Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0009723

Physical Location & Seq

Genomic Coordinates Reverse (-)

19700051 .. 19705009

Gene Structure 4959 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g21880.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGAATTGGGCCTTTTGGATATCAACTCAAGTGATTAAAGGCCCATTTTGTAATTTTAAACTATCCGTTTCCATTTCTTCACGATCCAACTTCAAAGCCGTCACTTTCCCGGGAAAAGCACTGTATCACAGTGCTATCACGCCAATGGAATTCCCGGCAATGTCAACGTCCAAACGCCTCCATTTTCCGGCAAATATCATCGGAAAACCGAAGATCGACTTCCAGATATTTGTGAGGGAGAGCTCCGCCCAGTGCAAACGCAATCGCATTGCGAAATCCGTGCAAGCATCGAGTAAAAACAACAGCGGCAGCATCTCAATCTCGCCGGCGTTCACGGACGAGAGCGATCGAGAATCCGATGGTTTGAGAAACGAAAAGGGTTGGATTCACTTTGTGGGGATTGGAGGTTGCGGGTTGTCTGCGCTGGCCATGCTTGCACTCAAACAGGGTTATGAGGTTAGTGGCTCGGATATTGTGTGGAGTAGCTTCATGGATGGGCTGCAGGAAGCAGGGGCATTTTTGCATATAGGCCATTCGGTGACCAATTTGCAAAGGAACGGGGCCCCGAGATTACCTGATGCCATTGTTGTTTCAAGTGCTATTCCCCAAAACAATGCTGAGATTTTGTATGCAAAGTCCGTCGGAGTACCCGTGTACAAGCGAGACCAGTGGTTAGGAAAGCTGACAGAGGGCTATAATCTCGTTGCTGTAAGCGGTAGCCATGGAAAGAGTACAACTGCAAGTATGCTTGCTTATGTTCTAGACGCCATGGGTGATAGTCTTACCGCAGTAGTTGGAGCACATGTGCCGCAGTTCTCAGGAGGAAACATTATCTTTGGTGATGGTCGTAATTTTGTCCTGGAGGCTGATGAATACGATCGCTGTTTCCTGGGATTATCACCTTCTATAGCCGTTGTAACAAATTTGGATTGGGAGCATGTTGATATATTTCCAAACGAGGAGGCGGTTAAAGCTACTTTCAGGAAATTCCTGAACCGTATCAGGGTGGGCGGACATCTCATTTTATGTGGGGATAGTCAAGGTGCATGTTCATTGCTGACTGATGGAAAGCGAGCAATTGGATCCGATAGCTGGAAATGTTCTGATAGCTACAACATAACAACTTATGGAACCGCAAGTTCTAATGAATGGCATGCATCATCAATCAGGCCAAATTTAAGGGGTGGTAGTGATTATACACTGTGTCATTGCGGGCACCCTGTGGCAGACATCAGTCTACAGATTCCAGGAGTTCATAACGTCCTTAATTCGTTGGCAGTTGTTGCCACTGTGATAGCATTATTCAGTGACCAATGTCAAATCAACAATACAATCAATCTTGTGAGGTTGCACTTAAACAATTTCATAGGCATTTCAAGACGTTTTGAGATGATTGGCACCGTGTGCGGATGCCACATATATGACGATTATGCTCATCATCCAACAGAAGTTAGTGCAGTTATTCAAGCTGCACGCCAGAGGTTCCCTGTCAAGTCTCTTTTGGTCGTGTTCCAGCCTCATACTTACAGCCGTTTAACAGCACTGAAGGATGACTTTGCCAATGCTCTCCGCGATGCAGATCAAGTCGTAGTCACAGAGGTGTATGCCGCTAGAGAGAAAGATGTCGAGAATGTTGGCGGAAGGGAGTTAGCTGCTACGATAATTGGCCCGCCATCTGAATACATCCCTTCTCTGGACGATGTTGTAGAGAAGTTAGCATCTCAAATATGCAAGAACCCTCATCGGGAAATCGTAGTTTTTACACTTGGAGCAGGTAATATAACTACCGTCGGCCCAAAGCTGCTCTATGAATTGAGAAGGAGATTACAAGTGAATTCGTCGCTTCATCGTCAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

