Rmu_sc0032519.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032519.1
OG0005483
Physical Location & Seq
Forward (+)
220 .. 746
527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032519.1_g000001.1.cds

Sequence Viewer

Length: 447 bp
atgactagcattctcagtccttcctctccaaaaatggctttactcggagacgacggccgtggctacgagctggcctgtaagctcgagtcctgcaacgtctggcgcacctggctcggcgactccaactactccaccttcgtccacgtcctctcctccccctccacctgggactccttcatgcgatccgacgactccaaatctagggctcagatccaactccaactccgcgcccgtgccctcctcttcgacaaggcctgcgtctctctcttcctccgccccgattccgattccaataattcctcctctccttcttcgctcgccgtttccaagctcaatcccacctatttgcagctgcatgctgatgacgtgtacttcactctcgagaactctcccacagaaggagttcaggctcaacagcgcgaggcctccaaggtatgctctttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromo_TP

Protein Analysis

148

Amino Acids

16.41

Weight (kDa)

5.6

Isoelectric Point (pI)

64.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 228, 420
AciI CCGC 2 cut(s) 226, 274
AclWI GGATC 2 cut(s) 177, 205
AcoI YGGCCR 1 cut(s) 55
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 3 cut(s) 165, 201, 398
AflIII ACRYGT 1 cut(s) 366
AjiI CACGTC 2 cut(s) 145, 367
AjnI CCWGG 2 cut(s) 107, 164
AluBI AGCT 4 cut(s) 70, 82, 331, 352
AluI AGCT 4 cut(s) 70, 82, 331, 352
Alw26I GTCTC 2 cut(s) 42, 265
AlwI GGATC 2 cut(s) 177, 205
Ama87I CYCGRG 2 cut(s) 83, 380
AoxI GGCC 4 cut(s) 55, 72, 252, 423
ApeKI GCWGC 2 cut(s) 349, 352
Asp700I GAANNNNTTC 1 cut(s) 402
AspLEI GCGC 3 cut(s) 105, 230, 420
AvaI CYCGRG 2 cut(s) 83, 380
BaeGI GKGCMC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 208
BbvI GCAGC 2 cut(s) 339, 361
BceAI ACGGC 3 cut(s) 42, 70, 305
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 2 cut(s) 109, 166
BcoDI GTCTC 2 cut(s) 42, 265
BfaI CTAG 3 cut(s) 6, 201, 445
BisI GCNGC 2 cut(s) 350, 353
BlsI GCNGC 2 cut(s) 351, 354
Bme1390I CCNGG 2 cut(s) 109, 166
BmeT110I CYCGRG 2 cut(s) 83, 380
BmgBI CACGTC 2 cut(s) 145, 367
BmrFI CCNGG 2 cut(s) 109, 166
BsaJI CCNNGG 3 cut(s) 58, 165, 429
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 207, 237
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 201, 398
BseBI CCWGG 2 cut(s) 109, 166
BseDI CCNNGG 3 cut(s) 58, 165, 429
BseLI CCNNNNNNNGG 3 cut(s) 165, 201, 398
BseMII CTCAG 2 cut(s) 28, 221
BseRI GAGGAG 3 cut(s) 142, 230, 292
BseSI GKGCMC 1 cut(s) 238
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 339, 361
Bsh1236I CGCG 2 cut(s) 228, 420
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 4 cut(s) 57, 74, 254, 425
BsiEI CGRYCG 1 cut(s) 58
BsiHKCI CYCGRG 2 cut(s) 83, 380
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 3 cut(s) 165, 201, 398
BsmAI GTCTC 2 cut(s) 42, 265
BsmBI CGTCTC 2 cut(s) 42, 265
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 9
BsnI GGCC 4 cut(s) 57, 74, 254, 425
BsoBI CYCGRG 2 cut(s) 83, 380
Bsp1286I GDGCHC 2 cut(s) 208, 238
Bsp143I GATC 2 cut(s) 182, 210
BspACI CCGC 2 cut(s) 226, 274
BspANI GGCC 4 cut(s) 57, 74, 254, 425
BspCNI CTCAG 2 cut(s) 27, 220
BspFNI CGCG 2 cut(s) 228, 420
BspPI GGATC 2 cut(s) 177, 205
BssECI CCNNGG 3 cut(s) 58, 165, 429
BssMI GATC 2 cut(s) 182, 210
BssT1I CCWWGG 1 cut(s) 429
Bst2UI CCWGG 2 cut(s) 109, 166
Bst6I CTCTTC 2 cut(s) 248, 272
BstC8I GCNNGC 4 cut(s) 72, 256, 318, 357
BstDEI CTNAG 2 cut(s) 14, 207
BstDSI CCRYGG 1 cut(s) 58
BstFNI CGCG 2 cut(s) 228, 420
BstHHI GCGC 3 cut(s) 105, 230, 420
BstKTI GATC 2 cut(s) 185, 213
BstMAI GTCTC 2 cut(s) 42, 265
BstMBI GATC 2 cut(s) 182, 210
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstNI CCWGG 2 cut(s) 109, 166
BstNSI RCATGY 1 cut(s) 359
BstSCI CCNGG 2 cut(s) 107, 164
BstSLI GKGCMC 1 cut(s) 238
BstUI CGCG 2 cut(s) 228, 420
BstV1I GCAGC 2 cut(s) 339, 361
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 4 cut(s) 57, 74, 254, 425
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 2 cut(s) 145, 367
