Rroxscaffold_4G00302990

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005803
Physical Location & Seq
Forward (+)
23359245 .. 23363974
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302990.1

Sequence Viewer

Length: 1125 bp
ATGTTGGAGTTAATTCATATGGCTCCATGTATGGATTGGTTGAACGGCCTTGAACCTGACATGGCGGTGCAGGTCCTAACTTGTTTGGACAATCCAAAGGATCTTGTTCGAATTAGTACCGTCTCACGCACTTGTCGTCAAATTGTGGTAGAAAATGGTCTGTGTAAGAAGCTTTGCTTGAGAATGTTTCCTCAGTTATCCAGAGTTGCTCATGTGATCGAGTCAAACAACCCTGATGCCAAAGAGTACGCAGAAGTTGGATCTAGCAATTCAAAGGAATGGGACACGTTGGAAATGGAGCATAGAGTCTATGCCTTCTTAGCTCATGCTTGTACATCTTTCCCTGCTAGGGATTGCATTTCAGTGGCAATCAGTGCTTCCAGCACTGACAATTATCCAGAGGAAAGCATTCAACATACTCTAGTGTCAAATGATATGGCGCGGAGAGCTTCATACTGGTCAAGTAAAGGCCAAAAGAACCCTGCAGTGCCTGAGATGCTGACATACAAGTTGAATTCTGATTGTGTTATAATTACTGAAATCAACATACATCCTTTCCAAGCTGACTTCCAGCATGGGTCACCCATATATTCTGCCAGTGGTGTTCGATTTCGTATGGGGCATGCCAAATTCCCTATTGATGCAGAGAGTGACCCCATGGAGGAATCATGCCATGATGACAAGTTTGAGTGGACTCACACTTCGCAAGTGTTCCCAATGGCTCAGGAAAACTGCTTGCAGACATTCAAGCTTCCAGAACCAGTTCTTTGCCTAGGTGGATTCTTGCAGATTGAGTTAGTGGGGAGGGTTCAGAAACAAGAAATAGATGACCTGTTCTATATATGTGTGTCTCACGTTCAAGTCAAGGGGAGGTCACTAGAACCTGCATTTAGTGTTGAAACCAATGTACCATCTGGTTTGTTTGTGTTGAAGAGACACACCAAGTTCAACCAACCAAGCTTACCTGAGAATGAGTCCAATGCTATCTCCACTGGTGATTTGGAGAGACATGTTATAGAATTGCTGCCCATTGTGAATGTGCTGCGTGCTACTGTAGATTTTGAGGAATATTATGAATGGGATGATGCCGATTATGAGACAGACGAGGAGGAGTTTGCGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

374

Amino Acids

42.4

Weight (kDa)

