RLG00000026455

Mur ligase family, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009723
Physical Location & Seq
Forward (+)
3154775 .. 3155956
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026455

Sequence Viewer

Length: 645 bp
ATGCATTCTCCTCTAGAAAAATGTTGCATTGGCTACAACATAACAACTTATGGAACTACAAGTTCTAACGAATGGAGTGCATCATCAATTAGGCCATATTTAAAGGGTGGTAGTAGTGATTATACACTGTGTCAGTGGGGGCGCTTAGTGGCAGACATCAGTCTACAGGTTCCAGGAGTTCATAATGTCCTAAATTCACTAGCAGTTATTGCCACTGTGATAGGATTATACAATAACCAACGTGAAATTAACAATACAATAAATGGTTTGAGGTTGCATTTGAACAATTTTACAGGCGTTTCAAGACGTTTTGAGATGATTGGCACAATATGTGGATGCGATATATATGATGATTATGCCCACCCTCCAACAGAAGTTCAAGCAGTTATTCAAGCTGCCCGCCAGAAGTTCCCTATCAAGTCTCTTTTAGTGGTACTTCAGCCTCATACTTACAGTTGTTTAGCAGCACTGAAGGATGATTTTGCCAGTGCCCTCTGTGGTGCAGATCAACTTGTAGTTACAGAGGTTTATGCTGCTAGAGAAATAGATGTTAGGAACGTTGGTGGAAGGGAGTTAGCTGCTTCTGTAATCGGCCGGCCATCTGAATACATCCCTTCTCTGGATGCATTTTGGGGAATCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

215

Amino Acids

23.43

Weight (kDa)

