Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009723

Physical Location & Seq

Genomic Coordinates Reverse (-)

30283576 .. 30287711

Gene Structure 4136 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1208200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGGCTGTGCAGGAAGCAGGGGCATTTTTGCATATAGGCCATTCGGTGATCAATTTGCAAAGGAACGGGGCCTCGAGATTACCTGATGCCATTGTTGTTTCAAGTGCTATTCCCCAAAACAATGCTGAGATTTTGTATGCAAAGTCCGTCGGAGTACCTGTGTACAAGCGAGACCAGTGGTTAGGAAAGCTGACAGAGAGCTATAATCTCATTGCTGTAAGCGGTAGCCATGGAAAGAGTACAACTGCAAGTATGCTTGCTTATGTTCTAGATGCCATGGGTGATAGTCTTACCGCAGTAGTTGGAGCACATGTGCCGCAGTTCTCAGGAGGAAACATTATCTTCGGTGATGGTCGTAATTTTGTCCTGGAGGCTGACGAATACGATTGCTGTTTCCTGGGATTATCACCTTCTATAGCCGTTGTAACAAATTTGGATTGGGAGCATGTTGACATATTTCCAAACGAGGAGGCGGTTAAAGCTACTTTCAGGAAATTCCTGAACCGTATCAGGGTCGGTGGACATCTCATTTTATGTGGGGACAGTCAAGGTGCATTTTCATTGCTGACTGATGGAAAGCGGGCAGGTGGATCCGATAACTGGAGATGTTCTGATAGCTACAACGTAACAACTTATGGAACCACAAGTTCTAATGAATGGCATGCATCATCGATCAGGCCAAATTTAAGGGGTGGTAGTGATTATACACTGTGTCATTGCGGGCACCCTGTGGCAGACATCAGTCTACAGATTCCAGGAGTTCATAACGTCCTTAATTCGTTGGCAGTTATTGCCACTGTGATGGCATTATTCAGTGACCAATTTCAAATCAACAATACAATCAATCTTGTGAGGTTGCACTTAAACAATTTCATAGGCATTTCAAGACGTTTTGAGATGATTGGCGCCGTATGCGGATGCCATATATATGACGATTATGCTCATCATCCAACAGAAGTTAGTGCAGTAATTCAAGCTGCACGCCAGAGGTTCCCTGTCAAGTCTCTTTTGGTCGTGTTCCAGCCTCATACTTACAGCCGTTTAACAGCACTGAAGGATGACTTCACCAATGCTCTCCGCGATGCAGATCAAGTCGTAGTCACAGAGGTGTATGCCGCTAGAGAGAAAGATGTCGAGAATGTTGGCGGAAGAGAGTTAGCTGCTACGATAATTGGCCCGCCATCTGAATACATCCCTTCTCTGGACGATGTTGTAGAGAAGTTAGCATCTCAAATATGCAAGAACCCTCGTCGGGAAATCGTTGTTTTAACACTTGGAGCAGGTAATATAACTACCGTCGGCCCAAAGCTGCTCTATGAATTGAGAAGGAGATTACAAGTGAATTCGCAGCTTGCTCCCCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

455

Amino Acids

49.47

Weight (kDa)

6.24

Isoelectric Point (pI)

38.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase	PF01225	4 - 68	3.7e-07	Mur ligase family, catalytic domain
Mur_ligase_M	PF08245	73 - 266	1.5e-18	Mur ligase middle domain
Mur_ligase_C	PF02875	296 - 428	2.8e-17	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009723)

