Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0000814

Physical Location & Seq

Genomic Coordinates Forward (+)

57058565 .. 57062179

Gene Structure 3615 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0436100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2535 bp

ATGGCGGAAGATAACTCAGAGTCATTGGTGGTGTACCTTCACGGCAACCTGGATTTGAAAATCATAGAGGCGCGATATTTGCCCAACATGGACATGCTCTCCGAGCGATTCCGGCGATTGTTCTCGGCCTGCCGCAAGCCATTCTCGAGCGCTAAACAGCACCGCAGTCACCATAAAATCATCACCAGCGACCCTTATGTCACGGTCTGCCTCGCCGGAGCTACCGTCGCCAGGACGCGCGTGATCTCCAACTCCCAGAACCCGGAATGGAAGGAGCATTTCAAGATCCCTCTCGCCCACCCCGTCTCGCAAGTCGAGTTCTACGTCAAAGACAACGACATGTTCGGCGCCGATTTAATCGGCGTCGCGTCAGTCCCGGCGCAGCGAATCCTCTCCGGCGAGACGATCAGCGATTGGTTTCCGATAATCGGGCCGTTGGGGAAGCCGCCGAAACCGGACTCGGCGGTTAGGTTGGAAATGAGGTTCACGAAATGCGAGGACAATCCGTTGTATCAGCACGGAATATCCCACGAACGTTTCGGGGGAGTCGAGAATTGTTACTTTCCGGCACGGCGAGGAGGTCACGTGACGCTGTACCAGGACGCGCACGTGCCGGACTCGGTGATGGAGGACATAGAATTGGACGACGGGGTTCAGTTTGAGCATGGGAAGTGCTGGGAGGATATATGTCACGCAATCTTGGAGGCCCATCATTTGGTGTACATTGTGGGCTGGTCCATTTATCACAAGGTCAAGGTGGTCAGAGAGCCCAGTAAGCCCTTGCCCAGCGGCGGCAATTTGAATTTGGGGGAGTTGCTCAAGTATAAATCCCAGGAAGGGTTGAGAGTTTTGTTGCTGGTTTGGGATGATAAGACTTCTCACAGCAAGTTCTTCATCAATACGACCGGAGTGATGCAAACGCATGATGAAGAAACCAGGAAGTTTTTTAAGCATTCTTCGGTGTCATGCGTGCTGTCACCGCGATATGCCAGCAGTAAGCTTAGCATTTTCAAGCAACAGGTTGTTGGGACCTTGTTTACGCATCATCAAAAGTGTGTGATTGTGGATACACAAGCGTCTGGAAACAATCGAAAGATTACTGCATTTATAGGAGGTCTGGACCTCTGTGATGGCCGCTATGATACTCCTGAGCATCGGCTGTTTCGCGATGTTGACACAGTTTTCCAGGGTGATTTCCATAATCCCACATTGGCTGGTACAAAGGGTCCAAGACAACCCTGGCATGATCTACATTGCAAAATTGAAGGGCCGGCTGCATACGATGTGCTAACTAATTTTGAGCAGCGTTGGAAAAGAGCCACAAAATGGTCAGAGTTGGGCCAACGCTTTAAAAGAGTGTCGCGTTGGCACGACGATGCTTTGATCAAGTTGGAGCGCATCTCATGGATACTTAGTCCCGCACCAAAAAGTTCCAATGATGATCCTGTATTACGGGTTTCAGATGAAGATGATCCTCAAAACTGGCGTGTTCAGATATTCCGATCCATAGATTCTGGATCTGTGAAAGGATTTCCCAAAGATGTCTATGAAGCTGAGAATCAGAATCTTTTCTGTGCCAAAAATTTGGTTATAGACAAGAGCATCCAAACAGCATATATCGAAGCAATCAGATCTGCGCAACACTTCATATACATTGAGAATCAGTATTTCCTCGGATCATCTTATGCATGGCCAACTTACAAAGAAGCAGGTGCTGACAATTTAATTCCAATGGAGTTGGCATTAAAGATTGCTAGTAAGATACGAGCCAAAGAGAGATTTGCAGTTTATGTCGTGATACCAATGTGGCCAGAAGGTGTGCCCTCTTCTGCTCCTGTGCAACAAATTCTCTTTTGGCAGGGACAAACAATGCAAATGATGTATGAAATCATAGCAAAAGAGCTGAAATCCATGAATATTGCGAATGCTCATCCGCAGGACTACCTAAATTTCTACTGTCTTGGTAATCGGGAAAAGCTGCCTGCCACAGTGTCCTGTACAAATAACCAAGCTCCCAGAACTGGTTCTCCAAGTCACACGGTTTCCGCCTCCCAAAAATTTCAACGGTTTATGATTTATGTTCATGCCAAGGGAATGGTAGTAGATGATGAGTACGTGATAATAGGTTCTGCGAATATCAACCAACGATCCATGGCAGGATCTAGGGACACCGAGATAGCCATGGGGGCATATCAACCGCATCACACGTGGGGTAAGAAGAAGCGGCATCCTCATGGCCAGGTATATGGATATAGAATGTCACTTTGGGCAGAACACATGGGGATGGTTGAGAATTGCTTCAAGGAGCCTCAAACATTAGAATGTGTGAAGCGTGTGAACGACATTGGTCAGGATAATTGGAGTAGGTTCGTGGCCGATGATTTTATAGAATTGCAGGGACACCTCATCAAATACCCAGTTGAGGTTTATGCCAATGGGAAGGTAGGTGCATTGCCTGGACGAGAGAGCTTCCCTGATGTTGGCGGTAAGATCCTCGGCGCCCGCACTAACCTTCCTGATGCTTTAACTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0006869 GO:0008150 GO:0009506 GO:0010876 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0030054 GO:0033036 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071702 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane_3 ABC_tran

Protein Analysis

844

Amino Acids

95.87

Weight (kDa)

7.64

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	16 - 141	7e-18	C2 domain
PLDc_2	PF13091	689 - 728	1.9e-06	PLD-like domain
PLDc	PF00614	692 - 717	2.5e-08	Phospholipase D Active site motif
PLD_C	PF12357	764 - 834	1.3e-28	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47740 AT3G47750 AT3G47760 AT3G47760 AT3G47760 AT3G47770 AT3G47770 AT3G47770 AT3G47780 AT3G47790 AT3G47790 AT3G47790 AT5G61700 AT5G61700 AT5G61700 AT5G61700 AT5G61740 AT5G61740 AT5G61740
fragaria_vesca	FvH4_5g15270 FvH4_5g15270 FvH4_5g15270 FvH4_5g15270 FvH4_5g17020
malus_domestica	MD00G1160300.v1.1 MD06G1189700.v1.1 MD14G1196600.v1.1
prunus_persica	Prupe.5G188500_v2.0.a1 Prupe.5G188500_v2.0.a1 Prupe.5G188700_v2.0.a1 Prupe.5G229600_v2.0.a1 Prupe.5G229600_v2.0.a1
pyrus_communis	pycom06g16850 pycom14g16330
rosa_chinensis	RchiOBHm_Chr7g0178831 RchiOBHm_Chr7g0180791 RchiOBHm_Chr7g0180811
rosa_laevigata	RLG00000005228 RLG00000005229 RLG00000005411
rosa_multiflora	Rmu_sc0007457.1_g000007 Rmu_sc0007457.1_g000009 Rmu_sc0025325.1_g000002 Rmu_sc0025325.1_g000004
rosa_roxburghii	Rroxscaffold_3G00272510 Rroxscaffold_3G00272520 Rroxscaffold_3G00274540
rosa_rugosa	Rorug01G0115100 Rorug06G0420100 Rorug06G0420100 Rorug06G0436000 Rorug06G0436100 Rorug06G0436200 Rorug06G0436300
rosa_samantha	Rh1BG107000 Rh7AG019400 Rh7AG037800 Rh7AG038000 Rh7BG018900 Rh7BG037600 Rh7BG037800 Rh7CG019500 Rh7CG039200 Rh7CG039300 Rh7DG019300
