Rroxscaffold_3G00239930

Calcium uptake protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005926
Physical Location & Seq
Reverse (-)
31158071 .. 31159051
981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239930.1

Sequence Viewer

Length: 621 bp
ATGTTTTCTTGGGCATCTTCAAGAAGATCCTCAGGGGTTGTTTTGGGTGTTCAACGACTCGGCTCTCGCACATTGTTCAGTAAATCACAACCCTTATCATCGCCATCTTCGTCTTCTTCGATCAGCTCATCATGTGGTGGCACTTTTGGCTGTAACAATGAGTACAATTACAAGAGTAGATCAGGCTCCTCTAATAAGCGTAGTACATTTAATTTGATTCAGAGGTCAGTTTCTTCAGGGGTTCTAATTCTCGGGTCAAGCATAGGCTATTCTTATTGGTCTTCTGCTTCCTTGGATCCAAATGCCTCTGTTTGTTTTGCTGATTCCAAAGAGGAGGCAGCATGGGCAATCGAGCAGGACCCAGAACAGTTCAAGTCCCTCTTTTCAAGTACAATTTTCAATTACTTTGCATCAGTTCGAACCCCTAGCGGAGAGTGCTTTATGACTCCGGGGGACTTGATGCGAGCTGTTGTTCCTGTGTTTCCTCCCTATGATTCAAATCGGGTTCGAGAAGGCTCTTTAAGAGGGGAAAATGTTCCTGGAGAATTGCAATGTGCTCCTTCTAAATTCTTCATGCTTTTCGACACTGATGGTGATGGCCTCATTTCATTTCAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

206

Amino Acids

22.35

Weight (kDa)

6.34

Isoelectric Point (pI)