Species	Orthologous Gene IDs
malus_domestica	MD01G1208200.v1.1 MD07G1278800.v1.1
prunus_persica	Prupe.2G300700_v2.0.a1
pyrus_communis	pycom01g21880
rosa_chinensis	RchiOBHm_Chr1g0378261
rosa_laevigata	RLG00000026454 RLG00000026455
rosa_multiflora	Rmu_sc0006315.1_g000004 Rmu_sc0006315.1_g000010 Rmu_sc0013545.1_g000004
rosa_roxburghii	Rroxscaffold_4G00280540 Rroxscaffold_4G00280550 Rroxscaffold_4G00280600
rosa_rugosa	Rorug01G0407600 Rorug01G0407800
rosa_samantha	Rh1AG421700 Rh1AG422200 Rh1BG386900 Rh1CG400400 Rh1DG418000
rosa_wichuraiana	Rw0G013300 Rw1G037520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	575
Acc36I	ACCTGC	2 cut(s)	575, 1271
AccB1I	GGYRCC	2 cut(s)	723, 905
AccI	GTMKAC	1 cut(s)	745
AccII	CGCG	1 cut(s)	1080
AclWI	GGATC	2 cut(s)	585, 598
AcsI	RAATTY	4 cut(s)	430, 494, 682, 1342
AcuI	CTGAAG	1 cut(s)	1073
AcyI	GRCGYC	1 cut(s)	906
AdeI	CACNNNGTG	1 cut(s)	730
AfaI	GTAC	3 cut(s)	156, 164, 241
AfiI	CCNNNNNNNGG	4 cut(s)	511, 600, 1201, 1252
AflIII	ACRYGT	1 cut(s)	310
AgsI	TTSAA	4 cut(s)	102, 827, 885, 974
AjnI	CCWGG	3 cut(s)	366, 396, 754
AleI	CACNNNNGTG	1 cut(s)	1106
AluBI	AGCT	8 cut(s)	190, 201, 482, 618, 977, 1160, 1309, 1351
AluI	AGCT	8 cut(s)	190, 201, 482, 618, 977, 1160, 1309, 1351
Alw21I	GWGCWC	1 cut(s)	310
Alw26I	GTCTC	2 cut(s)	165, 1008
AlwI	GGATC	2 cut(s)	585, 598
Ama87I	CYCGRG	1 cut(s)	73
AoxI	GGCC	5 cut(s)	37, 69, 677, 1174, 1300
ApeKI	GCWGC	4 cut(s)	977, 1160, 1309, 1348
ApoI	RAATTY	4 cut(s)	430, 494, 682, 1342
AspLEI	GCGC	1 cut(s)	908
AspS9I	GGNCC	3 cut(s)	69, 1175, 1301
AsuHPI	GGTGA	5 cut(s)	58, 293, 359, 399, 1057
AvaI	CYCGRG	1 cut(s)	73
BaeGI	GKGCMC	1 cut(s)	726
BamHI	GGATCC	1 cut(s)	590
BanI	GGYRCC	2 cut(s)	723, 905
Bbv12I	GWGCWC	1 cut(s)	310
BbvI	GCAGC	4 cut(s)	964, 1147, 1296, 1360
BccI	CCATC	4 cut(s)	344, 566, 796, 1189
BceAI	ACGGC	3 cut(s)	404, 893, 1023
BciT130I	CCWGG	3 cut(s)	368, 398, 756
BclI	TGATCA	1 cut(s)	48
BcoDI	GTCTC	2 cut(s)	165, 1008
BfaI	CTAG	2 cut(s)	269, 1119
BfmI	CTRYAG	2 cut(s)	414, 746
BfoI	RGCGCY	1 cut(s)	909
BfuAI	ACCTGC	2 cut(s)	575, 1271
BisI	GCNGC	6 cut(s)	317, 978, 1116, 1161, 1310, 1349
BlsI	GCNGC	6 cut(s)	318, 979, 1117, 1162, 1311, 1350
Bme1390I	CCNGG	3 cut(s)	368, 398, 756
BmeT110I	CYCGRG	1 cut(s)	73
