MD17G1127500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006945
Physical Location & Seq
Reverse (-)
11181581 .. 11183782
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1127500.v1.1.491

Sequence Viewer

Length: 2115 bp
ATGAGGCTGAGATCACTATGTGAAAAGGGAAGCCAAGTCTTCAACCAAAATCTGAAGATTACTCGGTTGGGGAAGTCTGGTCGGGTCGCCGAAGCGGTTAAAGTTTTCTCACAAATGACTCAGAGGAACACAGTAACATACAATTCTATGATCTCTGCTTATGCTAAGAATGGTAGAGTTGGTAACGCGCGCCAACTGTTTGATCAAATGCCGCACAGAAACTTGGTTTCTTGGAACACTATGATTTCCGGGTACCTGCACAATGGCGATGTCGAGGAAGCTTATAGGATTTTTATTGACATGCCGGAAAGAGACCTTTACTCGTGGACTTTGATGATAACTTGCTATACACGTAGTGGCGAGCTTGAGAGAGCAAGAGAGCTATTTGATTTGCTTCCGGATAAAGGGGATGCAGCTTGTTGGAATGCAATGATTGCAGGGTATGCGAGGAAGGGGAAGTTTGATGAGGCTAAGGGTTTGTTTGATGAAATGCCAGCCAAAAATTTAGTTTCCTGGAATTCAATGCTTGCAGGGTATACGAAAAATGGGGAGATGCGGCTGGGAGTGAAGTTTTTTGAGGAAATGCCTGAGAGGAATGTGGTTTTGTGGAATTTGATGTTGGATGGGTTTGTTGAGACCCAAATGTGGTTATGTGGGTTTGCGCAAAATGGGGAGATTGCAAAGGCAGAGGATCTCTTTGAGCAGCTGCCAAGTAGAAATGTGGTTTCTTGGAATGCAATGCTTGGAGCCTATGTTCGAGACCACCAAATTGACAAGGCTGTTAAATTATTCAAGGATATGCCTGAGAGGGATTCTGTGTCGTGGACAACAATGATCAATGGGTATGTACGTATTGGTAAGCTTGACGAAGCAAGGCAATTGCTTAACCAAATGCCATACAAGAACATTGCAGCACAAACAGCAATGATGTCTGGATACGTGCAAAATGGAAGGATGGATGAAGCAAGCGAGATCTTCAATCCCATTTCCATCCGTGATGCTGTTTGTTGGAACACAATGATTGCAGGCTATGCTCAGTGTGGGAAAATGGTTGAAGCTCTATCTCTTTTCCGAAAAATGATTAACAAGGACATTGTTTCTTGGAATACTATGATTACTGGTTATAGTCAAACAGGACAAATGGATAAAGCACATGAAATCTTTGAGTCGATGGATGAGAGGAGCGTAGTTTCGTGGAATTCTCTGATTACAGGTTACGTGCAAAATGGGTTATATCTGGATGCACTAAGGAGCATTGTGAAGATGGGACAGGAAGGGAAGAGGCCTGATGAGTCGACTTTTGCATGTGGTCTAAGTGCATGTGCCAATCTTGCAGCTTTACAAGTTGGAAAGAAACTTCACCATTTGGTTGTGAAGTGTGGTTATGTAAATGACTTGTTTGTCAGCAATTCCCTGATTACAATGTATGCTAAAAGCGGGAAAGTTGTGGATGCTAAACTTGTGTTCGAAGATATCAAGTGTGGGGATATTGTTTCTTGGAATTCTTTGATATCTGGGTATGCTCTAAACGGAGATGGTGAAGAGGCAGTAAAGCTCTTCAAAAAGATGCTAATAGAGGGGGTGAATCCAGATCAGGTGACCTTTGTTGCGGTGTTATCAGCATGCAGTCACAGTGAGTTGGTTGAGAGCGGTTTAGAAATTTTTAAGAGCACGACTGAAGTTTACCTTATTGAACCTCTTGCCGAACACTATGCTTGCATGGTTGATCTGTTCGGTCGTGCAGGGAGGCTAGAGGAAGCCTTTGAAATGGTGAGGAATATGAAGATCACGACAACTGCTAGATATGGAGTGCATTACTTGGAGTTTGTAGGATACACCGGAATCTACTACTTGGAAACTTCAAATTATGTGCTCTTGTCGAACATGCATGCTGAGGCTGGCAGATGGGACGAGGTTGAAAGAGTCAGGGTATTAATGAAAGAAAGTAGCACGGAGAAGCAGCCAGGCTGCAGTTGGATTGAACTCAGAAATCAGGTACATGCTTTTCTCTTTGATGATCCGGCGCAGCCCAGAACAGCAGAGCTTGCAAGCATATTAAAGAGTCTAACCACAGAGATGAGAAACACCGGTTACTTGTCTACACCTTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

705

Amino Acids

79.64

Weight (kDa)

5.83

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 42 - 70 3.3e-09 PPR repeat
