Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000814

Physical Location & Seq

Genomic Coordinates Forward (+)

28689028 .. 28695111

Gene Structure 6084 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1196600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2838 bp

ATGGCGGATACATCCCATGGGCCTGCCAGCTTCTGGACTCAGGCCAATGCTCTGCTCAGAAAGAACCTAACTTTCCAGAAAAGAAATATCAAACAGAATATTCGGCTAGTTTCATTTCCAATTCTCCTGTGTCTATTGCTTGTTCTCATCCAATATTTGGTCAACAATGAACTGGATAAGCCCGAAAACAGGTGTGGTTGCAGTTGTGTTGATACAAATGGCGATGGCAAGTGTGAGAAAGTTTGTGGACTGGAATACTCAACTTTAACACAAGGGCCCAGTTGCCCGGTTCCTGATCCTCCACAATGGCCTCCATTGCTACAAGTGCCGGCTCCTGACTATCGCGCTGTTATGTCTGATGTTATTCCACATAAAGACTTGCCAAATGTGGCATGTAAGAGGTCAGGATCCTGTCCTGTAACTGTACTGTTCACGGGGAAAAATCAATCTCTCGGAGAAGTTTTGGCTGGGAGTATGTTCAGAAGTTCTTCTACTCTGAATTCATCTGATCTGGATTATTTAGCCCGTAGTGCTTTAGGATCAGAATCGACGCCTGACTATTCCAACTTCCTTGATCCGGCTTTCATTTCGGGTCTTCCCCTATATAGTGTTCAGAGCCAATGCTCACAAAACTCTACATTTTCTGTTCCAATTAACATATTATCCATCAAGATCCAACAAGAGGTTAGATGTGTTCAAGGTTTACATTTGTGGCGGGATAGTTCTTCTGAGATAAACAGTGAGCTGTATAAAGGTTACGAAAAGAGCAATTCAGAGAGGAAGATTAATGAAATACTTTCAGCCTATGACTTCTCTAACTCAAACGGGAACAATTTTAATGTAAGCATATGGTACAATTCAACATTTAAGAATGACATGGGAAGAGGTGCTATTGCTTTGATGCGGCTTCCACGCTCAGTGAATCTGGCATCCAATGCATACCTTCAGTTTGTACAAGGATTTGGTACTGAAATGCTGTTTGAGTTTGTGAAAGAAATGCCCAAGCCTGAAACCAAACTGAGGCTGGATTTTTCTTCTCTTCTTGGTACACTCTTCTTTACATGGGTCATTCTACAGCTGTTCCCGGTTGTTTTGACATCGCTGGTATACGAGAGGCAACAGAAACTGAGAATCATGATGAAAATGCATGGGCTTGGTGATGGGCCTTATTGGATGATTTCTTATACTTATTTTCTTACGGTATCTTCAATCTACATGCTGTGCTTTGTTATATTTGGCTCGGGTATAGGGTTAAAATTCTTCTCACTGAATGCCTACAGCATCCAATTTATATTTTACTTCATCTATATAAACCTGCAAATATCGCTGGCTTTTCTAGTAGCTGCAATGTTTTCAGACGTGAAGACTGCTACAGTTATAGGTTACATATTTGTCTTTGGAACTGGGCTTTTAGGAGGCTTTCTTTTTCAATTTTTTGTTCAAGATACATCATTCCCTAGTTGGATCATCGTTCTGGAACTATATCCTGGCTTCTCTTTATATCGTGGGTTATATGAGTTTGCACAATATTCATTTAACGGGAATTACATGGGGACTGATGGGATGCGGTGGGGAGATTTGAGTGATAGCAAGAATGGGATGACAGAGGTATTGATTATCATGGTTGTTGAGTGGTTTGTGGTGCTTCTTTTTGCATATTACGTAGATCAAGCTGTCTCATCAGGAACTGGGAAAGGTACTTTCTTTTGCTTCCAACGCTTTAGGAAGAAGAAACTGCCATCTCTTAGGATGCGTAGTTTGCAAAGGCAGGGATCTAAAGTTTCTATTGAGATGGAGAAACCTGATGTCTGTCAAGAGAGGGAGAAGGTTGAAAAATTGCTACTGGATTCAGATACAACTCATTCAGTCATCTGCGACAACCTCAAGAAGGTTTATCCTGGAAGAGATGGAAACCCAGAAAAATTTGCAGTGAGAGGATTGTCTCTTGCGTTGTCTCGAGGGGAGTGCTTTGGTATGCTTGGCCCTAATGGTGCTGGGAAGACCTCTTTTATCAGTATGATGATTGGTCTCACAAAGTCAACCTCTGGCACTGCATATGTTCAGGGTCTGGACATCCGGACTCAGATGGATGAAATATACACCAGCATGGGTGTTTGTCCACAGCATGACCTGTTATGGGAAACCCTGACAGGTAGGGAGCATGTGCTATTCTATGGAAGACTTAAGAACCTCAAAGGTTCTGTATTAACGCAAGCAGTGGAAGAATCTTTGAAAAGTGTCAATCTATTTCACGGTGGGGTTGCAGACAAACAAGCTGGAAAGTATAGTGGAGGCATGAAGAGAAGGCTTAGCGTTGCAATTTCACTTATAGGGGATCCCAAAGTTGTTTACATGGATGAGCCCAGTACTGGACTTGATCCAGCTTCAAGAAACAACTTATGGACTGCTGTGAAGCGTGCAAAACAAGACCGGGCAATCATCCTAACCACACATTCCATGGAAGAGGCGGAGGTTCTGTGTGATCGGTTAGGAATTTTTGTGGATGGTAGCTTGCAGTGCATAGGAAACCCAAAAGAGCTGAAAGCAAGATATGGAGGATCTTATGTGTTCACAATGACAACATCTTCGAATCACGAGCAAGAGGTGGAGAACTTGGTGCTGCGTCTCTCCCCAAGTGCTAACAGGATATACCATCTATCCGGAACCCAGAAGTTTGAGTTGCCAAAACACGAGGTCAGAATTACAGATGTGTTTGAAGCAGTTGAGAACGCAAAGAGCAGGTTCACAGTTTTCGCGTGGGGTCTAGCCGACACCACATTGGAGGATGTCTTCATCAAGGTTGCACTTGGGGCTCAAACCTCCAGTGAATTGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005911 GO:0006810 GO:0006869 GO:0008150 GO:0009506 GO:0010876 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0030054 GO:0033036 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071702 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane_3 ABC_tran

Protein Analysis

946

Amino Acids

105.69

Weight (kDa)

8.1

Isoelectric Point (pI)

34.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC2_membrane_3	PF12698	261 - 554	9.3e-14	ABC-2 family transporter protein
ABC_tran	PF00005	645 - 789	3.2e-24	ABC transporter
AAA_21	PF13304	760 - 821	1.8e-07	AAA domain, putative AbiEii toxin, Type IV TA system
ABCA12_C	PF24526	855 - 905	5.7e-30	ABCA12-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47740 AT3G47750 AT3G47760 AT3G47760 AT3G47760 AT3G47770 AT3G47770 AT3G47770 AT3G47780 AT3G47790 AT3G47790 AT3G47790 AT5G61700 AT5G61700 AT5G61700 AT5G61700 AT5G61740 AT5G61740 AT5G61740
fragaria_vesca	FvH4_5g15270 FvH4_5g15270 FvH4_5g15270 FvH4_5g15270 FvH4_5g17020
malus_domestica	MD00G1160300.v1.1 MD06G1189700.v1.1 MD14G1196600.v1.1
prunus_persica	Prupe.5G188500_v2.0.a1 Prupe.5G188500_v2.0.a1 Prupe.5G188700_v2.0.a1 Prupe.5G229600_v2.0.a1 Prupe.5G229600_v2.0.a1
pyrus_communis	pycom06g16850 pycom14g16330
rosa_chinensis	RchiOBHm_Chr7g0178831 RchiOBHm_Chr7g0180791 RchiOBHm_Chr7g0180811
