Rh2AG024300

Calcium uptake protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005926
Physical Location & Seq
Forward (+)
1711288 .. 1715680
4393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG024300.1

Sequence Viewer

Length: 1290 bp
ATGTTTTCTTGGGCATCTTCAAGAAGAACTTCAGGGGTTGTTTTGGGTGTTCAACGACTCGGCTCTCGCACATTGTTCAGTAAATCACAACCTTCATCATCGCCATCTTCGTCTTCTTCGATCTCATCATGTGGTGGCAGTTTTGGCTGTAATAATGAGTACAATCACAAGAGTAGATCAGGCTCCTCCAATAAGGGTAGTAAATTTGATTTGATTCAGAGGTCAGTTTCTTCAGGGGTTCTAATTCTCGGGTCAAGCATAGGCTATTCTTATTGGTCTTCTTCTACCTTGGATCCAAACGCCTCTGTTTGTTTTGCTGATTCCAAAGAGGAGGCAGCATGGGCAATCGAGCAGGACCCTGCAGAACAATTCAAGTCCCTCTTTTCAAATACATTTAGGAGAAAAAAGTTCTTTACGTATGAAAAAAAGATAAGAGAGCGAAGTACCCCTGAAAAGATTTTCAATTACTTTGCATCAGTTCGAACCCCTAGCGGAGAGTGCTTTATGACTCCGGGGGACTTGATGCGAGCTGTTGTTCCTGTGTTTCCTCCCTATGATTCAAATCGGGTTCGAGAAGGCTCTTTAAGAGGGGAAAATGTTCCTGGAGAATTGCAATGTGCTCCTTCTAAATTCTTCATGCTTTTCGACACTGATGGTGATGGCCTCATTTCATTTCAAGAGTACATGTTTTTCGTCACTCTCCTCAGCATTCCTGAATCGAGTTTTACTGTGGCATTTAAAATGTTCGATCTTGACAACAGTGGAGACATTGATAAGGAAGAGTTTAACAGAGTTATGGCATTGATGCGAAATCACAACAATAAAATCAAGGACGGCCACAGGGTCAAAGTTGGTGTAGAAGAGGGAGGAATACTCGAGTACTTCTTCGGCAAAGACGGCAAAAAAGGCCTTAAGCATGAAAGATTTGTTCAGTTTTTCAGGGATTTACACGAGGAAATGTTGCGCTTGGAGTTTGCTCATTATGACTACAAATCAAAAGGAACCATAACAGCTAAAGATTTTGCCTTGTCCTTGGTTGCATCTGCTGATCTCAGTGACTTATACAAGTTGCTTGATCGTGTTGACGATATTAACAACGCCCCACGTCTTAACAACATACAAATTACATACAAGGAATTTAAAGATTTTGCAGAGCTACGCAAGAATTTGCAGTCTTTCTCTTTGGCCATTTTCAGCTACGGCGAGGTTAATGGGGTTTTGACAAAATCAGATCTCCAGAGAGCAGCTTCCAAAGTAATTCTCACATACCAATCTATCTGTTCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

429

Amino Acids

48.32

Weight (kDa)