620

Amino Acids

67.52

Weight (kDa)

7.92

Isoelectric Point (pI)

37.15

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009723)

Species	Orthologous Gene IDs
malus_domestica	MD01G1208200.v1.1 MD07G1278800.v1.1
prunus_persica	Prupe.2G300700_v2.0.a1
pyrus_communis	pycom01g21880
rosa_chinensis	RchiOBHm_Chr1g0378261
rosa_laevigata	RLG00000026454 RLG00000026455
rosa_multiflora	Rmu_sc0006315.1_g000004 Rmu_sc0006315.1_g000010 Rmu_sc0013545.1_g000004
rosa_roxburghii	Rroxscaffold_4G00280540 Rroxscaffold_4G00280550 Rroxscaffold_4G00280600
rosa_rugosa	Rorug01G0407600 Rorug01G0407800
rosa_samantha	Rh1AG421700 Rh1AG422200 Rh1BG386900 Rh1CG400400 Rh1DG418000
rosa_wichuraiana	Rw0G013300 Rw1G037520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1763
AccB1I	GGYRCC	2 cut(s)	1215, 1397
AccI	GTMKAC	1 cut(s)	1237
AccII	CGCG	1 cut(s)	1572
AclWI	GGATC	3 cut(s)	78, 1077, 1090
AcoI	YGGCCR	1 cut(s)	426
AcsI	RAATTY	5 cut(s)	149, 922, 986, 1174, 1834
AcuI	CTGAAG	1 cut(s)	1565
AcyI	GRCGYC	1 cut(s)	765
AdeI	CACNNNGTG	3 cut(s)	394, 1222, 1404
AfaI	GTAC	3 cut(s)	648, 656, 733
AfiI	CCNNNNNNNGG	5 cut(s)	187, 1003, 1092, 1693, 1744
AflIII	ACRYGT	1 cut(s)	802
AgsI	TTSAA	4 cut(s)	94, 594, 1377, 1466
AjnI	CCWGG	3 cut(s)	858, 888, 1246
AleI	CACNNNNGTG	1 cut(s)	1598
AluBI	AGCT	9 cut(s)	243, 486, 682, 974, 1092, 1110, 1469, 1652, 1801
AluI	AGCT	9 cut(s)	243, 486, 682, 974, 1092, 1110, 1469, 1652, 1801
Alw21I	GWGCWC	2 cut(s)	245, 802
Alw26I	GTCTC	2 cut(s)	657, 1500
AlwI	GGATC	3 cut(s)	78, 1077, 1090
Ama87I	CYCGRG	2 cut(s)	109, 565
AoxI	GGCC	8 cut(s)	9, 40, 426, 529, 561, 1169, 1666, 1792
ApaI	GGGCCC	1 cut(s)	565
ApeKI	GCWGC	5 cut(s)	309, 499, 1469, 1652, 1801
ApoI	RAATTY	5 cut(s)	149, 922, 986, 1174, 1834
AspLEI	GCGC	1 cut(s)	424
AspS9I	GGNCC	6 cut(s)	9, 41, 561, 562, 1667, 1793
AsuC2I	CCSGG	3 cut(s)	110, 111, 155
AsuHPI	GGTGA	4 cut(s)	550, 785, 851, 891
AvaI	CYCGRG	2 cut(s)	109, 565
BaeGI	GKGCMC	2 cut(s)	565, 1218
BalI	TGGCCA	1 cut(s)	428
BamHI	GGATCC	1 cut(s)	1082
BanI	GGYRCC	2 cut(s)	1215, 1397
BanII	GRGCYC	2 cut(s)	245, 565
Bbv12I	GWGCWC	2 cut(s)	245, 802
BbvI	GCAGC	5 cut(s)	321, 486, 1456, 1639, 1788
BccI	CCATC	5 cut(s)	353, 488, 836, 1058, 1681
BceAI	ACGGC	3 cut(s)	83, 896, 1515
BcgI	CGANNNNNNTGC	2 cut(s)	262, 296
