Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0007680

Physical Location & Seq

Genomic Coordinates Forward (+)

30199716 .. 30280209

Gene Structure 80494 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0188900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGAGTTTAAGGTGCACCAGGGTCATCATCTCAAGAAAGAGCAATGGGAGAAGCTACTTATGCTACTTTGGGCTCTCTGAAAAAAATAGAAACAATAAGCTGTGGAACAATACGTGCACAAATGCTCATGCAATTGTAGTTACTACGTTCACTTGGTATGAACAATTTCTACAAGCGCAGAAGCCTTCGTTGCATGAACCAGCTGCCAAACCGAGTAAACATTGGTCTCCACCATCTAGCAATCAGCTCAAAATCAATGTTGATAGAGCTTTCTTGCCTTCTAAGGACTTTGGAGGTATTGGTGGAGTACTCAGACGAAGTGACGGCTCTTTTGTACCTGCCTTCTCAAAATTTGTGCAGCACGTCTCATCACAGGAACTGTTAGCAATTAGAGAAGGTCTAGACTTCCTAAAGCAGCGAGGCCTTCATGATGTATGCCTTGAAAGTGACTGTCAAGGAGCGATCCAAGAAATTGATACGGGGGACTGTGACTTAAATGAAAATGCAAACCTGCTGCTCGACATACAACAAATTGCCAATTTTGTGGCACATAGGTTAGCAGCCATTGCTTATGAATCCAATACTACTCAAGTATGGATAGACCAAGCTCCAGAGATGGGTTCGGTTCTAAGTTTCATAGGGATCTTATTTCTTGATGGATTCCTACTTTCCATGGCGTATAACGTTCATCACTACAGCTTTATTCTCAAAGAGACCAATTTTACACGATTGTGCACGACGAAGCCCTTGCTGACTGTAAATGGGCAGTTTCCAGGACCAACTATTCATGTTCGCAAAGGAGACACAGCTTTTGTTAATGTTCACAATGATGGCAAATACGGAATCACCCTTCATTGGCATGGAGTAAAGCAACCAAGAAATCCATGGTCAGATGGTCCTGAGAATATTACGCAGTGCCCTATTCAACCTGGAAAGAATTTTACTTATGAAGTTATATTTTCAGATGAAGAAGGAACTTTATGGTGGCATGCGCATAGCGGTTGGTCACGTGCGACGGTCCATGGTCCTATTGTTATTTTACCTCACAGGGGGAAAAGTTATCCGTTTCCCAAACCCTATGCAGAGGAAACAATTATGCTTGCATCCTGGTACTTTGGAGATGTGAAAGAACTTGTGGATGTGGCTATGGAAGAAGGTAGCGACTTGCCCCATTCAGATGCTTATACTATAAATGGTGAACCAGGAGATCTTTGTAATTGCTCTAAAGACACAACGTACCGTCGAATGGTGGACTACGGAAAGACATATCTGCTCCGTATAATCAATGCAAATATTAATGCAGAACATTTCTTTGCGGTTGGTGACCACAACCTCACTGTAGTTGGACTAGATGGAGCCTATATAAAACCCATAAACACAGGTTACCTAGTTATAAGCCCTGGACAAGCGATGGATGTCCTCCTTGAAGCAAATCAATCCCCTGGTCAATACTATATGGCTGCTAGACAATACTCGAGTGAGGATGCAGCAGTTGTGGACTTCGACCATGTCAATGTTACTGCAATCCTAGAATATAGAGGCAACTATACATATCAACCTCCTTCCTTTCCAACCACTCTTCCAATGTACTTGGACTATCCAGCAGCAGTAAAATTCACTTTACGAATTAAGAGCTTGGCGACTCCAGAATACCCTATAAGTGTCCCACTAAACGTTACTACTTCAATGTTCATAACCGTTTCCATGAATGAACTTCCTTGTACTCATTCAGGCTGTAAAACGGATCAGAAAATGACTTCAAGCTTGAATAATGTTAGCTGGGCGTTCCCACTCAATACCACAGATGTTCTCCAAGCCTACTACAGGAACATAAGCGGGGTTTACACGACAGATTTTCCAGACCAGCCAACTCAATATTATAACTTCACAGAGGAAACTTTTGTGGAAAATATTTTGTTCACTGTACAAGGGACGAAGGTGAAGGTGCTAAATTATAACGAGTCAGTTCAGATTGTGTTTCAAGGGACCGATGTGATGAAAAGCTCTGTTAATCATCCAATGCATATGCATGGATACAGTTTCTACGTGGTTGGATTTGGTTTTGGAATTTATAACAATGAGAGTGATCCAAAAGGATTTAATTTGGTTGATCCTCCTCAAGTAACTACGTTTGGAGTTCCCAAAAATGGTTGGTTAGCAATTAGATTTACAGCAAATAATCCCGGGGTATGGTTTTGGCATTGTCATTTTGAGAGACACTTGAGTTGGGGAATGGACACTGCTTTCATAGTCAAAAACGGTGGCACCGTTCAGACTAGCATGCGCGAACCCCCAGCTTATATGCCTCCATGTAATGCTCAATTGGATTCTCATATCAAAACCGATTCAACAGAAGGAGTAAATATAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PBD

Protein Analysis

790

Amino Acids

88.76

Weight (kDa)

5.95

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_3	PF13456	86 - 179	3.5e-08	Reverse transcriptase-like
Cu-oxidase_3	PF07732	237 - 349	2.7e-40	Multicopper oxidase
Cu-oxidase	PF00394	363 - 515	9.4e-38	Multicopper oxidase
Cu-oxidase_2	PF07731	621 - 753	5.8e-38	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007680)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20430 AT4G28556 AT4G28556
fragaria_vesca	FvH4_2g21080
malus_domestica	MD00G1200000.v1.1 MD05G1193500.v1.1 MD10G1180200.v1.1
prunus_persica	Prupe.8G220200_v2.0.a1 Prupe.8G220200_v2.0.a1
pyrus_communis	pycom05g18090 pycom10g15610
rosa_chinensis	RchiOBHm_Chr6g0287541
rosa_laevigata	RLG00000012486
rosa_roxburghii	Rroxscaffold_7G00180940 Rroxscaffold_7G00181390
rosa_rugosa	Rorug06G0188800 Rorug06G0188900 Rorug06G0188900
rosa_samantha	Rh6AG300100 Rh6BG305600 Rh6CG303900 Rh6CG313900 Rh6DG297300
rosa_wichuraiana	Rw6G025890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1394, 1881, 1956, 2073
Acc16I	TGCGCA	1 cut(s)	993
Acc36I	ACCTGC	2 cut(s)	346, 519
AccB1I	GGYRCC	1 cut(s)	2264
AccII	CGCG	1 cut(s)	2286
AciI	CCGC	3 cut(s)	999, 1316, 1836
AclI	AACGTT	2 cut(s)	684, 1674
AclWI	GGATC	5 cut(s)	457, 650, 1752, 2081, 2105
AcsI	RAATTY	4 cut(s)	350, 937, 1613, 2067
AcvI	CACGTG	1 cut(s)	1010
AfaI	GTAC	7 cut(s)	309, 336, 1112, 1238, 1589, 1723, 1926
AfiI	CCNNNNNNNGG	7 cut(s)	617, 855, 1049, 1246, 1824, 2147, 2190
AgsI	TTSAA	8 cut(s)	443, 926, 1427, 1686, 1761, 1768, 1982, 2349
AjiI	CACGTC	1 cut(s)	364
AjnI	CCWGG	7 cut(s)	17, 772, 928, 1106, 1201, 1399, 1441
AleI	CACNNNNGTG	1 cut(s)	730
