MD02G1018100.v1.1

Calcium uptake protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005926
Physical Location & Seq
Forward (+)
1339231 .. 1341639
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1018100.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGTTTTCACGGGCATCTTCGAGGGGATCCTCAGGGTCCATCCACCGGCTTTTTTCGGATGTTAAAAGCCTCAGCTCTCGTCCTCCATTCAACAAATCGCAACCCTCCTCCTCGGTCGCTTCATCGCCATCTTCTTCTTCTTCTTCACTCAGCTCATCGGTTGGCAGATTTGGCTATGGTGGCAGTCATAATGGTAGATCAGGCTCCGATAACACTTTTGATTTGTTGCTGAGATCCGTTTCTTCGGGGGTTCTGATTCTCGGGACCAGTCTAGGGTTTTCGTATTGGTCTTCTTCTTCTCTGGATCCAAATTCCTACGTTTCTTACGCTGATAATTATCCCAAGGAGGCGGCATTTGCAATCGGCGAAGATACAAACAAAACTCAGTTTTCCCTCTTTGATGTTTCATATAGGAGAAGAAAATTCTTTACGTATGAAAAGCGGCTCCGAGATCAAAGTCCTCCGGAGAAGCTATTCGACTACTTTGCATCGGTTAAAAACCCCAACGGAGAGGCGTTTATGACGCCGGCAGACTTGATGAGAGCGGTGGTTCCTGTATTTCCTCCCTCAGAATCAACCCACGTTAGAGTGGGGTCTCTGAGAGGGGAGAAGGTTCCCAGCAAGCAGCAGTACGCTCCTTCCAAATTCTTTATGCTTTTCGACACTGACGGAGATGGCCTGATTTCCTTCCCGGAGTTCATCTTCTTCGTCAACCTCCTCAGCATCCCTGAATCGAGCTTTACGATTGCGTTTAAAATGTTTGATCTCGACAACAGCGGAGACATTGATAAGCTGGAGTTTAATAAAGTCATGTCCTTGATGCGATCTCAAAGCAAACAAAACACGGATAGCCACAGGCTCAATGTTTCTGTCGAAGACGGAGGCCTACTCGAGTACTTCTTCGGCAAAGACGGCAAAAAAAGTCTAAAACTTGAAAGATTTGTTCAGTTTTTCCGGGATTTACACGAAGAAATTTTGCGATTGGAGTTTGCTCACTACGACTTCAGAGCACAAGGAACCATATTGGCTAAAGACTTCGCGTTCTCCTTGGTTGCATCTGCTGATAGCGGTGAAATAAACAAGTTGCTTGATCGGGTGGACGAAATTGATAATGACGCACGTCTTAAGAACATAAGAATCACATACAAGGAATTCAAAGATTTCGCGGAGCTCCGTAAGAACTTGCAGTCCTTCTCTCTGGCCCTTTTCAGTTACGGAGAAGTTAACGGAGTGTTGACGAAAAGTGATCTCCAGAGAGCTGCTTCCCGAGTATGTGGCATCTCTGTTTCTACCAATGTGCTTGACATAATATTCCATCTATTCGATGCAAATGGTGACGGAGATTTAAGCGCAAATGAGTTCGTGAGAGTTTTACAAAGTCGGGGAGGCGAAACGCAAGAGACAGGCATAAGGGGACTCCTATCTTGCTGGTTTAATTGTGCTGGTAAAGCTTTTGGTCAGAACAACTGCAGCTCTGGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

495

Amino Acids

54.93

Weight (kDa)

6.69

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 409 - 460 6.6e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 545
AccII CGCG 2 cut(s) 1040, 1166
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 6 cut(s) 350, 442, 545, 777, 1068, 1166
AclWI GGATC 5 cut(s) 21, 34, 228, 299, 312
AcsI RAATTY 5 cut(s) 310, 422, 644, 972, 1151
AcuI CTGAAG 1 cut(s) 988
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 2 cut(s) 632, 896
AfiI CCNNNNNNNGG 2 cut(s) 45, 273
AflII CTTAAG 1 cut(s) 1124
AgsI TTSAA 3 cut(s) 91, 935, 1156
AjiI CACGTC 1 cut(s) 1121
AloI GAACNNNNNNTCC 2 cut(s) 1172, 1204
AluBI AGCT 9 cut(s) 75, 153, 472, 738, 793, 1171, 1259, 1451, 1473
AluI AGCT 9 cut(s) 75, 153, 472, 738, 793, 1171, 1259, 1451, 1473
Alw21I GWGCWC 2 cut(s) 1012, 1173
Alw26I GTCTC 3 cut(s) 600, 774, 1394
AlwI GGATC 5 cut(s) 21, 34, 228, 299, 312