PPR_2 PF13041 42 - 75 1.4e-08 PPR repeat family
PPR PF01535 45 - 74 1.4e-08 PPR repeat
PPR_2 PF13041 74 - 105 2e-08 PPR repeat family
PPR PF01535 76 - 105 1e-08 PPR repeat
PPR_1 PF12854 137 - 164 8.7e-06 PPR repeat
PPR_2 PF13041 137 - 168 5.4e-07 PPR repeat family
PPR PF01535 140 - 168 8.5e-09 PPR repeat
PPR_2 PF13041 168 - 200 6.4e-07 PPR repeat family
PPR PF01535 170 - 200 4.2e-07 PPR repeat
PPR_2 PF13041 239 - 270 6.7e-08 PPR repeat family
PPR PF01535 242 - 270 1.7e-06 PPR repeat
PPR_1 PF12854 270 - 298 6.8e-07 PPR repeat
PPR_2 PF13041 271 - 303 1.2e-07 PPR repeat family
PPR PF01535 273 - 302 9.5e-08 PPR repeat
PPR_2 PF13041 333 - 364 2.2e-07 PPR repeat family
PPR_1 PF12854 333 - 360 3.8e-06 PPR repeat
PPR PF01535 335 - 364 3.1e-09 PPR repeat
PPR_2 PF13041 363 - 396 1.2e-08 PPR repeat family
PPR PF01535 366 - 395 1.5e-08 PPR repeat
PPR_2 PF13041 395 - 443 2e-07 PPR repeat family
PPR_2 PF13041 496 - 543 7.2e-13 PPR repeat family
PPR_1 PF12854 496 - 523 6.5e-06 PPR repeat
PPR_3 PF13812 496 - 542 2.6e-06 Pentatricopeptide repeat domain
PPR PF01535 498 - 528 1.9e-09 PPR repeat
E_motif PF20431 620 - 661 2.2e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1400
Acc16I TGCGCA 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 264
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 252
AccBSI CCGCTC 1 cut(s) 1650
AccI GTMKAC 3 cut(s) 536, 1295, 2099
AccII CGCG 2 cut(s) 188, 190
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 6 cut(s) 95, 212, 556, 1437, 1610, 1650
AclWI GGATC 2 cut(s) 699, 2012
AcsI RAATTY 6 cut(s) 502, 517, 610, 1198, 1501, 1659
AcuI CTGAAG 2 cut(s) 74, 1698
AdeI CACNNNGTG 2 cut(s) 20, 356
AfaI GTAC 3 cut(s) 254, 849, 1998
AfiI CCNNNNNNNGG 2 cut(s) 404, 645
AflIII ACRYGT 1 cut(s) 350
AgeI ACCGGT 1 cut(s) 2087
AjnI CCWGG 2 cut(s) 512, 1963
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AloI GAACNNNNNNTCC 2 cut(s) 738, 770
Alw21I GWGCWC 2 cut(s) 1673, 1875
Alw26I GTCTC 3 cut(s) 306, 629, 753
AlwI GGATC 2 cut(s) 699, 2012
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 1 cut(s) 1283
ApeKI GCWGC 8 cut(s) 413, 703, 706, 911, 1334, 1960, 1968, 2026
ApoI RAATTY 6 cut(s) 502, 517, 610, 1198, 1501, 1659
AseI ATTAAT 1 cut(s) 1934
AsiGI ACCGGT 1 cut(s) 2087
Asp718I GGTACC 1 cut(s) 252
AspLEI GCGC 4 cut(s) 190, 192, 664, 2026
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 5 cut(s) 1352, 1550, 1594, 1609, 1783
AsuII TTCGAA 1 cut(s) 1467
BanI GGYRCC 1 cut(s) 252
BauI CACGAG 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 2 cut(s) 1673, 1875
BbvCI CCTCAGC 1 cut(s) 1893
