MD01G1172400.v1.1

bromo domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005918
Physical Location & Seq
Reverse (-)
27444448 .. 27451004
6557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1172400.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGCCTTTATCTTTCTTCAACTTTAACTACAGGAAAGACTTGTACAATATGTTTGCAAAACCAGTAAACCCACAAGAGGTTAAAGGGTACAATGATATCATTAAAGAGCCAATGGATTTCGGCACGATTTCAGAAAAACTCAGTGGAGGAAGCTACAGAACACTGGAAGAATTTCAGCACGATGTGTTTTTGGTATTCAGTAATGCAATGTCATACAACGCTCCATCTTCCATCTTTAATCGAAAGGCTTGTGATATGAAAAATCTAGCGGTGGAATTGTTTGAAGCCCTCGAGAAAGATTATAAAAGTTTTGAAAGTGTATGTTCCTCGAAAATATCAAGAGCTGGTAAGAGGAGCAAGAACATCATGAACAGTTATGCTGATAGGTCAACTTATGGGATGCCTTTAAGGGGTCGGTCGCAAGGGGTTGCCGAGCAGAAGTTACAAACAGCCGGAGTTCTTAATCCTTGTAATTCAACTTCAAACTTTCAGTTTAGTGATCTAGATCTTAATGTGGCATTATTTCAGAATGGCATCATAAATACTCAACCTTCAAATATCCTTCGGAGTTCCACCCCACCTCTATACTCCGGTGCTCAAACAAACATTGAGCACCATAATGGTCAGTATATTGGGACTGTGCCCTCAATCAATGCCTTGTTTAACAACAGCATTGGGACTGATGTTGGTAGGGGTAGGAATCCTTTTATAGCTGGTCTGCCTGCTTCAGATCTAAGGGGGAACTGTGAAAAGGTTTATGGCCAGTCACCGCCTTCTTCGATAAGGTGGCATTGCCCTCCAATTATTTCTAATTCAAGTTGGAGCCCAGCCTTTGGCTCCACACATGGGCTGAACTCAAATTGTTTTGGAGGTGGACAGCATCAATTTCAACCTATGGGTGGTAGTGGAAGCACACTGTATGGAAGTAATAGTTTCGAGCAGGCAAGCCAAGGTCTGAACGGGTTGAGGCAAGCGGAGATAGGACCACAAATACCTGACTTCAAACCATTCCAGCAGCAAGGGCAAGGCTCGGGCACACCAGCCTCTGGAATCAGCATGAGGCCTATAAATTTGTCAGACATAGATCAATGGTTTGGGCATGGTCGGAGTGGAGGGGGGTCAAACACTGGAGCTCCTCCTGCAAGCTCACTAGGGCTAGGTGCCGCAGAAACAAACATGTTTCCTTGTGACTTTCGGCACTCTGGGGGATCAAACGCTGGAGCTCTTCCTGCAAGCTCTGAGGGATGTGATTGGAACCCGTGGAACTTTCGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain

Protein Analysis

426

Amino Acids

45.97

Weight (kDa)

