MD07G1128000.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005803
Physical Location & Seq
Forward (+)
18197798 .. 18202196
4399 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1128000.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGAGTCCTTGTATGGATTTCTTGAACTGGCTTGAACCTGACATGTCCTTAAATGTCCTAACTTGTTTGGACGATCCATCTGATCTTGTTCGTGTTAGTTCTGTCTCACGCTCTTGGCGTCATTTTGTGATAGAAAATGATCTTTGTAAAAAGCTGTGCTTGAGAATGTTTCCTCAGTTATCTAGAGTTGCTGGTGTGGTTGAGTTAAACAGCCCTGACGCAGAAGAGGTTGCAGAAATTGGATCTAGCAAGTCAAAGGAATTGGAAACCTTGGAAAGGGAGCATAGGGTCTATGCTTTTTTAGCTCGTGGTTTTATATCTTTCAATGTGAGGAGTTCTATTTCTAAGGCAATCAGTGCTTCCAGCACCGACAATTATCCGGAAGAAAGCATCCGCAATACTCTAGAACCAAGAGTCATGGTGGGACAGAGAGCTTCGTATTGGTCAAGTAAAGGCCAAAGTGACCCTGCAGTGCCTGAGATTCTGATATACAAGTTGGTGTCTGATTTAGTTGTAATTAATGAAGTCAGCATACATCCTTTCCAAGCTTTTTTCCAGGTAGGTTTACCCATATATTCAGCCAAGGCTGTGCGATTTCATATGGGTTGTGCCAAATCCCTTGTGGTTGTAGAGTGTGATTCAAAGGATGAATCATGTCATGAGAACAAGTTCGTATGGACCTACACTTCAGAAGTGTTCCCAATGACTCAGGAGAACCGCCTGCAGACATTTAAGCTTCCAGAACCAGTTCTTTGCATAGGTGGAATTTTGCAGATTGAGCTTCTGGGTAGAGTACAGAGACAAGAAATGGATGGCTTGTTCTATATATGTGTGTCTCATGTTGAAGTCAAAGGGAAGCCATTATCACCTGCATTTGGCGTTGAAATTCATGTCCCATCTGGGGAGTTTGTGTTGAAGAACGATGTGCAGGCCAAGTTGCACAACCAACAGAACTTGCCCGAGAACGAGTCCATTATTATCTCCACAGTTAATTTGGAGGATATGAGAGGCATGCAGCCATTTGTGAATTGGAATGTAGTAGGTATTGAGGAGGAATACCAAGGGGAGGAAGAGGACGGATCTGATGAAGAATATTTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

368

Amino Acids

41.54

Weight (kDa)

4.82

Isoelectric Point (pI)

