Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005483

Physical Location & Seq

Genomic Coordinates Forward (+)

13779765 .. 13784151

Gene Structure 4387 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1040900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGCTCTACTTGGGGACGACGGTCGTGGTTACGAACTCGCCTGCCAACTCGAATCCTGCAACGTCTGGCGCACATGGCTCGGCGATTCCACCTACGCCATCTTCGCCCCCTTCCTCATTTCCCCTTCAACCTGGGAAGCTTTCATGGACTCCAAATCTAGGCCTCACCTCCATCTCCAACTCCGCGCTCGAGCTCTTCTTTTCGATAAGGCCTGCGTTTCCCTCTTCCTCCACCCCAATTCCTCCACCTCCTCCTCTTCTTCTTCAATCGCCGTTTCCAAGCTCAATCCCAATTATTTGCAGCTCCACCCGGACGACGTCTACTTCACTCTAGATAATTCGTCTCAGGATGGGGTTCAAGTTCAACAGCGTGACGCTTCGGTGCCGTCCAAGGTTCAATCTAAAGCAGCTTTTGGTGCTGGATCAAGATATGGAGAATCTGAAATTGATAACATATCATTGAGATTCAAAAATGGAGAGCTGCCTGAAACATGGTATAATCAGTTTATTGAGAGATATAGAATGAGCAAACCCTATAGGTTGTCTTCGGTGGAGCGAGAATCAGAGAAACGTACCCCAGAGGAGATGTCTGCTTACCTTAAGCTTCTTGAGAGTCATAAGAAAAGACGAGTAGCTTTCAAAGAAAATCCAAATATGGGATATGGAAATCCCATCCAAGATGCATCACATATGAATCCAAGTTCTCTTTTAGATGGTTCAGTTGATAGTGAAACATCTTTTTTCCCGGAAACAATGTTTACATTCAACTGTGTTCCTGATAGTGCACTTCCACCTTTGAATAGAGTGGGTGACAACAAAAAAGTGGAATGTTATGGAGTTCTTGATACATTGCCTCAGATAATGACTAGGAGTCCCGTTATGCTTGAGAGGCTTGGCATCAGGCCTGAATACCTCAGCATGGAACAAGGAGGAATCTTACATCGCGGAAAAAATGCTTCTGGAGGGAACAAAAAATGTCTTAGTCAAGAGCAAGCAACACAGTTATCTCAAAAGGTAGTAGCTAGGATGATGACAAATATTGGGTTTGAAGGTACTACAGAAGTTCCAATTGATGTTTTCTCTCAGTTACTAAGCTGCCATATCCTGAAGTTGGGTGGCAGCTTGAAACTTCTTGCTGATAGTTACAGAAAGCAGTGCTCAGCCCTTGAACTGCTAAAGATGTTCCTTCAAACTGTGGGATATGGTAATTTTGGTGCTCTGGTGGAGCAAGTCAAAGATGGCTCCAAGAATTTTCAGCAAACTCAGCAGCAAATTCACGGAATCCAGTCTCAGTTGCAGCCACAGCACCAGAATCTTATTCGACTACCCCAACAAGCCCGACAAATGCATCCACAGGTGCAACAAATTGCCCATTCCAAAAACGTGCCTTTACAGCAGCAGCAGCAGTTAGAAAGAATGCGAAGACGTCAACCATCCACTCCTCGCACGGGTATGGATATGGAGAAGGATAGACCAATGGTACAGGTCAAGATTGAAGCCCCATCAGAGTTACCCATGGATGGTAATGCCTTCAACAGTTTCAATAGCAGACATCCACAGATGCAGTTCAGGCAGCAGCAAATTCCTCCAATGTCGAATCTTACAATGTCAAATGTCCACGCTCAGTCGGGCAATCAATTTAGACAGATGGCTTCCCAAATTCCTCAAGTCCAAGCACAGAACATGAGTGTTCTGAGGGCTCCACCCGTGAAGGTGGAAGGATTCCAGGAATTGATGGGCGGAGATACTTCATCAAAGCATGACTCCGATGAAAATAGATTGACCTCTCCCTCAAGTAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bromo_TP

Protein Analysis

601

Amino Acids

67.57

Weight (kDa)

