Rw0G013300

Mur ligase family, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00597
OG0009723
Physical Location & Seq
Reverse (-)
12 .. 25239
25228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G013300.1

Sequence Viewer

Length: 1032 bp
ATGATATTCCCGGCAATTTGTCTGTCCAAAACCCTCCATTTTCCCGCCAATTTTATAGCGAAGCCAAAGCTTCACGTCCAAAAACTTCTCAGAAAGATCTCTGGCCACTCAAAACCCAATCACATTGGAAAAATGATCTCCGGCCACTCCAAACCCAACCACACTGGAATAGCCTCGACGGAAAACAGTAGTAACTTCTCAATCTCGACGAGTTTCAAGGACGAGAGAGAGCGAGAATTGGAGAATTCTCGCAACGATAAGAGCTGGATACGTTTTGTCGGCATCGAAGGCTGTGGCTTGTCCGCGCTGGCCATGCTTGCACTCAAACAGCTTCATGGACGCATTGCAGGAAGCCGGGGCGTGTTTGCATATAGGTCATTCAGTGACAAATATGCAAAGAAGATGATGACGAGAATACCTGACGTCGTTGTTTCAAGTGCTATTCCTCAAGACAATGTGGAGATTTTGTATGAAAAGTCGGTTGGAGTTCCCGGCTACAATCTTATTGCTGTAAGCGGTAGCCATGGAAAGAGTACAACTGCAAGTATGCTTGCTTATGTTCTAGACGCCATGGGTGATAGTCTTACAGCAGTAGTTGGAGCTCACATGCCACAGTTCTCGGGTGGAAACATTATCTTGGGAGATGGTCAAAATTTTGTGCTAGAGGCTGATGAATATGATGGTTGCTTCCTGGGACTATCACCTTATATAGCTGTTGTAACAAATCTGGATTGGGAGCATGTTGATATTTTCCAAAACGAGGAAGCAGTTAAAGCTACTTTCCATAAATTCCTGAATCTAATCAGGGTGGGTGGACATCTCATTTTATGTGGGGATAGTCAGGGTGCCTATTCCTTGCTGACTGATGTAAAGCAAGCAATTGGATCAGATACTTTGAGCGGTCTAATGCATTCTCCGCTAGAAAAATGTTGCATTGGCTACATCATAACAACTTATGGAACTACAAGTTCTAATGAATGGAGTGCCTCATCAATTAGGCCAAATTTAAAGGGTGGTAGTGATTATACACTG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

344

Amino Acids

37.32

Weight (kDa)

7.69

Isoelectric Point (pI)

