Rh2DG230300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005483
Physical Location & Seq
Reverse (-)
22831780 .. 22835904
4125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG230300.1

Sequence Viewer

Length: 1443 bp
ATGCAGATTCAGTCAAAAACAGCTTTTGGTTTTGGATCAAGATATGGAGAATCTGAAATTGATAATAAGCCACCAAGGTTTAAAAATGAAGAGCTGCCTGAGACCTGGTATAATCAGGTTATTGAGAGACATAGGGCTAGCAAACCGCATAGGTTGTCATCAGCAGACCGAGAATCAGAGAAACGTACTCCAGAGGAGATGTCTGCTTATCTTAAGCTTGCTGTGAAGCATAAGAAAAGGTGTTTAGCATTCAAAGAAGATCAGTATGTGGGATATGGAAACCCCCTCCAAGAAAATGCATCACAGAATCCACATTCTGTTTTAGATGGTAGTAGTTCAATTGATCATGAAGCATCTTTTTTCCCAGAAACAATGTTTACGTTGAACTGTGTTCCTGATAGTGCACTTCCACCTATGAATAGAGTGCAAGACGACCAAAAAGTTGAATTTTATGGAGTTCTTGATACGTTACCTCAGGCTTTGACTAGGAGTCCTGTTATGCTTGAGAGGCTTGGTATAAGACCTGAATACCTTAACATGGATCAAGGAGGAAGCTTACATCGTGGAAAGAATGGGTCTGCAGGGAACAAAAGTTGTCTTACTCATCAGCAAGCAGCACAGTTATCTCAAAAGGTAATAGCCCGGATGCTGACAAATGTGGGATTTGAAGGTTCCTCAGAAGTTCCAATTGAAGTTTTCTCTCAGTTGCTAAGCTGTCATATCCGTAAATTGGGTAGCAGCTTGAAAGTTCTTACTGATAGTTACAGAAAGCAGTGTTCAGCAATTGAACTATTGAAGATGTTCCTTCAAACAGTGGGATATAGTAATTTTGGCCCTCTAGCTGATCAAGTCAAGGATGGCTCCAGGAATTTCCATCAACAAAGTCAGCAGCAAATTCATGGAATGCAGTCACAGTTGCAGCCACAGCACCAGAACCCTATTCGACTACCCCAACAAATATCGAGACAAATGCATCCGCAGATGCAGCAGATTCAGCAAATGCAGCAAATTGCCCAATCCAAGAATGTGCCATTTCAGCAACCGCAGCAGATAGAAAGAATGCGAAGACGTCAACCATCTACTCCTCGTGCTGGTATGGACTTGGTACAGGACAGACCAATGGTCCAGGTCAAGATTGAAGCTCCATCAGAGTTACCCATGGATAGTAATGCCTTCAACAGTTTCAACAACAGACATCCACAATTGCAGTTCAGGCAGCAGCAAATTCCTGCAATGTCGAATCCTACAATGCAAAATGTCCCTGCTCAGTCAGGGAATCAGTTTAGACAGATATCTCCTTCACAAATTGCACAAATTCAATCACAGAACGCCGGTGTTCTTAGGGCTCGACCAGTGAAGGTTGAAGGTTTCCAGGAACTAATGGGTGGAGATGCTTCATCTAAGCATGATTCAGATGAAAATAGGCTGACCTCCCCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromo_TP

Protein Analysis

480

Amino Acids

54.39

Weight (kDa)

9.23

Isoelectric Point (pI)

