Rorug01G0471500

Calcium uptake protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005926
Physical Location & Seq
Forward (+)
56247405 .. 56250287
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0471500.1

Sequence Viewer

Length: 1305 bp
ATGTATGTGATGACACCCATCACTTACTGGTTCAATATCTACAGCGCCAAGACATTCCCAATATTTTCTAGCAATTTGTTCATGGCTAATGGTTCAAGATACGAGGTCCTCGATATCATTGATTCCAGGTTTCAACTTGATCACGGCGAATATGCAAAGAAGGGGCCTGTGCATATGAGCACTTTTTTTGCTTTTACTTATGGTCTTGGATTTGCCACTCTTTCTGCTACAGTTATGCATGTTCTACTTTTTAATGCAAGGGATATACTGGACTCGTTTCGTGGAATTAAGAAAAAGGATGTCCATGCAAGGCTTATGAAGAGTTATAAATCAGTGCCAACATTGTGGTTTATCCTCATCCTTGTGATCAATATTGCTCTTATCCTGTTTGCTTGTGTGTATTACAAGGAGTCTCTTCAACTGCCTTGGTGGGGTGCATTGCTTGCTTGTTTCATGGCCGTTGTGTTCACGTACCCAATTGGTGTCATCCAAGCCACCACAAACCAGCAACCAGGTTTGAATATTATCACAGAATATGTGATTGGATACCTTTATCCTGAGCGCCCTGTAGCTAACATGTGCTTCAAAGTGTATGGTTACATAAGTATGATGCAAGGTCTAAATTTTGTGTCGGATTTTAAGCTCGGTCACTACATGAAGATTCCACCAAGAGCAATGTTCACAGCACAGACGGTAGGAACTGTTGTAGCTGTGGTTGTATACCAGTGTACAGCATGGTGGCTGATGGCAGACATTCCCAACCTCTGTGATACCTCCCTGCTGCCACCAGACAGCCCTTGGACTTGTCCACAGGACCGCGTGTTCTTTGATGCGTCTGTTATCTGGGGTCTCGTTGGACCACGGAGAATTTTTGGAAACCTCGGTGAATACAGTAACATCAACTGGTTTTTCCTTGGTGGCGCCATTGCCCCGGTGCTTGTTTGGCTTGCTCACAGGATATTTCCCAAACAAAAATGGATTCTTGCCGTTAACATGCCTGTCTTGCTAGGTGCCACGGCAATGATGCCCCCTGCCACTGCTTTGAACTTTACAAGTTGGATATTTGTGGCATTTCTTTCAGGTTTTGTTGTCTTCAGGTACCGGCAAGATTTGTGGAAGAAGTATAACTATGTCGCCTCTGGTGCTCTCGAGGCCGGAACTGCCTTTGTTACGATATTGGTGTTCATTACCTTGCAGTCCCAGAATATTGAACTCGACTGGTGGGGGAACAAAGGAGAGCTCTGCCCCTTGGCTACATGCCCTACTGCGAAAGGGGTTGTCATTGCTGGCTGCCCGGTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

434

Amino Acids

48.99

Weight (kDa)