57.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 14 - 55 4.6e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 877
Acc36I ACCTGC 1 cut(s) 877
AccIII TCCGGA 1 cut(s) 379
AciI CCGC 2 cut(s) 394, 718
AclWI GGATC 3 cut(s) 68, 250, 1087
AcsI RAATTY 2 cut(s) 765, 885
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 1 cut(s) 795
AfiI CCNNNNNNNGG 4 cut(s) 276, 875, 901, 1066
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 7 cut(s) 25, 35, 325, 642, 845, 884, 916
AjnI CCWGG 1 cut(s) 555
AloI GAACNNNNNNTCC 2 cut(s) 803, 835
AluBI AGCT 6 cut(s) 154, 305, 434, 548, 736, 781
AluI AGCT 6 cut(s) 154, 305, 434, 548, 736, 781
Alw26I GTCTC 3 cut(s) 109, 793, 840
AlwI GGATC 3 cut(s) 68, 250, 1087
AlwNI CAGNNNCTG 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 959
Aor13HI TCCGGA 1 cut(s) 379
AoxI GGCC 2 cut(s) 454, 930
ApeKI GCWGC 1 cut(s) 1015
ApoI RAATTY 2 cut(s) 765, 885
AseI ATTAAT 1 cut(s) 519
Asp700I GAANNNNTTC 2 cut(s) 668, 747
AspS9I GGNCC 1 cut(s) 678
AsuHPI GGTGA 1 cut(s) 858
AvaI CYCGRG 1 cut(s) 959
AvaII GGWCC 1 cut(s) 678
BarI GAAGNNNNNNTAC 2 cut(s) 516, 548
BauI CACGAG 1 cut(s) 306
BbvI GCAGC 1 cut(s) 1027
BccI CCATC 3 cut(s) 85, 806, 904
BciT130I CCWGG 1 cut(s) 557
BcoDI GTCTC 3 cut(s) 109, 793, 840
BfaI CTAG 3 cut(s) 183, 246, 404
BfmI CTRYAG 2 cut(s) 468, 722
BfuAI ACCTGC 1 cut(s) 877
BisI GCNGC 1 cut(s) 1016
BlsI GCNGC 1 cut(s) 1017
Bme1390I CCNGG 1 cut(s) 557
Bme18I GGWCC 1 cut(s) 678
BmeT110I CYCGRG 1 cut(s) 959
BmgT120I GGNCC 1 cut(s) 678
BmrFI CCNGG 1 cut(s) 557
BmsI GCATC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 270, 582, 1060
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 896, 926
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 875, 901, 1066
Bse1I ACTGG 2 cut(s) 32, 746
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 3 cut(s) 270, 582, 1060
BseGI GGATG 4 cut(s) 390, 535, 652, 817
BseLI CCNNNNNNNGG 4 cut(s) 276, 875, 901, 1066
BseMII CTCAG 3 cut(s) 188, 468, 722
BseNI ACTGG 2 cut(s) 32, 746
BseRI GAGGAG 2 cut(s) 346, 1064
BseXI GCAGC 1 cut(s) 1027
BsgI GTGCAG 1 cut(s) 947
BshFI GGCC 2 cut(s) 456, 932
BsiHKCI CYCGRG 1 cut(s) 959
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 438, 878
BslI CCNNNNNNNGG 4 cut(s) 276, 875, 901, 1066
BsmAI GTCTC 3 cut(s) 109, 793, 840
BsmFI GGGAC 2 cut(s) 438, 878
BsnI GGCC 2 cut(s) 456, 932
BsoBI CYCGRG 1 cut(s) 959
Bsp13I TCCGGA 1 cut(s) 379
Bsp143I GATC 5 cut(s) 73, 82, 139, 242, 1079
BspACI CCGC 2 cut(s) 394, 718
BspANI GGCC 2 cut(s) 456, 932
BspCNI CTCAG 3 cut(s) 187, 469, 721
BspEI TCCGGA 1 cut(s) 379
BspHI TCATGA 1 cut(s) 658
BspMAI CTGCAG 2 cut(s) 472, 726
BspMI ACCTGC 1 cut(s) 877
BspPI GGATC 3 cut(s) 68, 250, 1087
BsrI ACTGG 2 cut(s) 32, 746
BssECI CCNNGG 3 cut(s) 270, 582, 1060
BssMI GATC 5 cut(s) 73, 82, 139, 242, 1079
BssNI GRCGYC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 3 cut(s) 270, 582, 1060
