Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0005926

Physical Location & Seq

Genomic Coordinates Forward (+)

1576252 .. 1578312

Gene Structure 2061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G001910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGTTTTCTTGGGCATCTTCAAGAAGATCTTCAGGGGTTGTTTTGGGTGTTAATCAACGACTCGGCTCTCTCACATTGTTCAGTAAATCACAACCTTCATCATCGCCATCTTCGTCTTCTTCGATCAGCTCATCATGTGGTGGCAGTTTTGGTTGTAATAATGAGTACAATCACAAGAGTAGATCAGGCTCCTCCAATAAGGGTAGTAAATTTGATTTGATTCAGAGGTCAGTTTCTTCAGGGGTTCTAATTCTCGGGTCAAGCATAGGCTATTCTTATTGGTCTTCTTCTACCTTGGATCCAAACGCCTCTGTTTGTTTTGCTGATTCCAAAGAGGAGGCAGCATGGGCAATCGAGCAGGACCCTGCAGAACAATTCAAGTCCCTCTTTTCAAATACATTTAGGAGAAAAAAGTTCTTTACGTATGAAAAAAAGATAAGAGAGCGAAGTACCCCTGAAAAGATTTTCAATTACTTTGCATCAGTTCGAACCCCTAGCGGAGAGTGCTTTATGACTCCGGGGGACTTGATGCGAGCTGTTGTTCCTGTGTTTCCTCCCTATGATTCAAATCGGGTTCGAGAAGGCTCTTTAAGAGGGGAAAATGTTCCTGGAGAATTGCAATGTGCTCCTTCTAAATTCTTCATGCTTTTCGACACTGATGGTGATGGCCTCATTTCATTTCAAGAGTACATGTTTTTCGTCACTCTCCTCAGCATTCCTGAATCGAGTTTTACTGTGGCATTTAAAATGTTCGATCTTGACAACAGTGGAGACATTGATAAGGAAGAGTTTAACAGAGTTATGGCATTGATGCGAAATCACAACAATAAAATCAAGGACGGCCACAGGGTCAAAGTTGGTGTAGAAGAGGGAGGAATACTCGAGTACTTCTTCGGCAAAGACGGCAAAAAAGGCCTTAAGCATGAAAGATTTGTTCAGTTTTTCAGGGATTTACACGAGGAAATGTTGCGCTTGGAGTTTGCTCATTATGACTACAAATCAAAAGGAACCATAACAGCTAAAGATTTTGCCTTGTCCTTGGTTGCATCTGCTGATCTCAGTGACTTATACAAGTTGCTTGATCGTGTTGACGATATTAACAACGCCCCACGTCTTAACAACATACAAATTACATACAAGGAATTTAAAGATTTTGCAGAGCTACGCAAGAATTTGCAGTCTTTCTCTTTGGCCATTTTCAGCTACGGCGAGGTTAATGGGGTTTTGACAAAATCAGATCTCCAGAGAGCAGCTTCCAAAGTATGTGGCATCAATGTTACAGACAATGTGATGGACATAATCTTCCATGTATTCGATGCAAATGGTGATGGTGACCTAAGTGCGAACGAGTTTGTCAAAGTTTTACAGAACCGTGGAGGTGAACCCGTAGAGTCAGGCCTCAGGGGATTCCTAACTTGCTTCTTAAACTGTGCAACTAAAAGCTGCTCCTACGCTCAGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

487

Amino Acids

54.39

Weight (kDa)

6.68

Isoelectric Point (pI)

44.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_5	PF13202	247 - 268	2.6e-06	EF hand
EF-hand_8	PF13833	406 - 457	4.2e-08	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005926)

