Rroxscaffold_4G00280600

Mur ligase family, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009723
Physical Location & Seq
Forward (+)
3122872 .. 3125026
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280600.1

Sequence Viewer

Length: 537 bp
ATGGCACCGTTTTACACTGACAATGATATTCCCGGCGTTTTGTCCGTCCAACCCTCCATTTTCCCGCCAATTTTACACCCAAGCCAAAGCTTCACTTCTACACACTTCTCGGAAAGATCTCAGGCCACTCCAAACCCAACCACACTGGAATTTCCTGGACCGAAAACAGTAGTGGCTTCTCAGTCTCGACGAGTTGCAACGACGAAAGAGAGCGAAAATTGGAGAATTCTCGCAACGAAAGGAGTTGGATTCACTTCGTCGGCATCGGAGGCTTTGGCTTGTCCGCGCTGGCCATGCTTGCACTCGAACAGGTACAAGCAAGACCATTGGTTAGGACAGCTGACACAGGGCTACAATCTTATTGCTGTAAGCGGTAGCCATGGAAAGAGTACAACTGCAAGTATGCTTGCTTATGTTCCAGACACCATGGGTGATAGTCTTACAGCAGTAGTTGGAGCTCACATGCCACAGTTCTCGGGTGGAAACATTATCTTGGGAGATGGTCAAAATTTTGTGCTAGAGGTATACTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

178

Amino Acids

19.28

Weight (kDa)

6.28

Isoelectric Point (pI)