BbvI GCAGC 8 cut(s) 425, 693, 715, 923, 1346, 1955, 1972, 2038
BccI CCATC 7 cut(s) 617, 949, 998, 1165, 1258, 1529, 1899
BcgI CGANNNNNNTGC 2 cut(s) 1694, 1728
BciT130I CCWGG 2 cut(s) 514, 1965
BciVI GTATCC 2 cut(s) 929, 1826
BclI TGATCA 2 cut(s) 202, 834
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 3 cut(s) 306, 629, 753
BfaI CTAG 2 cut(s) 1751, 1800
BfmI CTRYAG 1 cut(s) 1969
BfuAI ACCTGC 1 cut(s) 264
BfuI GTATCC 2 cut(s) 929, 1826
BglII AGATCT 1 cut(s) 972
Bme1390I CCNGG 3 cut(s) 250, 514, 1965
BmiI GGNNCC 2 cut(s) 254, 748
BmrFI CCNGG 3 cut(s) 250, 514, 1965
BmsI GCATC 6 cut(s) 400, 543, 988, 1231, 1441, 1557
BpiI GAAGAC 1 cut(s) 31
Bpu10I CCTNAGC 2 cut(s) 471, 1893
Bpu14I TTCGAA 1 cut(s) 1467
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 250
BsaAI YACGTR 4 cut(s) 353, 851, 940, 1219
BsaI GGTCTC 3 cut(s) 306, 629, 753
BsaWI WCCGGW 3 cut(s) 397, 1838, 2087
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 645
Bse118I RCCGGY 1 cut(s) 2087
Bse1I ACTGG 1 cut(s) 1123
Bse3DI GCAATG 4 cut(s) 435, 744, 906, 930
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 2 cut(s) 514, 1965
BseGI GGATG 8 cut(s) 415, 628, 960, 964, 990, 1180, 1246, 1456
BseLI CCNNNNNNNGG 2 cut(s) 404, 645
BseMI GCAATG 4 cut(s) 435, 744, 906, 930
BseMII CTCAG 6 cut(s) 134, 579, 795, 1049, 1884, 1999
BseNI ACTGG 1 cut(s) 1123
BsePI GCGCGC 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 1195
BseXI GCAGC 8 cut(s) 425, 693, 715, 923, 1346, 1955, 1972, 2038
BseYI CCCAGC 1 cut(s) 559
BsgI GTGCAG 2 cut(s) 242, 1761
Bsh1236I CGCG 2 cut(s) 188, 190
Bsh1285I CGRYCG 1 cut(s) 1738
BshFI GGCC 1 cut(s) 1285
BshNI GGYRCC 1 cut(s) 252
BshTI ACCGGT 1 cut(s) 2087
BsiEI CGRYCG 1 cut(s) 1738
BsiHKAI GWGCWC 2 cut(s) 1673, 1875
BsiSI CCGG 6 cut(s) 249, 305, 398, 1839, 2021, 2088
BslFI GGGAC 2 cut(s) 1281, 1922
BslI CCNNNNNNNGG 2 cut(s) 404, 645
BsmAI GTCTC 3 cut(s) 306, 629, 753
BsmFI GGGAC 2 cut(s) 1281, 1922
BsmI GAATGC 2 cut(s) 430, 739
BsnI GGCC 1 cut(s) 1285
Bso31I GGTCTC 3 cut(s) 306, 629, 753
Bsp119I TTCGAA 1 cut(s) 1467
Bsp1286I GDGCHC 2 cut(s) 1673, 1875
Bsp13I TCCGGA 1 cut(s) 397
BspACI CCGC 6 cut(s) 95, 212, 556, 1437, 1610, 1650
BspANI GGCC 1 cut(s) 1285
BspCNI CTCAG 6 cut(s) 133, 580, 796, 1048, 1885, 1998
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 2 cut(s) 188, 190
BspLI GGNNCC 2 cut(s) 254, 748
BspMAI CTGCAG 1 cut(s) 1973
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 2 cut(s) 699, 2012
BspQI GCTCTTC 1 cut(s) 1562
BspT104I TTCGAA 1 cut(s) 1467
BspT107I GGYRCC 1 cut(s) 252