Species	Orthologous Gene IDs
malus_domestica	MD02G1018100.v1.1 MD15G1162000.v1.1
prunus_persica	Prupe.7G257300_v2.0.a1
pyrus_communis	pycom15g14560
rosa_chinensis	RchiOBHm_Chr2g0087011 RchiOBHm_Chr7g0219711
rosa_laevigata	RLG00000002277 RLG00000015822
rosa_multiflora	Rmu_sc0000902.1_g000013 Rmu_sc0000902.1_g000014 Rmu_sc0010698.1_g000002
rosa_roxburghii	Rroxscaffold_2G00153960 Rroxscaffold_3G00239910 Rroxscaffold_3G00239920 Rroxscaffold_3G00239930
rosa_rugosa	Rorug01G0471500 Rorug07G0186800
rosa_samantha	Rh2AG024300 Rh2BG024800 Rh2CG024900 Rh2DG024800 Rh7AG330900 Rh7BG320600 Rh7CG348200 Rh7DG326300
rosa_wichuraiana	Rw2G001910 Rw2G001960 Rw7G027850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	498
AclWI	GGATC	2 cut(s)	293, 306
AcoI	YGGCCR	2 cut(s)	841, 1191
AcsI	RAATTY	4 cut(s)	209, 635, 1142, 1171
AcuI	CTGAAG	2 cut(s)	15, 222
AfaI	GTAC	4 cut(s)	167, 451, 689, 887
AflII	CTTAAG	1 cut(s)	917
AflIII	ACRYGT	1 cut(s)	690
AgsI	TTSAA	6 cut(s)	21, 379, 393, 469, 567, 683
AjiI	CACGTC	1 cut(s)	1112
AjnI	CCWGG	1 cut(s)	607
AluBI	AGCT	7 cut(s)	129, 536, 1019, 1162, 1203, 1253, 1443
AluI	AGCT	7 cut(s)	129, 536, 1019, 1162, 1203, 1253, 1443
Alw21I	GWGCWC	1 cut(s)	628
Alw26I	GTCTC	1 cut(s)	765
AlwI	GGATC	2 cut(s)	293, 306
Ama87I	CYCGRG	2 cut(s)	254, 881
AoxI	GGCC	5 cut(s)	667, 841, 913, 1191, 1396
ApeKI	GCWGC	3 cut(s)	341, 1250, 1443
ApoI	RAATTY	4 cut(s)	209, 635, 1142, 1171
Asp700I	GAANNNNTTC	2 cut(s)	28, 603
AspLEI	GCGC	1 cut(s)	972
AspS9I	GGNCC	1 cut(s)	361
AsuC2I	CCSGG	1 cut(s)	519
AsuHPI	GGTGA	4 cut(s)	674, 1337, 1343, 1391
AsuII	TTCGAA	1 cut(s)	487
AvaI	CYCGRG	2 cut(s)	254, 881
AvaII	GGWCC	1 cut(s)	361
AxyI	CCTNAGG	1 cut(s)	1400
BalI	TGGCCA	1 cut(s)	1193
BamHI	GGATCC	1 cut(s)	298
BauI	CACGAG	1 cut(s)	956
BbsI	GAAGAC	2 cut(s)	108, 276
Bbv12I	GWGCWC	1 cut(s)	628
BbvCI	CCTCAGC	1 cut(s)	710
BbvI	GCAGC	3 cut(s)	353, 1262, 1430
BccI	CCATC	5 cut(s)	115, 653, 659, 1285, 1322
BceAI	ACGGC	3 cut(s)	856, 919, 1222
BciT130I	CCWGG	1 cut(s)	609
BcnI	CCSGG	1 cut(s)	519
BcoDI	GTCTC	1 cut(s)	765
BfaI	CTAG	1 cut(s)	495
BfmI	CTRYAG	1 cut(s)	366
BfrI	CTTAAG	1 cut(s)	917
BglII	AGATCT	2 cut(s)	26, 1237
BisI	GCNGC	3 cut(s)	342, 1251, 1444
