH4_5g15270 FvH4_5g15270 FvH4_5g15270 FvH4_5g15270 FvH4_5g17020
malus_domestica	MD00G1160300.v1.1 MD06G1189700.v1.1 MD14G1196600.v1.1
prunus_persica	Prupe.5G188500_v2.0.a1 Prupe.5G188500_v2.0.a1 Prupe.5G188700_v2.0.a1 Prupe.5G229600_v2.0.a1 Prupe.5G229600_v2.0.a1
pyrus_communis	pycom06g16850 pycom14g16330
rosa_chinensis	RchiOBHm_Chr7g0178831 RchiOBHm_Chr7g0180791 RchiOBHm_Chr7g0180811
rosa_laevigata	RLG00000005228 RLG00000005229 RLG00000005411
rosa_multiflora	Rmu_sc0007457.1_g000007 Rmu_sc0007457.1_g000009 Rmu_sc0025325.1_g000002 Rmu_sc0025325.1_g000004
rosa_roxburghii	Rroxscaffold_3G00272510 Rroxscaffold_3G00272520 Rroxscaffold_3G00274540
rosa_rugosa	Rorug01G0115100 Rorug06G0420100 Rorug06G0420100 Rorug06G0436000 Rorug06G0436100 Rorug06G0436200 Rorug06G0436300
rosa_samantha	Rh1BG107000 Rh7AG019400 Rh7AG037800 Rh7AG038000 Rh7BG018900 Rh7BG037600 Rh7BG037800 Rh7CG019500 Rh7CG039200 Rh7CG039300 Rh7DG019300
rosa_wichuraiana	Rw7G001580 Rw7G003120 Rw7G003130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2465
AarI	CACCTGC	1 cut(s)	476
Acc36I	ACCTGC	1 cut(s)	476
AccB7I	CCANNNNNTGG	2 cut(s)	713, 2099
AccI	GTMKAC	1 cut(s)	2403
AccII	CGCG	3 cut(s)	93, 437, 2801
AclI	AACGTT	1 cut(s)	2460
AcoI	YGGCCR	3 cut(s)	6, 78, 100
AcsI	RAATTY	7 cut(s)	22, 53, 516, 559, 893, 1058, 1976
AcuI	CTGAAG	2 cut(s)	992, 2615
AfaI	GTAC	5 cut(s)	914, 1441, 1601, 1949, 2422
AfiI	CCNNNNNNNGG	7 cut(s)	713, 988, 1317, 1942, 2099, 2191, 2423
AflII	CTTAAG	1 cut(s)	2237
AflIII	ACRYGT	2 cut(s)	435, 2679
AgsI	TTSAA	7 cut(s)	250, 758, 921, 1165, 1278, 2287, 2442
AhdI	GACNNNNNGTC	1 cut(s)	502
AjnI	CCWGG	5 cut(s)	205, 939, 1079, 1558, 1951
AjuI	GAANNNNNNNTTGG	4 cut(s)	176, 208, 1025, 1057
AloI	GAACNNNNNNTCC	2 cut(s)	2828, 2860
AluBI	AGCT	8 cut(s)	117, 761, 805, 924, 1412, 1552, 2593, 2734
AluI	AGCT	8 cut(s)	117, 761, 805, 924, 1412, 1552, 2593, 2734
Alw21I	GWGCWC	3 cut(s)	32, 182, 683
Alw26I	GTCTC	3 cut(s)	6, 1528, 2087
Ama87I	CYCGRG	1 cut(s)	59
AoxI	GGCC	8 cut(s)	6, 78, 100, 322, 861, 1808, 2035, 2598
ApeKI	GCWGC	7 cut(s)	96, 105, 670, 957, 964, 2157, 2734
