pycom07g12350

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005803
Physical Location & Seq
Forward (+)
13668965 .. 13670693
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12350.1

Sequence Viewer

Length: 855 bp
ATGGGTCCTTGTATGGATTTCTTGAACTGGCTTGAACCTGACATGTCCTTAAATGTCCTAACTTGTTTGGACGATCTATATGATCTTATTCGTGTTAGTTCTGTCTCACGCTCTTGGCGTCATTTTGTGATAGAAAATGGTCTTTGTAAAAAGTTGTGCTTGAGAATGTTTCCTCAGTTATCTAGAGTTGCTGGTGTGGTTGAGTTAAACAGCCCTGTCGCAGAAGAGGTTGCAGAAGTTGGATCTAGCAAGTCAAAGGAATGGGAAACCTTGGAAAGGGAGCATAGGGTCTATGCTTTTTTAGCTTGTGGTTTTATTTCTTTCGATGTGAGGAGTTCTATTTCTAAGGCAATCAGTGCTTCCAGCACCGACAATTATCCAGAAGAAAGCATCCGCAATACTCTAGAACCAAGAGCCATGGTGGGACGGAGAGCTTCGTATTGGTCAAGTAAAGGCCAAAGTGACCCTGCAGTGCCTGAGATTCTGATATACAAGTTGGTGTCTGATTTAGTTGTAATTAATGAAATCAGCATACATCCTTTCCAAGCTTTTTTCCAGGCAGGTTTACCCATATATTCAGCCAAGGCTGTGCGATTTCGTATGGGTCGTGCCAAATCCCTTGTGGTTGTAGAGTGTGATTCAAAGGATGAATCATGTCATGAGAACAAGTTCGTATGGACCTACACTTCAGAAGTGTTCCCAATGACTCAGGAGAACCGCCTGCAGACATTTAAGCTTCCAGAACCAGTTCTTTGCATAGGTGGAATTTTGCAGATTGAGCTTCTGGATAGAGTACAGAGACAAGAAATGGATGGCTTGTTCTATATATGGTTAGTTCTGCGCTTATTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

285

Amino Acids

32.44

Weight (kDa)