BmgT120I	GGNCC	3 cut(s)	69, 1175, 1301
BmiI	GGNNCC	6 cut(s)	70, 592, 640, 725, 907, 992
BmrFI	CCNGG	3 cut(s)	368, 398, 756
BmsI	GCATC	6 cut(s)	76, 262, 674, 908, 1072, 1235
BoxI	GACNNNNGTC	1 cut(s)	741
BpmI	CTGGAG	2 cut(s)	389, 622
Bsa29I	ATCGAT	1 cut(s)	671
BsaBI	GATNNNNATC	1 cut(s)	1086
BsaHI	GRCGYC	1 cut(s)	906
BsaI	GGTCTC	1 cut(s)	165
BsaJI	CCNNGG	3 cut(s)	229, 276, 397
BsaXI	ACNNNNNCTCC	4 cut(s)	144, 174, 297, 327
Bsc4I	CCNNNNNNNGG	4 cut(s)	511, 600, 1201, 1252
Bse1I	ACTGG	2 cut(s)	175, 605
Bse3DI	GCAATG	3 cut(s)	210, 560, 715
Bse8I	GATNNNNATC	1 cut(s)	1086
BseBI	CCWGG	3 cut(s)	368, 398, 756
BseCI	ATCGAT	1 cut(s)	671
BseDI	CCNNGG	3 cut(s)	229, 276, 397
BseGI	GGATG	4 cut(s)	923, 946, 1063, 1191
BseJI	GATNNNNATC	1 cut(s)	1086
BseLI	CCNNNNNNNGG	4 cut(s)	511, 600, 1201, 1252
BseMI	GCAATG	3 cut(s)	210, 560, 715
BseMII	CTCAG	2 cut(s)	117, 339
BseNI	ACTGG	2 cut(s)	175, 605
BseRI	GAGGAG	1 cut(s)	482
BseSI	GKGCMC	1 cut(s)	726
BseXI	GCAGC	4 cut(s)	964, 1147, 1296, 1360
BsgI	GTGCAG	3 cut(s)	29, 963, 984
Bsh1236I	CGCG	1 cut(s)	1080
BshFI	GGCC	5 cut(s)	39, 71, 679, 1176, 1302
BshNI	GGYRCC	2 cut(s)	723, 905
BshVI	ATCGAT	1 cut(s)	671
BsiHKAI	GWGCWC	1 cut(s)	310
BsiHKCI	CYCGRG	1 cut(s)	73
BslFI	GGGAC	1 cut(s)	554
BslI	CCNNNNNNNGG	4 cut(s)	511, 600, 1201, 1252
BsmAI	GTCTC	2 cut(s)	165, 1008
BsmFI	GGGAC	1 cut(s)	554
BsnI	GGCC	5 cut(s)	39, 71, 679, 1176, 1302
Bso31I	GGTCTC	1 cut(s)	165
BsoBI	CYCGRG	1 cut(s)	73
Bsp1286I	GDGCHC	2 cut(s)	310, 726
Bsp1407I	TGTACA	1 cut(s)	162
Bsp143I	GATC	4 cut(s)	48, 590, 672, 1087
Bsp19I	CCATGG	2 cut(s)	229, 276
BspANI	GGCC	5 cut(s)	39, 71, 679, 1176, 1302
BspCNI	CTCAG	2 cut(s)	118, 338
BspDI	ATCGAT	1 cut(s)	671
BspFNI	CGCG	1 cut(s)	1080
BspLI	GGNNCC	6 cut(s)	70, 592, 640, 725, 907, 992
BspMI	ACCTGC	2 cut(s)	575, 1271
BspPI	GGATC	2 cut(s)	585, 598
BspT107I	GGYRCC	2 cut(s)	723, 905
BspTNI	GGTCTC	1 cut(s)	165
BsrDI	GCAATG	3 cut(s)	210, 560, 715
BsrGI	TGTACA	1 cut(s)	162
BsrI	ACTGG	2 cut(s)	175, 605
BssECI	CCNNGG	3 cut(s)	229, 276, 397
BssMI	GATC	4 cut(s)	48, 590, 672, 1087
BssNI	GRCGYC	1 cut(s)	906
BssT1I	CCWWGG	2 cut(s)	229, 276
Bst2UI	CCWGG	3 cut(s)	368, 398, 756
Bst4CI	ACNGT	5 cut(s)	506, 545, 711, 799, 1297
Bst6I	CTCTTC	1 cut(s)	1144
