RchiOBHm_Chr1g0372791

platelet degranulation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005918
Physical Location & Seq
Reverse (-)
61319761 .. 61325103
5343 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59676

Sequence Viewer

Length: 519 bp
ATGATGTGGAATGGGGACCGTAGACAGAGTTTACGTATATTTGCACTTGAAACACAGAAGAAGCAGCAAAAAGAGAACCAGAATCTGAATCAGGGAAAAAAGGCTGGTAGTACTAGTACCAAAGATCATCAGACAAATGCTGCTGTTATGTCATCTGGAAATCCTTCACCAGAAAGAAGCAAGCTTCAACTGTTGCTTGGAGAGATAACATACCTCGGAGCCAGTAAATGCAGAAGAGCAAAACTCAATGGAGGAAGCTACAAAGCACCGCAAGAATTTGAGCATGATGCAACACTGGTATGGAGCAATGCTATGCTTTTCAACGATTCAAGTACCCTTTATTATAAAGAGGCTCGTTTGATAAGAAATCTAGCGACGAAGTTGTTTAATTCTTTGAAAACAGACCCTGAGAGCTTTGTGGAAATATCAAAATCTGGATGCAGAGGTGGAAGAAGATCCAAATATTCTGAAGTAGATCAACGCTATACCTACCGGCCTCTGAATAAATTGCTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain

Protein Analysis

172

Amino Acids

19.57

Weight (kDa)