BlsI	GCNGC	3 cut(s)	343, 1252, 1445
BmcAI	AGTACT	1 cut(s)	887
Bme1390I	CCNGG	2 cut(s)	519, 609
Bme18I	GGWCC	1 cut(s)	361
BmeT110I	CYCGRG	2 cut(s)	254, 881
BmgBI	CACGTC	1 cut(s)	1112
BmgT120I	GGNCC	1 cut(s)	361
BmiI	GGNNCC	4 cut(s)	190, 300, 363, 1009
BmrFI	CCNGG	2 cut(s)	519, 609
BmsI	GCATC	7 cut(s)	23, 488, 519, 801, 1055, 1278, 1306
BpiI	GAAGAC	2 cut(s)	108, 276
BplI	GAGNNNNNCTC	2 cut(s)	864, 896
BpmI	CTGGAG	2 cut(s)	630, 1226
Bpu10I	CCTNAGC	1 cut(s)	710
Bpu14I	TTCGAA	1 cut(s)	487
BpuMI	CCSGG	1 cut(s)	519
BsaAI	YACGTR	1 cut(s)	423
BsaBI	GATNNNNATC	1 cut(s)	567
BsaJI	CCNNGG	4 cut(s)	294, 518, 1038, 1372
Bse21I	CCTNAGG	1 cut(s)	1400
Bse3DI	GCAATG	1 cut(s)	626
Bse8I	GATNNNNATC	1 cut(s)	567
BseBI	CCWGG	1 cut(s)	609
BseDI	CCNNGG	4 cut(s)	294, 518, 1038, 1372
BseJI	GATNNNNATC	1 cut(s)	567
BseMI	GCAATG	1 cut(s)	626
BseMII	CTCAG	3 cut(s)	724, 1072, 1414
BseRI	GAGGAG	3 cut(s)	181, 350, 698
BseXI	GCAGC	3 cut(s)	353, 1262, 1430
BshFI	GGCC	5 cut(s)	669, 843, 915, 1193, 1398
BsiHKAI	GWGCWC	1 cut(s)	628
BsiHKCI	CYCGRG	2 cut(s)	254, 881
BsiSI	CCGG	1 cut(s)	518
BslFI	GGGAC	2 cut(s)	367, 536
BsmAI	GTCTC	1 cut(s)	765
BsmFI	GGGAC	2 cut(s)	367, 536
BsmI	GAATGC	1 cut(s)	714
BsnI	GGCC	5 cut(s)	669, 843, 915, 1193, 1398
BsoBI	CYCGRG	2 cut(s)	254, 881
Bsp119I	TTCGAA	1 cut(s)	487
Bsp1286I	GDGCHC	1 cut(s)	628
Bsp143I	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
BspACI	CCGC	1 cut(s)	498
BspANI	GGCC	5 cut(s)	669, 843, 915, 1193, 1398
BspCNI	CTCAG	3 cut(s)	723, 1071, 1413
BspLI	GGNNCC	4 cut(s)	190, 300, 363, 1009
BspMAI	CTGCAG	1 cut(s)	370
BspPI	GGATC	2 cut(s)	293, 306
BspT104I	TTCGAA	1 cut(s)	487
BspTI	CTTAAG	1 cut(s)	917
BsrDI	GCAATG	1 cut(s)	626
BssECI	CCNNGG	4 cut(s)	294, 518, 1038, 1372
BssMI	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
BssSI	CACGAG	1 cut(s)	956
BssT1I	CCWWGG	2 cut(s)	294, 1038
Bst2BI	CACGAG	1 cut(s)	956
Bst2UI	CCWGG	1 cut(s)	609
Bst4CI	ACNGT	4 cut(s)	736, 767, 1373, 1430
Bst6I	CTCTTC	2 cut(s)	780, 861
BstAFI	CTTAAG	1 cut(s)	917
BstBAI	YACGTR	1 cut(s)	423
BstBI	TTCGAA	1 cut(s)	487
BstC8I	GCNNGC	1 cut(s)	534
BstDEI	CTNAG	5 cut(s)	710, 1058, 1337, 1400, 1455
BstDSI	CCRYGG	1 cut(s)	1372
BstEII	GGTNACC	1 cut(s)	1331
