Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009723

Physical Location & Seq

Genomic Coordinates Reverse (-)

34333396 .. 34334928

Gene Structure 1533 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1278800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 546 bp

ATGCTTGCCCTAAAACAGGGTTATGAGGTTAGCGGCTCGGATATTGCTTGGAGTAGCTTCGTGGACGGGCTGCAGGAAGCAGGGGCATTGTTGCACACAGGCCATTCGGTGGCTAATATACACGGGAGTGGCGCCTCGAGATTACCTGAAGCTGCTTTTGTATGCAATGTCCGTCGGAGTTCCTGTATTGTGTGCAACTGTATATATGACGATTATGCTCATCATCCATCAGAAGTTAGTGCAGTTATTCAAGCTGCACGCCCGAGGTTCCCTGTCAAGTCTCCTATGGTCGTATTCCAGCCTCATATGTACAGTCGTTTAGCAGCAATGAAGGATGACTTCGCTAATGCCCTCCGTGGTGCAGATCAAGCCGTAGTCACAGAGGTATGTTGCCTGGACACACGGTTTTATGCTGCTAGAGAAATAGATGTCCAGAATGTTGGTGGGAGGGAGTTAGCTGCTACTGTAATTGGCCCGCCATCTGAATGCATCCCTTCTTTGGTAATACCAGTGTTTTCAAATTGTTTAGAACACATTTGTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

182

Amino Acids

19.54

Weight (kDa)

5.74

Isoelectric Point (pI)

51.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase	PF01225	3 - 92	1e-05	Mur ligase family, catalytic domain
Mur_ligase_C	PF02875	68 - 131	1.1e-08	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009723)

