MD00G1200000.v1.1

CRIB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007680
Physical Location & Seq
Reverse (-)
47296698 .. 47298298
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1200000.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGCGGCGAAAGATGCAAAGAATAGCAAGATGGTGGTGAAGGACGCAAATAATGGCAAGATGGTGGTGAAAGATGCAAATAACAACAAGATGAAGGGCCTCTTGAAGGGCCTAAGATACATTTCTCAAATTTTTGATGATAACAAAGACCAAGAAATGCAGATTGGGCTTCCTACAGATGTAAAGCATGTTGCTCATATAGGATGGGATGGCCCTTCAACTGCTAGTTCTCCAAGCTGGATGAATGAGTTTCAAGCTCCACCAGGATTTCAACAGTCAGCACCTTTAGGCAATGGAGATATGAAAGAAGATTATGGGGTCAAATGGGTCTCTGAAGATTCAAGCCAGAAAAGTTCAAGGGGTGCTAGGTCACCGGCGAATGACATGCCGGATTTACCCAAGTCGTCAAGGCGGCATTCATCGAACGGGCACGGAGGTGGTGGAACGGATTCCCCCAAGAAGGAAAAATCAGATAAAGTTAAGCATACAAGGAGGTCTTCTAAGAATAGGGAGATATCGGACAGTAGTACTAGGAGCATCCAGCAATCCACGGATCCAAGTCAAGGCAGCAAGTCGCCGCAAAACCTACCGGACATCCCTAAGAAAACGCGACGGAAGAAGTCGAAGGACTCGTCCATTGGGAGTCCATCAGGGTCAAGGAGATCTAAACACCATGCTAGTTCAGAAACATGTACATCTCCTGGACACCAATCTGCAACCATTTCTCAGAGATCTTTTCAAGAGGGGGAGGACAAGGGATTTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD

Protein Analysis

256

Amino Acids

27.74

Weight (kDa)

9.8

Isoelectric Point (pI)

