Rmu_sc0032518.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032518.1
OG0005483
Physical Location & Seq
Forward (+)
274 .. 767
494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032518.1_g000001.1.cds

Sequence Viewer

Length: 414 bp
atggctttactcggagacgacggccgtggctacgagctggcctgtaagctcgagtcctgcaacgtctggcgcacctggctcggcgactccaactacgccaccttcgtccacgtcctctcctccccctccacctgggactccttcatgcgatccgacgactccaaatctagggctcagatccaactccaactccgcgcccgtgccctcctcttcgacaaggcctgcgtctctctcttcctccgccccgattccgattccaataattcctcctcttcttcttcgctcgccgtttccaagctcaatcccacctatttgcagctgcatgctgatgacgtgtacttcactctcgagaactctcccgcagaaggagttcaggctcaacagcgcgaggcctccaaggtatgctctttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromo_TP

Protein Analysis

137

Amino Acids

15.22

Weight (kDa)

5.26

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 195, 387
AciI CCGC 3 cut(s) 193, 241, 360
AclWI GGATC 2 cut(s) 144, 172
AcoI YGGCCR 1 cut(s) 22
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 132, 168, 365
AflIII ACRYGT 1 cut(s) 333
AjiI CACGTC 2 cut(s) 112, 334
AjnI CCWGG 2 cut(s) 74, 131
AluBI AGCT 4 cut(s) 37, 49, 298, 319
AluI AGCT 4 cut(s) 37, 49, 298, 319
Alw26I GTCTC 2 cut(s) 9, 232
AlwI GGATC 2 cut(s) 144, 172
Ama87I CYCGRG 2 cut(s) 50, 347
AoxI GGCC 4 cut(s) 22, 39, 219, 390
ApeKI GCWGC 2 cut(s) 316, 319
Asp700I GAANNNNTTC 1 cut(s) 369
AspLEI GCGC 3 cut(s) 72, 197, 387
AvaI CYCGRG 2 cut(s) 50, 347
BaeGI GKGCMC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 175
BbvI GCAGC 2 cut(s) 306, 328
BceAI ACGGC 3 cut(s) 9, 37, 272
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 2 cut(s) 76, 133
BcoDI GTCTC 2 cut(s) 9, 232
BfaI CTAG 2 cut(s) 168, 412
BisI GCNGC 2 cut(s) 317, 320
BlsI GCNGC 2 cut(s) 318, 321
Bme1390I CCNGG 2 cut(s) 76, 133
BmeT110I CYCGRG 2 cut(s) 50, 347
BmgBI CACGTC 2 cut(s) 112, 334
BmrFI CCNGG 2 cut(s) 76, 133
BsaJI CCNNGG 3 cut(s) 25, 132, 396
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 174, 204
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 168, 365
BseBI CCWGG 2 cut(s) 76, 133
BseDI CCNNGG 3 cut(s) 25, 132, 396
BseLI CCNNNNNNNGG 3 cut(s) 132, 168, 365
BseMII CTCAG 1 cut(s) 188
BseRI GAGGAG 3 cut(s) 109, 197, 259
BseSI GKGCMC 1 cut(s) 205
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 306, 328
Bsh1236I CGCG 2 cut(s) 195, 387
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 4 cut(s) 24, 41, 221, 392
BsiEI CGRYCG 1 cut(s) 25
BsiHKCI CYCGRG 2 cut(s) 50, 347
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 3 cut(s) 132, 168, 365
BsmAI GTCTC 2 cut(s) 9, 232
BsmBI CGTCTC 2 cut(s) 9, 232
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 4 cut(s) 24, 41, 221, 392
BsoBI CYCGRG 2 cut(s) 50, 347
Bsp1286I GDGCHC 2 cut(s) 175, 205
Bsp143I GATC 2 cut(s) 149, 177
BspACI CCGC 3 cut(s) 193, 241, 360
BspANI GGCC 4 cut(s) 24, 41, 221, 392
BspCNI CTCAG 1 cut(s) 187
BspFNI CGCG 2 cut(s) 195, 387
BspPI GGATC 2 cut(s) 144, 172
BssECI CCNNGG 3 cut(s) 25, 132, 396
BssMI GATC 2 cut(s) 149, 177
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 76, 133
Bst6I CTCTTC 3 cut(s) 215, 239, 277
BstC8I GCNNGC 4 cut(s) 39, 223, 285, 324
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 25
BstFNI CGCG 2 cut(s) 195, 387
BstHHI GCGC 3 cut(s) 72, 197, 387
BstKTI GATC 2 cut(s) 152, 180
BstMAI GTCTC 2 cut(s) 9, 232
BstMBI GATC 2 cut(s) 149, 177
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstNI CCWGG 2 cut(s) 76, 133
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 74, 131
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 2 cut(s) 195, 387
BstV1I GCAGC 2 cut(s) 306, 328
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 4 cut(s) 24, 41, 221, 392
BtgI CCRYGG 1 cut(s) 25
BtrI CACGTC 2 cut(s) 112, 334
Cac8I GCNNGC 4 cut(s) 39, 223, 285, 324
CfoI GCGC 3 cut(s) 72, 197, 387
