MD01G1102500.v1.1

Snf7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003851
Physical Location & Seq
Forward (+)
21481840 .. 21484484
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1102500.v1.1.491

Sequence Viewer

Length: 690 bp
ATGAACAAAGTCAAGAGCTTGTTGAAGCCGAAACCTAACCCGTCGGAGCAACTCAGAGAATGGCAGCGGCGCCTCCGCCATGAGTGCCGCAACATGGAGCGCCAAATCAGAGATATACAGAAAGAAGAGAAGGCTGTACATAAAACTATCAGAGAGGCTGCCAAGAGAAATGACATGGCCACAGCCAAGGCACTTGCAAAGGAAATTGTGATGTCGAGGAAAGTTGTGAATCGGCTTCATGAGAATAAGGCACAACTGAATTCGATATCTATGCACCTTGGAGAAAGTGTTGCACTTGCCCGTACCGTGGGTCATCTGTCAAAAAGTACAGAGGTTATGAAACTTGTCAATAACCTCATGAAAGCTCCAGAAATGGCCGTCACGATGCATGAATTTACCAAAGAAATCACCAAGGCTGGCGTGATTGAGGAATTCGTAGATGATGCTCTTGATACGGCATTAGATTCAGAAGATATAGAAGAGGAAACCGAAGAAGAAGTTGAAAGGGTCCTCAGTGAAATAGCTGGTGAGACGGCTGCACAGCTCCCAGAAGCTGTGCGGAAGGAGAGGACAAAGGTAGCAGCCCAGAGAGCCAGTGCGTCACAACAAGAAGAAGCTATAGCAGAGGGTGCAGACAATGAGGAAGAGTTGGAAGCACTGAGGGCGCGGCTTGCCCAAGTGAGATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04217 map04144 map04217
Pfam Domains
Protein Families
Snf7

Protein Analysis

230

Amino Acids

25.91

Weight (kDa)

5.83

Isoelectric Point (pI)

