Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005164

Physical Location & Seq

Genomic Coordinates Forward (+)

28027280 .. 28031929

Gene Structure 4650 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1181200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGGAGGCTGCTCTCCAATCAAACCCTAACCTTCCTCCTCCAATCGAATCCCTAGATCCTCACCCTCCTCATTCCGATTTCAATCCTCCTCCCCCTGCAGAAACCCTAGCCTCAGATTCTAATGGCCCGTCTCAACCGCCGCCCACCGAAACCCTAGCTTCCTATGCTCAAGACTCCTCCACGAATCCCAAGACCGCTGCTGAGAACGGTAATTCGACTAACGCCTGCAAGGCGCTTGCTATTATGAAGCCGCTGTTGTCGGAAAACGGATTGACCAATACCACTAGCGGCAACGAAAAGGATGGCTCGGGCGGGGAGGAGGATACGACTAGCAGGCGCCGCCGCCGCAGCCGGTGGGATCCTCAGCCCGAGTCGGACAATCAGAGTGGTGGTGGCGAGACAGGCAGTGGCACGAGGAAGAGGAAGTCGCGGTGGGCGGATGAGGAGCCGAAGCAGGCGATTCAGCTGCCGGATTTCATGGGAGGTATTGAGTTTGATCCAGAAATTCAAGCTTTGAATAGTAGGTTGCTTGAGATTAGTCGAATGCTGTCATCGGGGTTGCCCTTAGATGATCGTCCCGAAGGGGCCAGGTCTCCTTCGCCGGAACCGATATATGATAACATGGGAATTAGGATTAATACTAGAGAGTTCCGTGCCCGTGAGAGGTTGAACAAAGAGAGACAGGAAATTATTGCCCAGATTATTAAGAGGAACCCAGCATTTAAGCCCCCGGCTGATTACAGGCCACCCAAGCTTCACAAGAAGCTTTACATACCGATGAAGGAATATCCCGGATACAATTTTATCGGACTTATCATTGGGCCGAGGGGTAATACCCAGAAGAGGATGGAGAAAGAAACTGGTGCAAAAATTGTCATTAGGGGTAAAGGTTCGGTGAAAGAGGGTAGGTCACAACAGAAGAGGGATTTGAAACCTGACCCTTCCGAGAATGAGGACTTGCATGTTTTGGTTGAGGCCGATACACAAGACGCTCTTGATGCCGCGGCTGGAATGGTGGAGAAACTCTTGCAGCCTGTCGATGAGGTGTTGAATGAGCATAAGAGGCAACAACTTAGGGAACTTGCGTCATTGAACGGGACAATAAGGGATGAGGAGTATTGTAGGTTGTGTGGGGAGCCGGGGCACCGGCAGTATGCTTGTCCATCACGTACTTCAACCTTCAAGAGCGATGTTCTTTGTAAGATATGTGGTGATGGTGGGCATCCAACCATTGATTGCCCAGTGAAGGGTACAACTGGGAAGAAAATGGATGATGAGTATCAGAACTTCTTAGCTGAGTTAGGGGGGACTGTTCCTGAATCTGCAACTAAGCAGACCGCTACATTGGCACTTGGCCCAGGCAACAGTACATCAAGCAATCCTCCTTGGGCTAACAGTACTGGCAGTGCTAGTGCATCATCACATCCAGGCTTGGGATCAATTGGAGTCAAGCCAGTCAAGGACTATGACGAGACAAACTTGTACATTGGATACTTGCCGCCTACTTTTGATGATGATGGTTTGATCCGATTGTTTTCACCATTTGGTGATATTGTGATGGCCAAAGTTATCAAGGACCGAGTTACTGGAGCGAGTAAAGGTTATGGTTTTGTGAAGTACGGAGATGTTCAAATGGCTAATAATGCAATTGCAAGCATGAATGGTTATCGCCTAGACCAGCGAACCATTGCTGTCAGAGTTGCTGGCAAGCCTCCTCAGCCTGCTGCGCCTCCTGGACCTCCAGCATCGGCACTGCCCACATACCCGGTTCCCCAGCCTGTTGGTGCCTATCCATCCCAGCAGTTTACCGCAGGTGGCCCTCTTGGGAATGCTCCACCTACTAGCTACGTGGGTACTCCTGTTCCTTGGGGACCCCCTGTTCCTCCTCCTTATGCTCCTTATGCTCCACCTCCTCCACATCCCCCTGGTTCAACCATGTATCCTCCTCCCATGCCGGGTCAACATATGGCTCCTTATGGTGCACGATACCCTCCTCCACCTGGGGCACCACCTCAACCGGTGACTTCAAGTGAAGCACAACAGAATTATCCCCCTCCAGGAGTTCAATCCGAACACAATATCTCTAACCAATCCCTACAATCTAATATGTATGGTGGCTCCTCAGCACCAATGCCACCAAACGGCCAACACACTTATCCTGCTGCTTCGTATGGTTACTCATCTTACTACAATGCAGCTCCACCACCTCCGCCTCCCCCCGCCCATGTACCTGGATCAACTGCGGACCAATCTCAGAACATTGGTAACGTTCCATGGGCCACGAGTGCTCCGGTTCCCCCTCATGCTTCTTCTGCGGAAAGGACAACTTACGGTGCAGATGCAGAGTATGAGAAGTTCATGTCAGAAATGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010467 GO:0016070 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0071704 GO:0090304 GO:0097305 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1 RRM_1 SF1-HH

Protein Analysis

792

Amino Acids

85.02

Weight (kDa)

6.04

Isoelectric Point (pI)

59.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SF1-HH	PF16275	141 - 248	1.6e-25	Splicing factor 1 helix-hairpin domain
KH-I_KHDC4-BBP	PF22675	262 - 344	2.7e-24	KHDC4/BBP-like, KH-domain type I
RRM_1	PF00076	494 - 563	3.1e-18	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51300 AT5G51300 AT5G51300
fragaria_vesca	FvH4_6g31120 FvH4_6g31120 FvH4_7g27230 FvH4_7g27230
malus_domestica	MD01G1181200.v1.1 MD07G1251300.v1.1
prunus_persica	Prupe.2G275100_v2.0.a1
pyrus_communis	pycom01g19120 pycom07g22400
rosa_chinensis	RchiOBHm_Chr1g0374011 RchiOBHm_Chr2g0137831
rosa_laevigata	RLG00000019679 RLG00000026786
rosa_roxburghii	Rroxscaffold_2G00106780 Rroxscaffold_4G00283830
rosa_rugosa	Rorug01G0380300 Rorug02G0346500
rosa_samantha	Rh1AG390700 Rh1BG354800 Rh1CG367900 Rh1DG385500 Rh2AG393200 Rh2BG400800 Rh2CG379900 Rh2DG415800
rosa_wichuraiana	Rw1G034450 Rw2G032210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1802
Acc36I	ACCTGC	1 cut(s)	1802
AccB1I	GGYRCC	4 cut(s)	336, 1143, 1784, 2005
AccII	CGCG	2 cut(s)	430, 1004
AclI	AACGTT	1 cut(s)	2268
AclWI	GGATC	7 cut(s)	50, 353, 366, 491, 1444, 1519, 2242
AcoI	YGGCCR	2 cut(s)	1560, 2143
AcsI	RAATTY	1 cut(s)	504