BspTNI GGTCTC 3 cut(s) 306, 629, 753
BsrBI CCGCTC 1 cut(s) 1650
BsrDI GCAATG 4 cut(s) 435, 744, 906, 930
BsrFI RCCGGY 1 cut(s) 2087
BsrI ACTGG 1 cut(s) 1123
BssAI RCCGGY 1 cut(s) 2087
BssHII GCGCGC 1 cut(s) 188
BssNAI GTATAC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 322
Bst1107I GTATAC 1 cut(s) 537
Bst2BI CACGAG 1 cut(s) 322
Bst2UI CCWGG 2 cut(s) 514, 1965
Bst4CI ACNGT 3 cut(s) 133, 198, 1634
Bst6I CTCTTC 3 cut(s) 1274, 1536, 1562
BstAPI GCANNNNNTGC 4 cut(s) 434, 443, 1031, 2045
BstBAI YACGTR 4 cut(s) 353, 851, 940, 1219
BstBI TTCGAA 1 cut(s) 1467
BstEII GGTNACC 1 cut(s) 1597
BstF5I GGATG 8 cut(s) 415, 628, 960, 964, 990, 1180, 1246, 1456
BstFNI CGCG 2 cut(s) 188, 190
BstHHI GCGC 4 cut(s) 190, 192, 664, 2026
BstMAI GTCTC 3 cut(s) 306, 629, 753
BstMCI CGRYCG 1 cut(s) 1738
BstMWI GCNNNNNNNGC 6 cut(s) 434, 443, 920, 1031, 1331, 2045
BstNI CCWGG 2 cut(s) 514, 1965
BstNSI RCATGY 7 cut(s) 304, 1308, 1323, 1626, 1888, 1892, 2003
BstPI GGTNACC 1 cut(s) 1597
BstSCI CCNGG 3 cut(s) 248, 512, 1963
BstSFI CTRYAG 1 cut(s) 1969
BstSNI TACGTA 1 cut(s) 851
BstUI CGCG 2 cut(s) 188, 190
BstV1I GCAGC 8 cut(s) 425, 693, 715, 923, 1346, 1955, 1972, 2038
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 2 cut(s) 691, 972
BstYI RGATCY 2 cut(s) 691, 972
BstZ17I GTATAC 1 cut(s) 537
BsuI GTATCC 2 cut(s) 929, 1826
BsuRI GGCC 1 cut(s) 1285
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 8 cut(s) 415, 628, 960, 964, 990, 1180, 1246, 1456
BtsIMutI CAGTG 2 cut(s) 1043, 1639
BveI ACCTGC 1 cut(s) 264
CfoI GCGC 4 cut(s) 190, 192, 664, 2026
Cfr10I RCCGGY 1 cut(s) 2087
Csp6I GTAC 3 cut(s) 253, 848, 1997
CspAI ACCGGT 1 cut(s) 2087
CviAII CATG 9 cut(s) 301, 1154, 1305, 1320, 1623, 1720, 1885, 1889, 2000
CviQI GTAC 3 cut(s) 253, 848, 1997
DraIII CACNNNGTG 2 cut(s) 20, 356
DrdI GACNNNNNNGTC 1 cut(s) 1400
DseDI GACNNNNNNGTC 1 cut(s) 1400
Eam1104I CTCTTC 3 cut(s) 1274, 1536, 1562
EarI CTCTTC 3 cut(s) 1274, 1536, 1562
Eco105I TACGTA 1 cut(s) 851
Eco147I AGGCCT 1 cut(s) 1285
Eco31I GGTCTC 3 cut(s) 306, 629, 753
Eco32I GATATC 2 cut(s) 1474, 1512
Eco57I CTGAAG 2 cut(s) 74, 1698
Eco91I GGTNACC 1 cut(s) 1597
EcoO65I GGTNACC 1 cut(s) 1597
EcoRI GAATTC 3 cut(s) 517, 1198, 1501
EcoRII CCWGG 2 cut(s) 512, 1963
EcoRV GATATC 2 cut(s) 1474, 1512
EcoT22I ATGCAT 1 cut(s) 1890
FaeI CATG 9 cut(s) 304, 1157, 1308, 1323, 1626, 1723, 1888, 1892, 2003
FalI AAGNNNNNCTT 2 cut(s) 1671, 1703
FaqI GGGAC 2 cut(s) 1281, 1922
FatI CATG 9 cut(s) 300, 1153, 1304, 1319, 1622, 1719, 1884, 1888, 1999
FauI CCCGC 1 cut(s) 1430
FbaI TGATCA 2 cut(s) 202, 834