7.59

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 9 - 79 5.7e-20 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 1160
AccB7I CCANNNNNTGG 2 cut(s) 833, 1046
AciI CCGC 4 cut(s) 269, 770, 974, 1164
AclWI GGATC 1 cut(s) 1216
AcoI YGGCCR 2 cut(s) 760, 1271
AcsI RAATTY 2 cut(s) 170, 1069
AcuI CTGAAG 1 cut(s) 711
AdeI CACNNNGTG 1 cut(s) 184
AfaI GTAC 2 cut(s) 44, 89
AfiI CCNNNNNNNGG 6 cut(s) 76, 410, 833, 846, 899, 1046
AflIII ACRYGT 1 cut(s) 1176
AgsI TTSAA 9 cut(s) 19, 284, 314, 477, 483, 555, 816, 890, 1003
AluBI AGCT 7 cut(s) 153, 344, 713, 1133, 1146, 1223, 1236
AluI AGCT 7 cut(s) 153, 344, 713, 1133, 1146, 1223, 1236
Alw21I GWGCWC 4 cut(s) 598, 615, 1135, 1225
AlwI GGATC 1 cut(s) 1216
AlwNI CAGNNNCTG 1 cut(s) 1046
Ama87I CYCGRG 2 cut(s) 290, 1030
AoxI GGCC 3 cut(s) 760, 1061, 1271
ApeKI GCWGC 1 cut(s) 1015
ApoI RAATTY 2 cut(s) 170, 1069
Asp700I GAANNNNTTC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 983
AsuHPI GGTGA 1 cut(s) 759
AvaI CYCGRG 2 cut(s) 290, 1030
AvaII GGWCC 1 cut(s) 983
BaeGI GKGCMC 2 cut(s) 645, 1037
BalI TGGCCA 1 cut(s) 762
BanI GGYRCC 1 cut(s) 1160
BanII GRGCYC 3 cut(s) 827, 1135, 1225
BarI GAAGNNNNNNTAC 2 cut(s) 463, 495
Bbv12I GWGCWC 4 cut(s) 598, 615, 1135, 1225
BbvI GCAGC 1 cut(s) 1027
BccI CCATC 2 cut(s) 232, 239
BfaI CTAG 4 cut(s) 266, 503, 1151, 1157
BfmI CTRYAG 2 cut(s) 28, 154
BglII AGATCT 2 cut(s) 505, 730
BisI GCNGC 2 cut(s) 1016, 1164
BlsI GCNGC 2 cut(s) 1017, 1165
Bme18I GGWCC 1 cut(s) 983
BmeT110I CYCGRG 2 cut(s) 290, 1030
BmgT120I GGNCC 1 cut(s) 983
BmiI GGNNCC 4 cut(s) 824, 838, 1162, 1256
BmsI GCATC 3 cut(s) 390, 543, 889
BpmI CTGGAG 2 cut(s) 1149, 1239
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 949, 1259
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 2 cut(s) 1117, 1147
Bsc4I CCNNNNNNNGG 6 cut(s) 76, 410, 833, 846, 899, 1046
Bse1I ACTGG 4 cut(s) 62, 168, 763, 1132
Bse3DI GCAATG 2 cut(s) 213, 790
Bse8I GATNNNNATC 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 949, 1259
BseGI GGATG 2 cut(s) 405, 1250
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 6 cut(s) 76, 410, 833, 846, 899, 1046
BseMI GCAATG 2 cut(s) 213, 790
BseMII CTCAG 2 cut(s) 154, 1230
BseNI ACTGG 4 cut(s) 62, 168, 763, 1132
BseRI GAGGAG 2 cut(s) 367, 1125
BseSI GKGCMC 2 cut(s) 645, 1037
BseXI GCAGC 1 cut(s) 1027
BseYI CCCAGC 1 cut(s) 826
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 3 cut(s) 762, 1063, 1273
BshNI GGYRCC 1 cut(s) 1160
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 4 cut(s) 598, 615, 1135, 1225
BsiHKCI CYCGRG 2 cut(s) 290, 1030
BsiSI CCGG 2 cut(s) 453, 591
BslFI GGGAC 2 cut(s) 649, 691
BslI CCNNNNNNNGG 6 cut(s) 76, 410, 833, 846, 899, 1046
BsmFI GGGAC 2 cut(s) 649, 691
BsnI GGCC 3 cut(s) 762, 1063, 1273
BsoBI CYCGRG 2 cut(s) 290, 1030
Bsp1286I GDGCHC 7 cut(s) 598, 615, 645, 827, 1037, 1135, 1225
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 5 cut(s) 499, 505, 730, 1084, 1208
BspACI CCGC 4 cut(s) 269, 770, 974, 1164
BspANI GGCC 3 cut(s) 762, 1063, 1273
BspCNI CTCAG 2 cut(s) 153, 1231
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 4 cut(s) 824, 838, 1162, 1256
BspPI GGATC 1 cut(s) 1216
BspQI GCTCTTC 1 cut(s) 1230