AcyI	GRCGYC	1 cut(s)	337
AfaI	GTAC	8 cut(s)	1171, 1252, 1369, 1399, 1484, 1619, 1855, 2229
AfiI	CCNNNNNNNGG	9 cut(s)	663, 843, 1246, 1247, 1433, 1955, 2000, 2057, 2141
AgeI	ACCGGT	1 cut(s)	2017
AjnI	CCWGG	8 cut(s)	587, 1357, 1426, 1732, 1924, 1999, 2056, 2230
AloI	GAACNNNNNNTCC	2 cut(s)	1863, 1895
AluBI	AGCT	8 cut(s)	158, 466, 512, 754, 766, 1295, 1845, 2198
AluI	AGCT	8 cut(s)	158, 466, 512, 754, 766, 1295, 1845, 2198
Alw21I	GWGCWC	2 cut(s)	1984, 2290
Alw26I	GTCTC	5 cut(s)	135, 392, 597, 673, 1466
Alw44I	GTGCAC	1 cut(s)	1980
AlwI	GGATC	7 cut(s)	50, 353, 366, 491, 1444, 1519, 2242
Ama87I	CYCGRG	2 cut(s)	307, 368
ApaLI	GTGCAC	1 cut(s)	1980
ApeKI	GCWGC	8 cut(s)	8, 197, 348, 466, 1030, 1724, 2162, 2195
ApoI	RAATTY	1 cut(s)	504
AseI	ATTAAT	1 cut(s)	636
AsiGI	ACCGGT	1 cut(s)	2017
AspLEI	GCGC	3 cut(s)	235, 339, 1729
AsuC2I	CCSGG	5 cut(s)	731, 792, 1140, 1766, 1956
AsuHPI	GGTGA	6 cut(s)	53, 907, 1223, 1530, 1559, 2032
AvaI	CYCGRG	2 cut(s)	307, 368
AvaII	GGWCC	4 cut(s)	1576, 1736, 1871, 2245
BaeGI	GKGCMC	4 cut(s)	658, 1146, 1984, 2008
BaeI	ACNNNNGTAYC	2 cut(s)	1845, 1878
BalI	TGGCCA	1 cut(s)	1562
BamHI	GGATCC	1 cut(s)	358
BanI	GGYRCC	4 cut(s)	336, 1143, 1784, 2005
BarI	GAAGNNNNNNTAC	2 cut(s)	1271, 1303
BauI	CACGAG	2 cut(s)	412, 2281
Bbv12I	GWGCWC	2 cut(s)	1984, 2290
BbvCI	CCTCAGC	3 cut(s)	363, 1716, 2122
BbvI	GCAGC	7 cut(s)	184, 360, 453, 1042, 1711, 2149, 2207
BccI	CCATC	7 cut(s)	296, 841, 1171, 1208, 1511, 1552, 1801
BceAI	ACGGC	1 cut(s)	2158
BcgI	CGANNNNNNTGC	2 cut(s)	448, 482
BciT130I	CCWGG	8 cut(s)	589, 1359, 1428, 1734, 1926, 2001, 2058, 2232
BciVI	GTATCC	4 cut(s)	316, 788, 1484, 1950
BcnI	CCSGG	5 cut(s)	731, 792, 1140, 1766, 1956
BcoDI	GTCTC	5 cut(s)	135, 392, 597, 673, 1466
BfaI	CTAG	9 cut(s)	53, 107, 155, 285, 330, 642, 1410, 1673, 1842
BfmI	CTRYAG	1 cut(s)	96
BfoI	RGCGCY	2 cut(s)	236, 340
BfuAI	ACCTGC	1 cut(s)	1802
BfuI	GTATCC	4 cut(s)	316, 788, 1484, 1950
BglI	GCCNNNNNGGC	1 cut(s)	230
BmcAI	AGTACT	1 cut(s)	1399
Bme18I	GGWCC	4 cut(s)	1576, 1736, 1871, 2245
BmeT110I	CYCGRG	2 cut(s)	307, 368
BmrI	ACTGGG	2 cut(s)	1235, 1266
