Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0005164

Physical Location & Seq

Genomic Coordinates Forward (+)

31953045 .. 31958424

Gene Structure 5380 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1251300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGGAGGCTGCTCTTCCATCAAACCCTAACCCTCCTCCTCCAATCGAATCCCTAGATCCTCATCCCCCTCCTTCCGAAACCCTACCCTCCTACGATTTCAATCCCCCTCCTCCTGCGGAAACCCTAGCCTCTGATTCGAATGGCCCGCCTCAACCGCCGCACACTGAAACCCTAGCTTCCGATGCTCAAAACCCCTTCCCAAATCCCCAGATCGCTACTGAGAACGGTACTTCGACCGACGCCTGCAAGAAGCTTGCTATTACGAAGCCGCTGTTGTCGGAAAACGGCTTGACCAACACCAATAGCGGCAACGAAAGGGATGGCTCGGGCGGGGAGGAGGAGACGACCAGCAGGCGCCGCCGCCGCAGCCGGTGGGATCCTCAGCCCGAGTCGGAGAGTCAGAGTGGTGGTGGCGAGACGGGCAGTGGGACGAGGAAGAGGAAGTCGCGGTGGGCGGATGAGGAGCCGAAGGCGATTCAGCTGCCGGATTTCATGGGAGGTATTGAGTTCGATCCAGAAATTCAAGCTTTGAATAGTAGGTTGCTTGAGATTAGTCGAATGCTGTCTTCGGGTTTGCCCTTAGATGATCGTCCCGAAGGGGCTAGGTCTCCTTCGCCGGAACCTATATATGATAACATGGGAATTAGGATTAATACGAGAGAGTTCCGTGCCCGTGAAAGGTTGAACAAAGAGAGGCAGGAAATTATTGCTCAGATTATTAAGAGGAACCCGGCATTTAAGCCCCCGGCTGATTACAGGCCACCCAAGCTTCATAAGAAGCTTTACATACCGATGAAGGAATATCCCGGATACAATTTTATTGGACTTATCATTGGGCCGAGGGGTAATACGCAGAAGAGGATGGAGAAAGAAACTGGTGCAAAAATTGTCATTAGGGGTAAAGGTTCGGTGAAAGAGGGCAGGTCGCAACAGAAGAGGGATTTGAAACCCGACCCTTCAGAGAACGAGGACTTGCATGTTTTGGTTGAGGCAGATACACAAGACGCTCTTGATGCTGCGGCTGGGATGGTGGAGAAACTCTTGCAGCCTGTTGATGAGGTGTTGAATGAGCATAAGAGGCAGCAACTTAGGGAACTTGCGGCTTTGAATGGGACAATAAGGGATGAGGAGTATTGTAGGTTGTGTGGTGAGCCGGGGCACCGTCAGTATGCTTGTCCATCACGTACTTCGACCTTCAAGAGCGATGTTCTTTGTAAGATATGTGGTGATGGTGGGCATCCAACCATTGATTGCCCAGTGAAGGGTACAACTGGGAAGAAAATGGATGATGAGTATCAGAACTTCTTGGCTGAGTTGGGGGGGACAGTTCCTGAATCCGCAACTAAGCAGACCGCTACATTGGCACTTGGCCCAGGCAACAGTACATCAAGCAATCCTCCTTGGGCTAACAGTACTAGCAGCACTAGCAGTGCATCATCACATCCAGGCTTGGGATCAATTGGAGTCAAACCGGTCAAGGACTATGATGAGACAAACTTGTACATTGGATACTTGCCGCCTACCCTTGATGACGATGGTTTAATCCGATTGTTTTCAGCGTTTGGTGATATTGTGATGGCCAAAGTTATCAGGGACCGGGTTACCGGAGCGAGTAAGGGTTATGGTTTTGTGAAGTATGGAGATGTTCAGATGGCTAATAATGCAATTGCAAGCATGAATGGTTATCGCCTAGAGCAGCGAACCATTGCTGTCAGAGTTGCTGGCAAGCCTCCGCAGCCGGCTGGACCCCCAGCATCGGCAATGCCCACATACCCCGTTCCCCAGCCTGTTGCTTCCTATCCATCCCAGCAGTTTACCCCCAGTGGTCCTCTTGGGAATGCACCACCTACTAGCTATGCGGGTGCTCCTGTTCCTTGGGGACCCCCTGTTCCTCCTCCTTATGCTCCTTATGCTCCGCCGCCTCCACCTCCCCCGGGTTCAACCATGTATCCTCCTCCCATGCCGGGTCAACATATGTCTCCTTATGGTGCACGATACCCTCCTCCCCCTGGTGCACCACCTCAACCGATGACTTCAAATGAAGCACAACAGAGTTATCCCCCTCCAGGAGTTCAATCTGAACACAATGTCTCTACCCAATCTCTACAAACTAATTTGTATGGTGGCTCCTCAGCACCAATGCCACCAAACGGCCAACACACTTATCCTGCTGCTTCATATGGTTACTCATCTTACTACAATGCAGTTCCACCGCCTCCACCTCCCCCCGGCCCTGTACCTGGATCAACTGCAGATCAATCTCAGAACATTGGTAACGTTCCATGGGCCACGAGTGCTCCGGTTCCCCCTCCTGCTTCTTCTGCGGAAAAGACAACTTATGGTGCAGATGCAGAGTATGAGAAGTTCATGGCAGAAATGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010467 GO:0016070 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0071704 GO:0090304 GO:0097305 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1 RRM_1 SF1-HH

Protein Analysis

795

Amino Acids

85.43

Weight (kDa)

5.75

Isoelectric Point (pI)

61.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SF1-HH	PF16275	147 - 253	3.9e-25	Splicing factor 1 helix-hairpin domain
KH-I_KHDC4-BBP	PF22675	267 - 349	2.7e-24	KHDC4/BBP-like, KH-domain type I
RRM_1	PF00076	500 - 569	5.6e-18	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51300 AT5G51300 AT5G51300
fragaria_vesca	FvH4_6g31120 FvH4_6g31120 FvH4_7g27230 FvH4_7g27230
malus_domestica	MD01G1181200.v1.1 MD07G1251300.v1.1
prunus_persica	Prupe.2G275100_v2.0.a1
pyrus_communis	pycom01g19120 pycom07g22400
rosa_chinensis	RchiOBHm_Chr1g0374011 RchiOBHm_Chr2g0137831
rosa_laevigata	RLG00000019679 RLG00000026786
rosa_roxburghii	Rroxscaffold_2G00106780 Rroxscaffold_4G00283830
rosa_rugosa	Rorug01G0380300 Rorug02G0346500
rosa_samantha	Rh1AG390700 Rh1BG354800 Rh1CG367900 Rh1DG385500 Rh2AG393200 Rh2BG400800 Rh2CG379900 Rh2DG415800
rosa_wichuraiana	Rw1G034450 Rw2G032210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	912
AccB1I	GGYRCC	2 cut(s)	354, 1158
AccII	CGCG	1 cut(s)	448
AclI	AACGTT	1 cut(s)	2277
AclWI	GGATC	6 cut(s)	50, 371, 384, 506, 1462, 2251
AcoI	YGGCCR	2 cut(s)	1578, 2152
AcsI	RAATTY	1 cut(s)	519
AcuI	CTGAAG	1 cut(s)	942
AcyI	GRCGYC	2 cut(s)	240, 355
AfaI	GTAC	7 cut(s)	229, 1186, 1267, 1384, 1414, 1502, 2238
AgeI	ACCGGT	1 cut(s)	1471
AjnI	CCWGG	5 cut(s)	1372, 1444, 2008, 2065, 2239
AloI	GAACNNNNNNTCC	2 cut(s)	1872, 1904
AluBI	AGCT	7 cut(s)	176, 253, 481, 527, 769, 781, 1854
