Rroxscaffold_3G00239920

Calcium uptake protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005926
Physical Location & Seq
Reverse (-)
31157538 .. 31157947
410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239920.1

Sequence Viewer

Length: 297 bp
ATGAATATTAGGTACATGTTTTTCGTCACTCTCCTCAGCATTCCTGAATCGAGTTTTACTGTGGCCTTTAAAATGTTCGATCTTGACAACAGTGGAGACATTGATAGGGAAGAGTTTAACAGAGTTATGGCATTGATGCGAAATCACAACAATAAAAACAAGGACGGCCACAGGGTCAAAGTTGGTGTACAAGAGGGAGGCATACTCGAGTACTTCTTCGGCAAAGACGGCAAAAAAGGCCTTAAGCATGAAAGATTTGTTCAGTTTTTCAGGGATTTACACGAGGAAGTACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

98

Amino Acids

11.62

Weight (kDa)

8.06

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 21 - 44 9.3e-06 EF-hand domain
EF-hand_5 PF13202 22 - 43 5.8e-07 EF hand
EF-hand_1 PF00036 22 - 45 1.4e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 166
AfaI GTAC 4 cut(s) 14, 189, 212, 291
AflII CTTAAG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 90
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 3 cut(s) 63, 166, 238
AvaI CYCGRG 1 cut(s) 206
BauI CACGAG 1 cut(s) 281
BbvCI CCTCAGC 1 cut(s) 35
BceAI ACGGC 2 cut(s) 181, 244
BcoDI GTCTC 1 cut(s) 90
BfrI CTTAAG 1 cut(s) 242
BmcAI AGTACT 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 206
BmsI GCATC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 189, 221
Bpu10I CCTNAGC 1 cut(s) 35
BseMII CTCAG 1 cut(s) 49
BseRI GAGGAG 1 cut(s) 23
BshFI GGCC 3 cut(s) 65, 168, 240
BsiHKCI CYCGRG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 3 cut(s) 65, 168, 240
BsoBI CYCGRG 1 cut(s) 206
Bsp1407I TGTACA 1 cut(s) 187
Bsp143I GATC 1 cut(s) 79
BspANI GGCC 3 cut(s) 65, 168, 240
BspCNI CTCAG 1 cut(s) 48
BspTI CTTAAG 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 187
BssMI GATC 1 cut(s) 79
BssSI CACGAG 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 61, 92
Bst6I CTCTTC 1 cut(s) 105
BstAFI CTTAAG 1 cut(s) 242
BstAUI TGTACA 1 cut(s) 187
BstDEI CTNAG 1 cut(s) 35
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 2 cut(s) 228, 237
BstNSI RCATGY 1 cut(s) 19
BsuRI GGCC 3 cut(s) 65, 168, 240
BtsIMutI CAGTG 1 cut(s) 97
Csp6I GTAC 4 cut(s) 13, 188, 211, 290
CviAII CATG 2 cut(s) 16, 248
CviJI RGCY 3 cut(s) 65, 168, 240
CviKI_1 RGCY 3 cut(s) 65, 168, 240
CviQI GTAC 4 cut(s) 13, 188, 211, 290
DdeI CTNAG 1 cut(s) 35
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
DraI TTTAAA 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco147I AGGCCT 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 206
FaeI CATG 2 cut(s) 19, 251
FaiI YATR 4 cut(s) 17, 128, 203, 249
FatI CATG 2 cut(s) 15, 247
HaeIII GGCC 3 cut(s) 65, 168, 240
Hin1II CATG 2 cut(s) 19, 251
HinfI GANTC 1 cut(s) 47
Hpy166II GTNNAC 1 cut(s) 188
Hpy188III TCNNGA 2 cut(s) 44, 83
Hpy8I GTNNAC 1 cut(s) 188
HpyCH4III ACNGT 2 cut(s) 61, 92
HpyCH4IV ACGT 1 cut(s) 292
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 237
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 2 cut(s) 19, 251
Kzo9I GATC 1 cut(s) 79
LpnPI CCDG 3 cut(s) 57, 157, 256
LweI GCATC 1 cut(s) 126
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 25
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 2 cut(s) 122, 208
MnlI CCTC 4 cut(s) 44, 187, 191, 277
MseI TTAA 4 cut(s) 69, 117, 243, 295
MspCI CTTAAG 1 cut(s) 242
Mva1269I GAATGC 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 228, 237
NdeII GATC 1 cut(s) 79
NlaIII CATG 2 cut(s) 19, 251
NmuCI GTSAC 1 cut(s) 25
NspI RCATGY 1 cut(s) 19
PaeR7I CTCGAG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 240
PciI ACATGT 1 cut(s) 15
PctI GAATGC 1 cut(s) 39
PfeI GAWTC 1 cut(s) 47
PscI ACATGT 1 cut(s) 15
PspXI VCTCGAGB 1 cut(s) 206
RsaI GTAC 4 cut(s) 14, 189, 212, 291
RsaNI GTAC 4 cut(s) 13, 188, 211, 290
SaqAI TTAA 4 cut(s) 69, 117, 243, 295
Sau3AI GATC 1 cut(s) 79
ScaI AGTACT 1 cut(s) 212
SetI ASST 2 cut(s) 14, 295
SfaNI GCATC 1 cut(s) 126
Sfr274I CTCGAG 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 2 cut(s) 206, 242
SmoI CTYRAG 2 cut(s) 206, 242
SseBI AGGCCT 1 cut(s) 240
SspI AATATT 1 cut(s) 7
StuI AGGCCT 1 cut(s) 240
TaaI ACNGT 2 cut(s) 61, 92
TaiI ACGT 1 cut(s) 295
TaqI TCGA 3 cut(s) 50, 78, 207
TatI WGTACW 2 cut(s) 187, 210
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 4 cut(s) 69, 117, 243, 295
Tru9I TTAA 4 cut(s) 69, 117, 243, 295
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 1 cut(s) 25
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 17, 264
TspRI CASTG 1 cut(s) 97
Vha464I CTTAAG 1 cut(s) 242
XceI RCATGY 1 cut(s) 19
XhoI CTCGAG 1 cut(s) 206
ZrmI AGTACT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.