rosa_wichuraiana	Rw7G001580 Rw7G003120 Rw7G003130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1700
AasI	GACNNNNNNGTC	1 cut(s)	197
Acc16I	TGCGCA	1 cut(s)	1638
Acc36I	ACCTGC	1 cut(s)	1700
AccB1I	GGYRCC	2 cut(s)	347, 2498
AccB7I	CCANNNNNTGG	2 cut(s)	715, 1326
AccII	CGCG	8 cut(s)	73, 238, 240, 368, 605, 982, 1167, 1363
AclI	AACGTT	1 cut(s)	535
AclWI	GGATC	9 cut(s)	280, 1436, 1466, 1497, 1525, 1684, 2142, 2167, 2485
AcoI	YGGCCR	5 cut(s)	1132, 1691, 1808, 2236, 2373
AcsI	RAATTY	5 cut(s)	802, 1582, 1845, 1948, 2057
AcvI	CACGTG	3 cut(s)	586, 610, 2208
AcyI	GRCGYC	3 cut(s)	348, 363, 2499
AfaI	GTAC	6 cut(s)	35, 596, 722, 1219, 1999, 2114
AfeI	AGCGCT	1 cut(s)	151
AflIII	ACRYGT	2 cut(s)	339, 2205
AgsI	TTSAA	7 cut(s)	58, 283, 802, 1012, 1265, 2063, 2302
AjnI	CCWGG	9 cut(s)	48, 230, 597, 831, 935, 1185, 1238, 2238, 2455
AjuI	GAANNNNNNNTTGG	2 cut(s)	2248, 2280
AloI	GAACNNNNNNTCC	4 cut(s)	467, 499, 2011, 2043
AluBI	AGCT	7 cut(s)	221, 1000, 1553, 1903, 1978, 2012, 2469
AluI	AGCT	7 cut(s)	221, 1000, 1553, 1903, 1978, 2012, 2469
Alw26I	GTCTC	2 cut(s)	310, 395
AlwI	GGATC	9 cut(s)	280, 1436, 1466, 1497, 1525, 1684, 2142, 2167, 2485
AlwNI	CAGNNNCTG	1 cut(s)	1715
Ama87I	CYCGRG	1 cut(s)	145
Aor51HI	AGCGCT	1 cut(s)	151
ApeKI	GCWGC	4 cut(s)	382, 1274, 1303, 1978
ApoI	RAATTY	5 cut(s)	802, 1582, 1845, 1948, 2057
Asp700I	GAANNNNTTC	3 cut(s)	1568, 2023, 2297
AspLEI	GCGC	9 cut(s)	73, 152, 240, 350, 382, 607, 1398, 1639, 2501
AspS9I	GGNCC	8 cut(s)	431, 706, 735, 1029, 1120, 1226, 1268, 1339
AsuC2I	CCSGG	2 cut(s)	263, 377
AsuHPI	GGTGA	5 cut(s)	161, 175, 634, 969, 1202
AvaI	CYCGRG	1 cut(s)	145
AvaII	GGWCC	4 cut(s)	735, 1029, 1120, 1226
BaeGI	GKGCMC	1 cut(s)	1824
BaeI	ACNNNNGTAYC	2 cut(s)	1790, 1823
BalI	TGGCCA	3 cut(s)	1693, 1810, 2238
BanI	GGYRCC	2 cut(s)	347, 2498
BanII	GRGCYC	1 cut(s)	771
BbrPI	CACGTG	3 cut(s)	586, 610, 2208
BbvI	GCAGC	4 cut(s)	394, 1261, 1315, 1965
BccI	CCATC	4 cut(s)	619, 717, 1124, 2278
BceAI	ACGGC	3 cut(s)	58, 418, 587
BciT130I	CCWGG	9 cut(s)	50, 232, 599, 833, 937, 1187, 1240, 2240, 2457
BciVI	GTATCC	2 cut(s)	1060, 1401
BclI	TGATCA	1 cut(s)	1383
BcnI	CCSGG	2 cut(s)	263, 377
BcoDI	GTCTC	2 cut(s)	310, 395
BfaI	CTAG	2 cut(s)	1755, 2163
BfoI	RGCGCY	3 cut(s)	153, 351, 2502
BfuAI	ACCTGC	1 cut(s)	1700