AluI	AGCT	7 cut(s)	176, 253, 481, 527, 769, 781, 1854
Alw21I	GWGCWC	4 cut(s)	1867, 1993, 2017, 2299
Alw26I	GTCTC	6 cut(s)	335, 410, 612, 1484, 1983, 2095
Alw44I	GTGCAC	2 cut(s)	1989, 2013
AlwI	GGATC	6 cut(s)	50, 371, 384, 506, 1462, 2251
AlwNI	CAGNNNCTG	1 cut(s)	1331
Ama87I	CYCGRG	3 cut(s)	325, 386, 1933
AoxI	GGCC	8 cut(s)	142, 758, 836, 1369, 1578, 2152, 2230, 2286
ApaLI	GTGCAC	2 cut(s)	1989, 2013
ApoI	RAATTY	1 cut(s)	519
AseI	ATTAAT	1 cut(s)	651
AsiGI	ACCGGT	1 cut(s)	1471
AspLEI	GCGC	1 cut(s)	357
AspS9I	GGNCC	9 cut(s)	143, 836, 1370, 1594, 1745, 1826, 1880, 2231, 2286
AsuC2I	CCSGG	9 cut(s)	731, 746, 807, 1155, 1598, 1934, 1935, 1965, 2229
AsuHPI	GGTGA	4 cut(s)	922, 1160, 1238, 1577
AsuII	TTCGAA	1 cut(s)	137
AvaI	CYCGRG	3 cut(s)	325, 386, 1933
AvaII	GGWCC	4 cut(s)	1594, 1745, 1826, 1880
BaeGI	GKGCMC	4 cut(s)	673, 1161, 1993, 2017
BalI	TGGCCA	1 cut(s)	1580
BamHI	GGATCC	1 cut(s)	376
BanI	GGYRCC	2 cut(s)	354, 1158
BarI	GAAGNNNNNNTAC	2 cut(s)	1286, 1318
BauI	CACGAG	1 cut(s)	2290
BbsI	GAAGAC	1 cut(s)	558
Bbv12I	GWGCWC	4 cut(s)	1867, 1993, 2017, 2299
BbvCI	CCTCAGC	2 cut(s)	381, 2131
BbvI	GCAGC	9 cut(s)	378, 468, 1003, 1057, 1093, 1431, 1708, 1747, 2158
BceAI	ACGGC	2 cut(s)	301, 2167
BcgI	CGANNNNNNTGC	2 cut(s)	463, 497
BciT130I	CCWGG	5 cut(s)	1374, 1446, 2010, 2067, 2241
BciVI	GTATCC	3 cut(s)	803, 1502, 1959
BcnI	CCSGG	9 cut(s)	731, 746, 807, 1155, 1598, 1934, 1935, 1965, 2229
BcoDI	GTCTC	6 cut(s)	335, 410, 612, 1484, 1983, 2095
BfaI	CTAG	8 cut(s)	53, 125, 173, 603, 1416, 1425, 1691, 1851
BfmI	CTRYAG	1 cut(s)	2250
BfoI	RGCGCY	1 cut(s)	358
BfuAI	ACCTGC	1 cut(s)	912
BfuI	GTATCC	3 cut(s)	803, 1502, 1959
BmcAI	AGTACT	1 cut(s)	1414
Bme18I	GGWCC	4 cut(s)	1594, 1745, 1826, 1880
BmeT110I	CYCGRG	3 cut(s)	325, 386, 1933
BmgT120I	GGNCC	9 cut(s)	143, 836, 1370, 1594, 1745, 1826, 1880, 2231, 2286
BmrI	ACTGGG	3 cut(s)	1250, 1281, 1815
BmsI	GCATC	6 cut(s)	172, 1003, 1246, 1442, 1763, 2338
BmuI	ACTGGG	3 cut(s)	1250, 1281, 1815
BpiI	GAAGAC	1 cut(s)	558
BpmI	CTGGAG	1 cut(s)	2049
Bpu10I	CCTNAGC	2 cut(s)	381, 2131
Bpu14I	TTCGAA	1 cut(s)	137
BpuEI	CTTGAG	1 cut(s)	566
BpuMI	CCSGG	9 cut(s)	731, 746, 807, 1155, 1598, 1934, 1935, 1965, 2229
BsaAI	YACGTR	1 cut(s)	1184