8.91

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 2 - 396 5.4e-94 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
Acc65I GGTACC 1 cut(s) 1098
AccB1I GGYRCC 3 cut(s) 920, 1010, 1098
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 819
AciI CCGC 1 cut(s) 817
AcoI YGGCCR 1 cut(s) 456
AcsI RAATTY 2 cut(s) 622, 867
AcuI CTGAAG 1 cut(s) 1078
AcyI GRCGYC 1 cut(s) 921
AfaI GTAC 3 cut(s) 473, 730, 1100
AfiI CCNNNNNNNGG 1 cut(s) 431
AflIII ACRYGT 1 cut(s) 576
AgsI TTSAA 8 cut(s) 34, 96, 134, 419, 520, 586, 1045, 1211
AjnI CCWGG 2 cut(s) 125, 511
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AloI GAACNNNNNNTCC 2 cut(s) 806, 838
AluBI AGCT 4 cut(s) 572, 643, 710, 1240
AluI AGCT 4 cut(s) 572, 643, 710, 1240
Alw21I GWGCWC 3 cut(s) 182, 1147, 1242
Alw26I GTCTC 2 cut(s) 417, 854
Ama87I CYCGRG 1 cut(s) 1148
AoxI GGCC 3 cut(s) 164, 456, 1152
ApeKI GCWGC 2 cut(s) 781, 1290
ApoI RAATTY 2 cut(s) 622, 867
Asp718I GGTACC 1 cut(s) 1098
AspLEI GCGC 3 cut(s) 47, 564, 923
AspS9I GGNCC 5 cut(s) 106, 164, 814, 857, 1297
AsuC2I CCSGG 2 cut(s) 932, 1295
AsuHPI GGTGA 1 cut(s) 896
AvaI CYCGRG 1 cut(s) 1148
AvaII GGWCC 4 cut(s) 106, 814, 857, 1297
BanI GGYRCC 3 cut(s) 920, 1010, 1098
BanII GRGCYC 1 cut(s) 1242
BbsI GAAGAC 1 cut(s) 1084
Bbv12I GWGCWC 3 cut(s) 182, 1147, 1242
BbvI GCAGC 2 cut(s) 768, 1277
BccI CCATC 2 cut(s) 26, 739
BceAI ACGGC 4 cut(s) 160, 443, 971, 1032
BciT130I CCWGG 2 cut(s) 127, 513
BciVI GTATCC 1 cut(s) 539
BclI TGATCA 2 cut(s) 139, 366
BcnI CCSGG 2 cut(s) 932, 1295
BcoDI GTCTC 2 cut(s) 417, 854
BfaI CTAG 2 cut(s) 69, 1007
BfmI CTRYAG 3 cut(s) 40, 228, 567
BfoI RGCGCY 3 cut(s) 48, 565, 924
BfuI GTATCC 1 cut(s) 539
BisI GCNGC 2 cut(s) 782, 1291
BlsI GCNGC 2 cut(s) 783, 1292
Bme1390I CCNGG 4 cut(s) 127, 513, 932, 1295
Bme18I GGWCC 4 cut(s) 106, 814, 857, 1297
BmeT110I CYCGRG 1 cut(s) 1148
BmgT120I GGNCC 5 cut(s) 106, 164, 814, 857, 1297
BmiI GGNNCC 4 cut(s) 165, 922, 1012, 1100
BmrFI CCNGG 4 cut(s) 127, 513, 932, 1295
BmsI GCATC 3 cut(s) 600, 820, 1014
BpiI GAAGAC 1 cut(s) 1084
Bpu10I CCTNAGC 1 cut(s) 558
BpuMI CCSGG 2 cut(s) 932, 1295
BsaAI YACGTR 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 921
BsaI GGTCTC 1 cut(s) 854
BsaJI CCNNGG 8 cut(s) 425, 797, 860, 880, 913, 930, 1014, 1248
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse118I RCCGGY 1 cut(s) 1101
Bse1I ACTGG 5 cut(s) 32, 273, 724, 908, 1223
Bse3DI GCAATG 5 cut(s) 437, 681, 924, 1026, 1281
BseBI CCWGG 2 cut(s) 127, 513
BseDI CCNNGG 8 cut(s) 425, 797, 860, 880, 913, 930, 1014, 1248
BseGI GGATG 3 cut(s) 304, 357, 486
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMI GCAATG 5 cut(s) 437, 681, 924, 1026, 1281
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 5 cut(s) 32, 273, 724, 908, 1223
BseXI GCAGC 2 cut(s) 768, 1277
Bsh1236I CGCG 1 cut(s) 819
BshFI GGCC 3 cut(s) 166, 458, 1154
BshNI GGYRCC 3 cut(s) 920, 1010, 1098
BsiHKAI GWGCWC 3 cut(s) 182, 1147, 1242
BsiHKCI CYCGRG 1 cut(s) 1148
