Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0005483

Physical Location & Seq

Genomic Coordinates Reverse (-)

22834446 .. 22867051

Gene Structure 32606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG225400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGCAGATTCAGTCAAAAACAGCTTTTGGTTTTGGATCAAGATATGGAGAATCTGAAATTGATAATAAGCCACCAAGGTTTAAAAATGAAGAGCTGCCTGAGACCTGGTATAATCAGGTTATTGAGAGACATAGGGCTAGCAAACCGCATAGGTTGTCATCAGCAGACCGAGAATCAGAGAAACGTACTCCAGAGGAGATGTCTGCTTATCTTAAGCTTGCTGTGAAGCATAAGAAAAGGTGTTTAGCATTCAAAGAAGATCAGTATGTGGCATATGGAAATCCCCTCCAAGAAAATGCATCACAGAATCCACATTCTGTTTTAGATGGTAGTAGTTCAATTGATCATGAAGCATCTTTTTTCCCAGAAACAATGTTTACGTTGAACTGTGTTCCTGATAGTGCACTTCCACCTATGAATAGAGTGCAAGACGACCAAAAAGTTGAATTTTATGGAGTTCTTGATACGTTACCTCAGGCTTTGACTAGGAGTCCCGTTATGCTTGAGAGGCTTGGTATAAGACCTGAATACCTTAACATGGATCAAGGAGGAAGCTTACATCGTGGAAAGAACGGGTCTGCAGGGAACAAAAGTTGTCTTACTCATGAGCAAGCGGCACAGTTATCTCAAAAGGTAATAGCCCGGATGCTGACAAATGTGGGATTTGAAGGTTCCTCAGAAGTTCCAATTGAAGTTTTCTCTCAGTTGCTAAGCTGTCATATCCGTAAATTGGGTAGCAGCTTGAAAGTTCTTACTGATAGTTACAGAAAGCAGTGTTCAGCAATTGAACTATTAAAGATGTTCCTTCAAACAGTGGGATATAGTAATTTTGGCCCTCTAGCTGATCAAGTCAAGGATGGCTCCAGGAATTTCCATCAACAAAGTCAGCAGCAAATTCATGGAATGCAGTCACAGTTGCAGCCACAGCACCAGAACCCAATTCGACTACCCCAACAAATATCGAGACAAATGCATCCGCAGATGCAGCAGATTCAGCAAATGCAGCAAATTGCCCAATCCAAGAATGTGCCATTTCAGCAACAGCAGCAGATGGAAAGAATGCGAAGACGTCAACCATCTACTCGTGCTGGTATGGACATGGTACAGGACAGACCAATGGTCCAGGTCAAGATTGAAGCTCCATCAGAGTTACCCATGGATAGTAATGCCTTCAACAGTTTCAACAACAGACATCCACAATTGCAGTTCAGGCAGCAGCAAATTCCTGCAATGTCAAATCCTACAATGCAAAATGTCCCTGCTCAGTCAGGGAATCAGTTTAGACAGATATCTCCTTCACAAATTGCACAAATTCAATCACAGAACGCTGGTGTTCTTAGGGCTCGACCGGTGAAGGTTGAAGGTTTCCAGGAACTAATGGGTGGAGATGCTTCATCTAAGCATGATTCAGATGAAAATCGGCTGACCTCCCCAAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bromo_TP

Protein Analysis

479

Amino Acids

54.37

Weight (kDa)

9.15

Isoelectric Point (pI)