BsaBI	GATNNNNATC	3 cut(s)	60, 99, 1293
BsaHI	GRCGYC	2 cut(s)	240, 355
BsaI	GGTCTC	1 cut(s)	612
BsaWI	WCCGGW	3 cut(s)	1471, 1604, 2299
BsaXI	ACNNNNNCTCC	2 cut(s)	2281, 2311
Bse118I	RCCGGY	3 cut(s)	369, 1471, 1738
Bse1I	ACTGG	4 cut(s)	880, 1256, 1276, 1821
Bse3DI	GCAATG	2 cut(s)	1704, 1767
Bse8I	GATNNNNATC	3 cut(s)	60, 99, 1293
BseBI	CCWGG	5 cut(s)	1374, 1446, 2010, 2067, 2241
BseJI	GATNNNNATC	3 cut(s)	60, 99, 1293
BseMI	GCAATG	2 cut(s)	1704, 1767
BseMII	CTCAG	6 cut(s)	210, 395, 725, 1302, 2145, 2276
BseNI	ACTGG	4 cut(s)	880, 1256, 1276, 1821
BseSI	GKGCMC	4 cut(s)	673, 1161, 1993, 2017
BseXI	GCAGC	9 cut(s)	378, 468, 1003, 1057, 1093, 1431, 1708, 1747, 2158
BseYI	CCCAGC	4 cut(s)	1022, 1750, 1782, 1806
BsgI	GTGCAG	1 cut(s)	2364
Bsh1236I	CGCG	1 cut(s)	448
Bsh1285I	CGRYCG	1 cut(s)	237
BshFI	GGCC	8 cut(s)	144, 760, 838, 1371, 1580, 2154, 2232, 2288
BshNI	GGYRCC	2 cut(s)	354, 1158
BshTI	ACCGGT	1 cut(s)	1471
BsiEI	CGRYCG	1 cut(s)	237
BsiHKAI	GWGCWC	4 cut(s)	1867, 1993, 2017, 2299
BsiHKCI	CYCGRG	3 cut(s)	325, 386, 1933
BslFI	GGGAC	6 cut(s)	442, 576, 1126, 1336, 1607, 1893
BsmAI	GTCTC	6 cut(s)	335, 410, 612, 1484, 1983, 2095
BsmBI	CGTCTC	2 cut(s)	335, 410
BsmFI	GGGAC	6 cut(s)	442, 576, 1126, 1336, 1607, 1893
BsmI	GAATGC	2 cut(s)	564, 1843
BsnI	GGCC	8 cut(s)	144, 760, 838, 1371, 1580, 2154, 2232, 2288
Bso31I	GGTCTC	1 cut(s)	612
BsoBI	CYCGRG	3 cut(s)	325, 386, 1933
Bsp119I	TTCGAA	1 cut(s)	137
Bsp1286I	GDGCHC	6 cut(s)	673, 1161, 1867, 1993, 2017, 2299
Bsp1407I	TGTACA	1 cut(s)	1500
Bsp143I	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
Bsp19I	CCATGG	1 cut(s)	2282
BspANI	GGCC	8 cut(s)	144, 760, 838, 1371, 1580, 2154, 2232, 2288
BspCNI	CTCAG	6 cut(s)	211, 394, 724, 1303, 2144, 2275
BspFNI	CGCG	1 cut(s)	448
BspMAI	CTGCAG	1 cut(s)	2254
BspMI	ACCTGC	1 cut(s)	912
BspPI	GGATC	6 cut(s)	50, 371, 384, 506, 1462, 2251
BspQI	GCTCTTC	1 cut(s)	18
BspT104I	TTCGAA	1 cut(s)	137
BspT107I	GGYRCC	2 cut(s)	354, 1158
BspTNI	GGTCTC	1 cut(s)	612
BsrDI	GCAATG	2 cut(s)	1704, 1767
BsrFI	RCCGGY	3 cut(s)	369, 1471, 1738
BsrGI	TGTACA	1 cut(s)	1500
BsrI	ACTGG	4 cut(s)	880, 1256, 1276, 1821
BssAI	RCCGGY	3 cut(s)	369, 1471, 1738
BssMI	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