Alw21I	GWGCWC	3 cut(s)	17, 119, 737
Alw26I	GTCTC	5 cut(s)	231, 370, 707, 795, 2209
Alw44I	GTGCAC	3 cut(s)	13, 115, 733
AlwI	GGATC	5 cut(s)	457, 650, 1752, 2081, 2105
AlwNI	CAGNNNCTG	1 cut(s)	379
Ama87I	CYCGRG	2 cut(s)	1474, 2183
AoxI	GGCC	1 cut(s)	421
ApaLI	GTGCAC	3 cut(s)	13, 115, 733
ApeKI	GCWGC	8 cut(s)	203, 358, 415, 514, 560, 1460, 1487, 1604
ApoI	RAATTY	4 cut(s)	350, 937, 1613, 2067
ArsI	GACNNNNNNTTYG	4 cut(s)	314, 346, 794, 826
AseI	ATTAAT	1 cut(s)	1296
Asp700I	GAANNNNTTC	1 cut(s)	165
AspLEI	GCGC	3 cut(s)	178, 994, 2286
AspS9I	GGNCC	5 cut(s)	776, 896, 1018, 1025, 1986
AsuC2I	CCSGG	2 cut(s)	2184, 2185
AsuHPI	GGTGA	4 cut(s)	838, 1208, 1334, 1951
AvaI	CYCGRG	2 cut(s)	1474, 2183
AvaII	GGWCC	5 cut(s)	776, 896, 1018, 1025, 1986
BaeGI	GKGCMC	4 cut(s)	17, 119, 737, 920
BanI	GGYRCC	1 cut(s)	2264
BanII	GRGCYC	1 cut(s)	75
BbrPI	CACGTG	1 cut(s)	1010
Bbv12I	GWGCWC	3 cut(s)	17, 119, 737
BbvI	GCAGC	8 cut(s)	190, 370, 427, 501, 572, 1447, 1499, 1616
BccI	CCATC	7 cut(s)	241, 610, 650, 824, 887, 1346, 1405
BceAI	ACGGC	1 cut(s)	340
BcgI	CGANNNNNNTGC	2 cut(s)	730, 764
BciT130I	CCWGG	7 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443
BciVI	GTATCC	1 cut(s)	2027
BcnI	CCSGG	2 cut(s)	2184, 2185
BcoDI	GTCTC	5 cut(s)	231, 370, 707, 795, 2209
BfaI	CTAG	7 cut(s)	237, 401, 1349, 1388, 1464, 1529, 2277
BfmI	CTRYAG	3 cut(s)	694, 1338, 1822
BfuAI	ACCTGC	2 cut(s)	346, 519
BfuI	GTATCC	1 cut(s)	2027
BglII	AGATCT	1 cut(s)	1207
BisI	GCNGC	8 cut(s)	204, 359, 416, 515, 561, 1461, 1488, 1605
BlsI	GCNGC	8 cut(s)	205, 360, 417, 516, 562, 1462, 1489, 1606
BmcAI	AGTACT	1 cut(s)	309
Bme1390I	CCNGG	9 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443, 2184, 2185
Bme18I	GGWCC	5 cut(s)	776, 896, 1018, 1025, 1986
BmeT110I	CYCGRG	2 cut(s)	1474, 2183
BmgBI	CACGTC	1 cut(s)	364
BmgT120I	GGNCC	5 cut(s)	776, 896, 1018, 1025, 1986
BmiI	GGNNCC	3 cut(s)	1357, 1987, 2266
BmrFI	CCNGG	9 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443, 2184, 2185
BmsI	GCATC	3 cut(s)	1112, 1168, 1474
BpmI	CTGGAG	2 cut(s)	594, 1629
BpuEI	CTTGAG	4 cut(s)	16, 573, 2103, 2242
BpuMI	CCSGG	2 cut(s)	2184, 2185
BsaAI	YACGTR	3 cut(s)	114, 1010, 2047
BsaI	GGTCTC	2 cut(s)	231, 707
