Rh2BG024800

Calcium uptake protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005926
Physical Location & Seq
Forward (+)
1663580 .. 1666798
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG024800.1

Sequence Viewer

Length: 1404 bp
ATGTTTTCTTGGGCATCTTCAAGAAGATCTTCAGGGGTTGTTTTGGGTGTTCAACGACTCGGCTCTCGCACATTGTTCAGTAAATCACAACCCTCATCATCGCCATCTTCGTCTTCTTCGATCAGCCCATCATGTGGTGGCAGTTTTGGCTGTAATAGTGAGTACAATCACAAGAGTAGATCAGGTTCCTCCAATAAGGGTGGTAAATTTGATTTGATTCAGAGGTCAGTTTCTTCAGGGGTTCTAATTCTCGGGTCAAGCATAGGCTATTCTTATTGGTCTTCTTCTACCTTGGATCCAAACGCCTCTGTTTGTTTTGCTGATTCCAAAGAGGAGGCAGCATGGGCAATCGAGCAGGACCCAGAACAATTCAAGTCCCTCTTTTCAAATACATTTAGGAGAAAGAAGTTCTTTACGTATGAAAAAAAGATAAGAGAGCGAAGTACCCCTGAAAAGATTTTCAGTTACTTTGCATCAGTTCGAACCCCTAGCGGAGAGTGCTTTATGACTCCGGGGGACTTGATGCGAGCTGTTGTTCCTGTGTTTCCTCCCTATGATTCAAATCGGGTTCGAGAAGGCTCTTTAAGAGGGGAAAATGTTCCTGGAGAATTGCAATGTGCTCCTTCTAAATTCTTCATGCTTTTCGACACTGATGGTGATGGCCTCATTTCATTTCAAGAGTACATGTTTTTCGTCACTCTCCTCAGCATTCCTGAATCGAGTTTTACTGTGGCTTTTAAAATGTTCGATCTTGACAACAGTGGAGACATTGATAAGGAAGAGTTTAACAGAGTTATGGCATTGATGCGAAATCACAACAATAAAATCAAGGACGGCCACAGGGTCAAAGTTGGTGTAGAAGAGGGAGGAATACTCGAGTACTTCTTCGGCAAAGACGGCAAAAAAGGCCTTAAGCATGAAAGATTTGTTCAGTTTTTCAGGGATTTACACGAGGAAATGTTGCGCCTGGAGTTTGCTCATTATGACTACAAATCAAAAGGAACCATAACAGCTAAAGATTTTGCCTTGTCCTTGGTTGCATCTGCTGATCTCAGTGACTTATACAAGTTGCTTGATCGTGTTGACGATATTAACAACGCCCCACGTCTTAACAACATACAAATTACATACAAGGAATTTAAAGATTTTGCAGAGCTACGCAAGAATTTGCAGTCTTTCTCTTTGGCCATTTTCAGCTACGGCGAGGTTAATGGGGTTTTGACAAAATCAGATCTCCAGAGAGCAGCTTCCAAAGTACATTCTCACATACCAATCTATCTGTTCCTTCTGATTAGCTTCTTTCATGTGGATACTAACAATCAACTAATCGACACAGGTATGTGGCATCAATGTTACAGACAATGTGATGGACATAATCTTCCATGTATTCGATGCAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

467

Amino Acids

52.86

Weight (kDa)