Cac8I GCNNGC 4 cut(s) 72, 256, 318, 357
CfoI GCGC 3 cut(s) 105, 230, 420
CseI GACGC 1 cut(s) 247
Csp6I GTAC 1 cut(s) 370
CviAII CATG 2 cut(s) 178, 356
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 2 cut(s) 14, 207
DpnI GATC 2 cut(s) 184, 212
DpnII GATC 2 cut(s) 182, 210
EaeI YGGCCR 1 cut(s) 55
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 248, 272
EarI CTCTTC 2 cut(s) 248, 272
EciI GGCGGA 1 cut(s) 263
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 429
Eco147I AGGCCT 2 cut(s) 254, 425
Eco24I GRGCYC 1 cut(s) 208
Eco52I CGGCCG 1 cut(s) 55
Eco88I CYCGRG 2 cut(s) 83, 380
EcoRII CCWGG 2 cut(s) 107, 164
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 429
Esp3I CGTCTC 2 cut(s) 42, 265
FaeI CATG 2 cut(s) 181, 359
FaiI YATR 3 cut(s) 179, 357, 436
FaqI GGGAC 1 cut(s) 182
FatI CATG 2 cut(s) 177, 355
Fnu4HI GCNGC 2 cut(s) 350, 353
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 2 cut(s) 350, 353
FspBI CTAG 3 cut(s) 6, 201, 445
GlaI GCGC 3 cut(s) 104, 229, 419
GluI GCNGC 2 cut(s) 350, 353
HaeIII GGCC 4 cut(s) 57, 74, 254, 425
HgaI GACGC 1 cut(s) 247
HhaI GCGC 3 cut(s) 105, 230, 420
Hin1II CATG 2 cut(s) 181, 359
Hin6I GCGC 3 cut(s) 103, 228, 418
HinP1I GCGC 3 cut(s) 103, 228, 418
HinfI GANTC 6 cut(s) 86, 119, 170, 191, 281, 287
Hpy166II GTNNAC 2 cut(s) 142, 370
Hpy188I TCNGA 4 cut(s) 47, 187, 210, 286
Hpy188III TCNNGA 2 cut(s) 380, 382
Hpy8I GTNNAC 2 cut(s) 142, 370
Hpy99I CGWCG 2 cut(s) 56, 191
HpyAV CCTTC 5 cut(s) 30, 145, 184, 318, 392
HpyCH4IV ACGT 3 cut(s) 96, 144, 366
HpyCH4V TGCA 3 cut(s) 93, 349, 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpyF3I CTNAG 2 cut(s) 14, 207
HpySE526I ACGT 3 cut(s) 96, 144, 366
Hsp92II CATG 2 cut(s) 181, 359
HspAI GCGC 3 cut(s) 103, 228, 418
Kzo9I GATC 2 cut(s) 182, 210
Lsp1109I GCAGC 2 cut(s) 339, 361
MaeI CTAG 3 cut(s) 6, 201, 445
MaeII ACGT 3 cut(s) 96, 144, 366
MalI GATC 2 cut(s) 184, 212
MboI GATC 2 cut(s) 182, 210
MboII GAAGA 3 cut(s) 235, 259, 303
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 2 cut(s) 208, 238
MluCI AATT 1 cut(s) 295
MlyI GAGTC 4 cut(s) 95, 113, 164, 185
MmeI TCCRAC 4 cut(s) 147, 210, 238, 244
MroXI GAANNNNTTC 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 352
MspR9I CCNGG 2 cut(s) 109, 166
Mva1269I GAATGC 1 cut(s) 9
MvaI CCWGG 2 cut(s) 109, 166
MvnI CGCG 2 cut(s) 228, 420
MwoI GCNNNNNNNGC 1 cut(s) 109
NdeII GATC 2 cut(s) 182, 210
NlaIII CATG 2 cut(s) 181, 359
NmeAIII GCCGAG 1 cut(s) 93
NspI RCATGY 1 cut(s) 359
PaeI GCATGC 1 cut(s) 359
PaeR7I CTCGAG 2 cut(s) 83, 380
PceI AGGCCT 2 cut(s) 254, 425
PctI GAATGC 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 281, 287
PkrI GCNGC 2 cut(s) 351, 354
PleI GAGTC 4 cut(s) 94, 113, 164, 185
PpsI GAGTC 4 cut(s) 94, 113, 164, 185
Psp6I CCWGG 2 cut(s) 107, 164
PspGI CCWGG 2 cut(s) 107, 164
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 352
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 360
SatI GCNGC 2 cut(s) 350, 353
Sau3AI GATC 2 cut(s) 182, 210
SchI GAGTC 4 cut(s) 95, 113, 164, 185
ScrFI CCNGG 2 cut(s) 109, 166
SduI GDGCHC 2 cut(s) 208, 238
Sfr274I CTCGAG 2 cut(s) 83, 380
SlaI CTCGAG 2 cut(s) 83, 380
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 2 cut(s) 83, 380
SmoI CTYRAG 2 cut(s) 83, 380
SphI GCATGC 1 cut(s) 359
Sse9I AATT 1 cut(s) 295
SseBI AGGCCT 2 cut(s) 254, 425
SsiI CCGC 2 cut(s) 226, 274
SspMI CTAG 3 cut(s) 6, 201, 445
StuI AGGCCT 2 cut(s) 254, 425
StyD4I CCNGG 2 cut(s) 107, 164
StyI CCWWGG 1 cut(s) 429
TaiI ACGT 3 cut(s) 99, 147, 369
TaqI TCGA 3 cut(s) 84, 246, 381
TasI AATT 1 cut(s) 295
TatI WGTACW 1 cut(s) 369
TfiI GAWTC 2 cut(s) 281, 287
TseI GCWGC 2 cut(s) 349, 352
TspDTI ATGAA 1 cut(s) 166
XceI RCATGY 1 cut(s) 359
XhoI CTCGAG 2 cut(s) 83, 380
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 3 cut(s) 6, 201, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.