BsaJI	CCNNGG	8 cut(s)	18, 672, 884, 1021, 1399, 1441, 2183, 2184
Bsc4I	CCNNNNNNNGG	7 cut(s)	617, 855, 1049, 1246, 1824, 2147, 2190
Bse3DI	GCAATG	2 cut(s)	49, 564
BseBI	CCWGG	7 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443
BseDI	CCNNGG	8 cut(s)	18, 672, 884, 1021, 1399, 1441, 2183, 2184
BseGI	GGATG	5 cut(s)	1103, 1144, 1420, 1489, 2014
BseLI	CCNNNNNNNGG	7 cut(s)	617, 855, 1049, 1246, 1824, 2147, 2190
BseMI	GCAATG	2 cut(s)	49, 564
BseMII	CTCAG	2 cut(s)	325, 891
BseRI	GAGGAG	1 cut(s)	2106
BseSI	GKGCMC	4 cut(s)	17, 119, 737, 920
BseXI	GCAGC	8 cut(s)	190, 370, 427, 501, 572, 1447, 1499, 1616
BseYI	CCCAGC	2 cut(s)	1779, 2293
BsgI	GTGCAG	1 cut(s)	377
Bsh1236I	CGCG	1 cut(s)	2286
BshFI	GGCC	1 cut(s)	423
BshNI	GGYRCC	1 cut(s)	2264
BsiHKAI	GWGCWC	3 cut(s)	17, 119, 737
BsiHKCI	CYCGRG	2 cut(s)	1474, 2183
BsiSI	CCGG	1 cut(s)	2184
BslFI	GGGAC	4 cut(s)	497, 1649, 1945, 1999
BslI	CCNNNNNNNGG	7 cut(s)	617, 855, 1049, 1246, 1824, 2147, 2190
BsmAI	GTCTC	5 cut(s)	231, 370, 707, 795, 2209
BsmBI	CGTCTC	1 cut(s)	370
BsmFI	GGGAC	4 cut(s)	497, 1649, 1945, 1999
BsnI	GGCC	1 cut(s)	423
Bso31I	GGTCTC	2 cut(s)	231, 707
BsoBI	CYCGRG	2 cut(s)	1474, 2183
Bsp1286I	GDGCHC	5 cut(s)	17, 75, 119, 737, 920
Bsp1407I	TGTACA	1 cut(s)	1924
Bsp143I	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
Bsp19I	CCATGG	3 cut(s)	672, 884, 1021
BspACI	CCGC	3 cut(s)	999, 1316, 1836
BspANI	GGCC	1 cut(s)	423
BspCNI	CTCAG	2 cut(s)	324, 892
BspFNI	CGCG	1 cut(s)	2286
BspHI	TCATGA	1 cut(s)	427
BspLI	GGNNCC	3 cut(s)	1357, 1987, 2266
BspMI	ACCTGC	2 cut(s)	346, 519
BspPI	GGATC	5 cut(s)	457, 650, 1752, 2081, 2105
BspT107I	GGYRCC	1 cut(s)	2264
BspTNI	GGTCTC	2 cut(s)	231, 707
BsrDI	GCAATG	2 cut(s)	49, 564
BsrGI	TGTACA	1 cut(s)	1924
BssECI	CCNNGG	8 cut(s)	18, 672, 884, 1021, 1399, 1441, 2183, 2184
BssMI	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
BssT1I	CCWWGG	3 cut(s)	672, 884, 1021
Bst2UI	CCWGG	7 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443
Bst6I	CTCTTC	1 cut(s)	1584
BstAPI	GCANNNNNTGC	1 cut(s)	566
BstAUI	TGTACA	1 cut(s)	1924
BstBAI	YACGTR	3 cut(s)	114, 1010, 2047
BstC8I	GCNNGC	3 cut(s)	990, 1101, 2282
BstDEI	CTNAG	4 cut(s)	282, 311, 629, 900