Ama87I CYCGRG 3 cut(s) 260, 890, 1266
Aor13HI TCCGGA 1 cut(s) 463
AoxI GGCC 3 cut(s) 676, 883, 1200
ApeKI GCWGC 3 cut(s) 625, 1259, 1470
ApoI RAATTY 5 cut(s) 310, 422, 644, 972, 1151
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 1 cut(s) 1352
AspS9I GGNCC 3 cut(s) 36, 264, 1201
AsuC2I CCSGG 2 cut(s) 692, 956
AsuHPI GGTGA 2 cut(s) 1082, 1346
AvaI CYCGRG 3 cut(s) 260, 890, 1266
AvaII GGWCC 2 cut(s) 36, 264
AxyI CCTNAGG 1 cut(s) 31
BamHI GGATCC 2 cut(s) 26, 304
BanII GRGCYC 1 cut(s) 1173
BbsI GAAGAC 2 cut(s) 282, 882
Bbv12I GWGCWC 2 cut(s) 1012, 1173
BbvCI CCTCAGC 2 cut(s) 71, 719
BbvI GCAGC 2 cut(s) 637, 1246
BccI CCATC 4 cut(s) 47, 136, 668, 1323
BceAI ACGGC 1 cut(s) 928
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BcnI CCSGG 2 cut(s) 692, 956
BcoDI GTCTC 3 cut(s) 600, 774, 1394
BfaI CTAG 1 cut(s) 272
BfmI CTRYAG 1 cut(s) 1468
BfrI CTTAAG 1 cut(s) 1124
BisI GCNGC 5 cut(s) 351, 443, 626, 1260, 1471
BlsI GCNGC 5 cut(s) 352, 444, 627, 1261, 1472
BmcAI AGTACT 1 cut(s) 896
Bme1390I CCNGG 2 cut(s) 692, 956
Bme18I GGWCC 2 cut(s) 36, 264
BmeT110I CYCGRG 3 cut(s) 260, 890, 1266
BmgBI CACGTC 1 cut(s) 1121
BmgT120I GGNCC 3 cut(s) 36, 264, 1201
BmiI GGNNCC 9 cut(s) 28, 37, 205, 265, 306, 446, 552, 615, 1018
BmrFI CCNGG 2 cut(s) 692, 956
BmsI GCATC 7 cut(s) 23, 497, 732, 810, 1064, 1287, 1315
BoxI GACNNNNGTC 1 cut(s) 1119
BpiI GAAGAC 2 cut(s) 282, 882
BplI GAGNNNNNCTC 2 cut(s) 873, 905
BpmI CTGGAG 2 cut(s) 815, 1235
Bpu10I CCTNAGC 2 cut(s) 71, 719
BpuMI CCSGG 2 cut(s) 692, 956
BsaAI YACGTR 1 cut(s) 432
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 524
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 3 cut(s) 111, 342, 1047
BsaWI WCCGGW 1 cut(s) 463
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 273
Bse118I RCCGGY 2 cut(s) 45, 526
Bse1I ACTGG 1 cut(s) 267
Bse21I CCTNAGG 1 cut(s) 31
Bse8I GATNNNNATC 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 463
BseDI CCNNGG 3 cut(s) 111, 342, 1047
BseGI GGATG 3 cut(s) 39, 64, 723
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 45, 273
BseMII CTCAG 8 cut(s) 45, 85, 163, 221, 398, 582, 590, 733
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 3 cut(s) 97, 100, 707
BseXI GCAGC 2 cut(s) 637, 1246
BseYI CCCAGC 1 cut(s) 617
Bsh1236I CGCG 2 cut(s) 1040, 1166
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 3 cut(s) 678, 885, 1202
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 1012, 1173
BsiHKCI CYCGRG 3 cut(s) 260, 890, 1266
BsiSI CCGG 5 cut(s) 46, 464, 527, 692, 955
BslFI GGGAC 2 cut(s) 277, 1428
BslI CCNNNNNNNGG 2 cut(s) 45, 273
BsmAI GTCTC 3 cut(s) 600, 774, 1394
BsmFI GGGAC 2 cut(s) 277, 1428
BsnI GGCC 3 cut(s) 678, 885, 1202
Bso31I GGTCTC 1 cut(s) 600