65.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1072
AciI CCGC 3 cut(s) 146, 977, 1043
AclWI GGATC 2 cut(s) 43, 549
AcsI RAATTY 5 cut(s) 446, 868, 894, 1224, 1314
AcyI GRCGYC 1 cut(s) 1069
AfaI GTAC 2 cut(s) 187, 1107
AfiI CCNNNNNNNGG 3 cut(s) 538, 730, 1091
AflII CTTAAG 1 cut(s) 212
AhdI GACNNNNNGTC 2 cut(s) 489, 1121
AjnI CCWGG 4 cut(s) 104, 863, 1125, 1371
AluBI AGCT 8 cut(s) 23, 94, 217, 555, 714, 741, 842, 1142
AluI AGCT 8 cut(s) 23, 94, 217, 555, 714, 741, 842, 1142
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 3 cut(s) 95, 121, 958
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 2 cut(s) 43, 549
AoxI GGCC 1 cut(s) 832
ApaLI GTGCAC 1 cut(s) 402
ApoI RAATTY 5 cut(s) 446, 868, 894, 1224, 1314
Asp700I GAANNNNTTC 1 cut(s) 800
AspS9I GGNCC 2 cut(s) 833, 1123
AsuC2I CCSGG 1 cut(s) 643
AsuNHI GCTAGC 1 cut(s) 137
AvaII GGWCC 1 cut(s) 1123
AxyI CCTNAGG 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 1348
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 1 cut(s) 1072
Bbv12I GWGCWC 1 cut(s) 406
BccI CCATC 5 cut(s) 320, 851, 882, 1084, 1153
BcgI CGANNNNNNTGC 2 cut(s) 952, 986
BciT130I CCWGG 4 cut(s) 106, 865, 1127, 1373
BclI TGATCA 2 cut(s) 343, 844
BcnI CCSGG 1 cut(s) 643
BcoDI GTCTC 3 cut(s) 95, 121, 958
BfaI CTAG 3 cut(s) 138, 486, 839
BfmI CTRYAG 1 cut(s) 579
BfrI CTTAAG 1 cut(s) 212
BlpI GCTNAGC 1 cut(s) 710
Bme1390I CCNGG 5 cut(s) 106, 643, 865, 1127, 1373
Bme18I GGWCC 1 cut(s) 1123
BmeRI GACNNNNNGTC 2 cut(s) 489, 1121
BmgT120I GGNCC 2 cut(s) 833, 1123
BmiI GGNNCC 2 cut(s) 673, 862
BmrFI CCNGG 5 cut(s) 106, 643, 865, 1127, 1373
BmsI GCATC 6 cut(s) 308, 362, 636, 972, 982, 1381
BmtI GCTAGC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 1072
BpmI CTGGAG 2 cut(s) 174, 847
Bpu1102I GCTNAGC 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 643
BsaHI GRCGYC 1 cut(s) 1069
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 74, 1158
Bsc4I CCNNNNNNNGG 3 cut(s) 538, 730, 1091
Bse118I RCCGGY 1 cut(s) 1331
Bse1I ACTGG 1 cut(s) 1352
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 1239
BseBI CCWGG 4 cut(s) 106, 865, 1127, 1373
BseDI CCNNGG 2 cut(s) 74, 1158
BseGI GGATG 4 cut(s) 651, 862, 973, 1195
BseLI CCNNNNNNNGG 3 cut(s) 538, 730, 1091
BseMI GCAATG 1 cut(s) 1239
BseMII CTCAG 5 cut(s) 90, 488, 690, 716, 1280
BseNI ACTGG 1 cut(s) 1352
BseRI GAGGAG 2 cut(s) 209, 1074
BseSI GKGCMC 1 cut(s) 406
BshFI GGCC 1 cut(s) 834