BciT130I	CCWGG	3 cut(s)	860, 890, 1248
BcnI	CCSGG	3 cut(s)	110, 111, 155
BcoDI	GTCTC	2 cut(s)	657, 1500
BfaI	CTAG	2 cut(s)	761, 1611
BfmI	CTRYAG	3 cut(s)	500, 906, 1238
BfuAI	ACCTGC	1 cut(s)	1763
BisI	GCNGC	8 cut(s)	307, 310, 500, 809, 1470, 1608, 1653, 1802
BlsI	GCNGC	8 cut(s)	308, 311, 501, 810, 1471, 1609, 1654, 1803
Bme1390I	CCNGG	6 cut(s)	110, 111, 155, 860, 890, 1248
BmeT110I	CYCGRG	2 cut(s)	109, 565
BmgT120I	GGNCC	6 cut(s)	9, 41, 561, 562, 1667, 1793
BmiI	GGNNCC	8 cut(s)	562, 563, 564, 1084, 1132, 1217, 1399, 1484
BmrFI	CCNGG	6 cut(s)	110, 111, 155, 860, 890, 1248
BmrI	ACTGGG	1 cut(s)	244
BmsI	GCATC	7 cut(s)	296, 321, 568, 1166, 1400, 1564, 1727
BmuI	ACTGGG	1 cut(s)	244
BoxI	GACNNNNGTC	2 cut(s)	1233, 1314
BpmI	CTGGAG	1 cut(s)	881
BpuEI	CTTGAG	1 cut(s)	12
BpuMI	CCSGG	3 cut(s)	110, 111, 155
BsaBI	GATNNNNATC	1 cut(s)	1578
BsaHI	GRCGYC	1 cut(s)	765
BsaI	GGTCTC	1 cut(s)	657
BsaJI	CCNNGG	4 cut(s)	109, 721, 768, 889
BsaXI	ACNNNNNCTCC	4 cut(s)	636, 666, 789, 819
Bsc4I	CCNNNNNNNGG	5 cut(s)	187, 1003, 1092, 1693, 1744
Bse118I	RCCGGY	1 cut(s)	325
Bse1I	ACTGG	2 cut(s)	250, 667
Bse3DI	GCAATG	4 cut(s)	165, 267, 1052, 1207
Bse8I	GATNNNNATC	1 cut(s)	1578
BseBI	CCWGG	3 cut(s)	860, 890, 1248
BseDI	CCNNGG	4 cut(s)	109, 721, 768, 889
BseGI	GGATG	5 cut(s)	499, 1415, 1438, 1555, 1683
BseJI	GATNNNNATC	1 cut(s)	1578
BseLI	CCNNNNNNNGG	5 cut(s)	187, 1003, 1092, 1693, 1744
BseMI	GCAATG	4 cut(s)	165, 267, 1052, 1207
BseMII	CTCAG	2 cut(s)	609, 831
BseNI	ACTGG	2 cut(s)	250, 667
BseRI	GAGGAG	1 cut(s)	974
BseSI	GKGCMC	2 cut(s)	565, 1218
BseXI	GCAGC	5 cut(s)	321, 486, 1456, 1639, 1788
BsgI	GTGCAG	2 cut(s)	1455, 1476
Bsh1236I	CGCG	1 cut(s)	1572
Bsh1285I	CGRYCG	2 cut(s)	349, 880
BshFI	GGCC	8 cut(s)	11, 42, 428, 531, 563, 1171, 1668, 1794
BshNI	GGYRCC	2 cut(s)	1215, 1397
BsiEI	CGRYCG	2 cut(s)	349, 880
BsiHKAI	GWGCWC	2 cut(s)	245, 802
BsiHKCI	CYCGRG	2 cut(s)	109, 565
BsiSI	CCGG	4 cut(s)	110, 155, 188, 326
BslI	CCNNNNNNNGG	5 cut(s)	187, 1003, 1092, 1693, 1744
BsmAI	GTCTC	2 cut(s)	657, 1500
BsnI	GGCC	8 cut(s)	11, 42, 428, 531, 563, 1171, 1668, 1794
Bso31I	GGTCTC	1 cut(s)	657
BsoBI	CYCGRG	2 cut(s)	109, 565
Bsp120I	GGGCCC	1 cut(s)	561
Bsp1286I	GDGCHC	4 cut(s)	245, 565, 802, 1218
Bsp1407I	TGTACA	1 cut(s)	654