4.81

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 530
Acc36I ACCTGC 2 cut(s) 61, 892
AccII CGCG 1 cut(s) 442
AciI CCGC 3 cut(s) 65, 442, 1118
AclWI GGATC 2 cut(s) 108, 268
AcsI RAATTY 2 cut(s) 514, 629
AfaI GTAC 4 cut(s) 118, 248, 334, 909
AfiI CCNNNNNNNGG 2 cut(s) 349, 661
AflIII ACRYGT 2 cut(s) 285, 1009
AluBI AGCT 6 cut(s) 172, 323, 449, 563, 751, 960
AluI AGCT 6 cut(s) 172, 323, 449, 563, 751, 960
Alw26I GTCTC 5 cut(s) 127, 855, 928, 1000, 1091
AlwI GGATC 2 cut(s) 108, 268
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 2 cut(s) 46, 469
ApeKI GCWGC 2 cut(s) 1024, 1042
ApoI RAATTY 2 cut(s) 514, 629
ArsI GACNNNNNNTTYG 2 cut(s) 685, 717
Asp700I GAANNNNTTC 2 cut(s) 408, 762
AspA2I CCTAGG 1 cut(s) 772
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 573, 1007
AsuII TTCGAA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 772
BaeI ACNNNNGTAYC 2 cut(s) 891, 924
BbvI GCAGC 2 cut(s) 1011, 1029
BccI CCATC 1 cut(s) 919
BceAI ACGGC 1 cut(s) 61
BcoDI GTCTC 5 cut(s) 127, 855, 928, 1000, 1091
BfaI CTAG 5 cut(s) 264, 348, 422, 773, 878
BfmI CTRYAG 2 cut(s) 483, 1053
BfuAI ACCTGC 2 cut(s) 61, 892
BisI GCNGC 2 cut(s) 1025, 1043
BlnI CCTAGG 1 cut(s) 772
BlsI GCNGC 2 cut(s) 1026, 1044
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 24
BmsI GCATC 4 cut(s) 226, 486, 631, 1075
Bpu10I CCTNAGC 1 cut(s) 723
Bpu14I TTCGAA 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 199
BsaJI CCNNGG 2 cut(s) 657, 772
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 661
Bse1I ACTGG 4 cut(s) 461, 597, 761, 997
BseDI CCNNGG 2 cut(s) 657, 772
BseGI GGATG 2 cut(s) 550, 1087
BseLI CCNNNNNNNGG 2 cut(s) 349, 661
BseMII CTCAG 4 cut(s) 206, 483, 737, 957
BseNI ACTGG 4 cut(s) 461, 597, 761, 997
BseRI GAGGAG 2 cut(s) 1121, 1124
BseXI GCAGC 2 cut(s) 1011, 1029
BsgI GTGCAG 1 cut(s) 89
Bsh1236I CGCG 1 cut(s) 442
BshFI GGCC 2 cut(s) 48, 471
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 349, 661
BsmAI GTCTC 5 cut(s) 127, 855, 928, 1000, 1091
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 2 cut(s) 48, 471
Bsp119I TTCGAA 1 cut(s) 109
Bsp1407I TGTACA 1 cut(s) 332
Bsp143I GATC 3 cut(s) 100, 216, 260
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 3 cut(s) 65, 442, 1118
BspANI GGCC 2 cut(s) 48, 471
BspCNI CTCAG 4 cut(s) 205, 484, 736, 958
BspFNI CGCG 1 cut(s) 442
BspLI GGNNCC 1 cut(s) 24
BspMAI CTGCAG 1 cut(s) 487
BspMI ACCTGC 2 cut(s) 61, 892
BspPI GGATC 2 cut(s) 108, 268
BspT104I TTCGAA 1 cut(s) 109
BsrGI TGTACA 1 cut(s) 332
BsrI ACTGG 4 cut(s) 461, 597, 761, 997
BssECI CCNNGG 2 cut(s) 657, 772
BssMI GATC 3 cut(s) 100, 216, 260
BssT1I CCWWGG 2 cut(s) 657, 772
Bst4CI ACNGT 2 cut(s) 121, 1054
Bst6I CTCTTC 1 cut(s) 926
BstAPI GCANNNNNTGC 1 cut(s) 374
BstAUI TGTACA 1 cut(s) 332
BstBI TTCGAA 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 624, 737, 1047
BstDEI CTNAG 5 cut(s) 192, 319, 492, 723, 966
BstDSI CCRYGG 1 cut(s) 657
BstEII GGTNACC 1 cut(s) 579
BstF5I GGATG 2 cut(s) 550, 1087
BstFNI CGCG 1 cut(s) 442
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 3 cut(s) 103, 219, 263
BstMAI GTCTC 5 cut(s) 127, 855, 928, 1000, 1091
BstMBI GATC 3 cut(s) 100, 216, 260
BstMWI GCNNNNNNNGC 4 cut(s) 320, 374, 446, 496
BstNSI RCATGY 2 cut(s) 626, 1013
BstPI GGTNACC 1 cut(s) 579
BstSFI CTRYAG 2 cut(s) 483, 1053
BstUI CGCG 1 cut(s) 442
BstV1I GCAGC 2 cut(s) 1011, 1029
BstX2I RGATCY 2 cut(s) 100, 260
BstYI RGATCY 2 cut(s) 100, 260
BsuRI GGCC 2 cut(s) 48, 471
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 2 cut(s) 550, 1087
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 6 cut(s) 369, 379, 384, 492, 604, 990
BveI ACCTGC 2 cut(s) 61, 892
Cac8I GCNNGC 3 cut(s) 624, 737, 1047
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 73
Csp6I GTAC 4 cut(s) 117, 247, 333, 908
CviQI GTAC 4 cut(s) 117, 247, 333, 908
DdeI CTNAG 5 cut(s) 192, 319, 492, 723, 966
DpnI GATC 3 cut(s) 102, 218, 262
DpnII GATC 3 cut(s) 100, 216, 260
Eam1104I CTCTTC 1 cut(s) 926
EarI CTCTTC 1 cut(s) 926
Eco130I CCWWGG 2 cut(s) 657, 772
Eco47I GGWCC 1 cut(s) 73
Eco91I GGTNACC 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 73
EcoO65I GGTNACC 1 cut(s) 579
EcoRI GAATTC 1 cut(s) 514