5.55

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 14 - 55 9e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 551
AciI CCGC 2 cut(s) 394, 718
AclWI GGATC 1 cut(s) 250
AcsI RAATTY 1 cut(s) 765
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 1 cut(s) 795
AfiI CCNNNNNNNGG 1 cut(s) 276
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 3 cut(s) 25, 35, 642
AjnI CCWGG 1 cut(s) 555
AloI GAACNNNNNNTCC 2 cut(s) 803, 835
AluBI AGCT 5 cut(s) 305, 434, 548, 736, 781
AluI AGCT 5 cut(s) 305, 434, 548, 736, 781
Alw26I GTCTC 2 cut(s) 109, 793
AlwI GGATC 1 cut(s) 250
AlwNI CAGNNNCTG 1 cut(s) 476
AoxI GGCC 1 cut(s) 454
ApoI RAATTY 1 cut(s) 765
AseI ATTAAT 1 cut(s) 519
Asp700I GAANNNNTTC 2 cut(s) 668, 747
AspLEI GCGC 1 cut(s) 843
AspS9I GGNCC 2 cut(s) 5, 678
AvaII GGWCC 2 cut(s) 5, 678
BccI CCATC 1 cut(s) 806
BciT130I CCWGG 1 cut(s) 557
BcoDI GTCTC 2 cut(s) 109, 793
BfaI CTAG 3 cut(s) 183, 246, 404
BfmI CTRYAG 2 cut(s) 468, 722
BfuAI ACCTGC 1 cut(s) 551
Bme1390I CCNGG 1 cut(s) 557
Bme18I GGWCC 2 cut(s) 5, 678
BmgT120I GGNCC 2 cut(s) 5, 678
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 557
BmsI GCATC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 270, 417, 582
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse1I ACTGG 2 cut(s) 32, 746
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 3 cut(s) 270, 417, 582
BseGI GGATG 4 cut(s) 390, 535, 652, 817
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMII CTCAG 3 cut(s) 188, 468, 722
BseNI ACTGG 2 cut(s) 32, 746
BseRI GAGGAG 1 cut(s) 346
BshFI GGCC 1 cut(s) 456
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 2 cut(s) 109, 793
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 1 cut(s) 456
Bsp143I GATC 3 cut(s) 73, 82, 242
Bsp19I CCATGG 1 cut(s) 417
BspACI CCGC 2 cut(s) 394, 718
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 3 cut(s) 187, 469, 721
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 2 cut(s) 472, 726
BspMI ACCTGC 1 cut(s) 551
BspPI GGATC 1 cut(s) 250
BsrI ACTGG 2 cut(s) 32, 746
BssECI CCNNGG 3 cut(s) 270, 417, 582
BssMI GATC 3 cut(s) 73, 82, 242
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 3 cut(s) 270, 417, 582
Bst2UI CCWGG 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 4 cut(s) 174, 345, 477, 708
BstDSI CCRYGG 1 cut(s) 417
BstENI CCTNNNNNAGG 1 cut(s) 274
BstF5I GGATG 4 cut(s) 390, 535, 652, 817
BstHHI GCGC 1 cut(s) 843
BstKTI GATC 3 cut(s) 76, 85, 245
BstMAI GTCTC 2 cut(s) 109, 793
BstMBI GATC 3 cut(s) 73, 82, 242
BstMWI GCNNNNNNNGC 3 cut(s) 302, 356, 778
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 555
BstSFI CTRYAG 2 cut(s) 468, 722
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 1 cut(s) 456
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 4 cut(s) 390, 535, 652, 817
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 2 cut(s) 361, 477
BveI ACCTGC 1 cut(s) 551
Cac8I GCNNGC 1 cut(s) 722
CaiI CAGNNNCTG 1 cut(s) 476
CciI TCATGA 1 cut(s) 658
CfoI GCGC 1 cut(s) 843
Cfr13I GGNCC 2 cut(s) 5, 678
CseI GACGC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 794
CviAII CATG 4 cut(s) 43, 418, 654, 659
CviQI GTAC 1 cut(s) 794
DdeI CTNAG 4 cut(s) 174, 345, 477, 708
DpnI GATC 3 cut(s) 75, 84, 244
DpnII GATC 3 cut(s) 73, 82, 242
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco130I CCWWGG 3 cut(s) 270, 417, 582
Eco47I GGWCC 2 cut(s) 5, 678
Eco57I CTGAAG 1 cut(s) 672
EcoNI CCTNNNNNAGG 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 3 cut(s) 270, 417, 582
ErhI CCWWGG 3 cut(s) 270, 417, 582
FaeI CATG 4 cut(s) 46, 421, 657, 662
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 438
FatI CATG 4 cut(s) 42, 417, 653, 658
FokI GGATG 4 cut(s) 377, 522, 659, 824
FspBI CTAG 3 cut(s) 183, 246, 404
GlaI GCGC 1 cut(s) 842
HaeIII GGCC 1 cut(s) 456
HgaI GACGC 1 cut(s) 107
HhaI GCGC 1 cut(s) 843
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 4 cut(s) 46, 421, 657, 662
Hin6I GCGC 1 cut(s) 841
HinP1I GCGC 1 cut(s) 841
HindIII AAGCTT 2 cut(s) 546, 734
HinfI GANTC 4 cut(s) 481, 638, 650, 706
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 3 cut(s) 486, 505, 691
Hpy188III TCNNGA 9 cut(s) 22, 183, 380, 404, 659, 710, 740, 785, 852
Hpy8I GTNNAC 1 cut(s) 566
HpyCH4V TGCA 5 cut(s) 233, 470, 724, 756, 772
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 356, 778
HpyF3I CTNAG 4 cut(s) 174, 345, 477, 708
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 4 cut(s) 46, 421, 657, 662
HspAI GCGC 1 cut(s) 841
Kzo9I GATC 3 cut(s) 73, 82, 242
LmnI GCTCC 1 cut(s) 280
LweI GCATC 1 cut(s) 399
MaeI CTAG 3 cut(s) 183, 246, 404
MaeIII GTNAC 1 cut(s) 461
MalI GATC 3 cut(s) 75, 84, 244
MboI GATC 3 cut(s) 73, 82, 242
MboII GAAGA 2 cut(s) 236, 395
MflI RGATCY 1 cut(s) 242
MluCI AATT 3 cut(s) 373, 516, 765
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 3 cut(s) 183, 220, 324
MroXI GAANNNNTTC 2 cut(s) 668, 747
MseI TTAA 4 cut(s) 50, 206, 519, 732
MspR9I CCNGG 1 cut(s) 557
MvaI CCWGG 1 cut(s) 557
MwoI GCNNNNNNNGC 3 cut(s) 302, 356, 778
NcoI CCATGG 1 cut(s) 417
NdeII GATC 3 cut(s) 73, 82, 242
NlaIII CATG 4 cut(s) 46, 421, 657, 662
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 1 cut(s) 46
PagI TCATGA 1 cut(s) 658
PciI ACATGT 1 cut(s) 42
PcsI WCGNNNNNNNCGW 2 cut(s) 115, 604
PdmI GAANNNNTTC 2 cut(s) 668, 747
PfeI GAWTC 3 cut(s) 481, 638, 650
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
PpuMI RGGWCCY 1 cut(s) 5
PscI ACATGT 1 cut(s) 42
PshBI ATTAAT 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 5, 678
PspPPI RGGWCCY 1 cut(s) 5
PstI CTGCAG 2 cut(s) 472, 726
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 1 cut(s) 242
RsaI GTAC 1 cut(s) 795
RsaNI GTAC 1 cut(s) 794
SaqAI TTAA 4 cut(s) 50, 206, 519, 732
Sau3AI GATC 3 cut(s) 73, 82, 242
Sau96I GGNCC 2 cut(s) 5, 678
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 1 cut(s) 557
SfaNI GCATC 1 cut(s) 399
SfcI CTRYAG 2 cut(s) 468, 722
SinI GGWCC 2 cut(s) 5, 678
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 3 cut(s) 373, 516, 765
SsiI CCGC 2 cut(s) 394, 718
SspMI CTAG 3 cut(s) 183, 246, 404
StyD4I CCNGG 1 cut(s) 555
StyI CCWWGG 3 cut(s) 270, 417, 582
TaqI TCGA 1 cut(s) 324
TasI AATT 3 cut(s) 373, 516, 765
TatI WGTACW 1 cut(s) 793
TfiI GAWTC 3 cut(s) 481, 638, 650
Tru1I TTAA 4 cut(s) 50, 206, 519, 732
Tru9I TTAA 4 cut(s) 50, 206, 519, 732
TscAI CASTG 2 cut(s) 361, 477
TseFI GTSAC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 2 cut(s) 537, 663
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 2 cut(s) 361, 477
VpaK11BI GGWCC 2 cut(s) 5, 678
VspI ATTAAT 1 cut(s) 519
XagI CCTNNNNNAGG 1 cut(s) 274
XapI RAATTY 1 cut(s) 765
XbaI TCTAGA 2 cut(s) 182, 403
XceI RCATGY 1 cut(s) 46
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XmnI GAANNNNTTC 2 cut(s) 668, 747
XspI CTAG 3 cut(s) 183, 246, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.