BssNI	GRCGYC	2 cut(s)	240, 355
BssSI	CACGAG	1 cut(s)	2290
BssT1I	CCWWGG	3 cut(s)	1400, 1874, 2282
Bst2BI	CACGAG	1 cut(s)	2290
Bst2UI	CCWGG	5 cut(s)	1374, 1446, 2010, 2067, 2241
Bst4CI	ACNGT	5 cut(s)	227, 1163, 1327, 1382, 1412
Bst6I	CTCTTC	4 cut(s)	18, 431, 851, 929
BstACI	GRCGYC	2 cut(s)	240, 355
BstAUI	TGTACA	1 cut(s)	1500
BstBAI	YACGTR	1 cut(s)	1184
BstBI	TTCGAA	1 cut(s)	137
BstC8I	GCNNGC	8 cut(s)	146, 244, 255, 353, 1672, 1723, 1727, 1740
BstDEI	CTNAG	9 cut(s)	219, 381, 580, 711, 1088, 1311, 1344, 2131, 2262
BstDSI	CCRYGG	1 cut(s)	2282
BstEII	GGTNACC	1 cut(s)	1600
BstFNI	CGCG	1 cut(s)	448
BstH2I	RGCGCY	1 cut(s)	358
BstHHI	GCGC	1 cut(s)	357
BstKTI	GATC	8 cut(s)	58, 213, 379, 514, 589, 1457, 2246, 2257
BstMAI	GTCTC	6 cut(s)	335, 410, 612, 1484, 1983, 2095
BstMBI	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
BstMCI	CGRYCG	1 cut(s)	237
BstNI	CCWGG	5 cut(s)	1374, 1446, 2010, 2067, 2241
BstNSI	RCATGY	1 cut(s)	980
BstPI	GGTNACC	1 cut(s)	1600
BstSFI	CTRYAG	1 cut(s)	2250
BstSLI	GKGCMC	4 cut(s)	673, 1161, 1993, 2017
BstUI	CGCG	1 cut(s)	448
BstV1I	GCAGC	9 cut(s)	378, 468, 1003, 1057, 1093, 1431, 1708, 1747, 2158
BstV2I	GAAGAC	1 cut(s)	558
BstX2I	RGATCY	2 cut(s)	55, 376
BstYI	RGATCY	2 cut(s)	55, 376
BsuI	GTATCC	3 cut(s)	803, 1502, 1959
BsuRI	GGCC	8 cut(s)	144, 760, 838, 1371, 1580, 2154, 2232, 2288
BtgI	CCRYGG	1 cut(s)	2282
BtgZI	GCGATG	1 cut(s)	1218
BtsI	GCAGTG	2 cut(s)	430, 1435
BtsIMutI	CAGTG	5 cut(s)	162, 430, 1263, 1435, 1828
BveI	ACCTGC	1 cut(s)	912
Cac8I	GCNNGC	8 cut(s)	146, 244, 255, 353, 1672, 1723, 1727, 1740
CaiI	CAGNNNCTG	1 cut(s)	1331
CfoI	GCGC	1 cut(s)	357
Cfr10I	RCCGGY	3 cut(s)	369, 1471, 1738
Cfr13I	GGNCC	9 cut(s)	143, 836, 1370, 1594, 1745, 1826, 1880, 2231, 2286
Cfr9I	CCCGGG	1 cut(s)	1933
CseI	GACGC	2 cut(s)	248, 1013
Csp6I	GTAC	7 cut(s)	228, 1185, 1266, 1383, 1413, 1501, 2237
CspAI	ACCGGT	1 cut(s)	1471
CviAII	CATG	8 cut(s)	493, 637, 977, 1675, 1945, 1960, 2283, 2368
CviQI	GTAC	7 cut(s)	228, 1185, 1266, 1383, 1413, 1501, 2237
DdeI	CTNAG	9 cut(s)	219, 381, 580, 711, 1088, 1311, 1344, 2131, 2262
DinI	GGCGCC	1 cut(s)	356
DpnI	GATC	8 cut(s)	57, 212, 378, 513, 588, 1456, 2245, 2256
DpnII	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