CseI GACGC 1 cut(s) 214
Csp6I GTAC 1 cut(s) 337
CviAII CATG 2 cut(s) 145, 323
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 1 cut(s) 174
DpnI GATC 2 cut(s) 151, 179
DpnII GATC 2 cut(s) 149, 177
EaeI YGGCCR 1 cut(s) 22
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 3 cut(s) 215, 239, 277
EarI CTCTTC 3 cut(s) 215, 239, 277
EciI GGCGGA 1 cut(s) 230
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 396
Eco147I AGGCCT 2 cut(s) 221, 392
Eco24I GRGCYC 1 cut(s) 175
Eco52I CGGCCG 1 cut(s) 22
Eco88I CYCGRG 2 cut(s) 50, 347
EcoRII CCWGG 2 cut(s) 74, 131
EcoT14I CCWWGG 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 2 cut(s) 9, 232
FaeI CATG 2 cut(s) 148, 326
FaiI YATR 3 cut(s) 146, 324, 403
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 144, 322
FauI CCCGC 1 cut(s) 367
Fnu4HI GCNGC 2 cut(s) 317, 320
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 2 cut(s) 317, 320
FspBI CTAG 2 cut(s) 168, 412
GlaI GCGC 3 cut(s) 71, 196, 386
GluI GCNGC 2 cut(s) 317, 320
HaeIII GGCC 4 cut(s) 24, 41, 221, 392
HgaI GACGC 1 cut(s) 214
HhaI GCGC 3 cut(s) 72, 197, 387
Hin1II CATG 2 cut(s) 148, 326
Hin6I GCGC 3 cut(s) 70, 195, 385
HinP1I GCGC 3 cut(s) 70, 195, 385
HinfI GANTC 6 cut(s) 53, 86, 137, 158, 248, 254
Hpy166II GTNNAC 2 cut(s) 109, 337
Hpy188I TCNGA 4 cut(s) 14, 154, 177, 253
Hpy188III TCNNGA 2 cut(s) 347, 349
Hpy8I GTNNAC 2 cut(s) 109, 337
Hpy99I CGWCG 2 cut(s) 23, 158
HpyAV CCTTC 3 cut(s) 112, 151, 359
HpyCH4IV ACGT 3 cut(s) 63, 111, 333
HpyCH4V TGCA 3 cut(s) 60, 316, 322
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 3 cut(s) 63, 111, 333
Hsp92II CATG 2 cut(s) 148, 326
HspAI GCGC 3 cut(s) 70, 195, 385
Kzo9I GATC 2 cut(s) 149, 177
Lsp1109I GCAGC 2 cut(s) 306, 328
MaeI CTAG 2 cut(s) 168, 412
MaeII ACGT 3 cut(s) 63, 111, 333
MalI GATC 2 cut(s) 151, 179
MboI GATC 2 cut(s) 149, 177
MboII GAAGA 5 cut(s) 202, 226, 264, 267, 270
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 2 cut(s) 175, 205
MluCI AATT 1 cut(s) 262
MlyI GAGTC 4 cut(s) 62, 80, 131, 152
MmeI TCCRAC 4 cut(s) 114, 177, 205, 211
MroXI GAANNNNTTC 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 319
MspR9I CCNGG 2 cut(s) 76, 133
MvaI CCWGG 2 cut(s) 76, 133
MvnI CGCG 2 cut(s) 195, 387
MwoI GCNNNNNNNGC 1 cut(s) 76
NdeII GATC 2 cut(s) 149, 177
NlaIII CATG 2 cut(s) 148, 326
NmeAIII GCCGAG 1 cut(s) 60
NspI RCATGY 1 cut(s) 326
PaeI GCATGC 1 cut(s) 326
PaeR7I CTCGAG 2 cut(s) 50, 347
PceI AGGCCT 2 cut(s) 221, 392
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 2 cut(s) 248, 254
PkrI GCNGC 2 cut(s) 318, 321
PleI GAGTC 4 cut(s) 61, 80, 131, 152
PpsI GAGTC 4 cut(s) 61, 80, 131, 152
Psp6I CCWGG 2 cut(s) 74, 131
PspGI CCWGG 2 cut(s) 74, 131
PspXI VCTCGAGB 1 cut(s) 50
PsuI RGATCY 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 319
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 327
SatI GCNGC 2 cut(s) 317, 320
Sau3AI GATC 2 cut(s) 149, 177
SchI GAGTC 4 cut(s) 62, 80, 131, 152
ScrFI CCNGG 2 cut(s) 76, 133
SduI GDGCHC 2 cut(s) 175, 205
Sfr274I CTCGAG 2 cut(s) 50, 347
SlaI CTCGAG 2 cut(s) 50, 347
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 2 cut(s) 50, 347
SmoI CTYRAG 2 cut(s) 50, 347
SphI GCATGC 1 cut(s) 326
Sse9I AATT 1 cut(s) 262
SseBI AGGCCT 2 cut(s) 221, 392
SsiI CCGC 3 cut(s) 193, 241, 360
SspMI CTAG 2 cut(s) 168, 412
StuI AGGCCT 2 cut(s) 221, 392
StyD4I CCNGG 2 cut(s) 74, 131
StyI CCWWGG 1 cut(s) 396
TaiI ACGT 3 cut(s) 66, 114, 336
TaqI TCGA 3 cut(s) 51, 213, 348
TasI AATT 1 cut(s) 262
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 2 cut(s) 248, 254
TseI GCWGC 2 cut(s) 316, 319
TspDTI ATGAA 1 cut(s) 133
XceI RCATGY 1 cut(s) 326
XhoI CTCGAG 2 cut(s) 50, 347
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 2 cut(s) 168, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.