BsoBI CYCGRG 3 cut(s) 260, 890, 1266
Bsp1286I GDGCHC 2 cut(s) 1012, 1173
Bsp13I TCCGGA 1 cut(s) 463
Bsp143I GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
BspACI CCGC 6 cut(s) 350, 442, 545, 777, 1068, 1166
BspANI GGCC 3 cut(s) 678, 885, 1202
BspCNI CTCAG 8 cut(s) 44, 84, 162, 222, 397, 581, 591, 732
BspEI TCCGGA 1 cut(s) 463
BspFNI CGCG 2 cut(s) 1040, 1166
BspLI GGNNCC 9 cut(s) 28, 37, 205, 265, 306, 446, 552, 615, 1018
BspMAI CTGCAG 1 cut(s) 1472
BspPI GGATC 5 cut(s) 21, 34, 228, 299, 312
BspTI CTTAAG 1 cut(s) 1124
BspTNI GGTCTC 1 cut(s) 600
BsrBI CCGCTC 1 cut(s) 545
BsrFI RCCGGY 2 cut(s) 45, 526
BsrI ACTGG 1 cut(s) 267
BssAI RCCGGY 2 cut(s) 45, 526
BssECI CCNNGG 3 cut(s) 111, 342, 1047
BssMI GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
BssNI GRCGYC 1 cut(s) 524
BssT1I CCWWGG 2 cut(s) 342, 1047
BstACI GRCGYC 1 cut(s) 524
BstAFI CTTAAG 1 cut(s) 1124
BstBAI YACGTR 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 528, 623
BstDEI CTNAG 8 cut(s) 31, 71, 149, 230, 384, 568, 599, 719
BstF5I GGATG 3 cut(s) 39, 64, 723
BstFNI CGCG 2 cut(s) 1040, 1166
BstHHI GCGC 1 cut(s) 1352
BstKTI GATC 9 cut(s) 29, 200, 236, 307, 454, 766, 827, 1093, 1249
BstMAI GTCTC 3 cut(s) 600, 774, 1394
BstMBI GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 5 cut(s) 171, 180, 356, 912, 1448
BstPAI GACNNNNGTC 1 cut(s) 1119
BstSCI CCNGG 2 cut(s) 690, 954
BstSFI CTRYAG 1 cut(s) 1468
BstSNI TACGTA 1 cut(s) 432
BstUI CGCG 2 cut(s) 1040, 1166
BstV1I GCAGC 2 cut(s) 637, 1246
BstV2I GAAGAC 2 cut(s) 282, 882
BstX2I RGATCY 3 cut(s) 26, 233, 304
BstYI RGATCY 3 cut(s) 26, 233, 304
Bsu36I CCTNAGG 1 cut(s) 31
BsuRI GGCC 3 cut(s) 678, 885, 1202
BtgZI GCGATG 1 cut(s) 108
BtrI CACGTC 1 cut(s) 1121
BtsCI GGATG 3 cut(s) 39, 64, 723
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 528, 623
CfoI GCGC 1 cut(s) 1352
Cfr10I RCCGGY 2 cut(s) 45, 526
Cfr13I GGNCC 3 cut(s) 36, 264, 1201
CseI GACGC 2 cut(s) 532, 1124
Csp6I GTAC 2 cut(s) 631, 895
CviAII CATG 1 cut(s) 811
CviQI GTAC 2 cut(s) 631, 895
DdeI CTNAG 8 cut(s) 31, 71, 149, 230, 384, 568, 599, 719
DpnI GATC 9 cut(s) 28, 199, 235, 306, 453, 765, 826, 1092, 1248
DpnII GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
DraI TTTAAA 1 cut(s) 754
Ecl136II GAGCTC 1 cut(s) 1171
Eco105I TACGTA 1 cut(s) 432
Eco130I CCWWGG 2 cut(s) 342, 1047
Eco147I AGGCCT 1 cut(s) 885
Eco24I GRGCYC 1 cut(s) 1173
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 36, 264
Eco53kI GAGCTC 1 cut(s) 1171
Eco57I CTGAAG 1 cut(s) 988
Eco81I CCTNAGG 1 cut(s) 31
Eco88I CYCGRG 3 cut(s) 260, 890, 1266
EcoICRI GAGCTC 1 cut(s) 1171
EcoRI GAATTC 1 cut(s) 1151
EcoT14I CCWWGG 2 cut(s) 342, 1047
EcoT38I GRGCYC 1 cut(s) 1173