80.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 610
AciI CCGC 3 cut(s) 5, 412, 578
AclWI GGATC 2 cut(s) 548, 561
AcsI RAATTY 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 2 cut(s) 529, 694
AfiI CCNNNNNNNGG 3 cut(s) 106, 460, 563
AflIII ACRYGT 1 cut(s) 689
AgsI TTSAA 7 cut(s) 106, 219, 254, 272, 342, 357, 740
AjnI CCWGG 2 cut(s) 262, 700
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AleI CACNNNNGTG 1 cut(s) 435
AluBI AGCT 2 cut(s) 237, 257
AluI AGCT 2 cut(s) 237, 257
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 2 cut(s) 548, 561
AoxI GGCC 3 cut(s) 97, 109, 211
ApeKI GCWGC 1 cut(s) 567
ApoI RAATTY 1 cut(s) 129
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
Asp700I GAANNNNTTC 1 cut(s) 448
AspS9I GGNCC 3 cut(s) 97, 109, 212
AsuHPI GGTGA 3 cut(s) 49, 79, 363
BaeGI GKGCMC 1 cut(s) 432
BamHI GGATCC 1 cut(s) 553
BbsI GAAGAC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 579
BccI CCATC 5 cut(s) 25, 55, 198, 203, 655
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 2 cut(s) 264, 702
BcoDI GTCTC 1 cut(s) 334
BfaI CTAG 4 cut(s) 225, 366, 531, 678
BfmI CTRYAG 1 cut(s) 174
BglII AGATCT 2 cut(s) 662, 731
BisI GCNGC 4 cut(s) 6, 413, 568, 578
BlsI GCNGC 4 cut(s) 7, 414, 569, 579
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 2 cut(s) 264, 702
BmgT120I GGNCC 3 cut(s) 97, 109, 212
BmiI GGNNCC 1 cut(s) 555
BmrFI CCNGG 2 cut(s) 264, 702
BmsI GCATC 3 cut(s) 4, 64, 546
BpiI GAAGAC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 1 cut(s) 549
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 460, 563
Bse118I RCCGGY 1 cut(s) 373
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 2 cut(s) 264, 702
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 5 cut(s) 209, 214, 246, 537, 594
BseLI CCNNNNNNNGG 3 cut(s) 106, 460, 563
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 740
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 610
BshFI GGCC 3 cut(s) 99, 111, 213
BsiSI CCGG 3 cut(s) 374, 389, 590
BslI CCNNNNNNNGG 3 cut(s) 106, 460, 563
BsmAI GTCTC 1 cut(s) 334
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 3 cut(s) 99, 111, 213
Bso31I GGTCTC 1 cut(s) 334
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 692
Bsp143I GATC 3 cut(s) 553, 662, 731
BspACI CCGC 3 cut(s) 5, 412, 578
BspANI GGCC 3 cut(s) 99, 111, 213
BspCNI CTCAG 1 cut(s) 739
BspFNI CGCG 1 cut(s) 610
BspLI GGNNCC 1 cut(s) 555
BspPI GGATC 2 cut(s) 548, 561
BspTNI GGTCTC 1 cut(s) 334
BsrDI GCAATG 1 cut(s) 298
BsrFI RCCGGY 1 cut(s) 373
BsrGI TGTACA 1 cut(s) 692
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 3 cut(s) 553, 662, 731
Bst2UI CCWGG 2 cut(s) 264, 702
Bst4CI ACNGT 2 cut(s) 276, 524
BstAUI TGTACA 1 cut(s) 692
BstDEI CTNAG 4 cut(s) 113, 501, 600, 726
BstDSI CCRYGG 1 cut(s) 549
BstEII GGTNACC 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 5 cut(s) 209, 214, 246, 537, 594
BstFNI CGCG 1 cut(s) 610
BstKTI GATC 3 cut(s) 556, 665, 734
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 3 cut(s) 553, 662, 731
BstMWI GCNNNNNNNGC 2 cut(s) 14, 166
BstNI CCWGG 2 cut(s) 264, 702
BstNSI RCATGY 3 cut(s) 191, 388, 693
BstPI GGTNACC 1 cut(s) 369
BstSCI CCNGG 2 cut(s) 262, 700
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 1 cut(s) 610
BstV1I GCAGC 1 cut(s) 579
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 3 cut(s) 553, 662, 731
BstYI RGATCY 3 cut(s) 553, 662, 731
BsuRI GGCC 3 cut(s) 99, 111, 213
BtgI CCRYGG 1 cut(s) 549
BtsCI GGATG 5 cut(s) 209, 214, 246, 537, 594
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 3 cut(s) 97, 109, 212
CseI GACGC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 528, 693
CviAII CATG 5 cut(s) 188, 385, 674, 690, 765
CviJI RGCY 7 cut(s) 99, 111, 169, 213, 237, 257, 345
CviKI_1 RGCY 7 cut(s) 99, 111, 169, 213, 237, 257, 345
CviQI GTAC 2 cut(s) 528, 693
DdeI CTNAG 4 cut(s) 113, 501, 600, 726
DpnI GATC 3 cut(s) 555, 664, 733
DpnII GATC 3 cut(s) 553, 662, 731