8.73

Isoelectric Point (pI)

58.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65540
fragaria_vesca	FvH4_1g19490 FvH4_1g19490
malus_domestica	MD01G1040900.v1.1 MD15G1310400.v1.1
prunus_persica	Prupe.6G203300_v2.0.a1
pyrus_communis	pycom01g06850 pycom15g27420
rosa_chinensis	RchiOBHm_Chr2g0110871 RchiOBHm_Chr2g0110941
rosa_laevigata	RLG00000017847 RLG00000017849 RLG00000017853 RLG00000017855
rosa_multiflora	Rmu_sc0003994.1_g000015 Rmu_sc0003994.1_g000016 Rmu_sc0032518.1_g000001 Rmu_sc0032519.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132780 Rroxscaffold_2G00132790
rosa_rugosa	Rorug02G0170300 Rorug02G0170300
rosa_samantha	Rh2AG222300 Rh2BG233100 Rh2BG233800 Rh2CG225100 Rh2CG225400 Rh2DG230300
rosa_wichuraiana	Rw2G017180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	321, 1429
AccB1I	GGYRCC	1 cut(s)	382
AccI	GTMKAC	1 cut(s)	321
AccII	CGCG	2 cut(s)	186, 945
AciI	CCGC	3 cut(s)	184, 945, 1740
AclWI	GGATC	1 cut(s)	430
AcsI	RAATTY	4 cut(s)	1249, 1272, 1581, 1659
AcuI	CTGAAG	1 cut(s)	1127
AcyI	GRCGYC	2 cut(s)	318, 1426
AfaI	GTAC	3 cut(s)	574, 1054, 1482
AfiI	CCNNNNNNNGG	6 cut(s)	159, 656, 919, 1111, 1448, 1520
AflII	CTTAAG	1 cut(s)	599
AhdI	GACNNNNNGTC	1 cut(s)	870
AjnI	CCWGG	2 cut(s)	131, 1725
Alw21I	GWGCWC	4 cut(s)	196, 787, 1160, 1219
Alw26I	GTCTC	2 cut(s)	348, 1293
Alw44I	GTGCAC	1 cut(s)	783
AlwI	GGATC	1 cut(s)	430
Ama87I	CYCGRG	1 cut(s)	189
AoxI	GGCC	3 cut(s)	161, 210, 902
ApaLI	GTGCAC	1 cut(s)	783
ApoI	RAATTY	4 cut(s)	1249, 1272, 1581, 1659
AspLEI	GCGC	2 cut(s)	72, 188
AsuC2I	CCSGG	2 cut(s)	311, 746
AsuHPI	GGTGA	2 cut(s)	158, 821
AvaI	CYCGRG	1 cut(s)	189
BaeGI	GKGCMC	1 cut(s)	787
BanI	GGYRCC	1 cut(s)	382
BanII	GRGCYC	2 cut(s)	196, 1702
BbsI	GAAGAC	2 cut(s)	537, 1429
Bbv12I	GWGCWC	4 cut(s)	196, 787, 1160, 1219
BbvCI	CCTCAGC	1 cut(s)	914
BceAI	ACGGC	2 cut(s)	257, 370
BcgI	CGANNNNNNTGC	2 cut(s)	61, 95
BciT130I	CCWGG	2 cut(s)	133, 1727
BcnI	CCSGG	2 cut(s)	311, 746
BcoDI	GTCTC	2 cut(s)	348, 1293
BfaI	CTAG	4 cut(s)	159, 332, 867, 1023
BfmI	CTRYAG	2 cut(s)	535, 1056
BfrI	CTTAAG	1 cut(s)	599
BlpI	GCTNAGC	1 cut(s)	1159
Bme1390I	CCNGG	4 cut(s)	133, 311, 746, 1727
BmeRI	GACNNNNNGTC	1 cut(s)	870
