Rh1AG422200

Mur ligase family, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0009723
Physical Location & Seq
Forward (+)
65197214 .. 65204325
7112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG422200.1

Sequence Viewer

Length: 1497 bp
ATGATATTCCCGGCAATTTGTCCTTCCAAAACCCTCCATTTTCCCGCCAATTTTACACCCAAGCCAAAGCTTCACTTCTCGGAAAGATCTCCGGCCACTCCAAACCCAACCACACTGGAATTTCCTGGACCGAAAACAGTAGTGGCTTCTCAATCTCGACGAGTTGCAACGACGAGAGAGAGCCAGAATTGGAGAATTCTCGAAACGATAAGAGTTGGATTCACTTCGTCGGTATCGGAGGCTGTGGCTTGTCCGCGCTGGCCATGCGTGCACTCAAACCGGTACAAGCGAGACCATTGGTTAGGACAGCTGACACAGGGCTACAATCTTATTGCTGTAAGCAGTAGCCATGGAAAGAGTACAACTGCAAGTATGCTTGCTTATGTTCTAGACGCCATGGGTGATAGTCTTACAGCAGTAGTTGGAGCTCACATGCCACAGTTCTCGGGTGGAAACATTATCTTGGGAGATGGTCAAAATTTTGTGCTAGAGGCTGATGAATATGATGGTTGCTTCCTGGGACTATCACCTTATATAGCTGTTGTAACAAATCTGGATTGGGAGCATGTTGATATTTTCGAAAACGAGGAAGCAGTTAAAGCTACTTTCCATAAATTCCTGAATCTAATCAGGGTGGGTGGACATCTCATTTTATGTGGGGATAGTCAGGGTGCCTATTCCTTGCTGACTGATGTAAAGCAAGCAATTGGATCAGATACTTTGAGCGGTCTAATGCATTCTCCGCTAGAAAAATGTTGCATTGGCTACATCATAACAACTTATGGAACTACAAGTTCTAATGAATGGAGTGCATCATCAATTAGGCCAAATTTAAAGGGTGGTAGTGATTATACACTGTGTCATTGGGGGAGCTCAGTGGCAGACATCAGTCTTCAGGTTCCAGGAGTTCATAATGTCCTAAATTCACTAGCAGTTATTGCCACTGTGATAGCATTATACAATAACCAACGTGAAATTAACAATACAATAAATGTTCAAGCAGTTATTCAAGCTGCCCGCCAGAAGTTCCCTATCAAGTCTCTTTTAGTGGTATTTCAGCCTCATACTTACAGTCGTTTAGCAGCACTGAAGGATGATTTTGCCAGTGCCCTCTGTGGTGCAGATCAAGTTGTAGTTACAGAGGTTTATGCTGCTAGAGAAATAGATGTTGGGAACGTTGGTGGAAGGGAGTTAGCTGCTTCTGTAATCGGCCCGCCATGTGAATACATCCCTTCTCTGGGTGATGTGGTAGAGAGGTTGGTATATCAAATATGCAAGAACCCTCATCATGATACCATTGTCTTAACACTTGGAGCAGGTGATATAACAACAGTGGGGCCAAAGCTGCTCTATGAATTGCAAATAAGTTACAATTCATTTGGTTGCAAAGTGCATTTAAGATTAACAGTTATAAGCATAGACAACCAAATTCATCAAATGGTACTGCAAATATCAGGCACGAACTTTACAATCCATAGAGTTCAAGAGACCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

498

Amino Acids

54.34

Weight (kDa)

