Rh1AG421700

Mur ligase family, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0009723
Physical Location & Seq
Forward (+)
65150287 .. 65157351
7065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG421700.1

Sequence Viewer

Length: 369 bp
ATGAAATTCCCAGCAAGTTGTCCTTTCAAAACCCTCCATGTTCCCGCCAATTTTACAGCAAAGCCAAAGCATCACGTCCAAAAACTTCTCGGAAAGATCTCCGGCCACTCCAAACCCAACCACATTGGAAATTCCGTTCAAGTCTTGACGAAAAATAGTAGTAGCTTCTCAATCTCGACGACGTTCAAGGACGAGATAGAGCAAGAATTGGAGAATTCGAGCAGCGATAAGAGTTGGATTCACTTAGTGGGAATTGGAGGCTGTGGATTGTCTGCGCTGGCCATGCTTGCACTCAAACATGTTATTAGCAAGCTTGAACGCCGCACTGTTGCCTACCATGAGTCAGGGCATGCATTGTTGGTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

122

Amino Acids

13.3

Weight (kDa)

9.39

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 45, 322
AcoI YGGCCR 2 cut(s) 103, 279
AcsI RAATTY 3 cut(s) 5, 130, 214
AdeI CACNNNGTG 1 cut(s) 247
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 4 cut(s) 28, 140, 187, 317
AjiI CACGTC 1 cut(s) 76
AloI GAACNNNNNNTCC 2 cut(s) 120, 152
AluBI AGCT 2 cut(s) 165, 313
AluI AGCT 2 cut(s) 165, 313
AoxI GGCC 2 cut(s) 103, 279
ApeKI GCWGC 1 cut(s) 222
ApoI RAATTY 3 cut(s) 5, 130, 214
AspLEI GCGC 1 cut(s) 277
BalI TGGCCA 1 cut(s) 281
BbvI GCAGC 1 cut(s) 234
BglII AGATCT 1 cut(s) 96
BisI GCNGC 2 cut(s) 223, 322
BlsI GCNGC 2 cut(s) 224, 323
BmgBI CACGTC 1 cut(s) 76
BmsI GCATC 1 cut(s) 79
BseXI GCAGC 1 cut(s) 234
BseYI CCCAGC 1 cut(s) 10
BshFI GGCC 2 cut(s) 105, 281
BsiSI CCGG 1 cut(s) 102
BsnI GGCC 2 cut(s) 105, 281
Bsp143I GATC 1 cut(s) 96
BspACI CCGC 2 cut(s) 45, 322
BspANI GGCC 2 cut(s) 105, 281
BssMI GATC 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 328
BstC8I GCNNGC 4 cut(s) 279, 288, 311, 351
BstDEI CTNAG 1 cut(s) 244
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 2 cut(s) 283, 287
BstNSI RCATGY 2 cut(s) 302, 353
BstV1I GCAGC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
BsuRI GGCC 2 cut(s) 105, 281
BtrI CACGTC 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 4 cut(s) 279, 288, 311, 351
CfoI GCGC 1 cut(s) 277
CviAII CATG 5 cut(s) 38, 283, 299, 338, 350
CviJI RGCY 6 cut(s) 64, 105, 165, 261, 281, 313
CviKI_1 RGCY 6 cut(s) 64, 105, 165, 261, 281, 313
DdeI CTNAG 1 cut(s) 244
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
DraIII CACNNNGTG 1 cut(s) 247
EaeI YGGCCR 2 cut(s) 103, 279
EcoRI GAATTC 1 cut(s) 214
EcoT22I ATGCAT 1 cut(s) 355
FaeI CATG 5 cut(s) 41, 286, 302, 341, 353
FaiI YATR 5 cut(s) 39, 284, 300, 339, 351
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FatI CATG 5 cut(s) 37, 282, 298, 337, 349
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 223, 322
Fsp4HI GCNGC 2 cut(s) 223, 322
GlaI GCGC 1 cut(s) 276
GluI GCNGC 2 cut(s) 223, 322
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 2 cut(s) 105, 281
HapII CCGG 1 cut(s) 102
HhaI GCGC 1 cut(s) 277
Hin1II CATG 5 cut(s) 41, 286, 302, 341, 353
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 2 cut(s) 238, 341
HpaII CCGG 1 cut(s) 102
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 1 cut(s) 92
Hpy188III TCNNGA 2 cut(s) 145, 175
Hpy8I GTNNAC 1 cut(s) 364
Hpy99I CGWCG 2 cut(s) 181, 184
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 2 cut(s) 75, 182
HpyCH4V TGCA 2 cut(s) 290, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 287
HpyF3I CTNAG 1 cut(s) 244
HpySE526I ACGT 2 cut(s) 75, 182
Hsp92II CATG 5 cut(s) 41, 286, 302, 341, 353
HspAI GCGC 1 cut(s) 275
Kzo9I GATC 1 cut(s) 96
LpnPI CCDG 4 cut(s) 24, 115, 263, 330
Lsp1109I GCAGC 1 cut(s) 234
LweI GCATC 1 cut(s) 79
MaeII ACGT 2 cut(s) 75, 182
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MflI RGATCY 1 cut(s) 96
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 6 cut(s) 5, 49, 130, 206, 214, 252
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 215
MnlI CCTC 2 cut(s) 44, 251
Mox20I TGGCCA 1 cut(s) 281
Mph1103I ATGCAT 1 cut(s) 355
MscI TGGCCA 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 1 cut(s) 102
MwoI GCNNNNNNNGC 2 cut(s) 283, 287
NdeII GATC 1 cut(s) 96
NlaIII CATG 5 cut(s) 41, 286, 302, 341, 353
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 2 cut(s) 302, 353
PaeI GCATGC 1 cut(s) 353
PciI ACATGT 1 cut(s) 298
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 2 cut(s) 224, 323
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PscI ACATGT 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 10
PsuI RGATCY 1 cut(s) 96
SatI GCNGC 2 cut(s) 223, 322
Sau3AI GATC 1 cut(s) 96
SchI GAGTC 1 cut(s) 350
SetI ASST 4 cut(s) 78, 167, 185, 315
SfaNI GCATC 1 cut(s) 79
SphI GCATGC 1 cut(s) 353
Sse9I AATT 6 cut(s) 5, 49, 130, 206, 214, 252
SsiI CCGC 2 cut(s) 45, 322
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 2 cut(s) 78, 185
TaqI TCGA 2 cut(s) 176, 218
TasI AATT 6 cut(s) 5, 49, 130, 206, 214, 252
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 1 cut(s) 238
TscAI CASTG 1 cut(s) 331
TseI GCWGC 1 cut(s) 222
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 331
XapI RAATTY 3 cut(s) 5, 130, 214
XceI RCATGY 2 cut(s) 302, 353
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.