28.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_M PF08245 171 - 282 7.6e-12 Mur ligase middle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 900
AccII CGCG 1 cut(s) 305
AciI CCGC 5 cut(s) 45, 303, 516, 900, 917
AclWI GGATC 1 cut(s) 892
AcoI YGGCCR 3 cut(s) 103, 142, 309
AcsI RAATTY 4 cut(s) 244, 652, 788, 1003
AcyI GRCGYC 2 cut(s) 423, 567
AfaI GTAC 1 cut(s) 535
AfiI CCNNNNNNNGG 1 cut(s) 760
AgsI TTSAA 2 cut(s) 217, 435
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 690
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
AluBI AGCT 6 cut(s) 70, 264, 331, 602, 713, 776
AluI AGCT 6 cut(s) 70, 264, 331, 602, 713, 776
Alw21I GWGCWC 1 cut(s) 604
AlwI GGATC 1 cut(s) 892
Ama87I CYCGRG 1 cut(s) 619
AoxI GGCC 4 cut(s) 103, 142, 309, 998
ApoI RAATTY 4 cut(s) 244, 652, 788, 1003
AspLEI GCGC 1 cut(s) 307
AsuC2I CCSGG 3 cut(s) 11, 356, 492
AsuHPI GGTGA 2 cut(s) 587, 693
AvaI CYCGRG 1 cut(s) 619
BalI TGGCCA 2 cut(s) 105, 311
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 1 cut(s) 604
Bbv12I GWGCWC 1 cut(s) 604
BccI CCATC 2 cut(s) 638, 674
BciT130I CCWGG 1 cut(s) 692
BciVI GTATCC 1 cut(s) 261
BcnI CCSGG 3 cut(s) 11, 356, 492
BfaI CTAG 3 cut(s) 563, 662, 920
BfuI GTATCC 1 cut(s) 261
BglII AGATCT 1 cut(s) 96
Bme1390I CCNGG 4 cut(s) 11, 356, 492, 692
BmeT110I CYCGRG 1 cut(s) 619
BmgBI CACGTC 1 cut(s) 76
BmiI GGNNCC 1 cut(s) 847
BmrFI CCNGG 4 cut(s) 11, 356, 492, 692
BmsI GCATC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 432
BpuMI CCSGG 3 cut(s) 11, 356, 492
BsaHI GRCGYC 2 cut(s) 423, 567
BsaJI CCNNGG 4 cut(s) 355, 523, 570, 691
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 760
Bse1I ACTGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 692
BseDI CCNNGG 4 cut(s) 355, 523, 570, 691
BseLI CCNNNNNNNGG 1 cut(s) 760
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 4 cut(s) 105, 144, 311, 1000
BshNI GGYRCC 1 cut(s) 845
BsiHKAI GWGCWC 1 cut(s) 604
BsiHKCI CYCGRG 1 cut(s) 619
BsiSI CCGG 4 cut(s) 11, 141, 355, 492
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 1 cut(s) 760
BsmFI GGGAC 1 cut(s) 708
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 4 cut(s) 105, 144, 311, 1000
BsoBI CYCGRG 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 604
Bsp143I GATC 3 cut(s) 96, 135, 884
Bsp19I CCATGG 2 cut(s) 523, 570
BspACI CCGC 5 cut(s) 45, 303, 516, 900, 917
BspANI GGCC 4 cut(s) 105, 144, 311, 1000
BspCNI CTCAG 1 cut(s) 102
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 1 cut(s) 847
BspPI GGATC 1 cut(s) 892
BspT107I GGYRCC 1 cut(s) 845
BsrBI CCGCTC 1 cut(s) 900
BsrDI GCAATG 1 cut(s) 342
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 4 cut(s) 355, 523, 570, 691
BssMI GATC 3 cut(s) 96, 135, 884
BssNI GRCGYC 2 cut(s) 423, 567
BssT1I CCWWGG 2 cut(s) 523, 570
Bst2UI CCWGG 1 cut(s) 692
Bst4CI ACNGT 2 cut(s) 188, 615
BstACI GRCGYC 2 cut(s) 423, 567
BstC8I GCNNGC 4 cut(s) 309, 318, 552, 876
BstDEI CTNAG 1 cut(s) 89
BstDSI CCRYGG 2 cut(s) 523, 570
BstFNI CGCG 1 cut(s) 305
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 3 cut(s) 99, 138, 887
BstMBI GATC 3 cut(s) 96, 135, 884
BstMWI GCNNNNNNNGC 5 cut(s) 288, 313, 317, 773, 916
BstNI CCWGG 1 cut(s) 692
BstNSI RCATGY 2 cut(s) 610, 743
BstSCI CCNGG 4 cut(s) 9, 354, 490, 690
BstUI CGCG 1 cut(s) 305
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
BsuI GTATCC 1 cut(s) 261
BsuRI GGCC 4 cut(s) 105, 144, 311, 1000
BtgI CCRYGG 2 cut(s) 523, 570
BtrI CACGTC 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 162, 388, 1028
Cac8I GCNNGC 4 cut(s) 309, 318, 552, 876
CfoI GCGC 1 cut(s) 307
CseI GACGC 2 cut(s) 348, 575
Csp6I GTAC 1 cut(s) 534
CviAII CATG 6 cut(s) 313, 335, 524, 571, 607, 740
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 1 cut(s) 89
DpnI GATC 3 cut(s) 98, 137, 886
DpnII GATC 3 cut(s) 96, 135, 884
DraI TTTAAA 1 cut(s) 1008
EaeI YGGCCR 3 cut(s) 103, 142, 309
Ecl136II GAGCTC 1 cut(s) 602
Eco130I CCWWGG 2 cut(s) 523, 570
Eco24I GRGCYC 1 cut(s) 604
Eco53kI GAGCTC 1 cut(s) 602
Eco88I CYCGRG 1 cut(s) 619