EaeI	YGGCCR	2 cut(s)	1578, 2152
Eam1104I	CTCTTC	4 cut(s)	18, 431, 851, 929
EarI	CTCTTC	4 cut(s)	18, 431, 851, 929
EciI	GGCGGA	2 cut(s)	470, 1905
Eco130I	CCWWGG	3 cut(s)	1400, 1874, 2282
Eco31I	GGTCTC	1 cut(s)	612
Eco47I	GGWCC	4 cut(s)	1594, 1745, 1826, 1880
Eco57I	CTGAAG	1 cut(s)	942
Eco88I	CYCGRG	3 cut(s)	325, 386, 1933
Eco91I	GGTNACC	1 cut(s)	1600
EcoO109I	RGGNCCY	1 cut(s)	1880
EcoO65I	GGTNACC	1 cut(s)	1600
EcoRII	CCWGG	5 cut(s)	1372, 1444, 2008, 2065, 2239
EcoT14I	CCWWGG	3 cut(s)	1400, 1874, 2282
EgeI	GGCGCC	1 cut(s)	356
EheI	GGCGCC	1 cut(s)	356
ErhI	CCWWGG	3 cut(s)	1400, 1874, 2282
Esp3I	CGTCTC	2 cut(s)	335, 410
FaeI	CATG	8 cut(s)	496, 640, 980, 1678, 1948, 1963, 2286, 2371
FalI	AAGNNNNNCTT	2 cut(s)	993, 1025
FaqI	GGGAC	6 cut(s)	442, 576, 1126, 1336, 1607, 1893
FatI	CATG	8 cut(s)	492, 636, 976, 1674, 1944, 1959, 2282, 2367
FauI	CCCGC	3 cut(s)	153, 323, 1852
FauNDI	CATATG	2 cut(s)	1974, 2179
FspBI	CTAG	8 cut(s)	53, 125, 173, 603, 1416, 1425, 1691, 1851
GlaI	GCGC	1 cut(s)	356
GsaI	CCCAGC	4 cut(s)	1026, 1754, 1786, 1810
GsuI	CTGGAG	1 cut(s)	2049
HaeII	RGCGCY	1 cut(s)	358
HaeIII	GGCC	8 cut(s)	144, 760, 838, 1371, 1580, 2154, 2232, 2288
HgaI	GACGC	2 cut(s)	248, 1013
HhaI	GCGC	1 cut(s)	357
Hin1I	GRCGYC	2 cut(s)	240, 355
Hin1II	CATG	8 cut(s)	496, 640, 980, 1678, 1948, 1963, 2286, 2371
Hin6I	GCGC	1 cut(s)	355
HinP1I	GCGC	1 cut(s)	355
HincII	GTYRAC	1 cut(s)	1970
HindII	GTYRAC	1 cut(s)	1970
HindIII	AAGCTT	4 cut(s)	251, 525, 767, 779
HinfI	GANTC	7 cut(s)	47, 134, 389, 397, 475, 1334, 1464
HphI	GGTGA	4 cut(s)	922, 1160, 1238, 1577
Hpy166II	GTNNAC	4 cut(s)	1815, 1970, 1991, 2015
Hpy188III	TCNNGA	5 cut(s)	515, 593, 1010, 1198, 1331
Hpy8I	GTNNAC	4 cut(s)	1815, 1970, 1991, 2015
Hpy99I	CGWCG	1 cut(s)	242
HpyAV	CCTTC	9 cut(s)	81, 205, 463, 590, 621, 790, 966, 1204, 1255
HpyCH4III	ACNGT	5 cut(s)	227, 1163, 1327, 1382, 1412
HpyCH4IV	ACGT	2 cut(s)	1183, 2277
HpyF3I	CTNAG	9 cut(s)	219, 381, 580, 711, 1088, 1311, 1344, 2131, 2262
HpySE526I	ACGT	2 cut(s)	1183, 2277
Hsp92I	GRCGYC	2 cut(s)	240, 355
Hsp92II	CATG	8 cut(s)	496, 640, 980, 1678, 1948, 1963, 2286, 2371
HspAI	GCGC	1 cut(s)	355
KasI	GGCGCC	1 cut(s)	354
KflI	GGGWCCC	1 cut(s)	1880
KroI	GCCGGC	1 cut(s)	1738
