MD01G1051100.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005803
Physical Location & Seq
Forward (+)
15473114 .. 15477578
4465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1051100.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGTTCCTTGTATGGATTTCTTGAACTGGCTTGAACCTGACATGTCCTTCAAGGTCCTAACTTGTTTGGATGATCCATCTGATCTTTTTCGCGTTGGTTCTGTCTCACGCTCTTGGCGTCATTTTGTGATAGAACATGGTCTTTGTAAAAAGCTGTGCTTGAGAATGTTCCCTCAGTTATCTAGAGTTGCTGGTGTGGTTGAGTTAAACAGCCCCGACGCAGAAGAGTTTGCAGAAGTTGGATCTAGCAATTCAAAGGAATTGGAAACCTTGGAAAGGGAGCATAGGGTCTATGCTTTTTTATCTCGTGGTTTTATATCTTTTGATGTTAGGAGTTCTATTTCCAATGCAATCAGTGCTTCCAGCACTGACAATCATCCGGAAGAAAGCATTCACAATACTCTACAACCAAGAGACCTGGTCGGACGGAGAGCTTCGTATTGGTCGAGTAAAGGCCAAAGCGACCCCGCAGTGCCTGAGTTTCTGATATACAAGTTGGTGTCTGATTTGGTTGTAATTAATGAAATCAGCATACATCCTTTCCAAGCTTTTTTCCAGGCAGGTTTCCCAATATATTCAACCAATGCTGTGCGATTTCGAATGGGTCATGTCAAATCCCCTGTGGTTGTAGAGGTTGATTCAAAGGGTGACTCATGTCATGAAAACAAGTTTGTATGGACCTACACTTCAGAAGTGTTCCGAATGACTCAGGAGAACCGCTTGCAGACATTTAAGCTTCCAGAACCAGTTCTTTGCATAGGTGGAATTCTGCAGATTGAGCTTCTGGGTAGAGTTCAGAGACAAGAAATGGACGGATTGTTCTATATATGTGTGTGTCATGTTGAAGTCAAAGGGAAGCCATTATCGCCTGCATTTGGTGTTGAAATTCATGAACCATCTGGGGAGTTTGTGTTGAAGAACGATGTGCAGGCCAAGTTGCACAACCAACCCGTCGAGAATGAGTCCGTTGTTATCTCCACTGTTAATTTGGAGAATATGAGAGACGACATGTTGCCAGTTGTGAATTTGAATGTAGAAGGTATTGCGGAGGAATATGAATGGGAGGAAGAGGAGGGATCTGATGAAGAGTATTTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

367

Amino Acids

41.62

Weight (kDa)