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 988
Bst6I CTCTTC 2 cut(s) 219, 1065
BstACI GRCGYC 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 3 cut(s) 722, 930, 1013
BstDEI CTNAG 4 cut(s) 174, 345, 477, 708
BstENI CCTNNNNNAGG 1 cut(s) 274
BstF5I GGATG 4 cut(s) 390, 535, 652, 817
BstKTI GATC 5 cut(s) 76, 85, 142, 245, 1082
BstMAI GTCTC 3 cut(s) 109, 793, 840
BstMBI GATC 5 cut(s) 73, 82, 139, 242, 1079
BstMWI GCNNNNNNNGC 3 cut(s) 302, 356, 778
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 2 cut(s) 46, 1015
BstSCI CCNGG 1 cut(s) 555
BstSFI CTRYAG 2 cut(s) 468, 722
BstV1I GCAGC 1 cut(s) 1027
BstX2I RGATCY 2 cut(s) 242, 1079
BstYI RGATCY 2 cut(s) 242, 1079
BsuRI GGCC 2 cut(s) 456, 932
BtsCI GGATG 4 cut(s) 390, 535, 652, 817
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 2 cut(s) 361, 477
BveI ACCTGC 1 cut(s) 877
Cac8I GCNNGC 3 cut(s) 722, 930, 1013
CaiI CAGNNNCTG 1 cut(s) 476
CciI TCATGA 1 cut(s) 658
Cfr13I GGNCC 1 cut(s) 678
CseI GACGC 2 cut(s) 107, 227
Csp6I GTAC 1 cut(s) 794
CviAII CATG 7 cut(s) 43, 418, 654, 659, 839, 890, 1012
CviQI GTAC 1 cut(s) 794
DdeI CTNAG 4 cut(s) 174, 345, 477, 708
DpnI GATC 5 cut(s) 75, 84, 141, 244, 1081
DpnII GATC 5 cut(s) 73, 82, 139, 242, 1079
Eam1104I CTCTTC 2 cut(s) 219, 1065
EarI CTCTTC 2 cut(s) 219, 1065
Eco130I CCWWGG 3 cut(s) 270, 582, 1060
Eco47I GGWCC 1 cut(s) 678
Eco57I CTGAAG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 959
EcoNI CCTNNNNNAGG 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 3 cut(s) 270, 582, 1060
ErhI CCWWGG 3 cut(s) 270, 582, 1060
FaeI CATG 7 cut(s) 46, 421, 657, 662, 842, 893, 1015
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 438, 878
FatI CATG 7 cut(s) 42, 417, 653, 658, 838, 889, 1011
FauNDI CATATG 1 cut(s) 600
Fnu4HI GCNGC 1 cut(s) 1016
FokI GGATG 4 cut(s) 377, 522, 659, 824
Fsp4HI GCNGC 1 cut(s) 1016
FspBI CTAG 3 cut(s) 183, 246, 404
GluI GCNGC 1 cut(s) 1016
HaeIII GGCC 2 cut(s) 456, 932
HapII CCGG 1 cut(s) 380
HgaI GACGC 2 cut(s) 107, 227
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 7 cut(s) 46, 421, 657, 662, 842, 893, 1015
HindIII AAGCTT 2 cut(s) 546, 734
HinfI GANTC 7 cut(s) 4, 414, 481, 638, 650, 706, 968
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 858
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 5 cut(s) 82, 486, 505, 691, 1084
Hpy188III TCNNGA 7 cut(s) 22, 183, 380, 404, 659, 710, 740
Hpy8I GTNNAC 1 cut(s) 566
HpyCH4III ACNGT 1 cut(s) 988
HpyCH4V TGCA 9 cut(s) 233, 470, 724, 756, 772, 872, 928, 940, 1015
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 356, 778
HpyF3I CTNAG 4 cut(s) 174, 345, 477, 708
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 7 cut(s) 46, 421, 657, 662, 842, 893, 1015
Kpn2I TCCGGA 1 cut(s) 379
Kzo9I GATC 5 cut(s) 73, 82, 139, 242, 1079