rosa_laevigata	RLG00000005228 RLG00000005229 RLG00000005411
rosa_multiflora	Rmu_sc0007457.1_g000007 Rmu_sc0007457.1_g000009 Rmu_sc0025325.1_g000002 Rmu_sc0025325.1_g000004
rosa_roxburghii	Rroxscaffold_3G00272510 Rroxscaffold_3G00272520 Rroxscaffold_3G00274540
rosa_rugosa	Rorug01G0115100 Rorug06G0420100 Rorug06G0420100 Rorug06G0436000 Rorug06G0436100 Rorug06G0436200 Rorug06G0436300
rosa_samantha	Rh1BG107000 Rh7AG019400 Rh7AG037800 Rh7AG038000 Rh7BG018900 Rh7BG037600 Rh7BG037800 Rh7CG019500 Rh7CG039200 Rh7CG039300 Rh7DG019300
rosa_wichuraiana	Rw7G001580 Rw7G003120 Rw7G003130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1323, 2730
AccB7I	CCANNNNNTGG	2 cut(s)	33, 157
AccI	GTMKAC	1 cut(s)	1107
AccII	CGCG	2 cut(s)	345, 2756
AccIII	TCCGGA	2 cut(s)	2076, 2660
AciI	CCGC	5 cut(s)	5, 715, 904, 1567, 2468
AcsI	RAATTY	4 cut(s)	499, 1256, 1922, 2493
AcuI	CTGAAG	1 cut(s)	929
AcyI	GRCGYC	1 cut(s)	551
AfaI	GTAC	7 cut(s)	426, 854, 954, 967, 1048, 1699, 2368
AfiI	CCNNNNNNNGG	9 cut(s)	33, 157, 189, 577, 682, 1020, 1888, 2137, 2369
AflII	CTTAAG	1 cut(s)	2183
AgsI	TTSAA	9 cut(s)	698, 861, 1209, 1430, 1442, 1832, 2233, 2388, 2717
AjiI	CACGTC	1 cut(s)	1360
AjnI	CCWGG	2 cut(s)	1486, 1897
AluBI	AGCT	9 cut(s)	30, 745, 1078, 1343, 1673, 2276, 2384, 2511, 2539
AluI	AGCT	9 cut(s)	30, 745, 1078, 1343, 1673, 2276, 2384, 2511, 2539
Alw26I	GTCTC	5 cut(s)	1681, 1947, 1959, 2033, 2630
AlwNI	CAGNNNCTG	4 cut(s)	33, 1126, 1688, 2068
Ama87I	CYCGRG	2 cut(s)	1240, 1956
Aor13HI	TCCGGA	2 cut(s)	2076, 2660
AoxI	GGCC	6 cut(s)	20, 42, 275, 308, 1163, 1981
ApaI	GGGCCC	1 cut(s)	279
ApeKI	GCWGC	2 cut(s)	1343, 2620
ApoI	RAATTY	4 cut(s)	499, 1256, 1922, 2493
ArsI	GACNNNNNNTTYG	2 cut(s)	2569, 2601
AseI	ATTAAT	1 cut(s)	786
Asp700I	GAANNNNTTC	1 cut(s)	487
AspLEI	GCGC	1 cut(s)	347
AspS9I	GGNCC	5 cut(s)	20, 275, 276, 1163, 1982
AsuC2I	CCSGG	3 cut(s)	287, 1085, 2432
AsuHPI	GGTGA	1 cut(s)	1169
AsuII	TTCGAA	1 cut(s)	2588
AvaI	CYCGRG	2 cut(s)	1240, 1956
BaeGI	GKGCMC	1 cut(s)	279
BamHI	GGATCC	2 cut(s)	407, 2335
BanII	GRGCYC	3 cut(s)	279, 2364, 2815
BarI	GAAGNNNNNNTAC	2 cut(s)	475, 507
BauI	CACGAG	2 cut(s)	2594, 2690
BbsI	GAAGAC	5 cut(s)	587, 1370, 2006, 2185, 2782
BbvI	GCAGC	2 cut(s)	1330, 2607
BcgI	CGANNNNNNTGC	2 cut(s)	1947, 1981