4.87

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 15 - 55 9.6e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 551
AccII CGCG 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 379
AciI CCGC 3 cut(s) 468, 718, 1046
AclWI GGATC 3 cut(s) 68, 250, 1084
AcsI RAATTY 3 cut(s) 765, 885, 1024
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 1 cut(s) 118
AfiI CCNNNNNNNGG 2 cut(s) 276, 875
AflIII ACRYGT 2 cut(s) 42, 1008
AjnI CCWGG 2 cut(s) 417, 555
AloI GAACNNNNNNTCC 2 cut(s) 803, 835
AluBI AGCT 5 cut(s) 154, 434, 548, 736, 781
AluI AGCT 5 cut(s) 154, 434, 548, 736, 781
Alw26I GTCTC 4 cut(s) 109, 408, 793, 996
AlwI GGATC 3 cut(s) 68, 250, 1084
AlwNI CAGNNNCTG 1 cut(s) 476
Aor13HI TCCGGA 1 cut(s) 379
AoxI GGCC 2 cut(s) 454, 930
ApoI RAATTY 3 cut(s) 765, 885, 1024
AseI ATTAAT 1 cut(s) 519
Asp700I GAANNNNTTC 2 cut(s) 390, 747
AspS9I GGNCC 2 cut(s) 55, 678
AsuHPI GGTGA 1 cut(s) 659
AsuII TTCGAA 1 cut(s) 598
AvaII GGWCC 2 cut(s) 55, 678
BauI CACGAG 1 cut(s) 306
BccI CCATC 2 cut(s) 85, 904
BciT130I CCWGG 2 cut(s) 419, 557
BcoDI GTCTC 4 cut(s) 109, 408, 793, 996
BfaI CTAG 2 cut(s) 183, 246
BfmI CTRYAG 1 cut(s) 770
BfuAI ACCTGC 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 419, 557
Bme18I GGWCC 2 cut(s) 55, 678
BmgT120I GGNCC 2 cut(s) 55, 678
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 419, 557
BoxI GACNNNNGTC 1 cut(s) 654
Bpu14I TTCGAA 1 cut(s) 598
BpuEI CTTGAG 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 118
BsaI GGTCTC 1 cut(s) 408
BsaJI CCNNGG 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 896, 926
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 875
Bse1I ACTGG 3 cut(s) 32, 746, 1016
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 2 cut(s) 419, 557
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 3 cut(s) 76, 376, 535
BseLI CCNNNNNNNGG 2 cut(s) 276, 875
BseMII CTCAG 3 cut(s) 188, 468, 722
BseNI ACTGG 3 cut(s) 32, 746, 1016
BseRI GAGGAG 1 cut(s) 1085
BsgI GTGCAG 1 cut(s) 947
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 456, 932
BsiSI CCGG 1 cut(s) 380
BslI CCNNNNNNNGG 2 cut(s) 276, 875
BsmAI GTCTC 4 cut(s) 109, 408, 793, 996
BsmBI CGTCTC 1 cut(s) 996
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 2 cut(s) 456, 932
Bso31I GGTCTC 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 598
Bsp13I TCCGGA 1 cut(s) 379
Bsp143I GATC 4 cut(s) 73, 82, 242, 1076
BspACI CCGC 3 cut(s) 468, 718, 1046
BspANI GGCC 2 cut(s) 456, 932
BspCNI CTCAG 3 cut(s) 187, 469, 721
BspEI TCCGGA 1 cut(s) 379
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 2 cut(s) 658, 889
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 774
BspMI ACCTGC 1 cut(s) 551
BspPI GGATC 3 cut(s) 68, 250, 1084
BspT104I TTCGAA 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 408
BsrI ACTGG 3 cut(s) 32, 746, 1016
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 4 cut(s) 73, 82, 242, 1076
BssNI GRCGYC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 270
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 419, 557
Bst4CI ACNGT 1 cut(s) 982
Bst6I CTCTTC 3 cut(s) 219, 1062, 1080
BstACI GRCGYC 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 598
BstC8I GCNNGC 3 cut(s) 722, 870, 930
BstDEI CTNAG 3 cut(s) 174, 477, 708
BstENI CCTNNNNNAGG 1 cut(s) 274
BstF5I GGATG 3 cut(s) 76, 376, 535
BstFNI CGCG 1 cut(s) 93
BstKTI GATC 4 cut(s) 76, 85, 245, 1079
BstMAI GTCTC 4 cut(s) 109, 408, 793, 996
BstMBI GATC 4 cut(s) 73, 82, 242, 1076
BstMWI GCNNNNNNNGC 3 cut(s) 356, 778, 865
BstNI CCWGG 2 cut(s) 419, 557
BstNSI RCATGY 2 cut(s) 46, 1012
BstPAI GACNNNNGTC 1 cut(s) 654
BstSCI CCNGG 2 cut(s) 417, 555
BstSFI CTRYAG 1 cut(s) 770
BstUI CGCG 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 242, 1076
BstYI RGATCY 2 cut(s) 242, 1076
BsuRI GGCC 2 cut(s) 456, 932
BtsCI GGATG 3 cut(s) 76, 376, 535
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 4 cut(s) 361, 366, 477, 978
BveI ACCTGC 1 cut(s) 551
Cac8I GCNNGC 3 cut(s) 722, 870, 930
CaiI CAGNNNCTG 1 cut(s) 476
CciI TCATGA 2 cut(s) 658, 889
Cfr13I GGNCC 2 cut(s) 55, 678
CseI GACGC 2 cut(s) 107, 227
CsiI ACCWGGT 1 cut(s) 417
CviAII CATG 8 cut(s) 43, 137, 608, 654, 659, 839, 890, 1009
DdeI CTNAG 3 cut(s) 174, 477, 708
DpnI GATC 4 cut(s) 75, 84, 244, 1078
DpnII GATC 4 cut(s) 73, 82, 242, 1076
Eam1104I CTCTTC 3 cut(s) 219, 1062, 1080
EarI CTCTTC 3 cut(s) 219, 1062, 1080
Eco130I CCWWGG 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 408