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 2 cut(s) 643, 1332
BslFI GGGAC 1 cut(s) 1244
BslI CCNNNNNNNGG 3 cut(s) 538, 730, 1091
BsmAI GTCTC 3 cut(s) 95, 121, 958
BsmFI GGGAC 1 cut(s) 1244
BsmI GAATGC 3 cut(s) 248, 909, 1065
BsnI GGCC 1 cut(s) 834
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 406, 1348
Bsp143I GATC 5 cut(s) 35, 259, 343, 541, 844
Bsp1720I GCTNAGC 1 cut(s) 710
Bsp19I CCATGG 1 cut(s) 1158
BspACI CCGC 3 cut(s) 146, 977, 1043
BspANI GGCC 1 cut(s) 834
BspCNI CTCAG 5 cut(s) 91, 487, 689, 715, 1279
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 2 cut(s) 673, 862
BspMAI CTGCAG 1 cut(s) 583
BspOI GCTAGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 43, 549
BspQI GCTCTTC 1 cut(s) 84
BspTI CTTAAG 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 1239
BsrFI RCCGGY 1 cut(s) 1331
BsrI ACTGG 1 cut(s) 1352
BssAI RCCGGY 1 cut(s) 1331
BssECI CCNNGG 2 cut(s) 74, 1158
BssMI GATC 5 cut(s) 35, 259, 343, 541, 844
BssNI GRCGYC 1 cut(s) 1069
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 2 cut(s) 74, 1158
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 4 cut(s) 106, 865, 1127, 1373
Bst4CI ACNGT 5 cut(s) 389, 621, 814, 915, 1181
Bst6I CTCTTC 1 cut(s) 84
BstACI GRCGYC 1 cut(s) 1069
BstAFI CTTAAG 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 139, 219, 612
BstDEI CTNAG 8 cut(s) 99, 474, 676, 702, 710, 1266, 1340, 1401
BstDSI CCRYGG 1 cut(s) 1158
BstF5I GGATG 4 cut(s) 651, 862, 973, 1195
BstKTI GATC 5 cut(s) 38, 262, 346, 544, 847
BstMAI GTCTC 3 cut(s) 95, 121, 958
BstMBI GATC 5 cut(s) 35, 259, 343, 541, 844
BstMWI GCNNNNNNNGC 8 cut(s) 508, 925, 985, 994, 1003, 1036, 1045, 1213
BstNI CCWGG 4 cut(s) 106, 865, 1127, 1373
BstSCI CCNGG 5 cut(s) 104, 641, 863, 1125, 1371
BstSFI CTRYAG 1 cut(s) 579
BstSLI GKGCMC 1 cut(s) 406
BstV2I GAAGAC 1 cut(s) 1072
Bsu36I CCTNAGG 1 cut(s) 474
BsuRI GGCC 1 cut(s) 834
BtgI CCRYGG 1 cut(s) 1158
BtsCI GGATG 4 cut(s) 651, 862, 973, 1195
BtsI GCAGTG 1 cut(s) 779
BtsIMutI CAGTG 3 cut(s) 779, 819, 1359
Cac8I GCNNGC 3 cut(s) 139, 219, 612
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 1 cut(s) 1331
Cfr13I GGNCC 2 cut(s) 833, 1123
CsiI ACCWGGT 1 cut(s) 104
Csp6I GTAC 2 cut(s) 186, 1106
CviAII CATG 5 cut(s) 347, 538, 899, 1159, 1406
CviQI GTAC 2 cut(s) 186, 1106
DdeI CTNAG 8 cut(s) 99, 474, 676, 702, 710, 1266, 1340, 1401
DpnI GATC 5 cut(s) 37, 261, 345, 543, 846
DpnII GATC 5 cut(s) 35, 259, 343, 541, 844
DraI TTTAAA 1 cut(s) 82
DriI GACNNNNNGTC 2 cut(s) 489, 1121