ErhI CCWWGG 2 cut(s) 342, 1047
FaeI CATG 1 cut(s) 814
FaqI GGGAC 2 cut(s) 277, 1428
FatI CATG 1 cut(s) 810
Fnu4HI GCNGC 5 cut(s) 351, 443, 626, 1260, 1471
FokI GGATG 3 cut(s) 26, 71, 710
FriOI GRGCYC 1 cut(s) 1173
Fsp4HI GCNGC 5 cut(s) 351, 443, 626, 1260, 1471
FspBI CTAG 1 cut(s) 272
GlaI GCGC 1 cut(s) 1351
GluI GCNGC 5 cut(s) 351, 443, 626, 1260, 1471
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 2 cut(s) 815, 1235
HaeIII GGCC 3 cut(s) 678, 885, 1202
HapII CCGG 5 cut(s) 46, 464, 527, 692, 955
HgaI GACGC 2 cut(s) 532, 1124
HhaI GCGC 1 cut(s) 1352
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 1 cut(s) 814
Hin6I GCGC 1 cut(s) 1350
HinP1I GCGC 1 cut(s) 1350
HincII GTYRAC 3 cut(s) 712, 1225, 1236
HindII GTYRAC 3 cut(s) 712, 1225, 1236
HindIII AAGCTT 1 cut(s) 1449
HinfI GANTC 5 cut(s) 256, 572, 731, 1137, 1416
HpaI GTTAAC 1 cut(s) 1225
HpaII CCGG 5 cut(s) 46, 464, 527, 692, 955
HphI GGTGA 2 cut(s) 1082, 1346
Hpy166II GTNNAC 4 cut(s) 712, 1099, 1225, 1236
Hpy188I TCNGA 8 cut(s) 58, 208, 255, 449, 571, 600, 1007, 1461
Hpy188III TCNNGA 7 cut(s) 262, 302, 464, 767, 1252, 1266, 1363
Hpy8I GTNNAC 4 cut(s) 712, 1099, 1225, 1236
HpyAV CCTTC 4 cut(s) 604, 648, 697, 1201
HpyCH4IV ACGT 4 cut(s) 318, 431, 582, 1120
HpyCH4V TGCA 6 cut(s) 359, 488, 1055, 1186, 1328, 1470
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 180, 356, 912, 1448
HpyF3I CTNAG 8 cut(s) 31, 71, 149, 230, 384, 568, 599, 719
HpySE526I ACGT 4 cut(s) 318, 431, 582, 1120
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 1 cut(s) 814
HspAI GCGC 1 cut(s) 1350
Kpn2I TCCGGA 1 cut(s) 463
KroI GCCGGC 1 cut(s) 526
KroNI GCCGGC 1 cut(s) 528
KspAI GTTAAC 1 cut(s) 1225
Kzo9I GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
LmnI GCTCC 5 cut(s) 209, 450, 640, 1168, 1176
Lsp1109I GCAGC 2 cut(s) 637, 1246
LweI GCATC 7 cut(s) 23, 497, 732, 810, 1064, 1287, 1315
MaeI CTAG 1 cut(s) 272
MaeII ACGT 4 cut(s) 318, 431, 582, 1120
MaeIII GTNAC 2 cut(s) 1211, 1334
MalI GATC 9 cut(s) 28, 199, 235, 306, 453, 765, 826, 1092, 1248
MbiI CCGCTC 1 cut(s) 545
MboI GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
MflI RGATCY 3 cut(s) 26, 233, 304
MhlI GDGCHC 2 cut(s) 1012, 1173
MluCI AATT 8 cut(s) 310, 334, 422, 644, 972, 1104, 1151, 1435
MlyI GAGTC 1 cut(s) 1410
MroI TCCGGA 1 cut(s) 463
MroNI GCCGGC 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 8 cut(s) 63, 495, 753, 801, 1125, 1224, 1346, 1434
MspA1I CMGCKG 1 cut(s) 777
MspCI CTTAAG 1 cut(s) 1124
MspI CCGG 5 cut(s) 46, 464, 527, 692, 955
MspR9I CCNGG 2 cut(s) 692, 956
MvnI CGCG 2 cut(s) 1040, 1166
MwoI GCNNNNNNNGC 5 cut(s) 171, 180, 356, 912, 1448
NaeI GCCGGC 1 cut(s) 528
NciI CCSGG 2 cut(s) 692, 956
NdeII GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
