RLG00000017855

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005483
Physical Location & Seq
Reverse (-)
22347909 .. 22351490
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017855

Sequence Viewer

Length: 1377 bp
ATGGCTTTACTCGGAGACGACGGCCGTGGCTACGAGCTGGCCTGTAAGCTCGAGTCCTGCAACGTCTGGCGCACCTGGCTCGGCGACTCCAACTACGCCACCTTCGTCCACTTCCTCTCCTCCCCCTCCACCTGGGACTCCTTCATGCGATCCGACGACTCCAAATCTAGGGCTCAGATCCAACTCCAACTCCGCGCCCGTGCCCTCCTCTTCGACAAGGCCTGCGTCTCTCTCTTCCTCCGCCCCGATTCCGATTCCAATTCCTCCTCTTCTTCTTCTTCTTCGCTCGCCGTTTCCAAGCTCAGTCCCACCTATTTGCAGTTGCATGCTGACGACGTGTACTTCAGTCTCGAGAATTCTTCCACAGAAGGAGTTCAGGCTCAACAGCGCGAGGCCTCCAAGATTCAGTCAAAAACAGCTTTTGGTTTTGGATCAAGATATGGAGAATCTGAAATTGATAATAAGCCACCAAGGTTTAAAAATGAAGAGCTGCCTGAGACCTGGTATAATCAGGTTATTGAGAGACATAAGGCTAGCAAACCGCATAGGTTGTCATCAGCAGACCGAGAATCAGAGAAACGTACTCCAGAGGAGATGTCTACTTATCTTAAGCTTGCTGTGAAGCATAAGAAAAGGTGTTTAGCATTTAAAGAAGATCAGTATGTGGGATATGGAAATCCCCTCCTAGAAAATGCATCACAGAATCCACATTCTGTTTTAGATGGTAGTAGTTCAATTGATCATGAAGCATCTTTTTTCCCAGAAACAATGTTTACGTTGAACTGTGTTCCTGATAGTGCACTTCCACCTATGAATAGAGTGCAAGACGACCAAAAAGTTGAATTTTATGGAGTTCTTGATACGTTACCTCAGGCTTTGACTAGGAGTCCCGTTATGCTTGAGAGGCTTGGTATAAGACCTGAATACCTTAACATGGATCAAGGAGGAAGCTTACATCGTGGAAAGAATGGGTCTGCAGGGAACAAAAGTTGTCTTACTCATGAGCAAGCGGCACAGTTATCTCAAAAGGTAATAGCCCGGATGCTGACAAATGTGGGATTTGAAGGTTCCTCTGAAGTTCCAATTGAAGTTTTCTCTCAGTTGCTAAGCTGTCATATCCGTAAATTGGGTAGCAGCTTGAAAGTTCTTTCTGATAGTTACAGAAAGCAGTGTTCAGCAATTGAACTATTGAAGATGTTCCTTCAAACAGTGGGATATAGTAATTTTGGCCCTCTAGCTGATCAAGTCAAGGATGGCTCCAGGAATTTCCATCAACAAAGTCAGCAGCAAATTCATGGAATGCAGTCACAGTTGCTGCCACAGCACCAGAACCCTATTCGACTACCCCAACAAGTATTTCTCCCTTCAATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromo_TP

Protein Analysis

459

Amino Acids

51.37

Weight (kDa)