6.4

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_M PF08245 116 - 224 1.2e-09 Mur ligase middle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1412
AarI CACCTGC 1 cut(s) 1307
Acc36I ACCTGC 1 cut(s) 1307
AccB1I GGYRCC 1 cut(s) 671
AccBSI CCGCTC 1 cut(s) 726
AccII CGCG 1 cut(s) 256
AciI CCGC 6 cut(s) 45, 254, 726, 743, 1018, 1214
AclI AACGTT 1 cut(s) 1176
AclWI GGATC 1 cut(s) 718
AcoI YGGCCR 2 cut(s) 93, 260
AcsI RAATTY 7 cut(s) 119, 195, 478, 614, 829, 922, 1428
AcuI CTGAAG 2 cut(s) 878, 1109
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 3 cut(s) 284, 361, 1443
AfiI CCNNNNNNNGG 2 cut(s) 1237, 1238
AgeI ACCGGT 1 cut(s) 279
AgsI TTSAA 3 cut(s) 998, 1010, 1484
AjnI CCWGG 3 cut(s) 124, 516, 901
AjuI GAANNNNNNNTTGG 2 cut(s) 1152, 1184
AluBI AGCT 9 cut(s) 70, 310, 428, 539, 602, 873, 1013, 1196, 1345
AluI AGCT 9 cut(s) 70, 310, 428, 539, 602, 873, 1013, 1196, 1345
Alw21I GWGCWC 3 cut(s) 273, 430, 875
Alw26I GTCTC 3 cut(s) 285, 1044, 1481
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 5 cut(s) 93, 260, 824, 1210, 1337
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 5 cut(s) 1013, 1082, 1151, 1196, 1345
ApoI RAATTY 7 cut(s) 119, 195, 478, 614, 829, 922, 1428
AsiGI ACCGGT 1 cut(s) 279
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 3 cut(s) 128, 1211, 1337
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 4 cut(s) 413, 519, 1253, 1331
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 445
AvaII GGWCC 1 cut(s) 128
BaeGI GKGCMC 2 cut(s) 273, 1111
BaeI ACNNNNGTAYC 2 cut(s) 1284, 1317
BalI TGGCCA 1 cut(s) 262
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 2 cut(s) 430, 875
BbsI GAAGAC 1 cut(s) 884
Bbv12I GWGCWC 3 cut(s) 273, 430, 875
BbvI GCAGC 5 cut(s) 1000, 1094, 1138, 1183, 1332
BccI CCATC 2 cut(s) 464, 500
BciT130I CCWGG 3 cut(s) 126, 518, 903
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 3 cut(s) 285, 1044, 1481
BfaI CTAG 5 cut(s) 389, 488, 746, 929, 1155
BfuAI ACCTGC 1 cut(s) 1307
BglII AGATCT 1 cut(s) 86
BisI GCNGC 5 cut(s) 1014, 1083, 1152, 1197, 1346
BlsI GCNGC 5 cut(s) 1015, 1084, 1153, 1198, 1347
Bme1390I CCNGG 4 cut(s) 11, 126, 518, 903
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 445
BmgT120I GGNCC 3 cut(s) 128, 1211, 1337
BmiI GGNNCC 3 cut(s) 673, 900, 1338
BmrFI CCNGG 4 cut(s) 11, 126, 518, 903
BmsI GCATC 1 cut(s) 821
BoxI GACNNNNGTC 1 cut(s) 888
BpiI GAAGAC 1 cut(s) 884
Bpu14I TTCGAA 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 2 cut(s) 285, 1481
BsaJI CCNNGG 3 cut(s) 349, 396, 517
BsaWI WCCGGW 1 cut(s) 279
Bsc4I CCNNNNNNNGG 2 cut(s) 1237, 1238
Bse118I RCCGGY 1 cut(s) 279
Bse1I ACTGG 2 cut(s) 120, 1104
BseBI CCWGG 3 cut(s) 126, 518, 903
BseDI CCNNGG 3 cut(s) 349, 396, 517
BseGI GGATG 2 cut(s) 1099, 1227
BseLI CCNNNNNNNGG 2 cut(s) 1237, 1238