5.93

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_C PF02875 102 - 208 7.2e-13 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 163
AciI CCGC 1 cut(s) 400
AclI AACGTT 1 cut(s) 558
AcoI YGGCCR 2 cut(s) 592, 596
AcsI RAATTY 1 cut(s) 193
AcuI CTGAAG 2 cut(s) 422, 491
AfaI GTAC 1 cut(s) 435
AfiI CCNNNNNNNGG 1 cut(s) 619
AgsI TTSAA 4 cut(s) 283, 303, 380, 392
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 2 cut(s) 395, 578
AluI AGCT 2 cut(s) 395, 578
Alw26I GTCTC 1 cut(s) 426
AoxI GGCC 3 cut(s) 92, 592, 596
ApeKI GCWGC 4 cut(s) 395, 464, 533, 578
ApoI RAATTY 1 cut(s) 193
AspLEI GCGC 1 cut(s) 144
BaeGI GKGCMC 1 cut(s) 493
BbvI GCAGC 4 cut(s) 382, 476, 520, 565
BccI CCATC 1 cut(s) 607
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 3 cut(s) 14, 200, 537
BfmI CTRYAG 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 145
BisI GCNGC 4 cut(s) 396, 465, 534, 579
BlsI GCNGC 4 cut(s) 397, 466, 535, 580
Bme1390I CCNGG 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 89, 326, 613
BoxI GACNNNNGTC 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse118I RCCGGY 1 cut(s) 594
Bse1I ACTGG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 174
BseGI GGATG 4 cut(s) 341, 481, 609, 628
BseLI CCNNNNNNNGG 1 cut(s) 619
BseNI ACTGG 1 cut(s) 486
BseSI GKGCMC 1 cut(s) 493
BseX3I CGGCCG 1 cut(s) 592
BseXI GCAGC 4 cut(s) 382, 476, 520, 565
BsgI GTGCAG 1 cut(s) 522
Bsh1285I CGRYCG 1 cut(s) 595
BshFI GGCC 3 cut(s) 94, 594, 598
BsiEI CGRYCG 1 cut(s) 595
BsiSI CCGG 1 cut(s) 595
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 1 cut(s) 426
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 3 cut(s) 94, 594, 598
Bsp1286I GDGCHC 1 cut(s) 493
Bsp143I GATC 1 cut(s) 505
BspACI CCGC 1 cut(s) 400
BspANI GGCC 3 cut(s) 94, 594, 598
BspLI GGNNCC 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 594
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 594
BssMI GATC 1 cut(s) 505
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 3 cut(s) 129, 217, 455
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 400, 596
BstDEI CTNAG 1 cut(s) 145
BstF5I GGATG 4 cut(s) 341, 481, 609, 628
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 1 cut(s) 508
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 1 cut(s) 505
BstMCI CGRYCG 1 cut(s) 595
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 1 cut(s) 174
BstPAI GACNNNNGTC 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 164
BstSLI GKGCMC 1 cut(s) 493
BstV1I GCAGC 4 cut(s) 382, 476, 520, 565
BstZI CGGCCG 1 cut(s) 592
BsuRI GGCC 3 cut(s) 94, 594, 598
BtsCI GGATG 4 cut(s) 341, 481, 609, 628
BtsIMutI CAGTG 5 cut(s) 125, 140, 213, 467, 493
Cac8I GCNNGC 2 cut(s) 400, 596
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 594
Csp6I GTAC 1 cut(s) 434
CviJI RGCY 7 cut(s) 33, 94, 395, 442, 578, 594, 598
CviKI_1 RGCY 7 cut(s) 33, 94, 395, 442, 578, 594, 598
CviQI GTAC 1 cut(s) 434
DdeI CTNAG 1 cut(s) 145
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
DraI TTTAAA 1 cut(s) 102
EaeI YGGCCR 2 cut(s) 592, 596
EagI CGGCCG 1 cut(s) 592
EclXI CGGCCG 1 cut(s) 592
Eco52I CGGCCG 1 cut(s) 592
Eco57I CTGAAG 2 cut(s) 422, 491
EcoRII CCWGG 1 cut(s) 172
EcoT22I ATGCAT 2 cut(s) 6, 628
FauI CCCGC 1 cut(s) 407
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 4 cut(s) 396, 465, 534, 579
FokI GGATG 4 cut(s) 348, 488, 596, 635
FseI GGCCGGCC 1 cut(s) 598
Fsp4HI GCNGC 4 cut(s) 396, 465, 534, 579
FspBI CTAG 3 cut(s) 14, 200, 537
GlaI GCGC 1 cut(s) 143
GluI GCNGC 4 cut(s) 396, 465, 534, 579
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 3 cut(s) 94, 594, 598
HapII CCGG 1 cut(s) 595
HhaI GCGC 1 cut(s) 144
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HinfI GANTC 1 cut(s) 636
HpaII CCGG 1 cut(s) 595
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 1 cut(s) 604
Hpy188III TCNNGA 3 cut(s) 14, 303, 620
Hpy8I GTNNAC 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 466, 561, 624
HpyCH4III ACNGT 3 cut(s) 129, 217, 455
HpyCH4IV ACGT 3 cut(s) 241, 307, 558
HpyCH4V TGCA 6 cut(s) 4, 27, 80, 277, 503, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 145
HpySE526I ACGT 3 cut(s) 241, 307, 558
HspAI GCGC 1 cut(s) 142
KroI GCCGGC 1 cut(s) 594
KroNI GCCGGC 1 cut(s) 596
Kzo9I GATC 1 cut(s) 505
LpnPI CCDG 8 cut(s) 152, 159, 186, 279, 416, 499, 605, 608
Lsp1109I GCAGC 4 cut(s) 382, 476, 520, 565
LweI GCATC 3 cut(s) 89, 326, 613
MaeI CTAG 3 cut(s) 14, 200, 537
MaeII ACGT 3 cut(s) 241, 307, 558
MaeIII GTNAC 1 cut(s) 517
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 4 cut(s) 87, 193, 246, 286
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 6 cut(s) 21, 264, 375, 453, 503, 517
Mph1103I ATGCAT 2 cut(s) 6, 628
MroNI GCCGGC 1 cut(s) 594
MseI TTAA 2 cut(s) 101, 249
MspI CCGG 1 cut(s) 595
MspR9I CCNGG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 209
NaeI GCCGGC 1 cut(s) 596
NdeII GATC 1 cut(s) 505
NgoMIV GCCGGC 1 cut(s) 594
NlaIV GGNNCC 1 cut(s) 171
NsiI ATGCAT 2 cut(s) 6, 628
PctI GAATGC 1 cut(s) 4
PdiI GCCGGC 1 cut(s) 596
PfeI GAWTC 1 cut(s) 636
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 4 cut(s) 397, 466, 535, 580
PshAI GACNNNNGTC 1 cut(s) 159
Psp1406I AACGTT 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 171
RigI GGCCGGCC 1 cut(s) 598
RsaI GTAC 1 cut(s) 435
RsaNI GTAC 1 cut(s) 434
SaqAI TTAA 2 cut(s) 101, 249
SatI GCNGC 4 cut(s) 396, 465, 534, 579
Sau3AI GATC 1 cut(s) 505
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 493
SetI ASST 8 cut(s) 171, 244, 275, 310, 397, 528, 561, 580
SfaNI GCATC 3 cut(s) 89, 326, 613
SfcI CTRYAG 1 cut(s) 164
Sse9I AATT 4 cut(s) 87, 193, 246, 286
SsiI CCGC 1 cut(s) 400
SspMI CTAG 3 cut(s) 14, 200, 537
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 3 cut(s) 129, 217, 455
TaiI ACGT 3 cut(s) 244, 310, 561
TasI AATT 4 cut(s) 87, 193, 246, 286
TfiI GAWTC 1 cut(s) 636
Tru1I TTAA 2 cut(s) 101, 249
Tru9I TTAA 2 cut(s) 101, 249
TscAI CASTG 5 cut(s) 132, 140, 220, 474, 493
TseI GCWGC 4 cut(s) 395, 464, 533, 578
TspDTI ATGAA 1 cut(s) 170
TspRI CASTG 5 cut(s) 132, 140, 220, 474, 493
XapI RAATTY 1 cut(s) 193
XbaI TCTAGA 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 3 cut(s) 14, 200, 537
Zsp2I ATGCAT 2 cut(s) 6, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.