6.9

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromo_TP PF07524 336 - 406 9.3e-06 Bromodomain associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 599
AccII CGCG 2 cut(s) 195, 390
AciI CCGC 4 cut(s) 193, 241, 542, 1010
AclWI GGATC 4 cut(s) 144, 172, 439, 945
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 4 cut(s) 355, 842, 1264, 1290
AcuI CTGAAG 2 cut(s) 328, 1095
AfaI GTAC 2 cut(s) 341, 583
AfiI CCNNNNNNNGG 4 cut(s) 132, 168, 934, 1126
AflII CTTAAG 1 cut(s) 608
AflIII ACRYGT 1 cut(s) 336
AhdI GACNNNNNGTC 1 cut(s) 885
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 4 cut(s) 74, 131, 500, 1259
AjuI GAANNNNNNNTTGG 2 cut(s) 1341, 1373
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 5 cut(s) 9, 232, 353, 491, 517
Alw44I GTGCAC 1 cut(s) 798
AlwI GGATC 4 cut(s) 144, 172, 439, 945
AlwNI CAGNNNCTG 1 cut(s) 1315
Ama87I CYCGRG 2 cut(s) 50, 350
AoxI GGCC 5 cut(s) 22, 39, 219, 393, 1228
ApaLI GTGCAC 1 cut(s) 798
ApeKI GCWGC 4 cut(s) 490, 1134, 1285, 1315
ApoI RAATTY 4 cut(s) 355, 842, 1264, 1290
AseI ATTAAT 1 cut(s) 1371
Asp700I GAANNNNTTC 2 cut(s) 372, 1196
AspLEI GCGC 3 cut(s) 72, 197, 390
AspS9I GGNCC 1 cut(s) 1229
AsuC2I CCSGG 1 cut(s) 1039
AsuNHI GCTAGC 1 cut(s) 533
AvaI CYCGRG 2 cut(s) 50, 350
AxyI CCTNAGG 1 cut(s) 870
BaeGI GKGCMC 2 cut(s) 205, 802
BanII GRGCYC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 4 cut(s) 477, 1146, 1297, 1302
BccI CCATC 3 cut(s) 716, 1247, 1278
BceAI ACGGC 3 cut(s) 9, 37, 275
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 4 cut(s) 76, 133, 502, 1261
BclI TGATCA 2 cut(s) 739, 1240
BcnI CCSGG 1 cut(s) 1039
BcoDI GTCTC 5 cut(s) 9, 232, 353, 491, 517
BfaI CTAG 5 cut(s) 168, 534, 686, 882, 1235
BfmI CTRYAG 1 cut(s) 975
BfrI CTTAAG 1 cut(s) 608
BisI GCNGC 5 cut(s) 491, 1011, 1135, 1286, 1316
BlpI GCTNAGC 1 cut(s) 1106
BlsI GCNGC 5 cut(s) 492, 1012, 1136, 1287, 1317
Bme1390I CCNGG 5 cut(s) 76, 133, 502, 1039, 1261
BmeRI GACNNNNNGTC 1 cut(s) 885
BmeT110I CYCGRG 2 cut(s) 50, 350
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 1229
BmiI GGNNCC 2 cut(s) 1069, 1258
BmrFI CCNGG 5 cut(s) 76, 133, 502, 1039, 1261
BmsI GCATC 3 cut(s) 704, 758, 1032
BmtI GCTAGC 1 cut(s) 537
BpmI CTGGAG 2 cut(s) 570, 1243
Bpu1102I GCTNAGC 1 cut(s) 1106
BpuEI CTTGAG 1 cut(s) 920
BpuMI CCSGG 1 cut(s) 1039
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 3 cut(s) 25, 132, 470
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 174, 204
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 168, 934, 1126
Bse21I CCTNAGG 1 cut(s) 870
BseBI CCWGG 4 cut(s) 76, 133, 502, 1261
BseDI CCNNGG 3 cut(s) 25, 132, 470
BseGI GGATG 2 cut(s) 1047, 1258
BseLI CCNNNNNNNGG 4 cut(s) 132, 168, 934, 1126
BseMII CTCAG 5 cut(s) 188, 316, 486, 884, 1112
BseRI GAGGAG 4 cut(s) 109, 197, 256, 605
BseSI GKGCMC 2 cut(s) 205, 802
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 4 cut(s) 477, 1146, 1297, 1302
Bsh1236I CGCG 2 cut(s) 195, 390
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 5 cut(s) 24, 41, 221, 395, 1230