KroNI	GCCGGC	1 cut(s)	1740
Kzo9I	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
LguI	GCTCTTC	1 cut(s)	18
LmnI	GCTCC	7 cut(s)	463, 1607, 1870, 1909, 1918, 2132, 2302
Lsp1109I	GCAGC	9 cut(s)	378, 468, 1003, 1057, 1093, 1431, 1708, 1747, 2158
LweI	GCATC	6 cut(s)	172, 1003, 1246, 1442, 1763, 2338
MaeI	CTAG	8 cut(s)	53, 125, 173, 603, 1416, 1425, 1691, 1851
MaeII	ACGT	2 cut(s)	1183, 2277
MaeIII	GTNAC	3 cut(s)	1600, 2183, 2273
MalI	GATC	8 cut(s)	57, 212, 378, 513, 588, 1456, 2245, 2256
MboI	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
MboII	GAAGA	7 cut(s)	5, 448, 558, 868, 946, 1288, 2310
MfeI	CAATTG	2 cut(s)	1458, 1665
MflI	RGATCY	2 cut(s)	55, 376
MhlI	GDGCHC	6 cut(s)	673, 1161, 1867, 1993, 2017, 2299
MlsI	TGGCCA	1 cut(s)	1580
MluCI	AATT	8 cut(s)	519, 642, 702, 814, 885, 1458, 1665, 2113
MluNI	TGGCCA	1 cut(s)	1580
Mly113I	GGCGCC	1 cut(s)	355
MlyI	GAGTC	3 cut(s)	398, 406, 1473
MmeI	TCCRAC	3 cut(s)	258, 372, 1265
Mox20I	TGGCCA	1 cut(s)	1580
MroNI	GCCGGC	1 cut(s)	1738
MscI	TGGCCA	1 cut(s)	1580
MseI	TTAA	4 cut(s)	651, 720, 738, 1541
MslI	CAYNNNNRTG	1 cut(s)	791
Msp20I	TGGCCA	1 cut(s)	1580
MspA1I	CMGCKG	2 cut(s)	271, 481
MunI	CAATTG	2 cut(s)	1458, 1665
Mva1269I	GAATGC	2 cut(s)	564, 1843
MvaI	CCWGG	5 cut(s)	1374, 1446, 2010, 2067, 2241
MvnI	CGCG	1 cut(s)	448
NaeI	GCCGGC	1 cut(s)	1740
NarI	GGCGCC	1 cut(s)	355
NciI	CCSGG	9 cut(s)	731, 746, 807, 1155, 1598, 1934, 1935, 1965, 2229
NcoI	CCATGG	1 cut(s)	2282
NdeI	CATATG	2 cut(s)	1974, 2179
NdeII	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
NgoMIV	GCCGGC	1 cut(s)	1738
NlaIII	CATG	8 cut(s)	496, 640, 980, 1678, 1948, 1963, 2286, 2371
NmeAIII	GCCGAG	1 cut(s)	864
NspI	RCATGY	1 cut(s)	980
NspV	TTCGAA	1 cut(s)	137
PciSI	GCTCTTC	1 cut(s)	18
PctI	GAATGC	2 cut(s)	564, 1843
PdiI	GCCGGC	1 cut(s)	1740
PfeI	GAWTC	4 cut(s)	47, 134, 475, 1334
PfoI	TCCNGGA	2 cut(s)	805, 2065
PinAI	ACCGGT	1 cut(s)	1471
PleI	GAGTC	3 cut(s)	397, 405, 1472
PluTI	GGCGCC	1 cut(s)	358
PpsI	GAGTC	3 cut(s)	397, 405, 1472
Ppu21I	YACGTR	1 cut(s)	1184
PpuMI	RGGWCCY	1 cut(s)	1880
PshBI	ATTAAT	1 cut(s)	651
Psp1406I	AACGTT	1 cut(s)	2277
Psp5II	RGGWCCY	1 cut(s)	1880
Psp6I	CCWGG	5 cut(s)	1372, 1444, 2008, 2065, 2239
PspEI	GGTNACC	1 cut(s)	1600