64.74

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005483)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65540
fragaria_vesca	FvH4_1g19490 FvH4_1g19490
malus_domestica	MD01G1040900.v1.1 MD15G1310400.v1.1
prunus_persica	Prupe.6G203300_v2.0.a1
pyrus_communis	pycom01g06850 pycom15g27420
rosa_chinensis	RchiOBHm_Chr2g0110871 RchiOBHm_Chr2g0110941
rosa_laevigata	RLG00000017847 RLG00000017849 RLG00000017853 RLG00000017855
rosa_multiflora	Rmu_sc0003994.1_g000015 Rmu_sc0003994.1_g000016 Rmu_sc0032518.1_g000001 Rmu_sc0032519.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132780 Rroxscaffold_2G00132790
rosa_rugosa	Rorug02G0170300 Rorug02G0170300
rosa_samantha	Rh2AG222300 Rh2BG233100 Rh2BG233800 Rh2CG225100 Rh2CG225400 Rh2DG230300
rosa_wichuraiana	Rw2G017180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1072
AciI	CCGC	3 cut(s)	146, 614, 977
AclWI	GGATC	2 cut(s)	43, 549
AcsI	RAATTY	5 cut(s)	446, 868, 894, 1221, 1311
AcyI	GRCGYC	1 cut(s)	1069
AfaI	GTAC	2 cut(s)	187, 1104
AfiI	CCNNNNNNNGG	2 cut(s)	538, 730
AflII	CTTAAG	1 cut(s)	212
AgeI	ACCGGT	1 cut(s)	1348
AhdI	GACNNNNNGTC	2 cut(s)	489, 1118
AjnI	CCWGG	4 cut(s)	104, 863, 1122, 1368
AluBI	AGCT	8 cut(s)	23, 94, 217, 555, 714, 741, 842, 1139
AluI	AGCT	8 cut(s)	23, 94, 217, 555, 714, 741, 842, 1139
Alw21I	GWGCWC	1 cut(s)	406
Alw26I	GTCTC	3 cut(s)	95, 121, 958
Alw44I	GTGCAC	1 cut(s)	402
AlwI	GGATC	2 cut(s)	43, 549
AoxI	GGCC	1 cut(s)	832
ApaLI	GTGCAC	1 cut(s)	402
ApeKI	GCWGC	9 cut(s)	94, 738, 889, 919, 985, 1003, 1045, 1213, 1216
ApoI	RAATTY	5 cut(s)	446, 868, 894, 1221, 1311
AsiGI	ACCGGT	1 cut(s)	1348
AspS9I	GGNCC	2 cut(s)	833, 1120
AsuC2I	CCSGG	1 cut(s)	643
AsuHPI	GGTGA	1 cut(s)	1363
AsuNHI	GCTAGC	1 cut(s)	137
AvaII	GGWCC	1 cut(s)	1120
AxyI	CCTNAGG	1 cut(s)	474
BaeGI	GKGCMC	1 cut(s)	406
BanII	GRGCYC	1 cut(s)	1345
BauI	CACGAG	1 cut(s)	1083
BbsI	GAAGAC	1 cut(s)	1072
Bbv12I	GWGCWC	1 cut(s)	406
BbvI	GCAGC	9 cut(s)	81, 750, 901, 931, 997, 1015, 1057, 1225, 1228
BccI	CCATC	6 cut(s)	320, 851, 882, 1045, 1084, 1150
BcgI	CGANNNNNNTGC	2 cut(s)	952, 986
BciT130I	CCWGG	4 cut(s)	106, 865, 1124, 1370
BclI	TGATCA	2 cut(s)	343, 844
BcnI	CCSGG	1 cut(s)	643
BcoDI	GTCTC	3 cut(s)	95, 121, 958