Bsp143I	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
Bsp19I	CCATGG	2 cut(s)	721, 768
BspANI	GGCC	8 cut(s)	11, 42, 428, 531, 563, 1171, 1668, 1794
BspCNI	CTCAG	2 cut(s)	610, 830
BspFNI	CGCG	1 cut(s)	1572
BspLI	GGNNCC	8 cut(s)	562, 563, 564, 1084, 1132, 1217, 1399, 1484
BspMAI	CTGCAG	1 cut(s)	504
BspMI	ACCTGC	1 cut(s)	1763
BspPI	GGATC	3 cut(s)	78, 1077, 1090
BspT107I	GGYRCC	2 cut(s)	1215, 1397
BspTNI	GGTCTC	1 cut(s)	657
BsrDI	GCAATG	4 cut(s)	165, 267, 1052, 1207
BsrFI	RCCGGY	1 cut(s)	325
BsrGI	TGTACA	1 cut(s)	654
BsrI	ACTGG	2 cut(s)	250, 667
BssAI	RCCGGY	1 cut(s)	325
BssECI	CCNNGG	4 cut(s)	109, 721, 768, 889
BssMI	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
BssNI	GRCGYC	1 cut(s)	765
BssT1I	CCWWGG	2 cut(s)	721, 768
Bst2UI	CCWGG	3 cut(s)	860, 890, 1248
Bst4CI	ACNGT	7 cut(s)	123, 131, 998, 1203, 1291, 1402, 1789
BstACI	GRCGYC	1 cut(s)	765
BstAPI	GCANNNNNTGC	2 cut(s)	1283, 1404
BstAUI	TGTACA	1 cut(s)	654
BstDEI	CTNAG	2 cut(s)	618, 817
BstDSI	CCRYGG	2 cut(s)	721, 768
BstEII	GGTNACC	1 cut(s)	538
BstF5I	GGATG	5 cut(s)	499, 1415, 1438, 1555, 1683
BstFNI	CGCG	1 cut(s)	1572
BstHHI	GCGC	1 cut(s)	424
BstKTI	GATC	6 cut(s)	86, 216, 349, 880, 1085, 1582
BstMAI	GTCTC	2 cut(s)	657, 1500
BstMBI	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
BstMCI	CGRYCG	2 cut(s)	349, 880
BstMWI	GCNNNNNNNGC	5 cut(s)	430, 434, 971, 1283, 1404
BstNI	CCWGG	3 cut(s)	860, 890, 1248
BstNSI	RCATGY	4 cut(s)	806, 941, 1050, 1157
BstPAI	GACNNNNGTC	2 cut(s)	1233, 1314
BstPI	GGTNACC	1 cut(s)	538
BstSCI	CCNGG	6 cut(s)	108, 109, 153, 858, 888, 1246
BstSFI	CTRYAG	3 cut(s)	500, 906, 1238
BstSLI	GKGCMC	2 cut(s)	565, 1218
BstUI	CGCG	1 cut(s)	1572
BstV1I	GCAGC	5 cut(s)	321, 486, 1456, 1639, 1788
BstX2I	RGATCY	1 cut(s)	1082
BstYI	RGATCY	1 cut(s)	1082
BsuRI	GGCC	8 cut(s)	11, 42, 428, 531, 563, 1171, 1668, 1794
BtgI	CCRYGG	2 cut(s)	721, 768
BtgZI	GCGATG	1 cut(s)	1587
BtsCI	GGATG	5 cut(s)	499, 1415, 1438, 1555, 1683
BtsIMutI	CAGTG	8 cut(s)	119, 136, 257, 674, 1199, 1287, 1312, 1541
BveI	ACCTGC	1 cut(s)	1763
CfoI	GCGC	1 cut(s)	424
Cfr10I	RCCGGY	1 cut(s)	325
Cfr13I	GGNCC	6 cut(s)	9, 41, 561, 562, 1667, 1793
Cfr9I	CCCGGG	1 cut(s)	109
CseI	GACGC	1 cut(s)	773
Csp6I	GTAC	3 cut(s)	647, 655, 732
CviAII	CATG	8 cut(s)	430, 490, 722, 769, 803, 938, 1047, 1154