BfuI	GTATCC	2 cut(s)	1060, 1401
BglI	GCCNNNNNGGC	1 cut(s)	2186
BglII	AGATCT	1 cut(s)	1631
BlpI	GCTNAGC	1 cut(s)	1001
Bme18I	GGWCC	4 cut(s)	735, 1029, 1120, 1226
BmeT110I	CYCGRG	1 cut(s)	145
BmgT120I	GGNCC	8 cut(s)	431, 706, 735, 1029, 1120, 1226, 1268, 1339
BmiI	GGNNCC	5 cut(s)	349, 1030, 1227, 2307, 2500
BmrI	ACTGGG	2 cut(s)	765, 2411
BmsI	GCATC	9 cut(s)	903, 1051, 1162, 1366, 1407, 1611, 2209, 2236, 2509
BmuI	ACTGGG	2 cut(s)	765, 2411
BoxI	GACNNNNGTC	1 cut(s)	2346
BplI	GAGNNNNNCTC	2 cut(s)	1385, 1417
Bpu10I	CCTNAGC	1 cut(s)	1149
Bpu1102I	GCTNAGC	1 cut(s)	1001
BpuEI	CTTGAG	1 cut(s)	803
BpuMI	CCSGG	2 cut(s)	263, 377
BsaAI	YACGTR	4 cut(s)	586, 610, 2116, 2208
BsaHI	GRCGYC	3 cut(s)	348, 363, 2499
BsaJI	CCNNGG	8 cut(s)	831, 1186, 1238, 1672, 2088, 2151, 2181, 2494
BsaWI	WCCGGW	2 cut(s)	454, 905
BsaXI	ACNNNNNCTCC	8 cut(s)	83, 113, 210, 240, 570, 600, 2011, 2041
Bse118I	RCCGGY	1 cut(s)	1270
Bse1I	ACTGG	4 cut(s)	771, 1487, 2026, 2417
Bse3DI	GCAATG	2 cut(s)	1252, 2450
BseBI	CCWGG	9 cut(s)	50, 232, 599, 833, 937, 1187, 1240, 2240, 2457
BseDI	CCNNGG	8 cut(s)	831, 1186, 1238, 1672, 2088, 2151, 2181, 2494
BseGI	GGATG	5 cut(s)	871, 1602, 1930, 2227, 2289
BseMI	GCAATG	2 cut(s)	1252, 2450
BseMII	CTCAG	3 cut(s)	30, 1140, 1545
BseNI	ACTGG	4 cut(s)	771, 1487, 2026, 2417
BseRI	GAGGAG	1 cut(s)	591
BseSI	GKGCMC	1 cut(s)	1824
BseXI	GCAGC	4 cut(s)	394, 1261, 1315, 1965
BseYI	CCCAGC	2 cut(s)	675, 785
Bsh1236I	CGCG	8 cut(s)	73, 238, 240, 368, 605, 982, 1167, 1363
Bsh1285I	CGRYCG	1 cut(s)	906
BshNI	GGYRCC	2 cut(s)	347, 2498
BsiEI	CGRYCG	1 cut(s)	906
BsiHKCI	CYCGRG	1 cut(s)	145
BslFI	GGGAC	6 cut(s)	359, 1042, 1401, 1875, 2180, 2412
BsmAI	GTCTC	2 cut(s)	310, 395
BsmBI	CGTCTC	2 cut(s)	310, 395
BsmFI	GGGAC	6 cut(s)	359, 1042, 1401, 1875, 2180, 2412
BsmI	GAATGC	2 cut(s)	952, 1930
BsoBI	CYCGRG	1 cut(s)	145
Bsp1286I	GDGCHC	2 cut(s)	771, 1824
Bsp1407I	TGTACA	2 cut(s)	720, 1997
Bsp1720I	GCTNAGC	1 cut(s)	1001
Bsp19I	CCATGG	2 cut(s)	2151, 2181
Bsp68I	TCGCGA	1 cut(s)	1167
BspCNI	CTCAG	3 cut(s)	29, 1141, 1546
BspFNI	CGCG	8 cut(s)	73, 238, 240, 368, 605, 982, 1167, 1363
BspLI	GGNNCC	5 cut(s)	349, 1030, 1227, 2307, 2500
BspMI	ACCTGC	1 cut(s)	1700
BspPI	GGATC	9 cut(s)	280, 1436, 1466, 1497, 1525, 1684, 2142, 2167, 2485
BspT107I	GGYRCC	2 cut(s)	347, 2498
BsrDI	GCAATG	2 cut(s)	1252, 2450