BstDSI	CCRYGG	3 cut(s)	672, 884, 1021
BstEII	GGTNACC	2 cut(s)	1322, 1382
BstENI	CCTNNNNNAGG	1 cut(s)	1822
BstF5I	GGATG	5 cut(s)	1103, 1144, 1420, 1489, 2014
BstFNI	CGCG	1 cut(s)	2286
BstHHI	GCGC	3 cut(s)	178, 994, 2286
BstKTI	GATC	6 cut(s)	465, 645, 1210, 1747, 2089, 2113
BstMAI	GTCTC	5 cut(s)	231, 370, 707, 795, 2209
BstMBI	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
BstMWI	GCNNNNNNNGC	3 cut(s)	60, 190, 566
BstNI	CCWGG	7 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443
BstNSI	RCATGY	2 cut(s)	992, 2284
BstPI	GGTNACC	2 cut(s)	1322, 1382
BstSCI	CCNGG	9 cut(s)	17, 772, 928, 1106, 1201, 1399, 1441, 2182, 2183
BstSFI	CTRYAG	3 cut(s)	694, 1338, 1822
BstSLI	GKGCMC	4 cut(s)	17, 119, 737, 920
BstUI	CGCG	1 cut(s)	2286
BstV1I	GCAGC	8 cut(s)	190, 370, 427, 501, 572, 1447, 1499, 1616
BstX2I	RGATCY	2 cut(s)	642, 1207
BstXI	CCANNNNNNTGG	1 cut(s)	544
BstYI	RGATCY	2 cut(s)	642, 1207
BsuI	GTATCC	1 cut(s)	2027
BsuRI	GGCC	1 cut(s)	423
BtgI	CCRYGG	3 cut(s)	672, 884, 1021
BtgZI	GCGATG	1 cut(s)	1424
BtrI	CACGTC	1 cut(s)	364
BtsCI	GGATG	5 cut(s)	1103, 1144, 1420, 1489, 2014
BtsI	GCAGTG	2 cut(s)	920, 2238
BtsIMutI	CAGTG	4 cut(s)	920, 1335, 1920, 2238
BveI	ACCTGC	2 cut(s)	346, 519
Cac8I	GCNNGC	3 cut(s)	990, 1101, 2282
CaiI	CAGNNNCTG	1 cut(s)	379
CciI	TCATGA	1 cut(s)	427
CfoI	GCGC	3 cut(s)	178, 994, 2286
Cfr13I	GGNCC	5 cut(s)	776, 896, 1018, 1025, 1986
Cfr9I	CCCGGG	1 cut(s)	2183
Csp6I	GTAC	7 cut(s)	308, 335, 1111, 1237, 1588, 1722, 1925
CspCI	CAANNNNNGTGG	2 cut(s)	2242, 2277
CviQI	GTAC	7 cut(s)	308, 335, 1111, 1237, 1588, 1722, 1925
DdeI	CTNAG	4 cut(s)	282, 311, 629, 900
DpnI	GATC	6 cut(s)	464, 644, 1209, 1746, 2088, 2112
DpnII	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
Eam1104I	CTCTTC	1 cut(s)	1584
EarI	CTCTTC	1 cut(s)	1584
Eco130I	CCWWGG	3 cut(s)	672, 884, 1021
Eco147I	AGGCCT	1 cut(s)	423
Eco24I	GRGCYC	1 cut(s)	75
Eco31I	GGTCTC	2 cut(s)	231, 707
Eco47I	GGWCC	5 cut(s)	776, 896, 1018, 1025, 1986
Eco72I	CACGTG	1 cut(s)	1010
Eco88I	CYCGRG	2 cut(s)	1474, 2183
Eco91I	GGTNACC	2 cut(s)	1322, 1382
EcoNI	CCTNNNNNAGG	1 cut(s)	1822
EcoO65I	GGTNACC	2 cut(s)	1322, 1382
EcoRII	CCWGG	7 cut(s)	17, 772, 928, 1106, 1201, 1399, 1441