8.57

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_5 PF13202 245 - 266 2.5e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 134
AciI CCGC 1 cut(s) 492
AclWI GGATC 2 cut(s) 290, 303
AcoI YGGCCR 2 cut(s) 835, 1185
AcsI RAATTY 4 cut(s) 206, 629, 1136, 1165
AcuI CTGAAG 2 cut(s) 15, 219
AfaI GTAC 5 cut(s) 164, 445, 683, 881, 1257
AfiI CCNNNNNNNGG 1 cut(s) 134
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 6 cut(s) 21, 53, 373, 387, 561, 677
AjiI CACGTC 1 cut(s) 1106
AjnI CCWGG 2 cut(s) 601, 966
AluBI AGCT 6 cut(s) 530, 1013, 1156, 1197, 1247, 1296
AluI AGCT 6 cut(s) 530, 1013, 1156, 1197, 1247, 1296
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 1 cut(s) 759
AlwI GGATC 2 cut(s) 290, 303
Ama87I CYCGRG 2 cut(s) 251, 875
AoxI GGCC 4 cut(s) 661, 835, 907, 1185
ApeKI GCWGC 2 cut(s) 338, 1244
ApoI RAATTY 4 cut(s) 206, 629, 1136, 1165
Asp700I GAANNNNTTC 2 cut(s) 28, 597
AspLEI GCGC 1 cut(s) 966
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 1 cut(s) 668
AsuII TTCGAA 1 cut(s) 481
AvaI CYCGRG 2 cut(s) 251, 875
AvaII GGWCC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 1187
BamHI GGATCC 1 cut(s) 295
BauI CACGAG 1 cut(s) 950
BbsI GAAGAC 2 cut(s) 105, 273
Bbv12I GWGCWC 1 cut(s) 622
BbvCI CCTCAGC 1 cut(s) 704
BbvI GCAGC 2 cut(s) 350, 1256
BccI CCATC 5 cut(s) 112, 136, 647, 653, 1361
BceAI ACGGC 3 cut(s) 850, 913, 1216
BciT130I CCWGG 2 cut(s) 603, 968
BciVI GTATCC 1 cut(s) 1303
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 759
BfaI CTAG 1 cut(s) 489
BfrI CTTAAG 1 cut(s) 911
BfuI GTATCC 1 cut(s) 1303
BglII AGATCT 2 cut(s) 26, 1231
BisI GCNGC 2 cut(s) 339, 1245
BlsI GCNGC 2 cut(s) 340, 1246
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 3 cut(s) 513, 603, 968
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 251, 875
BmgBI CACGTC 1 cut(s) 1106
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 4 cut(s) 187, 297, 360, 1003
BmrFI CCNGG 3 cut(s) 513, 603, 968
BmsI GCATC 7 cut(s) 23, 482, 513, 795, 1049, 1354, 1382
BpiI GAAGAC 2 cut(s) 105, 273
BplI GAGNNNNNCTC 2 cut(s) 858, 890
BpmI CTGGAG 3 cut(s) 624, 989, 1220
Bpu10I CCTNAGC 1 cut(s) 704
Bpu14I TTCGAA 1 cut(s) 481
BpuMI CCSGG 1 cut(s) 513
BsaAI YACGTR 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 291, 512, 1032
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse3DI GCAATG 1 cut(s) 620
Bse8I GATNNNNATC 1 cut(s) 561
BseBI CCWGG 2 cut(s) 603, 968
BseDI CCNNGG 3 cut(s) 291, 512, 1032
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 2 cut(s) 718, 1066
BseRI GAGGAG 2 cut(s) 347, 692
BseXI GCAGC 2 cut(s) 350, 1256
BshFI GGCC 4 cut(s) 663, 837, 909, 1187
BsiHKAI GWGCWC 1 cut(s) 622
BsiHKCI CYCGRG 2 cut(s) 251, 875
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 2 cut(s) 361, 530
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 759
BsmFI GGGAC 2 cut(s) 361, 530
BsmI GAATGC 1 cut(s) 708
BsnI GGCC 4 cut(s) 663, 837, 909, 1187
BsoBI CYCGRG 2 cut(s) 251, 875
Bsp119I TTCGAA 1 cut(s) 481
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
BspACI CCGC 1 cut(s) 492
BspANI GGCC 4 cut(s) 663, 837, 909, 1187
BspCNI CTCAG 2 cut(s) 717, 1065
BspLI GGNNCC 4 cut(s) 187, 297, 360, 1003
BspPI GGATC 2 cut(s) 290, 303
BspT104I TTCGAA 1 cut(s) 481
BspTI CTTAAG 1 cut(s) 911
BsrDI GCAATG 1 cut(s) 620
BssECI CCNNGG 3 cut(s) 291, 512, 1032
BssMI GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
BssSI CACGAG 1 cut(s) 950
BssT1I CCWWGG 2 cut(s) 291, 1032