BsrFI	RCCGGY	1 cut(s)	1270
BsrGI	TGTACA	2 cut(s)	720, 1997
BsrI	ACTGG	4 cut(s)	771, 1487, 2026, 2417
BssAI	RCCGGY	1 cut(s)	1270
BssECI	CCNNGG	8 cut(s)	831, 1186, 1238, 1672, 2088, 2151, 2181, 2494
BssNI	GRCGYC	3 cut(s)	348, 363, 2499
BssT1I	CCWWGG	3 cut(s)	2088, 2151, 2181
Bst2UI	CCWGG	9 cut(s)	50, 232, 599, 833, 937, 1187, 1240, 2240, 2457
Bst4CI	ACNGT	7 cut(s)	205, 226, 1180, 1958, 1990, 2041, 2067
Bst6I	CTCTTC	1 cut(s)	1831
BstACI	GRCGYC	3 cut(s)	348, 363, 2499
BstAUI	TGTACA	2 cut(s)	720, 1997
BstBAI	YACGTR	4 cut(s)	586, 610, 2116, 2208
BstC8I	GCNNGC	7 cut(s)	130, 137, 971, 991, 1272, 1983, 2503
BstDEI	CTNAG	5 cut(s)	16, 1001, 1149, 1412, 1554
BstDSI	CCRYGG	2 cut(s)	2151, 2181
BstF5I	GGATG	5 cut(s)	871, 1602, 1930, 2227, 2289
BstFNI	CGCG	8 cut(s)	73, 238, 240, 368, 605, 982, 1167, 1363
BstH2I	RGCGCY	3 cut(s)	153, 351, 2502
BstHHI	GCGC	9 cut(s)	73, 152, 240, 350, 382, 607, 1398, 1639, 2501
BstMAI	GTCTC	2 cut(s)	310, 395
BstMCI	CGRYCG	1 cut(s)	906
BstMWI	GCNNNNNNNGC	7 cut(s)	79, 103, 112, 227, 775, 979, 2186
BstNI	CCWGG	9 cut(s)	50, 232, 599, 833, 937, 1187, 1240, 2240, 2457
BstNSI	RCATGY	2 cut(s)	97, 343
BstPAI	GACNNNNGTC	1 cut(s)	2346
BstSLI	GKGCMC	1 cut(s)	1824
BstUI	CGCG	8 cut(s)	73, 238, 240, 368, 605, 982, 1167, 1363
BstV1I	GCAGC	4 cut(s)	394, 1261, 1315, 1965
BstX2I	RGATCY	5 cut(s)	285, 1517, 1631, 2159, 2490
BstXI	CCANNNNNNTGG	3 cut(s)	1585, 2095, 2246
BstYI	RGATCY	5 cut(s)	285, 1517, 1631, 2159, 2490
BsuI	GTATCC	2 cut(s)	1060, 1401
BtgI	CCRYGG	2 cut(s)	2151, 2181
BtgZI	GCGATG	1 cut(s)	1182
BtsCI	GGATG	5 cut(s)	871, 1602, 1930, 2227, 2289
BtsIMutI	CAGTG	1 cut(s)	1995
BtuMI	TCGCGA	1 cut(s)	1167
BveI	ACCTGC	1 cut(s)	1700
Cac8I	GCNNGC	7 cut(s)	130, 137, 971, 991, 1272, 1983, 2503
CaiI	CAGNNNCTG	1 cut(s)	1715
CfoI	GCGC	9 cut(s)	73, 152, 240, 350, 382, 607, 1398, 1639, 2501
Cfr10I	RCCGGY	1 cut(s)	1270
Cfr13I	GGNCC	8 cut(s)	431, 706, 735, 1029, 1120, 1226, 1268, 1339
CseI	GACGC	6 cut(s)	244, 352, 357, 598, 611, 1065
Csp6I	GTAC	6 cut(s)	34, 595, 721, 1218, 1998, 2113
CviQI	GTAC	6 cut(s)	34, 595, 721, 1218, 1998, 2113
DdeI	CTNAG	5 cut(s)	16, 1001, 1149, 1412, 1554
DinI	GGCGCC	2 cut(s)	349, 2500
DraI	TTTAAA	1 cut(s)	1351
DrdI	GACNNNNNNGTC	1 cut(s)	197
DseDI	GACNNNNNNGTC	1 cut(s)	197
EaeI	YGGCCR	5 cut(s)	1132, 1691, 1808, 2236, 2373