Species	Orthologous Gene IDs
malus_domestica	MD01G1208200.v1.1 MD07G1278800.v1.1
prunus_persica	Prupe.2G300700_v2.0.a1
pyrus_communis	pycom01g21880
rosa_chinensis	RchiOBHm_Chr1g0378261
rosa_laevigata	RLG00000026454 RLG00000026455
rosa_multiflora	Rmu_sc0006315.1_g000004 Rmu_sc0006315.1_g000010 Rmu_sc0013545.1_g000004
rosa_roxburghii	Rroxscaffold_4G00280540 Rroxscaffold_4G00280550 Rroxscaffold_4G00280600
rosa_rugosa	Rorug01G0407600 Rorug01G0407800
rosa_samantha	Rh1AG421700 Rh1AG422200 Rh1BG386900 Rh1CG400400 Rh1DG418000
rosa_wichuraiana	Rw0G013300 Rw1G037520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	131
AccB7I	CCANNNNNTGG	1 cut(s)	109
AciI	CCGC	2 cut(s)	33, 476
AcuI	CTGAAG	1 cut(s)	168
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	1 cut(s)	311
AfiI	CCNNNNNNNGG	3 cut(s)	16, 109, 499
AgsI	TTSAA	2 cut(s)	251, 519
AjnI	CCWGG	1 cut(s)	393
AleI	CACNNNNGTG	1 cut(s)	126
AluBI	AGCT	4 cut(s)	57, 152, 254, 458
AluI	AGCT	4 cut(s)	57, 152, 254, 458
Alw26I	GTCTC	1 cut(s)	285
Ama87I	CYCGRG	2 cut(s)	136, 262
AoxI	GGCC	2 cut(s)	100, 472
ApeKI	GCWGC	6 cut(s)	70, 152, 254, 323, 413, 458
AspLEI	GCGC	1 cut(s)	134
AspS9I	GGNCC	1 cut(s)	473
AvaI	CYCGRG	2 cut(s)	136, 262
BanI	GGYRCC	1 cut(s)	131
BbvI	GCAGC	6 cut(s)	57, 139, 241, 335, 400, 445
BccI	CCATC	2 cut(s)	235, 487
BceAI	ACGGC	1 cut(s)	356
BciT130I	CCWGG	1 cut(s)	395
BcoDI	GTCTC	1 cut(s)	285
BfaI	CTAG	1 cut(s)	417
BfmI	CTRYAG	1 cut(s)	71
BfoI	RGCGCY	1 cut(s)	135
BisI	GCNGC	7 cut(s)	34, 71, 153, 255, 324, 414, 459
BlsI	GCNGC	7 cut(s)	35, 72, 154, 256, 325, 415, 460
Bme1390I	CCNGG	1 cut(s)	395
BmeT110I	CYCGRG	2 cut(s)	136, 262
BmgT120I	GGNCC	1 cut(s)	473
BmiI	GGNNCC	2 cut(s)	133, 269
BmrFI	CCNGG	1 cut(s)	395
BmsI	GCATC	1 cut(s)	498
BsaHI	GRCGYC	1 cut(s)	132
BsaJI	CCNNGG	2 cut(s)	263, 355
BsaXI	ACNNNNNCTCC	2 cut(s)	169, 199
Bsc4I	CCNNNNNNNGG	3 cut(s)	16, 109, 499
Bse1I	ACTGG	1 cut(s)	509
Bse3DI	GCAATG	2 cut(s)	172, 333
BseBI	CCWGG	1 cut(s)	395
BseDI	CCNNGG	2 cut(s)	263, 355
BseGI	GGATG	3 cut(s)	223, 340, 489
BseLI	CCNNNNNNNGG	3 cut(s)	16, 109, 499
BseMI	GCAATG	2 cut(s)	172, 333
BseNI	ACTGG	1 cut(s)	509
BseXI	GCAGC	6 cut(s)	57, 139, 241, 335, 400, 445
BsgI	GTGCAG	3 cut(s)	240, 261, 381
BshFI	GGCC	2 cut(s)	102, 474
BshNI	GGYRCC	1 cut(s)	131
BsiHKCI	CYCGRG	2 cut(s)	136, 262
BslI	CCNNNNNNNGG	3 cut(s)	16, 109, 499
BsmAI	GTCTC	1 cut(s)	285
BsmI	GAATGC	1 cut(s)	491
BsnI	GGCC	2 cut(s)	102, 474
BsoBI	CYCGRG	2 cut(s)	136, 262
Bsp1407I	TGTACA	1 cut(s)	309
Bsp143I	GATC	1 cut(s)	364
BspACI	CCGC	2 cut(s)	33, 476
BspANI	GGCC	2 cut(s)	102, 474
BspLI	GGNNCC	2 cut(s)	133, 269
BspMAI	CTGCAG	1 cut(s)	75
BspT107I	GGYRCC	1 cut(s)	131
BsrDI	GCAATG	2 cut(s)	172, 333
BsrGI	TGTACA	1 cut(s)	309
BsrI	ACTGG	1 cut(s)	509
BssECI	CCNNGG	2 cut(s)	263, 355
BssMI	GATC	1 cut(s)	364
BssNI	GRCGYC	1 cut(s)	132
Bst2UI	CCWGG	1 cut(s)	395
Bst4CI	ACNGT	4 cut(s)	200, 314, 405, 466
BstACI	GRCGYC	1 cut(s)	132
BstAUI	TGTACA	1 cut(s)	309
BstC8I	GCNNGC	3 cut(s)	6, 259, 476
BstDSI	CCRYGG	1 cut(s)	355
BstENI	CCTNNNNNAGG	1 cut(s)	14
BstF5I	GGATG	3 cut(s)	223, 340, 489
BstH2I	RGCGCY	1 cut(s)	135
BstHHI	GCGC	1 cut(s)	134
BstKTI	GATC	1 cut(s)	367
BstMAI	GTCTC	1 cut(s)	285
BstMBI	GATC	1 cut(s)	364
BstMWI	GCNNNNNNNGC	1 cut(s)	368
BstNI	CCWGG	1 cut(s)	395
BstSCI	CCNGG	1 cut(s)	393
BstSFI	CTRYAG	1 cut(s)	71
BstV1I	GCAGC	6 cut(s)	57, 139, 241, 335, 400, 445
BstXI	CCANNNNNNTGG	1 cut(s)	440
BsuRI	GGCC	2 cut(s)	102, 474
BtgI	CCRYGG	1 cut(s)	355
BtsCI	GGATG	3 cut(s)	223, 340, 489
BtsIMutI	CAGTG	1 cut(s)	516