BfaI	CTAG	3 cut(s)	138, 486, 839
BfmI	CTRYAG	1 cut(s)	579
BfrI	CTTAAG	1 cut(s)	212
BlpI	GCTNAGC	1 cut(s)	710
Bme1390I	CCNGG	5 cut(s)	106, 643, 865, 1124, 1370
Bme18I	GGWCC	1 cut(s)	1120
BmeRI	GACNNNNNGTC	2 cut(s)	489, 1118
BmgT120I	GGNCC	2 cut(s)	833, 1120
BmiI	GGNNCC	2 cut(s)	673, 862
BmrFI	CCNGG	5 cut(s)	106, 643, 865, 1124, 1370
BmsI	GCATC	6 cut(s)	308, 362, 636, 972, 982, 1378
BmtI	GCTAGC	1 cut(s)	141
BpiI	GAAGAC	1 cut(s)	1072
BpmI	CTGGAG	2 cut(s)	174, 847
Bpu1102I	GCTNAGC	1 cut(s)	710
BpuEI	CTTGAG	1 cut(s)	524
BpuMI	CCSGG	1 cut(s)	643
BsaBI	GATNNNNATC	1 cut(s)	1416
BsaHI	GRCGYC	1 cut(s)	1069
BsaI	GGTCTC	1 cut(s)	95
BsaJI	CCNNGG	2 cut(s)	74, 1155
BsaWI	WCCGGW	1 cut(s)	1348
Bsc4I	CCNNNNNNNGG	2 cut(s)	538, 730
Bse118I	RCCGGY	1 cut(s)	1348
Bse21I	CCTNAGG	1 cut(s)	474
Bse3DI	GCAATG	1 cut(s)	1236
Bse8I	GATNNNNATC	1 cut(s)	1416
BseBI	CCWGG	4 cut(s)	106, 865, 1124, 1370
BseDI	CCNNGG	2 cut(s)	74, 1155
BseGI	GGATG	4 cut(s)	651, 862, 973, 1192
BseJI	GATNNNNATC	1 cut(s)	1416
BseLI	CCNNNNNNNGG	2 cut(s)	538, 730
BseMI	GCAATG	1 cut(s)	1236
BseMII	CTCAG	5 cut(s)	90, 488, 690, 716, 1277
BseRI	GAGGAG	1 cut(s)	209
BseSI	GKGCMC	1 cut(s)	406
BseXI	GCAGC	9 cut(s)	81, 750, 901, 931, 997, 1015, 1057, 1225, 1228
Bsh1285I	CGRYCG	1 cut(s)	1349
BshFI	GGCC	1 cut(s)	834
BshTI	ACCGGT	1 cut(s)	1348
BsiEI	CGRYCG	1 cut(s)	1349
BsiHKAI	GWGCWC	1 cut(s)	406
BsiSI	CCGG	2 cut(s)	643, 1349
BslFI	GGGAC	2 cut(s)	477, 1241
BslI	CCNNNNNNNGG	2 cut(s)	538, 730
BsmAI	GTCTC	3 cut(s)	95, 121, 958
BsmFI	GGGAC	2 cut(s)	477, 1241
BsmI	GAATGC	3 cut(s)	248, 909, 1065
BsnI	GGCC	1 cut(s)	834
Bso31I	GGTCTC	1 cut(s)	95
Bsp1286I	GDGCHC	2 cut(s)	406, 1345
Bsp143I	GATC	5 cut(s)	35, 259, 343, 541, 844
Bsp1720I	GCTNAGC	1 cut(s)	710
Bsp19I	CCATGG	1 cut(s)	1155
BspACI	CCGC	3 cut(s)	146, 614, 977
BspANI	GGCC	1 cut(s)	834
BspCNI	CTCAG	5 cut(s)	91, 487, 689, 715, 1276
BspHI	TCATGA	2 cut(s)	346, 604
BspLI	GGNNCC	2 cut(s)	673, 862
BspMAI	CTGCAG	1 cut(s)	583
BspOI	GCTAGC	1 cut(s)	141
BspPI	GGATC	2 cut(s)	43, 549
BspQI	GCTCTTC	1 cut(s)	84
BspTI	CTTAAG	1 cut(s)	212
BspTNI	GGTCTC	1 cut(s)	95
BsrDI	GCAATG	1 cut(s)	1236
BsrFI	RCCGGY	1 cut(s)	1348
BssAI	RCCGGY	1 cut(s)	1348
BssECI	CCNNGG	2 cut(s)	74, 1155
BssMI	GATC	5 cut(s)	35, 259, 343, 541, 844