CviQI	GTAC	3 cut(s)	647, 655, 732
DdeI	CTNAG	2 cut(s)	618, 817
DpnI	GATC	6 cut(s)	85, 215, 348, 879, 1084, 1581
DpnII	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
DraI	TTTAAA	1 cut(s)	58
DraIII	CACNNNGTG	3 cut(s)	394, 1222, 1404
EaeI	YGGCCR	1 cut(s)	426
EciI	GGCGGA	3 cut(s)	235, 1026, 1653
Ecl136II	GAGCTC	1 cut(s)	243
Eco130I	CCWWGG	2 cut(s)	721, 768
Eco24I	GRGCYC	2 cut(s)	245, 565
Eco31I	GGTCTC	1 cut(s)	657
Eco32I	GATATC	1 cut(s)	21
Eco53kI	GAGCTC	1 cut(s)	243
Eco57I	CTGAAG	1 cut(s)	1565
Eco88I	CYCGRG	2 cut(s)	109, 565
Eco91I	GGTNACC	1 cut(s)	538
EcoICRI	GAGCTC	1 cut(s)	243
EcoO109I	RGGNCCY	2 cut(s)	561, 562
EcoO65I	GGTNACC	1 cut(s)	538
EcoRI	GAATTC	2 cut(s)	149, 1834
EcoRII	CCWGG	3 cut(s)	858, 888, 1246
EcoRV	GATATC	1 cut(s)	21
EcoT14I	CCWWGG	2 cut(s)	721, 768
EcoT22I	ATGCAT	1 cut(s)	1159
EcoT38I	GRGCYC	2 cut(s)	245, 565
ErhI	CCWWGG	2 cut(s)	721, 768
FaeI	CATG	8 cut(s)	433, 493, 725, 772, 806, 941, 1050, 1157
FalI	AAGNNNNNCTT	4 cut(s)	203, 235, 1538, 1570
FatI	CATG	8 cut(s)	429, 489, 721, 768, 802, 937, 1046, 1153
FauI	CCCGC	3 cut(s)	404, 1205, 1677
FblI	GTMKAC	1 cut(s)	1237
Fnu4HI	GCNGC	8 cut(s)	307, 310, 500, 809, 1470, 1608, 1653, 1802
FokI	GGATG	5 cut(s)	506, 1422, 1425, 1562, 1670
FriOI	GRGCYC	2 cut(s)	245, 565
Fsp4HI	GCNGC	8 cut(s)	307, 310, 500, 809, 1470, 1608, 1653, 1802
FspBI	CTAG	2 cut(s)	761, 1611
GlaI	GCGC	1 cut(s)	423
GluI	GCNGC	8 cut(s)	307, 310, 500, 809, 1470, 1608, 1653, 1802
GsuI	CTGGAG	1 cut(s)	881
HaeIII	GGCC	8 cut(s)	11, 42, 428, 531, 563, 1171, 1668, 1794
HapII	CCGG	4 cut(s)	110, 155, 188, 326
HgaI	GACGC	1 cut(s)	773
HhaI	GCGC	1 cut(s)	424
Hin1I	GRCGYC	1 cut(s)	765
Hin1II	CATG	8 cut(s)	433, 493, 725, 772, 806, 941, 1050, 1157
Hin6I	GCGC	1 cut(s)	422
HinP1I	GCGC	1 cut(s)	422
HincII	GTYRAC	1 cut(s)	165
HindII	GTYRAC	1 cut(s)	165
HinfI	GANTC	3 cut(s)	353, 385, 1243
HpaII	CCGG	4 cut(s)	110, 155, 188, 326
HphI	GGTGA	4 cut(s)	550, 785, 851, 891
Hpy166II	GTNNAC	4 cut(s)	165, 333, 655, 1238
Hpy188I	TCNGA	7 cut(s)	203, 358, 469, 644, 1087, 1105, 1678
Hpy8I	GTNNAC	4 cut(s)	165, 333, 655, 1238
Hpy99I	CGWCG	3 cut(s)	644, 1793, 1843
HpyAV	CCTTC	5 cut(s)	912, 1540, 1635, 1698, 1812
HpyCH4III	ACNGT	7 cut(s)	123, 131, 998, 1203, 1291, 1402, 1789
HpyCH4IV	ACGT	3 cut(s)	167, 1260, 1381