Eco47I GGWCC 2 cut(s) 55, 678
Eco57I CTGAAG 1 cut(s) 672
EcoNI CCTNNNNNAGG 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 2 cut(s) 417, 555
EcoT14I CCWWGG 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 270
Esp3I CGTCTC 1 cut(s) 996
FaeI CATG 8 cut(s) 46, 140, 611, 657, 662, 842, 893, 1012
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FatI CATG 8 cut(s) 42, 136, 607, 653, 658, 838, 889, 1008
FauI CCCGC 1 cut(s) 475
FokI GGATG 3 cut(s) 83, 363, 522
FspBI CTAG 2 cut(s) 183, 246
HaeIII GGCC 2 cut(s) 456, 932
HapII CCGG 1 cut(s) 380
HgaI GACGC 2 cut(s) 107, 227
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 8 cut(s) 46, 140, 611, 657, 662, 842, 893, 1012
HindIII AAGCTT 2 cut(s) 546, 734
HinfI GANTC 4 cut(s) 638, 650, 706, 962
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 659
Hpy188I TCNGA 8 cut(s) 82, 425, 486, 505, 691, 701, 798, 1081
Hpy188III TCNNGA 8 cut(s) 22, 183, 380, 659, 710, 740, 890, 955
Hpy99I CGWCG 2 cut(s) 221, 956
HpyAV CCTTC 2 cut(s) 58, 1031
HpyCH4III ACNGT 1 cut(s) 982
HpyCH4V TGCA 8 cut(s) 233, 350, 724, 756, 772, 872, 928, 940
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 778, 865
HpyF3I CTNAG 3 cut(s) 174, 477, 708
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 8 cut(s) 46, 140, 611, 657, 662, 842, 893, 1012
Kpn2I TCCGGA 1 cut(s) 379
Kzo9I GATC 4 cut(s) 73, 82, 242, 1076
LmnI GCTCC 1 cut(s) 280
MabI ACCWGGT 1 cut(s) 417
MaeI CTAG 2 cut(s) 183, 246
MaeIII GTNAC 1 cut(s) 647
MalI GATC 4 cut(s) 75, 84, 244, 1078
MboI GATC 4 cut(s) 73, 82, 242, 1076
MboII GAAGA 5 cut(s) 236, 395, 928, 1079, 1097
MflI RGATCY 2 cut(s) 242, 1076
MluCI AATT 7 cut(s) 250, 260, 516, 765, 885, 985, 1024
MlyI GAGTC 3 cut(s) 644, 700, 971
MmeI TCCRAC 2 cut(s) 220, 403
MnlI CCTC 6 cut(s) 183, 625, 1042, 1057, 1063, 1066
MroI TCCGGA 1 cut(s) 379
MroXI GAANNNNTTC 2 cut(s) 390, 747
MseI TTAA 4 cut(s) 206, 519, 732, 984
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 2 cut(s) 419, 557
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 2 cut(s) 419, 557
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 356, 778, 865
NdeII GATC 4 cut(s) 73, 82, 242, 1076
NlaIII CATG 8 cut(s) 46, 140, 611, 657, 662, 842, 893, 1012
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 647
NspI RCATGY 2 cut(s) 46, 1012
NspV TTCGAA 1 cut(s) 598
PagI TCATGA 2 cut(s) 658, 889
PciI ACATGT 2 cut(s) 42, 1008
PcsI WCGNNNNNNNCGW 2 cut(s) 115, 443
PctI GAATGC 1 cut(s) 390
PdmI GAANNNNTTC 2 cut(s) 390, 747
PfeI GAWTC 1 cut(s) 638
PflFI GACNNNGTC 1 cut(s) 419
PleI GAGTC 3 cut(s) 644, 700, 970
PpsI GAGTC 3 cut(s) 644, 700, 970
PpuMI RGGWCCY 1 cut(s) 55
PscI ACATGT 2 cut(s) 42, 1008
PshAI GACNNNNGTC 1 cut(s) 654
PshBI ATTAAT 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 417, 555
PspGI CCWGG 2 cut(s) 417, 555
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 55, 678
PspPPI RGGWCCY 1 cut(s) 55
PstI CTGCAG 1 cut(s) 774
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 2 cut(s) 242, 1076
PsyI GACNNNGTC 1 cut(s) 419
SaqAI TTAA 4 cut(s) 206, 519, 732, 984
Sau3AI GATC 4 cut(s) 73, 82, 242, 1076
Sau96I GGNCC 2 cut(s) 55, 678
SchI GAGTC 3 cut(s) 644, 700, 971
ScrFI CCNGG 2 cut(s) 419, 557
SexAI ACCWGGT 1 cut(s) 417
SfcI CTRYAG 1 cut(s) 770
SfuI TTCGAA 1 cut(s) 598
SinI GGWCC 2 cut(s) 55, 678
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 7 cut(s) 250, 260, 516, 765, 885, 985, 1024
SsiI CCGC 3 cut(s) 468, 718, 1046
SspMI CTAG 2 cut(s) 183, 246
StyD4I CCNGG 2 cut(s) 417, 555
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 982
TaqI TCGA 3 cut(s) 446, 598, 954
TasI AATT 7 cut(s) 250, 260, 516, 765, 885, 985, 1024
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 4 cut(s) 206, 519, 732, 984
Tru9I TTAA 4 cut(s) 206, 519, 732, 984
TscAI CASTG 4 cut(s) 361, 373, 477, 985
TseFI GTSAC 1 cut(s) 647
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 6 cut(s) 537, 675, 878, 906, 1071, 1098
TspGWI ACGGA 3 cut(s) 442, 828, 955
TspRI CASTG 4 cut(s) 361, 373, 477, 985
Tth111I GACNNNGTC 1 cut(s) 419
VpaK11BI GGWCC 2 cut(s) 55, 678
VspI ATTAAT 1 cut(s) 519
XagI CCTNNNNNAGG 1 cut(s) 274
XapI RAATTY 3 cut(s) 765, 885, 1024
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 2 cut(s) 46, 1012
XcmI CCANNNNNNNNNTGG 1 cut(s) 985
XmnI GAANNNNTTC 2 cut(s) 390, 747
XspI CTAG 2 cut(s) 183, 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.