BsiSI CCGG 4 cut(s) 932, 1102, 1155, 1295
BslFI GGGAC 1 cut(s) 1183
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 2 cut(s) 417, 854
BsmFI GGGAC 1 cut(s) 1183
BsnI GGCC 3 cut(s) 166, 458, 1154
Bso31I GGTCTC 1 cut(s) 854
BsoBI CYCGRG 1 cut(s) 1148
Bsp1286I GDGCHC 3 cut(s) 182, 1147, 1242
Bsp1407I TGTACA 1 cut(s) 728
Bsp143I GATC 2 cut(s) 139, 366
BspACI CCGC 1 cut(s) 817
BspANI GGCC 3 cut(s) 166, 458, 1154
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 1 cut(s) 819
BspLI GGNNCC 4 cut(s) 165, 922, 1012, 1100
BspT107I GGYRCC 3 cut(s) 920, 1010, 1098
BspTNI GGTCTC 1 cut(s) 854
BsrDI GCAATG 5 cut(s) 437, 681, 924, 1026, 1281
BsrFI RCCGGY 1 cut(s) 1101
BsrGI TGTACA 1 cut(s) 728
BsrI ACTGG 5 cut(s) 32, 273, 724, 908, 1223
BssAI RCCGGY 1 cut(s) 1101
BssECI CCNNGG 8 cut(s) 425, 797, 860, 880, 913, 930, 1014, 1248
BssMI GATC 2 cut(s) 139, 366
BssNAI GTATAC 1 cut(s) 721
BssNI GRCGYC 1 cut(s) 921
BssT1I CCWWGG 4 cut(s) 425, 797, 913, 1248
Bst1107I GTATAC 1 cut(s) 721
Bst2UI CCWGG 2 cut(s) 127, 513
Bst4CI ACNGT 4 cut(s) 232, 694, 703, 893
Bst6I CTCTTC 2 cut(s) 314, 420
BstACI GRCGYC 1 cut(s) 921
BstAPI GCANNNNNTGC 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 728
BstBAI YACGTR 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 444, 948, 1288
BstDEI CTNAG 1 cut(s) 558
BstDSI CCRYGG 2 cut(s) 860, 1014
BstF5I GGATG 3 cut(s) 304, 357, 486
BstFNI CGCG 1 cut(s) 819
BstH2I RGCGCY 3 cut(s) 48, 565, 924
BstHHI GCGC 3 cut(s) 47, 564, 923
BstKTI GATC 2 cut(s) 142, 369
BstMAI GTCTC 2 cut(s) 417, 854
BstMBI GATC 2 cut(s) 139, 366
BstMWI GCNNNNNNNGC 6 cut(s) 443, 943, 1003, 1142, 1151, 1160
BstNI CCWGG 2 cut(s) 127, 513
BstNSI RCATGY 4 cut(s) 242, 580, 997, 1260
BstSCI CCNGG 4 cut(s) 125, 511, 930, 1293
BstSFI CTRYAG 3 cut(s) 40, 228, 567
BstUI CGCG 1 cut(s) 819
BstV1I GCAGC 2 cut(s) 768, 1277
BstV2I GAAGAC 1 cut(s) 1084
BstXI CCANNNNNNTGG 1 cut(s) 345
BstZ17I GTATAC 1 cut(s) 721
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 3 cut(s) 166, 458, 1154
BtgI CCRYGG 2 cut(s) 860, 1014
BtsCI GGATG 3 cut(s) 304, 357, 486
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 3 cut(s) 339, 731, 1035
Cac8I GCNNGC 3 cut(s) 444, 948, 1288
CfoI GCGC 3 cut(s) 47, 564, 923
Cfr10I RCCGGY 1 cut(s) 1101
Cfr13I GGNCC 5 cut(s) 106, 164, 814, 857, 1297
CseI GACGC 1 cut(s) 822
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 3 cut(s) 472, 729, 1099
CspCI CAANNNNNGTGG 2 cut(s) 1094, 1129
CviAII CATG 9 cut(s) 82, 239, 305, 454, 577, 655, 735, 994, 1257
CviQI GTAC 3 cut(s) 472, 729, 1099
DdeI CTNAG 1 cut(s) 558
DinI GGCGCC 1 cut(s) 922
DpnI GATC 2 cut(s) 141, 368
DpnII GATC 2 cut(s) 139, 366
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 2 cut(s) 314, 420
EarI CTCTTC 2 cut(s) 314, 420
Ecl136II GAGCTC 1 cut(s) 1240
Eco130I CCWWGG 4 cut(s) 425, 797, 913, 1248
Eco24I GRGCYC 1 cut(s) 1242
Eco31I GGTCTC 1 cut(s) 854
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 4 cut(s) 106, 814, 857, 1297