60.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snf7 PF03357 20 - 188 1e-31 Snf7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 69
AccII CGCG 1 cut(s) 667
AciI CCGC 5 cut(s) 67, 76, 88, 559, 667
AcoI YGGCCR 2 cut(s) 177, 375
AcsI RAATTY 3 cut(s) 259, 392, 431
AcyI GRCGYC 1 cut(s) 70
AfaI GTAC 3 cut(s) 138, 304, 328
AfiI CCNNNNNNNGG 2 cut(s) 94, 307
AgsI TTSAA 2 cut(s) 25, 503
AluBI AGCT 6 cut(s) 18, 365, 524, 544, 554, 617
AluI AGCT 6 cut(s) 18, 365, 524, 544, 554, 617
Alw26I GTCTC 1 cut(s) 524
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 2 cut(s) 177, 375
ApeKI GCWGC 4 cut(s) 64, 158, 536, 581
ApoI RAATTY 3 cut(s) 259, 392, 431
AspLEI GCGC 3 cut(s) 72, 102, 667
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 2 cut(s) 400, 539
AvaII GGWCC 1 cut(s) 508
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 69
BbvI GCAGC 4 cut(s) 76, 145, 523, 593
BceAI ACGGC 3 cut(s) 362, 471, 549
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BcoDI GTCTC 1 cut(s) 524
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 2 cut(s) 73, 103
BisI GCNGC 7 cut(s) 65, 68, 88, 159, 537, 582, 668
BlsI GCNGC 7 cut(s) 66, 69, 89, 160, 538, 583, 669
Bme18I GGWCC 1 cut(s) 508
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 2 cut(s) 71, 509
BmsI GCATC 2 cut(s) 375, 433
BpmI CTGGAG 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 70
BsaJI CCNNGG 4 cut(s) 186, 277, 306, 411
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 307
Bse1I ACTGG 1 cut(s) 594
BseDI CCNNGG 4 cut(s) 186, 277, 306, 411
BseLI CCNNNNNNNGG 2 cut(s) 94, 307
BseMII CTCAG 3 cut(s) 67, 526, 650
BseNI ACTGG 1 cut(s) 594
BseXI GCAGC 4 cut(s) 76, 145, 523, 593
BsgI GTGCAG 2 cut(s) 522, 651
Bsh1236I CGCG 1 cut(s) 667
BshFI GGCC 2 cut(s) 179, 377
BshNI GGYRCC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 94, 307
BsmAI GTCTC 1 cut(s) 524
BsmBI CGTCTC 1 cut(s) 524
BsnI GGCC 2 cut(s) 179, 377
Bsp1407I TGTACA 1 cut(s) 136
Bsp143I GATC 1 cut(s) 683
BspACI CCGC 5 cut(s) 67, 76, 88, 559, 667
BspANI GGCC 2 cut(s) 179, 377
BspCNI CTCAG 3 cut(s) 66, 525, 651
BspFNI CGCG 1 cut(s) 667
BspHI TCATGA 2 cut(s) 238, 357
BspLI GGNNCC 2 cut(s) 71, 509
BspT107I GGYRCC 1 cut(s) 69
BsrGI TGTACA 1 cut(s) 136
BsrI ACTGG 1 cut(s) 594
BssECI CCNNGG 4 cut(s) 186, 277, 306, 411
BssMI GATC 1 cut(s) 683
BssNI GRCGYC 1 cut(s) 70
BssT1I CCWWGG 3 cut(s) 186, 277, 411
Bst4CI ACNGT 1 cut(s) 307
Bst6I CTCTTC 3 cut(s) 120, 474, 639
BstACI GRCGYC 1 cut(s) 70
BstAPI GCANNNNNTGC 1 cut(s) 629
BstAUI TGTACA 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 418, 672
BstDEI CTNAG 3 cut(s) 53, 512, 659
BstDSI CCRYGG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 667
BstH2I RGCGCY 2 cut(s) 73, 103
BstHHI GCGC 3 cut(s) 72, 102, 667
BstKTI GATC 1 cut(s) 686
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 1 cut(s) 683
BstMWI GCNNNNNNNGC 5 cut(s) 84, 590, 629, 662, 671
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 1 cut(s) 667
BstV1I GCAGC 4 cut(s) 76, 145, 523, 593
BsuRI GGCC 2 cut(s) 179, 377
BtgI CCRYGG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 520, 601, 656
Cac8I GCNNGC 2 cut(s) 418, 672
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 2 cut(s) 238, 357
CfoI GCGC 3 cut(s) 72, 102, 667
Cfr13I GGNCC 1 cut(s) 508
CseI GACGC 1 cut(s) 588
Csp6I GTAC 3 cut(s) 137, 303, 327
CviAII CATG 6 cut(s) 80, 94, 175, 239, 358, 389
CviQI GTAC 3 cut(s) 137, 303, 327
DdeI CTNAG 3 cut(s) 53, 512, 659
DinI GGCGCC 1 cut(s) 71
DpnI GATC 1 cut(s) 685
DpnII GATC 1 cut(s) 683
EaeI YGGCCR 2 cut(s) 177, 375
Eam1104I CTCTTC 3 cut(s) 120, 474, 639
EarI CTCTTC 3 cut(s) 120, 474, 639
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 3 cut(s) 186, 277, 411