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 802
BsiHKCI CYCGRG 2 cut(s) 50, 350
BsiSI CCGG 1 cut(s) 1039
BslFI GGGAC 3 cut(s) 149, 291, 873
BslI CCNNNNNNNGG 4 cut(s) 132, 168, 934, 1126
BsmAI GTCTC 5 cut(s) 9, 232, 353, 491, 517
BsmBI CGTCTC 2 cut(s) 9, 232
BsmFI GGGAC 3 cut(s) 149, 291, 873
BsmI GAATGC 1 cut(s) 1305
BsnI GGCC 5 cut(s) 24, 41, 221, 395, 1230
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 2 cut(s) 50, 350
Bsp1286I GDGCHC 3 cut(s) 175, 205, 802
Bsp143I GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
Bsp1720I GCTNAGC 1 cut(s) 1106
BspACI CCGC 4 cut(s) 193, 241, 542, 1010
BspANI GGCC 5 cut(s) 24, 41, 221, 395, 1230
BspCNI CTCAG 5 cut(s) 187, 315, 487, 883, 1111
BspFNI CGCG 2 cut(s) 195, 390
BspHI TCATGA 2 cut(s) 742, 1000
BspLI GGNNCC 2 cut(s) 1069, 1258
BspMAI CTGCAG 1 cut(s) 979
BspOI GCTAGC 1 cut(s) 537
BspPI GGATC 4 cut(s) 144, 172, 439, 945
BspQI GCTCTTC 1 cut(s) 480
BspTI CTTAAG 1 cut(s) 608
BspTNI GGTCTC 1 cut(s) 491
BssECI CCNNGG 3 cut(s) 25, 132, 470
BssMI GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
BssT1I CCWWGG 1 cut(s) 470
Bst2UI CCWGG 4 cut(s) 76, 133, 502, 1261
Bst4CI ACNGT 4 cut(s) 785, 1017, 1210, 1311
Bst6I CTCTTC 4 cut(s) 215, 239, 274, 480
BstAFI CTTAAG 1 cut(s) 608
BstC8I GCNNGC 7 cut(s) 39, 223, 288, 327, 535, 615, 1008
BstDEI CTNAG 6 cut(s) 174, 302, 495, 870, 1098, 1106
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 2 cut(s) 1047, 1258
BstFNI CGCG 2 cut(s) 195, 390
BstHHI GCGC 3 cut(s) 72, 197, 390
BstKTI GATC 7 cut(s) 152, 180, 434, 658, 742, 940, 1243
BstMAI GTCTC 5 cut(s) 9, 232, 353, 491, 517
BstMBI GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 76, 904, 1321
BstNI CCWGG 4 cut(s) 76, 133, 502, 1261
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 5 cut(s) 74, 131, 500, 1037, 1259
BstSFI CTRYAG 1 cut(s) 975
BstSLI GKGCMC 2 cut(s) 205, 802
BstUI CGCG 2 cut(s) 195, 390
BstV1I GCAGC 4 cut(s) 477, 1146, 1297, 1302
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BstZI CGGCCG 1 cut(s) 22
Bsu36I CCTNAGG 1 cut(s) 870
BsuRI GGCC 5 cut(s) 24, 41, 221, 395, 1230
BtgI CCRYGG 1 cut(s) 25
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 2 cut(s) 1047, 1258
BtsI GCAGTG 1 cut(s) 1175
BtsIMutI CAGTG 2 cut(s) 1175, 1215
Cac8I GCNNGC 7 cut(s) 39, 223, 288, 327, 535, 615, 1008
CaiI CAGNNNCTG 1 cut(s) 1315
CciI TCATGA 2 cut(s) 742, 1000
CfoI GCGC 3 cut(s) 72, 197, 390
Cfr13I GGNCC 1 cut(s) 1229
CseI GACGC 1 cut(s) 214
CsiI ACCWGGT 1 cut(s) 500
Csp6I GTAC 2 cut(s) 340, 582
CviAII CATG 6 cut(s) 145, 326, 743, 934, 1001, 1295
CviQI GTAC 2 cut(s) 340, 582
DdeI CTNAG 6 cut(s) 174, 302, 495, 870, 1098, 1106
DpnI GATC 7 cut(s) 151, 179, 433, 657, 741, 939, 1242
DpnII GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
DraI TTTAAA 2 cut(s) 478, 649
DriI GACNNNNNGTC 1 cut(s) 885
EaeI YGGCCR 1 cut(s) 22
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 4 cut(s) 215, 239, 274, 480
Eam1105I GACNNNNNGTC 1 cut(s) 885
EarI CTCTTC 4 cut(s) 215, 239, 274, 480