BmeT110I	CYCGRG	1 cut(s)	189
BmiI	GGNNCC	3 cut(s)	384, 1243, 1701
BmrFI	CCNGG	4 cut(s)	133, 311, 746, 1727
BmsI	GCATC	5 cut(s)	670, 692, 906, 1357, 1551
BoxI	GACNNNNGTC	1 cut(s)	21
BpiI	GAAGAC	2 cut(s)	537, 1429
BpmI	CTGGAG	1 cut(s)	981
Bpu10I	CCTNAGC	1 cut(s)	914
Bpu1102I	GCTNAGC	1 cut(s)	1159
BpuEI	CTTGAG	4 cut(s)	629, 905, 1650, 1777
BpuMI	CCSGG	2 cut(s)	311, 746
BsaHI	GRCGYC	2 cut(s)	318, 1426
BsaJI	CCNNGG	3 cut(s)	132, 390, 1515
Bsc4I	CCNNNNNNNGG	6 cut(s)	159, 656, 919, 1111, 1448, 1520
Bse1I	ACTGG	1 cut(s)	1285
Bse3DI	GCAATG	1 cut(s)	848
BseBI	CCWGG	2 cut(s)	133, 1727
BseDI	CCNNGG	3 cut(s)	132, 390, 1515
BseGI	GGATG	7 cut(s)	355, 672, 1032, 1348, 1433, 1525, 1552
BseLI	CCNNNNNNNGG	6 cut(s)	159, 656, 919, 1111, 1448, 1520
BseMI	GCAATG	1 cut(s)	848
BseMII	CTCAG	9 cut(s)	359, 869, 928, 1097, 1173, 1277, 1304, 1637, 1685
BseNI	ACTGG	1 cut(s)	1285
BseRI	GAGGAG	4 cut(s)	241, 244, 596, 1431
BseSI	GKGCMC	1 cut(s)	787
Bsh1236I	CGCG	2 cut(s)	186, 945
Bsh1285I	CGRYCG	1 cut(s)	25
BshFI	GGCC	3 cut(s)	163, 212, 904
BshNI	GGYRCC	1 cut(s)	382
BsiEI	CGRYCG	1 cut(s)	25
BsiHKAI	GWGCWC	4 cut(s)	196, 787, 1160, 1219
BsiHKCI	CYCGRG	1 cut(s)	189
BsiSI	CCGG	2 cut(s)	311, 746
BslFI	GGGAC	2 cut(s)	29, 858
BslI	CCNNNNNNNGG	6 cut(s)	159, 656, 919, 1111, 1448, 1520
BsmAI	GTCTC	2 cut(s)	348, 1293
BsmBI	CGTCTC	1 cut(s)	348
BsmFI	GGGAC	2 cut(s)	29, 858
BsmI	GAATGC	1 cut(s)	1422
BsnI	GGCC	3 cut(s)	163, 212, 904
BsoBI	CYCGRG	1 cut(s)	189
Bsp1286I	GDGCHC	5 cut(s)	196, 787, 1160, 1219, 1702
Bsp143I	GATC	1 cut(s)	422
Bsp1720I	GCTNAGC	1 cut(s)	1159
Bsp19I	CCATGG	1 cut(s)	1515
BspACI	CCGC	3 cut(s)	184, 945, 1740
BspANI	GGCC	3 cut(s)	163, 212, 904
BspCNI	CTCAG	9 cut(s)	358, 868, 927, 1096, 1172, 1276, 1303, 1636, 1686
BspFNI	CGCG	2 cut(s)	186, 945
BspLI	GGNNCC	3 cut(s)	384, 1243, 1701
BspPI	GGATC	1 cut(s)	430
BspQI	GCTCTTC	1 cut(s)	201
BspT107I	GGYRCC	1 cut(s)	382
BspTI	CTTAAG	1 cut(s)	599
BsrDI	GCAATG	1 cut(s)	848
BsrI	ACTGG	1 cut(s)	1285
BssECI	CCNNGG	3 cut(s)	132, 390, 1515
BssMI	GATC	1 cut(s)	422
BssNI	GRCGYC	2 cut(s)	318, 1426
BssT1I	CCWWGG	2 cut(s)	390, 1515
Bst2UI	CCWGG	2 cut(s)	133, 1727
Bst4CI	ACNGT	5 cut(s)	23, 770, 1002, 1195, 1538