Eco32I GATATC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 508
EcoO109I RGGNCCY 1 cut(s) 508
EcoRI GAATTC 2 cut(s) 259, 431
EcoRV GATATC 1 cut(s) 267
EcoT14I CCWWGG 3 cut(s) 186, 277, 411
EcoT22I ATGCAT 1 cut(s) 390
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
ErhI CCWWGG 3 cut(s) 186, 277, 411
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 6 cut(s) 83, 97, 178, 242, 361, 392
FatI CATG 6 cut(s) 79, 93, 174, 238, 357, 388
Fnu4HI GCNGC 7 cut(s) 65, 68, 88, 159, 537, 582, 668
Fsp4HI GCNGC 7 cut(s) 65, 68, 88, 159, 537, 582, 668
GlaI GCGC 3 cut(s) 71, 101, 666
GluI GCNGC 7 cut(s) 65, 68, 88, 159, 537, 582, 668
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 2 cut(s) 73, 103
HaeIII GGCC 2 cut(s) 179, 377
HgaI GACGC 1 cut(s) 588
HhaI GCGC 3 cut(s) 72, 102, 667
Hin1I GRCGYC 1 cut(s) 70
Hin1II CATG 6 cut(s) 83, 97, 178, 242, 361, 392
Hin6I GCGC 3 cut(s) 70, 100, 665
HinP1I GCGC 3 cut(s) 70, 100, 665
HinfI GANTC 2 cut(s) 229, 464
HphI GGTGA 2 cut(s) 400, 539
Hpy188I TCNGA 5 cut(s) 46, 56, 110, 152, 469
Hpy188III TCNNGA 6 cut(s) 13, 239, 358, 368, 382, 449
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 124, 556
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4V TGCA 6 cut(s) 197, 274, 293, 388, 539, 632
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 590, 629, 662, 671
HpyF3I CTNAG 3 cut(s) 53, 512, 659
Hsp92I GRCGYC 1 cut(s) 70
Hsp92II CATG 6 cut(s) 83, 97, 178, 242, 361, 392
HspAI GCGC 3 cut(s) 70, 100, 665
KasI GGCGCC 1 cut(s) 69
Kzo9I GATC 1 cut(s) 683
LmnI GCTCC 4 cut(s) 46, 97, 370, 549
LpnPI CCDG 6 cut(s) 381, 402, 510, 561, 599, 607
Lsp1109I GCAGC 4 cut(s) 76, 145, 523, 593
LweI GCATC 2 cut(s) 375, 433
MaeIII GTNAC 2 cut(s) 379, 600
MalI GATC 1 cut(s) 685
MboI GATC 1 cut(s) 683
MboII GAAGA 7 cut(s) 137, 482, 491, 503, 506, 623, 656
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 4 cut(s) 204, 259, 392, 431
MluNI TGGCCA 1 cut(s) 179
Mly113I GGCGCC 1 cut(s) 70
MmeI TCCRAC 2 cut(s) 24, 630
Mox20I TGGCCA 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 390
MscI TGGCCA 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 67
MvnI CGCG 1 cut(s) 667
MwoI GCNNNNNNNGC 5 cut(s) 84, 590, 629, 662, 671
NarI GGCGCC 1 cut(s) 70
NdeII GATC 1 cut(s) 683
NlaIII CATG 6 cut(s) 83, 97, 178, 242, 361, 392
NlaIV GGNNCC 2 cut(s) 71, 509
NmuCI GTSAC 2 cut(s) 379, 600
NsiI ATGCAT 1 cut(s) 390
PagI TCATGA 2 cut(s) 238, 357
PfeI GAWTC 2 cut(s) 229, 464
PkrI GCNGC 7 cut(s) 66, 69, 89, 160, 538, 583, 669
PluTI GGCGCC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
PspN4I GGNNCC 2 cut(s) 71, 509
PspPI GGNCC 1 cut(s) 508
PspPPI RGGWCCY 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 554
RsaI GTAC 3 cut(s) 138, 304, 328
RsaNI GTAC 3 cut(s) 137, 303, 327
SatI GCNGC 7 cut(s) 65, 68, 88, 159, 537, 582, 668
Sau3AI GATC 1 cut(s) 683
Sau96I GGNCC 1 cut(s) 508
SfaNI GCATC 2 cut(s) 375, 433
SfcI CTRYAG 1 cut(s) 618
SfoI GGCGCC 1 cut(s) 71
SinI GGWCC 1 cut(s) 508
Sse9I AATT 4 cut(s) 204, 259, 392, 431
SsiI CCGC 5 cut(s) 67, 76, 88, 559, 667
SspDI GGCGCC 1 cut(s) 69
StyI CCWWGG 3 cut(s) 186, 277, 411
TaaI ACNGT 1 cut(s) 307
TaqI TCGA 2 cut(s) 215, 263
TasI AATT 4 cut(s) 204, 259, 392, 431
TatI WGTACW 2 cut(s) 136, 326
TauI GCSGC 3 cut(s) 70, 90, 670
TfiI GAWTC 2 cut(s) 229, 464
TscAI CASTG 3 cut(s) 520, 601, 663
TseFI GTSAC 2 cut(s) 379, 600
TseI GCWGC 4 cut(s) 64, 158, 536, 581
Tsp45I GTSAC 2 cut(s) 379, 600
TspDTI ATGAA 5 cut(s) 17, 227, 353, 374, 405
TspRI CASTG 3 cut(s) 520, 601, 663
VpaK11BI GGWCC 1 cut(s) 508
XapI RAATTY 3 cut(s) 259, 392, 431
Zsp2I ATGCAT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.