BmsI	GCATC	5 cut(s)	988, 1231, 1424, 1754, 2329
BmuI	ACTGGG	2 cut(s)	1235, 1266
BpmI	CTGGAG	3 cut(s)	1608, 1725, 2040
Bpu10I	CCTNAGC	3 cut(s)	363, 1716, 2122
BpuEI	CTTGAG	2 cut(s)	153, 551
BpuMI	CCSGG	5 cut(s)	731, 792, 1140, 1766, 1956
BsaAI	YACGTR	2 cut(s)	1169, 1849
BsaBI	GATNNNNATC	2 cut(s)	81, 1278
BsaHI	GRCGYC	1 cut(s)	337
BsaI	GGTCTC	1 cut(s)	597
BsaWI	WCCGGW	2 cut(s)	2017, 2290
BsaXI	ACNNNNNCTCC	2 cut(s)	2272, 2302
Bsc4I	CCNNNNNNNGG	9 cut(s)	663, 843, 1246, 1247, 1433, 1955, 2000, 2057, 2141
Bse118I	RCCGGY	3 cut(s)	351, 1146, 2017
Bse1I	ACTGG	6 cut(s)	865, 1241, 1261, 1405, 1454, 1591
Bse3DI	GCAATG	1 cut(s)	1686
Bse8I	GATNNNNATC	2 cut(s)	81, 1278
BseBI	CCWGG	8 cut(s)	589, 1359, 1428, 1734, 1926, 2001, 2058, 2232
BseGI	GGATG	9 cut(s)	307, 445, 852, 1114, 1222, 1276, 1423, 1793, 1918
BseJI	GATNNNNATC	2 cut(s)	81, 1278
BseLI	CCNNNNNNNGG	9 cut(s)	663, 843, 1246, 1247, 1433, 1955, 2000, 2057, 2141
BseMI	GCAATG	1 cut(s)	1686
BseMII	CTCAG	7 cut(s)	126, 192, 377, 1287, 1730, 2136, 2267
BseNI	ACTGG	6 cut(s)	865, 1241, 1261, 1405, 1454, 1591
BseSI	GKGCMC	4 cut(s)	658, 1146, 1984, 2008
BseXI	GCAGC	7 cut(s)	184, 360, 453, 1042, 1711, 2149, 2207
BseYI	CCCAGC	3 cut(s)	715, 1773, 1797
BsgI	GTGCAG	1 cut(s)	2355
Bsh1236I	CGCG	2 cut(s)	430, 1004
BshNI	GGYRCC	4 cut(s)	336, 1143, 1784, 2005
BshTI	ACCGGT	1 cut(s)	2017
BsiHKAI	GWGCWC	2 cut(s)	1984, 2290
BsiHKCI	CYCGRG	2 cut(s)	307, 368
BslFI	GGGAC	4 cut(s)	561, 1111, 1321, 1884
BslI	CCNNNNNNNGG	9 cut(s)	663, 843, 1246, 1247, 1433, 1955, 2000, 2057, 2141
BsmAI	GTCTC	5 cut(s)	135, 392, 597, 673, 1466
BsmBI	CGTCTC	1 cut(s)	135
BsmFI	GGGAC	4 cut(s)	561, 1111, 1321, 1884
BsmI	GAATGC	2 cut(s)	549, 1834
Bso31I	GGTCTC	1 cut(s)	597
BsoBI	CYCGRG	2 cut(s)	307, 368
Bsp1286I	GDGCHC	5 cut(s)	658, 1146, 1984, 2008, 2290
Bsp1407I	TGTACA	1 cut(s)	1482
Bsp143I	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
Bsp19I	CCATGG	1 cut(s)	2273
BspCNI	CTCAG	7 cut(s)	125, 193, 376, 1288, 1729, 2135, 2266
BspFNI	CGCG	2 cut(s)	430, 1004
BspMAI	CTGCAG	1 cut(s)	100
BspMI	ACCTGC	1 cut(s)	1802