BseMII CTCAG 1 cut(s) 888
BseNI ACTGG 2 cut(s) 120, 1104
BseSI GKGCMC 2 cut(s) 273, 1111
BseXI GCAGC 5 cut(s) 1000, 1094, 1138, 1183, 1332
BsgI GTGCAG 1 cut(s) 1140
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 5 cut(s) 95, 262, 826, 1212, 1339
BshNI GGYRCC 1 cut(s) 671
BshTI ACCGGT 1 cut(s) 279
BsiHKAI GWGCWC 3 cut(s) 273, 430, 875
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 3 cut(s) 11, 92, 280
BslFI GGGAC 1 cut(s) 534
BslI CCNNNNNNNGG 2 cut(s) 1237, 1238
BsmAI GTCTC 3 cut(s) 285, 1044, 1481
BsmFI GGGAC 1 cut(s) 534
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 5 cut(s) 95, 262, 826, 1212, 1339
Bso31I GGTCTC 2 cut(s) 285, 1481
BsoBI CYCGRG 1 cut(s) 445
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 4 cut(s) 273, 430, 875, 1111
Bsp143I GATC 3 cut(s) 86, 710, 1123
Bsp19I CCATGG 2 cut(s) 349, 396
BspACI CCGC 6 cut(s) 45, 254, 726, 743, 1018, 1214
BspANI GGCC 5 cut(s) 95, 262, 826, 1212, 1339
BspCNI CTCAG 1 cut(s) 887
BspFNI CGCG 1 cut(s) 256
BspHI TCATGA 1 cut(s) 1288
BspLI GGNNCC 3 cut(s) 673, 900, 1338
BspMI ACCTGC 1 cut(s) 1307
BspPI GGATC 1 cut(s) 718
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 671
BspTNI GGTCTC 2 cut(s) 285, 1481
BsrBI CCGCTC 1 cut(s) 726
BsrFI RCCGGY 1 cut(s) 279
BsrI ACTGG 2 cut(s) 120, 1104
BssAI RCCGGY 1 cut(s) 279
BssECI CCNNGG 3 cut(s) 349, 396, 517
BssMI GATC 3 cut(s) 86, 710, 1123
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 2 cut(s) 349, 396
Bst2UI CCWGG 3 cut(s) 126, 518, 903
Bst4CI ACNGT 7 cut(s) 139, 441, 858, 946, 1073, 1333, 1408
BstACI GRCGYC 1 cut(s) 393
BstAPI GCANNNNNTGC 1 cut(s) 938
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 6 cut(s) 260, 269, 378, 702, 1018, 1214
BstDEI CTNAG 1 cut(s) 874
BstDSI CCRYGG 2 cut(s) 349, 396
BstF5I GGATG 2 cut(s) 1099, 1227
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 3 cut(s) 89, 713, 1126
BstMAI GTCTC 3 cut(s) 285, 1044, 1481
BstMBI GATC 3 cut(s) 86, 710, 1123
BstMWI GCNNNNNNNGC 6 cut(s) 264, 268, 599, 742, 938, 1345
BstNI CCWGG 3 cut(s) 126, 518, 903
BstNSI RCATGY 2 cut(s) 436, 569
BstPAI GACNNNNGTC 1 cut(s) 888
BstSCI CCNGG 4 cut(s) 9, 124, 516, 901
BstSLI GKGCMC 2 cut(s) 273, 1111
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 5 cut(s) 1000, 1094, 1138, 1183, 1332
BstV2I GAAGAC 1 cut(s) 884
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
BsuRI GGCC 5 cut(s) 95, 262, 826, 1212, 1339
BtgI CCRYGG 2 cut(s) 349, 396
BtsCI GGATG 2 cut(s) 1099, 1227
BtsIMutI CAGTG 7 cut(s) 113, 854, 882, 942, 1085, 1111, 1338
BveI ACCTGC 1 cut(s) 1307
Cac8I GCNNGC 6 cut(s) 260, 269, 378, 702, 1018, 1214
CciI TCATGA 1 cut(s) 1288
CfoI GCGC 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 279
Cfr13I GGNCC 3 cut(s) 128, 1211, 1337
CseI GACGC 1 cut(s) 401
Csp6I GTAC 3 cut(s) 283, 360, 1442