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 1 cut(s) 1027
LweI GCATC 1 cut(s) 399
MaeI CTAG 3 cut(s) 183, 246, 404
MaeIII GTNAC 1 cut(s) 461
MalI GATC 5 cut(s) 75, 84, 141, 244, 1081
MboI GATC 5 cut(s) 73, 82, 139, 242, 1079
MboII GAAGA 5 cut(s) 236, 395, 928, 1082, 1100
MflI RGATCY 2 cut(s) 242, 1079
MluCI AATT 8 cut(s) 237, 260, 373, 516, 765, 885, 991, 1027
MlyI GAGTC 4 cut(s) 13, 423, 700, 977
MnlI CCTC 9 cut(s) 183, 220, 324, 991, 1001, 1042, 1045, 1060, 1066
MroI TCCGGA 1 cut(s) 379
MroXI GAANNNNTTC 2 cut(s) 668, 747
MseI TTAA 5 cut(s) 50, 206, 519, 732, 990
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 557
MvaI CCWGG 1 cut(s) 557
MwoI GCNNNNNNNGC 3 cut(s) 302, 356, 778
NdeI CATATG 1 cut(s) 600
NdeII GATC 5 cut(s) 73, 82, 139, 242, 1079
NlaIII CATG 7 cut(s) 46, 421, 657, 662, 842, 893, 1015
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 2 cut(s) 46, 1015
PaeI GCATGC 1 cut(s) 1015
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 877
PciI ACATGT 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 115
PdmI GAANNNNTTC 2 cut(s) 668, 747
PfeI GAWTC 3 cut(s) 481, 638, 650
PkrI GCNGC 1 cut(s) 1017
PleI GAGTC 4 cut(s) 12, 422, 700, 976
PpsI GAGTC 4 cut(s) 12, 422, 700, 976
PscI ACATGT 1 cut(s) 42
PshBI ATTAAT 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspPI GGNCC 1 cut(s) 678
PstI CTGCAG 2 cut(s) 472, 726
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 2 cut(s) 242, 1079
RsaI GTAC 1 cut(s) 795
RsaNI GTAC 1 cut(s) 794
SaqAI TTAA 5 cut(s) 50, 206, 519, 732, 990
SatI GCNGC 1 cut(s) 1016
Sau3AI GATC 5 cut(s) 73, 82, 139, 242, 1079
Sau96I GGNCC 1 cut(s) 678
SchI GAGTC 4 cut(s) 13, 423, 700, 977
ScrFI CCNGG 1 cut(s) 557
SfaNI GCATC 1 cut(s) 399
SfcI CTRYAG 2 cut(s) 468, 722
SinI GGWCC 1 cut(s) 678
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
SphI GCATGC 1 cut(s) 1015
Sse9I AATT 8 cut(s) 237, 260, 373, 516, 765, 885, 991, 1027
SsiI CCGC 2 cut(s) 394, 718
SspI AATATT 1 cut(s) 1094
SspMI CTAG 3 cut(s) 183, 246, 404
StyD4I CCNGG 1 cut(s) 555
StyI CCWWGG 3 cut(s) 270, 582, 1060
TaaI ACNGT 1 cut(s) 988
TasI AATT 8 cut(s) 237, 260, 373, 516, 765, 885, 991, 1027
TatI WGTACW 1 cut(s) 793
TfiI GAWTC 3 cut(s) 481, 638, 650
Tru1I TTAA 5 cut(s) 50, 206, 519, 732, 990
Tru9I TTAA 5 cut(s) 50, 206, 519, 732, 990
TscAI CASTG 2 cut(s) 361, 477
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 1 cut(s) 1015
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 5 cut(s) 537, 587, 663, 878, 1101
TspGWI ACGGA 1 cut(s) 1092
TspRI CASTG 2 cut(s) 361, 477
VpaK11BI GGWCC 1 cut(s) 678
VspI ATTAAT 1 cut(s) 519
XagI CCTNNNNNAGG 1 cut(s) 274
XapI RAATTY 2 cut(s) 765, 885
XbaI TCTAGA 2 cut(s) 182, 403
XceI RCATGY 2 cut(s) 46, 1015
XcmI CCANNNNNNNNNTGG 3 cut(s) 619, 991, 1026
XmnI GAANNNNTTC 2 cut(s) 668, 747
XspI CTAG 3 cut(s) 183, 246, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.