BstHHI	GCGC	1 cut(s)	972
BstKTI	GATC	8 cut(s)	29, 126, 185, 301, 757, 1057, 1084, 1240
BstMAI	GTCTC	1 cut(s)	765
BstMBI	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
BstMWI	GCNNNNNNNGC	4 cut(s)	347, 504, 903, 912
BstNI	CCWGG	1 cut(s)	609
BstNSI	RCATGY	1 cut(s)	694
BstPI	GGTNACC	1 cut(s)	1331
BstSCI	CCNGG	2 cut(s)	517, 607
BstSFI	CTRYAG	1 cut(s)	366
BstSNI	TACGTA	1 cut(s)	423
BstV1I	GCAGC	3 cut(s)	353, 1262, 1430
BstV2I	GAAGAC	2 cut(s)	108, 276
BstX2I	RGATCY	3 cut(s)	26, 298, 1237
BstYI	RGATCY	3 cut(s)	26, 298, 1237
Bsu36I	CCTNAGG	1 cut(s)	1400
BsuRI	GGCC	5 cut(s)	669, 843, 915, 1193, 1398
BtgI	CCRYGG	1 cut(s)	1372
BtgZI	GCGATG	1 cut(s)	87
BtrI	CACGTC	1 cut(s)	1112
BtsIMutI	CAGTG	3 cut(s)	654, 772, 1066
Cac8I	GCNNGC	1 cut(s)	534
CfoI	GCGC	1 cut(s)	972
Cfr13I	GGNCC	1 cut(s)	361
Csp6I	GTAC	4 cut(s)	166, 450, 688, 886
CspCI	CAANNNNNGTGG	2 cut(s)	1246, 1281
CviAII	CATG	6 cut(s)	135, 345, 643, 691, 923, 1307
CviQI	GTAC	4 cut(s)	166, 450, 688, 886
DdeI	CTNAG	5 cut(s)	710, 1058, 1337, 1400, 1455
DpnI	GATC	8 cut(s)	28, 125, 184, 300, 756, 1056, 1083, 1239
DpnII	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
DraI	TTTAAA	2 cut(s)	745, 1147
EaeI	YGGCCR	2 cut(s)	841, 1191
Eam1104I	CTCTTC	2 cut(s)	780, 861
EarI	CTCTTC	2 cut(s)	780, 861
Eco105I	TACGTA	1 cut(s)	423
Eco130I	CCWWGG	2 cut(s)	294, 1038
Eco147I	AGGCCT	2 cut(s)	915, 1398
Eco47I	GGWCC	1 cut(s)	361
Eco57I	CTGAAG	2 cut(s)	15, 222
Eco81I	CCTNAGG	1 cut(s)	1400
Eco88I	CYCGRG	2 cut(s)	254, 881
Eco91I	GGTNACC	1 cut(s)	1331
EcoO109I	RGGNCCY	1 cut(s)	361
EcoO65I	GGTNACC	1 cut(s)	1331
EcoRII	CCWGG	1 cut(s)	607
EcoT14I	CCWWGG	2 cut(s)	294, 1038
ErhI	CCWWGG	2 cut(s)	294, 1038
FaeI	CATG	6 cut(s)	138, 348, 646, 694, 926, 1310
FalI	AAGNNNNNCTT	4 cut(s)	13, 45, 371, 403
FaqI	GGGAC	2 cut(s)	367, 536
FatI	CATG	6 cut(s)	134, 344, 642, 690, 922, 1306
Fnu4HI	GCNGC	3 cut(s)	342, 1251, 1444
Fsp4HI	GCNGC	3 cut(s)	342, 1251, 1444
FspBI	CTAG	1 cut(s)	495
GlaI	GCGC	1 cut(s)	971
GluI	GCNGC	3 cut(s)	342, 1251, 1444
GsuI	CTGGAG	2 cut(s)	630, 1226
HaeIII	GGCC	5 cut(s)	669, 843, 915, 1193, 1398
HapII	CCGG	1 cut(s)	518
HhaI	GCGC	1 cut(s)	972
Hin1II	CATG	6 cut(s)	138, 348, 646, 694, 926, 1310