BstACI	GRCGYC	1 cut(s)	906
BstAPI	GCANNNNNTGC	1 cut(s)	791
BstAUI	TGTACA	1 cut(s)	162
BstC8I	GCNNGC	7 cut(s)	258, 582, 663, 722, 982, 1178, 1353
BstDEI	CTNAG	2 cut(s)	126, 325
BstDSI	CCRYGG	2 cut(s)	229, 276
BstF5I	GGATG	4 cut(s)	923, 946, 1063, 1191
BstFNI	CGCG	1 cut(s)	1080
BstH2I	RGCGCY	1 cut(s)	909
BstHHI	GCGC	1 cut(s)	908
BstKTI	GATC	4 cut(s)	51, 593, 675, 1090
BstMAI	GTCTC	2 cut(s)	165, 1008
BstMBI	GATC	4 cut(s)	48, 590, 672, 1087
BstMWI	GCNNNNNNNGC	3 cut(s)	479, 791, 912
BstNI	CCWGG	3 cut(s)	368, 398, 756
BstNSI	RCATGY	3 cut(s)	314, 449, 665
BstPAI	GACNNNNGTC	1 cut(s)	741
BstSCI	CCNGG	3 cut(s)	366, 396, 754
BstSFI	CTRYAG	2 cut(s)	414, 746
BstSLI	GKGCMC	1 cut(s)	726
BstUI	CGCG	1 cut(s)	1080
BstV1I	GCAGC	4 cut(s)	964, 1147, 1296, 1360
BstX2I	RGATCY	1 cut(s)	590
BstXI	CCANNNNNNTGG	1 cut(s)	802
BstYI	RGATCY	1 cut(s)	590
Bsu15I	ATCGAT	1 cut(s)	671
BsuRI	GGCC	5 cut(s)	39, 71, 679, 1176, 1302
BsuTUI	ATCGAT	1 cut(s)	671
BtgI	CCRYGG	2 cut(s)	229, 276
BtgZI	GCGATG	1 cut(s)	1095
BtsCI	GGATG	4 cut(s)	923, 946, 1063, 1191
BtsIMutI	CAGTG	6 cut(s)	182, 707, 795, 820, 1049, 1360
BveI	ACCTGC	2 cut(s)	575, 1271
Cac8I	GCNNGC	7 cut(s)	258, 582, 663, 722, 982, 1178, 1353
CfoI	GCGC	1 cut(s)	908
Cfr13I	GGNCC	3 cut(s)	69, 1175, 1301
ClaI	ATCGAT	1 cut(s)	671
Csp6I	GTAC	3 cut(s)	155, 163, 240
CviAII	CATG	5 cut(s)	230, 277, 311, 446, 662
CviQI	GTAC	3 cut(s)	155, 163, 240
DdeI	CTNAG	2 cut(s)	126, 325
DinI	GGCGCC	1 cut(s)	907
DpnI	GATC	4 cut(s)	50, 592, 674, 1089
DpnII	GATC	4 cut(s)	48, 590, 672, 1087
DraIII	CACNNNGTG	1 cut(s)	730
Eam1104I	CTCTTC	1 cut(s)	1144
EarI	CTCTTC	1 cut(s)	1144
EciI	GGCGGA	1 cut(s)	1161
Eco130I	CCWWGG	2 cut(s)	229, 276
Eco31I	GGTCTC	1 cut(s)	165
Eco57I	CTGAAG	1 cut(s)	1073
Eco88I	CYCGRG	1 cut(s)	73
EcoO109I	RGGNCCY	1 cut(s)	69
EcoRI	GAATTC	1 cut(s)	1342
EcoRII	CCWGG	3 cut(s)	366, 396, 754
EcoT14I	CCWWGG	2 cut(s)	229, 276
EcoT22I	ATGCAT	1 cut(s)	667
EgeI	GGCGCC	1 cut(s)	907
EheI	GGCGCC	1 cut(s)	907
ErhI	CCWWGG	2 cut(s)	229, 276
FaeI	CATG	5 cut(s)	233, 280, 314, 449, 665
FalI	AAGNNNNNCTT	2 cut(s)	1046, 1078
FaqI	GGGAC	1 cut(s)	554
FatI	CATG	5 cut(s)	229, 276, 310, 445, 661
FauI	CCCGC	3 cut(s)	573, 713, 1185
FbaI	TGATCA	1 cut(s)	48