BspPI	GGATC	7 cut(s)	50, 353, 366, 491, 1444, 1519, 2242
BspT107I	GGYRCC	4 cut(s)	336, 1143, 1784, 2005
BspTNI	GGTCTC	1 cut(s)	597
BsrDI	GCAATG	1 cut(s)	1686
BsrFI	RCCGGY	3 cut(s)	351, 1146, 2017
BsrGI	TGTACA	1 cut(s)	1482
BsrI	ACTGG	6 cut(s)	865, 1241, 1261, 1405, 1454, 1591
BssAI	RCCGGY	3 cut(s)	351, 1146, 2017
BssMI	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
BssNI	GRCGYC	1 cut(s)	337
BssSI	CACGAG	2 cut(s)	412, 2281
BssT1I	CCWWGG	3 cut(s)	1385, 1865, 2273
Bst2BI	CACGAG	2 cut(s)	412, 2281
Bst2UI	CCWGG	8 cut(s)	589, 1359, 1428, 1734, 1926, 2001, 2058, 2232
Bst4CI	ACNGT	5 cut(s)	209, 1312, 1367, 1397, 2333
Bst6I	CTCTTC	3 cut(s)	413, 836, 914
BstACI	GRCGYC	1 cut(s)	337
BstAUI	TGTACA	1 cut(s)	1482
BstBAI	YACGTR	2 cut(s)	1169, 1849
BstC8I	GCNNGC	8 cut(s)	226, 237, 335, 456, 1654, 1705, 1709, 1722
BstDSI	CCRYGG	2 cut(s)	1002, 2273
BstF5I	GGATG	9 cut(s)	307, 445, 852, 1114, 1222, 1276, 1423, 1793, 1918
BstFNI	CGCG	2 cut(s)	430, 1004
BstH2I	RGCGCY	2 cut(s)	236, 340
BstHHI	GCGC	3 cut(s)	235, 339, 1729
BstKTI	GATC	7 cut(s)	58, 361, 499, 574, 1439, 1527, 2237
BstMAI	GTCTC	5 cut(s)	135, 392, 597, 673, 1466
BstMBI	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
BstNI	CCWGG	8 cut(s)	589, 1359, 1428, 1734, 1926, 2001, 2058, 2232
BstNSI	RCATGY	1 cut(s)	965
BstSFI	CTRYAG	1 cut(s)	96
BstSLI	GKGCMC	4 cut(s)	658, 1146, 1984, 2008
BstUI	CGCG	2 cut(s)	430, 1004
BstV1I	GCAGC	7 cut(s)	184, 360, 453, 1042, 1711, 2149, 2207
BstX2I	RGATCY	2 cut(s)	55, 358
BstXI	CCANNNNNNTGG	2 cut(s)	1781, 2231
BstYI	RGATCY	2 cut(s)	55, 358
BsuI	GTATCC	4 cut(s)	316, 788, 1484, 1950
BtgI	CCRYGG	2 cut(s)	1002, 2273
BtgZI	GCGATG	1 cut(s)	1203
BtsCI	GGATG	9 cut(s)	307, 445, 852, 1114, 1222, 1276, 1423, 1793, 1918
BtsI	GCAGTG	3 cut(s)	412, 1411, 1751
BtsIMutI	CAGTG	4 cut(s)	412, 1248, 1411, 1751
BveI	ACCTGC	1 cut(s)	1802
Cac8I	GCNNGC	8 cut(s)	226, 237, 335, 456, 1654, 1705, 1709, 1722
CfoI	GCGC	3 cut(s)	235, 339, 1729
Cfr10I	RCCGGY	3 cut(s)	351, 1146, 2017
Cfr42I	CCGCGG	1 cut(s)	1005
CseI	GACGC	2 cut(s)	998, 1074
Csp6I	GTAC	8 cut(s)	1170, 1251, 1368, 1398, 1483, 1618, 1854, 2228
CspAI	ACCGGT	1 cut(s)	2017
CspCI	CAANNNNNGTGG	2 cut(s)	367, 402
CviQI	GTAC	8 cut(s)	1170, 1251, 1368, 1398, 1483, 1618, 1854, 2228
DinI	GGCGCC	1 cut(s)	338
DpnI	GATC	7 cut(s)	57, 360, 498, 573, 1438, 1526, 2236
DpnII	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
EaeI	YGGCCR	2 cut(s)	1560, 2143
Eam1104I	CTCTTC	3 cut(s)	413, 836, 914
EarI	CTCTTC	3 cut(s)	413, 836, 914
EciI	GGCGGA	2 cut(s)	452, 2199
Eco130I	CCWWGG	3 cut(s)	1385, 1865, 2273
Eco31I	GGTCTC	1 cut(s)	597
Eco47I	GGWCC	4 cut(s)	1576, 1736, 1871, 2245
Eco88I	CYCGRG	2 cut(s)	307, 368
EcoO109I	RGGNCCY	1 cut(s)	1871
EcoRII	CCWGG	8 cut(s)	587, 1357, 1426, 1732, 1924, 1999, 2056, 2230
EcoT14I	CCWWGG	3 cut(s)	1385, 1865, 2273
EgeI	GGCGCC	1 cut(s)	338
EheI	GGCGCC	1 cut(s)	338
ErhI	CCWWGG	3 cut(s)	1385, 1865, 2273
Esp3I	CGTCTC	1 cut(s)	135
FalI	AAGNNNNNCTT	4 cut(s)	978, 1010, 2311, 2343
FaqI	GGGAC	4 cut(s)	561, 1111, 1321, 1884
FauI	CCCGC	2 cut(s)	305, 2227
FauNDI	CATATG	1 cut(s)	1965
FokI	GGATG	9 cut(s)	314, 452, 859, 1121, 1209, 1283, 1410, 1780, 1905
FspBI	CTAG	9 cut(s)	53, 107, 155, 285, 330, 642, 1410, 1673, 1842
GlaI	GCGC	3 cut(s)	234, 338, 1728
GsaI	CCCAGC	3 cut(s)	719, 1777, 1801
GsuI	CTGGAG	3 cut(s)	1608, 1725, 2040
HaeII	RGCGCY	2 cut(s)	236, 340
HgaI	GACGC	2 cut(s)	998, 1074
HhaI	GCGC	3 cut(s)	235, 339, 1729
Hin1I	GRCGYC	1 cut(s)	337
Hin6I	GCGC	3 cut(s)	233, 337, 1727
HinP1I	GCGC	3 cut(s)	233, 337, 1727
HincII	GTYRAC	1 cut(s)	1961
HindII	GTYRAC	1 cut(s)	1961
HindIII	AAGCTT	3 cut(s)	510, 752, 764
HinfI	GANTC	8 cut(s)	47, 116, 173, 184, 371, 460, 1319, 1446
HphI	GGTGA	6 cut(s)	53, 907, 1223, 1530, 1559, 2032
Hpy166II	GTNNAC	3 cut(s)	1806, 1961, 1982
Hpy188III	TCNNGA	6 cut(s)	170, 500, 578, 995, 1183, 1316
Hpy8I	GTNNAC	3 cut(s)	1806, 1961, 1982
HpyAV	CCTTC	7 cut(s)	41, 575, 606, 775, 951, 1189, 1240
HpyCH4III	ACNGT	5 cut(s)	209, 1312, 1367, 1397, 2333
HpyCH4IV	ACGT	3 cut(s)	1168, 1848, 2268
HpySE526I	ACGT	3 cut(s)	1168, 1848, 2268
Hsp92I	GRCGYC	1 cut(s)	337
HspAI	GCGC	3 cut(s)	233, 337, 1727
KasI	GGCGCC	1 cut(s)	336
KflI	GGGWCCC	1 cut(s)	1871