CspAI ACCGGT 1 cut(s) 279
CviAII CATG 7 cut(s) 264, 350, 397, 433, 566, 1218, 1289
CviQI GTAC 3 cut(s) 283, 360, 1442
DdeI CTNAG 1 cut(s) 874
DpnI GATC 3 cut(s) 88, 712, 1125
DpnII GATC 3 cut(s) 86, 710, 1123
DraI TTTAAA 1 cut(s) 834
EaeI YGGCCR 2 cut(s) 93, 260
Ecl136II GAGCTC 2 cut(s) 428, 873
Eco130I CCWWGG 2 cut(s) 349, 396
Eco24I GRGCYC 2 cut(s) 430, 875
Eco31I GGTCTC 2 cut(s) 285, 1481
Eco47I GGWCC 1 cut(s) 128
Eco53kI GAGCTC 2 cut(s) 428, 873
Eco57I CTGAAG 2 cut(s) 878, 1109
Eco88I CYCGRG 1 cut(s) 445
EcoICRI GAGCTC 2 cut(s) 428, 873
EcoRI GAATTC 1 cut(s) 195
EcoRII CCWGG 3 cut(s) 124, 516, 901
EcoT14I CCWWGG 2 cut(s) 349, 396
EcoT22I ATGCAT 1 cut(s) 738
EcoT38I GRGCYC 2 cut(s) 430, 875
ErhI CCWWGG 2 cut(s) 349, 396
FaeI CATG 7 cut(s) 267, 353, 400, 436, 569, 1221, 1292
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 1 cut(s) 534
FatI CATG 7 cut(s) 263, 349, 396, 432, 565, 1217, 1288
FauI CCCGC 3 cut(s) 52, 1025, 1221
Fnu4HI GCNGC 5 cut(s) 1014, 1083, 1152, 1197, 1346
FokI GGATG 2 cut(s) 1106, 1214
FriOI GRGCYC 2 cut(s) 430, 875
Fsp4HI GCNGC 5 cut(s) 1014, 1083, 1152, 1197, 1346
FspBI CTAG 5 cut(s) 389, 488, 746, 929, 1155
GlaI GCGC 1 cut(s) 257
GluI GCNGC 5 cut(s) 1014, 1083, 1152, 1197, 1346
HaeIII GGCC 5 cut(s) 95, 262, 826, 1212, 1339
HapII CCGG 3 cut(s) 11, 92, 280
HgaI GACGC 1 cut(s) 401
HhaI GCGC 1 cut(s) 258
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 7 cut(s) 267, 353, 400, 436, 569, 1221, 1292
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 2 cut(s) 219, 622
HpaII CCGG 3 cut(s) 11, 92, 280
HphI GGTGA 4 cut(s) 413, 519, 1253, 1331
Hpy166II GTNNAC 2 cut(s) 271, 641
Hpy188I TCNGA 3 cut(s) 82, 238, 715
Hpy188III TCNNGA 7 cut(s) 156, 200, 389, 554, 619, 1289, 1484
Hpy8I GTNNAC 2 cut(s) 271, 641
Hpy99I CGWCG 3 cut(s) 162, 175, 232
HpyAV CCTTC 4 cut(s) 33, 1084, 1179, 1242
HpyCH4III ACNGT 7 cut(s) 139, 441, 858, 946, 1073, 1333, 1408
HpyCH4IV ACGT 2 cut(s) 970, 1176
HpyF10VI GCNNNNNNNGC 6 cut(s) 264, 268, 599, 742, 938, 1345
HpyF3I CTNAG 1 cut(s) 874
HpySE526I ACGT 2 cut(s) 970, 1176
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 7 cut(s) 267, 353, 400, 436, 569, 1221, 1292
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 3 cut(s) 86, 710, 1123
LmnI GCTCC 4 cut(s) 425, 562, 870, 1313
Lsp1109I GCAGC 5 cut(s) 1000, 1094, 1138, 1183, 1332
LweI GCATC 1 cut(s) 821
MaeI CTAG 5 cut(s) 389, 488, 746, 929, 1155
MaeII ACGT 2 cut(s) 970, 1176
MaeIII GTNAC 3 cut(s) 544, 1135, 1367
MalI GATC 3 cut(s) 88, 712, 1125
MbiI CCGCTC 1 cut(s) 726
MboI GATC 3 cut(s) 86, 710, 1123
MboII GAAGA 1 cut(s) 884
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 4 cut(s) 273, 430, 875, 1111
MlsI TGGCCA 1 cut(s) 262
MluNI TGGCCA 1 cut(s) 262
MmeI TCCRAC 2 cut(s) 196, 403