Bst6I	CTCTTC	3 cut(s)	201, 230, 262
BstACI	GRCGYC	2 cut(s)	318, 1426
BstAFI	CTTAAG	1 cut(s)	599
BstC8I	GCNNGC	3 cut(s)	43, 214, 993
BstDSI	CCRYGG	1 cut(s)	1515
BstF5I	GGATG	7 cut(s)	355, 672, 1032, 1348, 1433, 1525, 1552
BstFNI	CGCG	2 cut(s)	186, 945
BstHHI	GCGC	2 cut(s)	72, 188
BstKTI	GATC	1 cut(s)	425
BstMAI	GTCTC	2 cut(s)	348, 1293
BstMBI	GATC	1 cut(s)	422
BstMCI	CGRYCG	1 cut(s)	25
BstMWI	GCNNNNNNNGC	9 cut(s)	76, 104, 416, 889, 1264, 1303, 1393, 1402, 1570
BstNI	CCWGG	2 cut(s)	133, 1727
BstPAI	GACNNNNGTC	1 cut(s)	21
BstSCI	CCNGG	4 cut(s)	131, 309, 744, 1725
BstSFI	CTRYAG	2 cut(s)	535, 1056
BstSLI	GKGCMC	1 cut(s)	787
BstUI	CGCG	2 cut(s)	186, 945
BstV2I	GAAGAC	2 cut(s)	537, 1429
BsuRI	GGCC	3 cut(s)	163, 212, 904
BtgI	CCRYGG	1 cut(s)	1515
BtgZI	GCGATG	1 cut(s)	926
BtsCI	GGATG	7 cut(s)	355, 672, 1032, 1348, 1433, 1525, 1552
BtsI	GCAGTG	1 cut(s)	1160
BtsIMutI	CAGTG	1 cut(s)	1160
Cac8I	GCNNGC	3 cut(s)	43, 214, 993
CfoI	GCGC	2 cut(s)	72, 188
CseI	GACGC	1 cut(s)	383
Csp6I	GTAC	3 cut(s)	573, 1053, 1481
CviAII	CATG	7 cut(s)	75, 145, 492, 919, 1516, 1684, 1760
CviQI	GTAC	3 cut(s)	573, 1053, 1481
DpnI	GATC	1 cut(s)	424
DpnII	GATC	1 cut(s)	422
DriI	GACNNNNNGTC	1 cut(s)	870
Eam1104I	CTCTTC	3 cut(s)	201, 230, 262
Eam1105I	GACNNNNNGTC	1 cut(s)	870
EarI	CTCTTC	3 cut(s)	201, 230, 262
EciI	GGCGGA	1 cut(s)	1755
Ecl136II	GAGCTC	1 cut(s)	194
Eco130I	CCWWGG	2 cut(s)	390, 1515
Eco147I	AGGCCT	3 cut(s)	163, 212, 904
Eco24I	GRGCYC	2 cut(s)	196, 1702
Eco53kI	GAGCTC	1 cut(s)	194
Eco57I	CTGAAG	1 cut(s)	1127
Eco88I	CYCGRG	1 cut(s)	189
EcoICRI	GAGCTC	1 cut(s)	194
EcoRII	CCWGG	2 cut(s)	131, 1725
EcoT14I	CCWWGG	2 cut(s)	390, 1515
EcoT22I	ATGCAT	2 cut(s)	685, 1350
EcoT38I	GRGCYC	2 cut(s)	196, 1702
ErhI	CCWWGG	2 cut(s)	390, 1515
Esp3I	CGTCTC	1 cut(s)	348
FaeI	CATG	7 cut(s)	78, 148, 495, 922, 1519, 1687, 1763
FaqI	GGGAC	2 cut(s)	29, 858
FatI	CATG	7 cut(s)	74, 144, 491, 918, 1515, 1683, 1759
FauNDI	CATATG	1 cut(s)	690
FblI	GTMKAC	1 cut(s)	321
FokI	GGATG	7 cut(s)	362, 659, 1039, 1335, 1420, 1532, 1539
FriOI	GRGCYC	2 cut(s)	196, 1702
FspBI	CTAG	4 cut(s)	159, 332, 867, 1023
GlaI	GCGC	2 cut(s)	71, 187
GsuI	CTGGAG	1 cut(s)	981
HaeIII	GGCC	3 cut(s)	163, 212, 904