Cac8I	GCNNGC	3 cut(s)	6, 259, 476
CfoI	GCGC	1 cut(s)	134
Cfr13I	GGNCC	1 cut(s)	473
Csp6I	GTAC	1 cut(s)	310
CviQI	GTAC	1 cut(s)	310
DinI	GGCGCC	1 cut(s)	133
DpnI	GATC	1 cut(s)	366
DpnII	GATC	1 cut(s)	364
Eco57I	CTGAAG	1 cut(s)	168
Eco88I	CYCGRG	2 cut(s)	136, 262
EcoNI	CCTNNNNNAGG	1 cut(s)	14
EcoRII	CCWGG	1 cut(s)	393
EcoT22I	ATGCAT	1 cut(s)	491
EgeI	GGCGCC	1 cut(s)	133
EheI	GGCGCC	1 cut(s)	133
FalI	AAGNNNNNCTT	2 cut(s)	323, 355
FauI	CCCGC	1 cut(s)	483
FauNDI	CATATG	1 cut(s)	306
Fnu4HI	GCNGC	7 cut(s)	34, 71, 153, 255, 324, 414, 459
FokI	GGATG	3 cut(s)	210, 347, 476
Fsp4HI	GCNGC	7 cut(s)	34, 71, 153, 255, 324, 414, 459
FspBI	CTAG	1 cut(s)	417
GlaI	GCGC	1 cut(s)	133
GluI	GCNGC	7 cut(s)	34, 71, 153, 255, 324, 414, 459
HaeII	RGCGCY	1 cut(s)	135
HaeIII	GGCC	2 cut(s)	102, 474
HhaI	GCGC	1 cut(s)	134
Hin1I	GRCGYC	1 cut(s)	132
Hin6I	GCGC	1 cut(s)	132
HinP1I	GCGC	1 cut(s)	132
Hpy166II	GTNNAC	1 cut(s)	64
Hpy188I	TCNGA	5 cut(s)	40, 177, 232, 484, 545
Hpy188III	TCNNGA	2 cut(s)	138, 433
Hpy8I	GTNNAC	1 cut(s)	64
Hpy99I	CGWCG	1 cut(s)	177
HpyAV	CCTTC	2 cut(s)	325, 504
HpyCH4III	ACNGT	4 cut(s)	200, 314, 405, 466
HpyCH4V	TGCA	8 cut(s)	73, 94, 165, 195, 242, 257, 362, 489
HpyF10VI	GCNNNNNNNGC	1 cut(s)	368
Hsp92I	GRCGYC	1 cut(s)	132
HspAI	GCGC	1 cut(s)	132
KasI	GGCGCC	1 cut(s)	131
Kzo9I	GATC	1 cut(s)	364
Lsp1109I	GCAGC	6 cut(s)	57, 139, 241, 335, 400, 445
LweI	GCATC	1 cut(s)	498
MaeI	CTAG	1 cut(s)	417
MaeIII	GTNAC	1 cut(s)	376
MalI	GATC	1 cut(s)	366
MboI	GATC	1 cut(s)	364
MluCI	AATT	2 cut(s)	468, 520
Mly113I	GGCGCC	1 cut(s)	132
MmeI	TCCRAC	1 cut(s)	155
MnlI	CCTC	7 cut(s)	19, 145, 258, 312, 362, 376, 441
Mph1103I	ATGCAT	1 cut(s)	491
MslI	CAYNNNNRTG	2 cut(s)	126, 484
MspR9I	CCNGG	1 cut(s)	395
Mva1269I	GAATGC	1 cut(s)	491
MvaI	CCWGG	1 cut(s)	395
MwoI	GCNNNNNNNGC	1 cut(s)	368
NarI	GGCGCC	1 cut(s)	132
NdeI	CATATG	1 cut(s)	306
NdeII	GATC	1 cut(s)	364
NlaIV	GGNNCC	2 cut(s)	133, 269
NmuCI	GTSAC	1 cut(s)	376
NsiI	ATGCAT	1 cut(s)	491
OliI	CACNNNNGTG	1 cut(s)	126
PaeR7I	CTCGAG	1 cut(s)	136
PctI	GAATGC	1 cut(s)	491
PflMI	CCANNNNNTGG	1 cut(s)	109
PkrI	GCNGC	7 cut(s)	35, 72, 154, 256, 325, 415, 460
PluTI	GGCGCC	1 cut(s)	135
Psp6I	CCWGG	1 cut(s)	393
PspGI	CCWGG	1 cut(s)	393
PspN4I	GGNNCC	2 cut(s)	133, 269
PspPI	GGNCC	1 cut(s)	473
PstI	CTGCAG	1 cut(s)	75
RsaI	GTAC	1 cut(s)	311
RsaNI	GTAC	1 cut(s)	310
RseI	CAYNNNNRTG	2 cut(s)	126, 484
SatI	GCNGC	7 cut(s)	34, 71, 153, 255, 324, 414, 459
Sau3AI	GATC	1 cut(s)	364
Sau96I	GGNCC	1 cut(s)	473
ScrFI	CCNGG	1 cut(s)	395
SetI	ASST	8 cut(s)	30, 59, 148, 154, 256, 269, 387, 460
SfaNI	GCATC	1 cut(s)	498
SfcI	CTRYAG	1 cut(s)	71
SfoI	GGCGCC	1 cut(s)	133
Sfr274I	CTCGAG	1 cut(s)	136
SlaI	CTCGAG	1 cut(s)	136
SmiMI	CAYNNNNRTG	2 cut(s)	126, 484
SmlI	CTYRAG	1 cut(s)	136
SmoI	CTYRAG	1 cut(s)	136
Sse9I	AATT	2 cut(s)	468, 520
SsiI	CCGC	2 cut(s)	33, 476
SspDI	GGCGCC	1 cut(s)	131
SspMI	CTAG	1 cut(s)	417
StyD4I	CCNGG	1 cut(s)	393
TaaI	ACNGT	4 cut(s)	200, 314, 405, 466
TaqI	TCGA	1 cut(s)	137
TasI	AATT	2 cut(s)	468, 520
TatI	WGTACW	1 cut(s)	309
TauI	GCSGC	1 cut(s)	36
TscAI	CASTG	1 cut(s)	516
TseFI	GTSAC	1 cut(s)	376
TseI	GCWGC	6 cut(s)	70, 152, 254, 323, 413, 458
Tsp45I	GTSAC	1 cut(s)	376
TspDTI	ATGAA	1 cut(s)	344
TspGWI	ACGGA	2 cut(s)	161, 344
TspRI	CASTG	1 cut(s)	516
Van91I	CCANNNNNTGG	1 cut(s)	109
XagI	CCTNNNNNAGG	1 cut(s)	14
XcmI	CCANNNNNNNNNTGG	1 cut(s)	440
XhoI	CTCGAG	1 cut(s)	136
XspI	CTAG	1 cut(s)	417
Zsp2I	ATGCAT	1 cut(s)	491

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.