Bst2BI CACGAG 1 cut(s) 950
Bst2UI CCWGG 2 cut(s) 603, 968
Bst4CI ACNGT 3 cut(s) 730, 761, 1401
Bst6I CTCTTC 2 cut(s) 774, 855
BstAFI CTTAAG 1 cut(s) 911
BstBAI YACGTR 1 cut(s) 417
BstBI TTCGAA 1 cut(s) 481
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 704, 1052
BstHHI GCGC 1 cut(s) 966
BstKTI GATC 8 cut(s) 29, 123, 182, 298, 751, 1051, 1078, 1234
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
BstMWI GCNNNNNNNGC 5 cut(s) 147, 344, 498, 897, 906
BstNI CCWGG 2 cut(s) 603, 968
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 3 cut(s) 511, 601, 966
BstSNI TACGTA 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 350, 1256
BstV2I GAAGAC 2 cut(s) 105, 273
BstX2I RGATCY 3 cut(s) 26, 295, 1231
BstYI RGATCY 3 cut(s) 26, 295, 1231
BsuI GTATCC 1 cut(s) 1303
BsuRI GGCC 4 cut(s) 663, 837, 909, 1187
BtgZI GCGATG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 1106
BtsIMutI CAGTG 3 cut(s) 648, 766, 1060
Cac8I GCNNGC 1 cut(s) 528
CfoI GCGC 1 cut(s) 966
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 5 cut(s) 163, 444, 682, 880, 1256
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 7 cut(s) 132, 342, 637, 685, 917, 1304, 1383
CviQI GTAC 5 cut(s) 163, 444, 682, 880, 1256
DdeI CTNAG 2 cut(s) 704, 1052
DpnI GATC 8 cut(s) 28, 122, 181, 297, 750, 1050, 1077, 1233
DpnII GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
DraI TTTAAA 2 cut(s) 739, 1141
EaeI YGGCCR 2 cut(s) 835, 1185
Eam1104I CTCTTC 2 cut(s) 774, 855
EarI CTCTTC 2 cut(s) 774, 855
Eco105I TACGTA 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 291, 1032
Eco147I AGGCCT 1 cut(s) 909
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 2 cut(s) 15, 219
Eco88I CYCGRG 2 cut(s) 251, 875
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 601, 966
EcoT14I CCWWGG 2 cut(s) 291, 1032
ErhI CCWWGG 2 cut(s) 291, 1032
FaeI CATG 7 cut(s) 135, 345, 640, 688, 920, 1307, 1386
FalI AAGNNNNNCTT 6 cut(s) 13, 45, 365, 397, 395, 427
FaqI GGGAC 2 cut(s) 361, 530
FatI CATG 7 cut(s) 131, 341, 636, 684, 916, 1303, 1382
Fnu4HI GCNGC 2 cut(s) 339, 1245
Fsp4HI GCNGC 2 cut(s) 339, 1245
FspBI CTAG 1 cut(s) 489
GlaI GCGC 1 cut(s) 965
GluI GCNGC 2 cut(s) 339, 1245
GsuI CTGGAG 3 cut(s) 624, 989, 1220
HaeIII GGCC 4 cut(s) 663, 837, 909, 1187
HapII CCGG 1 cut(s) 512
HhaI GCGC 1 cut(s) 966
Hin1II CATG 7 cut(s) 135, 345, 640, 688, 920, 1307, 1386
Hin6I GCGC 1 cut(s) 964
HinP1I GCGC 1 cut(s) 964
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HinfI GANTC 6 cut(s) 57, 217, 323, 508, 557, 716
HpaII CCGG 1 cut(s) 512
HphI GGTGA 1 cut(s) 668
Hpy166II GTNNAC 1 cut(s) 1084
Hpy188I TCNGA 3 cut(s) 222, 1231, 1290
Hpy188III TCNNGA 6 cut(s) 21, 572, 677, 713, 752, 1237
Hpy8I GTNNAC 1 cut(s) 1084
HpyAV CCTTC 3 cut(s) 569, 633, 1295
HpyCH4III ACNGT 3 cut(s) 730, 761, 1401
HpyCH4IV ACGT 2 cut(s) 416, 1105
HpyCH4V TGCA 6 cut(s) 473, 613, 1040, 1151, 1171, 1395
HpyF10VI GCNNNNNNNGC 5 cut(s) 147, 344, 498, 897, 906
HpyF3I CTNAG 2 cut(s) 704, 1052
HpySE526I ACGT 2 cut(s) 416, 1105
Hsp92II CATG 7 cut(s) 135, 345, 640, 688, 920, 1307, 1386
HspAI GCGC 1 cut(s) 964
Kzo9I GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 2 cut(s) 350, 1256
LweI GCATC 7 cut(s) 23, 482, 513, 795, 1049, 1354, 1382
MaeI CTAG 1 cut(s) 489
MaeII ACGT 2 cut(s) 416, 1105
MaeIII GTNAC 4 cut(s) 464, 694, 1055, 1352
MalI GATC 8 cut(s) 28, 122, 181, 297, 750, 1050, 1077, 1233
MboI GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