EcoT14I	CCWWGG	3 cut(s)	672, 884, 1021
EcoT22I	ATGCAT	2 cut(s)	2025, 2031
EcoT38I	GRGCYC	1 cut(s)	75
ErhI	CCWWGG	3 cut(s)	672, 884, 1021
Esp3I	CGTCTC	1 cut(s)	370
FaqI	GGGAC	4 cut(s)	497, 1649, 1945, 1999
FauI	CCCGC	1 cut(s)	1829
FauNDI	CATATG	1 cut(s)	2025
Fnu4HI	GCNGC	8 cut(s)	204, 359, 416, 515, 561, 1461, 1488, 1605
FokI	GGATG	5 cut(s)	1090, 1151, 1427, 1496, 2001
FriOI	GRGCYC	1 cut(s)	75
Fsp4HI	GCNGC	8 cut(s)	204, 359, 416, 515, 561, 1461, 1488, 1605
FspAI	RTGCGCAY	1 cut(s)	993
FspBI	CTAG	7 cut(s)	237, 401, 1349, 1388, 1464, 1529, 2277
FspI	TGCGCA	1 cut(s)	993
GlaI	GCGC	3 cut(s)	177, 993, 2285
GluI	GCNGC	8 cut(s)	204, 359, 416, 515, 561, 1461, 1488, 1605
GsaI	CCCAGC	2 cut(s)	1783, 2297
GsuI	CTGGAG	2 cut(s)	594, 1629
HaeIII	GGCC	1 cut(s)	423
HapII	CCGG	1 cut(s)	2184
HhaI	GCGC	3 cut(s)	178, 994, 2286
Hin6I	GCGC	3 cut(s)	176, 992, 2284
HinP1I	GCGC	3 cut(s)	176, 992, 2284
HindIII	AAGCTT	1 cut(s)	1762
HinfI	GANTC	7 cut(s)	575, 660, 843, 1642, 1961, 2327, 2345
HpaII	CCGG	1 cut(s)	2184
HphI	GGTGA	4 cut(s)	838, 1208, 1334, 1951
Hpy188I	TCNGA	9 cut(s)	79, 314, 892, 964, 1177, 1749, 1971, 2274, 2372
Hpy188III	TCNNGA	8 cut(s)	33, 401, 428, 611, 653, 899, 1646, 1859
Hpy99I	CGWCG	3 cut(s)	742, 1018, 1245
HpyCH4IV	ACGT	9 cut(s)	113, 146, 363, 684, 1009, 1235, 1674, 2046, 2129
HpyF10VI	GCNNNNNNNGC	3 cut(s)	60, 190, 566
HpyF3I	CTNAG	4 cut(s)	282, 311, 629, 900
HpySE526I	ACGT	9 cut(s)	113, 146, 363, 684, 1009, 1235, 1674, 2046, 2129
HspAI	GCGC	3 cut(s)	176, 992, 2284
Kzo9I	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
LmnI	GCTCC	4 cut(s)	458, 613, 1278, 1355
Lsp1109I	GCAGC	8 cut(s)	190, 370, 427, 501, 572, 1447, 1499, 1616
LweI	GCATC	3 cut(s)	1112, 1168, 1474
MaeI	CTAG	7 cut(s)	237, 401, 1349, 1388, 1464, 1529, 2277
MaeII	ACGT	9 cut(s)	113, 146, 363, 684, 1009, 1235, 1674, 2046, 2129
MalI	GATC	6 cut(s)	464, 644, 1209, 1746, 2088, 2112
MboI	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
MboII	GAAGA	3 cut(s)	980, 1163, 1571
MfeI	CAATTG	2 cut(s)	132, 2321
MflI	RGATCY	2 cut(s)	642, 1207
MhlI	GDGCHC	5 cut(s)	17, 75, 119, 737, 920
MlyI	GAGTC	2 cut(s)	1636, 1970
MmeI	TCCRAC	3 cut(s)	1324, 1595, 2032
Mph1103I	ATGCAT	2 cut(s)	2025, 2031
