Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0009723

Physical Location & Seq

Genomic Coordinates Reverse (-)

64815369 .. 64820109

Gene Structure 4741 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ60169

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGTATGATAAGAATTATTATTTAATACTTACAGGAAAGAGTACAACTGCAAGTATGCTTGCTTATGTTCTAGACGCCATGGGTGATAGTCTTACAGCAGTAGTTGGAGCTCACATGCCACAGTTCTCGGGTGGAAACATTATCTTGGGAGATGGTCAAAATTTTGTGCTAGAGGCTGATGAATATGATGGTTGCTTCCTGGGACTATCACCTTATATAGCTGTTGTAACAAATCTGGATAGGGAGCATGTTGATATTTTCCAAAACGAGGAAGCAGTTAAAGCTACTTTCCATAAATTCCTGAATCTAATCAGGGTGGGTAGACATCTCATTTTATGTGGGGATAGGTACACTGCTCTACTATATTTTAATTTTTGGTGCAGTAGATTGCGGCATTCTAGTAGTGGTGCCTATTCCTTGCTGACTGATGTAAAGCAAGCAATTGGATCAGATACTTTGAGCGGTCTAATGCATTCTCCGCTAGAAAAATGTTGCATTGGCTACATCATAACAACTTATGGAACTACAAGTTCTAATGAATGGAGTGCATCATCAATTAGGCCAAATTTAAAGGTGCCACGACGGCGTGGCCCCCCTCCGTCATATCTCTGGCAAGCTGCACAAGAAGGTAAATTCCAAGTCTCAAATCGGGAGGCCAAGAGTCGTGAAACCGCCTACACCGCTCCGGCCTGCAATAAATTCAGAGAAGCAAAGCCGTTGAGCAAGTTGTGCTTGGAGAACTCCGACGAGATGGAGTCCCCATCGGTGTCCTGTCATTGGGGGCGCTCAGTGGCAGACATCAGTCTACAGGTTCCAGGAGTTCATAATGTCCTAAATTCACTAGCAGTTATTGCCACTGTGATTGCATTATACAATAACCAACGTGAAATTAACAATACAATAAATGGTTTGAGGTTGCATTTGAACAATTTTATAGGCGTTTCAAGACGTTTTGAAATGATTGGCACAATATGTGGATGCGATATATATGATGATTATGCCCATCATCCAATGGAAGTTCAAGCAGTTATTCAAGCTGCCTGCCAGAAGTTCCCTATCAAGTCTCTTTTAGTGGTATTTCAGAATCATACTTACAGTCGTTTAGCAGCACTGAAGGATGATTTTGCCATTGCCCTCTGTGGTGCAGATCAAGTTGTAGTTACAGAGGTTTATGCTGCTAGAGAAATAGATGTTGGGAACGTTGGTGGAAGGGAGTTAGCTGCTTCTGTAATCGCCCGCCATGTGAATACATCCCTTCTCTGGGTGATGTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

424

Amino Acids

46.96

Weight (kDa)

6.5

Isoelectric Point (pI)

37.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase_M	PF08245	12 - 117	7.3e-09	Mur ligase middle domain
Mur_ligase_C	PF02875	316 - 394	3.2e-11	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009723)