Eco53kI GAGCTC 1 cut(s) 1240
Eco57I CTGAAG 1 cut(s) 1078
Eco88I CYCGRG 1 cut(s) 1148
EcoICRI GAGCTC 1 cut(s) 1240
EcoO109I RGGNCCY 2 cut(s) 106, 164
EcoRII CCWGG 2 cut(s) 125, 511
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 4 cut(s) 425, 797, 913, 1248
EcoT22I ATGCAT 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 1242
EgeI GGCGCC 1 cut(s) 922
EheI GGCGCC 1 cut(s) 922
ErhI CCWWGG 4 cut(s) 425, 797, 913, 1248
FaeI CATG 9 cut(s) 85, 242, 308, 457, 580, 658, 738, 997, 1260
FaqI GGGAC 1 cut(s) 1183
FatI CATG 9 cut(s) 81, 238, 304, 453, 576, 654, 734, 993, 1256
FauNDI CATATG 1 cut(s) 174
FbaI TGATCA 2 cut(s) 139, 366
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 2 cut(s) 782, 1291
FokI GGATG 3 cut(s) 311, 344, 473
FriOI GRGCYC 1 cut(s) 1242
Fsp4HI GCNGC 2 cut(s) 782, 1291
FspBI CTAG 2 cut(s) 69, 1007
GlaI GCGC 3 cut(s) 46, 563, 922
GluI GCNGC 2 cut(s) 782, 1291
HaeII RGCGCY 3 cut(s) 48, 565, 924
HaeIII GGCC 3 cut(s) 166, 458, 1154
HapII CCGG 4 cut(s) 932, 1102, 1155, 1295
HgaI GACGC 1 cut(s) 822
HhaI GCGC 3 cut(s) 47, 564, 923
Hin1I GRCGYC 1 cut(s) 921
Hin1II CATG 9 cut(s) 85, 242, 308, 457, 580, 658, 738, 997, 1260
Hin6I GCGC 3 cut(s) 45, 562, 921
HinP1I GCGC 3 cut(s) 45, 562, 921
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HinfI GANTC 5 cut(s) 122, 272, 410, 661, 979
HpaI GTTAAC 1 cut(s) 991
HpaII CCGG 4 cut(s) 932, 1102, 1155, 1295
HphI GGTGA 1 cut(s) 896
Hpy166II GTNNAC 6 cut(s) 468, 681, 721, 729, 809, 991
Hpy188I TCNGA 2 cut(s) 634, 1304
Hpy188III TCNNGA 3 cut(s) 96, 557, 1148
Hpy8I GTNNAC 6 cut(s) 468, 681, 721, 729, 809, 991
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 4 cut(s) 232, 694, 703, 893
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 8 cut(s) 155, 172, 238, 257, 308, 437, 613, 1195
HpyF10VI GCNNNNNNNGC 6 cut(s) 443, 943, 1003, 1142, 1151, 1160
HpyF3I CTNAG 1 cut(s) 558
HpySE526I ACGT 1 cut(s) 470
Hsp92I GRCGYC 1 cut(s) 921
Hsp92II CATG 9 cut(s) 85, 242, 308, 457, 580, 658, 738, 997, 1260
HspAI GCGC 3 cut(s) 45, 562, 921
KasI GGCGCC 1 cut(s) 920
KpnI GGTACC 1 cut(s) 1102
Ksp22I TGATCA 2 cut(s) 139, 366
KspAI GTTAAC 1 cut(s) 991
Kzo9I GATC 2 cut(s) 139, 366
Lsp1109I GCAGC 2 cut(s) 768, 1277
LweI GCATC 3 cut(s) 600, 820, 1014
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 2 cut(s) 69, 1007
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 4 cut(s) 596, 647, 893, 1168
MalI GATC 2 cut(s) 141, 368
MboI GATC 2 cut(s) 139, 366
MboII GAAGA 5 cut(s) 331, 407, 670, 1084, 1129
MfeI CAATTG 1 cut(s) 477
MhlI GDGCHC 3 cut(s) 182, 1147, 1242
MluCI AATT 5 cut(s) 73, 285, 477, 622, 867
Mly113I GGCGCC 1 cut(s) 921
MlyI GAGTC 2 cut(s) 266, 419
MmeI TCCRAC 3 cut(s) 612, 835, 1037
MnlI CCTC 8 cut(s) 97, 119, 365, 773, 784, 890, 1144, 1147
Mph1103I ATGCAT 1 cut(s) 240
MseI TTAA 4 cut(s) 252, 288, 639, 990
MslI CAYNNNNRTG 3 cut(s) 362, 605, 1019
MspI CCGG 4 cut(s) 932, 1102, 1155, 1295
MspR9I CCNGG 4 cut(s) 127, 513, 932, 1295