BciT130I	CCWGG	2 cut(s)	1488, 1899
BcnI	CCSGG	3 cut(s)	287, 1085, 2432
BcoDI	GTCTC	5 cut(s)	1681, 1947, 1959, 2033, 2630
BfaI	CTAG	4 cut(s)	107, 1337, 1458, 2765
BfmI	CTRYAG	3 cut(s)	1073, 1276, 1371
BfrI	CTTAAG	1 cut(s)	2183
BfuAI	ACCTGC	2 cut(s)	1323, 2730
BisI	GCNGC	3 cut(s)	905, 1344, 2621
BlpI	GCTNAGC	1 cut(s)	2309
BlsI	GCNGC	3 cut(s)	906, 1345, 2622
BmcAI	AGTACT	1 cut(s)	2368
Bme1390I	CCNGG	5 cut(s)	287, 1085, 1488, 1899, 2432
BmeT110I	CYCGRG	2 cut(s)	1240, 1956
BmgBI	CACGTC	1 cut(s)	1360
BmgT120I	GGNCC	5 cut(s)	20, 275, 276, 1163, 1982
BmiI	GGNNCC	6 cut(s)	277, 291, 333, 409, 2337, 2665
BmrFI	CCNGG	5 cut(s)	287, 1085, 1488, 1899, 2432
BmrI	ACTGGG	4 cut(s)	273, 1413, 1698, 2358
BmsI	GCATC	5 cut(s)	891, 938, 1290, 1554, 1740
BmuI	ACTGGG	4 cut(s)	273, 1413, 1698, 2358
BpiI	GAAGAC	5 cut(s)	587, 1370, 2006, 2185, 2782
BpmI	CTGGAG	1 cut(s)	2806
Bpu1102I	GCTNAGC	1 cut(s)	2309
Bpu14I	TTCGAA	1 cut(s)	2588
BpuEI	CTTGAG	1 cut(s)	1868
BpuMI	CCSGG	3 cut(s)	287, 1085, 2432
BsaAI	YACGTR	1 cut(s)	1663
BsaBI	GATNNNNATC	1 cut(s)	544
BsaHI	GRCGYC	1 cut(s)	551
BsaI	GGTCTC	1 cut(s)	2033
BsaJI	CCNNGG	2 cut(s)	16, 2457
BsaWI	WCCGGW	2 cut(s)	2076, 2660
BsaXI	ACNNNNNCTCC	2 cut(s)	2462, 2492
Bsc4I	CCNNNNNNNGG	9 cut(s)	33, 157, 189, 577, 682, 1020, 1888, 2137, 2369
Bse118I	RCCGGY	1 cut(s)	328
Bse1I	ACTGG	9 cut(s)	177, 255, 279, 1408, 1693, 1848, 2364, 2374, 2823
Bse3DI	GCAATG	2 cut(s)	314, 1353
Bse8I	GATNNNNATC	1 cut(s)	544
BseAI	TCCGGA	2 cut(s)	2076, 2660
BseBI	CCWGG	2 cut(s)	1488, 1899
BseDI	CCNNGG	2 cut(s)	16, 2457
BseJI	GATNNNNATC	1 cut(s)	544
BseLI	CCNNNNNNNGG	9 cut(s)	33, 157, 189, 577, 682, 1020, 1888, 2137, 2369
BseMI	GCAATG	2 cut(s)	314, 1353
BseMII	CTCAG	7 cut(s)	53, 70, 720, 930, 1010, 1118, 2096
BseNI	ACTGG	9 cut(s)	177, 255, 279, 1408, 1693, 1848, 2364, 2374, 2823
BseSI	GKGCMC	1 cut(s)	279
BseXI	GCAGC	2 cut(s)	1330, 2607
BseYI	CCCAGC	2 cut(s)	467, 1994
Bsh1236I	CGCG	2 cut(s)	345, 2756
BshFI	GGCC	6 cut(s)	22, 44, 277, 310, 1165, 1983
BsiHKCI	CYCGRG	2 cut(s)	1240, 1956
BsiSI	CCGG	7 cut(s)	287, 329, 578, 1085, 2077, 2431, 2661
BslFI	GGGAC	1 cut(s)	1567
BslI	CCNNNNNNNGG	9 cut(s)	33, 157, 189, 577, 682, 1020, 1888, 2137, 2369
BsmAI	GTCTC	5 cut(s)	1681, 1947, 1959, 2033, 2630
BsmBI	CGTCTC	1 cut(s)	2630