8.54

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_5 PF13202 245 - 266 2.3e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 492
AclWI GGATC 2 cut(s) 287, 300
AcoI YGGCCR 2 cut(s) 835, 1185
AcsI RAATTY 4 cut(s) 203, 629, 1136, 1165
AcuI CTGAAG 2 cut(s) 15, 216
AfaI GTAC 4 cut(s) 161, 445, 683, 881
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 7 cut(s) 21, 53, 373, 387, 463, 561, 677
AjiI CACGTC 1 cut(s) 1106
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 5 cut(s) 530, 1013, 1156, 1197, 1247
AluI AGCT 5 cut(s) 530, 1013, 1156, 1197, 1247
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 1 cut(s) 759
AlwI GGATC 2 cut(s) 287, 300
Ama87I CYCGRG 2 cut(s) 248, 875
AoxI GGCC 4 cut(s) 661, 835, 907, 1185
ApeKI GCWGC 2 cut(s) 335, 1244
ApoI RAATTY 4 cut(s) 203, 629, 1136, 1165
Asp700I GAANNNNTTC 2 cut(s) 28, 597
AspLEI GCGC 1 cut(s) 966
AspS9I GGNCC 1 cut(s) 355
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 1 cut(s) 668
AsuII TTCGAA 1 cut(s) 481
AvaI CYCGRG 2 cut(s) 248, 875
AvaII GGWCC 1 cut(s) 355
BalI TGGCCA 1 cut(s) 1187
BamHI GGATCC 1 cut(s) 292
BauI CACGAG 1 cut(s) 950
BbsI GAAGAC 2 cut(s) 105, 270
Bbv12I GWGCWC 1 cut(s) 622
BbvCI CCTCAGC 1 cut(s) 704
BbvI GCAGC 2 cut(s) 347, 1256
BccI CCATC 3 cut(s) 112, 647, 653
BceAI ACGGC 3 cut(s) 850, 913, 1216
BciT130I CCWGG 1 cut(s) 603
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 759
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 1 cut(s) 360
BfrI CTTAAG 1 cut(s) 911
BglII AGATCT 1 cut(s) 1231
BisI GCNGC 2 cut(s) 336, 1245
BlsI GCNGC 2 cut(s) 337, 1246
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 2 cut(s) 513, 603
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 248, 875
BmgBI CACGTC 1 cut(s) 1106
BmgT120I GGNCC 1 cut(s) 355
BmiI GGNNCC 4 cut(s) 184, 294, 357, 1003
BmrFI CCNGG 2 cut(s) 513, 603
BmsI GCATC 5 cut(s) 23, 482, 513, 795, 1049
BpiI GAAGAC 2 cut(s) 105, 270
BplI GAGNNNNNCTC 2 cut(s) 858, 890
BpmI CTGGAG 2 cut(s) 624, 1220
Bpu10I CCTNAGC 1 cut(s) 704
Bpu14I TTCGAA 1 cut(s) 481
BpuMI CCSGG 1 cut(s) 513
BsaAI YACGTR 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 288, 512, 1032
Bse3DI GCAATG 1 cut(s) 620
Bse8I GATNNNNATC 1 cut(s) 561
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 3 cut(s) 288, 512, 1032
BseJI GATNNNNATC 1 cut(s) 561
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 2 cut(s) 718, 1066
BseRI GAGGAG 3 cut(s) 175, 344, 692
BseXI GCAGC 2 cut(s) 347, 1256
BshFI GGCC 4 cut(s) 663, 837, 909, 1187
BsiHKAI GWGCWC 1 cut(s) 622
BsiHKCI CYCGRG 2 cut(s) 248, 875
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 2 cut(s) 361, 530
BsmAI GTCTC 1 cut(s) 759
BsmFI GGGAC 2 cut(s) 361, 530
BsmI GAATGC 1 cut(s) 708
BsnI GGCC 4 cut(s) 663, 837, 909, 1187
BsoBI CYCGRG 2 cut(s) 248, 875
Bsp119I TTCGAA 1 cut(s) 481
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
BspACI CCGC 1 cut(s) 492
BspANI GGCC 4 cut(s) 663, 837, 909, 1187
BspCNI CTCAG 2 cut(s) 717, 1065
BspLI GGNNCC 4 cut(s) 184, 294, 357, 1003
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 2 cut(s) 287, 300
BspT104I TTCGAA 1 cut(s) 481
BspTI CTTAAG 1 cut(s) 911
BsrDI GCAATG 1 cut(s) 620