BssNI	GRCGYC	1 cut(s)	1069
BssSI	CACGAG	1 cut(s)	1083
BssT1I	CCWWGG	2 cut(s)	74, 1155
Bst2BI	CACGAG	1 cut(s)	1083
Bst2UI	CCWGG	4 cut(s)	106, 865, 1124, 1370
Bst4CI	ACNGT	5 cut(s)	389, 621, 814, 915, 1178
Bst6I	CTCTTC	1 cut(s)	84
BstACI	GRCGYC	1 cut(s)	1069
BstAFI	CTTAAG	1 cut(s)	212
BstC8I	GCNNGC	3 cut(s)	139, 219, 612
BstDEI	CTNAG	8 cut(s)	99, 474, 676, 702, 710, 1263, 1337, 1398
BstDSI	CCRYGG	1 cut(s)	1155
BstF5I	GGATG	4 cut(s)	651, 862, 973, 1192
BstKTI	GATC	5 cut(s)	38, 262, 346, 544, 847
BstMAI	GTCTC	3 cut(s)	95, 121, 958
BstMBI	GATC	5 cut(s)	35, 259, 343, 541, 844
BstMCI	CGRYCG	1 cut(s)	1349
BstMWI	GCNNNNNNNGC	8 cut(s)	508, 925, 985, 994, 1003, 1036, 1045, 1210
BstNI	CCWGG	4 cut(s)	106, 865, 1124, 1370
BstSCI	CCNGG	5 cut(s)	104, 641, 863, 1122, 1368
BstSFI	CTRYAG	1 cut(s)	579
BstSLI	GKGCMC	1 cut(s)	406
BstV1I	GCAGC	9 cut(s)	81, 750, 901, 931, 997, 1015, 1057, 1225, 1228
BstV2I	GAAGAC	1 cut(s)	1072
Bsu36I	CCTNAGG	1 cut(s)	474
BsuRI	GGCC	1 cut(s)	834
BtgI	CCRYGG	1 cut(s)	1155
BtsCI	GGATG	4 cut(s)	651, 862, 973, 1192
BtsI	GCAGTG	1 cut(s)	779
BtsIMutI	CAGTG	2 cut(s)	779, 819
Cac8I	GCNNGC	3 cut(s)	139, 219, 612
CciI	TCATGA	2 cut(s)	346, 604
Cfr10I	RCCGGY	1 cut(s)	1348
Cfr13I	GGNCC	2 cut(s)	833, 1120
CsiI	ACCWGGT	1 cut(s)	104
Csp6I	GTAC	2 cut(s)	186, 1103
CspAI	ACCGGT	1 cut(s)	1348
CviAII	CATG	7 cut(s)	347, 538, 605, 899, 1099, 1156, 1403
CviQI	GTAC	2 cut(s)	186, 1103
DdeI	CTNAG	8 cut(s)	99, 474, 676, 702, 710, 1263, 1337, 1398
DpnI	GATC	5 cut(s)	37, 261, 345, 543, 846
DpnII	GATC	5 cut(s)	35, 259, 343, 541, 844
DraI	TTTAAA	1 cut(s)	82
DriI	GACNNNNNGTC	2 cut(s)	489, 1118
Eam1104I	CTCTTC	1 cut(s)	84
Eam1105I	GACNNNNNGTC	2 cut(s)	489, 1118
EarI	CTCTTC	1 cut(s)	84
Eco130I	CCWWGG	2 cut(s)	74, 1155
Eco24I	GRGCYC	1 cut(s)	1345
Eco31I	GGTCTC	1 cut(s)	95
Eco32I	GATATC	1 cut(s)	1290
Eco47I	GGWCC	1 cut(s)	1120
Eco81I	CCTNAGG	1 cut(s)	474
EcoRII	CCWGG	4 cut(s)	104, 863, 1122, 1368
EcoRV	GATATC	1 cut(s)	1290
EcoT14I	CCWWGG	2 cut(s)	74, 1155
EcoT22I	ATGCAT	2 cut(s)	301, 975
EcoT38I	GRGCYC	1 cut(s)	1345
ErhI	CCWWGG	2 cut(s)	74, 1155
FaeI	CATG	7 cut(s)	350, 541, 608, 902, 1102, 1159, 1406
FaqI	GGGAC	2 cut(s)	477, 1241