NgoMIV GCCGGC 1 cut(s) 526
NlaIII CATG 1 cut(s) 814
NlaIV GGNNCC 9 cut(s) 28, 37, 205, 265, 306, 446, 552, 615, 1018
NmuCI GTSAC 1 cut(s) 1334
PaeR7I CTCGAG 1 cut(s) 890
PceI AGGCCT 1 cut(s) 885
PcsI WCGNNNNNNNCGW 1 cut(s) 740
PdiI GCCGGC 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 4 cut(s) 256, 572, 731, 1137
PfoI TCCNGGA 2 cut(s) 690, 954
PkrI GCNGC 5 cut(s) 352, 444, 627, 1261, 1472
PleI GAGTC 1 cut(s) 1410
PpsI GAGTC 1 cut(s) 1410
Ppu21I YACGTR 1 cut(s) 432
PshAI GACNNNNGTC 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 1173
PspFI CCCAGC 1 cut(s) 617
PspN4I GGNNCC 9 cut(s) 28, 37, 205, 265, 306, 446, 552, 615, 1018
PspPI GGNCC 3 cut(s) 36, 264, 1201
PspXI VCTCGAGB 1 cut(s) 890
PstI CTGCAG 1 cut(s) 1472
PsuI RGATCY 3 cut(s) 26, 233, 304
RsaI GTAC 2 cut(s) 632, 896
RsaNI GTAC 2 cut(s) 631, 895
SacI GAGCTC 1 cut(s) 1173
SaqAI TTAA 8 cut(s) 63, 495, 753, 801, 1125, 1224, 1346, 1434
SatI GCNGC 5 cut(s) 351, 443, 626, 1260, 1471
Sau3AI GATC 9 cut(s) 26, 197, 233, 304, 451, 763, 824, 1090, 1246
Sau96I GGNCC 3 cut(s) 36, 264, 1201
ScaI AGTACT 1 cut(s) 896
SchI GAGTC 1 cut(s) 1410
ScrFI CCNGG 2 cut(s) 692, 956
SduI GDGCHC 2 cut(s) 1012, 1173
SfaNI GCATC 7 cut(s) 23, 497, 732, 810, 1064, 1287, 1315
SfcI CTRYAG 1 cut(s) 1468
Sfr274I CTCGAG 1 cut(s) 890
SinI GGWCC 2 cut(s) 36, 264
SlaI CTCGAG 1 cut(s) 890
SmlI CTYRAG 2 cut(s) 890, 1124
SmoI CTYRAG 2 cut(s) 890, 1124
SnaBI TACGTA 1 cut(s) 432
Sse9I AATT 8 cut(s) 310, 334, 422, 644, 972, 1104, 1151, 1435
SseBI AGGCCT 1 cut(s) 885
SsiI CCGC 6 cut(s) 350, 442, 545, 777, 1068, 1166
SspI AATATT 1 cut(s) 1311
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 1 cut(s) 1173
StuI AGGCCT 1 cut(s) 885
StyD4I CCNGG 2 cut(s) 690, 954
StyI CCWWGG 2 cut(s) 342, 1047
TaiI ACGT 4 cut(s) 321, 434, 585, 1123
TaqI TCGA 8 cut(s) 20, 477, 660, 734, 768, 873, 891, 1323
TaqII GACCGA 1 cut(s) 103
TasI AATT 8 cut(s) 310, 334, 422, 644, 972, 1104, 1151, 1435
TatI WGTACW 1 cut(s) 894
TauI GCSGC 2 cut(s) 353, 445
TfiI GAWTC 4 cut(s) 256, 572, 731, 1137
Tru1I TTAA 8 cut(s) 63, 495, 753, 801, 1125, 1224, 1346, 1434
Tru9I TTAA 8 cut(s) 63, 495, 753, 801, 1125, 1224, 1346, 1434
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 1334
TseI GCWGC 3 cut(s) 625, 1259, 1470
Tsp45I GTSAC 1 cut(s) 1334
TspDTI ATGAA 4 cut(s) 111, 396, 450, 688
TspGWI ACGGA 9 cut(s) 226, 522, 684, 860, 894, 1163, 1230, 1242, 1353
TspRI CASTG 1 cut(s) 670
Vha464I CTTAAG 1 cut(s) 1124
VpaK11BI GGWCC 2 cut(s) 36, 264
XapI RAATTY 5 cut(s) 310, 422, 644, 972, 1151
XhoI CTCGAG 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 1 cut(s) 272
ZrmI AGTACT 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.