65.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 429
AclWI GGATC 3 cut(s) 21, 290, 303
AcsI RAATTY 1 cut(s) 566
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 3 cut(s) 164, 205, 391
AgsI TTSAA 7 cut(s) 21, 53, 373, 387, 400, 498, 614
AjnI CCWGG 1 cut(s) 538
AluBI AGCT 2 cut(s) 126, 467
AluI AGCT 2 cut(s) 126, 467
Alw21I GWGCWC 1 cut(s) 559
AlwI GGATC 3 cut(s) 21, 290, 303
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 1 cut(s) 598
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 566
Asp700I GAANNNNTTC 1 cut(s) 534
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 605
AsuII TTCGAA 1 cut(s) 418
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 31
BamHI GGATCC 1 cut(s) 295
BbsI GAAGAC 2 cut(s) 105, 273
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 1 cut(s) 350
BccI CCATC 3 cut(s) 112, 584, 590
BciT130I CCWGG 1 cut(s) 540
BcnI CCSGG 1 cut(s) 450
BfaI CTAG 1 cut(s) 426
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 2 cut(s) 450, 540
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 3 cut(s) 187, 297, 360
BmrFI CCNGG 2 cut(s) 450, 540
BmsI GCATC 3 cut(s) 23, 419, 450
BpiI GAAGAC 2 cut(s) 105, 273
BpmI CTGGAG 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 418
BpuMI CCSGG 1 cut(s) 450
BsaBI GATNNNNATC 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 291, 449
Bse21I CCTNAGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 291, 449
BseJI GATNNNNATC 1 cut(s) 498
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 1 cut(s) 45
BseRI GAGGAG 2 cut(s) 178, 347
BseXI GCAGC 1 cut(s) 350
BshFI GGCC 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 559
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 449
BslFI GGGAC 2 cut(s) 361, 467
BsmFI GGGAC 2 cut(s) 361, 467
BsnI GGCC 1 cut(s) 600
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 559
Bsp143I GATC 4 cut(s) 26, 120, 179, 295
BspACI CCGC 1 cut(s) 429
BspANI GGCC 1 cut(s) 600
BspCNI CTCAG 1 cut(s) 44
BspLI GGNNCC 3 cut(s) 187, 297, 360
BspPI GGATC 3 cut(s) 21, 290, 303
BspT104I TTCGAA 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 557
BssECI CCNNGG 2 cut(s) 291, 449
BssMI GATC 4 cut(s) 26, 120, 179, 295
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 1 cut(s) 369
BstBI TTCGAA 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 1 cut(s) 31
BstKTI GATC 4 cut(s) 29, 123, 182, 298
BstMBI GATC 4 cut(s) 26, 120, 179, 295
BstMWI GCNNNNNNNGC 3 cut(s) 147, 344, 435
BstNI CCWGG 1 cut(s) 540
BstSCI CCNGG 2 cut(s) 448, 538
BstV1I GCAGC 1 cut(s) 350
BstV2I GAAGAC 2 cut(s) 105, 273
BstX2I RGATCY 2 cut(s) 26, 295
BstYI RGATCY 2 cut(s) 26, 295
Bsu36I CCTNAGG 1 cut(s) 31
BsuRI GGCC 1 cut(s) 600
BtgZI GCGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 585
Cac8I GCNNGC 1 cut(s) 465
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 3 cut(s) 163, 204, 390
CviAII CATG 3 cut(s) 132, 342, 574
CviJI RGCY 8 cut(s) 63, 126, 150, 186, 267, 467, 516, 600
CviKI_1 RGCY 8 cut(s) 63, 126, 150, 186, 267, 467, 516, 600
CviQI GTAC 3 cut(s) 163, 204, 390
DdeI CTNAG 1 cut(s) 31
DpnI GATC 4 cut(s) 28, 122, 181, 297
DpnII GATC 4 cut(s) 26, 120, 179, 295
Eco130I CCWWGG 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 3 cut(s) 135, 345, 577
FaiI YATR 6 cut(s) 133, 263, 343, 443, 492, 575
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 2 cut(s) 361, 467
FatI CATG 3 cut(s) 131, 341, 573
Fnu4HI GCNGC 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 1 cut(s) 426
GluI GCNGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 1 cut(s) 600
HapII CCGG 1 cut(s) 449
Hin1II CATG 3 cut(s) 135, 345, 577
HinfI GANTC 5 cut(s) 57, 217, 323, 445, 494
HpaII CCGG 1 cut(s) 449
HphI GGTGA 1 cut(s) 605
Hpy188I TCNGA 1 cut(s) 222
Hpy188III TCNNGA 3 cut(s) 21, 509, 614
HpyAV CCTTC 2 cut(s) 506, 570
HpyCH4III ACNGT 1 cut(s) 369
HpyCH4V TGCA 2 cut(s) 410, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 344, 435
HpyF3I CTNAG 1 cut(s) 31
Hsp92II CATG 3 cut(s) 135, 345, 577
Kzo9I GATC 4 cut(s) 26, 120, 179, 295
LmnI GCTCC 2 cut(s) 191, 562
LpnPI CCDG 9 cut(s) 18, 168, 222, 341, 375, 462, 489, 525, 552
Lsp1109I GCAGC 1 cut(s) 350
LweI GCATC 3 cut(s) 23, 419, 450
MaeI CTAG 1 cut(s) 426
MaeIII GTNAC 1 cut(s) 152
MalI GATC 4 cut(s) 28, 122, 181, 297
MboI GATC 4 cut(s) 26, 120, 179, 295
MboII GAAGA 8 cut(s) 9, 36, 99, 105, 108, 225, 273, 562
MflI RGATCY 2 cut(s) 26, 295
MhlI GDGCHC 1 cut(s) 559
MluCI AATT 7 cut(s) 166, 211, 246, 393, 400, 545, 566
MlyI GAGTC 2 cut(s) 51, 439
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 2 cut(s) 210, 521
MspI CCGG 1 cut(s) 449
MspR9I CCNGG 2 cut(s) 450, 540
MvaI CCWGG 1 cut(s) 540
MwoI GCNNNNNNNGC 3 cut(s) 147, 344, 435
NciI CCSGG 1 cut(s) 450
NdeII GATC 4 cut(s) 26, 120, 179, 295
NlaIII CATG 3 cut(s) 135, 345, 577
NlaIV GGNNCC 3 cut(s) 187, 297, 360
NmeAIII GCCGAG 1 cut(s) 39
NspV TTCGAA 1 cut(s) 418
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 116
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 3 cut(s) 217, 323, 494
PfoI TCCNGGA 1 cut(s) 538
PkrI GCNGC 1 cut(s) 340
PleI GAGTC 2 cut(s) 51, 439
PpsI GAGTC 2 cut(s) 51, 439
PpuMI RGGWCCY 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 538
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 3 cut(s) 187, 297, 360
PspPI GGNCC 1 cut(s) 358
PspPPI RGGWCCY 1 cut(s) 358
PsuI RGATCY 2 cut(s) 26, 295
RsaI GTAC 3 cut(s) 164, 205, 391
RsaNI GTAC 3 cut(s) 163, 204, 390
SaqAI TTAA 2 cut(s) 210, 521
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 4 cut(s) 26, 120, 179, 295
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 2 cut(s) 51, 439
ScrFI CCNGG 2 cut(s) 450, 540
SduI GDGCHC 1 cut(s) 559
SetI ASST 3 cut(s) 128, 227, 469
SfaNI GCATC 3 cut(s) 23, 419, 450
SfuI TTCGAA 1 cut(s) 418
SinI GGWCC 1 cut(s) 358
Sse9I AATT 7 cut(s) 166, 211, 246, 393, 400, 545, 566
SsiI CCGC 1 cut(s) 429
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 2 cut(s) 448, 538
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 369
TaqI TCGA 5 cut(s) 119, 351, 418, 508, 582
TasI AATT 7 cut(s) 166, 211, 246, 393, 400, 545, 566
TatI WGTACW 3 cut(s) 162, 203, 389
TfiI GAWTC 3 cut(s) 217, 323, 494
Tru1I TTAA 2 cut(s) 210, 521
Tru9I TTAA 2 cut(s) 210, 521
TscAI CASTG 1 cut(s) 592
TseI GCWGC 1 cut(s) 338
TspDTI ATGAA 2 cut(s) 562, 597
TspRI CASTG 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.