BspT107I GGYRCC 1 cut(s) 1160
BsrDI GCAATG 2 cut(s) 213, 790
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 4 cut(s) 62, 168, 763, 1132
BssECI CCNNGG 2 cut(s) 949, 1259
BssMI GATC 5 cut(s) 499, 505, 730, 1084, 1208
BssT1I CCWWGG 1 cut(s) 949
Bst4CI ACNGT 4 cut(s) 374, 640, 746, 918
Bst6I CTCTTC 1 cut(s) 1230
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 6 cut(s) 723, 942, 946, 972, 1144, 1234
BstDEI CTNAG 3 cut(s) 140, 734, 1239
BstDSI CCRYGG 1 cut(s) 1259
BstF5I GGATG 2 cut(s) 405, 1250
BstKTI GATC 5 cut(s) 502, 508, 733, 1087, 1211
BstMBI GATC 5 cut(s) 499, 505, 730, 1084, 1208
BstMCI CGRYCG 1 cut(s) 419
BstMWI GCNNNNNNNGC 3 cut(s) 1021, 1139, 1229
BstNSI RCATGY 1 cut(s) 1180
BstSFI CTRYAG 2 cut(s) 28, 154
BstSLI GKGCMC 2 cut(s) 645, 1037
BstV1I GCAGC 1 cut(s) 1027
BstX2I RGATCY 2 cut(s) 505, 730
BstYI RGATCY 2 cut(s) 505, 730
BsuRI GGCC 3 cut(s) 762, 1063, 1273
BtgI CCRYGG 1 cut(s) 1259
BtsCI GGATG 2 cut(s) 405, 1250
BtsIMutI CAGTG 4 cut(s) 148, 161, 914, 1125
Cac8I GCNNGC 6 cut(s) 723, 942, 946, 972, 1144, 1234
CaiI CAGNNNCTG 1 cut(s) 1046
CciI TCATGA 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 983
Csp6I GTAC 2 cut(s) 43, 88
CviAII CATG 6 cut(s) 367, 845, 1057, 1100, 1177, 1275
CviQI GTAC 2 cut(s) 43, 88
DdeI CTNAG 3 cut(s) 140, 734, 1239
DpnI GATC 5 cut(s) 501, 507, 732, 1086, 1210
DpnII GATC 5 cut(s) 499, 505, 730, 1084, 1208
DraIII CACNNNGTG 1 cut(s) 184
EaeI YGGCCR 2 cut(s) 760, 1271
Eam1104I CTCTTC 1 cut(s) 1230
EarI CTCTTC 1 cut(s) 1230
Ecl136II GAGCTC 2 cut(s) 1133, 1223
Eco130I CCWWGG 1 cut(s) 949
Eco147I AGGCCT 1 cut(s) 1063
Eco24I GRGCYC 3 cut(s) 827, 1135, 1225
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 983
Eco53kI GAGCTC 2 cut(s) 1133, 1223
Eco57I CTGAAG 1 cut(s) 711
Eco88I CYCGRG 2 cut(s) 290, 1030
EcoICRI GAGCTC 2 cut(s) 1133, 1223
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 949
EcoT38I GRGCYC 3 cut(s) 827, 1135, 1225
ErhI CCWWGG 1 cut(s) 949
FaeI CATG 6 cut(s) 370, 848, 1060, 1103, 1180, 1278
FaqI GGGAC 2 cut(s) 649, 691
FatI CATG 6 cut(s) 366, 844, 1056, 1099, 1176, 1274
Fnu4HI GCNGC 2 cut(s) 1016, 1164
FokI GGATG 2 cut(s) 412, 1257
FriOI GRGCYC 3 cut(s) 827, 1135, 1225
Fsp4HI GCNGC 2 cut(s) 1016, 1164
FspBI CTAG 4 cut(s) 266, 503, 1151, 1157
GluI GCNGC 2 cut(s) 1016, 1164
GsaI CCCAGC 1 cut(s) 830
GsuI CTGGAG 2 cut(s) 1149, 1239
HaeIII GGCC 3 cut(s) 762, 1063, 1273
HapII CCGG 2 cut(s) 453, 591
Hin1II CATG 6 cut(s) 370, 848, 1060, 1103, 1180, 1278
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HinfI GANTC 2 cut(s) 700, 1050
HpaII CCGG 2 cut(s) 453, 591
HphI GGTGA 1 cut(s) 759
Hpy166II GTNNAC 3 cut(s) 67, 390, 875
Hpy188I TCNGA 8 cut(s) 133, 528, 567, 730, 957, 1078, 1107, 1240
Hpy188III TCNNGA 5 cut(s) 292, 339, 367, 503, 1047
Hpy8I GTNNAC 3 cut(s) 67, 390, 875
HpyAV CCTTC 3 cut(s) 561, 572, 783
HpyCH4III ACNGT 4 cut(s) 374, 640, 746, 918
HpyCH4V TGCA 4 cut(s) 56, 206, 1142, 1232
HpyF10VI GCNNNNNNNGC 3 cut(s) 1021, 1139, 1229
HpyF3I CTNAG 3 cut(s) 140, 734, 1239
Hsp92II CATG 6 cut(s) 370, 848, 1060, 1103, 1180, 1278
Kzo9I GATC 5 cut(s) 499, 505, 730, 1084, 1208
LguI GCTCTTC 1 cut(s) 1230
LmnI GCTCC 7 cut(s) 226, 354, 822, 842, 1130, 1138, 1220
Lsp1109I GCAGC 1 cut(s) 1027
