pycom05g18090

CRIB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007680
Physical Location & Seq
Reverse (-)
21116977 .. 21118105
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18090.1

Sequence Viewer

Length: 789 bp
ATGGCGGCGAAAGATGCAAAGAATAGCAAGATGGTGGCGAAGGATGCAAATAATGGCAAGATGGTGATGAAAGATGCAAATAACAACAAGATGAAGGGCCTCTTGAAGGGCCTAAGATACATTTCTCAAATTTTTGAAATGGCATTCCTTTCGGCAGATGATAACAAAAACCAAGAAATGCAGATTGGGCTTCCTACAGATGTAAAGCATGTGGCTCATATAGGATGGGATGGCCCTTCGACTGCTAGTTCTCCAAGCTGGATGAATGAGTTTCAAGCTCCACCAGGATTTCAACAGTCAGCACCTTTAGGCAATGGAGAGATGAAAGAAGATTATGGGGTCAAATGGGTCTCTGAAGATTCAAGCCAGAAAGGTTCAAGGGGTGCTAGGTCACCGGCACATGACATGCCGGATTTACCCAAGTCGTCAAGGCGGCATTCATCGAACGGCCACGGAGGTGGTGGAACGGATTCCCCCAAGAAGGAGAAATCAGATAAAGTTAAGCATACAAGGAGGTCTTCTAAAAATAGGGAGACATCGGATATTAGTACTAGGGGCATCCAGCAACCCACGGATCCAAGTCAAGGCAGCGAGTCGCCGCAAAACCGACCTGACATCCCTAAGAAAACGCGACGGAAGAAGTCGAAGGACTCGTCCATTGGGAGTTCATCAGGGTCAAGGAGATCTAAACACCATGCTAGTTCAGAAACATGTCCATCTCCTGGACACCAATCTGCAACCATTTCTCAGAGATCTTTTCAAGAGGGGGAGGACAAGGGATTTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD

Protein Analysis

263

Amino Acids

28.52

Weight (kDa)

9.77

Isoelectric Point (pI)