EcoICRI GAGCTC 1 cut(s) 602
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 690
EcoT14I CCWWGG 2 cut(s) 523, 570
EcoT22I ATGCAT 1 cut(s) 912
EcoT38I GRGCYC 1 cut(s) 604
ErhI CCWWGG 2 cut(s) 523, 570
FaeI CATG 6 cut(s) 316, 338, 527, 574, 610, 743
FaqI GGGAC 1 cut(s) 708
FatI CATG 6 cut(s) 312, 334, 523, 570, 606, 739
FauI CCCGC 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 604
FspBI CTAG 3 cut(s) 563, 662, 920
GlaI GCGC 1 cut(s) 306
HaeIII GGCC 4 cut(s) 105, 144, 311, 1000
HapII CCGG 4 cut(s) 11, 141, 355, 492
HgaI GACGC 2 cut(s) 348, 575
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 2 cut(s) 423, 567
Hin1II CATG 6 cut(s) 316, 338, 527, 574, 610, 743
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 1 cut(s) 796
HpaII CCGG 4 cut(s) 11, 141, 355, 492
HphI GGTGA 2 cut(s) 587, 693
Hpy166II GTNNAC 1 cut(s) 815
Hpy188I TCNGA 2 cut(s) 92, 889
Hpy188III TCNNGA 5 cut(s) 205, 449, 563, 728, 793
Hpy8I GTNNAC 1 cut(s) 815
Hpy99I CGWCG 3 cut(s) 181, 211, 428
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 2 cut(s) 188, 615
HpyCH4IV ACGT 3 cut(s) 75, 271, 423
HpyCH4V TGCA 7 cut(s) 320, 347, 368, 395, 542, 910, 933
HpyF10VI GCNNNNNNNGC 5 cut(s) 288, 313, 317, 773, 916
HpyF3I CTNAG 1 cut(s) 89
HpySE526I ACGT 3 cut(s) 75, 271, 423
Hsp92I GRCGYC 2 cut(s) 423, 567
Hsp92II CATG 6 cut(s) 316, 338, 527, 574, 610, 743
HspAI GCGC 1 cut(s) 305
Kzo9I GATC 3 cut(s) 96, 135, 884
LmnI GCTCC 2 cut(s) 599, 736
LweI GCATC 1 cut(s) 291
MaeI CTAG 3 cut(s) 563, 662, 920
MaeII ACGT 3 cut(s) 75, 271, 423
MaeIII GTNAC 3 cut(s) 191, 383, 718
MalI GATC 3 cut(s) 98, 137, 886
MbiI CCGCTC 1 cut(s) 900
MboI GATC 3 cut(s) 96, 135, 884
MboII GAAGA 1 cut(s) 412
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 1 cut(s) 604
MlsI TGGCCA 2 cut(s) 105, 311
MluCI AATT 9 cut(s) 15, 49, 236, 244, 652, 788, 879, 993, 1003
MluNI TGGCCA 2 cut(s) 105, 311
MmeI TCCRAC 2 cut(s) 463, 577
MnlI CCTC 6 cut(s) 44, 184, 456, 658, 754, 997
Mox20I TGGCCA 2 cut(s) 105, 311
Mph1103I ATGCAT 1 cut(s) 912
MscI TGGCCA 2 cut(s) 105, 311
MseI TTAA 2 cut(s) 771, 1007
Msp20I TGGCCA 2 cut(s) 105, 311
MspI CCGG 4 cut(s) 11, 141, 355, 492
MspR9I CCNGG 4 cut(s) 11, 356, 492, 692
MunI CAATTG 1 cut(s) 879
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 1 cut(s) 692
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 5 cut(s) 288, 313, 317, 773, 916
NciI CCSGG 3 cut(s) 11, 356, 492
NcoI CCATGG 2 cut(s) 523, 570
NdeII GATC 3 cut(s) 96, 135, 884
NlaIII CATG 6 cut(s) 316, 338, 527, 574, 610, 743
NlaIV GGNNCC 1 cut(s) 847
NmuCI GTSAC 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 912
NspI RCATGY 2 cut(s) 610, 743
PctI GAATGC 1 cut(s) 910
PfeI GAWTC 1 cut(s) 796
Psp124BI GAGCTC 1 cut(s) 604
Psp6I CCWGG 1 cut(s) 690
PspGI CCWGG 1 cut(s) 690
PspN4I GGNNCC 1 cut(s) 847
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
SacI GAGCTC 1 cut(s) 604
SaqAI TTAA 2 cut(s) 771, 1007
Sau3AI GATC 3 cut(s) 96, 135, 884
ScrFI CCNGG 4 cut(s) 11, 356, 492, 692
SduI GDGCHC 1 cut(s) 604
SfaNI GCATC 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 447
SmoI CTYRAG 1 cut(s) 447
Sse9I AATT 9 cut(s) 15, 49, 236, 244, 652, 788, 879, 993, 1003
SsiI CCGC 5 cut(s) 45, 303, 516, 900, 917
SspMI CTAG 3 cut(s) 563, 662, 920
SstI GAGCTC 1 cut(s) 604
StyD4I CCNGG 4 cut(s) 9, 354, 490, 690
StyI CCWWGG 2 cut(s) 523, 570
TaaI ACNGT 2 cut(s) 188, 615
TaiI ACGT 3 cut(s) 78, 274, 426
TaqI TCGA 3 cut(s) 176, 206, 285
TasI AATT 9 cut(s) 15, 49, 236, 244, 652, 788, 879, 993, 1003
TatI WGTACW 1 cut(s) 533
TfiI GAWTC 1 cut(s) 796
Tru1I TTAA 2 cut(s) 771, 1007
Tru9I TTAA 2 cut(s) 771, 1007
TscAI CASTG 2 cut(s) 169, 388
TseFI GTSAC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 4 cut(s) 323, 486, 687, 990
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 2 cut(s) 169, 388
XapI RAATTY 4 cut(s) 244, 652, 788, 1003
XbaI TCTAGA 1 cut(s) 562
XceI RCATGY 2 cut(s) 610, 743
XspI CTAG 3 cut(s) 563, 662, 920
ZraI GACGTC 1 cut(s) 424
Zsp2I ATGCAT 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.