BsmFI	GGGAC	1 cut(s)	1567
BsmI	GAATGC	1 cut(s)	1276
BsnI	GGCC	6 cut(s)	22, 44, 277, 310, 1165, 1983
Bso31I	GGTCTC	1 cut(s)	2033
BsoBI	CYCGRG	2 cut(s)	1240, 1956
Bsp119I	TTCGAA	1 cut(s)	2588
Bsp120I	GGGCCC	1 cut(s)	275
Bsp1286I	GDGCHC	3 cut(s)	279, 2364, 2815
Bsp13I	TCCGGA	2 cut(s)	2076, 2660
Bsp1407I	TGTACA	1 cut(s)	952
Bsp1720I	GCTNAGC	1 cut(s)	2309
Bsp19I	CCATGG	2 cut(s)	16, 2457
BspACI	CCGC	5 cut(s)	5, 715, 904, 1567, 2468
BspANI	GGCC	6 cut(s)	22, 44, 277, 310, 1165, 1983
BspCNI	CTCAG	7 cut(s)	52, 69, 721, 929, 1011, 1119, 2095
BspEI	TCCGGA	2 cut(s)	2076, 2660
BspFNI	CGCG	2 cut(s)	345, 2756
BspHI	TCATGA	1 cut(s)	1134
BspLI	GGNNCC	6 cut(s)	277, 291, 333, 409, 2337, 2665
BspMI	ACCTGC	2 cut(s)	1323, 2730
BspT104I	TTCGAA	1 cut(s)	2588
BspTI	CTTAAG	1 cut(s)	2183
BspTNI	GGTCTC	1 cut(s)	2033
BsrDI	GCAATG	2 cut(s)	314, 1353
BsrFI	RCCGGY	1 cut(s)	328
BsrGI	TGTACA	1 cut(s)	952
BsrI	ACTGG	9 cut(s)	177, 255, 279, 1408, 1693, 1848, 2364, 2374, 2823
BssAI	RCCGGY	1 cut(s)	328
BssECI	CCNNGG	2 cut(s)	16, 2457
BssNAI	GTATAC	1 cut(s)	1108
BssNI	GRCGYC	1 cut(s)	551
BssSI	CACGAG	2 cut(s)	2594, 2690
BssT1I	CCWWGG	2 cut(s)	16, 2457
Bst1107I	GTATAC	1 cut(s)	1108
Bst2BI	CACGAG	2 cut(s)	2594, 2690
Bst2UI	CCWGG	2 cut(s)	1488, 1899
Bst4CI	ACNGT	7 cut(s)	424, 429, 740, 1201, 1375, 2255, 2749
Bst6I	CTCTTC	6 cut(s)	877, 1044, 1058, 1897, 2294, 2457
BstACI	GRCGYC	1 cut(s)	551
BstAFI	CTTAAG	1 cut(s)	2183
BstAPI	GCANNNNNTGC	1 cut(s)	935
BstAUI	TGTACA	1 cut(s)	952
BstBAI	YACGTR	1 cut(s)	1663
BstBI	TTCGAA	1 cut(s)	2588
BstC8I	GCNNGC	7 cut(s)	24, 28, 330, 1329, 2214, 2418, 2513
BstDEI	CTNAG	9 cut(s)	39, 56, 729, 916, 1019, 1127, 1745, 2082, 2309
BstDSI	CCRYGG	2 cut(s)	16, 2457
BstENI	CCTNNNNNAGG	1 cut(s)	1886
BstFNI	CGCG	2 cut(s)	345, 2756
BstHHI	GCGC	1 cut(s)	347
BstMAI	GTCTC	5 cut(s)	1681, 1947, 1959, 2033, 2630
BstMWI	GCNNNNNNNGC	9 cut(s)	316, 325, 530, 935, 1324, 1716, 1759, 2517, 2810
BstNI	CCWGG	2 cut(s)	1488, 1899
BstNSI	RCATGY	3 cut(s)	396, 1219, 2165
BstSCI	CCNGG	5 cut(s)	285, 1083, 1486, 1897, 2430
BstSFI	CTRYAG	3 cut(s)	1073, 1276, 1371
BstSLI	GKGCMC	1 cut(s)	279
BstSNI	TACGTA	1 cut(s)	1663
BstUI	CGCG	2 cut(s)	345, 2756
BstV1I	GCAGC	2 cut(s)	1330, 2607
BstV2I	GAAGAC	5 cut(s)	587, 1370, 2006, 2185, 2782