72.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 722
AccII CGCG 1 cut(s) 631
AciI CCGC 3 cut(s) 5, 433, 599
AclWI GGATC 2 cut(s) 569, 582
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 4 cut(s) 106, 481, 584, 722
AflIII ACRYGT 1 cut(s) 710
AgsI TTSAA 7 cut(s) 106, 137, 275, 293, 363, 378, 761
AjnI CCWGG 2 cut(s) 283, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AleI CACNNNNGTG 1 cut(s) 456
AluBI AGCT 2 cut(s) 258, 278
AluI AGCT 2 cut(s) 258, 278
Alw26I GTCTC 2 cut(s) 355, 527
AlwI GGATC 2 cut(s) 569, 582
AoxI GGCC 4 cut(s) 97, 109, 232, 448
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 1 cut(s) 129
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
Asp700I GAANNNNTTC 1 cut(s) 469
AspS9I GGNCC 3 cut(s) 97, 109, 233
AsuHPI GGTGA 2 cut(s) 76, 384
BamHI GGATCC 1 cut(s) 574
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 600
BccI CCATC 5 cut(s) 25, 55, 219, 224, 724
BceAI ACGGC 1 cut(s) 463
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BciT130I CCWGG 2 cut(s) 285, 723
BcoDI GTCTC 2 cut(s) 355, 527
BfaI CTAG 4 cut(s) 246, 387, 552, 699
BfmI CTRYAG 1 cut(s) 195
BglII AGATCT 2 cut(s) 683, 752
BisI GCNGC 4 cut(s) 6, 434, 589, 599
BlsI GCNGC 4 cut(s) 7, 435, 590, 600
BmcAI AGTACT 1 cut(s) 550
Bme1390I CCNGG 2 cut(s) 285, 723
BmgT120I GGNCC 3 cut(s) 97, 109, 233
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 2 cut(s) 285, 723
BmsI GCATC 4 cut(s) 4, 34, 64, 567
BpiI GAAGAC 1 cut(s) 510
BsaI GGTCTC 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 451, 570
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 481, 584, 722
Bse118I RCCGGY 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 2 cut(s) 285, 723
BseDI CCNNGG 2 cut(s) 451, 570
BseGI GGATG 6 cut(s) 49, 230, 235, 267, 558, 615
BseLI CCNNNNNNNGG 4 cut(s) 106, 481, 584, 722
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 761
BseXI GCAGC 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 631
BshFI GGCC 4 cut(s) 99, 111, 234, 450
BsiSI CCGG 2 cut(s) 395, 410
BslI CCNNNNNNNGG 4 cut(s) 106, 481, 584, 722
BsmAI GTCTC 2 cut(s) 355, 527
BsmI GAATGC 2 cut(s) 143, 436
BsnI GGCC 4 cut(s) 99, 111, 234, 450
Bso31I GGTCTC 1 cut(s) 355
Bsp143I GATC 3 cut(s) 574, 683, 752
BspACI CCGC 3 cut(s) 5, 433, 599
BspANI GGCC 4 cut(s) 99, 111, 234, 450
BspCNI CTCAG 1 cut(s) 760
BspFNI CGCG 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 576
BspPI GGATC 2 cut(s) 569, 582
BspTNI GGTCTC 1 cut(s) 355
BsrDI GCAATG 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 394
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 2 cut(s) 451, 570
BssMI GATC 3 cut(s) 574, 683, 752
Bst2UI CCWGG 2 cut(s) 285, 723
Bst4CI ACNGT 1 cut(s) 297
BstDEI CTNAG 3 cut(s) 113, 621, 747
BstDSI CCRYGG 2 cut(s) 451, 570
BstEII GGTNACC 1 cut(s) 390
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 6 cut(s) 49, 230, 235, 267, 558, 615
BstFNI CGCG 1 cut(s) 631
BstKTI GATC 3 cut(s) 577, 686, 755
BstMAI GTCTC 2 cut(s) 355, 527
BstMBI GATC 3 cut(s) 574, 683, 752
BstMWI GCNNNNNNNGC 3 cut(s) 14, 44, 187
BstNI CCWGG 2 cut(s) 285, 723
BstNSI RCATGY 3 cut(s) 212, 409, 714
BstPI GGTNACC 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 283, 721
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 1 cut(s) 600
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 3 cut(s) 574, 683, 752
BstXI CCANNNNNNTGG 1 cut(s) 458
BstYI RGATCY 3 cut(s) 574, 683, 752
BsuRI GGCC 4 cut(s) 99, 111, 234, 450
BtgI CCRYGG 2 cut(s) 451, 570
BtsCI GGATG 6 cut(s) 49, 230, 235, 267, 558, 615
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 3 cut(s) 97, 109, 233
Csp6I GTAC 1 cut(s) 549
CviAII CATG 6 cut(s) 209, 401, 406, 695, 711, 786
CviJI RGCY 9 cut(s) 99, 111, 190, 215, 234, 258, 278, 366, 450
CviKI_1 RGCY 9 cut(s) 99, 111, 190, 215, 234, 258, 278, 366, 450
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 3 cut(s) 113, 621, 747
DpnI GATC 3 cut(s) 576, 685, 754
DpnII GATC 3 cut(s) 574, 683, 752
EaeI YGGCCR 1 cut(s) 448
Eco31I GGTCTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 375