Eam1104I	CTCTTC	1 cut(s)	1831
EarI	CTCTTC	1 cut(s)	1831
EciI	GGCGGA	2 cut(s)	20, 2035
Eco130I	CCWWGG	3 cut(s)	2088, 2151, 2181
Eco24I	GRGCYC	1 cut(s)	771
Eco47I	GGWCC	4 cut(s)	735, 1029, 1120, 1226
Eco47III	AGCGCT	1 cut(s)	151
Eco72I	CACGTG	3 cut(s)	586, 610, 2208
Eco88I	CYCGRG	1 cut(s)	145
EcoO109I	RGGNCCY	1 cut(s)	1029
EcoRII	CCWGG	9 cut(s)	48, 230, 597, 831, 935, 1185, 1238, 2238, 2455
EcoT14I	CCWWGG	3 cut(s)	2088, 2151, 2181
EcoT22I	ATGCAT	1 cut(s)	1690
EcoT38I	GRGCYC	1 cut(s)	771
EgeI	GGCGCC	2 cut(s)	349, 2500
EheI	GGCGCC	2 cut(s)	349, 2500
ErhI	CCWWGG	3 cut(s)	2088, 2151, 2181
Esp3I	CGTCTC	2 cut(s)	310, 395
FaqI	GGGAC	6 cut(s)	359, 1042, 1401, 1875, 2180, 2412
FauI	CCCGC	2 cut(s)	1426, 2510
FbaI	TGATCA	1 cut(s)	1383
FokI	GGATG	5 cut(s)	878, 1589, 1917, 2214, 2296
FriOI	GRGCYC	1 cut(s)	771
FspBI	CTAG	2 cut(s)	1755, 2163
FspI	TGCGCA	1 cut(s)	1638
GlaI	GCGC	9 cut(s)	72, 151, 239, 349, 381, 606, 1397, 1638, 2500
GsaI	CCCAGC	2 cut(s)	679, 789
HaeII	RGCGCY	3 cut(s)	153, 351, 2502
HgaI	GACGC	6 cut(s)	244, 352, 357, 598, 611, 1065
HhaI	GCGC	9 cut(s)	73, 152, 240, 350, 382, 607, 1398, 1639, 2501
Hin1I	GRCGYC	3 cut(s)	348, 363, 2499
Hin6I	GCGC	9 cut(s)	71, 150, 238, 348, 380, 605, 1396, 1637, 2499
HinP1I	GCGC	9 cut(s)	71, 150, 238, 348, 380, 605, 1396, 1637, 2499
HincII	GTYRAC	1 cut(s)	1174
HindII	GTYRAC	1 cut(s)	1174
HindIII	AAGCTT	1 cut(s)	998
HphI	GGTGA	5 cut(s)	161, 175, 634, 969, 1202
Hpy166II	GTNNAC	6 cut(s)	34, 486, 721, 1038, 1174, 2338
Hpy8I	GTNNAC	6 cut(s)	34, 486, 721, 1038, 1174, 2338
Hpy99I	CGWCG	4 cut(s)	230, 368, 650, 1376
HpyAV	CCTTC	7 cut(s)	47, 265, 830, 1259, 1808, 2434, 2522
HpyCH4III	ACNGT	7 cut(s)	205, 226, 1180, 1958, 1990, 2041, 2067
HpyCH4IV	ACGT	6 cut(s)	324, 535, 585, 609, 2115, 2207
HpyF10VI	GCNNNNNNNGC	7 cut(s)	79, 103, 112, 227, 775, 979, 2186
HpyF3I	CTNAG	5 cut(s)	16, 1001, 1149, 1412, 1554
HpySE526I	ACGT	6 cut(s)	324, 535, 585, 609, 2115, 2207
Hsp92I	GRCGYC	3 cut(s)	348, 363, 2499
HspAI	GCGC	9 cut(s)	71, 150, 238, 348, 380, 605, 1396, 1637, 2499
KasI	GGCGCC	2 cut(s)	347, 2498
KroI	GCCGGC	1 cut(s)	1270
KroNI	GCCGGC	1 cut(s)	1272
Ksp22I	TGATCA	1 cut(s)	1383
LmnI	GCTCC	6 cut(s)	218, 274, 1393, 1837, 2017, 2305
Lsp1109I	GCAGC	4 cut(s)	394, 1261, 1315, 1965