ApoI	RAATTY	7 cut(s)	22, 53, 516, 559, 893, 1058, 1976
ArsI	GACNNNNNNTTYG	2 cut(s)	878, 910
AspLEI	GCGC	2 cut(s)	623, 978
AspS9I	GGNCC	3 cut(s)	323, 1115, 2036
AsuC2I	CCSGG	3 cut(s)	60, 61, 2332
AsuHPI	GGTGA	9 cut(s)	287, 457, 481, 1238, 2027, 2077, 2399, 2676, 2683
AsuII	TTCGAA	1 cut(s)	1498
AvaI	CYCGRG	1 cut(s)	59
AvaII	GGWCC	1 cut(s)	1115
BalI	TGGCCA	1 cut(s)	8
BanII	GRGCYC	2 cut(s)	127, 358
BarI	GAAGNNNNNNTAC	2 cut(s)	1547, 1579
BbsI	GAAGAC	1 cut(s)	2060
Bbv12I	GWGCWC	3 cut(s)	32, 182, 683
BbvI	GCAGC	7 cut(s)	108, 117, 682, 951, 969, 2169, 2721
BceAI	ACGGC	4 cut(s)	93, 304, 377, 2678
BcgI	CGANNNNNNTGC	6 cut(s)	159, 193, 839, 873, 2856, 2890
BciT130I	CCWGG	5 cut(s)	207, 941, 1081, 1560, 1953
BciVI	GTATCC	2 cut(s)	1566, 1900
BclI	TGATCA	1 cut(s)	1536
BcnI	CCSGG	3 cut(s)	60, 61, 2332
BcoDI	GTCTC	3 cut(s)	6, 1528, 2087
BfaI	CTAG	8 cut(s)	584, 618, 740, 1094, 1812, 1833, 2010, 2418
BfmI	CTRYAG	2 cut(s)	1345, 2865
BfoI	RGCGCY	1 cut(s)	979
BfrI	CTTAAG	1 cut(s)	2237
BfuAI	ACCTGC	1 cut(s)	476
BfuI	GTATCC	2 cut(s)	1566, 1900
BlpI	GCTNAGC	1 cut(s)	2363
BmcAI	AGTACT	1 cut(s)	2422
Bme1390I	CCNGG	8 cut(s)	60, 61, 207, 941, 1081, 1560, 1953, 2332
Bme18I	GGWCC	1 cut(s)	1115
BmeRI	GACNNNNNGTC	1 cut(s)	502
BmeT110I	CYCGRG	1 cut(s)	59
BmgT120I	GGNCC	3 cut(s)	323, 1115, 2036
BmiI	GGNNCC	3 cut(s)	357, 1309, 2316
BmrFI	CCNGG	8 cut(s)	60, 61, 207, 941, 1081, 1560, 1953, 2332
BmrI	ACTGGG	1 cut(s)	1482
BmuI	ACTGGG	1 cut(s)	1482
BoxI	GACNNNNGTC	1 cut(s)	291
BpiI	GAAGAC	1 cut(s)	2060
BpmI	CTGGAG	2 cut(s)	331, 2855
Bpu1102I	GCTNAGC	1 cut(s)	2363
Bpu14I	TTCGAA	1 cut(s)	1498
BpuEI	CTTGAG	1 cut(s)	55
BpuMI	CCSGG	3 cut(s)	60, 61, 2332
Bsa29I	ATCGAT	1 cut(s)	2539
BsaBI	GATNNNNATC	1 cut(s)	721
BsaI	GGTCTC	1 cut(s)	2087
BsaJI	CCNNGG	4 cut(s)	58, 59, 1951, 2511
BsaWI	WCCGGW	1 cut(s)	1855
BsaXI	ACNNNNNCTCC	6 cut(s)	857, 887, 1798, 1828, 2828, 2858
Bsc4I	CCNNNNNNNGG	7 cut(s)	713, 988, 1317, 1942, 2099, 2191, 2423
Bse118I	RCCGGY	2 cut(s)	491, 2706
Bse1I	ACTGG	6 cut(s)	314, 502, 1354, 1477, 2209, 2428
Bse8I	GATNNNNATC	1 cut(s)	721
