Rmu_sc0003994.1_g000015

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003994.1
OG0005483
Physical Location & Seq
Reverse (-)
86672 .. 86890
219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003994.1_g000015.1.cds

Sequence Viewer

Length: 219 bp
atgactagcattctcagtccttcctctccaaaaatggctttactcggagacgacggccgtggctatgagctggcctgtaagctcgagtcctgcaacgtctggcgcacctggctcggcgactccaactacgccaccttcgtccacgtcctctcctccccctccacctgggactccttcatgcgatccgacgactccaaatctagggctcagatgttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromo_TP

Protein Analysis

72

Amino Acids

8.07

Weight (kDa)

5.58

Isoelectric Point (pI)

74.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 177
AcoI YGGCCR 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 165, 201
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 2 cut(s) 107, 164
AluBI AGCT 2 cut(s) 70, 82
AluI AGCT 2 cut(s) 70, 82
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 1 cut(s) 177
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 2 cut(s) 55, 72
AspLEI GCGC 1 cut(s) 105
AvaI CYCGRG 1 cut(s) 83
BanII GRGCYC 1 cut(s) 208
BceAI ACGGC 2 cut(s) 42, 70
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 2 cut(s) 109, 166
BcoDI GTCTC 1 cut(s) 42
BfaI CTAG 2 cut(s) 6, 201
Bme1390I CCNGG 2 cut(s) 109, 166
BmeT110I CYCGRG 1 cut(s) 83
BmgBI CACGTC 1 cut(s) 145
BmrFI CCNGG 2 cut(s) 109, 166
BsaJI CCNNGG 2 cut(s) 58, 165
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 201
BseBI CCWGG 2 cut(s) 109, 166
BseDI CCNNGG 2 cut(s) 58, 165
BseLI CCNNNNNNNGG 2 cut(s) 165, 201
BseMII CTCAG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 142
BseX3I CGGCCG 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 57, 74
BsiEI CGRYCG 1 cut(s) 58
BsiHKCI CYCGRG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 165, 201
BsmAI GTCTC 1 cut(s) 42
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 9
BsnI GGCC 2 cut(s) 57, 74
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 1 cut(s) 182
BspANI GGCC 2 cut(s) 57, 74
BspCNI CTCAG 1 cut(s) 27
BspPI GGATC 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 58, 165
BssMI GATC 1 cut(s) 182
Bst2UI CCWGG 2 cut(s) 109, 166
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 2 cut(s) 14, 207
BstDSI CCRYGG 1 cut(s) 58
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 1 cut(s) 185
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 1 cut(s) 182
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstNI CCWGG 2 cut(s) 109, 166
BstSCI CCNGG 2 cut(s) 107, 164
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 2 cut(s) 57, 74
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 72
CfoI GCGC 1 cut(s) 105
CviAII CATG 1 cut(s) 178
CviJI RGCY 8 cut(s) 38, 57, 63, 70, 74, 82, 112, 206
CviKI_1 RGCY 8 cut(s) 38, 57, 63, 70, 74, 82, 112, 206
DdeI CTNAG 2 cut(s) 14, 207
DpnI GATC 1 cut(s) 184
DpnII GATC 1 cut(s) 182
EaeI YGGCCR 1 cut(s) 55
EagI CGGCCG 1 cut(s) 55
EclXI CGGCCG 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 208
Eco52I CGGCCG 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 107, 164
EcoT38I GRGCYC 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 1 cut(s) 181
FaiI YATR 2 cut(s) 66, 179
FaqI GGGAC 1 cut(s) 182
FatI CATG 1 cut(s) 177
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 2 cut(s) 6, 201
GlaI GCGC 1 cut(s) 104
HaeIII GGCC 2 cut(s) 57, 74
HhaI GCGC 1 cut(s) 105
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 4 cut(s) 86, 119, 170, 191
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 3 cut(s) 47, 187, 210
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 2 cut(s) 56, 191
HpyAV CCTTC 3 cut(s) 30, 145, 184
HpyCH4IV ACGT 2 cut(s) 96, 144
HpyCH4V TGCA 1 cut(s) 93
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpyF3I CTNAG 2 cut(s) 14, 207
HpySE526I ACGT 2 cut(s) 96, 144
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 1 cut(s) 103
Kzo9I GATC 1 cut(s) 182
LpnPI CCDG 8 cut(s) 56, 85, 88, 94, 103, 121, 151, 178
MaeI CTAG 2 cut(s) 6, 201
MaeII ACGT 2 cut(s) 96, 144
MalI GATC 1 cut(s) 184
MboI GATC 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 208
MlyI GAGTC 4 cut(s) 95, 113, 164, 185
MmeI TCCRAC 2 cut(s) 147, 210
MnlI CCTC 4 cut(s) 34, 158, 163, 169
MspR9I CCNGG 2 cut(s) 109, 166
Mva1269I GAATGC 1 cut(s) 9
MvaI CCWGG 2 cut(s) 109, 166
MwoI GCNNNNNNNGC 1 cut(s) 109
NdeII GATC 1 cut(s) 182
NlaIII CATG 1 cut(s) 181
NmeAIII GCCGAG 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 9
PleI GAGTC 4 cut(s) 94, 113, 164, 185
PpsI GAGTC 4 cut(s) 94, 113, 164, 185
Psp6I CCWGG 2 cut(s) 107, 164
PspGI CCWGG 2 cut(s) 107, 164
PspXI VCTCGAGB 1 cut(s) 83
Sau3AI GATC 1 cut(s) 182
SchI GAGTC 4 cut(s) 95, 113, 164, 185
ScrFI CCNGG 2 cut(s) 109, 166
SduI GDGCHC 1 cut(s) 208
SetI ASST 7 cut(s) 72, 84, 99, 110, 137, 147, 167
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
SspMI CTAG 2 cut(s) 6, 201
StyD4I CCNGG 2 cut(s) 107, 164
TaiI ACGT 2 cut(s) 99, 147
TaqI TCGA 1 cut(s) 84
TspDTI ATGAA 1 cut(s) 166
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 2 cut(s) 6, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.