MunI CAATTG 1 cut(s) 477
MvaI CCWGG 2 cut(s) 127, 513
MvnI CGCG 1 cut(s) 819
MwoI GCNNNNNNNGC 6 cut(s) 443, 943, 1003, 1142, 1151, 1160
NarI GGCGCC 1 cut(s) 921
NciI CCSGG 2 cut(s) 932, 1295
NdeI CATATG 1 cut(s) 174
NdeII GATC 2 cut(s) 139, 366
NlaIII CATG 9 cut(s) 85, 242, 308, 457, 580, 658, 738, 997, 1260
NlaIV GGNNCC 4 cut(s) 165, 922, 1012, 1100
NmuCI GTSAC 1 cut(s) 647
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 4 cut(s) 242, 580, 997, 1260
PaeR7I CTCGAG 1 cut(s) 1148
PciI ACATGT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 3 cut(s) 122, 661, 979
PkrI GCNGC 2 cut(s) 783, 1292
PleI GAGTC 2 cut(s) 266, 418
PluTI GGCGCC 1 cut(s) 924
PpsI GAGTC 2 cut(s) 266, 418
Ppu21I YACGTR 1 cut(s) 471
PpuMI RGGWCCY 1 cut(s) 106
PscI ACATGT 1 cut(s) 576
PsiI TTATAA 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 1242
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 2 cut(s) 125, 511
PspGI CCWGG 2 cut(s) 125, 511
PspN4I GGNNCC 4 cut(s) 165, 922, 1012, 1100
PspPI GGNCC 5 cut(s) 106, 164, 814, 857, 1297
PspPPI RGGWCCY 1 cut(s) 106
RsaI GTAC 3 cut(s) 473, 730, 1100
RsaNI GTAC 3 cut(s) 472, 729, 1099
RseI CAYNNNNRTG 3 cut(s) 362, 605, 1019
SacI GAGCTC 1 cut(s) 1242
SaqAI TTAA 4 cut(s) 252, 288, 639, 990
SatI GCNGC 2 cut(s) 782, 1291
Sau3AI GATC 2 cut(s) 139, 366
Sau96I GGNCC 5 cut(s) 106, 164, 814, 857, 1297
SchI GAGTC 2 cut(s) 266, 419
ScrFI CCNGG 4 cut(s) 127, 513, 932, 1295
SduI GDGCHC 3 cut(s) 182, 1147, 1242
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 3 cut(s) 600, 820, 1014
SfcI CTRYAG 3 cut(s) 40, 228, 567
SfoI GGCGCC 1 cut(s) 922
Sfr274I CTCGAG 1 cut(s) 1148
SinI GGWCC 4 cut(s) 106, 814, 857, 1297
SlaI CTCGAG 1 cut(s) 1148
SmiMI CAYNNNNRTG 3 cut(s) 362, 605, 1019
SmlI CTYRAG 1 cut(s) 1148
SmoI CTYRAG 1 cut(s) 1148
Sse9I AATT 5 cut(s) 73, 285, 477, 622, 867
SsiI CCGC 1 cut(s) 817
SspDI GGCGCC 1 cut(s) 920
SspI AATATT 4 cut(s) 63, 373, 523, 1207
SspMI CTAG 2 cut(s) 69, 1007
SstI GAGCTC 1 cut(s) 1242
StyD4I CCNGG 4 cut(s) 125, 511, 930, 1293
StyI CCWWGG 4 cut(s) 425, 797, 913, 1248
TaaI ACNGT 4 cut(s) 232, 694, 703, 893
TaiI ACGT 1 cut(s) 473
TaqI TCGA 3 cut(s) 111, 1149, 1215
TaqII GACCGA 1 cut(s) 635
TasI AATT 5 cut(s) 73, 285, 477, 622, 867
TatI WGTACW 1 cut(s) 728
TfiI GAWTC 3 cut(s) 122, 661, 979
Tru1I TTAA 4 cut(s) 252, 288, 639, 990
Tru9I TTAA 4 cut(s) 252, 288, 639, 990
TscAI CASTG 3 cut(s) 339, 731, 1042
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 2 cut(s) 781, 1290
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 5 cut(s) 70, 332, 442, 671, 1174
TspGWI ACGGA 1 cut(s) 877
TspRI CASTG 3 cut(s) 339, 731, 1042
VpaK11BI GGWCC 4 cut(s) 106, 814, 857, 1297
XapI RAATTY 2 cut(s) 622, 867
XceI RCATGY 4 cut(s) 242, 580, 997, 1260
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XhoI CTCGAG 1 cut(s) 1148
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 2 cut(s) 69, 1007
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.