BstX2I	RGATCY	5 cut(s)	407, 672, 1772, 2335, 2558
BstYI	RGATCY	5 cut(s)	407, 672, 1772, 2335, 2558
BstZ17I	GTATAC	1 cut(s)	1108
BsuRI	GGCC	6 cut(s)	22, 44, 277, 310, 1165, 1983
BtgI	CCRYGG	2 cut(s)	16, 2457
BtgZI	GCGATG	2 cut(s)	237, 1083
BtrI	CACGTC	1 cut(s)	1360
BtsI	GCAGTG	4 cut(s)	1935, 2049, 2223, 2522
BtsIMutI	CAGTG	8 cut(s)	745, 924, 1265, 1935, 2049, 2223, 2522, 2830
BveI	ACCTGC	2 cut(s)	1323, 2730
Cac8I	GCNNGC	7 cut(s)	24, 28, 330, 1329, 2214, 2418, 2513
CaiI	CAGNNNCTG	4 cut(s)	33, 1126, 1688, 2068
CciI	TCATGA	1 cut(s)	1134
CfoI	GCGC	1 cut(s)	347
Cfr10I	RCCGGY	1 cut(s)	328
Cfr13I	GGNCC	5 cut(s)	20, 275, 276, 1163, 1982
CseI	GACGC	2 cut(s)	559, 2612
Csp6I	GTAC	7 cut(s)	425, 853, 953, 966, 1047, 1698, 2367
CviQI	GTAC	7 cut(s)	425, 853, 953, 966, 1047, 1698, 2367
DdeI	CTNAG	9 cut(s)	39, 56, 729, 916, 1019, 1127, 1745, 2082, 2309
Eam1104I	CTCTTC	6 cut(s)	877, 1044, 1058, 1897, 2294, 2457
EarI	CTCTTC	6 cut(s)	877, 1044, 1058, 1897, 2294, 2457
EciI	GGCGGA	2 cut(s)	20, 2483
Eco105I	TACGTA	1 cut(s)	1663
Eco130I	CCWWGG	2 cut(s)	16, 2457
Eco24I	GRGCYC	3 cut(s)	279, 2364, 2815
Eco31I	GGTCTC	1 cut(s)	2033
Eco57I	CTGAAG	1 cut(s)	929
Eco88I	CYCGRG	2 cut(s)	1240, 1956
EcoNI	CCTNNNNNAGG	1 cut(s)	1886
EcoO109I	RGGNCCY	1 cut(s)	275
EcoRI	GAATTC	1 cut(s)	499
EcoRII	CCWGG	2 cut(s)	1486, 1897
EcoT14I	CCWWGG	2 cut(s)	16, 2457
EcoT22I	ATGCAT	2 cut(s)	940, 1149
EcoT38I	GRGCYC	3 cut(s)	279, 2364, 2815
ErhI	CCWWGG	2 cut(s)	16, 2457
Esp3I	CGTCTC	1 cut(s)	2630
FaqI	GGGAC	1 cut(s)	1567
FauI	CCCGC	1 cut(s)	708
FauNDI	CATATG	2 cut(s)	848, 2056
FblI	GTMKAC	1 cut(s)	1107
Fnu4HI	GCNGC	3 cut(s)	905, 1344, 2621
FriOI	GRGCYC	3 cut(s)	279, 2364, 2815
Fsp4HI	GCNGC	3 cut(s)	905, 1344, 2621
FspBI	CTAG	4 cut(s)	107, 1337, 1458, 2765
GlaI	GCGC	1 cut(s)	346
GluI	GCNGC	3 cut(s)	905, 1344, 2621
GsaI	CCCAGC	2 cut(s)	471, 1998
GsuI	CTGGAG	1 cut(s)	2806
HaeIII	GGCC	6 cut(s)	22, 44, 277, 310, 1165, 1983
HapII	CCGG	7 cut(s)	287, 329, 578, 1085, 2077, 2431, 2661
HgaI	GACGC	2 cut(s)	559, 2612
HhaI	GCGC	1 cut(s)	347
Hin1I	GRCGYC	1 cut(s)	551
Hin6I	GCGC	1 cut(s)	345
HinP1I	GCGC	1 cut(s)	345
HincII	GTYRAC	2 cut(s)	163, 2040
HindII	GTYRAC	2 cut(s)	163, 2040
HinfI	GANTC	8 cut(s)	37, 545, 922, 1131, 1847, 2080, 2225, 2590
HpaII	CCGG	7 cut(s)	287, 329, 578, 1085, 2077, 2431, 2661