BseBI	CCWGG	5 cut(s)	207, 941, 1081, 1560, 1953
BseCI	ATCGAT	1 cut(s)	2539
BseDI	CCNNGG	4 cut(s)	58, 59, 1951, 2511
BseJI	GATNNNNATC	1 cut(s)	721
BseLI	CCNNNNNNNGG	7 cut(s)	713, 988, 1317, 1942, 2099, 2191, 2423
BseMII	CTCAG	5 cut(s)	140, 780, 1187, 2727, 2736
BseNI	ACTGG	6 cut(s)	314, 502, 1354, 1477, 2209, 2428
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	7 cut(s)	108, 117, 682, 951, 969, 2169, 2721
BsgI	GTGCAG	1 cut(s)	689
Bsh1236I	CGCG	3 cut(s)	93, 437, 2801
Bsh1285I	CGRYCG	2 cut(s)	103, 2778
BshFI	GGCC	8 cut(s)	8, 80, 102, 324, 863, 1810, 2037, 2600
BshVI	ATCGAT	1 cut(s)	2539
BsiEI	CGRYCG	2 cut(s)	103, 2778
BsiHKAI	GWGCWC	3 cut(s)	32, 182, 683
BsiHKCI	CYCGRG	1 cut(s)	59
BsiSI	CCGG	7 cut(s)	60, 445, 492, 1856, 2331, 2435, 2707
BslFI	GGGAC	1 cut(s)	2731
BslI	CCNNNNNNNGG	7 cut(s)	713, 988, 1317, 1942, 2099, 2191, 2423
BsmAI	GTCTC	3 cut(s)	6, 1528, 2087
BsmFI	GGGAC	1 cut(s)	2731
BsmI	GAATGC	2 cut(s)	644, 2024
BsnI	GGCC	8 cut(s)	8, 80, 102, 324, 863, 1810, 2037, 2600
Bso31I	GGTCTC	1 cut(s)	2087
BsoBI	CYCGRG	1 cut(s)	59
Bsp119I	TTCGAA	1 cut(s)	1498
Bsp1286I	GDGCHC	5 cut(s)	32, 127, 182, 358, 683
Bsp1407I	TGTACA	1 cut(s)	1439
Bsp1720I	GCTNAGC	1 cut(s)	2363
Bsp19I	CCATGG	1 cut(s)	2511
BspANI	GGCC	8 cut(s)	8, 80, 102, 324, 863, 1810, 2037, 2600
BspCNI	CTCAG	5 cut(s)	139, 781, 1188, 2726, 2737
BspDI	ATCGAT	1 cut(s)	2539
BspFNI	CGCG	3 cut(s)	93, 437, 2801
BspHI	TCATGA	3 cut(s)	1203, 1867, 2647
BspLI	GGNNCC	3 cut(s)	357, 1309, 2316
BspMAI	CTGCAG	1 cut(s)	2869
BspMI	ACCTGC	1 cut(s)	476
BspQI	GCTCTTC	1 cut(s)	1154
BspT104I	TTCGAA	1 cut(s)	1498
BspTI	CTTAAG	1 cut(s)	2237
BspTNI	GGTCTC	1 cut(s)	2087
BsrFI	RCCGGY	2 cut(s)	491, 2706
BsrGI	TGTACA	1 cut(s)	1439
BsrI	ACTGG	6 cut(s)	314, 502, 1354, 1477, 2209, 2428
BssAI	RCCGGY	2 cut(s)	491, 2706
BssECI	CCNNGG	4 cut(s)	58, 59, 1951, 2511
BssT1I	CCWWGG	1 cut(s)	2511
Bst2UI	CCWGG	5 cut(s)	207, 941, 1081, 1560, 1953
Bst4CI	ACNGT	6 cut(s)	481, 907, 1270, 1444, 1599, 2776
Bst6I	CTCTTC	5 cut(s)	1127, 1154, 1951, 2282, 2511
BstAFI	CTTAAG	1 cut(s)	2237