FblI GTMKAC 3 cut(s) 536, 1295, 2099
FokI GGATG 8 cut(s) 422, 635, 967, 971, 977, 1187, 1253, 1463
FspBI CTAG 2 cut(s) 1751, 1800
FspI TGCGCA 1 cut(s) 663
GlaI GCGC 4 cut(s) 189, 191, 663, 2025
GsaI CCCAGC 1 cut(s) 563
HaeIII GGCC 1 cut(s) 1285
HapII CCGG 6 cut(s) 249, 305, 398, 1839, 2021, 2088
HhaI GCGC 4 cut(s) 190, 192, 664, 2026
Hin1II CATG 9 cut(s) 304, 1157, 1308, 1323, 1626, 1723, 1888, 1892, 2003
Hin6I GCGC 4 cut(s) 188, 190, 662, 2024
HinP1I GCGC 4 cut(s) 188, 190, 662, 2024
HincII GTYRAC 1 cut(s) 1296
HindII GTYRAC 1 cut(s) 1296
HindIII AAGCTT 2 cut(s) 279, 860
HinfI GANTC 8 cut(s) 118, 812, 1166, 1292, 1585, 1842, 1923, 2062
HpaII CCGG 6 cut(s) 249, 305, 398, 1839, 2021, 2088
HphI GGTGA 5 cut(s) 1352, 1550, 1594, 1609, 1783
Hpy166II GTNNAC 6 cut(s) 327, 537, 825, 1296, 1684, 2100
Hpy188I TCNGA 5 cut(s) 54, 123, 1073, 1206, 1988
Hpy188III TCNNGA 6 cut(s) 398, 758, 933, 1238, 1589, 1789
Hpy8I GTNNAC 6 cut(s) 327, 537, 825, 1296, 1684, 2100
HpyAV CCTTC 3 cut(s) 445, 945, 1268
HpyCH4III ACNGT 3 cut(s) 133, 198, 1634
HpyCH4IV ACGT 4 cut(s) 352, 850, 939, 1218
HpyF10VI GCNNNNNNNGC 6 cut(s) 434, 443, 920, 1031, 1331, 2045
HpySE526I ACGT 4 cut(s) 352, 850, 939, 1218
Hsp92II CATG 9 cut(s) 304, 1157, 1308, 1323, 1626, 1723, 1888, 1892, 2003
HspAI GCGC 4 cut(s) 188, 190, 662, 2024
Kpn2I TCCGGA 1 cut(s) 397
KpnI GGTACC 1 cut(s) 256
Ksp22I TGATCA 2 cut(s) 202, 834
LguI GCTCTTC 1 cut(s) 1562
LmnI GCTCC 3 cut(s) 746, 1182, 1251
Lsp1109I GCAGC 8 cut(s) 425, 693, 715, 923, 1346, 1955, 1972, 2038
LweI GCATC 6 cut(s) 400, 543, 988, 1231, 1441, 1557
MaeI CTAG 2 cut(s) 1751, 1800
MaeII ACGT 4 cut(s) 352, 850, 939, 1218
MaeIII GTNAC 6 cut(s) 133, 182, 1214, 1597, 1628, 2090
MbiI CCGCTC 1 cut(s) 1650
MboII GAAGA 9 cut(s) 31, 67, 967, 1273, 1291, 1481, 1549, 1553, 1795
MfeI CAATTG 1 cut(s) 878
MflI RGATCY 2 cut(s) 691, 972
MhlI GDGCHC 2 cut(s) 1673, 1875
MlyI GAGTC 5 cut(s) 112, 1175, 1301, 1932, 2071
MmeI TCCRAC 5 cut(s) 401, 600, 989, 1327, 1955
Mph1103I ATGCAT 1 cut(s) 1890
MroI TCCGGA 1 cut(s) 397
MseI TTAA 7 cut(s) 99, 783, 885, 1083, 1665, 1934, 2057
MslI CAYNNNNRTG 1 cut(s) 2075
MspA1I CMGCKG 1 cut(s) 706
MspI CCGG 6 cut(s) 249, 305, 398, 1839, 2021, 2088
MspR9I CCNGG 3 cut(s) 250, 514, 1965
MunI CAATTG 1 cut(s) 878
Mva1269I GAATGC 2 cut(s) 430, 739
MvaI CCWGG 2 cut(s) 514, 1965
MvnI CGCG 2 cut(s) 188, 190
MwoI GCNNNNNNNGC 6 cut(s) 434, 443, 920, 1031, 1331, 2045
NciI CCSGG 1 cut(s) 250
NlaIII CATG 9 cut(s) 304, 1157, 1308, 1323, 1626, 1723, 1888, 1892, 2003
NlaIV GGNNCC 2 cut(s) 254, 748