EciI GGCGGA 1 cut(s) 230
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 470
Eco147I AGGCCT 2 cut(s) 221, 395
Eco24I GRGCYC 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 491
Eco52I CGGCCG 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 328, 1095
Eco81I CCTNAGG 1 cut(s) 870
Eco88I CYCGRG 2 cut(s) 50, 350
EcoRI GAATTC 1 cut(s) 355
EcoRII CCWGG 4 cut(s) 74, 131, 500, 1259
EcoT14I CCWWGG 1 cut(s) 470
EcoT22I ATGCAT 1 cut(s) 697
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 470
Esp3I CGTCTC 2 cut(s) 9, 232
FaeI CATG 6 cut(s) 148, 329, 746, 937, 1004, 1298
FaqI GGGAC 3 cut(s) 149, 291, 873
FatI CATG 6 cut(s) 144, 325, 742, 933, 1000, 1294
FbaI TGATCA 2 cut(s) 739, 1240
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 5 cut(s) 491, 1011, 1135, 1286, 1316
FokI GGATG 2 cut(s) 1054, 1265
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 5 cut(s) 491, 1011, 1135, 1286, 1316
FspBI CTAG 5 cut(s) 168, 534, 686, 882, 1235
GlaI GCGC 3 cut(s) 71, 196, 389
GluI GCNGC 5 cut(s) 491, 1011, 1135, 1286, 1316
GsuI CTGGAG 2 cut(s) 570, 1243
HaeIII GGCC 5 cut(s) 24, 41, 221, 395, 1230
HapII CCGG 1 cut(s) 1039
HgaI GACGC 1 cut(s) 214
HhaI GCGC 3 cut(s) 72, 197, 390
Hin1II CATG 6 cut(s) 148, 329, 746, 937, 1004, 1298
Hin6I GCGC 3 cut(s) 70, 195, 388
HinP1I GCGC 3 cut(s) 70, 195, 388
HindIII AAGCTT 2 cut(s) 611, 949
HpaII CCGG 1 cut(s) 1039
Hpy166II GTNNAC 5 cut(s) 109, 340, 600, 774, 800
Hpy188I TCNGA 8 cut(s) 14, 154, 177, 253, 451, 574, 1075, 1153
Hpy188III TCNNGA 8 cut(s) 350, 352, 435, 587, 743, 791, 857, 1001
Hpy8I GTNNAC 5 cut(s) 109, 340, 600, 774, 800
Hpy99I CGWCG 3 cut(s) 23, 158, 338
HpyAV CCTTC 6 cut(s) 112, 151, 362, 1058, 1211, 1374
HpyCH4III ACNGT 4 cut(s) 785, 1017, 1210, 1311
HpyCH4IV ACGT 5 cut(s) 63, 336, 580, 776, 863
HpyCH4V TGCA 8 cut(s) 60, 319, 325, 695, 800, 823, 977, 1303
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 904, 1321
HpyF3I CTNAG 6 cut(s) 174, 302, 495, 870, 1098, 1106
HpySE526I ACGT 5 cut(s) 63, 336, 580, 776, 863
Hsp92II CATG 6 cut(s) 148, 329, 746, 937, 1004, 1298
HspAI GCGC 3 cut(s) 70, 195, 388
Ksp22I TGATCA 2 cut(s) 739, 1240
Kzo9I GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
LguI GCTCTTC 1 cut(s) 480
LmnI GCTCC 1 cut(s) 1262
Lsp1109I GCAGC 4 cut(s) 477, 1146, 1297, 1302
LweI GCATC 3 cut(s) 704, 758, 1032
MabI ACCWGGT 1 cut(s) 500
MaeI CTAG 5 cut(s) 168, 534, 686, 882, 1235
MaeII ACGT 5 cut(s) 63, 336, 580, 776, 863
MaeIII GTNAC 3 cut(s) 864, 1157, 1305
MalI GATC 7 cut(s) 151, 179, 433, 657, 741, 939, 1242
MboI GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
MfeI CAATTG 3 cut(s) 735, 1083, 1179
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 3 cut(s) 175, 205, 802
MlyI GAGTC 5 cut(s) 62, 80, 131, 152, 895
MmeI TCCRAC 4 cut(s) 114, 177, 205, 211
Mph1103I ATGCAT 1 cut(s) 697
MroXI GAANNNNTTC 2 cut(s) 372, 1196
MseI TTAA 5 cut(s) 477, 609, 648, 930, 1371
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 1 cut(s) 1039
MspR9I CCNGG 5 cut(s) 76, 133, 502, 1039, 1261