Species	Orthologous Gene IDs
malus_domestica	MD01G1208200.v1.1 MD07G1278800.v1.1
prunus_persica	Prupe.2G300700_v2.0.a1
pyrus_communis	pycom01g21880
rosa_chinensis	RchiOBHm_Chr1g0378261
rosa_laevigata	RLG00000026454 RLG00000026455
rosa_multiflora	Rmu_sc0006315.1_g000004 Rmu_sc0006315.1_g000010 Rmu_sc0013545.1_g000004
rosa_roxburghii	Rroxscaffold_4G00280540 Rroxscaffold_4G00280550 Rroxscaffold_4G00280600
rosa_rugosa	Rorug01G0407600 Rorug01G0407800
rosa_samantha	Rh1AG421700 Rh1AG422200 Rh1BG386900 Rh1CG400400 Rh1DG418000
rosa_wichuraiana	Rw0G013300 Rw1G037520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	407, 574
AccBSI	CCGCTC	2 cut(s)	462, 683
AccI	GTMKAC	2 cut(s)	322, 805
AciI	CCGC	6 cut(s)	391, 462, 479, 672, 681, 1237
AclI	AACGTT	1 cut(s)	1200
AclWI	GGATC	1 cut(s)	454
AcsI	RAATTY	6 cut(s)	160, 296, 565, 632, 698, 835
AcuI	CTGAAG	1 cut(s)	1133
AcyI	GRCGYC	1 cut(s)	75
AfaI	GTAC	2 cut(s)	43, 350
AfiI	CCNNNNNNNGG	4 cut(s)	268, 777, 1260, 1261
AgsI	TTSAA	5 cut(s)	925, 945, 956, 1022, 1034
AjnI	CCWGG	2 cut(s)	198, 814
AjuI	GAANNNNNNNTTGG	2 cut(s)	1176, 1208
AluBI	AGCT	6 cut(s)	110, 221, 284, 617, 1037, 1220
AluI	AGCT	6 cut(s)	110, 221, 284, 617, 1037, 1220
Alw21I	GWGCWC	1 cut(s)	112
Alw26I	GTCTC	2 cut(s)	646, 1068
AlwI	GGATC	1 cut(s)	454
Ama87I	CYCGRG	1 cut(s)	127
AoxI	GGCC	4 cut(s)	560, 589, 654, 687
ApeKI	GCWGC	5 cut(s)	617, 1037, 1106, 1175, 1220
ApoI	RAATTY	6 cut(s)	160, 296, 565, 632, 698, 835
AspLEI	GCGC	1 cut(s)	786
AspS9I	GGNCC	1 cut(s)	590
AsuHPI	GGTGA	2 cut(s)	95, 201
AvaI	CYCGRG	1 cut(s)	127
BanI	GGYRCC	2 cut(s)	407, 574
BanII	GRGCYC	1 cut(s)	112
Bbv12I	GWGCWC	1 cut(s)	112
BbvI	GCAGC	5 cut(s)	604, 1024, 1118, 1162, 1207
BccI	CCATC	5 cut(s)	146, 182, 745, 769, 1011
BceAI	ACGGC	2 cut(s)	599, 700
BciT130I	CCWGG	2 cut(s)	200, 816
BcoDI	GTCTC	2 cut(s)	646, 1068
BfaI	CTAG	6 cut(s)	71, 170, 399, 482, 842, 1179
BfmI	CTRYAG	1 cut(s)	806
BfoI	RGCGCY	1 cut(s)	787
BglI	GCCNNNNNGGC	1 cut(s)	583
BisI	GCNGC	6 cut(s)	392, 618, 1038, 1107, 1176, 1221
BlsI	GCNGC	6 cut(s)	393, 619, 1039, 1108, 1177, 1222
Bme1390I	CCNGG	2 cut(s)	200, 816
BmeT110I	CYCGRG	1 cut(s)	127
BmgT120I	GGNCC	1 cut(s)	590
BmiI	GGNNCC	4 cut(s)	409, 576, 592, 813
BmrFI	CCNGG	2 cut(s)	200, 816
BmsI	GCATC	2 cut(s)	557, 968
BoxI	GACNNNNGTC	1 cut(s)	801
BsaHI	GRCGYC	1 cut(s)	75