FblI	GTMKAC	1 cut(s)	745
Fnu4HI	GCNGC	6 cut(s)	317, 978, 1116, 1161, 1310, 1349
FokI	GGATG	4 cut(s)	930, 933, 1070, 1178
Fsp4HI	GCNGC	6 cut(s)	317, 978, 1116, 1161, 1310, 1349
FspBI	CTAG	2 cut(s)	269, 1119
GlaI	GCGC	1 cut(s)	907
GluI	GCNGC	6 cut(s)	317, 978, 1116, 1161, 1310, 1349
GsuI	CTGGAG	2 cut(s)	389, 622
HaeII	RGCGCY	1 cut(s)	909
HaeIII	GGCC	5 cut(s)	39, 71, 679, 1176, 1302
HhaI	GCGC	1 cut(s)	908
Hin1I	GRCGYC	1 cut(s)	906
Hin1II	CATG	5 cut(s)	233, 280, 314, 449, 665
Hin6I	GCGC	1 cut(s)	906
HinP1I	GCGC	1 cut(s)	906
HincII	GTYRAC	1 cut(s)	451
HindII	GTYRAC	1 cut(s)	451
HinfI	GANTC	1 cut(s)	751
HphI	GGTGA	5 cut(s)	58, 293, 359, 399, 1057
Hpy166II	GTNNAC	4 cut(s)	163, 451, 521, 746
Hpy188I	TCNGA	4 cut(s)	152, 595, 613, 1186
Hpy188III	TCNNGA	9 cut(s)	75, 269, 327, 490, 499, 885, 1135, 1202, 1253
Hpy8I	GTNNAC	4 cut(s)	163, 451, 521, 746
Hpy99I	CGWCG	3 cut(s)	152, 1254, 1301
HpyAV	CCTTC	4 cut(s)	420, 1048, 1206, 1320
HpyCH4III	ACNGT	5 cut(s)	506, 545, 711, 799, 1297
HpyCH4IV	ACGT	3 cut(s)	624, 768, 889
HpyF10VI	GCNNNNNNNGC	3 cut(s)	479, 791, 912
HpyF3I	CTNAG	2 cut(s)	126, 325
HpySE526I	ACGT	3 cut(s)	624, 768, 889
Hsp92I	GRCGYC	1 cut(s)	906
Hsp92II	CATG	5 cut(s)	233, 280, 314, 449, 665
HspAI	GCGC	1 cut(s)	906
KasI	GGCGCC	1 cut(s)	905
Ksp22I	TGATCA	1 cut(s)	48
Kzo9I	GATC	4 cut(s)	48, 590, 672, 1087
LmnI	GCTCC	4 cut(s)	305, 442, 1277, 1360
Lsp1109I	GCAGC	4 cut(s)	964, 1147, 1296, 1360
LweI	GCATC	6 cut(s)	76, 262, 674, 908, 1072, 1235
MaeI	CTAG	2 cut(s)	269, 1119
MaeII	ACGT	3 cut(s)	624, 768, 889
MaeIII	GTNAC	4 cut(s)	424, 625, 815, 1099
MalI	GATC	4 cut(s)	50, 592, 674, 1089
MboI	GATC	4 cut(s)	48, 590, 672, 1087
MboII	GAAGA	2 cut(s)	334, 1161
MflI	RGATCY	1 cut(s)	590
MhlI	GDGCHC	2 cut(s)	310, 726
Mly113I	GGCGCC	1 cut(s)	906
MmeI	TCCRAC	3 cut(s)	130, 283, 974
Mph1103I	ATGCAT	1 cut(s)	667
MseI	TTAA	6 cut(s)	477, 686, 774, 863, 1043, 1268
MslI	CAYNNNNRTG	3 cut(s)	800, 927, 1106
MspR9I	CCNGG	3 cut(s)	368, 398, 756
MvaI	CCWGG	3 cut(s)	368, 398, 756
MvnI	CGCG	1 cut(s)	1080
MwoI	GCNNNNNNNGC	3 cut(s)	479, 791, 912
NarI	GGCGCC	1 cut(s)	906
NcoI	CCATGG	2 cut(s)	229, 276
NdeII	GATC	4 cut(s)	48, 590, 672, 1087
NlaIII	CATG	5 cut(s)	233, 280, 314, 449, 665
NlaIV	GGNNCC	6 cut(s)	70, 592, 640, 725, 907, 992