KspI	CCGCGG	1 cut(s)	1005
Kzo9I	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
Lsp1109I	GCAGC	7 cut(s)	184, 360, 453, 1042, 1711, 2149, 2207
LweI	GCATC	5 cut(s)	988, 1231, 1424, 1754, 2329
MaeI	CTAG	9 cut(s)	53, 107, 155, 285, 330, 642, 1410, 1673, 1842
MaeII	ACGT	3 cut(s)	1168, 1848, 2268
MaeIII	GTNAC	5 cut(s)	909, 1582, 2020, 2174, 2264
MalI	GATC	7 cut(s)	57, 360, 498, 573, 1438, 1526, 2236
MboI	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
MboII	GAAGA	5 cut(s)	430, 853, 931, 1273, 2301
MfeI	CAATTG	2 cut(s)	1440, 1647
MflI	RGATCY	2 cut(s)	55, 358
MhlI	GDGCHC	5 cut(s)	658, 1146, 1984, 2008, 2290
MlsI	TGGCCA	1 cut(s)	1562
MluCI	AATT	9 cut(s)	211, 504, 627, 687, 799, 870, 1440, 1647, 2044
MluNI	TGGCCA	1 cut(s)	1562
Mly113I	GGCGCC	1 cut(s)	337
MlyI	GAGTC	3 cut(s)	167, 380, 1455
MmeI	TCCRAC	3 cut(s)	240, 354, 1250
Mox20I	TGGCCA	1 cut(s)	1562
MscI	TGGCCA	1 cut(s)	1562
MseI	TTAA	3 cut(s)	636, 705, 723
MslI	CAYNNNNRTG	1 cut(s)	776
Msp20I	TGGCCA	1 cut(s)	1562
MspA1I	CMGCKG	4 cut(s)	197, 253, 466, 1004
MunI	CAATTG	2 cut(s)	1440, 1647
Mva1269I	GAATGC	2 cut(s)	549, 1834
MvaI	CCWGG	8 cut(s)	589, 1359, 1428, 1734, 1926, 2001, 2058, 2232
MvnI	CGCG	2 cut(s)	430, 1004
NarI	GGCGCC	1 cut(s)	337
NciI	CCSGG	5 cut(s)	731, 792, 1140, 1766, 1956
NcoI	CCATGG	1 cut(s)	2273
NdeI	CATATG	1 cut(s)	1965
NdeII	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
NmeAIII	GCCGAG	1 cut(s)	849
NmuCI	GTSAC	2 cut(s)	909, 2020
NspI	RCATGY	1 cut(s)	965
PaqCI	CACCTGC	1 cut(s)	1802
PcsI	WCGNNNNNNNCGW	1 cut(s)	605
PctI	GAATGC	2 cut(s)	549, 1834
PfeI	GAWTC	5 cut(s)	47, 116, 184, 460, 1319
PfoI	TCCNGGA	3 cut(s)	790, 1732, 2056
PinAI	ACCGGT	1 cut(s)	2017
PleI	GAGTC	3 cut(s)	167, 379, 1454
PluTI	GGCGCC	1 cut(s)	340
PpsI	GAGTC	3 cut(s)	167, 379, 1454
Ppu21I	YACGTR	2 cut(s)	1169, 1849
PpuMI	RGGWCCY	1 cut(s)	1871
PshBI	ATTAAT	1 cut(s)	636
Psp1406I	AACGTT	1 cut(s)	2268
Psp5II	RGGWCCY	1 cut(s)	1871
Psp6I	CCWGG	8 cut(s)	587, 1357, 1426, 1732, 1924, 1999, 2056, 2230
PspFI	CCCAGC	3 cut(s)	715, 1773, 1797
PspGI	CCWGG	8 cut(s)	587, 1357, 1426, 1732, 1924, 1999, 2056, 2230