BsaJI	CCNNGG	2 cut(s)	78, 199
Bsc4I	CCNNNNNNNGG	4 cut(s)	268, 777, 1260, 1261
Bse3DI	GCAATG	1 cut(s)	1128
BseBI	CCWGG	2 cut(s)	200, 816
BseDI	CCNNGG	2 cut(s)	78, 199
BseGI	GGATG	4 cut(s)	983, 1006, 1123, 1250
BseLI	CCNNNNNNNGG	4 cut(s)	268, 777, 1260, 1261
BseMI	GCAATG	1 cut(s)	1128
BseMII	CTCAG	1 cut(s)	801
BseXI	GCAGC	5 cut(s)	604, 1024, 1118, 1162, 1207
BsgI	GTGCAG	3 cut(s)	400, 603, 1164
BshFI	GGCC	4 cut(s)	562, 591, 656, 689
BshNI	GGYRCC	2 cut(s)	407, 574
BsiHKAI	GWGCWC	1 cut(s)	112
BsiHKCI	CYCGRG	1 cut(s)	127
BsiSI	CCGG	1 cut(s)	686
BslFI	GGGAC	2 cut(s)	216, 742
BslI	CCNNNNNNNGG	4 cut(s)	268, 777, 1260, 1261
BsmAI	GTCTC	2 cut(s)	646, 1068
BsmFI	GGGAC	2 cut(s)	216, 742
BsmI	GAATGC	2 cut(s)	394, 472
BsnI	GGCC	4 cut(s)	562, 591, 656, 689
BsoBI	CYCGRG	1 cut(s)	127
Bsp1286I	GDGCHC	1 cut(s)	112
Bsp143I	GATC	2 cut(s)	446, 1147
Bsp19I	CCATGG	1 cut(s)	78
BspACI	CCGC	6 cut(s)	391, 462, 479, 672, 681, 1237
BspANI	GGCC	4 cut(s)	562, 591, 656, 689
BspCNI	CTCAG	1 cut(s)	800
BspLI	GGNNCC	4 cut(s)	409, 576, 592, 813
BspPI	GGATC	1 cut(s)	454
BspT107I	GGYRCC	2 cut(s)	407, 574
BsrBI	CCGCTC	2 cut(s)	462, 683
BsrDI	GCAATG	1 cut(s)	1128
BssECI	CCNNGG	2 cut(s)	78, 199
BssMI	GATC	2 cut(s)	446, 1147
BssNI	GRCGYC	1 cut(s)	75
BssT1I	CCWWGG	1 cut(s)	78
Bst2UI	CCWGG	2 cut(s)	200, 816
Bst4CI	ACNGT	3 cut(s)	123, 859, 1097
BstACI	GRCGYC	1 cut(s)	75
BstAPI	GCANNNNNTGC	2 cut(s)	729, 851
BstC8I	GCNNGC	6 cut(s)	60, 438, 615, 691, 1042, 1237
BstDEI	CTNAG	1 cut(s)	787
BstDSI	CCRYGG	1 cut(s)	78
BstF5I	GGATG	4 cut(s)	983, 1006, 1123, 1250
BstH2I	RGCGCY	1 cut(s)	787
BstHHI	GCGC	1 cut(s)	786
BstKTI	GATC	2 cut(s)	449, 1150
BstMAI	GTCTC	2 cut(s)	646, 1068
BstMBI	GATC	2 cut(s)	446, 1147
BstMWI	GCNNNNNNNGC	6 cut(s)	281, 478, 583, 680, 729, 851
BstNI	CCWGG	2 cut(s)	200, 816
BstNSI	RCATGY	2 cut(s)	118, 251
BstPAI	GACNNNNGTC	1 cut(s)	801
BstSCI	CCNGG	2 cut(s)	198, 814
BstSFI	CTRYAG	1 cut(s)	806
BstV1I	GCAGC	5 cut(s)	604, 1024, 1118, 1162, 1207
BsuRI	GGCC	4 cut(s)	562, 591, 656, 689
BtgI	CCRYGG	1 cut(s)	78
BtsCI	GGATG	4 cut(s)	983, 1006, 1123, 1250
BtsI	GCAGTG	1 cut(s)	351
BtsIMutI	CAGTG	4 cut(s)	351, 795, 855, 1109
Cac8I	GCNNGC	6 cut(s)	60, 438, 615, 691, 1042, 1237
CfoI	GCGC	1 cut(s)	786
Cfr13I	GGNCC	1 cut(s)	590
CseI	GACGC	1 cut(s)	83
Csp6I	GTAC	2 cut(s)	42, 349