LweI	GCATC	9 cut(s)	903, 1051, 1162, 1366, 1407, 1611, 2209, 2236, 2509
MaeI	CTAG	2 cut(s)	1755, 2163
MaeII	ACGT	6 cut(s)	324, 535, 585, 609, 2115, 2207
MaeIII	GTNAC	9 cut(s)	167, 199, 557, 581, 586, 689, 975, 2033, 2259
MboII	GAAGA	7 cut(s)	20, 883, 941, 948, 1478, 1818, 2230
MflI	RGATCY	5 cut(s)	285, 1517, 1631, 2159, 2490
MhlI	GDGCHC	2 cut(s)	771, 1824
MlsI	TGGCCA	3 cut(s)	1693, 1810, 2238
MluNI	TGGCCA	3 cut(s)	1693, 1810, 2238
Mly113I	GGCGCC	2 cut(s)	348, 2499
MlyI	GAGTC	4 cut(s)	29, 452, 555, 611
MmeI	TCCRAC	4 cut(s)	273, 453, 1289, 1371
Mox20I	TGGCCA	3 cut(s)	1693, 1810, 2238
Mph1103I	ATGCAT	1 cut(s)	1690
MroNI	GCCGGC	1 cut(s)	1270
MroXI	GAANNNNTTC	3 cut(s)	1568, 2023, 2297
MscI	TGGCCA	3 cut(s)	1693, 1810, 2238
MseI	TTAA	7 cut(s)	356, 948, 1350, 1724, 1745, 2525, 2533
MslI	CAYNNNNRTG	5 cut(s)	92, 1374, 2232, 2282, 2320
Msp20I	TGGCCA	3 cut(s)	1693, 1810, 2238
MspA1I	CMGCKG	1 cut(s)	789
Mva1269I	GAATGC	2 cut(s)	952, 1930
MvaI	CCWGG	9 cut(s)	50, 232, 599, 833, 937, 1187, 1240, 2240, 2457
MvnI	CGCG	8 cut(s)	73, 238, 240, 368, 605, 982, 1167, 1363
MwoI	GCNNNNNNNGC	7 cut(s)	79, 103, 112, 227, 775, 979, 2186
NaeI	GCCGGC	1 cut(s)	1272
NarI	GGCGCC	2 cut(s)	348, 2499
NciI	CCSGG	2 cut(s)	263, 377
NcoI	CCATGG	2 cut(s)	2151, 2181
NgoMIV	GCCGGC	1 cut(s)	1270
NlaIV	GGNNCC	5 cut(s)	349, 1030, 1227, 2307, 2500
NmeAIII	GCCGAG	3 cut(s)	104, 440, 2475
NmuCI	GTSAC	8 cut(s)	167, 199, 581, 586, 689, 975, 2033, 2259
NruI	TCGCGA	1 cut(s)	1167
NsbI	TGCGCA	1 cut(s)	1638
NsiI	ATGCAT	1 cut(s)	1690
NspI	RCATGY	2 cut(s)	97, 343
PaeR7I	CTCGAG	1 cut(s)	145
PaqCI	CACCTGC	1 cut(s)	1700
PciI	ACATGT	1 cut(s)	339
PcsI	WCGNNNNNNNCGW	3 cut(s)	300, 321, 546
PctI	GAATGC	2 cut(s)	952, 1930
PdiI	GCCGGC	1 cut(s)	1272
PdmI	GAANNNNTTC	3 cut(s)	1568, 2023, 2297
PfeI	GAWTC	6 cut(s)	108, 387, 1511, 1558, 1564, 1660
PflMI	CCANNNNNTGG	2 cut(s)	715, 1326
PleI	GAGTC	4 cut(s)	28, 452, 554, 611
PluTI	GGCGCC	2 cut(s)	351, 2502
PmaCI	CACGTG	3 cut(s)	586, 610, 2208
PmlI	CACGTG	3 cut(s)	586, 610, 2208
PpsI	GAGTC	4 cut(s)	28, 452, 554, 611
Ppu21I	YACGTR	4 cut(s)	586, 610, 2116, 2208
PpuMI	RGGWCCY	1 cut(s)	1029
PscI	ACATGT	1 cut(s)	339
PshAI	GACNNNNGTC	1 cut(s)	2346
Psp1406I	AACGTT	1 cut(s)	535
Psp5II	RGGWCCY	1 cut(s)	1029
Psp6I	CCWGG	9 cut(s)	48, 230, 597, 831, 935, 1185, 1238, 2238, 2455
PspCI	CACGTG	3 cut(s)	586, 610, 2208