Hin6I	GCGC	1 cut(s)	970
HinP1I	GCGC	1 cut(s)	970
HincII	GTYRAC	1 cut(s)	1090
HindII	GTYRAC	1 cut(s)	1090
HinfI	GANTC	8 cut(s)	60, 220, 326, 514, 563, 722, 1391, 1407
HpaII	CCGG	1 cut(s)	518
HphI	GGTGA	4 cut(s)	674, 1337, 1343, 1391
Hpy166II	GTNNAC	2 cut(s)	1090, 1382
Hpy188I	TCNGA	3 cut(s)	225, 1237, 1458
Hpy188III	TCNNGA	6 cut(s)	21, 578, 683, 719, 758, 1243
Hpy8I	GTNNAC	2 cut(s)	1090, 1382
HpyAV	CCTTC	3 cut(s)	105, 575, 639
HpyCH4III	ACNGT	4 cut(s)	736, 767, 1373, 1430
HpyCH4IV	ACGT	2 cut(s)	422, 1111
HpyCH4V	TGCA	8 cut(s)	368, 479, 619, 1046, 1157, 1177, 1319, 1433
HpyF10VI	GCNNNNNNNGC	4 cut(s)	347, 504, 903, 912
HpyF3I	CTNAG	5 cut(s)	710, 1058, 1337, 1400, 1455
HpySE526I	ACGT	2 cut(s)	422, 1111
Hsp92II	CATG	6 cut(s)	138, 348, 646, 694, 926, 1310
HspAI	GCGC	1 cut(s)	970
Kzo9I	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
LmnI	GCTCC	3 cut(s)	194, 631, 1451
Lsp1109I	GCAGC	3 cut(s)	353, 1262, 1430
LweI	GCATC	7 cut(s)	23, 488, 519, 801, 1055, 1278, 1306
MaeI	CTAG	1 cut(s)	495
MaeII	ACGT	2 cut(s)	422, 1111
MaeIII	GTNAC	4 cut(s)	700, 1061, 1276, 1331
MalI	GATC	8 cut(s)	28, 125, 184, 300, 756, 1056, 1083, 1239
MboI	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
MflI	RGATCY	3 cut(s)	26, 298, 1237
MhlI	GDGCHC	1 cut(s)	628
MlsI	TGGCCA	1 cut(s)	1193
MluCI	AATT	9 cut(s)	209, 249, 374, 469, 614, 635, 1128, 1142, 1171
MluNI	TGGCCA	1 cut(s)	1193
MlyI	GAGTC	3 cut(s)	54, 508, 1400
Mox20I	TGGCCA	1 cut(s)	1193
MroXI	GAANNNNTTC	2 cut(s)	28, 603
MscI	TGGCCA	1 cut(s)	1193
Msp20I	TGGCCA	1 cut(s)	1193
MspCI	CTTAAG	1 cut(s)	917
MspI	CCGG	1 cut(s)	518
MspR9I	CCNGG	2 cut(s)	519, 609
Mva1269I	GAATGC	1 cut(s)	714
MvaI	CCWGG	1 cut(s)	609
MwoI	GCNNNNNNNGC	4 cut(s)	347, 504, 903, 912
NciI	CCSGG	1 cut(s)	519
NdeII	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
NlaIII	CATG	6 cut(s)	138, 348, 646, 694, 926, 1310
NlaIV	GGNNCC	4 cut(s)	190, 300, 363, 1009
NmeAIII	GCCGAG	1 cut(s)	42
NmuCI	GTSAC	3 cut(s)	700, 1061, 1331
NspI	RCATGY	1 cut(s)	694
NspV	TTCGAA	1 cut(s)	487
PaeR7I	CTCGAG	1 cut(s)	881
PceI	AGGCCT	2 cut(s)	915, 1398
PciI	ACATGT	1 cut(s)	690
PcsI	WCGNNNNNNNCGW	2 cut(s)	110, 119
PctI	GAATGC	1 cut(s)	714
PdmI	GAANNNNTTC	2 cut(s)	28, 603