CspCI	CAANNNNNGTGG	2 cut(s)	844, 879
CviAII	CATG	4 cut(s)	79, 115, 248, 1241
CviQI	GTAC	2 cut(s)	42, 349
DdeI	CTNAG	1 cut(s)	787
DpnI	GATC	2 cut(s)	448, 1149
DpnII	GATC	2 cut(s)	446, 1147
DraI	TTTAAA	1 cut(s)	570
Ecl136II	GAGCTC	1 cut(s)	110
Eco130I	CCWWGG	1 cut(s)	78
Eco24I	GRGCYC	1 cut(s)	112
Eco53kI	GAGCTC	1 cut(s)	110
Eco57I	CTGAAG	1 cut(s)	1133
Eco88I	CYCGRG	1 cut(s)	127
EcoICRI	GAGCTC	1 cut(s)	110
EcoRII	CCWGG	2 cut(s)	198, 814
EcoT14I	CCWWGG	1 cut(s)	78
EcoT22I	ATGCAT	1 cut(s)	474
EcoT38I	GRGCYC	1 cut(s)	112
ErhI	CCWWGG	1 cut(s)	78
FaeI	CATG	4 cut(s)	82, 118, 251, 1244
FalI	AAGNNNNNCTT	2 cut(s)	716, 748
FaqI	GGGAC	2 cut(s)	216, 742
FatI	CATG	4 cut(s)	78, 114, 247, 1240
FauI	CCCGC	1 cut(s)	1244
FblI	GTMKAC	2 cut(s)	322, 805
Fnu4HI	GCNGC	6 cut(s)	392, 618, 1038, 1107, 1176, 1221
FokI	GGATG	4 cut(s)	990, 993, 1130, 1237
FriOI	GRGCYC	1 cut(s)	112
Fsp4HI	GCNGC	6 cut(s)	392, 618, 1038, 1107, 1176, 1221
FspBI	CTAG	6 cut(s)	71, 170, 399, 482, 842, 1179
GlaI	GCGC	1 cut(s)	785
GluI	GCNGC	6 cut(s)	392, 618, 1038, 1107, 1176, 1221
HaeII	RGCGCY	1 cut(s)	787
HaeIII	GGCC	4 cut(s)	562, 591, 656, 689
HapII	CCGG	1 cut(s)	686
HgaI	GACGC	1 cut(s)	83
HhaI	GCGC	1 cut(s)	786
Hin1I	GRCGYC	1 cut(s)	75
Hin1II	CATG	4 cut(s)	82, 118, 251, 1244
Hin6I	GCGC	1 cut(s)	784
HinP1I	GCGC	1 cut(s)	784
HinfI	GANTC	4 cut(s)	304, 661, 755, 1084
HpaII	CCGG	1 cut(s)	686
HphI	GGTGA	2 cut(s)	95, 201
Hpy166II	GTNNAC	3 cut(s)	323, 351, 806
Hpy188I	TCNGA	4 cut(s)	451, 704, 745, 1083
Hpy188III	TCNNGA	6 cut(s)	71, 236, 301, 650, 665, 945
Hpy8I	GTNNAC	3 cut(s)	323, 351, 806
Hpy99I	CGWCG	2 cut(s)	585, 749
HpyAV	CCTTC	4 cut(s)	620, 1108, 1203, 1265
HpyCH4III	ACNGT	3 cut(s)	123, 859, 1097
HpyCH4IV	ACGT	3 cut(s)	883, 949, 1200
HpyF10VI	GCNNNNNNNGC	6 cut(s)	281, 478, 583, 680, 729, 851
HpyF3I	CTNAG	1 cut(s)	787
HpySE526I	ACGT	3 cut(s)	883, 949, 1200
Hsp92I	GRCGYC	1 cut(s)	75
Hsp92II	CATG	4 cut(s)	82, 118, 251, 1244
HspAI	GCGC	1 cut(s)	784
Kzo9I	GATC	2 cut(s)	446, 1147
LmnI	GCTCC	3 cut(s)	107, 244, 688
Lsp1109I	GCAGC	5 cut(s)	604, 1024, 1118, 1162, 1207
LweI	GCATC	2 cut(s)	557, 968
MaeI	CTAG	6 cut(s)	71, 170, 399, 482, 842, 1179
MaeII	ACGT	3 cut(s)	883, 949, 1200
MaeIII	GTNAC	2 cut(s)	226, 1159
MalI	GATC	2 cut(s)	448, 1149
MbiI	CCGCTC	2 cut(s)	462, 683
MboI	GATC	2 cut(s)	446, 1147