PspPPI	RGGWCCY	1 cut(s)	1871
PsrI	GAACNNNNNNTAC	2 cut(s)	2339, 2371
PstI	CTGCAG	1 cut(s)	100
PsuI	RGATCY	2 cut(s)	55, 358
PvuII	CAGCTG	1 cut(s)	466
RsaI	GTAC	8 cut(s)	1171, 1252, 1369, 1399, 1484, 1619, 1855, 2229
RsaNI	GTAC	8 cut(s)	1170, 1251, 1368, 1398, 1483, 1618, 1854, 2228
RseI	CAYNNNNRTG	1 cut(s)	776
SacII	CCGCGG	1 cut(s)	1005
SaqAI	TTAA	3 cut(s)	636, 705, 723
Sau3AI	GATC	7 cut(s)	55, 358, 496, 571, 1436, 1524, 2234
ScaI	AGTACT	1 cut(s)	1399
SchI	GAGTC	3 cut(s)	167, 380, 1455
SduI	GDGCHC	5 cut(s)	658, 1146, 1984, 2008, 2290
SfaNI	GCATC	5 cut(s)	988, 1231, 1424, 1754, 2329
SfcI	CTRYAG	1 cut(s)	96
SfoI	GGCGCC	1 cut(s)	338
Sfr303I	CCGCGG	1 cut(s)	1005
SgrBI	CCGCGG	1 cut(s)	1005
SinI	GGWCC	4 cut(s)	1576, 1736, 1871, 2245
SmiMI	CAYNNNNRTG	1 cut(s)	776
SmlI	CTYRAG	2 cut(s)	168, 530
SmoI	CTYRAG	2 cut(s)	168, 530
Sse9I	AATT	9 cut(s)	211, 504, 627, 687, 799, 870, 1440, 1647, 2044
SspDI	GGCGCC	1 cut(s)	336
SspMI	CTAG	9 cut(s)	53, 107, 155, 285, 330, 642, 1410, 1673, 1842
StyI	CCWWGG	3 cut(s)	1385, 1865, 2273
TaaI	ACNGT	5 cut(s)	209, 1312, 1367, 1397, 2333
TaiI	ACGT	3 cut(s)	1171, 1851, 2271
TaqI	TCGA	4 cut(s)	45, 215, 541, 1038
TaqII	GACCGA	1 cut(s)	1593
TasI	AATT	9 cut(s)	211, 504, 627, 687, 799, 870, 1440, 1647, 2044
TatI	WGTACW	3 cut(s)	1367, 1397, 1482
TauI	GCSGC	9 cut(s)	142, 253, 291, 342, 345, 348, 1004, 1007, 1501
TfiI	GAWTC	5 cut(s)	47, 116, 184, 460, 1319
Tru1I	TTAA	3 cut(s)	636, 705, 723
Tru9I	TTAA	3 cut(s)	636, 705, 723
TscAI	CASTG	4 cut(s)	412, 1248, 1411, 1758
TseFI	GTSAC	2 cut(s)	909, 2020
TseI	GCWGC	8 cut(s)	8, 197, 348, 466, 1030, 1724, 2162, 2195
Tsp45I	GTSAC	2 cut(s)	909, 2020
TspDTI	ATGAA	5 cut(s)	260, 466, 794, 1673, 2347
TspGWI	ACGGA	3 cut(s)	282, 641, 1635
TspRI	CASTG	4 cut(s)	412, 1248, 1411, 1758
VneI	GTGCAC	1 cut(s)	1980
VpaK11BI	GGWCC	4 cut(s)	1576, 1736, 1871, 2245
VspI	ATTAAT	1 cut(s)	636
XapI	RAATTY	1 cut(s)	504
XceI	RCATGY	1 cut(s)	965
XspI	CTAG	9 cut(s)	53, 107, 155, 285, 330, 642, 1410, 1673, 1842
ZrmI	AGTACT	1 cut(s)	1399

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.