9.9

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 79 - 119 1.6e-07 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AccI GTMKAC 1 cut(s) 22
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 450
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 489
AfaI GTAC 3 cut(s) 112, 118, 334
AgsI TTSAA 5 cut(s) 50, 188, 322, 330, 397
AhlI ACTAGT 1 cut(s) 113
AluBI AGCT 3 cut(s) 184, 258, 414
AluI AGCT 3 cut(s) 184, 258, 414
AlwI GGATC 1 cut(s) 450
AlwNI CAGNNNCTG 2 cut(s) 85, 407
AoxI GGCC 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 64, 140
ApoI RAATTY 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 159
AvaII GGWCC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 76, 127
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 2 cut(s) 114, 371
BisI GCNGC 2 cut(s) 65, 141
BlsI GCNGC 2 cut(s) 66, 142
BmcAI AGTACT 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 2 cut(s) 17, 220
BmsI GCATC 2 cut(s) 277, 428
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BsaAI YACGTR 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 214
Bse118I RCCGGY 1 cut(s) 492
Bse1I ACTGG 2 cut(s) 222, 300
Bse3DI GCAATG 1 cut(s) 313
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 443
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 2 cut(s) 222, 300
BseXI GCAGC 2 cut(s) 76, 127
BshFI GGCC 1 cut(s) 496
BsiSI CCGG 1 cut(s) 493
BslFI GGGAC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 1 cut(s) 496
Bsp143I GATC 3 cut(s) 124, 455, 475
BspACI CCGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 496
BspCNI CTCAG 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 17, 220
BspPI GGATC 1 cut(s) 450
BspQI GCTCTTC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 492
BsrI ACTGG 2 cut(s) 222, 300
BssAI RCCGGY 1 cut(s) 492
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 3 cut(s) 124, 455, 475
Bst4CI ACNGT 2 cut(s) 20, 192
Bst6I CTCTTC 1 cut(s) 229
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 443
BstKTI GATC 3 cut(s) 127, 458, 478
BstMBI GATC 3 cut(s) 124, 455, 475
BstSNI TACGTA 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 76, 127
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BsuRI GGCC 1 cut(s) 496
BtsCI GGATG 1 cut(s) 443
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 182
CaiI CAGNNNCTG 2 cut(s) 85, 407
Cfr10I RCCGGY 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 111, 117, 333
CviAII CATG 1 cut(s) 284
CviJI RGCY 7 cut(s) 104, 184, 221, 258, 353, 414, 496
CviKI_1 RGCY 7 cut(s) 104, 184, 221, 258, 353, 414, 496
CviQI GTAC 3 cut(s) 111, 117, 333
DdeI CTNAG 1 cut(s) 408
DpnI GATC 3 cut(s) 126, 457, 477
DpnII GATC 3 cut(s) 124, 455, 475
Eam1104I CTCTTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 229
Eco105I TACGTA 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 16
Eco57I CTGAAG 1 cut(s) 489
FaeI CATG 1 cut(s) 287
FaiI YATR 8 cut(s) 38, 149, 211, 285, 301, 314, 345, 486
FaqI GGGAC 1 cut(s) 29
FatI CATG 1 cut(s) 283
FblI GTMKAC 1 cut(s) 22
Fnu4HI GCNGC 2 cut(s) 65, 141
FokI GGATG 1 cut(s) 450
Fsp4HI GCNGC 2 cut(s) 65, 141
FspBI CTAG 2 cut(s) 114, 371
GluI GCNGC 2 cut(s) 65, 141
HaeIII GGCC 1 cut(s) 496
HapII CCGG 1 cut(s) 493
Hin1II CATG 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 3 cut(s) 82, 88, 326
HpaII CCGG 1 cut(s) 493
HphI GGTGA 1 cut(s) 159
Hpy166II GTNNAC 2 cut(s) 23, 32
Hpy188I TCNGA 5 cut(s) 87, 132, 218, 469, 501
Hpy188III TCNNGA 2 cut(s) 156, 435
Hpy8I GTNNAC 2 cut(s) 23, 32
Hpy99I CGWCG 1 cut(s) 379
HpyAV CCTTC 1 cut(s) 174
HpyCH4III ACNGT 2 cut(s) 20, 192
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 4 cut(s) 44, 231, 290, 441
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 1 cut(s) 287
Kzo9I GATC 3 cut(s) 124, 455, 475
LguI GCTCTTC 1 cut(s) 229
LmnI GCTCC 2 cut(s) 218, 303
Lsp1109I GCAGC 2 cut(s) 76, 127
LweI GCATC 2 cut(s) 277, 428
MaeI CTAG 2 cut(s) 114, 371
MaeII ACGT 1 cut(s) 34
MalI GATC 3 cut(s) 126, 457, 477
MboI GATC 3 cut(s) 124, 455, 475
MboII GAAGA 4 cut(s) 70, 246, 462, 465
MflI RGATCY 1 cut(s) 455
MluCI AATT 4 cut(s) 275, 388, 506, 514
MnlI CCTC 5 cut(s) 224, 245, 343, 437, 507
MroXI GAANNNNTTC 1 cut(s) 163
MseI TTAA 2 cut(s) 387, 517
MslI CAYNNNNRTG 1 cut(s) 298
MspI CCGG 1 cut(s) 493
NdeII GATC 3 cut(s) 124, 455, 475
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 2 cut(s) 17, 220
PciSI GCTCTTC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 163
PfeI GAWTC 3 cut(s) 82, 88, 326
PkrI GCNGC 2 cut(s) 66, 142
Ppu21I YACGTR 1 cut(s) 35
PsiI TTATAA 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 17, 220
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 2 cut(s) 85, 407
PsuI RGATCY 1 cut(s) 455
RsaI GTAC 3 cut(s) 112, 118, 334
RsaNI GTAC 3 cut(s) 111, 117, 333
RseI CAYNNNNRTG 1 cut(s) 298
SapI GCTCTTC 1 cut(s) 229
SaqAI TTAA 2 cut(s) 387, 517
SatI GCNGC 2 cut(s) 65, 141
Sau3AI GATC 3 cut(s) 124, 455, 475
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 112
SetI ASST 7 cut(s) 37, 186, 216, 260, 416, 448, 491
SfaNI GCATC 2 cut(s) 277, 428
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 298
SnaBI TACGTA 1 cut(s) 35
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 4 cut(s) 275, 388, 506, 514
SsiI CCGC 1 cut(s) 269
SspI AATATT 1 cut(s) 464
SspMI CTAG 2 cut(s) 114, 371
TaaI ACNGT 2 cut(s) 20, 192
TaiI ACGT 1 cut(s) 37
TasI AATT 4 cut(s) 275, 388, 506, 514
TatI WGTACW 1 cut(s) 110
TfiI GAWTC 3 cut(s) 82, 88, 326
Tru1I TTAA 2 cut(s) 387, 517
Tru9I TTAA 2 cut(s) 387, 517
TscAI CASTG 1 cut(s) 300
TseI GCWGC 2 cut(s) 64, 140
TspRI CASTG 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 163
XspI CTAG 2 cut(s) 114, 371
ZrmI AGTACT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.