HapII	CCGG	2 cut(s)	311, 746
HgaI	GACGC	1 cut(s)	383
HhaI	GCGC	2 cut(s)	72, 188
Hin1I	GRCGYC	2 cut(s)	318, 1426
Hin1II	CATG	7 cut(s)	78, 148, 495, 922, 1519, 1687, 1763
Hin6I	GCGC	2 cut(s)	70, 186
HinP1I	GCGC	2 cut(s)	70, 186
HincII	GTYRAC	1 cut(s)	1430
HindII	GTYRAC	1 cut(s)	1430
HindIII	AAGCTT	2 cut(s)	138, 602
HpaII	CCGG	2 cut(s)	311, 746
HphI	GGTGA	2 cut(s)	158, 821
Hpy166II	GTNNAC	5 cut(s)	322, 759, 785, 1430, 1618
Hpy188I	TCNGA	6 cut(s)	442, 565, 858, 1507, 1695, 1768
Hpy8I	GTNNAC	5 cut(s)	322, 759, 785, 1430, 1618
Hpy99I	CGWCG	2 cut(s)	23, 320
HpyAV	CCTTC	8 cut(s)	121, 135, 1043, 1196, 1459, 1540, 1705, 1712
HpyCH4III	ACNGT	5 cut(s)	23, 770, 1002, 1195, 1538
HpyCH4IV	ACGT	5 cut(s)	63, 318, 571, 1383, 1426
HpyCH4V	TGCA	8 cut(s)	60, 301, 683, 785, 1297, 1348, 1360, 1564
HpyF10VI	GCNNNNNNNGC	9 cut(s)	76, 104, 416, 889, 1264, 1303, 1393, 1402, 1570
HpySE526I	ACGT	5 cut(s)	63, 318, 571, 1383, 1426
Hsp92I	GRCGYC	2 cut(s)	318, 1426
Hsp92II	CATG	7 cut(s)	78, 148, 495, 922, 1519, 1687, 1763
HspAI	GCGC	2 cut(s)	70, 186
Kzo9I	GATC	1 cut(s)	422
LguI	GCTCTTC	1 cut(s)	201
LmnI	GCTCC	5 cut(s)	309, 553, 1225, 1247, 1705
LweI	GCATC	5 cut(s)	670, 692, 906, 1357, 1551
MaeI	CTAG	4 cut(s)	159, 332, 867, 1023
MaeII	ACGT	5 cut(s)	63, 318, 571, 1383, 1426
MaeIII	GTNAC	6 cut(s)	29, 371, 809, 1086, 1142, 1509
MalI	GATC	1 cut(s)	424
MboI	GATC	1 cut(s)	422
MboII	GAAGA	8 cut(s)	94, 188, 217, 249, 252, 255, 537, 1434
MfeI	CAATTG	1 cut(s)	1068
MhlI	GDGCHC	5 cut(s)	196, 787, 1160, 1219, 1702
MlyI	GAGTC	4 cut(s)	143, 622, 880, 1757
MmeI	TCCRAC	1 cut(s)	202
Mph1103I	ATGCAT	2 cut(s)	685, 1350
MseI	TTAA	1 cut(s)	600
MslI	CAYNNNNRTG	1 cut(s)	1451
MspCI	CTTAAG	1 cut(s)	599
MspI	CCGG	2 cut(s)	311, 746
MspR9I	CCNGG	4 cut(s)	133, 311, 746, 1727
MunI	CAATTG	1 cut(s)	1068
Mva1269I	GAATGC	1 cut(s)	1422
MvaI	CCWGG	2 cut(s)	133, 1727
MvnI	CGCG	2 cut(s)	186, 945
MwoI	GCNNNNNNNGC	9 cut(s)	76, 104, 416, 889, 1264, 1303, 1393, 1402, 1570
NciI	CCSGG	2 cut(s)	311, 746
NcoI	CCATGG	1 cut(s)	1515
NdeI	CATATG	1 cut(s)	690
NdeII	GATC	1 cut(s)	422
NlaIII	CATG	7 cut(s)	78, 148, 495, 922, 1519, 1687, 1763
NlaIV	GGNNCC	3 cut(s)	384, 1243, 1701
