RLG00000026454

Mur ligase family, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009723
Physical Location & Seq
Forward (+)
3152472 .. 3154730
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026454

Sequence Viewer

Length: 543 bp
ATGCAGAGGAGATGTGCGTCGAGATTACCTGACGCCGTTGTTGTTTCGAGTGCTATTCCCCAAGACAATGTGGAGATTTTGTATGCAAAGTCGAGGGTGAAAATTGCTTTGAGTTTTGCTGGTAGAAAATGTGATAGGGGGTACAAGCGAGACCATTGGTTAGGACAGCTGACACAGGGCTACAATCTTATTGCTGTAAGCGGTAGCCATGGAATGAGTACAACTGCAAGTATGCTTGCTTATGTTCTAGACGCCATGGGTGATAGTCTCACAGCCGTAGTTGGAGCTCACGTGCCACAGTTCTTGGGTGGAAACATTATCTTGGGAGATGGTCAAAATTTTGTGCTAGAGGCTGATGAATATGATGGTTGCTTCCTGGGACTATCACCTTATATAGCTGTTGTAACAAATCTGGATTGGGAGCACGTTGATATTTTCCAAAACGAGGAAGCAGTTAAAGCTACTTTCCATAAATTCCTGAATCTAATCAGGGTGGGTGGACATCTCATTTTATGTGGCGATAGGGTGCCTATTCCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

181

Amino Acids

19.62

Weight (kDa)