HphI	GGTGA	1 cut(s)	1169
Hpy99I	CGWCG	1 cut(s)	553
HpyAV	CCTTC	4 cut(s)	953, 1819, 1882, 2298
HpyCH4III	ACNGT	7 cut(s)	424, 429, 740, 1201, 1375, 2255, 2749
HpyCH4IV	ACGT	2 cut(s)	1359, 1662
HpyF10VI	GCNNNNNNNGC	9 cut(s)	316, 325, 530, 935, 1324, 1716, 1759, 2517, 2810
HpyF3I	CTNAG	9 cut(s)	39, 56, 729, 916, 1019, 1127, 1745, 2082, 2309
HpySE526I	ACGT	2 cut(s)	1359, 1662
Hsp92I	GRCGYC	1 cut(s)	551
HspAI	GCGC	1 cut(s)	345
Kpn2I	TCCGGA	2 cut(s)	2076, 2660
KroI	GCCGGC	1 cut(s)	328
KroNI	GCCGGC	1 cut(s)	330
LmnI	GCTCC	2 cut(s)	337, 2158
Lsp1109I	GCAGC	2 cut(s)	1330, 2607
LweI	GCATC	5 cut(s)	891, 938, 1290, 1554, 1740
MaeI	CTAG	4 cut(s)	107, 1337, 1458, 2765
MaeII	ACGT	2 cut(s)	1359, 1662
MaeIII	GTNAC	3 cut(s)	418, 755, 1382
MflI	RGATCY	5 cut(s)	407, 672, 1772, 2335, 2558
MhlI	GDGCHC	3 cut(s)	279, 2364, 2815
MlyI	GAGTC	2 cut(s)	31, 2074
MmeI	TCCRAC	4 cut(s)	588, 700, 1442, 1738
Mph1103I	ATGCAT	2 cut(s)	940, 1149
MroI	TCCGGA	2 cut(s)	2076, 2660
MroNI	GCCGGC	1 cut(s)	328
MroXI	GAANNNNTTC	1 cut(s)	487
MseI	TTAA	9 cut(s)	266, 654, 786, 837, 867, 1253, 1536, 2184, 2207
MslI	CAYNNNNRTG	1 cut(s)	2105
MspA1I	CMGCKG	1 cut(s)	1078
MspCI	CTTAAG	1 cut(s)	2183
MspI	CCGG	7 cut(s)	287, 329, 578, 1085, 2077, 2431, 2661
MspR9I	CCNGG	5 cut(s)	287, 1085, 1488, 1899, 2432
Mva1269I	GAATGC	1 cut(s)	1276
MvaI	CCWGG	2 cut(s)	1488, 1899
MvnI	CGCG	2 cut(s)	345, 2756
MwoI	GCNNNNNNNGC	9 cut(s)	316, 325, 530, 935, 1324, 1716, 1759, 2517, 2810
NaeI	GCCGGC	1 cut(s)	330
NciI	CCSGG	3 cut(s)	287, 1085, 2432
NcoI	CCATGG	2 cut(s)	16, 2457
NdeI	CATATG	2 cut(s)	848, 2056
NgoMIV	GCCGGC	1 cut(s)	328
NlaIV	GGNNCC	6 cut(s)	277, 291, 333, 409, 2337, 2665
NsiI	ATGCAT	2 cut(s)	940, 1149
NspI	RCATGY	3 cut(s)	396, 1219, 2165
NspV	TTCGAA	1 cut(s)	2588
PaeR7I	CTCGAG	1 cut(s)	1956
PagI	TCATGA	1 cut(s)	1134
PctI	GAATGC	1 cut(s)	1276
PdiI	GCCGGC	1 cut(s)	330
PdmI	GAANNNNTTC	1 cut(s)	487
PfeI	GAWTC	6 cut(s)	545, 922, 1131, 1847, 2225, 2590
PflMI	CCANNNNNTGG	2 cut(s)	33, 157
PfoI	TCCNGGA	1 cut(s)	1897
PkrI	GCNGC	3 cut(s)	906, 1345, 2622
PleI	GAGTC	2 cut(s)	31, 2074
PpsI	GAGTC	2 cut(s)	31, 2074
Ppu21I	YACGTR	1 cut(s)	1663
PshBI	ATTAAT	1 cut(s)	786
Psp6I	CCWGG	2 cut(s)	1486, 1897
PspFI	CCCAGC	2 cut(s)	467, 1994
PspGI	CCWGG	2 cut(s)	1486, 1897