PspFI	CCCAGC	2 cut(s)	675, 785
PspGI	CCWGG	9 cut(s)	48, 230, 597, 831, 935, 1185, 1238, 2238, 2455
PspN4I	GGNNCC	5 cut(s)	349, 1030, 1227, 2307, 2500
PspPI	GGNCC	8 cut(s)	431, 706, 735, 1029, 1120, 1226, 1268, 1339
PspPPI	RGGWCCY	1 cut(s)	1029
PstNI	CAGNNNCTG	1 cut(s)	1715
PsuI	RGATCY	5 cut(s)	285, 1517, 1631, 2159, 2490
RruI	TCGCGA	1 cut(s)	1167
RsaI	GTAC	6 cut(s)	35, 596, 722, 1219, 1999, 2114
RsaNI	GTAC	6 cut(s)	34, 595, 721, 1218, 1998, 2113
RseI	CAYNNNNRTG	5 cut(s)	92, 1374, 2232, 2282, 2320
SaqAI	TTAA	7 cut(s)	356, 948, 1350, 1724, 1745, 2525, 2533
Sau96I	GGNCC	8 cut(s)	431, 706, 735, 1029, 1120, 1226, 1268, 1339
SchI	GAGTC	4 cut(s)	29, 452, 555, 611
SduI	GDGCHC	2 cut(s)	771, 1824
SfaNI	GCATC	9 cut(s)	903, 1051, 1162, 1366, 1407, 1611, 2209, 2236, 2509
SfoI	GGCGCC	2 cut(s)	349, 2500
Sfr274I	CTCGAG	1 cut(s)	145
SinI	GGWCC	4 cut(s)	735, 1029, 1120, 1226
SlaI	CTCGAG	1 cut(s)	145
SmiMI	CAYNNNNRTG	5 cut(s)	92, 1374, 2232, 2282, 2320
SmlI	CTYRAG	2 cut(s)	145, 818
SmoI	CTYRAG	2 cut(s)	145, 818
SspDI	GGCGCC	2 cut(s)	347, 2498
SspI	AATATT	1 cut(s)	1918
SspMI	CTAG	2 cut(s)	1755, 2163
StyI	CCWWGG	3 cut(s)	2088, 2151, 2181
TaaI	ACNGT	7 cut(s)	205, 226, 1180, 1958, 1990, 2041, 2067
TaiI	ACGT	6 cut(s)	327, 538, 588, 612, 2118, 2210
TaqI	TCGA	5 cut(s)	146, 315, 549, 1090, 1620
TatI	WGTACW	2 cut(s)	720, 1997
TauI	GCSGC	6 cut(s)	135, 448, 792, 795, 1137, 2227
TfiI	GAWTC	6 cut(s)	108, 387, 1511, 1558, 1564, 1660
Tru1I	TTAA	7 cut(s)	356, 948, 1350, 1724, 1745, 2525, 2533
Tru9I	TTAA	7 cut(s)	356, 948, 1350, 1724, 1745, 2525, 2533
TscAI	CASTG	1 cut(s)	1995
TseFI	GTSAC	8 cut(s)	167, 199, 581, 586, 689, 975, 2033, 2259
TseI	GCWGC	4 cut(s)	382, 1274, 1303, 1978
Tsp45I	GTSAC	8 cut(s)	167, 199, 581, 586, 689, 975, 2033, 2259
TspDTI	ATGAA	8 cut(s)	883, 942, 1479, 1563, 1636, 1899, 1928, 2072
TspGWI	ACGGA	2 cut(s)	495, 534
TspRI	CASTG	1 cut(s)	1995
Van91I	CCANNNNNTGG	2 cut(s)	715, 1326
VpaK11BI	GGWCC	4 cut(s)	735, 1029, 1120, 1226
XapI	RAATTY	5 cut(s)	802, 1582, 1845, 1948, 2057
XceI	RCATGY	2 cut(s)	97, 343
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1236
XhoI	CTCGAG	1 cut(s)	145
XmnI	GAANNNNTTC	3 cut(s)	1568, 2023, 2297
XspI	CTAG	2 cut(s)	1755, 2163
Zsp2I	ATGCAT	1 cut(s)	1690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.