BstAPI	GCANNNNNTGC	1 cut(s)	998
BstAUI	TGTACA	1 cut(s)	1439
BstBI	TTCGAA	1 cut(s)	1498
BstC8I	GCNNGC	5 cut(s)	115, 265, 962, 2030, 2268
BstDEI	CTNAG	7 cut(s)	126, 789, 1196, 1527, 2363, 2713, 2745
BstDSI	CCRYGG	1 cut(s)	2511
BstEII	GGTNACC	1 cut(s)	2387
BstENI	CCTNNNNNAGG	1 cut(s)	1940
BstFNI	CGCG	3 cut(s)	93, 437, 2801
BstH2I	RGCGCY	1 cut(s)	979
BstHHI	GCGC	2 cut(s)	623, 978
BstMAI	GTCTC	3 cut(s)	6, 1528, 2087
BstMCI	CGRYCG	2 cut(s)	103, 2778
BstNI	CCWGG	5 cut(s)	207, 941, 1081, 1560, 1953
BstNSI	RCATGY	3 cut(s)	1288, 2032, 2683
BstPAI	GACNNNNGTC	1 cut(s)	291
BstPI	GGTNACC	1 cut(s)	2387
BstSCI	CCNGG	8 cut(s)	58, 59, 205, 939, 1079, 1558, 1951, 2330
BstSFI	CTRYAG	2 cut(s)	1345, 2865
BstUI	CGCG	3 cut(s)	93, 437, 2801
BstV1I	GCAGC	7 cut(s)	108, 117, 682, 951, 969, 2169, 2721
BstV2I	GAAGAC	1 cut(s)	2060
BstX2I	RGATCY	1 cut(s)	1829
BstXI	CCANNNNNNTGG	1 cut(s)	351
BstYI	RGATCY	1 cut(s)	1829
BstZI	CGGCCG	1 cut(s)	100
Bsu15I	ATCGAT	1 cut(s)	2539
BsuI	GTATCC	2 cut(s)	1566, 1900
BsuRI	GGCC	8 cut(s)	8, 80, 102, 324, 863, 1810, 2037, 2600
BsuTUI	ATCGAT	1 cut(s)	2539
BtgI	CCRYGG	1 cut(s)	2511
BtgZI	GCGATG	1 cut(s)	1645
BtsI	GCAGTG	4 cut(s)	683, 1401, 1989, 2576
BtsIMutI	CAGTG	6 cut(s)	477, 683, 1401, 1989, 2198, 2576
BveI	ACCTGC	1 cut(s)	476
Cac8I	GCNNGC	5 cut(s)	115, 265, 962, 2030, 2268
CciI	TCATGA	3 cut(s)	1203, 1867, 2647
CciNI	GCGGCCGC	1 cut(s)	100
CfoI	GCGC	2 cut(s)	623, 978
Cfr10I	RCCGGY	2 cut(s)	491, 2706
Cfr13I	GGNCC	3 cut(s)	323, 1115, 2036
Cfr9I	CCCGGG	1 cut(s)	59
ClaI	ATCGAT	1 cut(s)	2539
CseI	GACGC	4 cut(s)	426, 443, 2143, 2728
Csp6I	GTAC	5 cut(s)	913, 1440, 1600, 1948, 2421
CviQI	GTAC	5 cut(s)	913, 1440, 1600, 1948, 2421
DdeI	CTNAG	7 cut(s)	126, 789, 1196, 1527, 2363, 2713, 2745
DraI	TTTAAA	1 cut(s)	1017
DriI	GACNNNNNGTC	1 cut(s)	502
EaeI	YGGCCR	3 cut(s)	6, 78, 100
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	5 cut(s)	1127, 1154, 1951, 2282, 2511
Eam1105I	GACNNNNNGTC	1 cut(s)	502
EarI	CTCTTC	5 cut(s)	1127, 1154, 1951, 2282, 2511
EciI	GGCGGA	1 cut(s)	57
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	1 cut(s)	2511