NmuCI	GTSAC	2 cut(s)	815, 1099
NsiI	ATGCAT	1 cut(s)	667
NspI	RCATGY	3 cut(s)	314, 449, 665
OliI	CACNNNNGTG	1 cut(s)	1106
PaeI	GCATGC	1 cut(s)	665
PaeR7I	CTCGAG	1 cut(s)	73
PaqCI	CACCTGC	1 cut(s)	575
PciI	ACATGT	1 cut(s)	310
PfeI	GAWTC	1 cut(s)	751
PfoI	TCCNGGA	2 cut(s)	366, 754
PkrI	GCNGC	6 cut(s)	318, 979, 1117, 1162, 1311, 1350
PluTI	GGCGCC	1 cut(s)	909
PscI	ACATGT	1 cut(s)	310
PshAI	GACNNNNGTC	1 cut(s)	741
Psp6I	CCWGG	3 cut(s)	366, 396, 754
PspGI	CCWGG	3 cut(s)	366, 396, 754
PspN4I	GGNNCC	6 cut(s)	70, 592, 640, 725, 907, 992
PspPI	GGNCC	3 cut(s)	69, 1175, 1301
PsuI	RGATCY	1 cut(s)	590
RsaI	GTAC	3 cut(s)	156, 164, 241
RsaNI	GTAC	3 cut(s)	155, 163, 240
RseI	CAYNNNNRTG	3 cut(s)	800, 927, 1106
SaqAI	TTAA	6 cut(s)	477, 686, 774, 863, 1043, 1268
SatI	GCNGC	6 cut(s)	317, 978, 1116, 1161, 1310, 1349
Sau3AI	GATC	4 cut(s)	48, 590, 672, 1087
Sau96I	GGNCC	3 cut(s)	69, 1175, 1301
ScrFI	CCNGG	3 cut(s)	368, 398, 756
SduI	GDGCHC	2 cut(s)	310, 726
SfaNI	GCATC	6 cut(s)	76, 262, 674, 908, 1072, 1235
SfcI	CTRYAG	2 cut(s)	414, 746
SfoI	GGCGCC	1 cut(s)	907
Sfr274I	CTCGAG	1 cut(s)	73
SlaI	CTCGAG	1 cut(s)	73
SmiMI	CAYNNNNRTG	3 cut(s)	800, 927, 1106
SmlI	CTYRAG	1 cut(s)	73
SmoI	CTYRAG	1 cut(s)	73
SphI	GCATGC	1 cut(s)	665
SspDI	GGCGCC	1 cut(s)	905
SspMI	CTAG	2 cut(s)	269, 1119
StyD4I	CCNGG	3 cut(s)	366, 396, 754
StyI	CCWWGG	2 cut(s)	229, 276
TaaI	ACNGT	5 cut(s)	506, 545, 711, 799, 1297
TaiI	ACGT	3 cut(s)	627, 771, 892
TaqI	TCGA	3 cut(s)	74, 671, 1134
TatI	WGTACW	2 cut(s)	162, 239
TauI	GCSGC	2 cut(s)	319, 1118
TfiI	GAWTC	1 cut(s)	751
Tru1I	TTAA	6 cut(s)	477, 686, 774, 863, 1043, 1268
Tru9I	TTAA	6 cut(s)	477, 686, 774, 863, 1043, 1268
TscAI	CASTG	5 cut(s)	182, 714, 802, 820, 1056
TseFI	GTSAC	2 cut(s)	815, 1099
TseI	GCWGC	4 cut(s)	977, 1160, 1309, 1348
Tsp45I	GTSAC	2 cut(s)	815, 1099
TspDTI	ATGAA	5 cut(s)	549, 669, 752, 862, 1332
TspGWI	ACGGA	1 cut(s)	136
TspRI	CASTG	5 cut(s)	182, 714, 802, 820, 1056
XapI	RAATTY	4 cut(s)	430, 494, 682, 1342
XbaI	TCTAGA	1 cut(s)	268
XceI	RCATGY	3 cut(s)	314, 449, 665
XhoI	CTCGAG	1 cut(s)	73
XmiI	GTMKAC	1 cut(s)	745
XspI	CTAG	2 cut(s)	269, 1119
Zsp2I	ATGCAT	1 cut(s)	667

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.