HpyF10VI	GCNNNNNNNGC	5 cut(s)	430, 434, 971, 1283, 1404
HpyF3I	CTNAG	2 cut(s)	618, 817
HpySE526I	ACGT	3 cut(s)	167, 1260, 1381
Hsp92I	GRCGYC	1 cut(s)	765
Hsp92II	CATG	8 cut(s)	433, 493, 725, 772, 806, 941, 1050, 1157
HspAI	GCGC	1 cut(s)	422
KroI	GCCGGC	1 cut(s)	325
KroNI	GCCGGC	1 cut(s)	327
Kzo9I	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
LmnI	GCTCC	4 cut(s)	248, 797, 934, 1769
Lsp1109I	GCAGC	5 cut(s)	321, 486, 1456, 1639, 1788
LweI	GCATC	7 cut(s)	296, 321, 568, 1166, 1400, 1564, 1727
MaeI	CTAG	2 cut(s)	761, 1611
MaeII	ACGT	3 cut(s)	167, 1260, 1381
MaeIII	GTNAC	5 cut(s)	100, 538, 916, 1307, 1591
MalI	GATC	6 cut(s)	85, 215, 348, 879, 1084, 1581
MboI	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
MboII	GAAGA	2 cut(s)	69, 223
MfeI	CAATTG	1 cut(s)	1077
MflI	RGATCY	1 cut(s)	1082
MhlI	GDGCHC	4 cut(s)	245, 565, 802, 1218
MlsI	TGGCCA	1 cut(s)	428
MluNI	TGGCCA	1 cut(s)	428
MmeI	TCCRAC	5 cut(s)	111, 362, 622, 775, 1466
Mox20I	TGGCCA	1 cut(s)	428
Mph1103I	ATGCAT	1 cut(s)	1159
MreI	CGCCGGCG	1 cut(s)	325
MroNI	GCCGGC	1 cut(s)	325
MscI	TGGCCA	1 cut(s)	428
MseI	TTAA	7 cut(s)	36, 57, 969, 1178, 1266, 1355, 1535
MslI	CAYNNNNRTG	3 cut(s)	143, 1419, 1598
Msp20I	TGGCCA	1 cut(s)	428
MspA1I	CMGCKG	1 cut(s)	306
MspI	CCGG	4 cut(s)	110, 155, 188, 326
MspR9I	CCNGG	6 cut(s)	110, 111, 155, 860, 890, 1248
MunI	CAATTG	1 cut(s)	1077
MvaI	CCWGG	3 cut(s)	860, 890, 1248
MvnI	CGCG	1 cut(s)	1572
MwoI	GCNNNNNNNGC	5 cut(s)	430, 434, 971, 1283, 1404
NaeI	GCCGGC	1 cut(s)	327
NciI	CCSGG	3 cut(s)	110, 111, 155
NcoI	CCATGG	2 cut(s)	721, 768
NdeII	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
NgoMIV	GCCGGC	1 cut(s)	325
NlaIII	CATG	8 cut(s)	433, 493, 725, 772, 806, 941, 1050, 1157
NlaIV	GGNNCC	8 cut(s)	562, 563, 564, 1084, 1132, 1217, 1399, 1484
NmuCI	GTSAC	4 cut(s)	100, 538, 1307, 1591
NsiI	ATGCAT	1 cut(s)	1159
NspI	RCATGY	4 cut(s)	806, 941, 1050, 1157
OliI	CACNNNNGTG	1 cut(s)	1598
PaeI	GCATGC	1 cut(s)	1157
PciI	ACATGT	1 cut(s)	802
PcsI	WCGNNNNNNNCGW	1 cut(s)	648
PdiI	GCCGGC	1 cut(s)	327
PfeI	GAWTC	3 cut(s)	353, 385, 1243
PfoI	TCCNGGA	2 cut(s)	858, 1246
PkrI	GCNGC	8 cut(s)	308, 311, 501, 810, 1471, 1609, 1654, 1803
Ple19I	CGATCG	2 cut(s)	349, 880
PscI	ACATGT	1 cut(s)	802
PshAI	GACNNNNGTC	2 cut(s)	1233, 1314