MroXI	GAANNNNTTC	1 cut(s)	165
MseI	TTAA	7 cut(s)	8, 494, 816, 1296, 1629, 2010, 2100
MslI	CAYNNNNRTG	6 cut(s)	730, 828, 858, 1176, 1512, 2028
MspA1I	CMGCKG	1 cut(s)	203
MspI	CCGG	1 cut(s)	2184
MspR9I	CCNGG	9 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443, 2184, 2185
MunI	CAATTG	2 cut(s)	132, 2321
MvaI	CCWGG	7 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443
MvnI	CGCG	1 cut(s)	2286
MwoI	GCNNNNNNNGC	3 cut(s)	60, 190, 566
NciI	CCSGG	2 cut(s)	2184, 2185
NcoI	CCATGG	3 cut(s)	672, 884, 1021
NdeI	CATATG	1 cut(s)	2025
NdeII	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
NlaIV	GGNNCC	3 cut(s)	1357, 1987, 2266
NmuCI	GTSAC	5 cut(s)	320, 446, 488, 1005, 1322
NsbI	TGCGCA	1 cut(s)	993
NsiI	ATGCAT	2 cut(s)	2025, 2031
NspI	RCATGY	2 cut(s)	992, 2284
OliI	CACNNNNGTG	1 cut(s)	730
PaeI	GCATGC	2 cut(s)	992, 2284
PaeR7I	CTCGAG	1 cut(s)	1474
PagI	TCATGA	1 cut(s)	427
PceI	AGGCCT	1 cut(s)	423
PcsI	WCGNNNNNNNCGW	1 cut(s)	2265
PdmI	GAANNNNTTC	1 cut(s)	165
PfeI	GAWTC	5 cut(s)	575, 660, 843, 2327, 2345
PflFI	GACNNNGTC	1 cut(s)	1508
PfoI	TCCNGGA	1 cut(s)	772
PkrI	GCNGC	8 cut(s)	205, 360, 417, 516, 562, 1462, 1489, 1606
PleI	GAGTC	2 cut(s)	1636, 1969
PmaCI	CACGTG	1 cut(s)	1010
PmlI	CACGTG	1 cut(s)	1010
PpsI	GAGTC	2 cut(s)	1636, 1969
Ppu21I	YACGTR	3 cut(s)	114, 1010, 2047
PshBI	ATTAAT	1 cut(s)	1296
PsiI	TTATAA	4 cut(s)	1394, 1881, 1956, 2073
Psp1406I	AACGTT	2 cut(s)	684, 1674
Psp6I	CCWGG	7 cut(s)	17, 772, 928, 1106, 1201, 1399, 1441
PspCI	CACGTG	1 cut(s)	1010
PspEI	GGTNACC	2 cut(s)	1322, 1382
PspFI	CCCAGC	2 cut(s)	1779, 2293
PspGI	CCWGG	7 cut(s)	17, 772, 928, 1106, 1201, 1399, 1441
PspN4I	GGNNCC	3 cut(s)	1357, 1987, 2266
PspPI	GGNCC	5 cut(s)	776, 896, 1018, 1025, 1986
PspXI	VCTCGAGB	1 cut(s)	1474
PsrI	GAACNNNNNNTAC	2 cut(s)	153, 185
PstNI	CAGNNNCTG	1 cut(s)	379
PsuI	RGATCY	2 cut(s)	642, 1207
PsyI	GACNNNGTC	1 cut(s)	1508
PvuII	CAGCTG	1 cut(s)	203
RsaI	GTAC	7 cut(s)	309, 336, 1112, 1238, 1589, 1723, 1926
RsaNI	GTAC	7 cut(s)	308, 335, 1111, 1237, 1588, 1722, 1925
RseI	CAYNNNNRTG	6 cut(s)	730, 828, 858, 1176, 1512, 2028
SaqAI	TTAA	7 cut(s)	8, 494, 816, 1296, 1629, 2010, 2100
SatI	GCNGC	8 cut(s)	204, 359, 416, 515, 561, 1461, 1488, 1605