LweI GCATC 3 cut(s) 390, 543, 889
MaeI CTAG 4 cut(s) 266, 503, 1151, 1157
MaeIII GTNAC 3 cut(s) 441, 765, 1187
MalI GATC 5 cut(s) 501, 507, 732, 1086, 1210
MboI GATC 5 cut(s) 499, 505, 730, 1084, 1208
MboII GAAGA 5 cut(s) 7, 179, 219, 768, 1217
MflI RGATCY 2 cut(s) 505, 730
MhlI GDGCHC 7 cut(s) 598, 615, 645, 827, 1037, 1135, 1225
MlsI TGGCCA 1 cut(s) 762
MluCI AATT 8 cut(s) 170, 275, 472, 801, 811, 859, 884, 1069
MluNI TGGCCA 1 cut(s) 762
MmeI TCCRAC 2 cut(s) 800, 1085
Mox20I TGGCCA 1 cut(s) 762
MroXI GAANNNNTTC 1 cut(s) 171
MscI TGGCCA 1 cut(s) 762
MseI TTAA 8 cut(s) 24, 81, 102, 237, 407, 462, 510, 663
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 762
MspI CCGG 2 cut(s) 453, 591
MwoI GCNNNNNNNGC 3 cut(s) 1021, 1139, 1229
NdeII GATC 5 cut(s) 499, 505, 730, 1084, 1208
NlaIII CATG 6 cut(s) 370, 848, 1060, 1103, 1180, 1278
NlaIV GGNNCC 4 cut(s) 824, 838, 1162, 1256
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 2 cut(s) 765, 1187
NspI RCATGY 1 cut(s) 1180
PaeR7I CTCGAG 1 cut(s) 290
PagI TCATGA 1 cut(s) 366
PceI AGGCCT 1 cut(s) 1063
PciI ACATGT 1 cut(s) 1176
PciSI GCTCTTC 1 cut(s) 1230
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 2 cut(s) 700, 1050
PflMI CCANNNNNTGG 2 cut(s) 833, 1046
PkrI GCNGC 2 cut(s) 1017, 1165
PscI ACATGT 1 cut(s) 1176
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 2 cut(s) 1135, 1225
PspFI CCCAGC 1 cut(s) 826
PspN4I GGNNCC 4 cut(s) 824, 838, 1162, 1256
PspPI GGNCC 1 cut(s) 983
PstNI CAGNNNCTG 1 cut(s) 1046
PsuI RGATCY 2 cut(s) 505, 730
RsaI GTAC 2 cut(s) 44, 89
RsaNI GTAC 2 cut(s) 43, 88
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 2 cut(s) 1135, 1225
SapI GCTCTTC 1 cut(s) 1230
SaqAI TTAA 8 cut(s) 24, 81, 102, 237, 407, 462, 510, 663
SatI GCNGC 2 cut(s) 1016, 1164
Sau3AI GATC 5 cut(s) 499, 505, 730, 1084, 1208
Sau96I GGNCC 1 cut(s) 983
SduI GDGCHC 7 cut(s) 598, 615, 645, 827, 1037, 1135, 1225
SfaNI GCATC 3 cut(s) 390, 543, 889
SfcI CTRYAG 2 cut(s) 28, 154
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 1 cut(s) 983
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 8 cut(s) 170, 275, 472, 801, 811, 859, 884, 1069
SseBI AGGCCT 1 cut(s) 1063
SsiI CCGC 4 cut(s) 269, 770, 974, 1164
SspMI CTAG 4 cut(s) 266, 503, 1151, 1157
SstI GAGCTC 2 cut(s) 1135, 1225
StuI AGGCCT 1 cut(s) 1063
StyI CCWWGG 1 cut(s) 949
TaaI ACNGT 4 cut(s) 374, 640, 746, 918
TaqI TCGA 5 cut(s) 241, 291, 329, 779, 936
TaqII GACCGA 1 cut(s) 405
TasI AATT 8 cut(s) 170, 275, 472, 801, 811, 859, 884, 1069
TatI WGTACW 1 cut(s) 42
TauI GCSGC 1 cut(s) 1166
TfiI GAWTC 2 cut(s) 700, 1050
Tru1I TTAA 8 cut(s) 24, 81, 102, 237, 407, 462, 510, 663
Tru9I TTAA 8 cut(s) 24, 81, 102, 237, 407, 462, 510, 663
TscAI CASTG 4 cut(s) 148, 168, 921, 1132
TseFI GTSAC 2 cut(s) 765, 1187
TseI GCWGC 1 cut(s) 1015
Tsp45I GTSAC 2 cut(s) 765, 1187
TspDTI ATGAA 2 cut(s) 272, 383
TspRI CASTG 4 cut(s) 148, 168, 921, 1132
Van91I CCANNNNNTGG 2 cut(s) 833, 1046
VpaK11BI GGWCC 1 cut(s) 983
XapI RAATTY 2 cut(s) 170, 1069
XbaI TCTAGA 1 cut(s) 502
XceI RCATGY 1 cut(s) 1180
XhoI CTCGAG 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 4 cut(s) 266, 503, 1151, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.