FatI	CATG	7 cut(s)	346, 537, 604, 898, 1098, 1155, 1402
FauNDI	CATATG	1 cut(s)	274
FbaI	TGATCA	2 cut(s)	343, 844
FokI	GGATG	4 cut(s)	658, 869, 960, 1179
FriOI	GRGCYC	1 cut(s)	1345
FspBI	CTAG	3 cut(s)	138, 486, 839
GsuI	CTGGAG	2 cut(s)	174, 847
HaeIII	GGCC	1 cut(s)	834
HapII	CCGG	2 cut(s)	643, 1349
Hin1I	GRCGYC	1 cut(s)	1069
Hin1II	CATG	7 cut(s)	350, 541, 608, 902, 1102, 1159, 1406
HincII	GTYRAC	1 cut(s)	1073
HindII	GTYRAC	1 cut(s)	1073
HindIII	AAGCTT	2 cut(s)	215, 553
HinfI	GANTC	8 cut(s)	7, 50, 173, 307, 490, 991, 1273, 1406
HpaII	CCGG	2 cut(s)	643, 1349
HphI	GGTGA	1 cut(s)	1363
Hpy166II	GTNNAC	3 cut(s)	378, 404, 1073
Hpy188I	TCNGA	5 cut(s)	55, 178, 679, 1147, 1411
Hpy188III	TCNNGA	8 cut(s)	39, 191, 347, 395, 461, 605, 963, 1129
Hpy8I	GTNNAC	3 cut(s)	378, 404, 1073
HpyAV	CCTTC	6 cut(s)	662, 815, 1180, 1305, 1348, 1355
HpyCH4III	ACNGT	5 cut(s)	389, 621, 814, 915, 1178
HpyCH4IV	ACGT	4 cut(s)	184, 380, 467, 1069
HpyF10VI	GCNNNNNNNGC	8 cut(s)	508, 925, 985, 994, 1003, 1036, 1045, 1210
HpyF3I	CTNAG	8 cut(s)	99, 474, 676, 702, 710, 1263, 1337, 1398
HpySE526I	ACGT	4 cut(s)	184, 380, 467, 1069
Hsp92I	GRCGYC	1 cut(s)	1069
Hsp92II	CATG	7 cut(s)	350, 541, 608, 902, 1102, 1159, 1406
Ksp22I	TGATCA	2 cut(s)	343, 844
Kzo9I	GATC	5 cut(s)	35, 259, 343, 541, 844
LguI	GCTCTTC	1 cut(s)	84
LmnI	GCTCC	2 cut(s)	866, 1144
Lsp1109I	GCAGC	9 cut(s)	81, 750, 901, 931, 997, 1015, 1057, 1225, 1228
LweI	GCATC	6 cut(s)	308, 362, 636, 972, 982, 1378
MabI	ACCWGGT	1 cut(s)	104
MaeI	CTAG	3 cut(s)	138, 486, 839
MaeII	ACGT	4 cut(s)	184, 380, 467, 1069
MaeIII	GTNAC	4 cut(s)	468, 761, 909, 1149
MalI	GATC	5 cut(s)	37, 261, 345, 543, 846
MboI	GATC	5 cut(s)	35, 259, 343, 541, 844
MboII	GAAGA	3 cut(s)	101, 269, 1077
MfeI	CAATTG	4 cut(s)	339, 687, 783, 1199
MhlI	GDGCHC	2 cut(s)	406, 1345
MlyI	GAGTC	1 cut(s)	499
MnlI	CCTC	8 cut(s)	187, 296, 483, 501, 542, 685, 846, 1438
Mph1103I	ATGCAT	2 cut(s)	301, 975
MseI	TTAA	4 cut(s)	81, 213, 534, 794
MspCI	CTTAAG	1 cut(s)	212
MspI	CCGG	2 cut(s)	643, 1349
MspR9I	CCNGG	5 cut(s)	106, 643, 865, 1124, 1370
MunI	CAATTG	4 cut(s)	339, 687, 783, 1199
Mva1269I	GAATGC	3 cut(s)	248, 909, 1065
MvaI	CCWGG	4 cut(s)	106, 865, 1124, 1370
MwoI	GCNNNNNNNGC	8 cut(s)	508, 925, 985, 994, 1003, 1036, 1045, 1210