Sau3AI	GATC	6 cut(s)	462, 642, 1207, 1744, 2086, 2110
Sau96I	GGNCC	5 cut(s)	776, 896, 1018, 1025, 1986
ScaI	AGTACT	1 cut(s)	309
SchI	GAGTC	2 cut(s)	1636, 1970
ScrFI	CCNGG	9 cut(s)	19, 774, 930, 1108, 1203, 1401, 1443, 2184, 2185
SduI	GDGCHC	5 cut(s)	17, 75, 119, 737, 920
SfaNI	GCATC	3 cut(s)	1112, 1168, 1474
SfcI	CTRYAG	3 cut(s)	694, 1338, 1822
Sfr274I	CTCGAG	1 cut(s)	1474
SinI	GGWCC	5 cut(s)	776, 896, 1018, 1025, 1986
SlaI	CTCGAG	1 cut(s)	1474
SmaI	CCCGGG	1 cut(s)	2185
SmiMI	CAYNNNNRTG	6 cut(s)	730, 828, 858, 1176, 1512, 2028
SmlI	CTYRAG	5 cut(s)	31, 588, 1474, 2118, 2221
SmoI	CTYRAG	5 cut(s)	31, 588, 1474, 2118, 2221
SphI	GCATGC	2 cut(s)	992, 2284
SseBI	AGGCCT	1 cut(s)	423
SsiI	CCGC	3 cut(s)	999, 1316, 1836
SspI	AATATT	4 cut(s)	907, 1294, 1877, 1912
SspMI	CTAG	7 cut(s)	237, 401, 1349, 1388, 1464, 1529, 2277
StuI	AGGCCT	1 cut(s)	423
StyD4I	CCNGG	9 cut(s)	17, 772, 928, 1106, 1201, 1399, 1441, 2182, 2183
StyI	CCWWGG	3 cut(s)	672, 884, 1021
TaiI	ACGT	9 cut(s)	116, 149, 366, 687, 1012, 1238, 1677, 2049, 2132
TaqI	TCGA	4 cut(s)	519, 1243, 1475, 1503
TaqII	GACCGA	1 cut(s)	2003
TatI	WGTACW	4 cut(s)	307, 1587, 1721, 1924
TfiI	GAWTC	5 cut(s)	575, 660, 843, 2327, 2345
Tru1I	TTAA	7 cut(s)	8, 494, 816, 1296, 1629, 2010, 2100
Tru9I	TTAA	7 cut(s)	8, 494, 816, 1296, 1629, 2010, 2100
TscAI	CASTG	4 cut(s)	920, 1342, 1927, 2245
TseFI	GTSAC	5 cut(s)	320, 446, 488, 1005, 1322
TseI	GCWGC	8 cut(s)	203, 358, 415, 514, 560, 1460, 1487, 1604
Tsp45I	GTSAC	5 cut(s)	320, 446, 488, 1005, 1322
TspGWI	ACGGA	5 cut(s)	855, 1053, 1265, 1272, 1757
TspMI	CCCGGG	1 cut(s)	2183
TspRI	CASTG	4 cut(s)	920, 1342, 1927, 2245
Tth111I	GACNNNGTC	1 cut(s)	1508
VneI	GTGCAC	3 cut(s)	13, 115, 733
VpaK11BI	GGWCC	5 cut(s)	776, 896, 1018, 1025, 1986
VspI	ATTAAT	1 cut(s)	1296
XagI	CCTNNNNNAGG	1 cut(s)	1822
XapI	RAATTY	4 cut(s)	350, 937, 1613, 2067
XbaI	TCTAGA	1 cut(s)	400
XceI	RCATGY	2 cut(s)	992, 2284
XcmI	CCANNNNNNNNNTGG	1 cut(s)	882
XhoI	CTCGAG	1 cut(s)	1474
XmaI	CCCGGG	1 cut(s)	2183
XmnI	GAANNNNTTC	1 cut(s)	165
XspI	CTAG	7 cut(s)	237, 401, 1349, 1388, 1464, 1529, 2277
ZrmI	AGTACT	1 cut(s)	309
Zsp2I	ATGCAT	2 cut(s)	2025, 2031

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.