NmuCI GTSAC 2 cut(s) 1597, 1628
NsbI TGCGCA 1 cut(s) 663
NsiI ATGCAT 1 cut(s) 1890
NspI RCATGY 7 cut(s) 304, 1308, 1323, 1626, 1888, 1892, 2003
NspV TTCGAA 1 cut(s) 1467
PaeI GCATGC 2 cut(s) 1626, 1892
PauI GCGCGC 1 cut(s) 188
PceI AGGCCT 1 cut(s) 1285
PciSI GCTCTTC 1 cut(s) 1562
PctI GAATGC 2 cut(s) 430, 739
PfeI GAWTC 3 cut(s) 812, 1585, 1842
PfoI TCCNGGA 1 cut(s) 512
PinAI ACCGGT 1 cut(s) 2087
PleI GAGTC 5 cut(s) 112, 1174, 1300, 1931, 2070
PpsI GAGTC 5 cut(s) 112, 1174, 1300, 1931, 2070
Ppu21I YACGTR 4 cut(s) 353, 851, 940, 1219
PshBI ATTAAT 1 cut(s) 1934
Psp6I CCWGG 2 cut(s) 512, 1963
PspEI GGTNACC 1 cut(s) 1597
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 2 cut(s) 512, 1963
PspN4I GGNNCC 2 cut(s) 254, 748
PstI CTGCAG 1 cut(s) 1973
PsuI RGATCY 2 cut(s) 691, 972
PteI GCGCGC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 706
RsaI GTAC 3 cut(s) 254, 849, 1998
RsaNI GTAC 3 cut(s) 253, 848, 1997
RseI CAYNNNNRTG 1 cut(s) 2075
SalI GTCGAC 1 cut(s) 1294
SapI GCTCTTC 1 cut(s) 1562
SaqAI TTAA 7 cut(s) 99, 783, 885, 1083, 1665, 1934, 2057
SchI GAGTC 5 cut(s) 112, 1175, 1301, 1932, 2071
ScrFI CCNGG 3 cut(s) 250, 514, 1965
SduI GDGCHC 2 cut(s) 1673, 1875
SfaNI GCATC 6 cut(s) 400, 543, 988, 1231, 1441, 1557
SfcI CTRYAG 1 cut(s) 1969
SfuI TTCGAA 1 cut(s) 1467
SmiMI CAYNNNNRTG 1 cut(s) 2075
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SnaBI TACGTA 1 cut(s) 851
SphI GCATGC 2 cut(s) 1626, 1892
SseBI AGGCCT 1 cut(s) 1285
SsiI CCGC 6 cut(s) 95, 212, 556, 1437, 1610, 1650
SspMI CTAG 2 cut(s) 1751, 1800
StuI AGGCCT 1 cut(s) 1285
StyD4I CCNGG 3 cut(s) 248, 512, 1963
TaaI ACNGT 3 cut(s) 133, 198, 1634
TaiI ACGT 4 cut(s) 355, 853, 942, 1221
TaqI TCGA 6 cut(s) 273, 757, 1169, 1295, 1467, 1880
TaqII GACCGA 1 cut(s) 1724
TauI GCSGC 2 cut(s) 214, 559
TfiI GAWTC 3 cut(s) 812, 1585, 1842
Tru1I TTAA 7 cut(s) 99, 783, 885, 1083, 1665, 1934, 2057
Tru9I TTAA 7 cut(s) 99, 783, 885, 1083, 1665, 1934, 2057
TscAI CASTG 2 cut(s) 1043, 1639
TseFI GTSAC 2 cut(s) 1597, 1628
TseI GCWGC 8 cut(s) 413, 703, 706, 911, 1334, 1960, 1968, 2026
Tsp45I GTSAC 2 cut(s) 1597, 1628
TspDTI ATGAA 5 cut(s) 501, 975, 1170, 1796, 1952
TspGWI ACGGA 3 cut(s) 983, 1545, 1967
TspRI CASTG 2 cut(s) 1043, 1639
VspI ATTAAT 1 cut(s) 1934
XapI RAATTY 6 cut(s) 502, 517, 610, 1198, 1501, 1659
XceI RCATGY 7 cut(s) 304, 1308, 1323, 1626, 1888, 1892, 2003
XcmI CCANNNNNNNNNTGG 1 cut(s) 1971
XmiI GTMKAC 3 cut(s) 536, 1295, 2099
XspI CTAG 2 cut(s) 1751, 1800
Zsp2I ATGCAT 1 cut(s) 1890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.