Eam1104I CTCTTC 1 cut(s) 84
Eam1105I GACNNNNNGTC 2 cut(s) 489, 1121
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 74, 1158
Eco24I GRGCYC 1 cut(s) 1348
Eco31I GGTCTC 1 cut(s) 95
Eco32I GATATC 1 cut(s) 1293
Eco47I GGWCC 1 cut(s) 1123
Eco81I CCTNAGG 1 cut(s) 474
EcoRII CCWGG 4 cut(s) 104, 863, 1125, 1371
EcoRV GATATC 1 cut(s) 1293
EcoT14I CCWWGG 2 cut(s) 74, 1158
EcoT22I ATGCAT 2 cut(s) 301, 975
EcoT38I GRGCYC 1 cut(s) 1348
ErhI CCWWGG 2 cut(s) 74, 1158
FaeI CATG 5 cut(s) 350, 541, 902, 1162, 1409
FaqI GGGAC 1 cut(s) 1244
FatI CATG 5 cut(s) 346, 537, 898, 1158, 1405
FbaI TGATCA 2 cut(s) 343, 844
FokI GGATG 4 cut(s) 658, 869, 960, 1182
FriOI GRGCYC 1 cut(s) 1348
FspBI CTAG 3 cut(s) 138, 486, 839
GsuI CTGGAG 2 cut(s) 174, 847
HaeIII GGCC 1 cut(s) 834
HapII CCGG 2 cut(s) 643, 1332
Hin1I GRCGYC 1 cut(s) 1069
Hin1II CATG 5 cut(s) 350, 541, 902, 1162, 1409
HincII GTYRAC 1 cut(s) 1073
HindII GTYRAC 1 cut(s) 1073
HindIII AAGCTT 2 cut(s) 215, 553
HinfI GANTC 9 cut(s) 7, 50, 173, 307, 490, 991, 1240, 1276, 1409
HpaII CCGG 2 cut(s) 643, 1332
Hpy166II GTNNAC 3 cut(s) 378, 404, 1073
Hpy188I TCNGA 5 cut(s) 55, 178, 679, 1150, 1414
Hpy188III TCNNGA 7 cut(s) 39, 191, 347, 395, 461, 963, 1132
Hpy8I GTNNAC 3 cut(s) 378, 404, 1073
HpyAV CCTTC 6 cut(s) 662, 815, 1183, 1308, 1351, 1358
HpyCH4III ACNGT 5 cut(s) 389, 621, 814, 915, 1181
HpyCH4IV ACGT 4 cut(s) 184, 380, 467, 1069
HpyF10VI GCNNNNNNNGC 8 cut(s) 508, 925, 985, 994, 1003, 1036, 1045, 1213
HpyF3I CTNAG 8 cut(s) 99, 474, 676, 702, 710, 1266, 1340, 1401
HpySE526I ACGT 4 cut(s) 184, 380, 467, 1069
Hsp92I GRCGYC 1 cut(s) 1069
Hsp92II CATG 5 cut(s) 350, 541, 902, 1162, 1409
Ksp22I TGATCA 2 cut(s) 343, 844
Kzo9I GATC 5 cut(s) 35, 259, 343, 541, 844
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 2 cut(s) 866, 1147
LweI GCATC 6 cut(s) 308, 362, 636, 972, 982, 1381
MabI ACCWGGT 1 cut(s) 104
MaeI CTAG 3 cut(s) 138, 486, 839
MaeII ACGT 4 cut(s) 184, 380, 467, 1069
MaeIII GTNAC 4 cut(s) 468, 761, 909, 1152
MalI GATC 5 cut(s) 37, 261, 345, 543, 846
MboI GATC 5 cut(s) 35, 259, 343, 541, 844
MboII GAAGA 4 cut(s) 101, 269, 808, 1077
MfeI CAATTG 4 cut(s) 339, 687, 783, 1202
MhlI GDGCHC 2 cut(s) 406, 1348
MlyI GAGTC 1 cut(s) 499
MnlI CCTC 9 cut(s) 187, 296, 483, 501, 542, 685, 846, 1095, 1441
Mph1103I ATGCAT 2 cut(s) 301, 975
MroXI GAANNNNTTC 1 cut(s) 800
MseI TTAA 3 cut(s) 81, 213, 534
MspCI CTTAAG 1 cut(s) 212
MspI CCGG 2 cut(s) 643, 1332