Eco147I	AGGCCT	1 cut(s)	1810
Eco24I	GRGCYC	2 cut(s)	127, 358
Eco31I	GGTCTC	1 cut(s)	2087
Eco47I	GGWCC	1 cut(s)	1115
Eco52I	CGGCCG	1 cut(s)	100
Eco57I	CTGAAG	2 cut(s)	992, 2615
Eco88I	CYCGRG	1 cut(s)	59
Eco91I	GGTNACC	1 cut(s)	2387
EcoNI	CCTNNNNNAGG	1 cut(s)	1940
EcoO109I	RGGNCCY	1 cut(s)	323
EcoO65I	GGTNACC	1 cut(s)	2387
EcoRI	GAATTC	3 cut(s)	22, 53, 559
EcoRII	CCWGG	5 cut(s)	205, 939, 1079, 1558, 1951
EcoT14I	CCWWGG	1 cut(s)	2511
EcoT22I	ATGCAT	1 cut(s)	1218
EcoT38I	GRGCYC	2 cut(s)	127, 358
ErhI	CCWWGG	1 cut(s)	2511
FaqI	GGGAC	1 cut(s)	2731
FauI	CCCGC	2 cut(s)	960, 2041
FauNDI	CATATG	1 cut(s)	712
FbaI	TGATCA	1 cut(s)	1536
FblI	GTMKAC	1 cut(s)	2403
FriOI	GRGCYC	2 cut(s)	127, 358
FspBI	CTAG	8 cut(s)	584, 618, 740, 1094, 1812, 1833, 2010, 2418
GlaI	GCGC	2 cut(s)	622, 977
GsuI	CTGGAG	2 cut(s)	331, 2855
HaeII	RGCGCY	1 cut(s)	979
HaeIII	GGCC	8 cut(s)	8, 80, 102, 324, 863, 1810, 2037, 2600
HapII	CCGG	7 cut(s)	60, 445, 492, 1856, 2331, 2435, 2707
HgaI	GACGC	4 cut(s)	426, 443, 2143, 2728
HhaI	GCGC	2 cut(s)	623, 978
Hin6I	GCGC	2 cut(s)	621, 976
HinP1I	GCGC	2 cut(s)	621, 976
HincII	GTYRAC	1 cut(s)	847
HindII	GTYRAC	1 cut(s)	847
HindIII	AAGCTT	2 cut(s)	759, 922
HinfI	GANTC	9 cut(s)	404, 409, 731, 985, 1200, 1517, 1581, 1840, 2279
HpaI	GTTAAC	1 cut(s)	847
HpaII	CCGG	7 cut(s)	60, 445, 492, 1856, 2331, 2435, 2707
HphI	GGTGA	9 cut(s)	287, 457, 481, 1238, 2027, 2077, 2399, 2676, 2683
Hpy166II	GTNNAC	6 cut(s)	847, 1118, 1580, 1948, 2174, 2404
Hpy8I	GTNNAC	6 cut(s)	847, 1118, 1580, 1948, 2174, 2404
Hpy99I	CGWCG	1 cut(s)	2558
HpyAV	CCTTC	8 cut(s)	427, 806, 835, 1016, 2195, 2202, 2352, 2590
HpyCH4III	ACNGT	6 cut(s)	481, 907, 1270, 1444, 1599, 2776
HpyCH4IV	ACGT	4 cut(s)	462, 605, 900, 2460
HpyF3I	CTNAG	7 cut(s)	126, 789, 1196, 1527, 2363, 2713, 2745
HpySE526I	ACGT	4 cut(s)	462, 605, 900, 2460
HspAI	GCGC	2 cut(s)	621, 976
Ksp22I	TGATCA	1 cut(s)	1536
KspAI	GTTAAC	1 cut(s)	847
LguI	GCTCTTC	1 cut(s)	1154
LmnI	GCTCC	8 cut(s)	35, 147, 361, 686, 983, 1313, 2212, 2590
Lsp1109I	GCAGC	7 cut(s)	108, 117, 682, 951, 969, 2169, 2721