BssECI CCNNGG 3 cut(s) 288, 512, 1032
BssMI GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
BssSI CACGAG 1 cut(s) 950
BssT1I CCWWGG 2 cut(s) 288, 1032
Bst2BI CACGAG 1 cut(s) 950
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 2 cut(s) 730, 761
Bst6I CTCTTC 2 cut(s) 774, 855
BstAFI CTTAAG 1 cut(s) 911
BstBAI YACGTR 1 cut(s) 417
BstBI TTCGAA 1 cut(s) 481
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 704, 1052
BstHHI GCGC 1 cut(s) 966
BstKTI GATC 7 cut(s) 123, 179, 295, 751, 1051, 1078, 1234
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
BstMWI GCNNNNNNNGC 5 cut(s) 144, 341, 498, 897, 906
BstNI CCWGG 1 cut(s) 603
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 2 cut(s) 511, 601
BstSFI CTRYAG 1 cut(s) 360
BstSNI TACGTA 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 347, 1256
BstV2I GAAGAC 2 cut(s) 105, 270
BstX2I RGATCY 2 cut(s) 292, 1231
BstYI RGATCY 2 cut(s) 292, 1231
BsuRI GGCC 4 cut(s) 663, 837, 909, 1187
BtgZI GCGATG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 1106
BtsIMutI CAGTG 3 cut(s) 648, 766, 1060
Cac8I GCNNGC 1 cut(s) 528
CfoI GCGC 1 cut(s) 966
Cfr13I GGNCC 1 cut(s) 355
Csp6I GTAC 4 cut(s) 160, 444, 682, 880
CviAII CATG 5 cut(s) 129, 339, 637, 685, 917
CviQI GTAC 4 cut(s) 160, 444, 682, 880
DdeI CTNAG 2 cut(s) 704, 1052
DpnI GATC 7 cut(s) 122, 178, 294, 750, 1050, 1077, 1233
DpnII GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
DraI TTTAAA 2 cut(s) 739, 1141
EaeI YGGCCR 2 cut(s) 835, 1185
Eam1104I CTCTTC 2 cut(s) 774, 855
EarI CTCTTC 2 cut(s) 774, 855
Eco105I TACGTA 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 288, 1032
Eco147I AGGCCT 1 cut(s) 909
Eco47I GGWCC 1 cut(s) 355
Eco57I CTGAAG 2 cut(s) 15, 216
Eco88I CYCGRG 2 cut(s) 248, 875
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 2 cut(s) 288, 1032
ErhI CCWWGG 2 cut(s) 288, 1032
FaeI CATG 5 cut(s) 132, 342, 640, 688, 920
FalI AAGNNNNNCTT 4 cut(s) 13, 45, 365, 397
FaqI GGGAC 2 cut(s) 361, 530
FatI CATG 5 cut(s) 128, 338, 636, 684, 916
Fnu4HI GCNGC 2 cut(s) 336, 1245
Fsp4HI GCNGC 2 cut(s) 336, 1245
FspBI CTAG 1 cut(s) 489
GlaI GCGC 1 cut(s) 965
GluI GCNGC 2 cut(s) 336, 1245
GsuI CTGGAG 2 cut(s) 624, 1220
HaeIII GGCC 4 cut(s) 663, 837, 909, 1187
HapII CCGG 1 cut(s) 512
HhaI GCGC 1 cut(s) 966
Hin1II CATG 5 cut(s) 132, 342, 640, 688, 920
Hin6I GCGC 1 cut(s) 964
HinP1I GCGC 1 cut(s) 964
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HinfI GANTC 6 cut(s) 57, 214, 320, 508, 557, 716
HpaII CCGG 1 cut(s) 512
HphI GGTGA 1 cut(s) 668
Hpy166II GTNNAC 1 cut(s) 1084
Hpy188I TCNGA 3 cut(s) 219, 1231, 1289
Hpy188III TCNNGA 6 cut(s) 21, 572, 677, 713, 752, 1237
Hpy8I GTNNAC 1 cut(s) 1084
HpyAV CCTTC 3 cut(s) 102, 569, 633
HpyCH4III ACNGT 2 cut(s) 730, 761
HpyCH4IV ACGT 2 cut(s) 416, 1105
HpyCH4V TGCA 6 cut(s) 362, 473, 613, 1040, 1151, 1171
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 341, 498, 897, 906
HpyF3I CTNAG 2 cut(s) 704, 1052
HpySE526I ACGT 2 cut(s) 416, 1105
Hsp92II CATG 5 cut(s) 132, 342, 640, 688, 920
HspAI GCGC 1 cut(s) 964
Kzo9I GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
LmnI GCTCC 2 cut(s) 188, 625
Lsp1109I GCAGC 2 cut(s) 347, 1256
LweI GCATC 5 cut(s) 23, 482, 513, 795, 1049