MunI CAATTG 3 cut(s) 735, 1083, 1179
Mva1269I GAATGC 1 cut(s) 1305
MvaI CCWGG 4 cut(s) 76, 133, 502, 1261
MvnI CGCG 2 cut(s) 195, 390
MwoI GCNNNNNNNGC 3 cut(s) 76, 904, 1321
NciI CCSGG 1 cut(s) 1039
NdeII GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 6 cut(s) 148, 329, 746, 937, 1004, 1298
NlaIV GGNNCC 2 cut(s) 1069, 1258
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 1 cut(s) 1305
NsiI ATGCAT 1 cut(s) 697
NspI RCATGY 1 cut(s) 329
PaeI GCATGC 1 cut(s) 329
PaeR7I CTCGAG 2 cut(s) 50, 350
PagI TCATGA 2 cut(s) 742, 1000
PceI AGGCCT 2 cut(s) 221, 395
PciSI GCTCTTC 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 1305
PdmI GAANNNNTTC 2 cut(s) 372, 1196
PfeI GAWTC 6 cut(s) 248, 254, 403, 446, 569, 703
PfoI TCCNGGA 1 cut(s) 1259
PkrI GCNGC 5 cut(s) 492, 1012, 1136, 1287, 1317
PleI GAGTC 5 cut(s) 61, 80, 131, 152, 894
PpsI GAGTC 5 cut(s) 61, 80, 131, 152, 894
PshBI ATTAAT 1 cut(s) 1371
Psp6I CCWGG 4 cut(s) 74, 131, 500, 1259
PspGI CCWGG 4 cut(s) 74, 131, 500, 1259
PspN4I GGNNCC 2 cut(s) 1069, 1258
PspPI GGNCC 1 cut(s) 1229
PspXI VCTCGAGB 1 cut(s) 50
PstI CTGCAG 1 cut(s) 979
PstNI CAGNNNCTG 1 cut(s) 1315
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 2 cut(s) 341, 583
RsaNI GTAC 2 cut(s) 340, 582
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 5 cut(s) 477, 609, 648, 930, 1371
SatI GCNGC 5 cut(s) 491, 1011, 1135, 1286, 1316
Sau3AI GATC 7 cut(s) 149, 177, 431, 655, 739, 937, 1240
Sau96I GGNCC 1 cut(s) 1229
SchI GAGTC 5 cut(s) 62, 80, 131, 152, 895
ScrFI CCNGG 5 cut(s) 76, 133, 502, 1039, 1261
SduI GDGCHC 3 cut(s) 175, 205, 802
SexAI ACCWGGT 1 cut(s) 500
SfaNI GCATC 3 cut(s) 704, 758, 1032
SfcI CTRYAG 1 cut(s) 975
Sfr274I CTCGAG 2 cut(s) 50, 350
SlaI CTCGAG 2 cut(s) 50, 350
SmlI CTYRAG 4 cut(s) 50, 350, 608, 899
SmoI CTYRAG 4 cut(s) 50, 350, 608, 899
SphI GCATGC 1 cut(s) 329
SseBI AGGCCT 2 cut(s) 221, 395
SsiI CCGC 4 cut(s) 193, 241, 542, 1010
SspMI CTAG 5 cut(s) 168, 534, 686, 882, 1235
StuI AGGCCT 2 cut(s) 221, 395
StyD4I CCNGG 5 cut(s) 74, 131, 500, 1037, 1259
StyI CCWWGG 1 cut(s) 470
TaaI ACNGT 4 cut(s) 785, 1017, 1210, 1311
TaiI ACGT 5 cut(s) 66, 339, 583, 779, 866
TaqI TCGA 4 cut(s) 51, 213, 351, 1339
TaqII GACCGA 1 cut(s) 579
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 1013
TfiI GAWTC 6 cut(s) 248, 254, 403, 446, 569, 703
Tru1I TTAA 5 cut(s) 477, 609, 648, 930, 1371
Tru9I TTAA 5 cut(s) 477, 609, 648, 930, 1371
TscAI CASTG 2 cut(s) 1175, 1215
TseFI GTSAC 1 cut(s) 1305
TseI GCWGC 4 cut(s) 490, 1134, 1285, 1315
Tsp45I GTSAC 1 cut(s) 1305
TspDTI ATGAA 5 cut(s) 133, 498, 759, 827, 1283
TspGWI ACGGA 1 cut(s) 1109
TspRI CASTG 2 cut(s) 1175, 1215
Vha464I CTTAAG 1 cut(s) 608
VneI GTGCAC 1 cut(s) 798
VspI ATTAAT 1 cut(s) 1371
XapI RAATTY 4 cut(s) 355, 842, 1264, 1290
XceI RCATGY 1 cut(s) 329
XhoI CTCGAG 2 cut(s) 50, 350
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 2 cut(s) 372, 1196
XspI CTAG 5 cut(s) 168, 534, 686, 882, 1235
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.