MD02G1074700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006945
Physical Location & Seq
Reverse (-)
5975190 .. 5975735
546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1074700.v1.1.491

Sequence Viewer

Length: 546 bp
ATGCAACAAGAAGGTGTAAAGCCAAACGAGTTCAGCATTGCTGGCTGTTTGAGTGCTTGCTCCCGTACAGCTGTGCTGGAAAATGGGCGGCAGCTCCACTTAATGGTAATTAAGAGTGGGCATCTGGAAGATTTGTTTGTTACTGGTGCACTTGTTGATATGTACGCAAAATGTGGGTGCATAAGTGATGCTGAGGATATCTTTGAGGGTTTGCTTTCACGAGATACAGTGGCATGGAATATCATGGTATGCGGATATTCCCAATATGGGCAAGGAGAGAAGGCTCTTGAAGCGTTTTCGACAATGTTGGATGAAGGCGCCATACCAAATGAGATCACCTTCATTGGTGTTCTTTCTGCATGCAGCCACCTGGGTCTAGTCAAAGAAGGGAAAAGGTACTTTGATTCTCTGACCAATGTTGTCGGGATCACTTCTACAATTGAGCATTATGCTTGTATGGTTGATATTCTCGGCAGGGCTGGAAAGTTTAATGAGATTGAAAGCTTCATCAAGACAAAGGAACTAACACCATATGCCATTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 map00190 map01100 map04714
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

182

Amino Acids

19.75

Weight (kDa)