MaeI CTAG 1 cut(s) 489
MaeII ACGT 2 cut(s) 416, 1105
MaeIII GTNAC 2 cut(s) 694, 1055
MalI GATC 7 cut(s) 122, 178, 294, 750, 1050, 1077, 1233
MboI GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
MflI RGATCY 2 cut(s) 292, 1231
MhlI GDGCHC 1 cut(s) 622
MlsI TGGCCA 1 cut(s) 1187
MluNI TGGCCA 1 cut(s) 1187
MlyI GAGTC 2 cut(s) 51, 502
Mox20I TGGCCA 1 cut(s) 1187
MroXI GAANNNNTTC 2 cut(s) 28, 597
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
Msp20I TGGCCA 1 cut(s) 1187
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 513, 603
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 5 cut(s) 144, 341, 498, 897, 906
NciI CCSGG 1 cut(s) 513
NdeII GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
NlaIII CATG 5 cut(s) 132, 342, 640, 688, 920
NlaIV GGNNCC 4 cut(s) 184, 294, 357, 1003
NmeAIII GCCGAG 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 694, 1055
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 481
PaeR7I CTCGAG 1 cut(s) 875
PceI AGGCCT 1 cut(s) 909
PciI ACATGT 1 cut(s) 684
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 116
PctI GAATGC 1 cut(s) 708
PdmI GAANNNNTTC 2 cut(s) 28, 597
PfeI GAWTC 4 cut(s) 214, 320, 557, 716
PfoI TCCNGGA 1 cut(s) 601
PkrI GCNGC 2 cut(s) 337, 1246
PleI GAGTC 2 cut(s) 51, 502
PpsI GAGTC 2 cut(s) 51, 502
Ppu21I YACGTR 1 cut(s) 417
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 4 cut(s) 184, 294, 357, 1003
PspPI GGNCC 1 cut(s) 355
PspPPI RGGWCCY 1 cut(s) 355
PspXI VCTCGAGB 1 cut(s) 875
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 2 cut(s) 292, 1231
RsaI GTAC 4 cut(s) 161, 445, 683, 881
RsaNI GTAC 4 cut(s) 160, 444, 682, 880
SaqAI TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
SatI GCNGC 2 cut(s) 336, 1245
Sau3AI GATC 7 cut(s) 120, 176, 292, 748, 1048, 1075, 1231
Sau96I GGNCC 1 cut(s) 355
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 2 cut(s) 51, 502
ScrFI CCNGG 2 cut(s) 513, 603
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 5 cut(s) 23, 482, 513, 795, 1049
SfcI CTRYAG 1 cut(s) 360
Sfr274I CTCGAG 1 cut(s) 875
SfuI TTCGAA 1 cut(s) 481
SinI GGWCC 1 cut(s) 355
SlaI CTCGAG 1 cut(s) 875
SmlI CTYRAG 2 cut(s) 875, 911
SmoI CTYRAG 2 cut(s) 875, 911
SnaBI TACGTA 1 cut(s) 417
SseBI AGGCCT 1 cut(s) 909
SsiI CCGC 1 cut(s) 492
SspMI CTAG 1 cut(s) 489
StuI AGGCCT 1 cut(s) 909
StyD4I CCNGG 2 cut(s) 511, 601
StyI CCWWGG 2 cut(s) 288, 1032
TaaI ACNGT 2 cut(s) 730, 761
TaiI ACGT 2 cut(s) 419, 1108
TaqI TCGA 8 cut(s) 119, 348, 481, 571, 645, 719, 747, 876
TatI WGTACW 3 cut(s) 159, 681, 879
TfiI GAWTC 4 cut(s) 214, 320, 557, 716
Tru1I TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
Tru9I TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
TscAI CASTG 3 cut(s) 655, 766, 1060
TseFI GTSAC 2 cut(s) 694, 1055
TseI GCWGC 2 cut(s) 335, 1244
Tsp45I GTSAC 2 cut(s) 694, 1055
TspDTI ATGAA 5 cut(s) 84, 435, 625, 660, 933
TspRI CASTG 3 cut(s) 655, 766, 1060
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 355
XapI RAATTY 4 cut(s) 203, 629, 1136, 1165
XceI RCATGY 1 cut(s) 688
XhoI CTCGAG 1 cut(s) 875
XmnI GAANNNNTTC 2 cut(s) 28, 597
XspI CTAG 1 cut(s) 489
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.