EcoT14I CCWWGG 2 cut(s) 657, 772
ErhI CCWWGG 2 cut(s) 657, 772
Esp3I CGTCTC 1 cut(s) 127
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 296
FauNDI CATATG 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 1025, 1043
FokI GGATG 2 cut(s) 537, 1094
Fsp4HI GCNGC 2 cut(s) 1025, 1043
FspBI CTAG 5 cut(s) 264, 348, 422, 773, 878
GlaI GCGC 1 cut(s) 441
GluI GCNGC 2 cut(s) 1025, 1043
HaeIII GGCC 2 cut(s) 48, 471
HhaI GCGC 1 cut(s) 442
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HindIII AAGCTT 3 cut(s) 170, 749, 958
HinfI GANTC 6 cut(s) 221, 306, 665, 694, 780, 974
HphI GGTGA 2 cut(s) 573, 1007
Hpy166II GTNNAC 1 cut(s) 693
Hpy188I TCNGA 2 cut(s) 520, 813
Hpy188III TCNNGA 4 cut(s) 201, 398, 725, 755
Hpy8I GTNNAC 1 cut(s) 693
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 2 cut(s) 121, 1054
HpyCH4IV ACGT 2 cut(s) 287, 855
HpyCH4V TGCA 7 cut(s) 70, 357, 485, 644, 739, 787, 887
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 374, 446, 496
HpyF3I CTNAG 5 cut(s) 192, 319, 492, 723, 966
HpySE526I ACGT 2 cut(s) 287, 855
HspAI GCGC 1 cut(s) 440
Kzo9I GATC 3 cut(s) 100, 216, 260
LmnI GCTCC 2 cut(s) 28, 298
Lsp1109I GCAGC 2 cut(s) 1011, 1029
LweI GCATC 4 cut(s) 226, 486, 631, 1075
MaeI CTAG 5 cut(s) 264, 348, 422, 773, 878
MaeII ACGT 2 cut(s) 287, 855
MaeIII GTNAC 3 cut(s) 579, 650, 873
MalI GATC 3 cut(s) 102, 218, 262
MboI GATC 3 cut(s) 100, 216, 260
MboII GAAGA 1 cut(s) 943
MflI RGATCY 2 cut(s) 100, 260
MluCI AATT 9 cut(s) 12, 111, 141, 268, 391, 514, 531, 629, 1019
MlyI GAGTC 4 cut(s) 230, 315, 688, 983
MmeI TCCRAC 2 cut(s) 238, 270
MnlI CCTC 8 cut(s) 201, 394, 655, 798, 864, 1057, 1099, 1102
MroXI GAANNNNTTC 2 cut(s) 408, 762
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 2 cut(s) 65, 362
Mva1269I GAATGC 1 cut(s) 408
MvnI CGCG 1 cut(s) 442
MwoI GCNNNNNNNGC 4 cut(s) 320, 374, 446, 496
NcoI CCATGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 18
NdeII GATC 3 cut(s) 100, 216, 260
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 3 cut(s) 579, 650, 873
NspI RCATGY 2 cut(s) 626, 1013
NspV TTCGAA 1 cut(s) 109
PaeI GCATGC 1 cut(s) 626
PciI ACATGT 1 cut(s) 1009
PcsI WCGNNNNNNNCGW 1 cut(s) 133
PctI GAATGC 1 cut(s) 408
PdmI GAANNNNTTC 2 cut(s) 408, 762
PfeI GAWTC 2 cut(s) 665, 780
PkrI GCNGC 2 cut(s) 1026, 1044
PleI GAGTC 4 cut(s) 229, 314, 688, 982
PpsI GAGTC 4 cut(s) 229, 314, 688, 982
PpuMI RGGWCCY 1 cut(s) 73
PscI ACATGT 1 cut(s) 1009
PsiI TTATAA 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 73
PspEI GGTNACC 1 cut(s) 579
PspN4I GGNNCC 1 cut(s) 24
PspPI GGNCC 1 cut(s) 73
PspPPI RGGWCCY 1 cut(s) 73
PstI CTGCAG 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 2 cut(s) 100, 260
RsaI GTAC 4 cut(s) 118, 248, 334, 909
RsaNI GTAC 4 cut(s) 117, 247, 333, 908
RseI CAYNNNNRTG 2 cut(s) 65, 362
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 2 cut(s) 1025, 1043
Sau3AI GATC 3 cut(s) 100, 216, 260
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 4 cut(s) 230, 315, 688, 983
SfaNI GCATC 4 cut(s) 226, 486, 631, 1075
SfcI CTRYAG 2 cut(s) 483, 1053
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 2 cut(s) 65, 362
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
SphI GCATGC 1 cut(s) 626
Sse9I AATT 9 cut(s) 12, 111, 141, 268, 391, 514, 531, 629, 1019
SsiI CCGC 3 cut(s) 65, 442, 1118
SspI AATATT 1 cut(s) 1070
SspMI CTAG 5 cut(s) 264, 348, 422, 773, 878
StyI CCWWGG 2 cut(s) 657, 772
TaaI ACNGT 2 cut(s) 121, 1054
TaiI ACGT 2 cut(s) 290, 858
TaqI TCGA 3 cut(s) 109, 219, 607
TasI AATT 9 cut(s) 12, 111, 141, 268, 391, 514, 531, 629, 1019
TatI WGTACW 1 cut(s) 332
TfiI GAWTC 2 cut(s) 665, 780
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 6 cut(s) 369, 379, 391, 492, 604, 997
TseFI GTSAC 3 cut(s) 579, 650, 873
TseI GCWGC 2 cut(s) 1024, 1042
Tsp45I GTSAC 3 cut(s) 579, 650, 873
TspDTI ATGAA 3 cut(s) 5, 441, 1089
TspRI CASTG 6 cut(s) 369, 379, 391, 492, 604, 997
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 2 cut(s) 514, 629
XceI RCATGY 2 cut(s) 626, 1013
XcmI CCANNNNNNNNNTGG 2 cut(s) 33, 997
XmaJI CCTAGG 1 cut(s) 772
XmnI GAANNNNTTC 2 cut(s) 408, 762
XspI CTAG 5 cut(s) 264, 348, 422, 773, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.