NmeAIII	GCCGAG	1 cut(s)	60
NmuCI	GTSAC	2 cut(s)	371, 809
NsiI	ATGCAT	2 cut(s)	685, 1350
PaeR7I	CTCGAG	1 cut(s)	189
PceI	AGGCCT	3 cut(s)	163, 212, 904
PciSI	GCTCTTC	1 cut(s)	201
PctI	GAATGC	1 cut(s)	1422
PflFI	GACNNNGTC	1 cut(s)	317
PfoI	TCCNGGA	2 cut(s)	744, 1725
PleI	GAGTC	4 cut(s)	143, 621, 879, 1757
PpsI	GAGTC	4 cut(s)	143, 621, 879, 1757
PshAI	GACNNNNGTC	1 cut(s)	21
Psp124BI	GAGCTC	1 cut(s)	196
Psp6I	CCWGG	2 cut(s)	131, 1725
PspGI	CCWGG	2 cut(s)	131, 1725
PspN4I	GGNNCC	3 cut(s)	384, 1243, 1701
PspXI	VCTCGAGB	1 cut(s)	189
PsyI	GACNNNGTC	1 cut(s)	317
RsaI	GTAC	3 cut(s)	574, 1054, 1482
RsaNI	GTAC	3 cut(s)	573, 1053, 1481
RseI	CAYNNNNRTG	1 cut(s)	1451
SacI	GAGCTC	1 cut(s)	196
SapI	GCTCTTC	1 cut(s)	201
SaqAI	TTAA	1 cut(s)	600
Sau3AI	GATC	1 cut(s)	422
SchI	GAGTC	4 cut(s)	143, 622, 880, 1757
ScrFI	CCNGG	4 cut(s)	133, 311, 746, 1727
SduI	GDGCHC	5 cut(s)	196, 787, 1160, 1219, 1702
SfaNI	GCATC	5 cut(s)	670, 692, 906, 1357, 1551
SfcI	CTRYAG	2 cut(s)	535, 1056
Sfr274I	CTCGAG	1 cut(s)	189
SlaI	CTCGAG	1 cut(s)	189
SmiMI	CAYNNNNRTG	1 cut(s)	1451
SmlI	CTYRAG	6 cut(s)	189, 599, 608, 884, 1665, 1792
SmoI	CTYRAG	6 cut(s)	189, 599, 608, 884, 1665, 1792
SseBI	AGGCCT	3 cut(s)	163, 212, 904
SsiI	CCGC	3 cut(s)	184, 945, 1740
SspI	AATATT	1 cut(s)	1039
SspMI	CTAG	4 cut(s)	159, 332, 867, 1023
SstI	GAGCTC	1 cut(s)	196
StuI	AGGCCT	3 cut(s)	163, 212, 904
StyD4I	CCNGG	4 cut(s)	131, 309, 744, 1725
StyI	CCWWGG	2 cut(s)	390, 1515
TaaI	ACNGT	5 cut(s)	23, 770, 1002, 1195, 1538
TaiI	ACGT	5 cut(s)	66, 321, 574, 1386, 1429
TaqI	TCGA	5 cut(s)	51, 190, 204, 1321, 1595
Tru1I	TTAA	1 cut(s)	600
Tru9I	TTAA	1 cut(s)	600
TscAI	CASTG	1 cut(s)	1160
TseFI	GTSAC	2 cut(s)	371, 809
Tsp45I	GTSAC	2 cut(s)	371, 809
TspDTI	ATGAA	4 cut(s)	133, 707, 1740, 1785
TspGWI	ACGGA	1 cut(s)	1293
TspRI	CASTG	1 cut(s)	1160
Tth111I	GACNNNGTC	1 cut(s)	317
Vha464I	CTTAAG	1 cut(s)	599
VneI	GTGCAC	1 cut(s)	783
XapI	RAATTY	4 cut(s)	1249, 1272, 1581, 1659
XbaI	TCTAGA	1 cut(s)	331
XhoI	CTCGAG	1 cut(s)	189
XmiI	GTMKAC	1 cut(s)	321
XspI	CTAG	4 cut(s)	159, 332, 867, 1023
ZraI	GACGTC	2 cut(s)	319, 1427
Zsp2I	ATGCAT	2 cut(s)	685, 1350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.