MaeI	CTAG	8 cut(s)	584, 618, 740, 1094, 1812, 1833, 2010, 2418
MaeII	ACGT	4 cut(s)	462, 605, 900, 2460
MflI	RGATCY	1 cut(s)	1829
MhlI	GDGCHC	5 cut(s)	32, 127, 182, 358, 683
MlsI	TGGCCA	1 cut(s)	8
MluI	ACGCGT	1 cut(s)	435
MluNI	TGGCCA	1 cut(s)	8
MlyI	GAGTC	3 cut(s)	398, 740, 1575
MmeI	TCCRAC	3 cut(s)	156, 490, 900
Mox20I	TGGCCA	1 cut(s)	8
Mph1103I	ATGCAT	1 cut(s)	1218
MscI	TGGCCA	1 cut(s)	8
MseI	TTAA	8 cut(s)	155, 846, 926, 1016, 1322, 1431, 2238, 2261
MslI	CAYNNNNRTG	2 cut(s)	349, 1034
Msp20I	TGGCCA	1 cut(s)	8
MspA1I	CMGCKG	4 cut(s)	99, 267, 361, 673
MspCI	CTTAAG	1 cut(s)	2237
MspI	CCGG	7 cut(s)	60, 445, 492, 1856, 2331, 2435, 2707
MspR9I	CCNGG	8 cut(s)	60, 61, 207, 941, 1081, 1560, 1953, 2332
Mva1269I	GAATGC	2 cut(s)	644, 2024
MvaI	CCWGG	5 cut(s)	207, 941, 1081, 1560, 1953
MvnI	CGCG	3 cut(s)	93, 437, 2801
NciI	CCSGG	3 cut(s)	60, 61, 2332
NcoI	CCATGG	1 cut(s)	2511
NdeI	CATATG	1 cut(s)	712
NlaIV	GGNNCC	3 cut(s)	357, 1309, 2316
NmuCI	GTSAC	9 cut(s)	275, 284, 463, 487, 1646, 1913, 1928, 2387, 2682
NotI	GCGGCCGC	1 cut(s)	100
NsiI	ATGCAT	1 cut(s)	1218
NspI	RCATGY	3 cut(s)	1288, 2032, 2683
NspV	TTCGAA	1 cut(s)	1498
PaeI	GCATGC	1 cut(s)	2032
PagI	TCATGA	3 cut(s)	1203, 1867, 2647
PaqCI	CACCTGC	1 cut(s)	476
PceI	AGGCCT	1 cut(s)	1810
PciI	ACATGT	1 cut(s)	2679
PciSI	GCTCTTC	1 cut(s)	1154
PcsI	WCGNNNNNNNCGW	2 cut(s)	188, 2633
PctI	GAATGC	2 cut(s)	644, 2024
PfeI	GAWTC	6 cut(s)	409, 985, 1200, 1517, 1840, 2279
PflMI	CCANNNNNTGG	2 cut(s)	713, 2099
PleI	GAGTC	3 cut(s)	398, 739, 1575
PpsI	GAGTC	3 cut(s)	398, 739, 1575
PscI	ACATGT	1 cut(s)	2679
PshAI	GACNNNNGTC	1 cut(s)	291
PsiI	TTATAA	1 cut(s)	2465
Psp1406I	AACGTT	1 cut(s)	2460
Psp6I	CCWGG	5 cut(s)	205, 939, 1079, 1558, 1951
PspEI	GGTNACC	1 cut(s)	2387
PspGI	CCWGG	5 cut(s)	205, 939, 1079, 1558, 1951
PspN4I	GGNNCC	3 cut(s)	357, 1309, 2316
PspPI	GGNCC	3 cut(s)	323, 1115, 2036
PstI	CTGCAG	1 cut(s)	2869
PsuI	RGATCY	1 cut(s)	1829
RsaI	GTAC	5 cut(s)	914, 1441, 1601, 1949, 2422
RsaNI	GTAC	5 cut(s)	913, 1440, 1600, 1948, 2421
RseI	CAYNNNNRTG	2 cut(s)	349, 1034