6.03

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_M PF08245 66 - 174 1.6e-10 Mur ligase middle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 526
AciI CCGC 1 cut(s) 201
AcsI RAATTY 2 cut(s) 337, 473
AcvI CACGTG 1 cut(s) 292
AcyI GRCGYC 2 cut(s) 33, 252
AfaI GTAC 2 cut(s) 143, 220
AfiI CCNNNNNNNGG 1 cut(s) 445
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 4 cut(s) 169, 287, 398, 461
AluI AGCT 4 cut(s) 169, 287, 398, 461
Alw21I GWGCWC 2 cut(s) 289, 426
Alw26I GTCTC 2 cut(s) 144, 272
ApoI RAATTY 2 cut(s) 337, 473
AsuHPI GGTGA 3 cut(s) 109, 272, 378
BanI GGYRCC 1 cut(s) 526
BanII GRGCYC 1 cut(s) 289
BbrPI CACGTG 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 289, 426
BccI CCATC 2 cut(s) 323, 359
BceAI ACGGC 2 cut(s) 20, 260
BciT130I CCWGG 1 cut(s) 377
BcoDI GTCTC 2 cut(s) 144, 272
BfaI CTAG 2 cut(s) 248, 347
Bme1390I CCNGG 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 528
BmrFI CCNGG 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 292
BsaHI GRCGYC 2 cut(s) 33, 252
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 208, 255, 376
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 3 cut(s) 208, 255, 376
BseLI CCNNNNNNNGG 1 cut(s) 445
BseRI GAGGAG 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 526
BsiHKAI GWGCWC 2 cut(s) 289, 426
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 1 cut(s) 445
BsmAI GTCTC 2 cut(s) 144, 272
BsmFI GGGAC 1 cut(s) 393
Bso31I GGTCTC 1 cut(s) 144
Bsp1286I GDGCHC 2 cut(s) 289, 426
Bsp19I CCATGG 2 cut(s) 208, 255
BspACI CCGC 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 528
BspT107I GGYRCC 1 cut(s) 526
BspTNI GGTCTC 1 cut(s) 144
BssECI CCNNGG 3 cut(s) 208, 255, 376
BssNI GRCGYC 2 cut(s) 33, 252
BssT1I CCWWGG 2 cut(s) 208, 255
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 300
BstACI GRCGYC 2 cut(s) 33, 252
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 237
BstDSI CCRYGG 2 cut(s) 208, 255
BstMAI GTCTC 2 cut(s) 144, 272
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 375
BtgI CCRYGG 2 cut(s) 208, 255
Cac8I GCNNGC 1 cut(s) 237
CseI GACGC 3 cut(s) 6, 41, 260
Csp6I GTAC 2 cut(s) 142, 219
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CviAII CATG 2 cut(s) 209, 256
CviJI RGCY 8 cut(s) 169, 180, 207, 275, 287, 353, 398, 461
CviKI_1 RGCY 8 cut(s) 169, 180, 207, 275, 287, 353, 398, 461
CviQI GTAC 2 cut(s) 142, 219
Ecl136II GAGCTC 1 cut(s) 287
Eco130I CCWWGG 2 cut(s) 208, 255
Eco24I GRGCYC 1 cut(s) 289
Eco31I GGTCTC 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 287
Eco72I CACGTG 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 2 cut(s) 208, 255
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 2 cut(s) 208, 255
FaeI CATG 2 cut(s) 212, 259
FaqI GGGAC 1 cut(s) 393
FatI CATG 2 cut(s) 208, 255
FriOI GRGCYC 1 cut(s) 289
FspBI CTAG 2 cut(s) 248, 347
HgaI GACGC 3 cut(s) 6, 41, 260
Hin1I GRCGYC 2 cut(s) 33, 252
Hin1II CATG 2 cut(s) 212, 259
HinfI GANTC 1 cut(s) 481
HphI GGTGA 3 cut(s) 109, 272, 378
Hpy166II GTNNAC 1 cut(s) 500
Hpy188III TCNNGA 4 cut(s) 21, 248, 413, 478
Hpy8I GTNNAC 1 cut(s) 500
Hpy99I CGWCG 1 cut(s) 22
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4IV ACGT 2 cut(s) 291, 426
HpyCH4V TGCA 3 cut(s) 4, 86, 227
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpySE526I ACGT 2 cut(s) 291, 426
Hsp92I GRCGYC 2 cut(s) 33, 252
Hsp92II CATG 2 cut(s) 212, 259
LmnI GCTCC 2 cut(s) 284, 421
LpnPI CCDG 8 cut(s) 42, 105, 161, 362, 389, 398, 475, 491
MaeI CTAG 2 cut(s) 248, 347
MaeII ACGT 2 cut(s) 291, 426
MaeIII GTNAC 1 cut(s) 403
MhlI GDGCHC 2 cut(s) 289, 426
MluCI AATT 3 cut(s) 102, 337, 473
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 3 cut(s) 87, 343, 439
MseI TTAA 1 cut(s) 456
MspA1I CMGCKG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 458
NcoI CCATGG 2 cut(s) 208, 255
NlaIII CATG 2 cut(s) 212, 259
NlaIV GGNNCC 1 cut(s) 528
PfeI GAWTC 1 cut(s) 481
PmaCI CACGTG 1 cut(s) 292
PmlI CACGTG 1 cut(s) 292
Ppu21I YACGTR 1 cut(s) 292
Psp124BI GAGCTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 375
PspCI CACGTG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 528
PvuII CAGCTG 1 cut(s) 169
RsaI GTAC 2 cut(s) 143, 220
RsaNI GTAC 2 cut(s) 142, 219
SacI GAGCTC 1 cut(s) 289
SaqAI TTAA 1 cut(s) 456
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 2 cut(s) 289, 426
SetI ASST 8 cut(s) 31, 171, 289, 294, 391, 400, 429, 463
Sse9I AATT 3 cut(s) 102, 337, 473
SsiI CCGC 1 cut(s) 201
SspMI CTAG 2 cut(s) 248, 347
SstI GAGCTC 1 cut(s) 289
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 2 cut(s) 208, 255
TaaI ACNGT 1 cut(s) 300
TaiI ACGT 2 cut(s) 294, 429
TaqI TCGA 3 cut(s) 20, 47, 92
TasI AATT 3 cut(s) 102, 337, 473
TatI WGTACW 1 cut(s) 218
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TspDTI ATGAA 1 cut(s) 372
XapI RAATTY 2 cut(s) 337, 473
XbaI TCTAGA 1 cut(s) 247
XspI CTAG 2 cut(s) 248, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.