4.86

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 122 1.3e-10 PPR repeat family
PPR PF01535 77 - 106 9.7e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AccB7I CCANNNNNTGG 1 cut(s) 103
AciI CCGC 2 cut(s) 88, 252
AclWI GGATC 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 3 cut(s) 67, 164, 398
AfiI CCNNNNNNNGG 2 cut(s) 103, 267
AgsI TTSAA 2 cut(s) 290, 500
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 3 cut(s) 71, 94, 504
AluI AGCT 3 cut(s) 71, 94, 504
Alw21I GWGCWC 1 cut(s) 151
Alw44I GTGCAC 1 cut(s) 147
AlwI GGATC 1 cut(s) 434
ApaLI GTGCAC 1 cut(s) 147
ApeKI GCWGC 2 cut(s) 91, 363
AspLEI GCGC 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 328
BaeGI GKGCMC 1 cut(s) 151
BanI GGYRCC 1 cut(s) 317
BarI GAAGNNNNNNTAC 2 cut(s) 306, 338
BauI CACGAG 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 151
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 103, 375
BciT130I CCWGG 1 cut(s) 371
BfaI CTAG 1 cut(s) 377
BfoI RGCGCY 1 cut(s) 321
BisI GCNGC 3 cut(s) 89, 92, 364
BlsI GCNGC 3 cut(s) 90, 93, 365
Bme1390I CCNGG 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 130, 178
Bpu10I CCTNAGC 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 370
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 267
Bse1I ACTGG 1 cut(s) 148
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 2 cut(s) 103, 267
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 148
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 2 cut(s) 103, 375
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 103, 267
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 333, 426
BspACI CCGC 2 cut(s) 88, 252
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 319
BspPI GGATC 1 cut(s) 434
BspT107I GGYRCC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 2 cut(s) 333, 426
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 219
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 229
BstACI GRCGYC 1 cut(s) 318
BstC8I GCNNGC 3 cut(s) 43, 58, 361
BstDEI CTNAG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 316
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 2 cut(s) 336, 429
BstMBI GATC 2 cut(s) 333, 426
BstMWI GCNNNNNNNGC 2 cut(s) 42, 290
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 103, 375
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 3 cut(s) 43, 58, 361
CfoI GCGC 1 cut(s) 320
Csp6I GTAC 3 cut(s) 66, 163, 397
CviAII CATG 3 cut(s) 234, 244, 360
CviJI RGCY 8 cut(s) 22, 45, 71, 94, 284, 366, 479, 504
CviKI_1 RGCY 8 cut(s) 22, 45, 71, 94, 284, 366, 479, 504
CviQI GTAC 3 cut(s) 66, 163, 397
DdeI CTNAG 1 cut(s) 192
DinI GGCGCC 1 cut(s) 319
DpnI GATC 2 cut(s) 335, 428
DpnII GATC 2 cut(s) 333, 426
Eco32I GATATC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 369
EcoRV GATATC 1 cut(s) 199
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
FaeI CATG 3 cut(s) 237, 247, 363
FatI CATG 3 cut(s) 233, 243, 359
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 89, 92, 364
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 3 cut(s) 89, 92, 364
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 319
GluI GCNGC 3 cut(s) 89, 92, 364
HaeII RGCGCY 1 cut(s) 321
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 3 cut(s) 237, 247, 363
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 502
HinfI GANTC 1 cut(s) 404
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 1 cut(s) 411
Hpy188III TCNNGA 5 cut(s) 125, 219, 287, 424, 511
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 5 cut(s) 5, 274, 308, 349, 380
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 5 cut(s) 4, 149, 180, 359, 363
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 290
HpyF3I CTNAG 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 3 cut(s) 237, 247, 363
HspAI GCGC 1 cut(s) 318
KasI GGCGCC 1 cut(s) 317
Kzo9I GATC 2 cut(s) 333, 426
LmnI GCTCC 2 cut(s) 65, 99
LpnPI CCDG 8 cut(s) 27, 62, 110, 129, 356, 383, 460, 465
Lsp1109I GCAGC 2 cut(s) 103, 375
LweI GCATC 2 cut(s) 130, 178
MaeI CTAG 1 cut(s) 377
MaeIII GTNAC 1 cut(s) 139
MalI GATC 2 cut(s) 335, 428
MboI GATC 2 cut(s) 333, 426
MboII GAAGA 1 cut(s) 140
MfeI CAATTG 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 2 cut(s) 108, 438
Mly113I GGCGCC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 2 cut(s) 187, 199
MseI TTAA 3 cut(s) 101, 111, 489
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 371
MunI CAATTG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 2 cut(s) 42, 290
NarI GGCGCC 1 cut(s) 318
NdeI CATATG 1 cut(s) 532
NdeII GATC 2 cut(s) 333, 426
NlaIII CATG 3 cut(s) 237, 247, 363
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 450
NspI RCATGY 1 cut(s) 363
PaeI GCATGC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 404
PflMI CCANNNNNTGG 1 cut(s) 103
PkrI GCNGC 3 cut(s) 90, 93, 365
PluTI GGCGCC 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 3 cut(s) 67, 164, 398
RsaNI GTAC 3 cut(s) 66, 163, 397
SaqAI TTAA 3 cut(s) 101, 111, 489
SatI GCNGC 3 cut(s) 89, 92, 364
Sau3AI GATC 2 cut(s) 333, 426
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 151
SetI ASST 7 cut(s) 16, 73, 96, 341, 372, 398, 506
SfaNI GCATC 2 cut(s) 130, 178
SfoI GGCGCC 1 cut(s) 319
SphI GCATGC 1 cut(s) 363
Sse9I AATT 2 cut(s) 108, 438
SsiI CCGC 2 cut(s) 88, 252
SspDI GGCGCC 1 cut(s) 317
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 229
TaqI TCGA 1 cut(s) 299
TasI AATT 2 cut(s) 108, 438
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 3 cut(s) 101, 111, 489
Tru9I TTAA 3 cut(s) 101, 111, 489
TscAI CASTG 1 cut(s) 234
TseI GCWGC 2 cut(s) 91, 363
TspDTI ATGAA 3 cut(s) 327, 331, 496
TspRI CASTG 1 cut(s) 234
Van91I CCANNNNNTGG 1 cut(s) 103
VneI GTGCAC 1 cut(s) 147
XceI RCATGY 1 cut(s) 363
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.