Psp124BI	GAGCTC	1 cut(s)	245
Psp6I	CCWGG	3 cut(s)	858, 888, 1246
PspEI	GGTNACC	1 cut(s)	538
PspGI	CCWGG	3 cut(s)	858, 888, 1246
PspN4I	GGNNCC	8 cut(s)	562, 563, 564, 1084, 1132, 1217, 1399, 1484
PspOMI	GGGCCC	1 cut(s)	561
PspPI	GGNCC	6 cut(s)	9, 41, 561, 562, 1667, 1793
PstI	CTGCAG	1 cut(s)	504
PsuI	RGATCY	1 cut(s)	1082
PvuI	CGATCG	2 cut(s)	349, 880
RsaI	GTAC	3 cut(s)	648, 656, 733
RsaNI	GTAC	3 cut(s)	647, 655, 732
RseI	CAYNNNNRTG	3 cut(s)	143, 1419, 1598
SacI	GAGCTC	1 cut(s)	245
SaqAI	TTAA	7 cut(s)	36, 57, 969, 1178, 1266, 1355, 1535
SatI	GCNGC	8 cut(s)	307, 310, 500, 809, 1470, 1608, 1653, 1802
Sau3AI	GATC	6 cut(s)	83, 213, 346, 877, 1082, 1579
Sau96I	GGNCC	6 cut(s)	9, 41, 561, 562, 1667, 1793
ScrFI	CCNGG	6 cut(s)	110, 111, 155, 860, 890, 1248
SduI	GDGCHC	4 cut(s)	245, 565, 802, 1218
SfaNI	GCATC	7 cut(s)	296, 321, 568, 1166, 1400, 1564, 1727
SfcI	CTRYAG	3 cut(s)	500, 906, 1238
SgrAI	CRCCGGYG	1 cut(s)	325
SmaI	CCCGGG	1 cut(s)	111
SmiMI	CAYNNNNRTG	3 cut(s)	143, 1419, 1598
SmlI	CTYRAG	1 cut(s)	27
SmoI	CTYRAG	1 cut(s)	27
SphI	GCATGC	1 cut(s)	1157
SspMI	CTAG	2 cut(s)	761, 1611
SstI	GAGCTC	1 cut(s)	245
StyD4I	CCNGG	6 cut(s)	108, 109, 153, 858, 888, 1246
StyI	CCWWGG	2 cut(s)	721, 768
TaaI	ACNGT	7 cut(s)	123, 131, 998, 1203, 1291, 1402, 1789
TaiI	ACGT	3 cut(s)	170, 1263, 1384
TaqI	TCGA	4 cut(s)	216, 290, 349, 1626
TatI	WGTACW	2 cut(s)	654, 731
TauI	GCSGC	3 cut(s)	309, 811, 1610
TfiI	GAWTC	3 cut(s)	353, 385, 1243
Tru1I	TTAA	7 cut(s)	36, 57, 969, 1178, 1266, 1355, 1535
Tru9I	TTAA	7 cut(s)	36, 57, 969, 1178, 1266, 1355, 1535
TscAI	CASTG	8 cut(s)	126, 136, 257, 674, 1206, 1294, 1312, 1548
TseFI	GTSAC	4 cut(s)	100, 538, 1307, 1591
TseI	GCWGC	5 cut(s)	309, 499, 1469, 1652, 1801
Tsp45I	GTSAC	4 cut(s)	100, 538, 1307, 1591
TspDTI	ATGAA	9 cut(s)	17, 478, 885, 1041, 1161, 1244, 1354, 1824, 1835
TspGWI	ACGGA	4 cut(s)	55, 268, 350, 628
TspMI	CCCGGG	1 cut(s)	109
TspRI	CASTG	8 cut(s)	126, 136, 257, 674, 1206, 1294, 1312, 1548
XapI	RAATTY	5 cut(s)	149, 922, 986, 1174, 1834
XbaI	TCTAGA	1 cut(s)	760
XceI	RCATGY	4 cut(s)	806, 941, 1050, 1157
XmaI	CCCGGG	1 cut(s)	109
XmiI	GTMKAC	1 cut(s)	1237
XspI	CTAG	2 cut(s)	761, 1611
Zsp2I	ATGCAT	1 cut(s)	1159

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.