MnlI CCTC 9 cut(s) 44, 232, 484, 580, 1071, 1121, 1135, 1248, 1293
Mox20I TGGCCA 1 cut(s) 262
Mph1103I ATGCAT 1 cut(s) 738
MscI TGGCCA 1 cut(s) 262
MseI TTAA 6 cut(s) 597, 833, 978, 1304, 1397, 1403
Msp20I TGGCCA 1 cut(s) 262
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 3 cut(s) 11, 92, 280
MspR9I CCNGG 4 cut(s) 11, 126, 518, 903
MunI CAATTG 1 cut(s) 705
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 3 cut(s) 126, 518, 903
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 6 cut(s) 264, 268, 599, 742, 938, 1345
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 2 cut(s) 349, 396
NdeII GATC 3 cut(s) 86, 710, 1123
NlaIII CATG 7 cut(s) 267, 353, 400, 436, 569, 1221, 1292
NlaIV GGNNCC 3 cut(s) 673, 900, 1338
NsiI ATGCAT 1 cut(s) 738
NspI RCATGY 2 cut(s) 436, 569
NspV TTCGAA 1 cut(s) 579
PagI TCATGA 1 cut(s) 1288
PaqCI CACCTGC 1 cut(s) 1307
PctI GAATGC 1 cut(s) 736
PfeI GAWTC 2 cut(s) 219, 622
PfoI TCCNGGA 2 cut(s) 124, 901
PinAI ACCGGT 1 cut(s) 279
PkrI GCNGC 5 cut(s) 1015, 1084, 1153, 1198, 1347
PshAI GACNNNNGTC 1 cut(s) 888
PsiI TTATAA 1 cut(s) 1412
Psp124BI GAGCTC 2 cut(s) 430, 875
Psp1406I AACGTT 1 cut(s) 1176
Psp6I CCWGG 3 cut(s) 124, 516, 901
PspGI CCWGG 3 cut(s) 124, 516, 901
PspN4I GGNNCC 3 cut(s) 673, 900, 1338
PspPI GGNCC 3 cut(s) 128, 1211, 1337
PsuI RGATCY 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 3 cut(s) 284, 361, 1443
RsaNI GTAC 3 cut(s) 283, 360, 1442
SacI GAGCTC 2 cut(s) 430, 875
SaqAI TTAA 6 cut(s) 597, 833, 978, 1304, 1397, 1403
SatI GCNGC 5 cut(s) 1014, 1083, 1152, 1197, 1346
Sau3AI GATC 3 cut(s) 86, 710, 1123
Sau96I GGNCC 3 cut(s) 128, 1211, 1337
ScrFI CCNGG 4 cut(s) 11, 126, 518, 903
SduI GDGCHC 4 cut(s) 273, 430, 875, 1111
SfaNI GCATC 1 cut(s) 821
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 1 cut(s) 128
SsiI CCGC 6 cut(s) 45, 254, 726, 743, 1018, 1214
SspMI CTAG 5 cut(s) 389, 488, 746, 929, 1155
SstI GAGCTC 2 cut(s) 430, 875
StyD4I CCNGG 4 cut(s) 9, 124, 516, 901
StyI CCWWGG 2 cut(s) 349, 396
TaaI ACNGT 7 cut(s) 139, 441, 858, 946, 1073, 1333, 1408
TaiI ACGT 2 cut(s) 973, 1179
TaqI TCGA 3 cut(s) 157, 201, 579
TaqII GACCGA 1 cut(s) 145
TatI WGTACW 1 cut(s) 359
TfiI GAWTC 2 cut(s) 219, 622
Tru1I TTAA 6 cut(s) 597, 833, 978, 1304, 1397, 1403
Tru9I TTAA 6 cut(s) 597, 833, 978, 1304, 1397, 1403
TscAI CASTG 7 cut(s) 120, 861, 882, 949, 1092, 1111, 1338
TseI GCWGC 5 cut(s) 1013, 1082, 1151, 1196, 1345
TspDTI ATGAA 6 cut(s) 513, 816, 899, 1365, 1368, 1421
TspRI CASTG 7 cut(s) 120, 861, 882, 949, 1092, 1111, 1338
VneI GTGCAC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 7 cut(s) 119, 195, 478, 614, 829, 922, 1428
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 2 cut(s) 436, 569
XspI CTAG 5 cut(s) 389, 488, 746, 929, 1155
Zsp2I ATGCAT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.