MspR9I CCNGG 5 cut(s) 106, 643, 865, 1127, 1373
MunI CAATTG 4 cut(s) 339, 687, 783, 1202
Mva1269I GAATGC 3 cut(s) 248, 909, 1065
MvaI CCWGG 4 cut(s) 106, 865, 1127, 1373
MwoI GCNNNNNNNGC 8 cut(s) 508, 925, 985, 994, 1003, 1036, 1045, 1213
NciI CCSGG 1 cut(s) 643
NcoI CCATGG 1 cut(s) 1158
NdeII GATC 5 cut(s) 35, 259, 343, 541, 844
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 5 cut(s) 350, 541, 902, 1162, 1409
NlaIV GGNNCC 2 cut(s) 673, 862
NmuCI GTSAC 1 cut(s) 909
NsiI ATGCAT 2 cut(s) 301, 975
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 84
PctI GAATGC 3 cut(s) 248, 909, 1065
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 8 cut(s) 7, 50, 173, 307, 991, 1240, 1276, 1409
PfoI TCCNGGA 2 cut(s) 863, 1371
PleI GAGTC 1 cut(s) 498
PpsI GAGTC 1 cut(s) 498
Psp6I CCWGG 4 cut(s) 104, 863, 1125, 1371
PspGI CCWGG 4 cut(s) 104, 863, 1125, 1371
PspN4I GGNNCC 2 cut(s) 673, 862
PspPI GGNCC 2 cut(s) 833, 1123
PstI CTGCAG 1 cut(s) 583
RsaI GTAC 2 cut(s) 187, 1107
RsaNI GTAC 2 cut(s) 186, 1106
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 3 cut(s) 81, 213, 534
Sau3AI GATC 5 cut(s) 35, 259, 343, 541, 844
Sau96I GGNCC 2 cut(s) 833, 1123
SchI GAGTC 1 cut(s) 499
ScrFI CCNGG 5 cut(s) 106, 643, 865, 1127, 1373
SduI GDGCHC 2 cut(s) 406, 1348
SexAI ACCWGGT 1 cut(s) 104
SfaNI GCATC 6 cut(s) 308, 362, 636, 972, 982, 1381
SfcI CTRYAG 1 cut(s) 579
SgrAI CRCCGGYG 1 cut(s) 1331
SinI GGWCC 1 cut(s) 1123
SmlI CTYRAG 2 cut(s) 212, 503
SmoI CTYRAG 2 cut(s) 212, 503
SsiI CCGC 3 cut(s) 146, 977, 1043
SspMI CTAG 3 cut(s) 138, 486, 839
StyD4I CCNGG 5 cut(s) 104, 641, 863, 1125, 1371
StyI CCWWGG 2 cut(s) 74, 1158
TaaI ACNGT 5 cut(s) 389, 621, 814, 915, 1181
TaiI ACGT 4 cut(s) 187, 383, 470, 1072
TaqI TCGA 4 cut(s) 943, 962, 1238, 1348
TaqII GACCGA 1 cut(s) 183
TfiI GAWTC 8 cut(s) 7, 50, 173, 307, 991, 1240, 1276, 1409
Tru1I TTAA 3 cut(s) 81, 213, 534
Tru9I TTAA 3 cut(s) 81, 213, 534
TscAI CASTG 3 cut(s) 779, 819, 1359
TseFI GTSAC 1 cut(s) 909
Tsp45I GTSAC 1 cut(s) 909
TspDTI ATGAA 6 cut(s) 102, 363, 431, 887, 1386, 1431
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 3 cut(s) 779, 819, 1359
Vha464I CTTAAG 1 cut(s) 212
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 1123
XapI RAATTY 5 cut(s) 446, 868, 894, 1224, 1314
XmnI GAANNNNTTC 1 cut(s) 800
XspI CTAG 3 cut(s) 138, 486, 839
ZraI GACGTC 1 cut(s) 1070
Zsp2I ATGCAT 2 cut(s) 301, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.