MflI RGATCY 3 cut(s) 26, 295, 1231
MhlI GDGCHC 1 cut(s) 622
MlsI TGGCCA 1 cut(s) 1187
MluCI AATT 8 cut(s) 206, 246, 368, 608, 629, 1122, 1136, 1165
MluNI TGGCCA 1 cut(s) 1187
MlyI GAGTC 2 cut(s) 51, 502
Mox20I TGGCCA 1 cut(s) 1187
MroXI GAANNNNTTC 2 cut(s) 28, 597
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
MslI CAYNNNNRTG 1 cut(s) 1337
Msp20I TGGCCA 1 cut(s) 1187
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 3 cut(s) 513, 603, 968
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 2 cut(s) 603, 968
MwoI GCNNNNNNNGC 5 cut(s) 147, 344, 498, 897, 906
NciI CCSGG 1 cut(s) 513
NdeII GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
NlaIII CATG 7 cut(s) 135, 345, 640, 688, 920, 1307, 1386
NlaIV GGNNCC 4 cut(s) 187, 297, 360, 1003
NmeAIII GCCGAG 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 694, 1055
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 481
PaeR7I CTCGAG 1 cut(s) 875
PceI AGGCCT 1 cut(s) 909
PciI ACATGT 1 cut(s) 684
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 116
PctI GAATGC 1 cut(s) 708
PdmI GAANNNNTTC 2 cut(s) 28, 597
PfeI GAWTC 4 cut(s) 217, 323, 557, 716
PflMI CCANNNNNTGG 1 cut(s) 134
PfoI TCCNGGA 1 cut(s) 601
PkrI GCNGC 2 cut(s) 340, 1246
PleI GAGTC 2 cut(s) 51, 502
PpsI GAGTC 2 cut(s) 51, 502
Ppu21I YACGTR 1 cut(s) 417
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 601, 966
PspGI CCWGG 2 cut(s) 601, 966
PspN4I GGNNCC 4 cut(s) 187, 297, 360, 1003
PspPI GGNCC 1 cut(s) 358
PspPPI RGGWCCY 1 cut(s) 358
PspXI VCTCGAGB 1 cut(s) 875
PsrI GAACNNNNNNTAC 2 cut(s) 169, 201
PsuI RGATCY 3 cut(s) 26, 295, 1231
RsaI GTAC 5 cut(s) 164, 445, 683, 881, 1257
RsaNI GTAC 5 cut(s) 163, 444, 682, 880, 1256
RseI CAYNNNNRTG 1 cut(s) 1337
SaqAI TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
SatI GCNGC 2 cut(s) 339, 1245
Sau3AI GATC 8 cut(s) 26, 120, 179, 295, 748, 1048, 1075, 1231
Sau96I GGNCC 1 cut(s) 358
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 2 cut(s) 51, 502
ScrFI CCNGG 3 cut(s) 513, 603, 968
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 7 cut(s) 23, 482, 513, 795, 1049, 1354, 1382
Sfr274I CTCGAG 1 cut(s) 875
SfuI TTCGAA 1 cut(s) 481
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 1 cut(s) 875
SmiMI CAYNNNNRTG 1 cut(s) 1337
SmlI CTYRAG 2 cut(s) 875, 911
SmoI CTYRAG 2 cut(s) 875, 911
SnaBI TACGTA 1 cut(s) 417
Sse9I AATT 8 cut(s) 206, 246, 368, 608, 629, 1122, 1136, 1165
SseBI AGGCCT 1 cut(s) 909
SsiI CCGC 1 cut(s) 492
SspMI CTAG 1 cut(s) 489
StuI AGGCCT 1 cut(s) 909
StyD4I CCNGG 3 cut(s) 511, 601, 966
StyI CCWWGG 2 cut(s) 291, 1032
TaaI ACNGT 3 cut(s) 730, 761, 1401
TaiI ACGT 2 cut(s) 419, 1108
TasI AATT 8 cut(s) 206, 246, 368, 608, 629, 1122, 1136, 1165
TatI WGTACW 4 cut(s) 162, 681, 879, 1255
TfiI GAWTC 4 cut(s) 217, 323, 557, 716
Tru1I TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
Tru9I TTAA 8 cut(s) 584, 738, 786, 912, 1092, 1110, 1140, 1209
TscAI CASTG 3 cut(s) 655, 766, 1060
TseFI GTSAC 2 cut(s) 694, 1055
TseI GCWGC 2 cut(s) 338, 1244
Tsp45I GTSAC 2 cut(s) 694, 1055
TspDTI ATGAA 5 cut(s) 435, 625, 660, 933, 1292
TspRI CASTG 3 cut(s) 655, 766, 1060
Van91I CCANNNNNTGG 1 cut(s) 134
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 4 cut(s) 206, 629, 1136, 1165
XceI RCATGY 1 cut(s) 688
XhoI CTCGAG 1 cut(s) 875
XmnI GAANNNNTTC 2 cut(s) 28, 597
XspI CTAG 1 cut(s) 489
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.