Eco31I GGTCTC 1 cut(s) 334
Eco32I GATATC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 369
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoO109I RGGNCCY 2 cut(s) 97, 109
EcoO65I GGTNACC 1 cut(s) 369
EcoRII CCWGG 2 cut(s) 262, 700
EcoRV GATATC 1 cut(s) 516
FaeI CATG 5 cut(s) 191, 388, 677, 693, 768
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 481, 513
FatI CATG 5 cut(s) 187, 384, 673, 689, 764
Fnu4HI GCNGC 4 cut(s) 6, 413, 568, 578
FokI GGATG 5 cut(s) 216, 221, 253, 524, 581
Fsp4HI GCNGC 4 cut(s) 6, 413, 568, 578
FspBI CTAG 4 cut(s) 225, 366, 531, 678
GluI GCNGC 4 cut(s) 6, 413, 568, 578
HaeIII GGCC 3 cut(s) 99, 111, 213
HapII CCGG 3 cut(s) 374, 389, 590
HgaI GACGC 1 cut(s) 53
Hin1II CATG 5 cut(s) 191, 388, 677, 693, 768
HinfI GANTC 4 cut(s) 338, 449, 629, 643
HpaII CCGG 3 cut(s) 374, 389, 590
HphI GGTGA 3 cut(s) 49, 79, 363
Hpy188I TCNGA 5 cut(s) 334, 472, 520, 685, 729
Hpy188III TCNNGA 2 cut(s) 103, 740
Hpy99I CGWCG 1 cut(s) 615
HpyAV CCTTC 6 cut(s) 34, 88, 100, 225, 454, 619
HpyCH4III ACNGT 2 cut(s) 276, 524
HpyCH4V TGCA 4 cut(s) 17, 77, 160, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 166
HpyF3I CTNAG 4 cut(s) 113, 501, 600, 726
Hsp92II CATG 5 cut(s) 191, 388, 677, 693, 768
Kzo9I GATC 3 cut(s) 553, 662, 731
LmnI GCTCC 2 cut(s) 262, 534
Lsp1109I GCAGC 1 cut(s) 579
LweI GCATC 3 cut(s) 4, 64, 546
MaeI CTAG 4 cut(s) 225, 366, 531, 678
MaeIII GTNAC 1 cut(s) 369
MalI GATC 3 cut(s) 555, 664, 733
MboI GATC 3 cut(s) 553, 662, 731
MboII GAAGA 4 cut(s) 320, 347, 489, 628
MflI RGATCY 3 cut(s) 553, 662, 731
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 1 cut(s) 129
MlyI GAGTC 2 cut(s) 623, 652
MnlI CCTC 5 cut(s) 110, 428, 486, 736, 742
MroXI GAANNNNTTC 1 cut(s) 448
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 435
MspI CCGG 3 cut(s) 374, 389, 590
MspR9I CCNGG 2 cut(s) 264, 702
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 2 cut(s) 264, 702
MvnI CGCG 1 cut(s) 610
MwoI GCNNNNNNNGC 2 cut(s) 14, 166
NdeII GATC 3 cut(s) 553, 662, 731
NlaIII CATG 5 cut(s) 191, 388, 677, 693, 768
NlaIV GGNNCC 1 cut(s) 555
NmuCI GTSAC 1 cut(s) 369
NspI RCATGY 3 cut(s) 191, 388, 693
OliI CACNNNNGTG 1 cut(s) 435
PciI ACATGT 1 cut(s) 689
PcsI WCGNNNNNNNCGW 1 cut(s) 629
PctI GAATGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 338, 449
PfoI TCCNGGA 1 cut(s) 700
PkrI GCNGC 4 cut(s) 7, 414, 569, 579
PleI GAGTC 2 cut(s) 623, 651
PpsI GAGTC 2 cut(s) 623, 651
PscI ACATGT 1 cut(s) 689
Psp6I CCWGG 2 cut(s) 262, 700
PspEI GGTNACC 1 cut(s) 369
PspGI CCWGG 2 cut(s) 262, 700
PspN4I GGNNCC 1 cut(s) 555
PspPI GGNCC 3 cut(s) 97, 109, 212
PsuI RGATCY 3 cut(s) 553, 662, 731
RsaI GTAC 2 cut(s) 529, 694
RsaNI GTAC 2 cut(s) 528, 693
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 4 cut(s) 6, 413, 568, 578
Sau3AI GATC 3 cut(s) 553, 662, 731
Sau96I GGNCC 3 cut(s) 97, 109, 212
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 2 cut(s) 623, 652
ScrFI CCNGG 2 cut(s) 264, 702
SduI GDGCHC 1 cut(s) 432
SetI ASST 7 cut(s) 239, 259, 286, 371, 439, 497, 588
SfaNI GCATC 3 cut(s) 4, 64, 546
SfcI CTRYAG 1 cut(s) 174
SgrAI CRCCGGYG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 435
Sse9I AATT 1 cut(s) 129
SsiI CCGC 3 cut(s) 5, 412, 578
SspMI CTAG 4 cut(s) 225, 366, 531, 678
StyD4I CCNGG 2 cut(s) 262, 700
TaaI ACNGT 2 cut(s) 276, 524
TaqI TCGA 2 cut(s) 422, 623
TasI AATT 1 cut(s) 129
TatI WGTACW 2 cut(s) 527, 692
TauI GCSGC 3 cut(s) 8, 415, 580
TfiI GAWTC 2 cut(s) 338, 449
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TseFI GTSAC 1 cut(s) 369
TseI GCWGC 1 cut(s) 567
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 4 cut(s) 107, 257, 317, 408
TspGWI ACGGA 4 cut(s) 447, 461, 566, 628
XagI CCTNNNNNAGG 1 cut(s) 104
XapI RAATTY 1 cut(s) 129
XceI RCATGY 3 cut(s) 191, 388, 693
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 4 cut(s) 225, 366, 531, 678
ZrmI AGTACT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.