PspFI	CCCAGC	4 cut(s)	1022, 1750, 1782, 1806
PspGI	CCWGG	5 cut(s)	1372, 1444, 2008, 2065, 2239
PspPI	GGNCC	9 cut(s)	143, 836, 1370, 1594, 1745, 1826, 1880, 2231, 2286
PspPPI	RGGWCCY	1 cut(s)	1880
PsrI	GAACNNNNNNTAC	2 cut(s)	2348, 2380
PstI	CTGCAG	1 cut(s)	2254
PstNI	CAGNNNCTG	1 cut(s)	1331
PsuI	RGATCY	2 cut(s)	55, 376
PvuII	CAGCTG	1 cut(s)	481
RsaI	GTAC	7 cut(s)	229, 1186, 1267, 1384, 1414, 1502, 2238
RsaNI	GTAC	7 cut(s)	228, 1185, 1266, 1383, 1413, 1501, 2237
RseI	CAYNNNNRTG	1 cut(s)	791
SapI	GCTCTTC	1 cut(s)	18
SaqAI	TTAA	4 cut(s)	651, 720, 738, 1541
Sau3AI	GATC	8 cut(s)	55, 210, 376, 511, 586, 1454, 2243, 2254
Sau96I	GGNCC	9 cut(s)	143, 836, 1370, 1594, 1745, 1826, 1880, 2231, 2286
ScaI	AGTACT	1 cut(s)	1414
SchI	GAGTC	3 cut(s)	398, 406, 1473
SduI	GDGCHC	6 cut(s)	673, 1161, 1867, 1993, 2017, 2299
SfaNI	GCATC	6 cut(s)	172, 1003, 1246, 1442, 1763, 2338
SfcI	CTRYAG	1 cut(s)	2250
SfoI	GGCGCC	1 cut(s)	356
SfuI	TTCGAA	1 cut(s)	137
SinI	GGWCC	4 cut(s)	1594, 1745, 1826, 1880
SmaI	CCCGGG	1 cut(s)	1935
SmiMI	CAYNNNNRTG	1 cut(s)	791
SmlI	CTYRAG	1 cut(s)	545
SmoI	CTYRAG	1 cut(s)	545
Sse9I	AATT	8 cut(s)	519, 642, 702, 814, 885, 1458, 1665, 2113
SspDI	GGCGCC	1 cut(s)	354
SspMI	CTAG	8 cut(s)	53, 125, 173, 603, 1416, 1425, 1691, 1851
StyI	CCWWGG	3 cut(s)	1400, 1874, 2282
TaaI	ACNGT	5 cut(s)	227, 1163, 1327, 1382, 1412
TaiI	ACGT	2 cut(s)	1186, 2280
TaqI	TCGA	6 cut(s)	45, 137, 233, 510, 556, 1190
TaqII	GACCGA	1 cut(s)	251
TasI	AATT	8 cut(s)	519, 642, 702, 814, 885, 1458, 1665, 2113
TatI	WGTACW	3 cut(s)	1382, 1412, 1500
TfiI	GAWTC	4 cut(s)	47, 134, 475, 1334
Tru1I	TTAA	4 cut(s)	651, 720, 738, 1541
Tru9I	TTAA	4 cut(s)	651, 720, 738, 1541
TscAI	CASTG	5 cut(s)	169, 430, 1263, 1435, 1828
TspDTI	ATGAA	7 cut(s)	481, 761, 809, 1691, 2055, 2166, 2356
TspGWI	ACGGA	1 cut(s)	656
TspMI	CCCGGG	1 cut(s)	1933
TspRI	CASTG	5 cut(s)	169, 430, 1263, 1435, 1828
VneI	GTGCAC	2 cut(s)	1989, 2013
VpaK11BI	GGWCC	4 cut(s)	1594, 1745, 1826, 1880
VspI	ATTAAT	1 cut(s)	651
XapI	RAATTY	1 cut(s)	519
XceI	RCATGY	1 cut(s)	980
XmaI	CCCGGG	1 cut(s)	1933
XspI	CTAG	8 cut(s)	53, 125, 173, 603, 1416, 1425, 1691, 1851
ZrmI	AGTACT	1 cut(s)	1414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.