53.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 525
AccII CGCG 1 cut(s) 286
AciI CCGC 3 cut(s) 65, 284, 372
AcoI YGGCCR 1 cut(s) 290
AcsI RAATTY 3 cut(s) 149, 225, 508
AfaI GTAC 2 cut(s) 314, 391
AjnI CCWGG 1 cut(s) 154
AluBI AGCT 3 cut(s) 90, 340, 458
AluI AGCT 3 cut(s) 90, 340, 458
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 475
AoxI GGCC 2 cut(s) 123, 290
ApoI RAATTY 3 cut(s) 149, 225, 508
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 475
AvaII GGWCC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 292
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 460
BccI CCATC 1 cut(s) 494
BciT130I CCWGG 1 cut(s) 156
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 1 cut(s) 518
BglII AGATCT 1 cut(s) 116
Bme1390I CCNGG 2 cut(s) 33, 156
Bme18I GGWCC 1 cut(s) 158
BmeT110I CYCGRG 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 33, 156
BmsI GCATC 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 379, 426
Bse1I ACTGG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 379, 426
BseMII CTCAG 2 cut(s) 134, 194
BseNI ACTGG 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 286
BshFI GGCC 2 cut(s) 125, 292
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 475
BsiSI CCGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 189
BsnI GGCC 2 cut(s) 125, 292
BsoBI CYCGRG 1 cut(s) 475
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 1 cut(s) 116
Bsp19I CCATGG 2 cut(s) 379, 426
BspACI CCGC 3 cut(s) 65, 284, 372
BspANI GGCC 2 cut(s) 125, 292
BspCNI CTCAG 2 cut(s) 133, 193
BspFNI CGCG 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 379, 426
BssMI GATC 1 cut(s) 116
BssNAI GTATAC 1 cut(s) 526
BssT1I CCWWGG 2 cut(s) 379, 426
Bst1107I GTATAC 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 3 cut(s) 9, 169, 471
BstC8I GCNNGC 3 cut(s) 290, 299, 408
BstDEI CTNAG 2 cut(s) 120, 180
BstDSI CCRYGG 2 cut(s) 379, 426
BstFNI CGCG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 1 cut(s) 119
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 1 cut(s) 116
BstMWI GCNNNNNNNGC 3 cut(s) 269, 294, 298
BstNI CCWGG 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 2 cut(s) 31, 154
BstUI CGCG 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BstZ17I GTATAC 1 cut(s) 526
BsuRI GGCC 2 cut(s) 125, 292
BtgI CCRYGG 2 cut(s) 379, 426
BtsIMutI CAGTG 2 cut(s) 15, 143
Cac8I GCNNGC 3 cut(s) 290, 299, 408
CfoI GCGC 1 cut(s) 288
Cfr13I GGNCC 1 cut(s) 158
Csp6I GTAC 2 cut(s) 313, 390
CviAII CATG 4 cut(s) 294, 380, 427, 463
CviQI GTAC 2 cut(s) 313, 390
DdeI CTNAG 2 cut(s) 120, 180
DpnI GATC 1 cut(s) 118
DpnII GATC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 2 cut(s) 379, 426
Eco24I GRGCYC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 158
Eco53kI GAGCTC 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 154
EcoT14I CCWWGG 2 cut(s) 379, 426
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 2 cut(s) 379, 426
FaeI CATG 4 cut(s) 297, 383, 430, 466
FaiI YATR 7 cut(s) 295, 381, 404, 414, 428, 464, 526
FalI AAGNNNNNCTT 2 cut(s) 79, 111
FatI CATG 4 cut(s) 293, 379, 426, 462
FauI CCCGC 1 cut(s) 72
FblI GTMKAC 1 cut(s) 525
FriOI GRGCYC 1 cut(s) 460
FspBI CTAG 1 cut(s) 518
GlaI GCGC 1 cut(s) 287
HaeIII GGCC 2 cut(s) 125, 292
HapII CCGG 1 cut(s) 33
HhaI GCGC 1 cut(s) 288
Hin1II CATG 4 cut(s) 297, 383, 430, 466
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 1 cut(s) 249
HpaII CCGG 1 cut(s) 33
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 2 cut(s) 112, 268
Hpy188III TCNNGA 2 cut(s) 186, 419
Hpy8I GTNNAC 1 cut(s) 526
Hpy99I CGWCG 3 cut(s) 192, 205, 262
HpyCH4III ACNGT 3 cut(s) 9, 169, 471
HpyCH4V TGCA 3 cut(s) 197, 301, 398
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 294, 298
HpyF3I CTNAG 2 cut(s) 120, 180
Hsp92II CATG 4 cut(s) 297, 383, 430, 466
HspAI GCGC 1 cut(s) 286
Kzo9I GATC 1 cut(s) 116
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 9 cut(s) 46, 107, 131, 141, 168, 274, 295, 332, 432
LweI GCATC 1 cut(s) 272
MaeI CTAG 1 cut(s) 518
MalI GATC 1 cut(s) 118
MboI GATC 1 cut(s) 116
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 460
MlsI TGGCCA 1 cut(s) 292
MluCI AATT 5 cut(s) 69, 149, 217, 225, 508
MluNI TGGCCA 1 cut(s) 292
MmeI TCCRAC 3 cut(s) 73, 226, 433
MnlI CCTC 3 cut(s) 64, 262, 514
Mox20I TGGCCA 1 cut(s) 292
MscI TGGCCA 1 cut(s) 292
MseI TTAA 1 cut(s) 535
Msp20I TGGCCA 1 cut(s) 292
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 2 cut(s) 33, 156
MvaI CCWGG 1 cut(s) 156
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 3 cut(s) 269, 294, 298
NciI CCSGG 1 cut(s) 33
NcoI CCATGG 2 cut(s) 379, 426
NdeII GATC 1 cut(s) 116
NlaIII CATG 4 cut(s) 297, 383, 430, 466
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 466
PfeI GAWTC 1 cut(s) 249
PfoI TCCNGGA 1 cut(s) 154
Psp124BI GAGCTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 340
RsaI GTAC 2 cut(s) 314, 391
RsaNI GTAC 2 cut(s) 313, 390
SacI GAGCTC 1 cut(s) 460
SaqAI TTAA 1 cut(s) 535
Sau3AI GATC 1 cut(s) 116
Sau96I GGNCC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 33, 156
SduI GDGCHC 1 cut(s) 460
SetI ASST 5 cut(s) 92, 314, 342, 460, 525
SfaNI GCATC 1 cut(s) 272
SinI GGWCC 1 cut(s) 158
Sse9I AATT 5 cut(s) 69, 149, 217, 225, 508
SsiI CCGC 3 cut(s) 65, 284, 372
SspMI CTAG 1 cut(s) 518
SstI GAGCTC 1 cut(s) 460
StyD4I CCNGG 2 cut(s) 31, 154
StyI CCWWGG 2 cut(s) 379, 426
TaaI ACNGT 3 cut(s) 9, 169, 471
TaqI TCGA 2 cut(s) 187, 305
TaqII GACCGA 1 cut(s) 175
TasI AATT 5 cut(s) 69, 149, 217, 225, 508
TatI WGTACW 1 cut(s) 389
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 535
Tru9I TTAA 1 cut(s) 535
TscAI CASTG 2 cut(s) 22, 150
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 2 cut(s) 22, 150
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 3 cut(s) 149, 225, 508
XceI RCATGY 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 525
XspI CTAG 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.