NciI	CCSGG	1 cut(s)	643
NcoI	CCATGG	1 cut(s)	1155
NdeI	CATATG	1 cut(s)	274
NdeII	GATC	5 cut(s)	35, 259, 343, 541, 844
NheI	GCTAGC	1 cut(s)	137
NlaIII	CATG	7 cut(s)	350, 541, 608, 902, 1102, 1159, 1406
NlaIV	GGNNCC	2 cut(s)	673, 862
NmuCI	GTSAC	1 cut(s)	909
NsiI	ATGCAT	2 cut(s)	301, 975
PagI	TCATGA	2 cut(s)	346, 604
PciSI	GCTCTTC	1 cut(s)	84
PctI	GAATGC	3 cut(s)	248, 909, 1065
PfeI	GAWTC	7 cut(s)	7, 50, 173, 307, 991, 1273, 1406
PfoI	TCCNGGA	2 cut(s)	863, 1368
PinAI	ACCGGT	1 cut(s)	1348
PleI	GAGTC	1 cut(s)	498
PpsI	GAGTC	1 cut(s)	498
Psp6I	CCWGG	4 cut(s)	104, 863, 1122, 1368
PspGI	CCWGG	4 cut(s)	104, 863, 1122, 1368
PspN4I	GGNNCC	2 cut(s)	673, 862
PspPI	GGNCC	2 cut(s)	833, 1120
PstI	CTGCAG	1 cut(s)	583
RsaI	GTAC	2 cut(s)	187, 1104
RsaNI	GTAC	2 cut(s)	186, 1103
SapI	GCTCTTC	1 cut(s)	84
SaqAI	TTAA	4 cut(s)	81, 213, 534, 794
Sau3AI	GATC	5 cut(s)	35, 259, 343, 541, 844
Sau96I	GGNCC	2 cut(s)	833, 1120
SchI	GAGTC	1 cut(s)	499
ScrFI	CCNGG	5 cut(s)	106, 643, 865, 1124, 1370
SduI	GDGCHC	2 cut(s)	406, 1345
SexAI	ACCWGGT	1 cut(s)	104
SfaNI	GCATC	6 cut(s)	308, 362, 636, 972, 982, 1378
SfcI	CTRYAG	1 cut(s)	579
SinI	GGWCC	1 cut(s)	1120
SmlI	CTYRAG	2 cut(s)	212, 503
SmoI	CTYRAG	2 cut(s)	212, 503
SsiI	CCGC	3 cut(s)	146, 614, 977
SspMI	CTAG	3 cut(s)	138, 486, 839
StyD4I	CCNGG	5 cut(s)	104, 641, 863, 1122, 1368
StyI	CCWWGG	2 cut(s)	74, 1155
TaaI	ACNGT	5 cut(s)	389, 621, 814, 915, 1178
TaiI	ACGT	4 cut(s)	187, 383, 470, 1072
TaqI	TCGA	3 cut(s)	943, 962, 1345
TaqII	GACCGA	1 cut(s)	183
TauI	GCSGC	1 cut(s)	617
TfiI	GAWTC	7 cut(s)	7, 50, 173, 307, 991, 1273, 1406
Tru1I	TTAA	4 cut(s)	81, 213, 534, 794
Tru9I	TTAA	4 cut(s)	81, 213, 534, 794
TscAI	CASTG	2 cut(s)	779, 819
TseFI	GTSAC	1 cut(s)	909
TseI	GCWGC	9 cut(s)	94, 738, 889, 919, 985, 1003, 1045, 1213, 1216
Tsp45I	GTSAC	1 cut(s)	909
TspDTI	ATGAA	6 cut(s)	102, 363, 431, 887, 1383, 1428
TspGWI	ACGGA	1 cut(s)	713
TspRI	CASTG	2 cut(s)	779, 819
Vha464I	CTTAAG	1 cut(s)	212
VneI	GTGCAC	1 cut(s)	402
VpaK11BI	GGWCC	1 cut(s)	1120
XapI	RAATTY	5 cut(s)	446, 868, 894, 1221, 1311
XspI	CTAG	3 cut(s)	138, 486, 839
ZraI	GACGTC	1 cut(s)	1070
Zsp2I	ATGCAT	2 cut(s)	301, 975

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.