SapI	GCTCTTC	1 cut(s)	1154
SaqAI	TTAA	8 cut(s)	155, 846, 926, 1016, 1322, 1431, 2238, 2261
Sau96I	GGNCC	3 cut(s)	323, 1115, 2036
ScaI	AGTACT	1 cut(s)	2422
SchI	GAGTC	3 cut(s)	398, 740, 1575
ScrFI	CCNGG	8 cut(s)	60, 61, 207, 941, 1081, 1560, 1953, 2332
SduI	GDGCHC	5 cut(s)	32, 127, 182, 358, 683
SfcI	CTRYAG	2 cut(s)	1345, 2865
SfuI	TTCGAA	1 cut(s)	1498
SgrAI	CRCCGGYG	1 cut(s)	491
SinI	GGWCC	1 cut(s)	1115
SmaI	CCCGGG	1 cut(s)	61
SmiMI	CAYNNNNRTG	2 cut(s)	349, 1034
SmlI	CTYRAG	2 cut(s)	34, 2237
SmoI	CTYRAG	2 cut(s)	34, 2237
SphI	GCATGC	1 cut(s)	2032
SseBI	AGGCCT	1 cut(s)	1810
SspMI	CTAG	8 cut(s)	584, 618, 740, 1094, 1812, 1833, 2010, 2418
StuI	AGGCCT	1 cut(s)	1810
StyD4I	CCNGG	8 cut(s)	58, 59, 205, 939, 1079, 1558, 1951, 2330
StyI	CCWWGG	1 cut(s)	2511
TaaI	ACNGT	6 cut(s)	481, 907, 1270, 1444, 1599, 2776
TaiI	ACGT	4 cut(s)	465, 608, 903, 2463
TatI	WGTACW	2 cut(s)	1439, 2420
TauI	GCSGC	6 cut(s)	96, 102, 105, 383, 676, 863
TfiI	GAWTC	6 cut(s)	409, 985, 1200, 1517, 1840, 2279
Tru1I	TTAA	8 cut(s)	155, 846, 926, 1016, 1322, 1431, 2238, 2261
Tru9I	TTAA	8 cut(s)	155, 846, 926, 1016, 1322, 1431, 2238, 2261
TscAI	CASTG	6 cut(s)	484, 683, 1401, 1989, 2205, 2576
TseFI	GTSAC	9 cut(s)	275, 284, 463, 487, 1646, 1913, 1928, 2387, 2682
TseI	GCWGC	7 cut(s)	96, 105, 670, 957, 964, 2157, 2734
Tsp45I	GTSAC	9 cut(s)	275, 284, 463, 487, 1646, 1913, 1928, 2387, 2682
TspDTI	ATGAA	9 cut(s)	774, 1223, 1360, 1530, 2105, 2156, 2366, 2836, 2880
TspGWI	ACGGA	1 cut(s)	2322
TspMI	CCCGGG	1 cut(s)	59
TspRI	CASTG	6 cut(s)	484, 683, 1401, 1989, 2205, 2576
Van91I	CCANNNNNTGG	2 cut(s)	713, 2099
Vha464I	CTTAAG	1 cut(s)	2237
VpaK11BI	GGWCC	1 cut(s)	1115
XagI	CCTNNNNNAGG	1 cut(s)	1940
XapI	RAATTY	7 cut(s)	22, 53, 516, 559, 893, 1058, 1976
XceI	RCATGY	3 cut(s)	1288, 2032, 2683
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2509
XmaI	CCCGGG	1 cut(s)	59
XmiI	GTMKAC	1 cut(s)	2403
XspI	CTAG	8 cut(s)	584, 618, 740, 1094, 1812, 1833, 2010, 2418
ZrmI	AGTACT	1 cut(s)	2422
Zsp2I	ATGCAT	1 cut(s)	1218

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.