Eco91I GGTNACC 1 cut(s) 390
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoO109I RGGNCCY 2 cut(s) 97, 109
EcoO65I GGTNACC 1 cut(s) 390
EcoRII CCWGG 2 cut(s) 283, 721
FaeI CATG 6 cut(s) 212, 404, 409, 698, 714, 789
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 502, 534
FatI CATG 6 cut(s) 208, 400, 405, 694, 710, 785
Fnu4HI GCNGC 4 cut(s) 6, 434, 589, 599
FokI GGATG 6 cut(s) 56, 237, 242, 274, 545, 602
Fsp4HI GCNGC 4 cut(s) 6, 434, 589, 599
FspBI CTAG 4 cut(s) 246, 387, 552, 699
GluI GCNGC 4 cut(s) 6, 434, 589, 599
HaeIII GGCC 4 cut(s) 99, 111, 234, 450
HapII CCGG 2 cut(s) 395, 410
Hin1II CATG 6 cut(s) 212, 404, 409, 698, 714, 789
HinfI GANTC 4 cut(s) 359, 470, 593, 650
HpaII CCGG 2 cut(s) 395, 410
HphI GGTGA 2 cut(s) 76, 384
Hpy188I TCNGA 5 cut(s) 355, 493, 541, 706, 750
Hpy188III TCNNGA 2 cut(s) 103, 761
Hpy99I CGWCG 1 cut(s) 636
HpyAV CCTTC 6 cut(s) 34, 88, 100, 246, 475, 640
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4V TGCA 5 cut(s) 17, 47, 77, 181, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 44, 187
HpyF3I CTNAG 3 cut(s) 113, 621, 747
Hsp92II CATG 6 cut(s) 212, 404, 409, 698, 714, 789
Kzo9I GATC 3 cut(s) 574, 683, 752
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 1 cut(s) 600
LweI GCATC 4 cut(s) 4, 34, 64, 567
MaeI CTAG 4 cut(s) 246, 387, 552, 699
MaeIII GTNAC 1 cut(s) 390
MalI GATC 3 cut(s) 576, 685, 754
MboI GATC 3 cut(s) 574, 683, 752
MboII GAAGA 4 cut(s) 341, 368, 510, 649
MflI RGATCY 3 cut(s) 574, 683, 752
MluCI AATT 1 cut(s) 129
MlyI GAGTC 2 cut(s) 602, 644
MnlI CCTC 5 cut(s) 110, 449, 507, 757, 763
MroXI GAANNNNTTC 1 cut(s) 469
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 1 cut(s) 456
MspI CCGG 2 cut(s) 395, 410
MspR9I CCNGG 2 cut(s) 285, 723
Mva1269I GAATGC 2 cut(s) 143, 436
MvaI CCWGG 2 cut(s) 285, 723
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 3 cut(s) 14, 44, 187
NdeII GATC 3 cut(s) 574, 683, 752
NlaIII CATG 6 cut(s) 212, 404, 409, 698, 714, 789
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 1 cut(s) 390
NspI RCATGY 3 cut(s) 212, 409, 714
OliI CACNNNNGTG 1 cut(s) 456
PciI ACATGT 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 650
PctI GAATGC 2 cut(s) 143, 436
PdmI GAANNNNTTC 1 cut(s) 469
PfeI GAWTC 2 cut(s) 359, 470
PflMI CCANNNNNTGG 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 721
PkrI GCNGC 4 cut(s) 7, 435, 590, 600
PleI GAGTC 2 cut(s) 601, 644
PpsI GAGTC 2 cut(s) 601, 644
PscI ACATGT 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 283, 721
PspEI GGTNACC 1 cut(s) 390
PspGI CCWGG 2 cut(s) 283, 721
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 3 cut(s) 97, 109, 233
PsuI RGATCY 3 cut(s) 574, 683, 752
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
RseI CAYNNNNRTG 1 cut(s) 456
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 4 cut(s) 6, 434, 589, 599
Sau3AI GATC 3 cut(s) 574, 683, 752
Sau96I GGNCC 3 cut(s) 97, 109, 233
ScaI AGTACT 1 cut(s) 550
SchI GAGTC 2 cut(s) 602, 644
ScrFI CCNGG 2 cut(s) 285, 723
SetI ASST 8 cut(s) 260, 280, 307, 376, 392, 460, 518, 613
SfaNI GCATC 4 cut(s) 4, 34, 64, 567
SfcI CTRYAG 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 456
Sse9I AATT 1 cut(s) 129
SsiI CCGC 3 cut(s) 5, 433, 599
SspMI CTAG 4 cut(s) 246, 387, 552, 699
StyD4I CCNGG 2 cut(s) 283, 721
TaaI ACNGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 239, 443, 644
TasI AATT 1 cut(s) 129
TatI WGTACW 1 cut(s) 548
TauI GCSGC 3 cut(s) 8, 436, 601
TfiI GAWTC 2 cut(s) 359, 470
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TseFI GTSAC 1 cut(s) 390
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 1 cut(s) 390
TspDTI ATGAA 6 cut(s) 83, 107, 278, 338, 429, 657
TspGWI ACGGA 4 cut(s) 468, 482, 587, 649
Van91I CCANNNNNTGG 1 cut(s) 722
XagI CCTNNNNNAGG 1 cut(s) 104
XapI RAATTY 1 cut(s) 129
XceI RCATGY 3 cut(s) 212, 409, 714
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 469
XspI CTAG 4 cut(s) 246, 387, 552, 699
ZrmI AGTACT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.