PfeI	GAWTC	5 cut(s)	220, 326, 563, 722, 1407
PfoI	TCCNGGA	1 cut(s)	607
PkrI	GCNGC	3 cut(s)	343, 1252, 1445
PleI	GAGTC	3 cut(s)	54, 508, 1399
PpsI	GAGTC	3 cut(s)	54, 508, 1399
Ppu21I	YACGTR	1 cut(s)	423
PpuMI	RGGWCCY	1 cut(s)	361
PscI	ACATGT	1 cut(s)	690
Psp5II	RGGWCCY	1 cut(s)	361
Psp6I	CCWGG	1 cut(s)	607
PspEI	GGTNACC	1 cut(s)	1331
PspGI	CCWGG	1 cut(s)	607
PspN4I	GGNNCC	4 cut(s)	190, 300, 363, 1009
PspPI	GGNCC	1 cut(s)	361
PspPPI	RGGWCCY	1 cut(s)	361
PspXI	VCTCGAGB	1 cut(s)	881
PstI	CTGCAG	1 cut(s)	370
PsuI	RGATCY	3 cut(s)	26, 298, 1237
RsaI	GTAC	4 cut(s)	167, 451, 689, 887
RsaNI	GTAC	4 cut(s)	166, 450, 688, 886
SatI	GCNGC	3 cut(s)	342, 1251, 1444
Sau3AI	GATC	8 cut(s)	26, 123, 182, 298, 754, 1054, 1081, 1237
Sau96I	GGNCC	1 cut(s)	361
ScaI	AGTACT	1 cut(s)	887
SchI	GAGTC	3 cut(s)	54, 508, 1400
ScrFI	CCNGG	2 cut(s)	519, 609
SduI	GDGCHC	1 cut(s)	628
SfaNI	GCATC	7 cut(s)	23, 488, 519, 801, 1055, 1278, 1306
SfcI	CTRYAG	1 cut(s)	366
Sfr274I	CTCGAG	1 cut(s)	881
SfuI	TTCGAA	1 cut(s)	487
SinI	GGWCC	1 cut(s)	361
SlaI	CTCGAG	1 cut(s)	881
SmlI	CTYRAG	2 cut(s)	881, 917
SmoI	CTYRAG	2 cut(s)	881, 917
SnaBI	TACGTA	1 cut(s)	423
Sse9I	AATT	9 cut(s)	209, 249, 374, 469, 614, 635, 1128, 1142, 1171
SseBI	AGGCCT	2 cut(s)	915, 1398
SsiI	CCGC	1 cut(s)	498
SspMI	CTAG	1 cut(s)	495
StuI	AGGCCT	2 cut(s)	915, 1398
StyD4I	CCNGG	2 cut(s)	517, 607
StyI	CCWWGG	2 cut(s)	294, 1038
TaaI	ACNGT	4 cut(s)	736, 767, 1373, 1430
TaiI	ACGT	2 cut(s)	425, 1114
TaqI	TCGA	9 cut(s)	122, 354, 487, 577, 651, 725, 753, 882, 1314
TasI	AATT	9 cut(s)	209, 249, 374, 469, 614, 635, 1128, 1142, 1171
TatI	WGTACW	3 cut(s)	165, 687, 885
TfiI	GAWTC	5 cut(s)	220, 326, 563, 722, 1407
TscAI	CASTG	3 cut(s)	661, 772, 1066
TseFI	GTSAC	3 cut(s)	700, 1061, 1331
TseI	GCWGC	3 cut(s)	341, 1250, 1443
Tsp45I	GTSAC	3 cut(s)	700, 1061, 1331
TspDTI	ATGAA	5 cut(s)	87, 441, 631, 666, 939
TspRI	CASTG	3 cut(s)	661, 772, 1066
Vha464I	CTTAAG	1 cut(s)	917
VpaK11BI	GGWCC	1 cut(s)	361
XapI	RAATTY	4 cut(s)	209, 635, 1142, 1171
XceI	RCATGY	1 cut(s)	694
XhoI	CTCGAG	1 cut(s)	881
XmnI	GAANNNNTTC	2 cut(s)	28, 603
XspI	CTAG	1 cut(s)	495
ZrmI	AGTACT	1 cut(s)	887

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.