MfeI	CAATTG	1 cut(s)	441
MhlI	GDGCHC	1 cut(s)	112
MlyI	GAGTC	2 cut(s)	670, 764
MmeI	TCCRAC	2 cut(s)	85, 768
MnlI	CCTC	7 cut(s)	166, 262, 606, 646, 906, 1145, 1159
Mph1103I	ATGCAT	1 cut(s)	474
MseI	TTAA	5 cut(s)	23, 279, 369, 569, 891
MspI	CCGG	1 cut(s)	686
MspR9I	CCNGG	2 cut(s)	200, 816
MunI	CAATTG	1 cut(s)	441
Mva1269I	GAATGC	2 cut(s)	394, 472
MvaI	CCWGG	2 cut(s)	200, 816
MwoI	GCNNNNNNNGC	6 cut(s)	281, 478, 583, 680, 729, 851
NcoI	CCATGG	1 cut(s)	78
NdeII	GATC	2 cut(s)	446, 1147
NlaIII	CATG	4 cut(s)	82, 118, 251, 1244
NlaIV	GGNNCC	4 cut(s)	409, 576, 592, 813
NsiI	ATGCAT	1 cut(s)	474
NspI	RCATGY	2 cut(s)	118, 251
PctI	GAATGC	2 cut(s)	394, 472
PfeI	GAWTC	2 cut(s)	304, 1084
PfoI	TCCNGGA	1 cut(s)	814
PkrI	GCNGC	6 cut(s)	393, 619, 1039, 1108, 1177, 1222
PleI	GAGTC	2 cut(s)	669, 763
PpsI	GAGTC	2 cut(s)	669, 763
PshAI	GACNNNNGTC	1 cut(s)	801
Psp124BI	GAGCTC	1 cut(s)	112
Psp1406I	AACGTT	1 cut(s)	1200
Psp6I	CCWGG	2 cut(s)	198, 814
PspGI	CCWGG	2 cut(s)	198, 814
PspN4I	GGNNCC	4 cut(s)	409, 576, 592, 813
PspPI	GGNCC	1 cut(s)	590
RsaI	GTAC	2 cut(s)	43, 350
RsaNI	GTAC	2 cut(s)	42, 349
SacI	GAGCTC	1 cut(s)	112
SaqAI	TTAA	5 cut(s)	23, 279, 369, 569, 891
SatI	GCNGC	6 cut(s)	392, 618, 1038, 1107, 1176, 1221
Sau3AI	GATC	2 cut(s)	446, 1147
Sau96I	GGNCC	1 cut(s)	590
SchI	GAGTC	2 cut(s)	670, 764
ScrFI	CCNGG	2 cut(s)	200, 816
SduI	GDGCHC	1 cut(s)	112
SfaNI	GCATC	2 cut(s)	557, 968
SfcI	CTRYAG	1 cut(s)	806
SsiI	CCGC	6 cut(s)	391, 462, 479, 672, 681, 1237
SspMI	CTAG	6 cut(s)	71, 170, 399, 482, 842, 1179
SstI	GAGCTC	1 cut(s)	112
StyD4I	CCNGG	2 cut(s)	198, 814
StyI	CCWWGG	1 cut(s)	78
TaaI	ACNGT	3 cut(s)	123, 859, 1097
TaiI	ACGT	3 cut(s)	886, 952, 1203
TatI	WGTACW	1 cut(s)	41
TauI	GCSGC	1 cut(s)	394
TfiI	GAWTC	2 cut(s)	304, 1084
Tru1I	TTAA	5 cut(s)	23, 279, 369, 569, 891
Tru9I	TTAA	5 cut(s)	23, 279, 369, 569, 891
TscAI	CASTG	4 cut(s)	358, 795, 862, 1116
TseI	GCWGC	5 cut(s)	617, 1037, 1106, 1175, 1220
TspDTI	ATGAA	3 cut(s)	195, 552, 812
TspGWI	ACGGA	1 cut(s)	588
TspRI	CASTG	4 cut(s)	358, 795, 862, 1116
XapI	RAATTY	6 cut(s)	160, 296, 565, 632, 698, 835
XbaI	TCTAGA	1 cut(s)	70
XceI	RCATGY	2 cut(s)	118, 251
XmiI	GTMKAC	2 cut(s)	322, 805
XspI	CTAG	6 cut(s)	71, 170, 399, 482, 842, 1179
Zsp2I	ATGCAT	1 cut(s)	474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.