PspN4I	GGNNCC	6 cut(s)	277, 291, 333, 409, 2337, 2665
PspOMI	GGGCCC	1 cut(s)	275
PspPI	GGNCC	5 cut(s)	20, 275, 276, 1163, 1982
PstNI	CAGNNNCTG	4 cut(s)	33, 1126, 1688, 2068
PsuI	RGATCY	5 cut(s)	407, 672, 1772, 2335, 2558
PvuII	CAGCTG	1 cut(s)	1078
RsaI	GTAC	7 cut(s)	426, 854, 954, 967, 1048, 1699, 2368
RsaNI	GTAC	7 cut(s)	425, 853, 953, 966, 1047, 1698, 2367
RseI	CAYNNNNRTG	1 cut(s)	2105
SaqAI	TTAA	9 cut(s)	266, 654, 786, 837, 867, 1253, 1536, 2184, 2207
SatI	GCNGC	3 cut(s)	905, 1344, 2621
Sau96I	GGNCC	5 cut(s)	20, 275, 276, 1163, 1982
ScaI	AGTACT	1 cut(s)	2368
SchI	GAGTC	2 cut(s)	31, 2074
ScrFI	CCNGG	5 cut(s)	287, 1085, 1488, 1899, 2432
SduI	GDGCHC	3 cut(s)	279, 2364, 2815
SfaNI	GCATC	5 cut(s)	891, 938, 1290, 1554, 1740
SfcI	CTRYAG	3 cut(s)	1073, 1276, 1371
Sfr274I	CTCGAG	1 cut(s)	1956
SfuI	TTCGAA	1 cut(s)	2588
SlaI	CTCGAG	1 cut(s)	1956
SmiMI	CAYNNNNRTG	1 cut(s)	2105
SmlI	CTYRAG	3 cut(s)	1883, 1956, 2183
SmoI	CTYRAG	3 cut(s)	1883, 1956, 2183
SnaBI	TACGTA	1 cut(s)	1663
SsiI	CCGC	5 cut(s)	5, 715, 904, 1567, 2468
SspI	AATATT	3 cut(s)	100, 155, 1529
SspMI	CTAG	4 cut(s)	107, 1337, 1458, 2765
StyD4I	CCNGG	5 cut(s)	285, 1083, 1486, 1897, 2430
StyI	CCWWGG	2 cut(s)	16, 2457
TaaI	ACNGT	7 cut(s)	424, 429, 740, 1201, 1375, 2255, 2749
TaiI	ACGT	2 cut(s)	1362, 1665
TaqI	TCGA	3 cut(s)	548, 1957, 2588
TatI	WGTACW	3 cut(s)	424, 952, 2366
TauI	GCSGC	1 cut(s)	907
TfiI	GAWTC	6 cut(s)	545, 922, 1131, 1847, 2225, 2590
Tru1I	TTAA	9 cut(s)	266, 654, 786, 837, 867, 1253, 1536, 2184, 2207
Tru9I	TTAA	9 cut(s)	266, 654, 786, 837, 867, 1253, 1536, 2184, 2207
TscAI	CASTG	8 cut(s)	745, 924, 1272, 1935, 2056, 2223, 2522, 2830
TseI	GCWGC	2 cut(s)	1343, 2620
TspRI	CASTG	8 cut(s)	745, 924, 1272, 1935, 2056, 2223, 2522, 2830
Van91I	CCANNNNNTGG	2 cut(s)	33, 157
Vha464I	CTTAAG	1 cut(s)	2183
VspI	ATTAAT	1 cut(s)	786
XagI	CCTNNNNNAGG	1 cut(s)	1886
XapI	RAATTY	4 cut(s)	499, 1256, 1922, 2493
XceI	RCATGY	3 cut(s)	396, 1219, 2165
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1021, 2455
XhoI	CTCGAG	1 cut(s)	1956
XmiI	GTMKAC	1 cut(s)	1107
XmnI	GAANNNNTTC	1 cut(s)	487
XspI	CTAG	4 cut(s)	107, 1337, 1458, 2765
ZrmI	AGTACT	1 cut(s)	2368
Zsp2I	ATGCAT	2 cut(s)	940, 1149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.